Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
proteomic differences between listeria monocytogenes isolates from food and clinical environments.listeria monocytogenes is an organism associated with a wide range of foods. it causes listeriosis, a severe illness that mainly affects people with weakened immune systems. proteomic profiles of three different l. monocytogenes isolates were studied using 1d sds page, 2de and mass spectrometry. the protein banding patterns generated by 1d sds page of three strains of l. monocytogenes were found to be similar. visual observations from 2de gel maps revealed that certain spots appeared to have int ...201425513735
microbiological challenge testing for listeria monocytogenes in ready-to-eat food: a practical approach.food business operators (fbos) are the primary responsible for the safety of food they place on the market. the definition and validation of the product's shelf-life is an essential part for ensuring microbiological safety of food and health of consumers. in the frame of the regulation (ec) no 2073/2005 on microbiological criteria for foodstuffs, fbos shall conduct shelf-life studies in order to assure that their food does not exceed the food safety criteria throughout the defined shelf-life. in ...201427800369
regulation of autophagy by e3 ubiquitin ligase rnf216 through becn1 ubiquitination.autophagy is an evolutionarily conserved biological process involved in an array of physiological and pathological events. without proper control, autophagy contributes to various disorders, including cancer and autoimmune and inflammatory diseases. it is therefore of vital importance that autophagy is under careful balance. thus, additional regulators undoubtedly deepen our understanding of the working network, and provide potential therapeutic targets for disorders. in this study, we found tha ...201425484083
complete genome sequence of listeria monocytogenes lm60, a strain with an enhanced cold adaptation capacity.the complete genome sequence of listeria monocytogenes lm60, a fast cold-adapting serotype 1/2a human isolate, is presented.201425477407
metal ion homeostasis in listeria monocytogenes and importance in host-pathogen interactions.listeria monocytogenes is responsible for one of the most life-threatening food-borne infections and the leading cause of food-poisoning associated deaths in the uk. infection may be of the unborn/newly born infant where disease may manifest as listeric abortion, stillbirth or late-onset neonatal listeriosis, while in adults, infection usually affects the central nervous system causing meningitis. crucial to the survival of l. monocytogenes, both inside and outside the host, is its ability to ac ...201425476765
suspension of thymic emigration promotes the maintenance of antigen-specific memory t cells and the recall responses.thymic involution is evolutionarily conserved and occurs early in life. however, the physiological significance remains elusive of this seemingly detrimental process. the present study investigated the potential impact of altered thymic output on t cell memory using ovalbumin (ova) expressed by listeria monocytogenes as a model antigen. suspension of thymic emigration by thymectomy was shown to lead to a marked increase in the frequency and total number of ova-specific memory t cells. in contras ...201425450389
multivariate analysis reveals differences in biofilm formation capacity among listeria monocytogenes lineages.biofilm formation capacity evaluated under identical conditions differs among listeria monocytogenes lineages. the approach of using one set of factors or one variable at a time fails to explain why some lineages are more prevalent than others in certain environments. this study proposes the use of multivariate analysis to compare biofilm formation by various strains and describes the ecological niches of l. monocytogenes lineages. nutrient availability, temperature, ph and water activity (aw) a ...201425397897
the antimicrobial activity of liquidambar orientalis mill. against food pathogens and antioxidant capacity of leaf extracts.medicinal plants are an important source of substances which are claimed to induce antimicrobial, antimutagenic and antioxidant effects. many plants have been used due to their antimicrobial treatments. antimicrobial and antioxidant activities of l. orientalis have not been reported to the present day. the aim of this work was to investigate of the antimicrobial and antioxidant potentials of different extracts from l. orientalis.201425395700
quantitative proteome analyses identify prfa-responsive proteins and phosphoproteins in listeria monocytogenes.protein phosphorylation is a major mechanism of signal transduction in bacteria. here, we analyzed the proteome and phosphoproteome of a wild-type strain of the food-borne pathogen listeria monocytogenes that was grown in either chemically defined medium or rich medium containing glucose. we then compared these results with those obtained from an isogenic prfa* mutant that produced a constitutively active form of prfa, the main transcriptional activator of virulence genes. in the prfa* mutant gr ...201425383790
intestinal expression of fas and fas ligand is upregulated by bacterial signaling through tlr4 and tlr5, with activation of fas modulating intestinal tlr-mediated inflammation.tlrs play an important role in mediating intestinal inflammation and homeostasis. fas is best studied in terms of its function in apoptosis, but recent studies demonstrate that fas signaling may mediate additional functions such as inflammation. the role of fas, and the fas ligand (fasl), in the intestine is poorly understood. the aim of this study was to evaluate potential cross-talk between tlrs and fas/fasl system in intestinal epithelial cells (iecs). iecs were stimulated with tlr ligands, a ...201425378591
in situ proteolysis, crystallization and preliminary x-ray diffraction analysis of a vhh that binds listeria internalin b.the variable region of camelid heavy-chain antibodies produces the smallest known antibody fragment with antigen-binding capability (a vhh). the vhh r303 binds internalin b (inlb), a virulence factor expressed by the pathogen listeria monocytogenes. inlb is critical for initiation of listeria infection, as it binds a receptor (c-met) on epithelial cells, triggering the entry of bacteria into host cells. inlb is surface-exposed and is required for virulence, hence a vhh targeting inlb has potenti ...201425372824
prevalence and populations of listeria monocytogenes in meat products retailed in sao paulo, brazil.this study evaluated the prevalence of the populations and serotypes of listeria monocytogenes in 552 refrigerated samples of ground beef, chicken leg, hot dog, and pork sausage collected in supermarkets in the city of sao paulo, sp, brazil, between may 2008 and july 2009. the supermarkets were selected after stratification by geographical region and by random draw. tests for presence and enumeration of l. monocytogenes were based on iso 11290-1:1996/amd.1:2004 and iso 11290-2:1998 methods, resp ...201425407460
analysis of carbon substrates used by listeria monocytogenes during growth in j774a.1 macrophages suggests a bipartite intracellular metabolism.intracellular bacterial pathogens (ibps) are dependent on various nutrients provided by the host cells. different strategies may therefore be necessary to adapt the intracellular metabolism of ibps to the host cells. the specific carbon sources, the catabolic pathways participating in their degradation, and the biosynthetic performances of ibps are still poorly understood. in this report, we have exploited the technique of (13)c-isotopologue profiling to further study the carbon metabolism of li ...201425405102
two outbreaks of listeria monocytogenes infection, northern spain.in the province of gipuzkoa, spain (≈700,000 inhabitants), 7-12 episodes of human listeriosis were recorded annually during 2009-2012. however, during january 2013-february 2014, 27 episodes were detected, including 11 pregnancy-associated cases. fifteen cases in 2 epidemiologically unrelated outbreaks were caused by a rare type of listeria monocytogenes, sequence type 87 serotype 1/2b.201425418800
splenectomy alters distribution and turnover but not numbers or protective capacity of de novo generated memory cd8 t-cells.the spleen is a highly compartmentalized lymphoid organ that allows for efficient antigen presentation and activation of immune responses. additionally, the spleen itself functions to remove senescent red blood cells, filter bacteria, and sequester platelets. splenectomy, commonly performed after blunt force trauma or splenomegaly, has been shown to increase risk of certain bacterial and parasitic infections years after removal of the spleen. although previous studies report defects in memory b- ...201425414706
committee opinion no. 614: management of pregnant women with presumptive exposure to listeria monocytogenes.listeriosis is predominantly a foodborne illness, with sporadic and outbreak-related cases tied to consumption of food contaminated with listeria (listeria monocytogenes). the incidence of listeriosis associated with pregnancy is approximately 13 times higher than in the general population. maternal infection may present as a nonspecific, flu-like illness with fever, myalgia, backache, and headache, often preceded by diarrhea or other gastrointestinal symptoms. however, fetal and neonatal infect ...201425411758
[sausage-related listeria monocytogenes infection].infection with listeria monocytogenes is rare and mainly seen in immunosuppressed patients. infection with l. monocytogenes has a mortality rate of 30%. we present a case report of l. monocytogenes bacteraemia and endocarditis in a 70-year-old man with several co-morbidities and following four major surgical procedures. this illustrates the findings and characteristics in one of the 16 patients who died in 2013 and 2014 this summer due to sausage-related l. monocytogenes infection.201425430580
listeria monocytogenes-associated biliary tract infections: a study of 12 consecutive cases and review.at present, little is known regarding listeria monocytogenes-associated biliary tract infection, a rare form of listeriosis.in this article, we will study 12 culture-proven cases reported to the french national reference center for listeria from 1996 to 2013 and review the 8 previously published cases.twenty cases were studied: 17 cholecystitis, 2 cholangitis, and 1 biliary cyst infection. half were men with a median age of 69 years (32-85). comorbidities were present in 80%, including cirrhosis ...201425319439
novel mechanism of hemin capture by hbp2, the hemoglobin-binding hemophore from listeria monocytogenes.iron is an essential nutrient that is required for the growth of the bacterial pathogen listeria monocytogenes. in cell cultures, this microbe secretes hemin/hemoglobin-binding protein 2 (hbp2; lmo2185) protein, which has been proposed to function as a hemophore that scavenges heme from the environment. based on its primary sequence, hbp2 contains three near transporter (neat) domains of unknown function. here we show that each of these domains mediates high affinity binding to ferric heme (hemi ...201425315777
direct incorporation of the nkt-cell activator α-galactosylceramide into a recombinant listeria monocytogenes improves breast cancer vaccine efficacy.immune suppression in the tumour microenvironment remains a major limitation to successful immunotherapy of cancer. in the current study, we analysed whether the natural killer t cell-activating glycolipid α-galactosylceramide could overcome immune suppression and improve vaccination against metastatic breast cancer.201425314062
the listeria monocytogenes transposon tn6188 provides increased tolerance to various quaternary ammonium compounds and ethidium bromide.tolerance of the foodborne pathogen listeria monocytogenes to sublethal concentrations of disinfectants has been frequently reported. particularly, quaternary ammonium compounds (qacs) such as benzalkonium chloride (bc) are often used in disinfectants and also as antiseptics in food industry and hospitals. recently, we described tn6188, a novel transposon in l. monocytogenes harbouring the transporter qach, a molecular mechanism leading to increased tolerance to bc. in this study, we investigate ...201425312720
pregnancy-related listeriosis in france, 1984 to 2011, with a focus on 606 cases from 1999 to 2011.this study describes trends in the incidence of pregnancy-related listeriosis in france between 1984 and 2011, and presents the major characteristics of 606 cases reported between 1999 and 2011 to the french institute for public health surveillance through the mandatory notification system. the incidence of pregnancy-related listeriosis decreased by a factor of 12 from 1984 to 2011. this reduction was a result of progressive implementation of specific listeria monocytogenes control measures in f ...201425306879
impedimetric characterization of adsorption of listeria monocytogenes on the surface of an aluminum-based immunosensor.the impedimetric characteristics of an immunosensor depend on the electrical properties of an immunosensor substrate. the impedimetric characteristics of an immunosensor compared with adsorption of listeria monocytogenes were investigated on an aluminum surface insulated with an electrically resistive aluminum oxide layer. antibody for l. monocytogenes (anti-l. monocytogenes) was immobilized on an aluminum surface that was insulated with a native air-formed aluminum oxide layer. the resistance o ...201425296910
detection of very long antisense transcripts by whole transcriptome rna-seq analysis of listeria monocytogenes by semiconductor sequencing technology.the gram-positive bacterium listeria monocytogenes is the causative agent of listeriosis, a severe food-borne infection characterised by abortion, septicaemia, or meningoencephalitis. l. monocytogenes causes outbreaks of febrile gastroenteritis and accounts for community-acquired bacterial meningitis in humans. listeriosis has one of the highest mortality rates (up to 30%) of all food-borne infections. this human pathogenic bacterium is an important model organism for biomedical research to inve ...201425286309
inhibition of listeria monocytogenes in deli-style turkey breast formulated with cultured celery powder and/or cultured sugar-vinegar blend during storage at 4°c.fermentation-derived nitrite (no2) from vegetable sources is increasingly used as a "clean label" alternative to conventional nano2. previous results suggested that processed meats cured with no2 derived from a "natural" source had lower antimicrobial activity than did meats produced with chemical nano2; however, the differences were likely due to no2 concentration rather than source. the objective of this study was to compare the antilisterial properties of traditional and clean label alternati ...201425285499
decontamination of hard cheeses by pulsed uv light.cheese is a ready-to-eat food that may be contaminated on the surface by undesirable spoilage and pathogenic microorganisms during production, packaging, and postpackaging processes. penicillium roqueforti is commonly found on cheese surfaces at refrigeration temperatures and is one of the most common spoilage fungal species. consumption of cheese contaminated with listeria monocytogenes can result in foodborne listeriosis. therefore, cheese should be decontaminated at postprocessing stages. pul ...201425285489
modeling the effects of temperature, sodium chloride, and green tea and their interactions on the thermal inactivation of listeria monocytogenes in turkey.the interactive effects of heating temperature (55 to 65°c), sodium chloride (nacl; 0 to 2%), and green tea 60% polyphenol extract (gtpe; 0 to 3%) on the heat resistance of a five-strain mixture of listeria monocytogenes in ground turkey were determined. thermal death times were quantified in bags that were submerged in a circulating water bath set at 55, 57, 60, 63, and 65°c. the recovery medium was tryptic soy agar supplemented with 0.6% yeast extract and 1% sodium pyruvate. d-values were anal ...201425285486
bacterial and cellular rnas at work during listeria infection.listeria monocytogenes is an intracellular pathogen that can enter and invade host cells. in the course of its infection, rna-mediated regulatory mechanisms provide a fast and versatile response for both the bacterium and the host. they regulate a variety of processes, such as environment sensing and virulence in pathogenic bacteria, as well as development, cellular differentiation, metabolism and immune responses in eukaryotic cells. the aim of this article is to summarize first the rna-mediate ...201425340833
foodborne illness, australia, circa 2000 and circa 2010.foodborne disease is a major public health problem worldwide. to examine changes in foodborne illness in australia, we estimated the incidence, hospitalizations, and deaths attributed to contaminated food circa 2010 and recalculated estimates from circa 2000. approximately 25% of gastroenteritis cases were caused by contaminated food; to account for uncertainty we used simulation techniques to estimate 90% credible intervals. we estimate that circa 2010, 4.1 million foodborne gastroenteritis cas ...201425340705
of microspheres and microbes: a double-hit strategy for cancer immunotherapy.efficient immunotherapy relies on the rapid generation of elevated amounts of cancer-specific t lymphocytes. we have recently demonstrated that both rapid and potent tumor-targeting immune responses can be induced with a heterologous prime-boost regimen consisting of poly-lactic co-glycolic acid (plga) microsphere-based immunization followed by listeria monocytogenes infection.201425340000
geographical and meteorological factors associated with isolation of listeria species in new york state produce production and natural environments.listeria species have been isolated from diverse environments, often at considerable prevalence, and are known to persist in food processing facilities. the presence of listeria spp. has been suggested to be a marker for listeria monocytogenes contamination. therefore, a study was conducted to (i) determine the prevalence and diversity of listeria spp. in produce production and natural environments and (ii) identify geographical and/or meteorological factors that affect the isolation of listeria ...201425364926
inactivation of pathogens during aerobic composting of fresh and aged dairy manure and different carbon amendments.two separate studies were conducted to address the condition and the type of feedstocks used during composting of dairy manure. in each study, physical (temperature), chemical (ammonia, volatile acids, and ph), and biological (salmonella, listeria monocytogenes, and escherichia coli o157:h7) parameters were monitored during composting in bioreactors to assess the degree to which they were affected by the experimental variables and, ultimately, the ability of the chemical and physical parameters ...201425364925
genetic and phenotypic characterization of listeria monocytogenes from human clinical cases that occurred in portugal between 2008 and 2012.listeria monocytogenes infection (listeriosis) is an uncommon but severe foodborne illness that affects mainly individuals with recognized underlying conditions: the elderly, immunocompromised individuals, and pregnant women and their fetuses. the aim of this study was to obtain epidemiological data on cases of listeriosis occurring in portugal from 2008 through 2012, collected in hospitals on a voluntary basis. l. monocytogenes isolates were characterized by genoserotyping by multiplex polymera ...201425361468
the listeria small rna rli27 regulates a cell wall protein inside eukaryotic cells by targeting a long 5'-utr variant.listeria monocytogenes is a bacterial pathogen whose genome encodes many cell wall proteins that bind covalently to peptidoglycan. some members of this protein family have a key role in virulence, and recent studies show that some of these, such as lmo0514, are upregulated in bacteria that colonize eukaryotic cells. the regulatory mechanisms that lead to these changes in cell wall proteins remain poorly characterized. here we studied the regulation responsible for increased lmo0514 protein level ...201425356775
listeria infection in chinese pregnant women and neonates from shandong.this study aims to investigate the characters of pregnant women and neonates with listeria monocytogenes infections (l. monocytogenes) in shandong of china.201425356132
detection of listeria monocytogenes in food samples in avezzano, sulmona and castel di sangro (province of l'aquila, abruzzo, italy) between 2000-2009.the retrospective study of the results of the analysed samples is a fundamental tool for the identification of major risk related to food and for planning future monitoring activities. the evaluation of the quality of data collected may also allow for estimating the effectiveness of the controls so to improve their efficacy. in this article, the authors evaluated the results of tests for the detection of listeria monocytogenes performed by the istituto zooprofilattico sperimentale dell'abruzzo e ...201425273959
synergetic effect of combined fumaric acid and slightly acidic electrolysed water on the inactivation of food-borne pathogens and extending the shelf life of fresh beef.to evaluate synergetic effect of slight acidic electrolysed water (saew) and fumaric acid (fa) on inactivation of total viable count (tvc) and staphylococcus aureus, listeria monocytogenes, escherichia coli o157:h7 and salmonella typhimurium in fresh beef and to study shelf life and sensory quality of beef.201425273314
rapid and sensitive detection of listeria monocytogenes by cross-priming amplification of lmo0733 gene.listeria monocytogenes is a food-borne pathogen that causes severe opportunistic infection in humans and animals. this study reports the development of single cross-priming amplification (s-cpa) and double cpa (d-cpa) assays targeting species-specific gene lmo0733 for identifying l. monocytogenes strains. the cpa assays were performed at a constant temperature 64 °c using seven specific primers and evaluated for specificity and sensitivity. the color change of positive amplification was directly ...201425273275
tsc1 promotes the differentiation of memory cd8+ t cells via orchestrating the transcriptional and metabolic programs.memory cd8(+) t cells are an essential component of protective immunity. signaling via mechanistic target of rapamycin (mtor) has been implicated in the regulation of the differentiation of effector and memory t cells. however, little is understood about the mechanisms that control mtor activity, or the effector pathways regulated by mtor. we describe here that tuberous sclerosis 1 (tsc1), a regulator of mtor signaling, plays a crucial role in promoting the differentiation and function of memory ...201425271321
application of high resolution pyrolysis gas chromatography/mass spectrometry (hrpgc/ms) for detecting listeria monocytogenes.the characteristic of listeria monocytogenes' pyrolysis product was found by fingerprint analysis of high resolution pyrolysis gas chromatography and mass spectrometry (hrpgc/ms), which hold a great potential to rapidly detect l. monocytogenes with the application of selected ion monitoring (sim). food products (beef and milk) contaminated by l. monocytogenes and uncontaminated were evaluated. the retention time of the characteristic peak of pyrolysis product was 19.056min, the ion of m/z were 5 ...201425270059
σ(b) affects biofilm formation under the dual stress conditions imposed by adding salt and low temperature in listeria monocytogenes.the food-borne pathogenic bacteria listeria monocytogenes can form biofilms on various surfaces including food-processing equipment. biofilms offer survival benefits to the organisms entrapped against environmental insults. moreover, the σ(b) transcription factor of l. monocytogenes plays an important role in its survival under various stress conditions. in this study, we evaluated whether σ(b) contributes to biofilm formation when l. monocytogenes is grown under various temperatures and media. ...201425269605
chaperone-mediated autophagy regulates t cell responses through targeted degradation of negative regulators of t cell activation.chaperone-mediated autophagy (cma) targets soluble proteins for lysosomal degradation. here we found that cma was activated in t cells in response to engagement of the t cell antigen receptor (tcr), which induced expression of the cma-related lysosomal receptor lamp-2a. in activated t cells, cma targeted the ubiquitin ligase itch and the calcineurin inhibitor rcan1 for degradation to maintain activation-induced responses. consequently, deletion of the gene encoding lamp-2a in t cells caused defi ...201425263126
bacteria detection based on the evolution of enzyme-generated volatile organic compounds: determination of listeria monocytogenes in milk samples.the rapid detection of listeria monocytogenes contamination in food is essential to prevent food-borne illness in humans. the aim of this study was to differentiate non-contaminated milk from milk contaminated with l. monocytogenes using enzyme substrates coupled with the analysis of volatile organic compounds (vocs). the method is based on the activity of β-glucosidase and hippuricase enzymes and the detection of a specific voc i.e. 2-nitrophenol and 3-fluoroaniline, respectively. vocs were ext ...201425263120
adhesion of salmonella enteritidis and listeria monocytogenes on stainless steel welds.pathogenic microorganisms are able to adhere on equipment surfaces, being possible to contaminate food during processing. salmonella spp. and listeria monocytogenes are important pathogens that can be transmitted by food, causing severe foodborne diseases. most surfaces of food processing industry are made of stainless steel joined by welds. however currently, there are few studies evaluating the influence of welds in the microorganism's adhesion. therefore the purpose of the present study was t ...201425261827
whole-genome sequence of listeria monocytogenes type strain 53 xxiii.listeria monocytogenes causes the food-borne illness listeriosis that primarily infects "vulnerable" groups (e.g., elderly adults, pregnant women, very young children, and the immunocompromised). we sequenced the genome of l. monocytogenes xxiii (atcc 15313) and assembled it into a single scaffold (three contigs) and deposited the annotated assembly into genbank as accession no. joox00000000.201425258271
[immunomodulatory role of dietary lipids in an immunosuppressed mouse model and infected with listeria monocytogenes].dietary fatty acids immunomodulatory capacity in immunosuppression conditions may differ according to the type of fatty acid present in the diet.201425335671
salmonella enterica serovar enteritidis and listeria monocytogenes in mango (mangifera indica l.) pulp: growth, survival and cross-contamination.this study examined the ability of salmonella enterica serovar enteritidis and listeria monocytogenes to grow or survive in mango pulp stored at -20°c, 4°c, 10°c and 25°c, as well as to cross-contaminate mangoes by means of a knife contaminated with different levels of these pathogens.201425328926
current status of antisense rna-mediated gene regulation in listeria monocytogenes.listeria monocytogenes is a gram-positive human-pathogen bacterium that served as an experimental model for investigating fundamental processes of adaptive immunity and virulence. recent novel technologies allowed the identification of several hundred non-coding rnas (ncrnas) in the listeria genome and provided insight into an unexpected complex transcriptional machinery. in this review, we discuss ncrnas that are encoded on the opposite strand of the target gene and are therefore termed antisen ...201425325017
drastic therapy for listerial brain abscess involving combined hyperbaric oxygen therapy and antimicrobial agents.listeria monocytogenes (l. monocytogenes) is a rare causative pathogen of brain abscess that is often found in immunocompromised patients. although patients with supratentorial listerial abscesses showed a longer survival with surgical drainage, the standard therapy for patients with subtentorial lesions has not been established.201425324887
phosphotransferase system-dependent extracellular growth of listeria monocytogenes is regulated by alternative sigma factors σl and σh.alternative sigma (σ) factors and phosphotransferase systems (ptss) play pivotal roles in the environmental adaptation and virulence of listeria monocytogenes. the growth of the l. monocytogenes parent strain 10403s and 15 isogenic alternative σ factor mutants was assessed in defined minimal medium (dm) with pts-dependent or non-pts-dependent carbon sources at 25°c or 37°c. overall, our results suggested that the regulatory effect of alternative σ factors on the growth of l. monocytogenes is dep ...201425281379
[effect of ultra high pressure on cell membrane destruction and death of listeria monocytogenes].to study the effect of ultra high pressure (uhp) on the cell membrane of listeria monocytogenes.201425252455
the adaptor asc exacerbates lethal listeria monocytogenes infection by mediating il-18 production in an inflammasome-dependent and -independent manner.listeria monocytogenes induces the formation of inflammasomes and subsequent caspase-1 activation, and the adaptor apoptosis-associated speck-like protein containing a card (asc) is crucial for this response. however, the role of asc in l. monocytogenes infection in vivo is unclear. in this study, we demonstrate that asc has a detrimental effect on host defense against l. monocytogenes infection at a lethal dose (10(6) cfu), but not at a sublethal dose (10(3) cfu). during lethal l. monocytogenes ...201425251560
modeling the growth of listeria monocytogenes on the surface of smear- or mold-ripened cheese.surface-ripened cheeses are matured by means of manual or mechanical technologies posing a risk of cross-contamination, if any cheeses are contaminated with listeria monocytogenes. in predictive microbiology, primary models are used to describe microbial responses, such as growth rate over time and secondary models explain how those responses change with environmental factors. in this way, primary models were used to assess the growth rate of l. monocytogenes during ripening of the cheeses and t ...201425072033
systemic listeria monocytogenes infection in a 2-year-old immunocompetent child.central nervous system infection and sepsis are the most frequently observed clinical presentations of listeriosis infection; however, they are rare in immunocompetent children beyond the neonatal period. in the presented case, we described gastrointestinal involvement, subacute meningitis, sinusitis and sepsis in a two-year-old previously healthy child with acute infection caused by listeria monocytogenes. we suggest that the infection was probably enhanced by an inappropriate corticosteroid tr ...201425060681
draft genome sequence of listeria monocytogenes strain li0521 (syn. hpb7171), isolated in 1983 during an outbreak in massachusetts caused by contaminated cheese.listeria monocytogenes, a pathogenic food-borne bacterium, is the causative agent of both sporadic and outbreak cases of human listeriosis. here, we present the genome sequence of l. monocytogenes reference strain li0521, isolated during an outbreak involving contaminated cheese, which has been used as the model during several proteomic studies.201425059873
listeria meningitis and resultant symptomatic hydrocephalus complicating infliximab treatment for ulcerative colitis.listeria monocytogenes, an uncommon pathogen, should be considered by clinicians as a source of sepsis and meningitis in the immunocompromised individuals, including those on anti-tnf alpha agents. immunosuppressant agents including biologic therapies have transformed the management of various rheumatological and dermatological conditions.(1) we report a case of life-threatening l. monocytogenes sepsis and meningitis in a 75-year-old man receiving infliximab for severe ulcerative colitis (uc).201425057381
genetic classification of listeria monocytogenes serotype 4b strains, including epidemic clones, isolated from retail meat in the tokyo metropolitan area.a food-borne pathogen, listeria monocytogenes serotype 4b, has been frequently isolated from patients with listeriosis, and numerous outbreaks of listeriosis are associated with this serotype. in the present study, we performed subtyping of l. monocytogenes serotype 4b strains on the basis of genetic analyses. thirty-four isolates of serotype 4b were classified into 8 genotypes, namely genotypes 12, 15, 16, 17, 18, 23, 24, and 25, on the basis of the sequence for the partial iap gene. genetic an ...201425056070
instantlabs listeria monocytogenes food safety kit. performance tested method 051304.the instantlabs listeria monocytogenes food safety kit was validated against the international organization for standardization (iso) reference method 11290-1 for the detection of listeria monocytogenes and other listeria species. the matrixes (stainless steel, sealed concrete, ice cream, whole milk, cheddar cheese, raw shrimp, hot dogs, deli turkey, and lettuce) were inoculated with approximately 1 cfu/test portion of l. monocytogenes to generate fractional positives (5-15) in 20 inoculated sam ...201425051634
instantlabs listeria species food safety kit. performance tested methods 041304.the instantlabs listeria species food safetykitwas validated against the international organization for standardization (iso) reference method 11290-1 for the detection of listeria monocytogenes and other listeria species. the matrixes (stainless steel, sealed concrete, cheddar cheese, raw shrimp, and hot dogs) were inoculated with approximately 1 cfu/test portion of various listeria species to generate fractional positives (5-15) in 20 inoculated samples. enrichments were also fractionally inoc ...201425051633
deletion of the membrane protein lmo0412 increases the virulence of listeria monocytogenes.listeria monocytogenes is a facultative intracellular pathogen that causes gastroenteritis, meningitis, encephalitis and maternofetal infections. 20-30% of eubacterial orfs are predicted to encode membrane proteins. the bacterial cytoplasmic membrane is a macromolecular structure, which plays a key role for the pathogenesis. despite this, little knowledge exists regarding the function of cytoplasmic membrane proteins of listeria during infection. here, we investigated a predicted membrane protei ...201425051038
assessment of oligogalacturonide from citrus pectin as a potential antibacterial agent against foodborne pathogens.foodborne diseases are an important public health problem in the world. the bacterial resistance against presently used antibiotics is becoming a public health issue; hence, the discovery of new antimicrobial agents from natural sources attracts a lot of attention. antibacterial activities of oligogalacturonide from commercial microbial pectic enzyme (cpe) treated citrus pectin, which exhibits antioxidant and antitumor activities, against 4 foodborne pathogens including salmonella typhimurium, s ...201425048440
serogrouping and pulsed-field gel electrophoresis analysis of listeria monocytogenes isolates from cases of human infection in hungary 2004–2012 molecular typing of listeria monocytogenes in hungary.eighty isolates of listeria monocytogenes cultured from human infections in hungary between 2004 and 2012 were serotyped by the pcr technique of doumithet al. [9] and characterised by pulsed-field gel electrophoresis (pfge). most of the isolates belonged to two serogroups: 53 isolates (66.3%) to serovar group 4b,4d,4e and 21isolates (25.8%) to serogroup 1/2a,3a. although many pulsotypes were identified a particular pulsotype proved highly excelling comprising of 31 isolates after digestion by bo ...201425046881
dnase i and proteinase k impair listeria monocytogenes biofilm formation and induce dispersal of pre-existing biofilms.current sanitation methods in the food industry are not always sufficient for prevention or dispersal of listeria monocytogenes biofilms. here, we determined if prevention of adherence or dispersal of existing biofilms could occur if biofilm matrix components were disrupted enzymatically. addition of dnase during biofilm formation reduced attachment (<50% of control) to polystyrene. treatment of established 72h biofilms with 100μg/ml of dnase for 24h induced incomplete biofilm dispersal, with <2 ...201425043896
use of mild irradiation doses to control pathogenic bacteria on meat trimmings for production of patties aiming at provoking minimal changes in quality attributes.the objectives of the present work were to assess the use of moderate doses of gamma irradiation (2 to 5 kgy) and to reduce the risk of pathogen presence without altering the quality attributes of bovine trimmings and of patties made of irradiated trimmings. microbiological indicators (coliforms, pseudomonas spp and mesophilic aerobic counts), physicochemical indicators (ph, color and tiobarbituric acid) and sensory changes were evaluated during storage. 5 kgy irradiation doses slightly increase ...201425042241
inhibitory effects of the κ-casein macropeptide isolated from milk protein on the biofilm formation and virulence of listeria monocytogenes.we demonstrate the inhibitory effects of κ-casein macropeptide (cmp) on the biofilm formation and virulence of listeria monocytogenes scott a. the inhibition of biofilm formation by cmp was initially investigated by using the protocol applied for the 96-well microtiter plate assay. low concentrations of cmp (0.1, 0.2, 0.3, 0.4, and 0.5 mg/ml) that were tested resulted in a profound inhibitory effect on biofilm formation at a concentration of 0.4 mg/ml. cmp also significantly repressed the transc ...201425036840
serial transfer of single-cell-derived immunocompetence reveals stemness of cd8(+) central memory t cells.maintenance of immunological memory has been proposed to rely on stem-cell-like lymphocytes. however, data supporting this hypothesis are focused on the developmental potential of lymphocyte populations and are thus insufficient to establish the functional hallmarks of stemness. here, we investigated self-renewal capacity and multipotency of individual memory lymphocytes by in vivo fate mapping of cd8(+) t cells and their descendants across three generations of serial single-cell adoptive transf ...201425035956
the effect of oleanolic and ursolic acids on the hemolytic properties and biofilm formation of listeria monocytogenes.oleanolic acid and ursolic acid are pentacyclic triterpenoids isolated from a variety of medicinal plants, which have antibacterial activity. listeria monocytogenes is a gram-positive facultative pathogen, being the causative agent of listeriosis. the present study was carried out to evaluate the in vitro effect of sub-inhibitory concentrations of both triterpene acids on the pathogenicity determinants of l. monocytogenes: their hemolytic activity and biofilm forming ability. oleanolic and ursol ...201425033658
the first invertebrate rig-i-like receptor (rlr) homolog gene in the pacific oyster crassostrea gigas.retinoic acid-inducible gene i (rig-i)-like receptor (rlr) is a pivotal receptor that detects numerous rna and dna viruses and mediates the innate induction of interferons and pro-inflammatory cytokines upon viral infection. in the present study, we cloned and characterized the first rig-i gene in a marine mollusk, crassostrea gigas, and designated it as cgrig-i. the full-length cgrig-i cdna is 3436 bp, including 5'- and 3'-untranslated regions (utrs) of 93 bp and 286 bp, respectively, and an op ...201425107697
effect of lauric arginate, nisin z, and a combination against several food-related bacteria.the effects of lauric arginate (lae) and nisin z, alone or in combination, on cell damage were investigated against escherichia coli o157:h7, listeria monocytogenes and brochothrix thermosphacta, by scanning electron microscopy (sem) and transmission electron microscopy (tem) observations, efflux of potassium and phosphate ions, and growth inhibition. a combination of lae with nisin z caused severe and dramatic changes in the cytoplasmic membrane and cell lysis of both gram-positive and gram-neg ...201425106040
cyclic di-gmp-dependent signaling pathways in the pathogenic firmicute listeria monocytogenes.we characterized key components and major targets of the c-di-gmp signaling pathways in the foodborne pathogen listeria monocytogenes, identified a new c-di-gmp-inducible exopolysaccharide responsible for motility inhibition, cell aggregation, and enhanced tolerance to disinfectants and desiccation, and provided first insights into the role of c-di-gmp signaling in listerial virulence. genome-wide genetic and biochemical analyses of c-di-gmp signaling pathways revealed that l. monocytogenes has ...201425101646
management of pregnant women with presumptive exposure to listeria monocytogenes.: listeriosis is predominantly a foodborne illness, with sporadic and outbreak-related cases tied to consumption of food contaminated with listeria (listeria monocytogenes). the incidence of listeriosis associated with pregnancy is approximately 13 times higher than in the general population. maternal infection may present as a nonspecific, flu-like illness with fever, myalgia, backache, and headache, often preceded by diarrhea or other gastrointestinal symptoms. however, fetal and neonatal infe ...201425098360
pediococcus pentosaceus sb83 as a potential probiotic incorporated in a liquid system for vaginal delivery.pediococcus pentosaceus sb83 is a bacteriocinogenic culture having potential use as a vaginal probiotic. the objective of this study was to evaluate the behaviour of p. pentosaceus sb83 incorporated in a liquid system for eventually formulating a gel for vaginal delivery. the vaginal probiotic incorporated into glycerol was able to survive in simulated vaginal fluid at normal vaginal ph (4.2) and at ph of vaginal infections (5.5 and 6.5). the probiotic can be stored at 4 °c for a longer period o ...201425097107
small molecule deubiquitinase inhibitors promote macrophage anti-infective capacity.the global spread of anti-microbial resistance requires urgent attention, and diverse alternative strategies have been suggested to address this public health concern. host-directed immunomodulatory therapies represent one approach that could reduce selection for resistant bacterial strains. recently, the small molecule deubiquitinase inhibitor wp1130 was reported as a potential anti-infective drug against important human food-borne pathogens, notably listeria monocytogenes and noroviruses. util ...201425093325
integrated air stream micromixer for performing bioanalytical assays on a plastic chip.this paper describes the design, functioning and use of an integrated mixer that relies on air flux to agitate microliter entities of fluid in an embedded microfluidic cavity. the system was fabricated from multiple layers of a thermoplastic elastomer and features circuits for both liquid and air supply along with pneumatic valves for process control. internally-dyed polymer particles have been used to visualize flow within the fluid phase during agitation. numerical modelling of the micromixer ...201425091476
impact of rli87 gene deletion on response of listeria monocytogenes to environmental stress.listeria monocytogenes (lm) is a zoonotic pathogen that widely adapts to various environments. recent studies have found that noncoding rnas (ncrnas) play regulatory roles in lm responses to environmental stress. to understand the role of ncrna rli87 in the response regulation, a rli87 deletion strain lm-δrli87 was constructed by homologous recombination and tested for stress responses to high temperature, low temperature, high osmotic pressure, alcohol, acidity, alkaline and oxidative environme ...201425091276
genomic characterization and expression analysis of five novel il-17 genes in the pacific oyster, crassostrea gigas.interleukin-17 (il-17) is a proinflammatory cytokine that plays an important role in clearing extracellular bacteria and contributes to the pathology of many autoimmune and allergic conditions. in the present study, five novel il-17 homologs were identified by searching and analyzing the pacific oyster genome. all six cgil-17 members (including a previously reported homolog) contained four conserved cysteines that were used in the formation of disulfide bonds. phylogenetic analysis showed that a ...201425090939
use of antimicrobial films and edible coatings incorporating chemical and biological preservatives to control growth of listeria monocytogenes on cold smoked salmon.the relatively high incidence of listeria monocytogenes in cold smoked salmon (css) is of concern as it is a refrigerated processed food of extended durability (repfed). the objectives of this study were to compare and optimize the antimicrobial effectiveness of films and coatings incorporating nisin (nis) and sodium lactate (sl), sodium diacetate (sd), potassium sorbate (ps), and/or sodium benzoate (sb) in binary or ternary combinations on css. surface treatments incorporating nis (25000 iu/ml) ...201425089272
development of double loop-mediated isothermal amplification to detect listeria monocytogenes in food.in this study, a double loop-mediated isothermal amplification (dlamp) based on two target genes hlya and iap was developed for the rapid detection of listeria monocytogenes in food. the results revealed that the detection time and temperature of our dlamp assay for l. monocytogenes were 15 min and 63 °c respectively, with a sensitivity of 10 fg dna of l. monocytogenes per tube. while normal lamp (nlamp) of hlya or iap was 100 fg dna of l. monocytogenes per tube for 45 min and 63 °c. furthermore ...201425086581
modelling the effect of lactic acid bacteria from starter- and aroma culture on growth of listeria monocytogenes in cottage cheese.four mathematical models were developed and validated for simultaneous growth of mesophilic lactic acid bacteria from added cultures and listeria monocytogenes, during chilled storage of cottage cheese with fresh- or cultured cream dressing. the mathematical models include the effect of temperature, ph, nacl, lactic- and sorbic acid and the interaction between these environmental factors. growth models were developed by combining new and existing cardinal parameter values. subsequently, the refe ...201425086348
diversity and distribution of listeria monocytogenes in meat processing plants.listeria monocytogenes is a major concern for the meat processing industry because many listeriosis outbreaks have been linked to meat product consumption. the aim of this study was to elucidate l. monocytogenes diversity and distribution across different spanish meat processing plants. l. monocytogenes isolates (n = 106) collected from food contact surfaces of meat processing plants and meat products were serotyped and then characterised by multilocus sequence typing (mlst). the isolates were s ...201425084653
efficacy of plant-derived compounds combined with hydrogen peroxide as antimicrobial wash and coating treatment for reducing listeria monocytogenes on cantaloupes.the efficacy of four plant-derived antimicrobials (pdas), namely carvacrol, thymol, β-resorcylic acid, and caprylic acid, with or without hydrogen peroxide (hp), as antimicrobial wash and chitosan based coating for reducing listeria monocytogenes (lm) on cantaloupes was investigated. cantaloupe rind plugs inoculated with lm (10(7) cfu/cm(2)) were washed for 3, 6, 10 min at 25 °c or 1, 3, 5 min at 55 or 65 °c in water, or water containing 2% pdas with or without 2% hp. additionally, inoculated ca ...201425084644
radiation therapy combined with listeria monocytogenes-based cancer vaccine synergize to enhance tumor control in the b16 melanoma model.conceptually, the immune system may profoundly influence the efficacy of radiation therapy. compelling evidence has recently emerged revealing the capacity of local radiation therapy (rt) to induce antitumor immune responses and sparked interest in combining rt with immunotherapy to promote tumor-specific immunity. a listeria monocytogenes (lm)-based cancer vaccine engineered to express tumor-associated antigen has been shown to effectively retard tumor growth by cell-mediated immune mechanisms. ...201425083327
attenuated listeria monocytogenes reprograms m2-polarized tumor-associated macrophages in ovarian cancer leading to inos-mediated tumor cell lysis.a principal mechanism by which tumors evade immune-mediated elimination is through immunosuppression. previous approaches to tumor immunotherapy have focused on modifying the immunosuppressive environment with immune checkpoint inhibitors, cytokine therapy, and other modalities with the intent to generate t-cell based anti-tumor immunity. we hypothesized that transformation of the suppressive ovarian cancer microenvironment could be achieved by introduction of the attenuated δacta/δinlb strain o ...201425083323
the rab5-gef function of rin1 regulates multiple steps during listeria monocytogenes infection.listeria monocytogenes is a food-borne pathogenic bacterium that invades intestinal epithelial cells through a phagocytic pathway that relies on the activation of host cell rab5 gtpases. listeria monocytogenes must subsequently inhibit rab5, however, in order to escape lysosome-mediated destruction. relatively little is known about upstream rab5 regulators during l. monocytogenes entry and phagosome escape processes in epithelial cells. here we identify rin1, a ras effector and rab5-directed gua ...201425082076
phylogenetic analysis of the listeria monocytogenes based on sequencing of 16s rrna and hlya genes.the discrimination between listeria monocytogenes and listeria species has been detected. the 16s rrna and hlya were pcr amplified with set of oligonucleotide primers with flank 1,500 and 456 bp fragments, respectively. based on the differences in 16s rrna and hlya genes, a total 80 isolates from different environmental, food and clinical samples confirmed it to be l. monocytogenes. the 16s rrna sequence similarity suggested that the isolates were similar to the previously reported ones from dif ...201425205124
efficacy of acetic acid against listeria monocytogenes attached to poultry skin during refrigerated storage.this work evaluates the effect of acetic acid dipping on the growth of l. monocytogenes on poultry legs stored at 4 °c for eight days. fresh inoculated chicken legs were dipped into either a 1% or 2% acetic acid solution (v/v) or distilled water (control). changes in mesophiles, psychrotrophs, enterobacteriaceae counts and sensorial characteristics (odor, color, texture and overall appearance) were also evaluated. the shelf life of the samples washed with acetic acid was extended by at least two ...201428234335
summary of 11 years of enteric outbreak investigations and criteria to initiate an investigation, province of quebec, 2002 through 2012.this article presents a retrospective analysis of enteric disease outbreak investigations led by or conducted in collaboration with provincial health authorities in the province of quebec from 2002 through 2012. objectives were to characterize enteric disease outbreaks, quantify and describe those for which a source was identified (including the control measures implemented), identify factors that contributed to or impeded identification of the source, and recommend areas for improvement in outb ...201425198848
listeria monocytogenes serotype 1/2b and 4b isolates from human clinical cases and foods show differences in tolerance to refrigeration and salt stress.control of listeria monocytogenes in food processing facilities is a difficult issue because of the ability of this microorganism to form biofilms and adapt to adverse environmental conditions. survival at high concentrations of sodium chloride and growth at refrigeration temperatures are two other important characteristics of l. monocytogenes isolates. the aim of this study was to compare the growth characteristics under stress conditions at different temperatures of l. monocytogenes serotypes ...201425198843
thermal and nonthermal factors affecting survival of salmonella and listeria monocytogenes in animal manure-based compost mixtures.reduction of enteric pathogens in animal manures before field application is essential for mitigating the risk of foodborne illness associated with produce. aerobic composting of manures has been advocated as an effective treatment for reducing pathogen populations, and heat is a major factor contributing to pathogen inactivation. this study was initiated to determine the potential contribution of both thermal and nonthermal (ph, volatile acids, and ammonia) factors to pathogen inactivation duri ...201425198842
prevalence and antimicrobial susceptibility of listeria monocytogenes on chicken carcasses in bandung, indonesia.this study was conducted to determine the prevalence and quantify the number of listeria monocytogenes in fresh chicken carcasses sold in traditional markets and supermarkets in bandung, west java, indonesia, and to determine the antimicrobial resistance patterns of the isolated l. monocytogenes strains. the overall prevalence of l. monocytogenes in chicken carcasses was 15.8% (29/184). when comparing samples from traditional markets and supermarkets, no significant difference in the l. monocyto ...201425198605
comparison of listeria monocytogenes isolates across the island of ireland.building a comprehensive knowledge base of the association of listeria monocytogenes isolates across national food chains, clinical cases, and environments can play a key role in helping control the incidence of listeriosis. today, many food chains cross national borders and are often shared by neighboring countries. this study characterized l. monocytogenes isolated from food samples in northern ireland and investigated whether similarities in the population and associations of l. monocytogenes ...201425198604
low, medium, and high heat tolerant strains of listeria monocytogenes and increased heat stress resistance after exposure to sublethal heat.a group of 37 strains representing all 13 serotypes of listeria monocytogenes with an initial cell density of 10(7) cfu/ml were analyzed for their heat tolerance at 60°c for 10 min. these l. monocytogenes strains were categorized into three heat tolerance groups: low (<2 log cfu/ml survival), medium (2 to 4 log cfu/ml survival), and high (4 to 6 log cfu/ml survival). serotype 1/2a strains had relatively low heat tolerance; seven of the eight tested strains were classified as low heat tolerant. o ...201425198590
growth of listeria monocytogenes, salmonella spp., escherichia coli o157:h7, and staphylococcus aureus on cheese during extended storage at 25°c.potentially hazardous foods require time/temperature control for safety. according to the u.s. food and drug administration food code, most cheeses are potentially hazardous foods based on ph and water activity, and a product assessment is required to evaluate safety of storage >6 h at 21°c. we tested the ability of 67 market cheeses to support growth of listeria monocytogenes (lm), salmonella spp. (salm), escherichia coli o157:h7 (ec), and staphylococcus aureus (sa) over 15 days at 25°c. hard ( ...201425198588
virulence and genotypic characterization of listeria monocytogenes isolated from vegetable and soil samples.listeria monocytogenes, a foodborne pathogen is ubiquitous to different environments including the agroecosystem. the organism poses serious public health problem. therefore, an attempt has been made to gain further insight to their antibiotic susceptibility, serotypes and the virulence genes.201425195727
monitoring occurrence and persistence of listeria monocytogenes in foods and food processing environments in the republic of ireland.although rates of listeriosis are low in comparison to other foodborne pathogenic illness, listeriosis poses a significant risk to human health as the invasive form can have a mortality rate as high as 30%. food processors, especially those who produce ready-to-eat (rte) products, need to be vigilant against listeria monocytogenes, the causative pathogen of listeriosis, and as such, the occurrence of l. monocytogenes in food and in the food processing environment needs to be carefully monitored. ...201425191314
potential of cell-free supernatants from cultures of selected lactic acid bacteria and yeast obtained from local fermented foods as inhibitors of listeria monocytogenes, salmonella spp. and staphylococcus aureus.food-borne infections cause huge economic and human life losses worldwide. the most common contaminants of foods include listeria monocytogenes salmonellae and staphylococcus aureus. l. monocytogenes is most notorious due to its tolerance to common food preservation methods and the risks it poses, including higher fatality rates. safer, more efficacious control methods are thus needed. along with food-borne pathogens, lactic acid bacteria (lab) can also be found in foods. some lab isolates inhib ...201425190588
systemic c3 modulates cd8+ t cell contraction after listeria monocytogenes infection.ag-specific cd8(+) t cell contraction (contraction), which occurs after the resolution of infection, is critical for homeostasis of the immune system. although complement components regulate the primary cd8(+) t cell response, there is insufficient evidence supporting their role in regulating contraction and memory. in this study, we show that c3-deficient (c3(-/-)) mice exhibited significantly less cd8(+) t cell contraction than did wild-type mice postinfection with recombinant listeria monocyt ...201425187659
[host responses to bacterial infections].pathogenic bacteria and host defense system have been evolved by their offense and defense. in vivo research is crucial for elucidation of interactions between them. i have investigated their offence and defense by various standpoints using mouse models of listeria monocytogenes and staphylococcus aureus infections. herein, the results of my research including the roles of endogenous cytokines in host defense, the attenuation of host defense mechanism in obesity and diabetes, the development of ...201425186639
an educationally inspired illustration of two-dimensional quantitative microbiological risk assessment (qmra) and sensitivity analysis.quantitative microbiological risk assessment (qmra) is a structured methodology used to assess the risk involved by ingestion of a pathogen. it applies mathematical models combined with an accurate exploitation of data sets, represented by distributions and - in the case of two-dimensional monte carlo simulations - their hyperparameters. this research aims to highlight background information, assumptions and truncations of a two-dimensional qmra and advanced sensitivity analysis. we believe that ...201425173917
benzalkonium chloride and heavy-metal tolerance in listeria monocytogenes from retail foods.phenotypic and genotypic tolerance in 71 listeria monocytogenes isolates from different varieties of foods to benzalkonium chloride (bc) and cadmium were investigated by susceptibility test and molecular methods. to investigate the role of efflux pumps in bc tolerance, reserpine, an efflux pump inhibitor, was added to the bc tolerant strains. tolerance to bc and cadmium were 26.8% (19/71) and 49.3% (35/71) respectively. strains with bc tolerance were significantly more frequent among those of se ...201425173916
Refresh
firstprev
...21222324252627282930...
nextlast
Displaying items 2401 - 2500 of 16792