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[influence of the lithiasic surface on the strategy: complications and results in the treatment of kidney calculi].stone mass is a fundamental parameter that determines the approach to reno-ureteral lithiasis. reference to stone size based on a single diameter gives us an imprecise idea about stone volume. for this reason, we recommend using the term "stone surface" to express stone mass. stone surface is obtained by multiplying the longitudinal by the transversal diameter expressed in millimeters, using the plain film for radiopaque and the urogram for radiolucent calculi. stone surface is a parameter which ...20072699547
preventing the kidney disease of diabetes mellitus: public health perspectives. 20072643304
rating of the lesions in senile dementia of the alzheimer type: concordance between laboratories. a european multicenter study under the auspices of eurage.the study reported was intended to compare the impressions and analyses of investigators from 11 different laboratories on 2 slides, each from 6 cases with varying quantities of neuropathological change of the type found in alzheimer's disease and normal ageing. the material came from 6 selected female patients over 75 years of age all of whom had been examined in detail and assessed by the blessed test score. two were severely demented, 2 mildly demented and 2 were considered to be normal. unst ...20061698217
neonatal nurse practitioners--a view from perfidious albion? 20061586193
proceedings of the 3rd north sea conference on periodontology. maastricht, the netherlands, 16-19 may 1990. 20061679767
minority student nurses' perceptions of their educational program. 20061773054
rediscovering wormwood: qinghaosu for malaria. 20061347342
study of acute intraoperative suprachoroidal hemorrhage.acute intraoperative suprachoroidal hemorrhage or effusion (aish) was studied in a prospective manner over the past three years. in a general cataract surgery population of 2,523 patient eyes, 15 (0.6%) experienced an aish. results of a specific surgical management technique are presented. surgical control of the aish consisted of immediate closure of the incision and application of pressure directly to the eye. this tamponades the effusion or hemorrhage, which allows coagulation and completion ...20061403754
acute myocardial infarction after blunt chest trauma in young people: need for prompt intervention.we describe four cases of acute myocardial infarction in young patients, secondary to blunt chest trauma. one case was treated with intracoronary thrombolysis and angioplasty, two cases received systemic thrombolysis, and the last one did not have any reperfusion therapy. the coronary angiograms of the left anterior descending artery showed thrombosis in two cases, coronary dissection in one case, and no morphological lesions in the other. we encourage the early performance of angiographic studi ...20061764738
[a case of idiopathic aseptic osteonecrosis of the patellae: repeat study of tc-99m hmdp bone scintigraphy in a reparative phase].a 37-year-old woman entered our hospital because of bilateral knee pain. tc-99m hmdp bone scintigram demonstrated increased activity in the bilateral patellae and cold lesion in the center and left lateral segments of the left patellae, so-called doughnut pattern. trephine biopsy was performed to prove bilateral idiopathic asepct osteonecrosis of the patellae. the knee pain subsided and a bone scan 5 months later demonstrated increased activity in the bilateral patellae more widely than initial ...20061800777
[a case of idiopathic aseptic osteonecrosis of the patellae: repeat study of tc-99m hmdp bone scintigraphy in a reparative phase].a 37-year-old woman entered our hospital because of bilateral knee pain. tc-99m hmdp bone scintigram demonstrated increased activity in the bilateral patellae and cold lesion in the center and left lateral segments of the left patellae, so-called doughnut pattern. trephine biopsy was performed to prove bilateral idiopathic asepct osteonecrosis of the patellae. the knee pain subsided and a bone scan 5 months later demonstrated increased activity in the bilateral patellae more widely than initial ...20061800777
carbohydrate specificity of the receptor sites of mistletoe toxic lectin-i.the carbohydrate specificity of mistletoe toxic lectin-i (ml-i) was studied by haemagglutination-inhibition assay. the results indicated that ml-i has a broad range of affinity for gal alpha,beta linked sequences. the galabiose (e, gal alpha 1----4gal) sequence, a receptor of the uropathogenic e. coli ligand, was one of the best disaccharide inhibitors tested. the lectin also exhibits affinity for lac(gal beta 1----4glc), t(gal beta 1----3galnac), i/ii(gal beta 1----3/4glcnac) and b(gal alpha 1- ...20061525184
"raynaud's disease" in migraineurs: one entity or two?two cases are reported of patients who were diagnosed as having both migraine and raynaud's disease. because they were able to achieve effective control of both headaches and extremity temperature after undergoing a comprehensive approach--utilizing medication, biofeedback, and psychotherapy--it is suggested that both conditions are manifestations of the same disorder. it is recommended that a first step in approaching concurrent diagnoses of this type involve combined treatment as is often used ...20061446993
milk--a better drink? relationships with total serum cholesterol in a cross-sectional survey. the nordland health study.cross-sectional studies have not consistently shown a positive association between milk drinking and serum cholesterol. we studied this relationship in a cohort of 7506 men and women aged 40-42 years in the county of nordland in northern norway (72% of all subjects in the age-bracket living in the county). we found a positive relationship between the percentage of fat in the milk and total serum cholesterol, but no positive relationship between whole fat milk consumption (number of glasses per d ...20061475646
laboratory maintenance of fusobacteria.this unit describes routine laboratory handling of fusobacteria. different media that can be used to grow or enrich fusobacterium nucleatum and other species of this genus are described. the growth and stock conditions as well as the susceptibility of f. nucleatum to oxygen in a pure culture are also discussed.200618770577
immediate effect of instrumentation on the subgingival microflora in deep inflamed pockets under strict plaque control.to investigate (1) reduction in the number of microorganisms obtained directly after subgingival instrumentation, (2) rate of bacterial re-colonization during 2 weeks, under supragingival plaque-free conditions.200616367855
fusobacterium nucleatum transports noninvasive streptococcus cristatus into human epithelial cells.analysis of human buccal epithelial cells frequently reveals an intracellular polymicrobial consortium of bacteria. although several oral bacteria have been demonstrated to invade cultured epithelial cells, several others appear unable to internalize. we hypothesized that normally noninvasive bacteria may gain entry into epithelial cells via adhesion to invasive bacteria. fusobacterium nucleatum is capable of binding to and invading oral epithelial cells. by contrast, streptococcus cristatus bin ...200616369022
porphyromonas gingivalis heat shock protein vaccine reduces the alveolar bone loss induced by multiple periodontopathogenic bacteria.heat shock protein (hsp) can be utilized as a vaccine to cross-protect against multiple pathogenic species. the present study was performed to evaluate porphyromonas gingivalis heat shock protein 60 (hsp60) as a vaccine candidate to inhibit multiple bacteria-induced alveolar bone loss.200616409250
antimicrobial efficacy of medicated root canal filling materials.bacteria remaining within the root canal system are a significant factor in endodontic failures. the aim of this in vitro study was to evaluate the antimicrobial efficacy of commercially available gutta-percha formulations against common endodontic pathogens. standard, tetracycline, or iodoform (also called medicated gutta-percha; mgp) containing gutta-percha were tested against actinomyces israelii, a. naeslundii, enterococcus faecalis, and fusobacterium nucleatum using the agar diffusion metho ...200616427466
genotypic and phenotypic characterization of fusobacteria from chinese and european patients with inflammatory periodontal diseases.phylogenetic and antigenic studies were performed on 48 human oral fusobacterium strains from chinese patients with either necrotizing ulcerative gingivitis (nug) or gingivitis and on 23 fusobacterium nucleatum or fusobacterium periodonticum strains from european periodontitis patients. alignment of partial 16s rrna gene sequences resulted in a phylogenetic tree that corresponded well with the current classification of oral fusobacteria into f. periodonticum and several subspecies of f. nucleatu ...200616464693
molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections.the purpose of the present study was to use terminal restriction fragment length polymorphism analysis and the 16s rrna gene clone library to investigate the diversity of the microbiota associated with asymptomatic and symptomatic endodontic infections and to compare the bacterial community structure in these two clinical conditions. samples were taken from asymptomatic endodontic infections associated with chronic periradicular lesions and from symptomatic infections clinically diagnosed as acu ...200616476021
a sonographic short cervix as the only clinical manifestation of intra-amniotic infection.a sonographically short cervix is a powerful predictor of spontaneous preterm delivery. however, the etiology and optimal management of a patient with a short cervix in the mid-trimester of pregnancy remain uncertain. microbial invasion of the amniotic cavity (miac) and intra-amniotic inflammation are frequently present in patients with spontaneous preterm labor or acute cervical insufficiency. this study was conducted to determine the rate of miac and intra-amniotic inflammation in patients wit ...200616489881
inhibitory effect of weissella cibaria isolates on the production of volatile sulphur compounds.the objective of this study was to characterize the inhibitory effects of weissella cibaria isolates on volatile sulphur compounds (vsc) production both in vitro and in vivo.200616489950
the efficacy of povidone-iodine products against periodontopathic bacteria.a total of 8 strains of 6 bacterial species, porphyromonas gingivalis atcc33277 and tdc286, actinobacillus actinomycetemcomitans atcc29523 and jp2, fusobacterium nucleatum no. 2, tannerella forsythensis atcc43937, prevotella intermedia atcc25611 and streptococcus anginosus atcc33397, were treated with povidone-iodine (pvp-i) gargle (pvp-i: 0.47 and 0.23% w/v) or chlorhexidine gluconate (chg) gargle (chg: 0.002% w/v) for 15, 30 or 60 s, after which they were inoculated into various media, culture ...200616490986
anti-inflammatory activity of a high-molecular-weight cranberry fraction on macrophages stimulated by lipopolysaccharides from periodontopathogens.periodontitis is a chronic inflammatory disease affecting oral tissues. the continuous, high production of cytokines by host cells triggered by periodontopathogens is thought to be responsible for the destruction of tooth-supporting tissues. macrophages play a critical role in this host inflammatory response to periodontopathogens. the aim of this study was to investigate the effect of non-dialyzable material prepared from cranberry juice concentrate on the pro-inflammatory cytokine response of ...200616498070
coaggregation of porphyromonas gingivalis and fusobacterium nucleatum pk 1594 is mediated by capsular polysaccharide and lipopolysaccharide.previous reports have shown that coaggregation between porphyromonas gingivalis and fusobacterium nucleatum, two important periodontopathogens, is mediated by a galactoside on the surface of p. gingivalis and a lectin on f. nucleatum. in the present study, purified capsular polysaccharide (cps) and lipopolysaccharide (lps) of p. gingivalis pk 1924 (serotype k5) were found to be able to bind to f. nucleatum cells and to inhibit binding of f. nucleatum to p. gingivalis serotype k5. sugar binding s ...200616499621
pyogenic infections of the central nervous system secondary to dental affections--a report of six cases.pyogenic infections of the central nervous system of dental origin are quite uncommon in industrialized countries. we report six cases with intracerebral (n = 4) and intraspinal (n = 2) infections treated in our hospital. the microbial pathogen was successfully isolated in all patients. fusobacterium nucleatum as well as streptococcus species were found in three cases. bacillus species were identified in two patients. actinomyces was the etiologic agent in one case. all patients suffered from de ...200616501929
the microbiological origin of fluorescence observed in plaque on dentures during qlf analysis.the aim of this study was to determine the microbiological origin of plaque fluorescence observed during quantitative light-induced fluorescence (qlf) analysis. plaque was sampled from dentures, because of easy accessibility and the homogeneous background provided by the denture tooth during imaging, and the acknowledged comparability to occlusal plaque. forty removable poly(methyl methacrylate) dentures were screened for the presence of fluorescent plaque deposits during qlf analysis. dentures ...200616508267
effects of streptococcus mutans gtfc deficiency on mixed oral biofilms in vitro.the aim of this study was to examine the influence of glucosyltransferase-gene-negative (gtf-) streptococcus mutans strains unable to synthesize water-insoluble or soluble glucan on the structure and macromolecular diffusion properties of in vitro grown mixed oral biofilms. biofilms modeling supragingival plaque consisted of actinomyces naeslundii omz 745, candida albicans omz 110, fusobacterium nucleatum kp-f2, streptococcus oralis sk 248, veillonella dispar atcc 17748t and one of the s. mutans ...200616508276
[lemierre syndrome variant: hepatic abscesses and hepatic venous thrombosis due to fusobacterium nucleatum septicemia].like fusobacterium necrophorum, fusobacterium nucleatum is capable causing lemierre's syndrome. various locations of venous thrombosis have been described associated with fusobacterium sp. septicemia.200616516355
the major outer membrane protein of fusobacterium nucleatum (foma) folds and inserts into lipid bilayers via parallel folding pathways.membrane protein insertion and folding was studied for the major outer membrane protein of fusobacterium nucleatum (foma), which is a voltage-dependent general diffusion porin. the transmembrane domain of foma forms a beta-barrel that is predicted to consist of 14 beta-strands. here, unfolded foma is shown to insert and fold spontaneously and quantitatively into phospholipid bilayers upon dilution of the denaturant urea, which was shown previously only for outer membrane protein a (ompa) of esch ...200616310217
the ability of selected oral microorganisms to emit red fluorescence.some novel caries detection and excavation devices rely on the ability of bacteria to produce red fluorescing compounds. the aim of this study was to examine the ability of selected oral microorganisms to emit red fluorescence. streptococcus mutans, s. oralis, s. salivarius, s. sobrinus, lactobacillus fermentans, l. casei, l. rhamnosus, actinomyces naeslundi, a. israelii, prevotella intermedia, and fusobacterium nucleatum were inoculated onto columbia agar with haemin and vitamin k and incubated ...200616352873
inhibitory effects of macrocarpals on the biological activity of porphyromonas gingivalis and other periodontopathic bacteria.macrocarpals, which are phloroglucinol derivatives contained in eucalyptus leaves, exhibit antimicrobial activity against a variety of bacteria including oral bacteria. this study examined effects of macrocarpals a, b, and c on periodontopathic bacteria, especially porphyromonas gingivalis.200616626372
response of human macrophage-like cells to stimulation by fusobacterium nucleatum ssp. nucleatum lipopolysaccharide.monocytes/macrophages are key members of the innate immune system and are present in higher numbers in active periodontal lesions than in inactive sites. the aim of this study was to characterize the response of human monocyte u937 cells, differentiated into adherent macrophages by treatment with phorbol-12-myristate 13-acetate, to stimulation by fusobacterium nucleatum ssp. nucleatum lipopolysaccharide. attachment of (3)h-lipopolysaccharide to macrophage-like cells was partially inhibited by an ...200616626377
prevalence of periodontopathic bacteria on the tongue dorsum of elderly people.the aim of this study was to analyse the prevalence of oral bacteria on the dorsum of the tongue. in addition, the relationship between the number of teeth and the microflora present on the coating of the tongue in a population of 85-year-old people was assessed.200616677187
selective inhibition of porphyromonas gingivalis growth by a factor xa inhibitor, dx-9065a.porphyromonas gingivalis is a causative bacterium of adult periodontitis. however, there is no drug specific for p. gingivalis and for its virulence factor.200616677284
genetic diversity of oral fusobacterium nucleatum isolated from patients with different clinical conditions.the genetic diversity of 23 oral fusobacterium nucleatum isolated from 15 periodontal patients, eight from seven healthy subjects, nine from nine aids patients and two from two cebus apella monkeys were analyzed. ecori restricted the bacterial dna and 28 ribotypes grouped from a to j groups were obtained. isolates formed 24 ribotypes which were contained into a, b, c, d, e and f groups, and three reference strains and two clinical isolates of a. actinomycetemcomitans, and e. coli cdc formed four ...200616699623
supragingival plaque may modify the effects of subgingival bacteria on attachment loss.bacterial interactions in response to local and systemic factors may explain important features of the pathogenesis of periodontal disease. this study evaluates the role of supragingival plaque level on the relationship between subgingival microorganisms and the risk of attachment loss.200616671872
a model of spontaneous periodontitis in the miniature goat.the pathogenesis and progression of periodontal disease have been extensively studied through the use of animal models. however, no animal model has yet been established that is precisely similar to periodontitis in humans. in the present study, we examined the use of shiba goats as a model for spontaneous periodontitis.200616671878
[application of "microring an" to "level 1": presumptive identification of anaerobic gram-negative bacilli].commonly isolated anaerobic gram-negative rods (4 genus 64 strains), some other important gram-negative anaerobic species (9 genus 45 strains), and cigar-shaped clostridia (11 strains) were studied on their susceptibility patterns to 6 agents on "microring an". some modifications were made in the methods and interpretation of results. susceptibility patterns to erythromycin, rifampicin, colistin, benzylpenicillin, kanamycin, and vancomycin were following (sensitive [s], intermediate [i], resista ...200617822332
characterization of proteolytic activities of fusobacterium nucleatum.this investigation attempted to detect the proteolytic activity of fusobacterium nucleatum in living cells, lysate cells, and supernatant of cultures. the reactions were optimized in their ph, temperature, reaction time, enzyme source, and substrate volume. synthetic substrates beta-naphthylamides (cys-na, ser-na, leu-na, glu-na, lys-na and bana), carbobenzoxy l-tirosine p-nitrophenylester (ctn), and natural substrate azoalbumin were used. reaction occurred with cys-na, ser-na, and glu-na in liv ...200616728241
state-dependent conformations of the translocation pathway in the tyrosine transporter tyt1, a novel neurotransmitter:sodium symporter from fusobacterium nucleatum.the gene of a novel prokaryotic member (tyt1) of the neurotransmitter:sodium symporter (nss) family has been cloned from fusobacterium nucleatum. in contrast to eukaryotic and some prokaryotic nsss, which contain 12 transmembrane domains (tms), tyt1 contains only 11 tms, a characteristic shared by approximately 70% of prokaryotic nss homologues. nonetheless upon heterologous expression in an engineered escherichia coli host, tyt1 catalyzes robust na+-dependent, highly selective l-tyrosine transp ...200616798738
antimicrobial actions of benzimidazoles against the oral anaerobes fusobacterium nucleatum and prevotella intermedia.benzimidazoles are widely used as proton-pump inhibitors to control stomach hyperacidity and have been found also to have antimicrobial actions against helicobacter pylori and oral streptococci. our primary aim was to determine if they are active also against oral anaerobes associated with gingivitis. our major focus was on catabolism because it leads to production of inflammatory metabolites such as butyrate and ammonia. the benzimidazoles are effective in the protonated form at acid ph values ...200616806047
antimicrobial barrier of an in vitro oral epithelial model.oral epithelia function as a microbial barrier and are actively involved in recognizing and responding to bacteria. our goal was to examine a tissue engineered model of buccal epithelium for its response to oral bacteria and proinflammatory cytokines and compare the tissue responses with those of a submerged monolayer cell culture.200616815238
evaluation of 4 swab transport systems for the recovery of atcc and clinical strains with characterized resistance mechanisms.despite limitations of swab transport systems (sts), many clinical samples and bacterial isolates are sent on these devices. we evaluated the performance of 4 commercial sts: m40 transystem (s1, copan italia, bovezzo, italy), uni-ter (s2, meus, piove di sacco, italy), euromed (s3, lab service, surbo, italy), and eurotubo (s4, deltalab, rubí, spain). survival of atcc and clinical strains with characterized resistance mechanisms, stored at room temperature and using the roll-plate method (nccls m4 ...200616822635
effectiveness of ultrasonic instruments in the therapy of severe periodontitis: a comparative clinical-microbiological assessment with curettes.patients with deep periodontal pockets were treated with either vector system (tg) or manual instruments (cg). clinical assessments by supragingival plaque (pl+), gingival index (gi), bleeding on probing (bop), probing depth (pd), clinical attachment level (cal) and subgingival plaque collection for microbiological analysis were made prior to and after treatment. multiplex polymerase chain reaction was used to determine the presence of actinobacillus actinomycetemcomitans, campylobacter rectus, ...200616841550
inflammation, heat shock proteins and periodontal pathogens in atherosclerosis: an immunohistologic study.inflammation is a significant component of atherosclerosis lesions. bacteria, including periodontopathogens, have been demonstrated in atherosclerotic plaques and cross-reactivity of the immune response to bacterial groel with human heat shock protein 60 has been suggested as a link between infections and atherosclerosis.200616842503
an in vitro study of antimicrobial activity and efficacy of iodine-generating hydrogel dressings.to determine the antimicrobial activity and efficacy of different formulations of novel bioxygenating hydrogel dressings (which deliver both iodine and oxygen into the wound) against various target organisms by means of an in vitro test system that more effectively mimics the conditions encountered when dressings are in contact with wounds.200616869198
genetic and biochemical characterization of fus-1 (oxa-85), a narrow-spectrum class d beta-lactamase from fusobacterium nucleatum subsp. polymorphum.previous studies have reported beta-lactamase-mediated penicillin resistance in fusobacterium nucleatum, but no beta-lactamase gene has yet been identified in this species. an f. nucleatum subsp. polymorphum strain resistant to penicillin and amoxicillin was isolated from a human periodontitis sample. dna cloning and sequencing revealed a 765-bp open reading frame encoding a new class d beta-lactamase named fus-1 (oxa-85). a recombinant escherichia coli strain carrying the bla(fus-1) gene exhibi ...200616870757
protective effects of grape seed proanthocyanidins against oxidative stress induced by lipopolysaccharides of periodontopathogens.during phagocytosis or stimulation with bacterial components, macrophages activate various cell processes, including the production of reactive oxygen species (ros) and reactive nitrogen species (rns), which are critical for successful defense against invading organisms. increased levels of ros/rns create oxidative stress that results in tissue and bone destruction. grape seed proanthocyanidins have been reported to possess a wide range of biologic properties against oxidative stress. in the pre ...200616881806
in vitro activity of linezolid against clinical isolates of fusobacterium spp.although most susceptibility studies for linezolid have investigated aerobic bacteria, only a few have investigated anaerobe isolates. the aim of the present study was to determine the antibacterial activity of linezolid against a larger sample of clinical isolates of fusobacterium spp. and to report on the detailed susceptibility, stratified by species.200616905529
expression of the short fimbriae of porphyromonas gingivalis is regulated in oral bacterial consortia.the mfa1 protein of porphyromonas gingivalis is the structural subunit of the short fimbriae and mediates coadhesion between p. gingivalis and streptococcus gordonii. we utilized a promoter-lacz reporter construct to examine the regulation of mfa1 expression in consortia with common oral plaque bacteria. promoter activity of mfa1 was inhibited by s. gordonii, streptococcus sanguinis and streptococcus mitis. in contrast, streptococcus mutans, streptococcus cristatus, actinomyces naeslundii, actin ...200616907740
influence of residual bacteria on periapical tissue healing after chemomechanical treatment and root filling of experimentally infected monkey teeth.the purpose of this study was twofold: first, to determine the influence on the healing of the periapical tissues when selected bacterial strains and combinations thereof remain after root canal treatment; and, second, the relationship to healing of the quality of the root filling. in eight monkeys, 175 root canals, previously infected with combinations of four or five bacterial strains and with radiographically verified apical periodontitis, were endodontically treated, bacteriologically contro ...200616911098
association of eubacterium nodatum and treponema denticola with human periodontitis lesions.the purpose of the present investigation was to compare the levels, proportions and percentage of sites colonized by 40 bacterial species in subgingival plaque samples from periodontally healthy subjects and patients with chronic periodontitis to seek possible pathogens other than the consensus pathogens porphyromonas gingivalis and tannerella forsythia.200616922925
oral adhesion and survival of probiotic and other lactobacilli and bifidobacteria in vitro.most probiotic products are consumed orally and hence it is feasible that the bacteria in these products may also attach to oral surfaces; however, the effects of these bacteria on the oral ecosystem are mostly unknown. our aim was to evaluate the oral colonization potential of different probiotic, dairy, and fecal lactobacillus and bifidobacterium strains in vitro.200616922933
antibacterial effect of electrolyzed water on oral bacteria.this study investigated the antibacterial effect of electrolyzed water on oral bacteria both in vitro and in vivo. tap water was electrolyzed in a water vessel using platinum cell technology. the electrolyzed tap water (called puri-water) was put in contact with five major periodontopathogens or toothbrushes contaminated with these bacteria for 30 sec. in addition, puri-water was used as a mouthwash for 30 sec in 16 subjects and the antibacterial effect on salivary bacteria was evaluated. puri-w ...200616953177
periodontal therapy in siblings with papillon-lefèvre syndrome and tinea capitis: a report of two cases.report of clinical and microbiological periodontal findings before and 6 months after treatment of two siblings with papillon-lefèvre syndrome (pls) and tinea capitis.200616970621
coaggregation interactions between oral and endodontic enterococcus faecalis and bacterial species isolated from persistent apical periodontitis.interactions between enterococcus faecalis and other species found in root canal infections might be important for the development and persistence of periapical disease. the aim of this study was to investigate the coaggregation interactions between e. faecalis clinical isolates and species previously shown to survive and induce apical periodontitis in monkeys: peptostreptococcus anaerobius, prevotella oralis, fusobacterium nucleatum, and streptococcus anginosus. intergeneric coaggregation assay ...200616982270
photodynamic therapy for endodontic disinfection.the aims of this study were to investigate the effects of photodynamic therapy (pdt) on endodontic pathogens in planktonic phase as well as on enterococcus faecalis biofilms in experimentally infected root canals of extracted teeth. strains of microorganisms were sensitized with methylene blue (25 microg/ml) for 5 minutes followed by exposure to red light of 665 nm with an energy fluence of 30 j/cm2. methylene blue fully eliminated all bacterial species with the exception of e. faecalis (53% kil ...200616982278
in vitro antimicrobial activity of sodium hypochlorite and chlorhexidine against selected single-species biofilms.to investigate the antimicrobial activity of 2.5% and 5.25% sodium hypochlorite and 2.0% chlorhexidine gel and liquid as endodontic-irrigating substances against selected single-species biofilms.200617014526
splenic vein thrombosis presenting as fusobacterium nucleatum bacteremia. 200617022361
[multiple intracerebral lesions. identification of the causative agent by 16s rdna-pcr].molecular biological methods such as polymerase chain reaction (pcr) enable microbiologists to detect bacteria even if antibiotic treatment has already been started. based on this case report of a 39-year-old man with multiple intracerebral lesions, we describe a pcr method called 16s rdna-pcr which can be used to identify panbacterial dna by focussing on the universal gene sequences for the bacterial 16s part of the ribosome.200617063323
carpegen real-time polymerase chain reaction vs. anaerobic culture for periodontal pathogen identification.the aim of this study was to compare two methods of microbiological diagnosis, anaerobic bacterial culture and real-time polymerase chain reaction (pcr), for the detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythia, fusobacterium nucleatum, and treponema denticola.200617064390
amphipathic polymers: tools to fold integral membrane proteins to their active form.among the major obstacles to pharmacological and structural studies of integral membrane proteins (mps) are their natural scarcity and the difficulty in overproducing them in their native form. mps can be overexpressed in the non-native state as inclusion bodies, but inducing them to achieve their functional three-dimensional structure has proven to be a major challenge. we describe here the use of an amphipathic polymer, amphipol a8-35, as a novel environment that allows both beta-barrel and al ...200617115690
effects of sonicated prevotella intermedia, fusobacterium nucleatum and lactobacillus casei extracts on interleukin-8 production by human dental pulp cells.the objective of this study was to determine the effects of prevotella intermedia, fusobacterium nucleatum and lactobacillus casei on the production of il-8 by human dental pulp cells. human dental pulp cells from teeth of young patients (aged 18-25 years) were cultured and tested with sonicated p. intermedia atcc 25611, f. nucleatum atcc 25586 and l. casei atcc 4646 extracts. il-8 secreted into the culture supernatants were measured at 6, 12 and 24 hours using a quantitative sandwich enzyme imm ...200617120973
common antacids could help keep gingivitis at bay. 200617128225
antimicrobial effect of acidified nitrate and nitrite on six common oral pathogens in vitro.salivary nitrate is positively correlated with plasma nitrate and its level is 9 times the plasma level after nitrate loading. nitrate in saliva is known to be reduced to nitrite by oral bacteria. nitrate and nitrite levels in saliva are 3 - 5 times those in serum in physiological conditions respectively in our previous study. the biological functions of high salivary nitrate and nitrite are still not well understood. the aim of this in vitro study was to investigate the antimicrobial effects of ...200617134590
crystallization and preliminary x-ray data of the fada adhesin from fusobacterium nucleatum.fusobacterium nucleatum is a gram-negative anaerobe prevalent in the oral cavity that is associated with periodontal disease, preterm birth and infections in other parts of the human body. the bacteria attach to and invade epithelial and endothelial cells in the gum tissue and elsewhere via a 13.7 kda adhesin protein fada (fusobacterium adhesin a). fada exists in two forms: the intact form (pre-fada), consisting of 129 amino acids, and the mature form (mfada), which lacks an 18-residue signal se ...200617142900
efficient gene transfer and targeted mutagenesis in fusobacterium nucleatum.fusobacterium nucleatum is a gram-negative anaerobe important in dental biofilm ecology and infectious diseases with significant societal impact. the lack of efficient genetic systems has hampered molecular analyses in this microorganism. we previously reported construction of a shuttle plasmid, phs17, using the native fusobacterial plasmid pfn1 and an erythromycin resistance cassette. however, the host range of phs17 was restricted to f. nucleatum, atcc 10953, and the transformation efficiency ...200616115683
antibacterial action of photoactivated disinfection {pad} used on endodontic bacteria in planktonic suspension and in artificial and human root canals.to measure antibacterial action of photoactivated disinfection (pad) on endodontic bacteria in planktonic suspension and root canals. methods: four bacteria, fusobacterium nucleatum,peptostreptococcus micros, prevotella intermedia and streptococcus intermedius, were tested in suspension. after mixing equal volumes of tolonium chloride and bacterial suspension for 60s, each 200 microl of concentration (>10(6)cfu ml(-1)) was irradiated with light at 633+/-2 nm. each energy dose/tolonium chloride c ...200616239058
infection and infectious diseases. 20061796972
infection and infectious diseases. 20061796972
temporary pacemakers. 20061594873
analysis of the incubation period for measles in the epidemic in greenland in 1951 using a variance components model.this paper presents a model for analysing the incubation period of highly infectious diseases in populations where almost all susceptibles become infected during an epidemic. the model leads to a simple method for estimating the variance of the duration of the incubation period without any distributional assumptions. further, the influence of covariates on the duration of the incubation period can be analysed. data from the epidemic of measles in greenland in 1951 are analysed and it is found th ...20061594801
somatic reversion/suppression in duchenne muscular dystrophy (dmd): evidence supporting a frame-restoring mechanism in rare dystrophin-positive fibers.many duchenne muscular dystrophy (dmd) patients are known to have rare staining dystrophin-positive fibers, termed "revertants." the precise etiology of these rare fibers is unknown. the most likely explanation, however, is somatic mosaicism or somatic reversion/suppression. immunocytochemistry was performed on serial sections from deleted and nondeleted patients, with a panel of antibodies--9219, 1377, 9218, and dys-2--that span dystrophin. both familial and nonfamilial patients possessed rever ...20061570844
[perversions in 1991]. 20061594857
iron, transferrin, and haptoglobin levels after a single bout of exercise in men.twelve, healthy male university student volunteers, between the ages of 20 and 23, were studied. all subjects were considered normal after cardiopulmonary and electrocardiographic examination. the maximal aerobic capacity (vo2 max) of each subject was determined. the exercise programmes were performed on a mechanically braked monark cycle ergometer. the subjects were required to perform the three tests, one per week. each subject had a catheter inserted in an antecubital vein and blood samples w ...20061594669
the educational process at the mount sinai school of medicine: the first 25 years.although the charter for the school of medicine was signed in 1963, and active recruitment of faculty commenced almost immediately, the recruitment of students, in fact, did not begin until several years later, with admission of a first-year and a third-year class in september 1968. from that time to the present, the class size has increased from a small, carefully selected group of 36 first-year students to its current size of 115 students in each of the basic science years and 132 students in ...20062685587
[nsf's elected representative to elite series. interview by bjørn arild ostby]. 20061797065
facial paralysis reconstruction with gore-tex soft-tissue patch.no procedure perfectly restores a face paralyzed by surgical ablation of the facial nerve. although dynamic reconstructive procedures are preferred for treating patients with complete facial paralysis, various conditions contraindicate their use, making static suspension of the paralyzed face a reasonable surgical alternative. expanded polytef soft-tissue patch (polytetrafluoroethylene; gore-tex soft-tissue patch, gore-tex, w. l. gore & assoc inc., flagstaff, ariz) was used to help correct midfa ...20061418899
endotoxin antibody for sepsis in infants. 20061347362
effect of the vibratory acoustic stimulation on fetal heart rate patterns of premature fetuses.the purpose of this study was to examine the heart rate patterns before and after a standardized external vibratory acoustic stimulation in a group of 24 healthy premature fetuses at 32-35 weeks gestational age. fhr was analysed on line by sonicaid computer system 8000. a significant increase in the number of accelerations and an increase of variation after stimulus were observed. all other fhr patterns such as baseline, high and low episodes did not change significantly.20061346598
thymocytopoiesis is maintained by blood-borne precursors throughout postnatal life. a study in parabiotic mice.this study was designed to resolve the long standing controversy as to whether hematogenous precursors or resident intrathymic precursors maintain thymocytopoiesis in postnatal life. the kinetics of thymic chimerism was examined over a 21-wk period in 105 pairs of nonirradiated, thy-1-alloantigen-disparate, parabiotic b10.s mice. thymic chimerism reached reached maximum levels of 25% in the thy-1b and 16% in the thy-1a partners (mean 20.5% and 9.8%, respectively) 6 to 7 wk after parabiotic union ...20061347301
ec directives on medical devices. 20061392944
[transverse myelitis and optic neuromyelitis in children. apropos of 3 case reports].we report on three children with acute transverse myelitis. one of them also had optic neuritis. in connection with these three cases, we discuss etiology, diagnosis and outcome of transverse myelitis in childhood and possible involvement of the optic nerve. our observations show the variability of spinal cord deficit and the fact that there can be a good prognosis, despite severe initial neurological deficits and massive elevation of basic myelin protein in children with atm.20061594852
comparison of the long-term, postsurgical survival of women and men in the coronary artery surgery study (cass).this study compares the survival of men and women an average of 6 years after coronary artery bypass graft surgery (cabg) by means of the coronary artery surgery study (cass) registry. subjects included in these analyses were the 6100 men and 1097 women who survived surgery. medical history and physical and laboratory information were collected from each patient at baseline. men and women were compared for differences in baseline characteristics, long-term survival (by means of the cox proportio ...20062643286
[differential diagnosis of 2 forms of necrospermia]. 20063321663
thrombocytopenia and the neglected megakaryocyte. 20061435936
the molecular genetics of schizophrenia. 20061393109
comparative studies on the mode of action of proctolin and phorbol-12,13-dibutyrate in their ability to contract the locust mandibular closer muscle.the role of proctolin has been further investigated in the locust (locusta migratoria) mandibular closer muscles. radioactive calcium uptake measurements were made using protease-dissociated muscle cells. both the phorbol ester, phorbol-12,13-dibutyrate, and proctolin produce tonic contractions which are associated with the influx of extracellular calcium. the thresholds for proctolin and the phorbol ester to contract the muscle were 1-10 nm and 10-100nm, respectively, while their respective thr ...20061360273
tissue engineering. keystone symposia on molecular and cellular biology. april 3-10, 1992. abstracts. 20061351964
nerve sheath myxoma: an immunohistochemical study of a case. 20061489420
mandatory testing? 20061426457
nih's first strategic plan debated. 20061538737
adhesion molecules (n-cam and tenascin) in embryonic development and tissue regeneration. 20061698808
from the beginning: an historical review of medical art. 20062691550
[hypertension detection and follow-up program(hdfp)]. 20061635190
[cornea bank: an increase in the number of transplantations is possible]. 20061636297
[fecal incontinence. epidemiology, diagnosis and treatment]. 20061563608
doppler investigation of uteroplacental blood flow resistance in the second trimester: a screening study for pre-eclampsia and intrauterine growth retardation. 20061472202
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