Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| competitive exclusion as a mode of action of a novel bacillus cereus aquaculture biological agent. | to determine the contribution of potential modes of action of a bacillus cereus aquaculture biological control agent in inhibition of the fish pathogen, aeromonas hydrophila. | 2010 | 20337929 |
| comparative transcriptomic and phenotypic analysis of the responses of bacillus cereus to various disinfectant treatments. | antimicrobial chemicals are widely applied to clean and disinfect food-contacting surfaces. however, the cellular response of bacteria to various disinfectants is unclear. in this study, the physiological and genome-wide transcriptional responses of bacillus cereus atcc 14579 exposed to four different disinfectants (benzalkonium chloride, sodium hypochlorite, hydrogen peroxide, and peracetic acid) were analyzed. for each disinfectant, concentrations leading to the attenuation of growth, growth a ... | 2010 | 20348290 |
| peptides panned from a phage-displayed random peptide library are useful for the detection of bacillus anthracis surrogates b. cereus 4342 and b. anthracis sterne. | recent use of bacillus anthracis as a bioweapon has highlighted the need for a sensitive monitoring system. current bacterial detection tests use antibodies as bio-molecular recognition elements which have limitations with regard to time, specificity and sensitivity, creating the need for new and improved cost-effective high-affinity detection probes. in this study, we screened a commercially available bacteriophage-displayed random peptide library using bacillus cereus 4342 cells as bait to ide ... | 2010 | 20350526 |
| fulminant sepsis caused by bacillus cereus in patients with hematologic malignancies: analysis of its prognosis and risk factors. | bacillus cereus is a growing concern as a cause of life-threatening infections in patients with hematologic malignancies. however, the risk factors for patients with unfavorable outcomes have not been fully elucidated. at our institution, we observed the growth of b. cereus in blood culture in 68 patients with (23) or without (45) hematologic malignancies treated from september 2002 to november 2009. we defined a case as having sepsis when more than two blood culture sets were positive for b. ce ... | 2010 | 20367571 |
| expanding the known repertoire of virulence factors produced by bacillus cereus through early secretome profiling in three redox conditions. | the pathogen bacillus cereus causes diarrheal disease in humans. in the small intestine, b. cereus has to deal with anaerobiosis, low oxidoreduction potential, and carbohydrate limitation conditions. to gain insight into the virulence potential of low density b. cereus cells in such an environment, we cultured bacteria in low and high oxidoreduction potential anoxic conditions and in fully oxic conditions and compared their full secretomes. a unique pattern of proteins assigned to virulence fact ... | 2010 | 20368289 |
| removal of sediment and bacteria from water using green chemistry. | although nearly all newly derived water purification methods have improved the water quality in developing countries, few have been accepted and maintained for long-term use. field studies indicate that the most beneficial methods use indigenous resources, as they are both accessible and accepted by communities they help. in an effort to implement a material that will meet community needs, two fractions of mucilage gum were extracted from the opuntia ficus-indica cactus and tested as flocculatio ... | 2010 | 20369814 |
| characterization of bacterial spore germination using integrated phase contrast microscopy, raman spectroscopy, and optical tweezers. | we present a methodology that combines external phase contrast microscopy, raman spectroscopy, and optical tweezers to monitor a variety of changes during the germination of single bacillus cereus spores in both nutrient (l-alanine) and non-nutrient (ca-dipicolinic acid (dpa)) germinants with a temporal resolution of approximately 2 s. phase contrast microscopy assesses changes in refractility of individual spores during germination, while raman spectroscopy gives information on changes in spore ... | 2010 | 20369827 |
| insertional mutagenesis reveals genes involved in bacillus cereus atcc 14579 growth at low temperature. | transposon mutagenesis of bacillus cereus atcc 14579 yielded cold-sensitive mutants. mutants of genes encoding enzymes of the central metabolism were affected by cold, but also by other stresses, such as ph or salt, whereas a mutant with transposon insertion in the promoter region of bc0259 gene, encoding a putative dead-box rna helicase displaying homology with escherichia coli csda and bacillus subtilis csha rna helicases, was only cold-sensitive. expression of the bc0259 gene at 10 degrees c ... | 2010 | 20370835 |
| phytochemical and biological investigations of elaeodendron schlechteranum. | elaeodendron schlechteranum (loes.) loes. is a shrub or tree belonging to the family celastraceae. in tanzania, in addition to ethnopharmacological claims in treating various non-infectious diseases, the root and stem bark powder is applied on septic wounds, and the leaf paste is used for treatment of boils and carbuncles. the aim of this study was to identify the putative active constituents of the plant. | 2010 | 20371284 |
| bacillus cereus, a volatile human pathogen. | bacillus cereus is a gram-positive aerobic or facultatively anaerobic, motile, spore-forming, rod-shaped bacterium that is widely distributed environmentally. while b. cereus is associated mainly with food poisoning, it is being increasingly reported to be a cause of serious and potentially fatal non-gastrointestinal-tract infections. the pathogenicity of b. cereus, whether intestinal or nonintestinal, is intimately associated with the production of tissue-destructive exoenzymes. among these sec ... | 2010 | 20375358 |
| rapid degradation of n-3-oxo-acylhomoserine lactones by a bacillus cereus isolate from malaysian rainforest soil. | a bacterial strain, km1s, was isolated from a malaysian rainforest soil sample by using a defined enrichment medium that specifically facilitates selection of quorum quenching bacteria. km1s was clustered closely to bacillus cereus by 16s ribosomal dna sequence analysis. it degraded n-3-oxo-hexanoyl homoserine lactone and n-3-oxo-octanoyl homoserine lactone in vitro rapidly at 4.98 and 6.56 microg ahl h(-1) per 10(9) cfu/ml, respectively, as determined by the rapid resolution liquid chromatograp ... | 2010 | 20376561 |
| efficient amplification of genes involved in microbial secondary metabolism by an improved genome walking method. | genome walking is a commonly used technique for the identification of dna sequences adjacent to known regions. despite the development of various genome walking methods, nonspecific products are often produced in certain circumstances, especially when gc-rich dna sequences are dealt with. to effectively resolve such technical issues, a simple nested polymerase chain reaction-based genome walking method has been developed by implementing a progressively decreased annealing temperature from 70 deg ... | 2010 | 20376630 |
| detoxification and discoloration of moroccan olive mill wastewater by electrocoagulation. | the objective of the present study was to assess the electrocoagulation treatment of olive mill wastewater using an aluminum electrode. we have examined the effect of the following parameters on the removal of chemical oxygen demand (cod), polyphenols and dark color removal efficiency: electrolysis time, current density, chloride concentration and initial ph. the olive mill wastewater (omw)--diluted 5 times--used in this study had 20.000 mg/l chemical oxygen demand, 3.6 ms/cm conductivity and ac ... | 2010 | 19880250 |
| the silicon layer supports acid resistance of bacillus cereus spores. | silicon (si) is considered to be a "quasiessential" element for most living organisms. however, silicate uptake in bacteria and its physiological functions have remained obscure. we observed that si is deposited in a spore coat layer of nanometer-sized particles in bacillus cereus and that the si layer enhances acid resistance. the novel acid resistance of the spore mediated by si encapsulation was also observed in other bacillus strains, representing a general adaptation enhancing survival unde ... | 2010 | 19880606 |
| e. coli, p. aeruginosa, and b. cereus bacteria sterilization using afterglow of non-thermal plasma at atmospheric pressure. | we developed and employed a new geometrical structure of dielectric barrier discharge in atmospheric pressure for bacterial broad spectrum sterilization. we utilized a plasma source having an ac power supply at 50 hz and 5,400 v (rms value). we prepared suspensions of the gram-negative bacteria species (escherichia coli, pseudomonas aeruginosa) and a gram-positive of bacillus cereus with luria-bertani broth media up to od(600 nm) = 0.25 of mcfarland standard. afterglow of non-thermal atmospheric ... | 2010 | 19882114 |
| selective adhesion of bacillus cereus spores on heterogeneously wetted silicon nanowires. | the article reports on the selective adhesion of bacillus cereus spores on patterned and heterogeneously wetted superhydrophobic silicon nanowires surfaces. superhydrophilic patterns on superhydrophobic silicon nanowire (sinw) surfaces were prepared by a standard optical lithography technique. exposure of the patterned surface to a suspension of b. cereus spores in water led to their specific adsorption in superhydrophobic areas. comparable results were obtained on a patterned hydrophobic/hydrop ... | 2010 | 19891454 |
| biodegradation of polyacrylamide by bacteria isolated from activated sludge and oil-contaminated soil. | polyacrylamide (pam), a linear water soluble polymeric compound with high molecular weight, is extensively used for oil production in china. compared with the physico-chemical degradation of pam, there is no acrylamide monomer, which causes peripheral neuropathy, released in the process of biodegradation. unfortunately, few microorganisms have been isolated which can degrade pam. in this study, two pam-degrading bacterial strains, named hwbi and hwbii, were isolated from the activated sludge and ... | 2010 | 19932560 |
| bacillus thuringiensis serovars bolivia, vazensis and navarrensis meet the description of bacillus weihenstephanensis. | the bacillus cereus sensu lato group comprises six related species: b. cereus, b. anthracis, b. thuringiensis, b. mycoides, b. pseudomycoides and b. weihenstephanensis. bacillus thuringiensis is a mesophile. it is distinguished from other members of the b. cereus group by the apparition of an inclusion body upon sporulation. b. weihenstephanensis, however, is a psychrotolerant. it grows at 7 degrees c but not at 43 degrees c. it is further characterised by the presence of specific signature sequ ... | 2010 | 19937033 |
| discrimination and phylogenomic classification of bacillus anthracis-cereus-thuringiensis strains based on lc-ms/ms analysis of whole cell protein digests. | modern taxonomy, diagnostics, and forensics of bacteria benefit from technologies that provide data for genome-based classification and identification of strains; however, full genome sequencing is still costly, lengthy, and labor intensive. therefore, other methods are needed to estimate genomic relatedness among strains in an economical and timely manner. although dna-dna hybridization and techniques based on genome fingerprinting or sequencing selected genes like 16s rdna, gyrb, or rpob are f ... | 2010 | 19938824 |
| synthesis of nitroimidazole derived oxazolidinones as antibacterial agents. | a series of n-alkylated derivatives of nitroimidazolyl oxazolidinones 6a-i with various substituent at n-1 position of the nitroimidazole were synthesized and their in-vitro antibacterial activities were evaluated against several gram-positive and gram-negative bacteria. the 6a was found to be most potent compound in the series with mic at 0.097 microg/ml against bacillus cereus mtcc 430.). both 6a and 6f did not exhibit any toxicity towards mammalian cell l929. | 2010 | 19942324 |
| toxinogenic and spoilage potential of aerobic spore-formers isolated from raw milk. | the harmful effects on the quality and safety of dairy products caused by aerobic spore-forming isolates obtained from raw milk were characterized. quantitative assessment showed strains of bacillus subtilis, the bacillus cereus group, paenibacillus polymyxa and bacillus amyloliquefaciens to be strongly proteolytic, along with bacillus licheniformis, bacillus pumilus and lysinibacillus fusiformis to a lesser extent. lipolytic activity could be demonstrated in strains of b. subtilis, b. pumilus a ... | 2010 | 19944473 |
| detection of bacillus cereus with enteropathogenic potential by multiplex real-time pcr based on sybr green i. | in order to meet the growing demand for fast and reliable detection of potentially toxinogenic bacillus cereus, we developed a multiplex real-time pcr assay based on sybr green i with subsequent melting curve analysis. we designed and selected primers specific for genes of toxins responsible for diarrhoea (nhea, hbld and cytk1) and emesis (ces). a panel of 337 bacillus strains was applied to the novel method on light cycler 2.0 with average melting temperature (t(m)) values of 73.85 degrees c (n ... | 2010 | 19944752 |
| fnr mediates carbohydrate-dependent regulation of catabolic and enterotoxin genes in bacillus cereus f4430/73. | we had previously demonstrated that fnr is required for fermentative growth and oxic production of hemolysin bl (hbl) and non-hemolytic enterotoxin (nhe) in the food-borne pathogen bacillus cereus f4430/73. in the present work, the regulatory impact of fnr on microaerobic growth and enterotoxin production in response to carbohydrates was examined using glucose, fructose, sucrose or a glucose-fructose mixture as carbon and energy sources. growth parameters, byproduct spectra and transcription lev ... | 2010 | 19944753 |
| isolation and characterization of hyper phenol tolerant bacillus sp. from oil refinery and exploration sites. | bacillus cereus mtcc 9817 strain akg1 and b. cereus mtcc 9818 strain akg2 were isolated from petroleum refinery and oil exploration site, respectively. the 16s rdna sequence of strain akg1 showed the closest relation to b. cereus 99.63% and bacillus coagulans 99.63% followed by 99.34% homology with bacillus thuringiensis strain 2pr56-10. akg2 is mostly related to b. thuringiensis strain cmg 861 with 99.37% homology. the similarity search between akg1 and akg2 gave the lowest similarity 99.19% am ... | 2010 | 19945784 |
| synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth. | we have previously described a murein hydrolase activity for the surface layer (s-layer) of lactobacillus acidophilus atcc 4356. here we show that, in combination with nisin, this s-layer acts synergistically to inhibit the growth of pathogenic gram-negative salmonella enterica and potential pathogenic gram-positive bacteria, staphylococcus aureus and bacillus cereus. in addition, bacteriolytic effects were observed for the gram-positive species tested. we postulate that the s-layer enhances the ... | 2010 | 19948852 |
| application of the bactericidal activity of epsilon-poly-l-lysine to the storage of human platelet concentrates. | epsilon-poly-l-lysine (epsilon-pll) is a polypeptide comprising approximately 30 l-lysine subunits generated by bond formation between alpha-carboxy and epsilon-amino groups. it is an approved antimicrobial food preservative in japan. however, the efficacy of epsilon-pll as an antibacterial additive for storage of human platelet concentrates (pcs) is not known. | 2010 | 19951313 |
| extremely long time stability study of selected antibiotic standards. | a study was conducted to investigate the quality of reference substances which were produced long before the introduction of advanced analysis and purification techniques. the quality and antimicrobial activity of who and ep oxytetracycline, doxycycline, colistin and spiramycin reference standards were assessed. the oldest substance was stored for 54 years in a freezer. assay and purity tests were conducted according to ph. eur. 6.0. additionally, antibacterial activity was tested with the micro ... | 2010 | 19850430 |
| fatal spontaneous bacterial peritonitis and necrotizing fasciitis with bacteraemia caused by bacillus cereus in a patient with cirrhosis. | we report a case of spontaneous bacterial peritonitis and necrotizing fasciitis caused by bacillus cereus in a cirrhotic patient without preceding disruption of skin or symptoms of gastroenteritis. this rapidly fatal infection due to b. cereus adds to the long list of aetiologies of infectious complications among patients with cirrhosis of the liver. | 2010 | 19850708 |
| a downstream process for production of a viable and stable bacillus cereus aquaculture biological agent. | biological products offer advantages over chemotherapeutics in aquaculture. adoption in commercial application is lacking due to limitations in process and product development that address key end user product requirements such as cost, efficacy, shelf life and convenience. in previous studies, we have reported on the efficacy, physiological robustness and low-cost spore production of a bacillus cereus isolate (nrrl 100132). this study examines the development of suitable spore recovery, drying, ... | 2010 | 19921182 |
| new antibacterial and cytotoxic activities of falcarindiol isolated in crithmum maritimum l. leaf extract. | bioassay-guided fractionation of a chloroformic extract obtained from crithmum maritimum leaves led to the chemical isolation of falcarindiol, a polyacetylene widely distributed within the apiaceae family. structure of this compound was confirmed by nmr and activity was tracked using a screening microplate bioassay. falcarindiol strongly inhibited the growth of micrococcus luteus and bacillus cereus, with a minimum inhibitory concentration (mic) value of 50 microg ml(-1). moreover, this compound ... | 2010 | 19922760 |
| a novel hybrid kinase is essential for regulating the sigma(b)-mediated stress response of bacillus cereus. | a common bacterial strategy for monitoring environmental challenges is to use two-component systems, which consist of a sensor histidine kinase (hk) and a response regulator (rr). in the food-borne pathogen bacillus cereus, the alternative sigma factor sigma(b) is activated by the rr rsby. here we present strong indications that the pp2c-type phosphatase rsby receives its input from the multi-sensor hybrid kinase bc1008 (renamed rsbk). genome analyses revealed that, across bacilli, rsby and rsbk ... | 2010 | 19958380 |
| extraction and characterization of chitin, chitosan, and protein hydrolysates prepared from shrimp waste by treatment with crude protease from bacillus cereus sv1. | chitin is a polysaccharide found in abundance in the shell of crustaceans. in this study, the protease from bacillus cereus sv1 was applied for chitin extraction from shrimp waste material of metapenaeus monoceros. a high level of deproteinization 88.8% +/- 0.4 was recorded with an e/s ratio of 20. the demineralization was completely achieved within 6 h at room temperature in hcl 1.25 m, and the residual content of calcium in chitin was below 0.01%. (13)c cp/mas-nmr spectral analysis of chitin p ... | 2010 | 19960271 |
| arbuscular mycorrhizal fungi, bacillus cereus, and candida parapsilosis from a multicontaminated soil alleviate metal toxicity in plants. | we investigated if the limited development of trifolium repens growing in a heavy metal (hm) multicontaminated soil was increased by selected native microorganisms, bacteria (bacillus cereus (bc)), yeast (candida parapsilosis (cp)), or arbuscular mycorrhizal fungi (amf), used either as single or dual inoculants. these microbial inoculants were assayed to ascertain whether the selection of hm-tolerant microorganisms can benefit plant growth and nutrient uptake and depress hm acquisition. the inoc ... | 2010 | 20013261 |
| primary multiple cerebral invasion in acute promyelocytic leukemia dramatically worsened by all-trans retinoic acid. | 2010 | 20015546 | |
| direct-imaging-based quantification of bacillus cereus atcc 14579 population heterogeneity at a low incubation temperature. | bacillus cereus atcc 14579 was cultured in microcolonies on anopore strips near its minimum growth temperature to directly image and quantify its population heterogeneity at an abusive refrigeration temperature. eleven percent of the microcolonies failed to grow during low-temperature incubation, and this cold-induced population heterogeneity could be partly attributed to the loss of membrane integrity of individual cells. | 2010 | 20023091 |
| physical characteristics of spores of food-associated isolates of the bacillus cereus group. | all 47 food-borne isolates of bacillus cereus sensu stricto, as well as 10 of 12 food-borne, enterotoxigenic isolates of bacillus thuringiensis, possessed appendages. spores were moderately to highly hydrophobic, and each had a net negative charge. these characteristics indicate that spores of food-associated b. thuringiensis and not only b. cereus sensu stricto have high potential to adhere to inert surfaces. | 2010 | 20023097 |
| characterisation and toxicity of bacillus thuringiensis strains from hazelnut pests and fields. | in order to find and identify more toxic insecticidal bacillus thuringiensis berliner (bt) strains, a survey was carried out of b. thuringiensis isolate pests belonging to coleoptera, lepidoptera and diptera and from soils in hazelnut fields. of 16 isolates having bacillus cereus-b. thuringiensis morphology, eight were classified as b. thuringiensis because of the production of parasporal delta-endotoxin crystals. | 2010 | 20024949 |
| involvement of motility and flagella in bacillus cereus biofilm formation. | bacillus cereus is a food-borne pathogen and a frequent contaminant of food production plants. the persistence of this pathogen in various environments results from the formation of spores and of biofilms. to investigate the role of the b. cereus flagellar apparatus in biofilm formation, we constructed a non-flagellated mutant and a flagellated but non-motile mutant. unexpectedly, we found that the presence of flagella decreased the adhesion of the bacterium to glass surfaces. we hypothesize tha ... | 2010 | 20035003 |
| dissecting interactions between nucleosides and germination receptors in bacillus cereus 569 spores. | bacillus cereus 569 spores germinate either with inosine as a sole germinant or with a combination of nucleosides and l-alanine. whereas the inosine-only germination pathway requires the presence of two different germination receptors (geri and gerq) to be activated, the nucleoside/alanine germination pathway only needs one of the two receptors. to differentiate how nucleoside recognition varies between the inosine-only germination pathway and the nucleoside/alanine germination pathway, we teste ... | 2010 | 20035009 |
| occurrence of psychrotolerant bacillus cereus group strains in ice creams. | the occurrences of bacillus cereus group strains in 40 ice cream samples were investigated. among 109 isolated b. cereus group strains confirmed by 16s rdna sequence analysis only 50 were identified as b. cereus and one as b. thuringiensis by using fda (u.s. food and drug administration) standard, indicating the two identification standards were highly inconsistent. furthermore, the psychrotolerant growth properties and the occurrence of specific psychrotolerant genes of the isolates were also s ... | 2010 | 20036024 |
| photodynamic antimicrobial activity of avian eggshell pigments. | pigmentation in avian eggshells appears to be associated with shell strength, temperature regulation, and camouflage. the pigments found in eggshells are mainly porphyrins, which have been utilized therapeutically as photosensitizers. here, we examined the photoinactivation of gram-positive (staphylococcus aureus, bacillus cereus) and gram-negative bacteria (escherichia coli, salmonella enteritidis) by hen eggshells and their pigments. the results indicated that eggshells have a light-dependent ... | 2010 | 20036664 |
| identification of the main promoter directing cereulide biosynthesis in emetic bacillus cereus and its application for real-time monitoring of ces gene expression in foods. | cereulide, the emetic bacillus cereus toxin, is synthesized by cereulide synthetase via a nonribosomal peptide synthetase (nrps) mechanism. previous studies focused on the identification, structural organization, and biochemical characterization of the ces gene locus encoding cereulide synthetase; however, detailed information about the transcriptional organization of the ces genes was lacking. the present study shows that the cesptabcd genes are transcribed as a 23-kb polycistronic transcript, ... | 2010 | 20038713 |
| production, characterization, and antimicrobial activity of a bacteriocin from newly isolated enterococcus faecium ij-31. | this work aimed to isolate and characterize enterococcus spp. from indigenous dairy products in islamabad, pakistan. by classical microbiological techniques, one strain from a butter sample was identified to be enterococcus faecium, and we designated it e. faecium ij-31. the precise identity of this strain was then established by determining the sequence of its 16s and 23s rrna genes. the sequence homology searches revealed matches with a number of previously reported strains, such as e. faecium ... | 2010 | 20051203 |
| multi-functional p(3hb) microsphere/45s5 bioglass-based composite scaffolds for bone tissue engineering. | novel multi-functional p(3hb) microsphere/45s5 bioglass-based composite scaffolds exhibiting potential for drug delivery were developed for bone tissue engineering. 45s5 bioglass-based glass-ceramic scaffolds of high interconnected porosity produced using the foam-replication technique were coated with biodegradable microspheres (size<2 microm) made from poly(3-hydroxybutyrate), p(3hb), produced using bacillus cereus spv. a solid-in-oil-in-water emulsion solvent extraction/evaporation technique ... | 2010 | 20056174 |
| analysis of acid-stressed bacillus cereus reveals a major oxidative response and inactivation-associated radical formation. | acid stress resistance of the food-borne human pathogen bacillus cereus may contribute to its survival in acidic environments, such as encountered in soil, food and the human gastrointestinal tract. the acid stress responses of b. cereus strains atcc 14579 and atcc 10987 were analysed in aerobically grown cultures acidified to ph values ranging from ph 5.4 to ph 4.4 with hcl. comparative phenotype and transcriptome analyses revealed three acid stress-induced responses in this ph range: growth ra ... | 2010 | 20074238 |
| comparison of two optical-density-based methods and a plate count method for estimation of growth parameters of bacillus cereus. | quantitative microbiological models predicting proliferation of microorganisms relevant for food safety and/or food stability are useful tools to limit the need for generation of biological data through challenge testing and shelf-life testing. the use of these models requires quick and reliable methods for the generation of growth data and estimation of growth parameters. growth parameter estimation can be achieved using methods based on plate counting and methods based on measuring the optical ... | 2010 | 20081006 |
| isolation, structure elucidation and biological activity of angucycline antibiotics from an epiphytic yew streptomycete. | in the course of study of epiphytic microorganisms occurring on the surface of roots of taxus baccata l. a new strain streptomyces sp. ac113 was isolated. according to 16s ribosomal dna-based identification the new strain is 99% identical with streptomyces flavidofuscus. this strain cultivated in an arginine glycerol medium produced three major metabolites identified as (-)-8-o -methyltetrangomycin (1), 8-o -methyltetrangulol (2) and 8-o -methyl-7-deoxo-7-hydroxytetrangomycin (3). the chemical s ... | 2010 | 20082376 |
| safety assessment of recalled haemophilus influenzae type b (hib) conjugate vaccines--united states, 2007-2008. | on 13 december 2007, merck & co., inc. voluntarily recalled 1.2 million doses of haemophilus influenzae type b (hib) vaccines that had been distributed since april 2007 for concerns regarding potential bacillus cereus contamination. enhanced postrecall surveillance was conducted to detect vaccine-associated b. cereus infections. | 2010 | 20084617 |
| hydrolysis of amylopectin by amylolytic enzymes: level of inner chain attack as an important analytical differentiation criterion. | differences in amylase action pattern on amylopectin were demonstrated by the relation between the decrease in potassium iodide-iodine binding of waxy maize starch and the increase in reducing value during hydrolysis, as expressed by the rv(80) value (i.e., the reducing value for a potassium iodide-iodine binding value of 80% of that of the starting material). in the initial stages of the hydrolysis, the ratio of the increase in the level of reducing polysaccharides to the increase in the total ... | 2010 | 19962134 |
| development of a stable isotope dilution analysis for the quantification of the bacillus cereus toxin cereulide in foods. | an increasing number of severe food borne intoxications are caused by a highly stable depsipeptide, named cereulide, which is produced by emetic bacillus cereus strains. as cereulide poses a health risk to humans, the development of an appropriate method for the analysis of this toxin is mandatory. therefore, the reference material of cereulide as well as its (13)c(6)-isotopologue was prepared by means of a biosynthetic approach using a b. cereus culture, followed by a rapid but efficient downst ... | 2010 | 19994891 |
| a pilot study of the microbiological quality of culturally diverse, ready-to-eat foods from selected retail establishments in melbourne, australia. | in recent years, there has been an increasing number of foodborne outbreaks linked to the consumption of culturally diverse foods. this appears to be because of the increasing quantity of culturally diverse foods available and a preference to store these foods, some of which are considered potentially hazardous, at ambient temperature. this practice may contravene temperature requirements defined by the food standards code. a lack of understanding of the hazardous nature of some culturally prepa ... | 2010 | 20001329 |
| soya bean tempe extracts show antibacterial activity against bacillus cereus cells and spores. | tempe, a rhizopus ssp.-fermented soya bean food product, was investigated for bacteriostatic and/or bactericidal effects against cells and spores of the food-borne pathogen bacillus cereus. | 2010 | 20002864 |
| single chain variable fragment antibodies selected by phage display against the sporozoite surface antigen s16 of cryptosporidium parvum. | two human single chain variable fragment (scfv) libraries were used to select clones that bound to the surface glycoprotein s16 of cryptosporidium parvum. panning of the tomlinson libraries i and j resulted in the isolation of nine distinct clones. of the four clones which had full-length scfv, three contained stop codons. the remaining five clones were truncated, with four missing the heavy chain, and one missing most of the light chain. the full-length clones exhibited better binding to native ... | 2010 | 20093113 |
| use of fatty acid methyl ester profiles for discrimination of bacillus cereus t-strain spores grown on different media. | the goal of this study was to determine if cellular fatty acid methyl ester (fame) profiling could be used to distinguish among spore samples from a single species (bacillus cereus t strain) that were prepared on 10 different medium formulations. to analyze profile differences and identify fame biomarkers diagnostic for the chemical constituents in each sporulation medium, a variety of statistical techniques were used, including nonmetric multidimensional scaling (nmds), analysis of similarities ... | 2010 | 20097814 |
| characterization of wet-heat inactivation of single spores of bacillus species by dual-trap raman spectroscopy and elastic light scattering. | dual-trap laser tweezers raman spectroscopy (ltrs) and elastic light scattering (els) were used to investigate dynamic processes during high-temperature treatment of individual spores of bacillus cereus, bacillus megaterium, and bacillus subtilis in water. major conclusions from these studies included the following. (i) after spores of all three species were added to water at 80 to 90 degrees c, the level of the 1:1 complex of ca(2+) and dipicolinic acid (cadpa; approximately 25% of the dry weig ... | 2010 | 20097820 |
| growth of microorganisms in total parenteral nutrition solutions without lipid. | to identify the microorganisms that can grow rapidly in total parenteral nutrition (tpn) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (staphylococcus aureus, serratia marcescens, bacillus cereus, and candida albicans) in tpn solutions without lipid. | 2010 | 20107529 |
| designed low amphipathic peptides with alpha-helical propensity exhibiting antimicrobial activity via a lipid domain formation mechanism. | although several low amphipathic peptides have been known to exhibit antimicrobial activity, their mode of action has not been completely elucidated. in this study, using designed low amphipathic peptides that retain different alpha-helical content and hydrophobicity, we attempted to investigate the mechanism of these properties. calorimetric and thermodynamic analyses demonstrated that the peptides induce formation of two lipid domains in an anionic liposome at a high peptide-to-lipid ratio. on ... | 2010 | 20109510 |
| inha1, npra, and hlyii as candidates for markers to differentiate pathogenic from nonpathogenic bacillus cereus strains. | bacillus cereus is found in food, soil, and plants, and the ability to cause food-borne diseases and opportunistic infection presumably varies among strains. therefore, measuring harmful toxin production, in addition to the detection of the bacterium itself, may be key for food and hospital safety purposes. all previous studies have focused on the main known virulence factors, cereulide, hbl, nhe, and cytk. we examined whether other virulence factors may be specific to pathogenic strains. inha1, ... | 2010 | 20129969 |
| prevalence of emetic bacillus cereus in different ice creams in bavaria. | in this study, 809 samples of ice cream from different sources were investigated by using cultural methods for the presence of presumptive bacillus cereus. isolates from culture-positive samples were examined with a real-time pcr assay targeting a region of the cereulide synthetase gene (ces) that is highly specific for emetic b. cereus strains. the samples were collected from ice cream parlors and restaurants that produced their own ice cream and from international commercial ice cream companie ... | 2010 | 20132691 |
| antimicrobial activities of tapioca starch/decolorized hsian-tsao leaf gum coatings containing green tea extracts in fruit-based salads, romaine hearts and pork slices. | the antimicrobial activities of edible coatings based on a tapioca starch/decolorized hsian-tsao leaf gum (dhg) matrix with various green tea extracts (gtes) were evaluated. its effect on the shelf-life extension of fruit-based salads, romaine hearts, and pork slices were investigated as well. three types of gtes from hot water (80 degrees c) (w), 40% (e4) and 80% (e8) ethanol were prepared. it was found that all gtes showed pronounced inhibition on gram positive bacteria in agar media, includin ... | 2010 | 20138382 |
| effect of high pressure on the microbiological quality of cooked chicken during storage at normal and abuse refrigeration temperatures. | vacuum-packaged cooked poultry meat was treated at a range of pressures (400-600 mpa) and hold times (1, 2 and 10 min), followed by storage at 4 degrees , 8 degrees or 12 degrees c for up to 35 days. weissella viridescens was found to be the dominant microorganism in the pressure-treated meat, constituting 100% of the microflora identified at 500 and 600 mpa. none of the pressure-treated samples had obvious signs of spoilage during the 35 day storage period, even when the weissella count was >7 ... | 2010 | 20141945 |
| antibacterial activity of carvacrol and 2-nitro-1-propanol against single and mixed populations of foodborne pathogenic bacteria in corn flour dough. | cereal doughs are an important part of human diet, but at the same time can act as vehicles for the transmission of human pathogenic bacteria. in the present study, four pathogenic or toxinogenic bacteria (escherichia coli o157:h7, salmonella enterica serovar enteritidis, bacillus cereus and staphylococcus aureus) were inoculated in a dough made from corn flour in combination with the single antimicrobial compounds carvacrol and 2-nitro-1-propanol (2npoh). survival of single and mixed population ... | 2010 | 20141946 |
| germinant receptor diversity and germination responses of four strains of the bacillus cereus group. | four strains of the bacillus cereus group were compared for their germinant receptor composition and spore germination capacity. phylogenetic analysis of the germinant receptor encoding operons of the enterotoxic strains b. cereus atcc 14579 and atcc 10987, the emetic strain ah187, and the psychrotolerant strain bacillus weihenstephanensis kbab4, indicated a core group of five germinant receptor operons to be present in the four strains, with each strain containing one to three additional recept ... | 2010 | 20153067 |
| coupling of the hydrogen and polyhydroxyalkanoates (pha) production through anaerobic digestion from taihu blue algae. | coupling bio-production of hydrogen and polyhydroxyalkanoates (pha) from taihu blue algae through metabolites circulation was investigated. it was found that the ph adjustment, especially basification was more practical and efficient than other methods for the pretreatment of blue algae before anaerobic digestion. on this occasion, scod, biogas accumulation and hydrogen content reached 26 mg/gts, 500 ml and 37.2%, and which were 4.3, 1.3 and 14.4 times of those of the control group, respectively ... | 2010 | 20153165 |
| the swaposin-like domain of potato aspartic protease (stasp-psi) exerts antimicrobial activity on plant and human pathogens. | plant-specific insert domain (psi) is a region of approximately 100 amino acid residues present in most plant aspartic protease (ap) precursors. psi is not a true saposin domain; it is the exchange of the n- and c-terminal portions of the saposin like domain. hence, psi is called a swaposin domain. here, we report the cloned, heterologous expression and purification of psi from stasp 1 (solanum tuberosum aspartic protease 1), called stasp-psi. results obtained here show that stasp-psi is able to ... | 2010 | 20153392 |
| cytokine profile in a premature infant with systemic bacillus cereus infection. | 2010 | 20158644 | |
| role of the gerp operon in germination and outgrowth of bacillus anthracis spores. | germination of bacillus anthracis spores occurs when nutrients such as amino acids or purine nucleosides stimulate specific germinant receptors located in the spore inner membrane. the gerp(abcdef) operon has been suggested to play a role in facilitating the interaction between germinants and their receptors in spores of bacillus subtilis and bacillus cereus. b. anthracis mutants containing deletions in each of the six genes belonging to the orthologue of the gerp(abcdef) operon, or deletion of ... | 2010 | 20161744 |
| enhancing atp-based bacteria and biofilm detection by enzymatic pyrophosphate regeneration. | the manufacturing processes of many electronic and medical products demand the use of high-quality water. hence the water supply systems for these processes are required to be examined regularly for the presence of microorganisms and microbial biofilms. among commonly used bacteria detection approaches, the atp luminescence assay is a rapid, sensitive, and easy to perform method. the aim of this study is to investigate whether atp regeneration from inorganic pyrophosphate, a product of the atp l ... | 2010 | 20043864 |
| superdormant spores of bacillus species germinate normally with high pressure, peptidoglycan fragments, and bryostatin. | superdormant spores of bacillus cereus and bacillus subtilis germinated just as well as dormant spores with pressures of 150 or 500 mpa and with or without heat activation. superdormant b. subtilis spores also germinated as well as dormant spores with peptidoglycan fragments or bryostatin, a ser/thr protein kinase activator. | 2010 | 20047906 |
| prevalence of bacillus anthracis-like organisms and bacteriophages in the intestinal tract of the earthworm eisenia fetida. | stable infection of bacillus anthracis laboratory strains with environmental bacteriophages confers survival phenotypes in soil and earthworm intestinal niches (r. schuch and v. a. fischetti, plos one 4:e6532, 2009). here, the natural occurrence of two such b. anthracis-infective bacteriophages, wip1 and wip4, was examined in the intestines of eisenia fetida earthworms as part of a 6-year longitudinal study at a pennsylvania forest site. the wip1 tectivirus was initially dominant before being su ... | 2010 | 20118353 |
| synthesis, spectroscopic and biological studies on the new symmetric schiff base derived from 2,6-diformyl-4-methylphenol with n-aminopyrimidine. | a phenol based novel schiff base polydentate symmetric ligand was prepared. the complexes were prepared by reacting ligand and the metal chlorides of cu (ii), ni(ii), co(ii) and fe(ii) in methanol to get a series of mononuclear complexes. the complexes were characterized by elemental analyses, conductivity measurements, magnetic susceptibility data, ir, uv-vis, nmr and api-es mass spectral data. the mononuclear structure of the complexes was confirmed on the basis of elemental analyses, magnetic ... | 2010 | 20163896 |
| evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. | the aim of this study was to evaluate diffusion and dilution methods for determining the antibacterial activity of plant extracts and their mixtures. several methods for measurement of the minimal inhibitory concentration (mic) of a plant extract are available, but there is no standard procedure as there is for antibiotics. we tested different plant extracts, their mixtures and phenolic acids on selected gram-positive (staphylococcus aureus, bacillus cereus, and listeria monocytogenes) and gram- ... | 2010 | 20171250 |
| potential of nitrogen gas (n2) to control psychrotrophs and mesophiles in raw milk. | pure n(2) gas was introduced in the headspace of test bottles containing raw milk that were then stored at either 6.0, 7.0, or 12.0 degrees c. treatment with n(2) significantly reduced the growth of total bacteria, and the growth of bacterial subgroups such as psychrotrophs, enterobacteria, protease- and lipase-producing bacteria, and listeria spp, and completely excluded bacillus cereus growth on myp plates. the inhibitory effect was maximal at 6.0 degrees c, and bacterial growth could be halte ... | 2010 | 19398315 |
| chemical composition and antimicrobial activity of essential oil of prangos ferulaceae (l.) lindl from iran. | the hydrodistilled essential oils of fruits and umbels of prangos ferulaceae (l.) lindl growing in iran were analysed by gas chromatograph-mass spectrometer. six compounds, comprising 93.7 and 94.7% of the total fruits and umbels oils, were identified, respectively. alpha-pinene and cis-ocimene are the major components of the oils. the essential oils show high antibacterial effect against bacillus cereus. | 2010 | 19452346 |
| optimization of fermentation conditions for production of anti-tmv extracellular ribonuclease by bacillus cereus using response surface methodology. | bacillus cereus zh14 was previously found to produce a new type of antiviral ribonuclease, which was secreted into medium and active against tobacco mosaic virus. in order to enhance the ribonuclease production, in this study the optimization of culture conditions using response surface methodology was done. the fermentation variables including culture temperature, initial ph, inoculum size, sucrose, yeast extract, mgso(4).7h(2)o, and kno(3) were considered for selection of significant ones by u ... | 2010 | 19466461 |
| the potential of flow cytometry in the study of bacillus cereus. | flow cytometry (fcm) is a rapid method allowing the acquisition of multiparametric data from thousands of individual cells within a sample. as well as measuring the intrinsic light scattering properties of cells, a plethora of fluorescent dyes may be employed to yield information on macromolecule content, surface antigens present or physiological status. despite fcm's indispensability within other fields e.g. immunology, it is underutilized within microbiological research. in this review, a stro ... | 2010 | 19486207 |
| burn wounds infected by contaminated water: case reports, review of the literature and recommendations for treatment. | first-aid education for the management of burns advocates cool running water over burnt skin to limit soft tissue damage. however, the water used may itself constitute a risk. we report three cases of severe invasive and necrotizing infection in patients who used or immersed themselves in contaminated water in an attempt to extinguish the fire following acute major burns. wound cultures from all patients yielded aeromonas hydrophila and two yielded bacillus cereus. one patient had a complex poly ... | 2010 | 19501977 |
| the catalytic property of 3-hydroxyisobutyrate dehydrogenase from bacillus cereus on 3-hydroxypropionate. | the mmsb gene product from bacillus cereus atcc14579 exhibits 3-hydroxypropionate dehydrogenase activity. it encodes the 32-kda enzyme protein composed of 292 amino acids. recombinant 3-hydroxyisobutyrate dehydrogenase (3-hibadh) was purified 100-fold from cell extract by ammonium sulfate fractionation and column chromatography. the enzyme catalyzed oxidation of 3-hydroxypropionate (3-hp) between ph 7.0 and 10.0 with optimal activity between 8.8 and 9.0. a km of 16.8 mm for 3-hp was calculated f ... | 2010 | 19517068 |
| screening of some kenyan medicinal plants for antibacterial activity. | eleven medicinal plants used by traditional healers in machakos and kitui district were screened, namely: ajuga remota benth, aloe secundiflora engl, amaranthus hybridus l, cassia didymobotrya fes, croton macrostachyus del, entada leptostachya harms, erythrina abyssinica dc, harrisonia abyssinica oliv, schkuhria pinnata o. ktze, terminalia kilimandscharica engl and ziziphus abyssinica hochst for potential antibacterial activity against four medically important bacterial strains, namely: bacillus ... | 2010 | 19548257 |
| an oxidant- and solvent-stable protease produced by bacillus cereus sv1: application in the deproteinization of shrimp wastes and as a laundry detergent additive. | the current increase in amount of shrimp wastes produced by the shrimp industry has led to the need in finding new methods for shrimp wastes disposal. in this study, an extracellular organic solvent- and oxidant-stable metalloprotease was produced by bacillus cereus sv1. maximum protease activity (5,900 u/ml) was obtained when the strain was grown in medium containing 40 g/l shrimp wastes powder as a sole carbon source. the optimum ph, optimum temperature, ph stability, and thermal stability of ... | 2010 | 19593670 |
| development of a pcr assay for identification of the bacillus cereus group species. | a pcr technique was developed as a reliable and rapid identification method for the bacillus cereus group species, based on a unique conserved sequence of the motb gene (encoding flagellar motor protein) from b. cereus, bacillus thuringiensis and bacillus anthracis. | 2010 | 19583794 |
| antibacterial compounds from mushrooms i: a lanostane-type triterpene and prenylphenol derivatives from jahnoporus hirtus and albatrellus flettii and their activities against bacillus cereus and enterococcus faecalis. | antibacterial bioassay-guided fractionation of two american mushroom species, jahnoporus hirtus and albatrellus flettii, led to the isolation and identification of their major antibacterial constituents: 3,11-dioxolanosta-8,24( z)-diene-26-oic acid (1) from j. hirtus and confluentin (2), grifolin (3), and neogrifolin (4) from a. flettii. compound 1 is a new lanostane-type triterpene. all purified compounds were evaluated for their ability to inhibit the growth of bacillus cereus and enterococcus ... | 2010 | 19644795 |
| survey of bacterial populations present in us-produced linerboard with high recycle content. | to survey paperboard products from 17 us mills for bacterial populations and for bacteria potentially harmful to human health. | 2010 | 19645765 |
| structure and applications of a rhamnolipid surfactant produced in soybean oil waste. | soybean oil soapstock was utilized as an alternative carbon source for the production of rhamnolipids by pseudomonas aeruginosa lbi strain. the chemical composition and properties of the rhamnolipid mixture obtained were determined to define its potential applications. the chemical characterization of the rhamnolipid has revealed the presence of ten different homologues. the monorhamnolipid rhac10c1) and the dirhamnolipid rha2c10c10 were the main components of the mixture that showed predominanc ... | 2010 | 19649781 |
| the preparation, germination properties and stability of superdormant spores of bacillus cereus. | to determine yields, germination and stability of superdormant bacillus cereus spores. | 2010 | 19674187 |
| growth and gaseous emissions of pure and mixed small intestinal bacterial cultures: effects of bile and vancomycin. | simultaneous cultivations in anaerobiosis, aerobiosis and with microaerobic gas mixture were used to clarify the bile (oxgall) effects on the pure and mixed cultures of enterobacterial strains in simulations in portable microbe enrichment unit (pmeu) linked with chempro100i((r)) gas detector. the effects of vancomycin were evaluated in aerobic cultures. growth and metabolic activity of cultures were also followed by measuring sugar consumption, ph alterations, and colony counts on bd chromagar o ... | 2010 | 19682874 |
| differential transfer dynamics of paw63 plasmid among members of the bacillus cereus group in food microcosms. | to assess the dynamics of plasmid transfer between bacillus thuringiensis and b. cereus in various food microcosms using the b. thuringiensis paw63 and staphylococcus aureus pub110 plasmids as models. | 2010 | 19709333 |
| a noninvasive near infrared system for detection of platelet components contaminated with bacteria. | platelet (plt) transfusion-associated bacterial sepsis has remained a substantial patient risk, primarily due to lacking effective and point-of-issue measures to detect bacterial contamination. this study describes near infrared (nir) spectroscopy to examine inoculated plts without sampling within a few seconds. | 2010 | 19694992 |
| treatment and outcome of traumatic endophthalmitis in open globe injury with retained intraocular foreign body. | our aim was to evaluate the incidence, risk factors, pathogens, use of systemic and intravitreal antibiotics, and outcome of traumatic endophthalmitis after open globe injury with retained intraocular foreign body (riofb). | 2010 | 19707031 |
| new approach for the detection of non-ribosomal peptide synthetase genes in bacillus strains by polymerase chain reaction. | bacillus strains produce non-ribosomal lipopeptides that can be grouped into three families: surfactins or lichenysins, iturins and fengycins or plispastatins. these biosurfactants show a broad spectrum of biological activities. to detect strains able to produce these lipopeptides, a new polymerase chain reaction screening approach was developed using degenerated primers based on the intraoperon alignment of adenylation and thiolation nucleic acid domains of all enzymes implicated in the biosynt ... | 2010 | 19730852 |
| examination of electron stains as a substitute for uranyl acetate for the ultrathin sections of bacterial cells. | electron staining reagents were examined to find a possible substitute for uranyl acetate (ua) in electron microscopy of bacterial ultrathin sections. four kinds of stains, platinum blue (pt-blue), oolong tea extract (ote), potassium permanganate (kmno(4)) and phosphotungstic acid (pta), were examined in comparison with ua either with or without post-staining with lead citrate (pb). electron microscopy was performed on sections from spurr-embedded cells of a gram-positive bacterium, bacillus cer ... | 2010 | 19767626 |
| evaluation of antimicrobial peptides as novel bactericidal agents for room temperature-stored platelets. | a single cost-effective pathogen inactivation approach would help to improve the safety of our nation's blood supply. several methods and technologies are currently being studied to help reduce bacterial contamination of blood components. there is clearly need for simple and easy-to-use pathogen inactivation techniques specific to plasma, platelets (plts), and red blood cells. | 2010 | 19761549 |
| antioxidant, anti-microbial and antimutagenicity activities of pistachio (pistachia vera) green hull extract. | antioxidant, anti-microbial and antimutagenicity activities of pistachio (ahmadaghaei variety) green hull extracts (crude and purified extracts) were studied. at first, different solvents were compared for determining of the best solvent for extraction of phenolic compounds from pistachio green hull. water and acetonitrile with 49.32 and 6.22 (mg of gallic acid equivalents/g sample) were the best and the worst solvent in the extraction of phenolic compounds, respectively. the antioxidant capacit ... | 2010 | 19781589 |
| acute encephalopathy of bacillus cereus mimicking reye syndrome. | we present an 11-year-old boy diagnosed as having acute encephalopathy and liver failure with the underlying condition of a metabolic dysfunction. he developed convulsions and severe consciousness disturbance following gastroenteritis after the ingestion of some fried rice. he showed excessive elevation of transaminases, non-ketotic hypoglycemia and hyperammonemia, which were presumed to reflect a metabolic dysfunction of the mitochondrial beta-oxidation, and he exhibited severe brain edema thro ... | 2010 | 19796886 |
| activity assay for nisin-like acidic bacteriocins using an optimal ph-conditioned gel matrix. | a new zymography for detecting nisin-like acidic bacteriocins was developed using a tricine-sodium dodecyl sulfate (sds) gel and an acidic gel matrix (ph 4.0). after electrophoresis, proteins in the tricine gel were electrotransferred to an optimal ph-conditioned gel matrix (op-cgm). the op-cgm was overlaid with indicator cells (bacillus cereus) embedded in nutrient broth soft agar (0.8%, w/v). antibacterial activity shown as a growth inhibition using b. cereus was detected at approximately 3.8k ... | 2010 | 19799850 |
| comparative analysis of the virulence of invertebrate and mammalian pathogenic bacteria in the oral insect infection model galleria mellonella. | infection of galleria mellonella by feeding a mixture of bacillus thuringiensis spores or vegetative bacteria in association with the toxin cry1c results in high levels of larval mortality. under these conditions the toxin or bacteria have minimal effects on the larva when inoculated separately. in order to evaluate whether g. mellonella can function as an oral infection model for human and entomo-bacterial pathogens, we tested strains of bacillus cereus, bacillus anthracis, enterococcus faecali ... | 2010 | 19800349 |
| in vitro effects on bacterial growth of phenoloxidase reaction products. | an active phenoloxidase preparation from the freshwater crayfish pacifastacus leniusculus exhibited a strong antibacterial effect in vitro on the bacteria aeromonas hydrophila, escherichia coli, streptococcus pneumoniae whereas a weaker but still significant effect against bacillus cereus, pseudomonas aeruginosa and staphylococcus aureus. in most cases reduction of bacterial growth was stronger when dopamine was used as substrate as compared to l-dopa. the effect on bacteria was abolished if no ... | 2010 | 19808037 |
| cluster of necrotizing enterocolitis in a neonatal intensive care unit: new mexico, 2007. | although the cause of necrotizing enterocolitis (nec) is unknown, infection control practices have been shown to play an important role in containing many outbreaks. we investigated the etiology of a cluster of nec in a level 3 neonatal intensive care unit and monitored for new cases following the implementation of enhanced infection control measures. | 2010 | 19822381 |
| the inha metalloproteases of bacillus cereus contribute concomitantly to virulence. | the virulence of bacillus cereus requires that bacteria have the capacity to colonize their host, degrade specific tissues, and circumvent the host immune system. to study this aspect of pathogenesis, we focused on three metalloproteases, inha1, inha2, and inha3, which share more than 66% identity. the expression of these metalloprotease genes was assessed by transcriptional fusions with a lacz reporter gene. the expression profiles suggest a complementary time course of inha production. indeed, ... | 2010 | 19837797 |
| characterization of corrosive bacterial consortia isolated from petroleum-product-transporting pipelines. | microbiologically influenced corrosion is a problem commonly encountered in facilities in the oil and gas industries. the present study describes bacterial enumeration and identification in diesel and naphtha pipelines located in the northwest and southwest region in india, using traditional cultivation technique and 16s rdna gene sequencing. phylogenetic analysis of 16s rrna sequences of the isolates was carried out, and the samples obtained from the diesel and naphtha-transporting pipelines sh ... | 2010 | 19844704 |