Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| antimicrobial activity of alexidine alone and associated with n-acetylcysteine against enterococcus faecalis biofilm. | the purpose of this study was to assess the efficacy of alexidine (alx), alone and combined with n-acetylcysteine (nac), in eradicating two enterococcus faecalis strain biofilms. the biofilms of e. faecalis atcc 29212 and the clinical isolate e. faecalis d1 were grown in the mbec-high-throughput device for 24 h and were exposed to five twofold dilutions of alx (2%-0.007 8%) alone and combined with 100 mg⋅ml(-1) nac, for 1 and 5 min. eradication was defined as 100% kill of biofilm bacteria. the s ... | 2013 | 23970139 |
| mortality in kittens is associated with a shift in ileum mucosa-associated enterococci from enterococcus hirae to biofilm-forming enterococcus faecalis and adherent escherichia coli. | approximately 15% of foster kittens die before 8 weeks of age, with most of these kittens demonstrating clinical signs or postmortem evidence of enteritis. while a specific cause of enteritis is not determined in most cases, these kittens are often empirically administered probiotics that contain enterococci. the enterococci are members of the commensal intestinal microbiota but also can function as opportunistic pathogens. given the complicated role of enterococci in health and disease, it woul ... | 2013 | 23966487 |
| lipoteichoic acid of enterococcus faecalis induces the expression of chemokines via tlr2 and pafr signaling pathways. | enterococcus faecalis is one of the most common opportunistic pathogens responsible for nosocomial infections, and its lta is known as an important virulence factor causing inflammatory responses. as chemokines play a key role in inflammatory diseases by triggering leukocyte infiltration into the infection site, we purified eflta and investigated its effect on the expression of chemokines, ip-10, mip-1α, and mcp-1, in murine macrophages. eflta induced the expression of these chemokines at the mr ... | 2013 | 23964117 |
| influence of various herbal irrigants as a final rinse on the adherence of enterococcus faecalis by fluorescence confocal laser scanning microscope. | the aim of this study was to assess the antibacterial efficacy of three different herbal irrigants against enterococcus faecalis. | 2013 | 23956540 |
| quantitative evaluation of apical extrusion of intracanal bacteria using k3, mtwo, race and protaper rotary systems: an in vitro study. | this aim of this study was to evaluate the number of bacteria extruded apically during root canal preparation using four rotary instrumentation techniques. | 2013 | 23956529 |
| cold-shock rna-binding protein cspr is also exposed to the surface of enterococcus faecalis. | cspr has been characterized recently as a cold-shock rna-binding protein in enterococcus faecalis, a natural member of the gastro-intestinal tract capable of switching from a commensal relationship with the host to an important nosocomial pathogen. in addition to its involvement in the cold-shock response, cspr also plays a role in the long-term survival and virulence of e. faecalis. in the present study, we demonstrated that anti-cspr immune rabbit serum protected larvae of galleria mellonella ... | 2013 | 23955430 |
| the crystal structure of d-mandelate dehydrogenase reveals its distinct substrate and coenzyme recognition mechanisms from those of 2-ketopantoate reductase. | d-mandelate dehydrogenases (d-mandhs), belonging to a new d-2-hydroxyacid dehydrogenase family, catalyze the conversion between benzoylformate and d-mandelate using nad as a coenzyme. we determined the first d-mandh structure, that of mandh2 from enterococcus faecalis iam10071. the overall structure showed mandh2 has a similar fold to 2-ketopantoate reductase (kpr), which catalyzes the conversion of 2-ketopantoate to d-pantoate using nadp as a coenzyme. they share conserved catalytic residues, i ... | 2013 | 23954635 |
| comparative antibacterial efficacies of hydrodynamic and ultrasonic irrigation systems in vitro. | to ensure root canal treatment success, endodontic microbiota should be efficiently reduced. the in vitro bactericidal effects of a hydrodynamic system and a passive ultrasonic irrigation system were compared. | 2013 | 23953293 |
| organism-adapted specificity of the allosteric regulation of pyruvate kinase in lactic acid bacteria. | pyruvate kinase (pyk) is a critical allosterically regulated enzyme that links glycolysis, the primary energy metabolism, to cellular metabolism. lactic acid bacteria rely almost exclusively on glycolysis for their energy production under anaerobic conditions, which reinforces the key role of pyk in their metabolism. these organisms are closely related, but have adapted to a huge variety of native environments. they include food-fermenting organisms, important symbionts in the human gut, and ant ... | 2013 | 23946717 |
| enterococcus faecalis endogenous endophthalmitis from valvular endocarditis. | we report a case of a 74-year-old female, with a mitral heart valve, who presented with pain and blurred vision in the right eye for 2 days. her visual acuity was light perception (lp) in the right eye and 20/40 in the left eye. slit lamp examination showed corneal edema and hypopyon, and a view of the right fundus was impossible. echography showed vitreous condensation. one day after presentation, the patient developed acute lung edema requiring hospitalization, so she was not submitted to vitr ... | 2013 | 23936701 |
| [peripheral venous catheter-related e. faecalis sepsis with fatal outcome]. | a 82 year-old man was admitted with a facial herpes zoster reactivation and a zoster-related meningoencephalitis. he was treated with aciclovir over a peripheral venous catheter that was placed in the right elbow pit. the meningoencephalitis subsided without neurological deficits, but the patient developed a catheter-associated phlebitis. an oral cephalosporin was prescribed and the patient discharged. after developing a fever and general discomfort he was readmitted to hospital three days later ... | 2013 | 23934591 |
| distribution of antimicrobial-resistant lactic acid bacteria in natural cheese in japan. | to determine and compare the extent of contamination caused by antimicrobial-resistant lactic acid bacteria (lab) in imported and domestic natural cheeses on the japanese market, lab were isolated using deman, rogosa and sharpe (mrs) agar and mrs agar supplemented with six antimicrobials. from 38 imported and 24 japanese cheeses, 409 lab isolates were obtained and their antimicrobial resistance was tested. the percentage of lab resistant to dihydrostreptomycin, erythromycin, and/or oxytetracycli ... | 2013 | 23930694 |
| rv3168 phosphotransferase activity mediates kanamycin resistance in mycobacterium tuberculosis. | tuberculosis is a worldwide epidemic disease caused by mycobacterium tuberculosis, with an estimated one-third of the human population currently affected. treatment of this disease with aminoglycoside antibiotics has become less effective owing to antibiotic resistance. recent determination of the crystal structure of the m. tuberculosis rv3168 protein suggests a structure similar to that of enterococcus faecalis aph(3')-iiia, and that this protein may be an aminoglycoside phosphotransferase. to ... | 2013 | 23928847 |
| antibacterial activity of the recombinant antimicrobial peptide ib-amp4 from impatiens balsamina and its synergy with other antimicrobial agents against drug resistant bacteria. | ib-amp4 is an antimicrobial peptide of impatiens balsamina (balsaminaceae). ib-amp4 was produced as a recombinant peptide and in this study its antimicrobial activity against human bacterial pathogens was investigated. ib-amp4 was bactericidal against both gram positive and gram negative bacteria with mic values between 0.49 and 3.5 microm in sensitive species. a genuine synergistic effect was achieved when ib-amp4 was employed in combination with the plant monoterpene thymol against drug-resist ... | 2013 | 23923648 |
| zebrafish as a novel vertebrate model to dissect enterococcal pathogenesis. | enterococcus faecalis is an opportunistic pathogen responsible for a wide range of life-threatening nosocomial infections, such as septicemia, peritonitis, and endocarditis. e. faecalis infections are associated with a high mortality and substantial health care costs and cause therapeutic problems due to the intrinsic resistance of this bacterium to antibiotics. several factors contributing to e. faecalis virulence have been identified. due to the variety of infections caused by this organism, n ... | 2013 | 24002065 |
| functional diversity of tandem substrate-binding domains in abc transporters from pathogenic bacteria. | the atp-binding cassette (abc) transporter glnpq is an essential uptake system for amino acids in gram-positive pathogens and related nonpathogenic bacteria. the transporter has tandem substrate-binding domains (sbds) fused to each transmembrane domain, giving rise to four sbds per functional transporter complex. we have determined the crystal structures and ligand-binding properties of the sbds of glnpq from enterococcus faecalis, streptococcus pneumoniae, and lactococcus lactis. the tandem sbd ... | 2013 | 23994008 |
| [evaluation on antimicrobial efficacy of fufang bingpeng irrigant: an in vitro study]. | to evaluate the antimicrobial efficacy of fufang bingpeng irrigant and bingpeng irrigant on four endodontic pathogen bacteria. | 2013 | 23991570 |
| calpastatin prevents nf-κb-mediated hyperactivation of macrophages and attenuates colitis. | calpain enzymes proteolytically modulate cellular function and have been implicated in inflammatory diseases. in this study, we found that calpain levels did not differ between intestinal tissues from inflammatory bowel disease (ibd) patients and healthy controls, but ibd tissues showed increased levels of the endogenous calpain inhibitor, calpastatin (cast). to investigate the role of cast in the immune system during ibd, mice were x-ray irradiated, reconstituted with either cast-knockout (ko) ... | 2013 | 23986533 |
| antibiotic-resistance and virulence genes in enterococcus isolated from tropical recreational waters. | the prevalence of enterococci harboring tetracycline- and vancomycin-resistance genes, as well as the enterococcal surface protein (esp) has mostly been determined in clinical settings, but their prevalence in tropical recreational waters remains largely unknown. the present study determined the prevalence of tetm (tetracycline-resistance), vana and vanb (vancomycin-resistance) in the bacterial and viral fractions, enterococci and their induced phages isolated from tropical recreational marine a ... | 2013 | 23981868 |
| biological evaluation of hyperforin and its hydrogenated analogue on bacterial growth and biofilm production. | bacterial biofilms are organized communities of microorganisms, embedded in a self-produced matrix, growing on a biotic surface and resistant to many antimicrobial agents when associated with a medical device. these biofilms require the development of new strategies for the prevention and treatment of infectious disease, including the potential use of natural products. one interesting natural product example is hypericum, a plant genus that contains species known to have antimicrobial properties ... | 2013 | 23981190 |
| additive effect of enterococcus faecium on enterococcal bloodstream infections: a 14-year study in a swiss tertiary hospital. | we investigated whether an increase in enterococcal bloodstream infections (bsis) depends on the emergence of enterococcus faecium in an area with low vancomycin-resistant enterococci prevalence. from 1999 to 2012, a linear increase in e. faecium bsi rates (0.009 per 1,000 patient-days per year; p < .001) was noted. enterococcus faecalis bsi rates remained stable. | 2013 | 24018931 |
| risk factors for and epidemiology of community-onset vancomycin-resistant enterococcus faecalis in southeast michigan. | given the known link between vancomycin-resistant enterococcus faecalis (vref) and vancomycin-resistant staphylococcus aureus (vrsa), the recent increase in prevalence of vref in southeast michigan has raised concerns about the presence of a large "community" reservoir of vref. efforts to control its spread face some important challenges. | 2013 | 24011554 |
| culture independent raman spectroscopic identification of urinary tract infection pathogens: a proof of principle study. | urinary tract infection (uti) is a very common infection. up to every second woman will experience at least one uti episode during her lifetime. the gold standard for identifying the infectious microorganisms is the urine culture. however, culture methods are time-consuming and need at least 24 h until the results are available. here, we report about a culture independent identification procedure by using raman microspectroscopy in combination with innovative chemometrics. we investigated, for t ... | 2013 | 24010860 |
| correlations between income inequality and antimicrobial resistance. | the aim of this study is to investigate if correlations exist between income inequality and antimicrobial resistance. this study's hypothesis is that income inequality at the national level is positively correlated with antimicrobial resistance within developed countries. | 2013 | 24009734 |
| comparative analysis of tunisian wild crataegus azarolus (yellow azarole) and crataegus monogyna (red azarole) leaf, fruit, and traditionally derived syrup: phenolic profiles and antioxidant and antimicrobial activities of the aqueous-acetone extracts. | quantitative and qualitative analyses of the yellow and red azarole phenolic extracts prepared from leaf, fruit peel/pulp, and syrup were comparatively investigated. the yellow azarole was found significantly richer in polyphenols than the red-fruit species. hyperoside was the main phenolic in both yellow and red azarole leaves and only in yellow fruits, whereas procyanidin b2 was the major compound in red fruits. yellow azarole leaf and fruit peel extracts exhibited the strongest antioxidant ac ... | 2013 | 24070066 |
| an encapsulated drug delivery system for recalcitrant urinary tract infection. | one of the hallmarks of urinary tract infection, a serious global disease, is its tendency to recur. uropathogenic bacteria can invade cells lining the bladder, where they form longer-term intracellular reservoirs shielded from antibiotics, re-emerging at a later date to initiate flare-ups. in these cases, only lengthy systemic antibiotic treatment can eradicate all the reservoirs. yet, long courses of antibiotics are not ideal, as they can lead to side effects and an increase in antibiotic resi ... | 2013 | 24068180 |
| technological characterisation, antibiotic susceptibility and antimicrobial activity of wild-type leuconostoc strains isolated from north italian traditional cheeses. | genotypic and technological properties, antibiotic susceptibility and antimicrobial activity of 35 leuconostoc strains, isolated from different italian raw milk cheeses, were investigated. rapd-pcr was used to study genetic variability and to distinguish closely related strains. the results showed a high degree of heterogeneity among isolates. all the strains had weak acidifying activity and showed low proteolytic and lipolytic activities. reduction activity, was generally low. all the leuconost ... | 2013 | 24067095 |
| bioactive nanofibrous scaffolds for regenerative endodontics. | here we report the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel antibiotic-containing scaffolds. metronidazole (met) or ciprofloxacin/(cip) was mixed with a polydioxanone (pds)polymer solution at 5 and 25 wt% and processed into fibers. pds fibers served as a control. scanning electron microscopy (sem), fourier-transform infrared spectroscopy (ftir), tensile testing, and high-performance liquid chromatography (hplc) were used to assess fiber morpho ... | 2013 | 24056225 |
| enterococcal sex pheromones: signaling, social behavior, and evolution. | in enterococcus faecalis, lateral transfer of conjugative plasmids that encode antibiotic resistance and virulence determinants can be induced by peptide sex pheromones. the tetracycline-resistance plasmid pcf10 represents a paradigm for illustrating important conserved features of a large family of pheromone-responsive enterococcal plasmids. the pheromone is released into the growth medium by plasmid-free recipient cells and sensed by plasmid-containing donors. the activity of the pheromone is ... | 2013 | 24050179 |
| life-threatening bacteremia and pyonephrosis caused by enterococcus hirae. | 2013 | 24035432 | |
| interference in pheromone-responsive conjugation of a high-level bacitracin resistant enterococcus faecalis plasmid of poultry origin. | the current study reports on contact interference of a high-level bacitracin- resistant pheromone-responsive plasmid of enterococcus faecalis strain 543 of poultry origin during conjugative transfer of bcr antimicrobial resistance genes using a polyclonal antiserum aggregation substance(44-560) (as). after induction with pheromones produced by the recipient strain e. faecalis jh2-2, clumping of the donor e. faecalis strain 543 was observed as well as high transfer frequencies of bcr in short tim ... | 2013 | 24030654 |
| in vitro efficacy of fosfomycin-based combinations against clinical vancomycin-resistant enterococcus isolates. | vancomycin-resistant (vr) enterococci (vre) are increasingly important nosocomial pathogens, commonly causing catheter-related urinary tract infections or vascular catheter-related bloodstream infections. in this study, 10 enterococcus faecium and 9 enterococcus faecalis different pulsed-field gel electrophoresis genome-type vr clinical isolates were detected. the potential role of fosfomycin-based combination regimens for biofilm-related vre infection is in vitro evaluated. anti-vre activities ... | 2013 | 24029433 |
| evaluation of the toxicity and antimicrobial activity of hydroethanolic extract of arrabidaea chica (humb. & bonpl.) b. verl. | arrabidaea chica (bignoniaceae) is a vine native to the amazon rainforest, popularly known as "crajiru" and whose infusion and decoction of the leaves are used to treat diseases such as gastric ulcers, inflammations, infections, anemia, herpes, jaundice among others. it is also used as a natural dye. this work aimed to evaluate the in vitro and in vivo toxicity, antimicrobial activity including analysis of chemical constitution of the hydroethanolic extract of the leaves of arrabidaea chica (hea ... | 2013 | 24070833 |
| survival, distribution, and translocation of enterococcus faecalis and implications for pregnant mice. | pregnant mothers are susceptible to bacterial infections, which may compromise the health of mothers and offspring. enterococcus faecalis is a ubiquitous species found in food, restaurants, and hospitals where pregnant woman frequently become exposed to this bacterium. however, the survival, distribution, translocation, and corresponding influence of e. faecalis have not been investigated during the pregnancy period, when the mother and fetus are susceptible to bacterial infection. in this study ... | 2013 | 24131236 |
| vancomycin-resistant enterococci. | vancomycin-resistant enterococci (vre) consist mainly of enterococcus faecalis and e faecium, the latter mostly hospital-acquired. in addition, e gallinarum and e casseliflavus are intrinsically vancomycin-resistant and are community-acquired. vre have become common in many hospitals throughout the world and, once established, are very difficult to eradicate. vre are difficult to treat; therefore, infection control measures in hospitals are of prime importance in preventing the establishment of ... | 2013 | 24094380 |
| a current perspective on daptomycin for the clinical microbiologist. | daptomycin is a lipopeptide antimicrobial with in vitro bactericidal activity against gram-positive bacteria that was first approved for clinical use in 2004 in the united states. since this time, significant data have emerged regarding the use of daptomycin for the treatment of serious infections, such as bacteremia and endocarditis, caused by gram-positive pathogens. however, there are also increasing reports of daptomycin nonsusceptibility, in staphylococcus aureus and, in particular, enteroc ... | 2013 | 24092854 |
| enhanced expression of fucosyl ga1 in the digestive tract of immune-deficient scid, nude and pigr(-/-) mice. | fucosylation of ga1 in murine intestinal epithelia occurs through transcriptional induction of α1,2-fucosyltransferase along with bacterial infection, but the mechanism has not been clearly characterized as to whether it is induced as a result of an immune response to bacteria or of genetic manipulation of the host by bacteria. accordingly, we analysed the expression of fucosyl ga1 (fga1) and fucosyltransferase activity in the digestive tracts of immune-deficient scid, nude and pigr(-/-) mice. i ... | 2013 | 24089533 |
| the two functional enoyl-acyl carrier protein reductases of enterococcus faecalis do not mediate triclosan resistance. | enoyl-acyl carrier protein (enoyl-acp) reductase catalyzes the last step of the elongation cycle in the synthesis of bacterial fatty acids. the enterococcus faecalis genome contains two genes annotated as enoyl-acp reductases, a fabi-type enoyl-acp reductase and a fabk-type enoyl-acp reductase. we report that expression of either of the two proteins restores growth of an escherichia coli fabi temperature-sensitive mutant strain under nonpermissive conditions. in vitro assays demonstrated that bo ... | 2013 | 24085780 |
| antibacterial efficacy of mangifera indica l. kernel and ocimum sanctum l. leaves against enterococcus faecalis dentinal biofilm. | the enterococcus faecalis biofilm in the root canal makes it difficult to be eradicated by the conventional irrigants with no toxicity to the tissues. hence, plant products with least side effects are explored for their use as irrigants in the root canal therapy. | 2013 | 24082577 |
| protection of enterococcus faecalis in mixed cultures with carbapenemase-producing escherichia coli and bacteroide fragilis: effect of the bacterial load. | this study explores effects of ph and inoculum size on imipenem versus tigecycline activity against e. coli, b. fragilis and e. faecalis, both in individual and mixed cultures. | 2013 | 24080888 |
| ireb, a ser/thr kinase substrate, influences antimicrobial resistance in enterococcus faecalis. | enterococcus faecalis is a gram-positive bacterium that is a major cause of hospital-acquired infections, in part due to its intrinsic resistance to cephalosporins. the mechanism that confers intrinsic cephalosporin resistance in enterococci remains incompletely defined. previously, we have shown that the ser/thr protein kinase and phosphatase pair irek and irep act antagonistically to regulate cephalosporin resistance in e. faecalis. we hypothesize that irek senses antibiotic-induced cell wall ... | 2013 | 24080657 |
| high-dose daptomycin therapy for left-sided infective endocarditis: a prospective study from the international collaboration on endocarditis. | the use of daptomycin in gram-positive left-sided infective endocarditis (ie) has significantly increased. the purpose of this study was to assess the influence of high-dose daptomycin on the outcome of left-sided ie due to gram-positive pathogens. this was a prospective cohort study based on 1,112 cases from the international collaboration on endocarditis (ice)-plus database and the ice-daptomycin substudy database from 2008 to 2010. among patients with left-sided ie due to staphylococcus aureu ... | 2013 | 24080644 |
| icesluvan, a 94-kilobase mosaic integrative conjugative element conferring interspecies transfer of vanb-type glycopeptide resistance, a novel bacitracin resistance locus, and a toxin-antitoxin stabilization system. | a 94-kb integrative conjugative element (icesluvan) transferable to enterococcus faecium and enterococcus faecalis from an animal isolate of streptococcus lutetiensis consists of a mosaic of genetic fragments from different gram-positive bacteria. a variant of icesluvan was confirmed in s. lutetiensis from a patient. a complete tn5382/tn1549 with a vanb2 operon is integrated into a streptococcal icesde3396-like region harboring a putative bacteriophage exclusion system, a putative agglutinin rec ... | 2013 | 24078615 |
| microwave-assisted synthesis, characterization and antibacterial activity of ag/zno nanoparticles supported bentonite clay. | composites of silver-zinc oxide nanoparticles supported on bentonite clay were synthesized by the microwave-assisted synthesis method for use as an antibacterial material. silver nitrate was used as the precursor of silver nanoparticles while zinc oxide nanoparticles were commercially sourced. the composites were characterized by powder x-ray diffraction (xrd), transmission electron microscope (tem), fourier transform infrared (ftir) and bet surface area measurements. xrd spectra showed peaks of ... | 2013 | 24076479 |
| production of microbial medium from defatted brebra (milletia ferruginea) seed flour to substitute commercial peptone agar. | to investigate and optimize microbial media that substitute peptone agar using brebra seed defatted flour. | 2013 | 24075344 |
| enterococcus faecalis infective endocarditis: focus on clinical aspects. | enterococcus faecalis infective endocarditis (ie) is a disease of increasing importance, with more patients infected, increasing frequency of health-care associated infections and increasing incidence of antimicrobial resistances. the typical clinical presentation is a subacute course with fever, malaise and generalized aches, difficult to distinguish from other more common diseases. of paramount importance is transthoracic- and transesophageal-echocardiography to establish the diagnosis. at the ... | 2013 | 24073680 |
| first isolation of oleate-dependent enterococcus faecalis small-colony variants from the umbilical exudate of a paediatric patient with omphalitis. | an oleate-dependent enterococcus faecalis isolate representing small-colony variants (scvs) was isolated from the umbilical exudate of a 31-month-old japanese male patient in nagano children's hospital, azumino, japan. the patient had been suffering from recurrent omphalitis since early infancy. the initial e. faecalis scv isolate formed small colonies on sheep blood agar plates and tiny colonies on chocolate and modified drigalski agar, although no visible growth was observed in hk-semi solid m ... | 2013 | 24072765 |
| survival of enterococcus faecalis during alkaline stress: changes in morphology, ultrastructure, physiochemical properties of the cell wall and specific gene transcripts. | the aim of this investigation was to study the biochemical mechanisms employed by the endodontic pathogen enterococcus faecalis to survive alkaline environment during biofilm formation. | 2013 | 24112733 |
| biocide and copper tolerance in enterococci from different sources. | antimicrobial resistance in enterococci is a matter of concern. a collection of 272 strains (including 107 enterococcus faecalis and 165 enterococcus faecium strains) isolated from meat and dairy products, seafood, vegetable foods, wildflowers, animal feces (ewe, goat, horse, mule), and hospitals were tested for sensitivity to biocides of different classes (quaternary ammonium compounds, a bisphenol, and a biguanide) and copper sulfate. most isolates were inhibited at 25 mg of benzalkonium chlor ... | 2013 | 24112585 |
| combined dielectrophoresis-raman setup for the classification of pathogens recovered from the urinary tract. | rapid and effective methods of pathogen identifications are of major interest in clinical microbiological analysis to administer timely tailored antibiotic therapy. raman spectroscopy as a label-free, culture-independent optical method is suitable to identify even single bacteria. however, the low bacteria concentration in body fluids makes it difficult to detect their characteristic molecular fingerprint directly in suspension. therefore, in this study, raman spectroscopy is combined with diele ... | 2013 | 24125497 |
| a zn-dependent metallopeptidase is responsible for sensitivity to lsbb, a class ii leaderless bacteriocin of lactococcus lactis subsp. lactis bgmn1-5. | lactococcus lactis subsp. lactis bgmn1-5 produces a leaderless class ii bacteriocin called lsbb. to identify the receptor for lsbb, a cosmid library of the lsbb-sensitive strain bgmn1-596 was constructed. about 150 cosmid clones were individually isolated and transferred to lsbb-resistant mutants of bgmn1-596. cosmid pazilcos/mn2, carrying a 40-kb insert, was found to restore lsbb sensitivity in lsbb-resistant mutants. further subcloning revealed that a 1.9-kb fragment, containing only one open ... | 2013 | 24123824 |
| pestalotiopens a and b: stereochemically challenging flexible sesquiterpene-cyclopaldic acid hybrids from pestalotiopsis sp. | from the endophytic fungus pestalotiopsis sp. isolated from the leaves of the chinese mangrove, rhizophora mucronata, two novel hybrid sesquiterpene-cyclopaldic acid metabolites with an unusual carbon skeleton, named pestalotiopens a and b, were obtained, together with the already known phytotoxin altiloxin b. pestalotiopen b even contains a third, triketide-derived module. the constitutions and the absolute configurations of the new metabolites and of altiloxin b were unambiguously determined b ... | 2013 | 24123472 |
| effect of modified nonequilibrium plasma with chlorhexidine digluconate against endodontic biofilms in vitro. | nonequilibrium plasma has been reported to effectively kill enterococcus faecalis in endodontic biofilm compared with chlorhexidine digluconate (chx). the purpose of this study was to evaluate the antimicrobial in vitro activity of modified nonequilibrium plasma with chx against e. faecalis and multispecies biofilms on bovine dentin discs. | 2013 | 24139270 |
| antimicrobial substantivity of alexidine and chlorhexidine in dentin. | the use of irrigating solutions with long-lived activity helps to avoid failure of endodontic therapy. the aim of the present study was to evaluate and compare the antimicrobial substantivity against enterococcus faecalis by using as carrier a dentin-volumetric unit exposed to chlorhexidine (chx) and alexidine (alx). | 2013 | 24139264 |
| effect of smear layer against disinfection protocols on enterococcus faecalis-infected dentin. | this study examined the effect of the smear layer on the antibacterial effect of different disinfecting solutions in infected dentinal tubules. | 2013 | 24139261 |
| effectiveness of antibiotic medicaments against biofilm formation of enterococcus faecalis and porphyromonas gingivalis. | in this study we compared the antibacterial effect of triple antibiotic paste (tap), double antibiotic paste (dap), and calcium hydroxide [ca(oh)2] against enterococcus faecalis and porphyromonas gingivalis biofilm. | 2013 | 24139259 |
| in vivo quantitative evaluation of live and dead bacteria in root canal infection by using propidium monoazide with real-time pcr. | for selective detection of viable bacteria with molecular methods, propidium monoazide (pma) treatment has been successfully applied to a wide range of bacteria. the purpose of this study was to compare the quantity of live cells with the total amounts of both live and dead cells before and after chemomechanical preparation by using pma in combination with real-time polymerase chain reaction (qpcr). | 2013 | 24139254 |
| ammonia sanitisation of sewage sludge using urea. | the aim of the study was to develop a simple, low-cost treatment for sewage sludge using urea as a sanitising agent. sewage sludge was spiked with enterococcus faecalis and salmonella typhimurium, treated with 0.5, 1, 1.5 and 2% w/w urea at laboratory scale, and the viability was monitored during 4 months of storage at 4, 10 and 22 °c (only 0.5%). a linear relationship was identified between salmonella spp. inactivation rate and ammonia (nh3) concentration. temperature had a positive impact on s ... | 2013 | 24185072 |
| in vitro evaluation of the minimum bactericidal concentrations of different root-end filling materials. | the aim of this study was to evaluate the minimum bactericidal concentrations (mbc) of root-end filling materials proroot mta, mta angelus and irm. | 2013 | 24171975 |
| calcium enriched mixture and mineral trioxide aggregate activities against enterococcus faecalis in presence of dentin. | the purpose of this in vitro study was to compare the antibacterial activity of calcium enriched mixture (cem) with proroot mineral trioxide aggregate (mta) against enterococcus faecalis (e. faecalis) in the presence/absence of dentin powder. | 2013 | 24171028 |
| does cmv infection impact the virulence of enterococcus faecalis? | 2013 | 24162854 | |
| prevalence and antimicrobial resistance of canine urinary tract pathogens. | this study aims to describe the incidence and risk factors for positive urinary tract culture, the prevalence of urinary tract pathogens in single organism and mixed cultures and changes in their antimicrobial resistance over 10 years. a retrospective review of computer records detailing canine urine samples submitted between august 1999 and september 2009 for culture and sensitivity in a uk tertiary referral hospital is described. 17.5 per cent of 5923 samples (670 of 4530 dogs) were positive c ... | 2013 | 24158327 |
| expression of the agmatine deiminase pathway in enterococcus faecalis is activated by the agur regulator and repressed by ccpa and pts(man) systems. | although the agmatine deiminase system (agdi) has been investigated in enterococcus faecalis, little information is available with respect to its gene regulation. in this study we demonstrate that the presence of exogenous agmatine induces the expression of agu genes in this bacterium. in contrast to the homologous and extensively characterized agdi system of s. mutants, the agubdac operon in e. faecalis is not induced in response to low ph. in spite of this, agmatine catabolism in this bacteriu ... | 2013 | 24155893 |
| evaluation of the antibacterial efficacy of omeprazole with sodium hypochlorite as an endodontic irrigating solution- an invivo study. | proton pump inhibitors are used in endodontic disinfection of root canals for elimination of enterococcus faecalis. this invivo study on wistner rats is carried out to determine antimicrobial efficacy of proton pump inhibitor in combination with sodium hypochlorite & mixture of isomer of tetracycline, acid and detergent (mtad) against e. faecalis materials & methods: periapical lesions were induced on the 30 incisor teeth of 30 male wistner rats (10per group). after 28 days, root canals of each ... | 2013 | 24155586 |
| adhesion and virulence factor properties of enterococci isolated from clinical samples in iran. | enterococci rank among leading causes of nosocomial bacteremia, urinary tract infections and community acquired endocarditis. the aim of the present study was to investigate the presence of virulence factors in enterococci strains isolated from clinical samples in iranian educational hospitals. | 2013 | 24152500 |
| colon macrophages polarized by commensal bacteria cause colitis and cancer through the bystander effect. | intestinal commensal bacteria have recently been shown to trigger macrophages to produce diffusible clastogens (or chromosome-breaking factors) through a bystander effect (bse) that mediates dna damage and induces chromosomal instability in neighboring cells. colon macrophages appear central to colon carcinogenesis and bse through the expression of tumor necrosis factor-α (tnf-α) and cyclooxygenase-2 (cox-2). the former induces netrin-1, a regulator of intestinal epithelial cell apoptosis, and t ... | 2013 | 24151540 |
| photocatalytic antibacterial effects are maintained on resin-based tio2 nanocomposites after cessation of uv irradiation. | photocatalysis induced by tio2 and uv light constitutes a decontamination and antibacterial strategy utilized in many applications including self-cleaning environmental surfaces, water and air treatment. the present work reveals that antibacterial effects induced by photocatalysis can be maintained even after the cessation of uv irradiation. we show that resin-based composites containing 20% tio2 nanoparticles continue to provide a pronounced antibacterial effect against the pathogens escherichi ... | 2013 | 24146793 |
| the daf-16/foxo transcription factor functions as a regulator of epidermal innate immunity. | the caenorhabditis elegans daf-16 transcription factor is critical for diverse biological processes, particularly longevity and stress resistance. disruption of the daf-2 signaling cascade promotes daf-16 activation, and confers resistance to killing by pathogenic bacteria, such as pseudomonas aeruginosa, staphylococcus aureus, and enterococcus faecalis. however, daf-16 mutants exhibit similar sensitivity to these bacteria as wild-type animals, suggesting that daf-16 is not normally activated by ... | 2013 | 24146615 |
| focal targeting by human β-defensin 2 disrupts localized virulence factor assembly sites in enterococcus faecalis. | virulence factor secretion and assembly occurs at spatially restricted foci in some gram-positive bacteria. given the essentiality of the general secretion pathway in bacteria and the contribution of virulence factors to disease progression, the foci that coordinate these processes are attractive antimicrobial targets. in this study, we show in enterococcus faecalis that seca and sortase a, required for the attachment of virulence factors to the cell wall, localize to discrete domains near the s ... | 2013 | 24191013 |
| measuring the effects of bacteria on c. elegans behavior using an egg retention assay. | c. elegans egg-laying behavior is affected by environmental cues such as osmolarity and vibration. in the total absence of food c. elegans also cease egg-laying and retain fertilized eggs in their uterus. however, the effect of different sources of food, especially pathogenic bacteria and particularly enterococcus faecalis, on egg-laying behavior is not well characterized. the egg-in-worm (eiw) assay is a useful tool to quantify the effects of different types of bacteria, in this case e. faecali ... | 2013 | 24192811 |
| bactericidal efficacy of photodynamic therapy against enterococcus faecalis in infected root canals: a systematic literature review. | the aim was to review the bactericidal efficacy of photodynamic therapy (pdt) against enterococcus faecalis (e. faecalis) in infected root canals. | 2013 | 24192536 |
| epidemic escherichia coli st131 and enterococcus faecium st17 in coastal marine sediments from an italian beach. | fecal indicator bacteria (fib) are used worldwide to assess water quality in coastal environments, but little is known about their genetic diversity and pathogenicity. this study examines the prevalence, antimicrobial resistance, virulence, and genetic diversity of fib isolated from marine sediments from a central adriatic seaside resort. fib, recovered from 6 out of 7 sites, were significantly more abundant at sampling stations 300 m offshore than close to the shore. escherichia coli accounted ... | 2013 | 24195439 |
| [necrotizing pneumonia due to enterococcus faecalis in an immunocompromised patient of 67 years]. | 2013 | 24211001 | |
| differences in visceral fat and fat bacterial colonization between ulcerative colitis and crohn's disease. an in vivo and in vitro study. | crohn's disease (cd) is notably characterized by the expansion of visceral fat with small adipocytes expressing a high proportion of anti-inflammatory genes. conversely, visceral fat depots in ulcerative colitis (uc) patients have never been characterized. our study aims were a) to compare adipocyte morphology and gene expression profile and bacterial translocation in omental (om) and mesenteric (mes) adipose tissue of patients with uc and cd, and b) to investigate the effect of bacterial infect ... | 2013 | 24205244 |
| rapid kill-novel endodontic sealer and enterococcus faecalis. | with growing concern over bacterial resistance, the identification of new antimicrobial means is paramount. in the oral cavity microorganisms are essential to the development of periradicular diseases and are the major causative factors associated with endodontic treatment failure. as quaternary ammonium compounds have the ability to kill a wide array of bacteria through electrostatic interactions with multiple anionic targets on the bacterial surface, it is likely that they can overcome bacteri ... | 2013 | 24223159 |
| reply to "breakthrough bacteremia by linezolid-susceptible enterococcus faecalis under linezolid treatment in a severe polytrauma patient". | 2013 | 24222609 | |
| breakthrough bacteremia by linezolid-susceptible enterococcus faecalis under linezolid treatment in a severe polytrauma patient. | 2013 | 24222608 | |
| comparison of bacteria isolated from emergency department patients versus hospitalized patients. | the frequencies and corresponding susceptibilities of bacteria isolated from patients in the emergency department (ed) were compared with those from hospitalized patients. | 2013 | 24249762 |
| dual defensin strategy for targeting enterococcus faecalis. | 2013 | 24284170 | |
| design, synthesis and biological evaluation of α-substituted isonipecotic acid benzothiazole analogues as potent bacterial type ii topoisomerase inhibitors. | the discovery and optimisation of a new class of benzothiazole small molecules that inhibit bacterial dna gyrase and topoisomerase iv are described. antibacterial properties have been demonstrated by activity against dna gyrase atpase and potent activity against staphylococcus aureus, enterococcus faecalis, streptococcus pyogenes and haemophilus influenzae. further refinements to the scaffold designed to enhance drug-likeness included analogues bearing an α-substituent to the carboxylic acid gro ... | 2013 | 24239017 |
| [expression of quorum-sensing related genes during enterococcus faecalis biofilm formation]. | to investigate the relationship between the expression of the quorum-sensing related genes during enterococcus faecalis(ef) biofilm formation. | 2013 | 24238415 |
| characterization of enterococcus faecalis phage ime-ef1 and its endolysin. | enterococcus faecalis is increasingly becoming an important nosocomial infection opportunistic pathogen. e. faecalis can easily obtain drug resistance, making it difficult to be controlled in clinical settings. using bacteriophage as an alternative treatment to drug-resistant bacteria has been revitalized recently, especially for fighting drug-resistant bacteria. in this research, an e. faecalis bacteriophage named ime-ef1 was isolated from hospital sewage. whole genomic sequence analysis demons ... | 2013 | 24236180 |
| bacteriocin protein bacl1 of enterococcus faecalis is a peptidoglycan d-isoglutamyl-l-lysine endopeptidase. | enterococcus faecalis strains are commensal bacteria in humans and other animals, and they are also the causative agent of opportunistic infectious diseases. bacteriocin 41 (bac41) is produced by certain e. faecalis clinical isolates, and it is active against other e. faecalis strains. our genetic analyses demonstrated that the extracellular products of the bacl1 and baca genes, which are encoded in the bac41 operon, coordinately express the bacteriocin activity against e. faecalis. in this stud ... | 2013 | 24235140 |
| role of intracellular stochasticity in biofilm growth. insights from population balance modeling. | there is increasing recognition that stochasticity involved in gene regulatory processes may help cells enhance the signal or synchronize expression for a group of genes. thus the validity of the traditional deterministic approach to modeling the foregoing processes cannot be without exception. in this study, we identify a frequently encountered situation, i.e., the biofilm, which has in the past been persistently investigated with intracellular deterministic models in the literature. we show in ... | 2013 | 24232571 |
| false-positive pneumococcal antigen test in a case of enterococcus faecalis meningitis. | 2013 | 24231250 | |
| antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide against enterococcus faecalis biofilm. | the enterococcus faecalis bacteria have been identified as the most commonly recovered species from teeth with persistent endodontic infections. the antimicrobial activity of essential oils and chloroform (chl), alone and in association with various concentrations of cetrimide (ctr), against biofilm of enterococcus faecalis was investigated. solutions of chl, eucalyptus oil (eo) and orange oil (oo) associated with ctr at 0.3%, 0.2%, 0.1%, and 0.05% were used to determine antimicrobial activity b ... | 2013 | 24265917 |
| a proposal for source tracking of fecal pollution in recreational waters by pulsed-field gel electrophoresis. | this study aimed to identify specific river sources of fecal contamination by applying pulsed-field gel electrophoresis (pfge) to environmental water samples from a recreational beach in japan. the genotypes of all enterococcus faecium and enterococcus faecalis strains used as indicators of fecal pollution on the recreational beach and rivers were analyzed by pfge, and the pfge profiles of the strains were classified at a 0.9 similarity level using dendrogram analysis. pfge types of e. faecium i ... | 2013 | 24256972 |
| molecular characterization of vancomycin-resistant enterococci in serbia: intensive care unit as the source. | the purpose of this study was to evaluate the molecular relatedness of clinical isolates of vancomycin-resistant enterococci (vre) collected from patients of the clinic for infectious and tropical diseases in belgrade. among 40 isolates available for the investigation, 36 were identified as enterococcus faecium, whereas 2 were enterococcus faecalis and enterococcus raffinosus, respectively. pulsed-field gel electrophoresis (pfge) typing revealed 21 strain types, comprising 7 clusters which conta ... | 2013 | 24292087 |
| enterococcus faecalis subverts and invades the host urothelium in patients with chronic urinary tract infection. | bacterial urinary tract infections (uti) are a major growing concern worldwide. uropathogenic escherichia coli has been shown to invade the urothelium during acute uti in mice and humans, forming intracellular reservoirs that can evade antibiotics and the immune response, allowing recurrence at a later date. other bacterial species, such as staphylococcus saprophyticus, klebsiella pneumonia and salmonella enterica have also been shown to be invasive in acute uti. however, the role of intracellul ... | 2013 | 24363814 |
| surgical site infections among high-risk patients in clean-contaminated head and neck reconstructive surgery: concordance with preoperative oral flora. | salivary contamination of surgical wounds in clean-contaminated head and neck surgery with free flap reconstruction remains a major cause of infection and leads to significant morbidity. this study investigates the correlation between intraoral flora and surgical site infections (ssis) among high-risk head and neck cancer patients undergoing resection and free flap reconstruction. | 2013 | 24284742 |
| biofilm formation on polystyrene under different temperatures by antibiotic resistant enterococcus faecalis and enterococcus faecium isolated from food. | the ability of antibiotic resistant e. faecalis and e. faecium isolated from food to form biofilm at different temperatures in the absence or presence of 0.75% glucose was evaluated. a synergistic effect on biofilm at 10 °c, 28 °c, 37 °c and 45 °c and glucose was observed for e. faecalis and e. faecium. | 2013 | 24294231 |
| low metabolic activity of biofilm formed by enterococcus faecalis isolated from healthy humans and wild mallards (anas platyrhynchos). | it is widely known that enterococcus faecalis virulence is related to its biofilm formation. although enterococci are common commensal organisms of the gastrointestinal tract, the difference between commensal and pathogen strains remain unclear. in this study, we compare the biochemical profile of the biofilms formed by two groups of medical and two groups of commensal strains. the medical strains were isolated as pathogens from infections of urinary tract and other infections (wounds, pus and b ... | 2013 | 24294189 |
| prevalence and predictors of urinary tract infections among children with cerebral palsy in makurdi, nigeria. | background. children with cerebral palsy (cp) are prone to urinary tract infection (uti). methods/objectives. the prevalence and the predictors of uti among children with cp were compared to age- and sex-matched children without cp at federal medical centre, makurdi, nigeria, from december 2011 to may 2013. results. the age range was between 2 and 15 years with a mean age of 8.63 ± 3.83 years including 30 males and 22 females. uti was confirmed in 20 (38.5%) cp children compared to 2 children (3 ... | 2013 | 24371524 |
| occurrence, genetic diversity, and persistence of enterococci in a lake superior watershed. | in 2012, the u.s. epa suggested that coastal and great lakes states adopt enterococci as an alternative indicator for the monitoring of recreational water quality. limited information, however, is available about the presence and persistence of enterococci in lake superior. in this study, the density, species composition, and persistence of enterococci in sand, sediment, water, and soil samples were examined at two sites in a lake superior watershed from may to september over a 2-year period. th ... | 2013 | 23455345 |
| effect of high-intensity focused ultrasound on enterococcus faecalis planktonic suspensions and biofilms. | in this study, the effect of high-intensity focused ultrasound (hifu) on enterococcus faecalis on both planktonic suspensions and biofilms was investigated. e. faecalis persist in secondary dental infections as biofilms. glass-bottom petri dishes with biofilms were centered at the focal point of the hifu wave generated by a 250-khz transducer. specimens were subjected to hifu exposure at different periods of 30, 60 and 120 s. the viable bacteria, removal effect and bacterial viability of biofilm ... | 2013 | 23453374 |
| topical linezolid 0.2% for the treatment of vancomycin-resistant or vancomycin-intolerant gram-positive bacterial keratitis. | to report the successful use of topical linezolid 0.2% in the treatment of gram-positive bacterial keratitis. | 2013 | 23453280 |
| [microbial and pharmacological characteristics of vancogenx]. | prosthetic joint infection is a feared complication in total hip arthroplasty. the use of antibiotic-impregnated bone cement is the important part of preventive and therapeutic strategies. at present a number of commercial bone cements are available and support of their use by the results of experimental trials and clinical studies has varied. in relation to this issue we studied essential microbiological and pharmacological characteristics of vancogenx in comparison with gentamicin-loaded bone ... | 2013 | 23452425 |
| [detection of virulence genes in aminoglycoside susceptible and resistant enterococcus faecalis]. | enterococcus spp. is an important cause of nosocomial infections a number of virulence factors that may enhance its ability to colonize have been described. enterococcus is capable of acquiring resistance genes, including high-level resistance (hlr) to aminoglycoside antibiotics. | 2013 | 23450405 |
| virulence, bacterocin genes and antibacterial susceptibility in enterococcus faecalis strains isolated from water wells for human consumption. | the objectives of this study were to detect genes for virulence and bacteriocins in addition to studying the antimicrobial susceptibility of 78 strains of e. faecalis isolated from water wells for human consumption. the virulence and bacteriocin genes of 78 e. faecalis were amplified by pcr and visualized in agarose gels. the antimicrobial susceptibility was determined through diffusion agar tests and the mic through microdilution. it was observed that the major percentage of virulence genes in ... | 2013 | 23449751 |
| engineering of a functional thermostable kanamycin resistance marker for use in moorella thermoacetica atcc39073. | a transformation system for moorella thermoacetica atcc39073 was developed using thermostable kanamycin resistant gene (kanr) derived from the plasmid pjh1 that streptococcus faecalis harbored. when kanr with its native promoter was introduced into uracil auxotrophic mutant of m. thermoacetica atcc39073 together with a gene to complement the uracil auxotrophy as a selection marker, it did not give kanamycin resistance due to poor transcription level of kanr. however, the use of glyceraldehyde-3- ... | 2013 | 23448690 |