Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| an aeromonas salmonicida type iv pilin is required for virulence in rainbow trout oncorhynchus mykiss. | aeromonas salmonicida expresses a large number of proven and suspected virulence factors including bacterial surface proteins, extracellular degradative enzymes, and toxins. we report the isolation and characterization of a 4-gene cluster, tapabcd, from virulent a. salmonicida a450 that encodes proteins homologous to components required for type iv pilus biogenesis. one gene, tapa, encodes a protein with high homology to type iv pilus subunit proteins from many gram-negative bacterial pathogens, ... | 2002 | 12240967 |
| necrotising fasciitis caused by vibrio vulnificus in a child without known risk factors. | 2002 | 12269260 | |
| infectious outbreaks associated with bivalve shellfish consumption: a worldwide perspective. | outbreaks of shellfish-associated infection have been reported for more than a century. since the early 1970s, the global consumption of shellfish has increased considerably--and with it, the reports of outbreaks of infection. most of these reports have originated from the united states, but europe and, to a lesser extent, asia and australia have also been represented. the majority of outbreaks have been linked to oysters, followed by clams and mussels. hepatitis a virus caused the largest ever ... | 2002 | 12355378 |
| promoter analysis and regulatory characteristics of vvhba encoding cytolytic hemolysin of vibrio vulnificus. | cytolytic hemolysin, a gene product of vvha, is a putative virulence factor of the pathogenic bacterium vibrio vulnificus. we have previously shown that hemolysin production is repressed by adding glucose to culture media and that production can be restored by adding camp. in this study, hemolysin activity and the level of vvh transcript were determined to reach a maximum in late exponential phase and were repressed when cells entered stationary phase. northern blot and primer extension analyses ... | 2002 | 12356775 |
| phage therapy of local and systemic disease caused by vibrio vulnificus in iron-dextran-treated mice. | vibrio vulnificus is a gram-negative bacterium that contaminates filter-feeding shellfish such as oysters. after ingestion of contaminated oysters, predisposed people may experience highly lethal septicemia. contamination of wounds with the bacteria can result in devastating necrotizing fasciitis, which can progress to septicemia. the extremely rapid progression of these diseases can render antibiotic treatment ineffective, and death is a frequent outcome. in this study, we examined the potentia ... | 2002 | 12379704 |
| in vitro and in vivo activities of newer fluoroquinolones against vibrio vulnificus. | the mics of six fluoroquinolones as well as minocycline and cefotaxime for 46 clinical isolates of vibrio vulnificus were determined by the agar dilution method. all the drugs tested had good activities against all isolates, with the mics at which 90% of the isolates tested were inhibited (mic(90)s) by five of the fluoroquinolones ranging between 0.03 and 0.06 micro g/ml. the mic(90) of lomefloxacin, on the other hand, was 0.12 micro g/ml. time-kill studies were conducted with these agents and a ... | 2002 | 12384368 |
| seasonality of chesapeake bay bacterioplankton species. | bacteria, gamma-subclass of proteobacteria, vibrio-photobacterium, vibrio vulnificus, vibrio cholerae-vibrio mimicus, and vibrio cincinnatiensis in water samples collected from the choptank river in chesapeake bay from 15 april to 16 december 1996 were enumerated using a fluorescent oligonucleotide direct-counting (fodc) procedure. fodc results obtained using a bacteria taxon-specific probe ranged from one-third the number of to the same number as that obtained by the acridine orange direct coun ... | 2002 | 12406742 |
| bacteria of the gamma-subclass proteobacteria associated with zooplankton in chesapeake bay. | the seasonal abundance of gamma-subclass proteobacteria, vibrio-photobacterium, vibrio cholerae-vibrio mimicus, vibrio cincinnatiensis, and vibrio vulnificus in the choptank river of chesapeake bay associated with zooplankton was monitored from april to december 1996. large (>202- microm) and small (64- to 202- microm) size classes of zooplankton were collected, and the bacteria associated with each of the zooplankton size classes were enumerated by fluorescent oligonucleotide direct count. larg ... | 2002 | 12406743 |
| detection of cytotoxin-hemolysin mrna in nonculturable populations of environmental and clinical vibrio vulnificus strains in artificial seawater. | the objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of vibrio vulnificus. for that purpose, we applied seminested reverse transcription-pcr (rt-pcr) to viable but nonculturable (vbnc) populations of v. vulnificus and targeted the cytotoxin-hemolysin virulence gene vvha. three strains, two environmental, if vv10 and if vv18, and one clinical, c7184, were used in this study. artificial seawater, inoculated wi ... | 2002 | 12406760 |
| clinical comparison between glibornuride (glutril) and tolbutamide in maturity-onset diabetes: a controlled double-blind trial. | 2002 | 134878 | |
| vibrio vulnificus cytolysin induces hyperadhesiveness of pulmonary endothelial cells for neutrophils through endothelial p-selectin: a mechanism for pulmonary damage by vibrio vulnificus cytolysin. | vibrio vulnificus cytolysin forms transmembrane pores that are permeable to calcium ions in pulmonary endothelial cells, and has been suggested as an important virulence factor that sequestrate neutrophils primarily in the lung. to elucidate the mechanism we investigated whether the cytolysin affect the expression of endothelial p-selectin and adhesiveness of pulmonary endothelial cells for neutrophils. the cytolysin increased the adhesiveness of cpae cell, a pulmonary endothelial cell line, for ... | 2002 | 12515397 |
| [recurrence in soft tissues of a giant cell tumor of the femoral condyle]. | a case of recurrence with osteogenesis only in the soft tissues of a giant cell tumour is reported. | 2002 | 128069 |
| [the role of the auxilliary health personnel in the prevention of iatrogenic quadriceps contracture]. | 2002 | 120064 | |
| [details of a denounced physician (e.n.t.) suspected of bodily injury, and the following expert judgement (author's transl)]. | an otologist was denounced and the patient's case history was confiscated because after tonsillectomy an absecess and a thrombosis of the vena jugularis interna had developed. the procedure and the medical aspect how at first being judged by the lawyer and the public prosecutor are described. on the base of the given medical opinion the court quashed proceedings. because of the growing number of denouncements this casuistic paper ends with four guiding principles to protect against unjustified m ... | 2002 | 135139 |
| editorial: health care crisis. | 2002 | 124076 | |
| [acid glycosaminoglycans in localized pleural mesothelioma--histochemical and biochemical study]. | 2002 | 135110 | |
| [an invariant of plasma viscosity]. | determination of "m" invariant cannot lead to an absolute measurement of plasmatic viscosity, because "m" increases with temperature. it allows however to compare data determined in various conditions. | 2002 | 126722 |
| paediatric surgeons are necessary. | 2001 | 93224 | |
| anticonvulsant action of a 1,5-benzodiazepine, clobazam, in reflex epilepsy. | clobazam, an anxiolytic 1,5-benzodiazepine, has been evaluated as an anticonvulsant in 2 animal models. in mice showing sound induced seizures, clobazam, 1--4 mg/kg, i.p., blocked seizure responses for 1--2 hr. in senegalese baboons papio papio showing photically induced myoclonus or seizures, clobazam, 2--12 mg/kg, i.v., totally prevented such responses for up to 6 hr. in baboons pretreated with allylglycine, 170--185 mg/kg, a similar but briefer protection was induced by clobazam. neurological ... | 2001 | 98317 |
| consistency of autoradiographic dna repair in cells treated "in vitro" with n-diazoacetylglycine amide. a preliminary approach to quantitate the absolute amount of dna repair. | 2001 | 98772 | |
| [medical management of tachycardias (author's transl)]. | guidelines for a step-wise plan of treatment of tachycardias have been compiled based on clinical empirical experience and with the aid of surface electrocardiograms, intracardial electrograms and stimulation techniques. the plan is primarily with the aid of surface electrocardiograms, intracardial electrograms and stimulation techniques. the plan is primarily oriented with respect to the antiarrhythmic efficacy, the adverse reactions and the practicability of the respective agents. any type of ... | 2001 | 93075 |
| visual aids in nutrition education. a guide to their preparation and use. | 2001 | 95011 | |
| [volvulus of the afferent loop after billroth ii operation (author's transl)]. | a case report is given of a patient, who had a volvulus of the afferent loop after billroth ii operation; on relaparotomy it was found, that this loop had slipped cranially through the mesocolic slit, twisted, and become gangreneous, due to bad adjustement of the slit. increased levels of serum amylase initially had given rise to the suspicion, that the deteriorating condition of the patient was due to pancreatitis. the patient recovered fully after resection had been done and an y-roux-anastomo ... | 2001 | 94934 |
| ataxia-telangiectasia and hepatocellular carcinoma. | ataxia-telangiectasia (at) is a multi-system disease involving the cerebellum, cutaneous blood vessels and the immune system including both cellular and humoral components. it also involves hematological, endocrine and peripheral nervous systems. this disease is often associated with abnormal liver function tests, such as, raised alkaline phosphatase and various nonspecific histological changes in the liver. high incidence of various malignancies involving lymphoreticular, gastrointestinal and m ... | 2001 | 92892 |
| [surgery of hepatic hydatic cyst. per-operative complications. possibility of anaphylactic etiology (author's transl)]. | among fifty cases hepatic hydatid cysts, we have observed four serious complications during operation. these complications appeared at the time of the puncture of the cysts with a severe shock in three cases. anaphylactic etiology can be suspected but not proved without specific tests. because the severity of these accidents (one death), anesthesiologist must be aware of the possibility of such complications: it is important to recognize anaphylactic reactions during surgical procedure to instit ... | 2001 | 95469 |
| perinatal mortality. | 2001 | 93220 | |
| [diagnostic and therapy of postoperative cardiac low-output-syndrome by means of intraaortic balloon pumping (iabp) or external counterpulsation (ecp)--a comparison (author's transl)]. | a beneficial influence on the myocardial energy imbalance of the hypoxaemically damaged heart can be reached with either intraaortic balloon pumping (iabp) or external counterpulsation (ecp). in the realm of our acquired clinical experience with two groups of patients, the technical demands of these cardiac assist devices are discussed and distinguished from one another in relation to different indications. | 2001 | 1084037 |
| [antigen-specific t-cell factors in the regulation of the immune response (author's transl)]. | we have examined in this brief review several facets of antigen-specific t-cell mediators found independently by different investigators. these factors have many common properties: (1) they are produced as a consequence of antigen stimulation; (2) they are obtained from t-cell populations and not from b cells or macrophages; their synthesis by t cells, however, has not been formally demonstrated; (3) they are antigen-specific and are selectively adsorbed by antigen-sepharose columns; (4) they la ... | 2001 | 95085 |
| antacid increases survival of vibrio vulnificus and vibrio vulnificus phage in a gastrointestinal model. | viable counts of three strains of vibrio vulnificus and its phage were determined during exposure to a mechanical gastrointestinal model with or without antacid for 9 h at 37 degrees c. v. vulnificus was eliminated (>4-log reduction) within 30 min in the gastric compartment (ph decline from 5.0 to 3.5). viable v. vulnificus cells delivered from the gastric compartment during the first 30 min of exposure reached 10(6) to 10(8) cfu/ml in the intestinal compartment after 9 h (ph 7.0). phages were e ... | 2001 | 11425699 |
| [necrotizing dermatitis due to vibrio vulnificus]. | vibrio vulnificus is a gram-negative halophilic pathogen for man that can cause septicemia in patients with chronic liver disease. in healthy subjects, infections are generally local or regional. we report two cases of necrotizing dermo-hypodermitis due to vibrio vulnificus. | 2001 | 11427803 |
| [rapidly progressive and fatal multiple organ failure and necrosis of lower extremities due to vibrio vulnificus sepsis]. | 2001 | 11460358 | |
| purification and characterization of a vulnificolysin-like cytolysin produced by vibrio tubiashii. | an extracellular cytolysin from vibrio tubiashii was purified by sequential hydrophobic interaction chromatography with phenyl-sepharose cl-4b and gel filtration with sephacryl s-200. this protein is sensitive to heat and proteases, is inhibited by cholesterol, and has a molecular weight of 59,000 and an isoelectric point of 5.3. in addition to lysing various erythrocytes, it is cytolytic and/or cytotoxic to chinese hamster ovary cells, caco-2 cells, and atlantic menhaden liver cells in tissue c ... | 2001 | 11472951 |
| vibrio vulnificus septicemia in a patient with the hemochromatosis hfe c282y mutation. | vibrio vulnificus is an extremely invasive gram-negative bacillus found in marine waters that causes overwhelming bacteremia and shock that is associated with high mortality. impaired iron metabolism has been implicated in the susceptibility to v vulnificus bacterial infections. we report a case of fatal v vulnificus sepsis in a 56-year-old man who died within 1 to 3 days after consuming raw seafood. at autopsy, he was found to have micronodular cirrhosis and iron overload. postmortem genetic an ... | 2001 | 11473471 |
| comparison of selective media for the detection of vibrio vulnificus in environmental samples. | to compare two selective agars, cellobiose-colistin (cc) agar and a modification of the vibrio vulnificus medium (vvmc agar), for the isolation of vibrio vulnificus from environmental samples. | 2001 | 11473597 |
| isolation and characterization of a vibrio vulnificus mutant deficient in both extracellular metalloprotease and cytolysin. | we isolated a vibrio vulnificus mutant that was deficient in both metalloprotease and cytolysin by allelic exchange. the virulence of this mutant in mice and its cytotoxicity for hep-2 cells were comparable to those of the wild-type strain, indicating that neither factor was essential for these properties. the cytolysin, but not the protease, seemed to be important for causing damage in the alimentary tract of the mice. | 2001 | 11500479 |
| heterogeneity of environmental, retail, and clinical isolates of vibrio vulnificus as determined by lipopolysaccharide-specific monoclonal antibodies. | the opportunistic pathogen vibrio vulnificus expresses lipopolysaccharide (lps) antigens on its outer membrane surface. a serological typing system was developed for these antigens, utilizing the discriminatory recognition of monoclonal antibodies (mab) by elisa. mab were used to recognize five unique types of lps-associated antigens for examination of clinical. environmental, and retail isolates of v. vulnificus. the overall serotype profile of the clinical isolates was significantly different ... | 2001 | 11510655 |
| [vibrio vulnificus. a marine bacterium with lethal potential]. | vibrio vulnificus is a halophilic gram-negative rod widespread in the aquatic environment and associated with primary septicemia and severe wound infections. the first swedish case was reported in 1994. ever since, sporadic cases have occurred in the south of sweden whenever the coastal water temperature has exceeded 20 degrees c. critical for a successful outcome in these infections has been early diagnosis with appropriate antibiotic and surgical treatment. a review of this subject was prompte ... | 2001 | 11526658 |
| the importance of serum creatine phosphokinase level in the early diagnosis, and as a prognostic factor, of vibrio vulnificus infection. | vibrio vulnificus infection causes rapidly progressive skin lesions and sepsis in compromised hosts with liver cirrhosis, and is often fatal. early diagnosis and rapid treatment are important. | 2001 | 11531792 |
| essential role for estrogen in protection against vibrio vulnificus-induced endotoxic shock. | little is known about the underlying mechanisms that result in a sexually dimorphic response to vibrio vulnificus endotoxic shock. v. vulnificus is a gram-negative bacterium, considered one of the most invasive and rapidly fatal human pathogens known. however, 85% of individuals that develop endotoxic shock from v. vulnificus are males. using the rat, we have developed a model for v. vulnificus endotoxic shock that mimics the sexually dimorphic response in humans. gonadectomy in females results ... | 2001 | 11553550 |
| cloning and characterization of a blue fluorescent protein from vibrio vulnificus. | the blue fluorescent protein (bfpvv) gene bfpvv from vibrio vulnificus ckm-1 was cloned and sequenced. the transformants exhibited blue fluorescence when irradiated by uv source. nucleotide sequence analysis predicted an orf of 717 bp encoding a 239-amino-acid polypeptide with a calculated molecular mass of 25.8 kda. the nucleotide sequence of the bfpvv gene and its deduced amino acid sequence showed significant homology to those of the short-chain dehydrogenase/reductase (sdr) family proteins f ... | 2001 | 11554735 |
| [vibrio vulnificus infection of the soft tissues]. | 2001 | 11555919 | |
| field testing of a vaccine against eel diseases caused by vibrio vulnificus. | the field results of a vaccination programme against vibrio vulnificus serovar e (biotype 2) in a spanish eel farm are reported. a total of 9.5 million glass eels were vaccinated from january 1998 to march 2000 by prolonged immersion followed by 2 subsequent reimmunisations after 12 to 14 and 24 to 28 d, respectively. the acquired protection and the immune response against serovar e were estimated over a period of 6 mo after vaccination. a similar vaccination schedule was conducted with elvers i ... | 2001 | 11558727 |
| transmission to eels, portals of entry, and putative reservoirs of vibrio vulnificus serovar e (biotype 2). | vibrio vulnificus serovar e (formerly biotype 2) is the etiologic agent that is responsible for the main infectious disease affecting farmed eels. although the pathogen can theoretically use water as a vehicle for disease transmission, it has not been isolated from tank water during epizootics to date. in this work, the mode of transmission of the disease to healthy eels, the portals of entry of the pathogen into fish, and their putative reservoirs have been investigated by means of laboratory a ... | 2001 | 11571177 |
| snakebite complicated with vibrio vulnificus infection. | snakebite may be complicated with bacteria infections, most of which are gram-negative bacillus. snakebite complicated with vibrio vulnificus infection has not been reported previously. vibrio vulnificus infection is characterized by a necrotizing wound infection similar to the local lesion of severe snakebite. in vibrio infections, aggressive debridement with the administration of strong antibiotics as early as possible is the only effective treatment. we report a 79-y-o man who suffered from s ... | 2001 | 11577933 |
| vibrio vulnificus cytolysin induces superoxide anion-initiated apoptotic signaling pathway in human ecv304 cells. | previous studies showed that exposure to vibrio vulnificus cytolysin (vvc) caused characteristic morphologic changes and dysfunction of vascular structures in lung. vvc showed cytotoxicity for mammalian cells in culture and acted as a vascular permeability factor. in this study, the underlying mechanisms of vvc-induced cytotoxicity was investigated on ecv304 cell, a human vascular endothelial cell line. when cells were exposed to 0.4 hemolytic units (hu) of vvc, consecutive apoptotic events were ... | 2001 | 11591724 |
| identification of a group 1-like capsular polysaccharide operon for vibrio vulnificus. | virulence of vibrio vulnificus correlates with changes in colony morphology that are indicative of a reversible phase variation for expression of capsular polysaccharide (cps). encapsulated variants are virulent with opaque colonies, whereas phase variants with reduced cps expression are attenuated and are translucent. using tnphoa mutagenesis, we identified a v. vulnificus cps locus, which included an upstream ops element, a wza gene (wza(vv)), and several open reading frames with homology to c ... | 2001 | 11598064 |
| the c-terminal domain promotes the hemorrhagic damage caused by vibrio vulnificus metalloprotease. | vibrio vulnificus, an opportunistic human pathogen, produces a 45-kda zinc metalloprotease (v. vulnificus protease; vvp) as an important virulence determinant. vvp injected intradermally into the dorsal skin causes the hemorrhagic damage through specific degradation of type iv collage in the vascular basement membrane. the n-terminal 35-kda polypeptide (vvp-n), the catalytic domain, also evoked the hemorrhagic skin reaction within minutes. however, the hemorrhagic activity of vvp-n was one-third ... | 2001 | 11600151 |
| vibrio vulnificus as a health hazard for shrimp consumers. | over the last 30 years, a number of vibrio species found in the aquatic environment have been indicated as cause of disease in human beings. vibrio vulnificus is an emergent pathogen, an invasive and lethal marine bacterium related to wound infection and held accountable for gastroenteritis and primary septicemia. it occurs quite frequently in marine organisms, mainly in mollusks. this study aimed at isolating and identifying strains of v. vulnificus based upon the analysis of twenty samples of ... | 2001 | 11696848 |
| a fatal case of vibrio vulnificus presenting as septic arthritis. | vibrio vulnificus is an invasive gram-negative bacillus that may cause necrotizing cellulitis, bacteremia, and/or sepsis. although v vulnificus infection is uncommon, it is frequently fatal and is usually attributed to ingestion of raw shellfish or traumatic exposure to a marine environment; patients are also often found to have a hepatic disorder (cirrhosis, alcohol abuse, or hemochromatosis) or an immunocompromised health status, and most commonly present with septicemia or a wound infection. ... | 2001 | 11718594 |
| cloning and characterization of a periplasmic nuclease of vibrio vulnificus and its role in preventing uptake of foreign dna. | we have cloned a nuclease gene, vvn, from vibrio vulnificus, an estuarine bacterium that causes wound infections and septicemia in humans and eels. the gene contained a 696-bp open reading frame encoding 232 amino acids (aa), including a signal sequence of 18 aa. the deduced amino acid sequence of the mature nuclease predicted a molecular mass of 25 kda, which was confirmed by vital stain, and a pi of 8.6. vvn was produced in the periplasm of either v. vulnificus or recombinant escherichia coli ... | 2001 | 11133431 |
| growth and recovery of selected gram-negative bacteria in reconditioned wastewater. | previous reports indicate that escherichia coli o157:h7, salmonella spp., and vibrio cholerae can grow in nutrient-limited, reconditioned wastewater over the temperature range of 4 to 46 degrees c when the biological oxygen demand of this water is <2, while its coliform growth response (cgr) is >2. in the current study, we investigated the growth response of vibrio parahaemolyticus, shigella spp., vibrio vulnificus, and pseudomonas aeruginosa in water samples with a cgr of >2 over the temperatur ... | 2001 | 11726156 |
| characterization of a vibrio vulnificus lysr homologue, hupr, which regulates expression of the haem uptake outer membrane protein, hupa. | in vibrio vulnificus, the ability to acquire iron from the host has been shown to correlate with virulence. here, we show that the dna upstream of hupa (haem uptake receptor) in v. vulnificus encodes a protein in the inverse orientation to hupa (named hupr). hupr shares homology with the lysr family of positive transcriptional activators. a hupa-lacz fusion contained on a plasmid was transformed into fur(-), fur(+)and hupr(-)strains of v. vulnificus. the beta-galactosidase assays and northern bl ... | 2001 | 11747377 |
| skin microflora and bacterial infections of the skin. | the skin is a milieu for controlled bacterial growth. skin supports the growth of commensal bacteria, which protect the host from pathogenic bacteria. environmental and local factors, host immunity, and organism adherence and virulence are intricately related to cutaneous infection. resident gram-positive bacteria include staphylococcus, micrococcus, and corynebacterium sp. staphylococcus aureus and streptococcus pyogenes are notoriously pathogenic in the skin. in order for bacteria to be pathog ... | 2001 | 11924823 |
| smcr-dependent regulation of adaptive phenotypes in vibrio vulnificus. | vibrio vulnificus contains homologues of the v. harveyi luxr and luxs genes. a null mutation in smcr (luxr) resulted in a defect in starvation survival, inhibition of starvation-induced maintenance of culturability that occurs when v. vulnificus is starved prior to low-temperature incubation, and increased expression of stationary-phase phenotypes. | 2001 | 11133972 |
| microbiological quality and safety of quahog clams, mercenaria mercenaria, during refrigeration and at elevated storage temperatures. | the effects of storage temperatures and times on the microbiological quality and safety of hard-shelled quahog clams (mercenaria mercenaria) were examined. samples were stored at four different incubation temperatures (3.3, 7.2, 10.0, and 12.8 degrees c) for a period of 3 weeks, following their harvest from summer growing waters (> or = 27 degrees c) and winter waters (< or = 4 degrees c). clams were analyzed for two naturally occurring pathogens, vibrio parahaemolyticus and vibrio vulnificus. d ... | 2001 | 11252477 |
| dna packaging and developmental intermediates of a broad host range vibrio vulnificus bacteriophage 71a-6. | the structural intermediates in the capsid assembly and dna packaging pathway of vibrio vulnificus bacteriophage 71a-6, a rod-shaped double-stranded dna podovirus, were isolated by ultracentrifugation and studied by electron microscopy, sds-page and pulsed-field gel electrophoretic analysis. bacteriophage 71a-6 synthesized rod-shaped capsids (mean length=200+/-8 nm; mean width=47+/-3 nm n=50) during its development. several host proteins that probably help in the assembly and maturation of the c ... | 2001 | 11292322 |
| identification and characterization of the soda genes encoding manganese superoxide dismutases in vibrio parahaemolyticus, vibrio mimicus, and vibrio vulnificus. | sequencing of fur titration assay-positive clones obtained from genomic dna libraries of vibrio parahaemolyticus, v. mimicus and v. vulnificus revealed open reading frames encoding proteins of 202, 205 and 202 amino acid residues, respectively. each open reading frame was preceded by a predicted fur box which overlaps a likely promoter with similarity to the -10 and -35 consensus sequence of escherichia coli. the deduced amino acid sequences shared considerable homology with bacterial mn-contain ... | 2001 | 11293479 |
| differential expression of vibrio vulnificus elastase gene in a growth phase-dependent manner by two different types of promoters. | elastase activity of vibrio vulnificus was highly dependent on growth phase, reached a maximum during the stationary phase, and was regulated at the level of transcription. the stationary phase production of elastase in crp or rpos mutants, which were constructed by allelic exchanges, decreased about 3- and 10-fold, respectively. however, the promoter activity of vvpe encoding elastase was unaffected by those mutations in the log phase when analyzed using a vvpe-lux fusion. a primer extension an ... | 2001 | 11297524 |
| effect of sub-mics of antibiotics on the hydrophobicity and production of acidic polysaccharide by vibrio vulnificus. | subinhibitory concentrations of antibiotics, which can occur in vivo, have been demonstrated to alter the production of bacterial virulence factors, including the capsule, or the interaction between microorganism and phagocyte by affecting surface hydrophobicity. | 2001 | 11306787 |
| regulation of metalloprotease gene expression in vibrio vulnificus by a vibrio harveyi luxr homologue. | expression of the vibrio vulnificus metalloprotease gene, vvp, was turned up rapidly when bacterial growth reached the late log phase. a similar pattern of expression has been found in the metalloprotease gene of vibrio cholerae, and this has been shown to be regulated by a vibrio harveyi luxr-like transcriptional activator. to find out whether a luxr homologue exists in v. vulnificus, a gene library of this organism was screened by colony hybridization using a probe derived from a sequence that ... | 2001 | 11157950 |
| [identification of vibrio vulnificus from patients with otitis media and septicemia by pcr method]. | sucrose-negative colonies were isolated on tcbs agar plate from otorrhea specimens from otitis media patient in the adult diseases examination center, hirosaki medical association. the isolate was identified as vibrio vulnificus by nested pcr method which amplify v. vulnificus-specific sequences directed against 23s rrna genes. the pcr method was applied to identify 6 strains originally isolated from septicemia patients in kurashiki central hospital and formerly identified as v. vulnificus by ph ... | 2001 | 11357321 |
| effects of bacteria on the growth of an amoeba infecting the gills of turbot. | we analysed the influence of various bacteria on the in vitro growth of trophozoites of a platyamoeba strain isolated from diseased gill tissues of cultured turbot. little or no growth was shown by amoebae cultured in the presence of (1) the turbot-pathogenic bacteria vibrio anguillarum, aeromonas salmonicida or streptococcus sp., (2) pasteurella piscicida or vibrio vulnificus (pathogenic for some fishes but not turbot), or (3) the non-pathogenic 'environmental' bacteria vibrio campbelli, vibrio ... | 2001 | 11411647 |
| levels of vibrio vulnificus and organoleptic quality of raw shellstock oysters (crassostrea virginica) maintained at different storage temperatures. | temperature abuse during raw oyster harvesting and storage may allow for the multiplication of natural spoilage flora as well as microbial pathogens, thus posing a potential health threat to susceptible consumers and compromising product quality. the objective of this study was to provide a scientific basis for determining whether different refrigeration and abuse temperatures for raw oysters would result in a spoiled product before it became unsafe. raw shellstock oysters (crassostrea virginica ... | 2001 | 11726149 |
| ventricular arrhythmias: some pathophysiological aspects and therapeutic problems. | 2001 | 95451 | |
| origin of maternal serum alpha-fetoprotein. | 2001 | 93217 | |
| an improved immunoelectrophoretic methodology for the evaluation of protein caloric malnutrition. | an improved technique of two dimensional immunoelectrophoresis of serum proteins on gelatinized cellulose acetate for routine evaluation of protein nutritional state is described. the method, which allows the estimation of more than 30 protein fractions, proves extremely useful in monitoring the effectiveness of total parenteral nutrition and protein metabolic conditions. sera samples from healthy subjects and cachectical neoplastic patients have been examined and preliminary results are reporte ... | 2001 | 92995 |
| [evolution of the free thyroxine index during pregnancy]. | 2001 | 95054 | |
| severe handicap in the newborn: treatment, non-treatment, or assisted death. | 2001 | 92640 | |
| [evaluation of the beta-galactosidase test for the identification of enterobacteria]. | 2001 | 92602 | |
| [long-term results of synchronized radiotherapy in inoperable orofacial squamous cell carcinoma]. | 2001 | 95490 | |
| reconstructive operations on tendons and a tendo-ligamentous apparatus. | 2001 | 92871 | |
| random amplified polymorphic dna analysis to differentiate strains of vibrio vulnificus isolated from cockles and shrimps. | a random amplified polymorphic dna (rapd) fingerprinting method has been developed to differentiate vibrio vulnificus strains isolated. twenty-nine strains isolated from cockles and twenty-one strains isolated from shrimps were analyzed. a total of 10 primers were screened with vibrio vulnificus strains to identify those capable of generating dna polymorphisms and two primers were selected. primer gen 1-50-01 and gen 1-50-08 produced polymorphisms in most strains tested, with the band sizes rang ... | 2000 | 22844214 |
| acyclovir for treatment of mucocutaneous herpes infection in a child with leukaemia. | 2000 | 91931 | |
| [arrhythmias in direct fetal electrocardiography]. | 2000 | 92107 | |
| genital herpes zoster: a case resembling primary herpes progenitalis with distal urethral involvement. | 2000 | 101907 | |
| oral methionine loading as a cause of acute serum folate deficiency: its relevance to parenteral nutrition. | oral methionine supplements (8.0 g daily for 4 days) were given to five normal volunteers who continued to eat their usual diet. this treatment resulted in a significant fall in serum folate concentration. three days after the end of treatment concentrations had not completely returned to control values. the fall in concentration was prevented by giving oral folic acid supplements. it is suggested that folic acid supplements should be given to patients who are receiving intravenous infusions of ... | 2000 | 97644 |
| [treatment of diabetic ketoacidosis and hyperosmolality with low dose insulin infusion (author's transl)]. | fifteen patients were treated with low-dose (5 u/hour) insulin infusion, including 10 cases of ketoacidosis, 3 cases of hyperglycemia without acidosis in severely affected diabetics, and 2 cases with hyperosmolality. the treatment was successful in all cases. insulin was infused at a constant rate, during 12 hours as a mean value. blood glucose fell regularly and no hypoglycemia occured. serum potassium varied within narrow limits, and no accident related to hypokalemia was observed. the correct ... | 2000 | 97626 |
| reversal of florid diabetic retinopathy. | 2000 | 91797 | |
| [a delicate intervention: tonsillectomy in adults]. | 2000 | 92060 | |
| can the family physician avoid conflict of interest in the gatekeeper role? an affirmative view. | 2000 | 2723596 | |
| [legislative regulation of mental health care]. | 2000 | 3406216 | |
| [fulminant sepsis caused by vibrio vulnificus. a case series]. | vibrio vulnificus is a marine bacteria associated with the ingestion of raw shellfish or contact with seawater. it can produce wound infection, diarrhea and sepsis. the main risk factor for infection is the presence of chronic liver disease. prior studies have shown mortality from 40% to 63%. | 2000 | 11256106 |
| effectiveness of different vaccine formulations against vibriosis caused by vibrio vulnificus serovar e (biotype 2) in european eels anguilla anguilla. | vibriosis due to vibrio vulnificus serovar e (biotype 2) is one of the main causes of mortality in european eels cultured in europe. the main objective of this study was to develop a vaccine and a vaccination procedure against this pathogen. with this aim, we tested several vaccine formulations (inactivated whole-cells with and without toxoids--inactivated extracellular products--from capsulated and uncapsulated strains, attenuated live vaccines and purified lipopolysaccharide [lps]) on eels mai ... | 2000 | 11145457 |
| detection and molecular characterization of vibrio vulnificus from coastal waters of malaysia. | a total of 57 vibrio vulnificus isolates from coastal water were characterized for their antimicrobial resistance, plasmid profiles and were typed by the pcr-based techniques: a random amplification of polymorphic dna (rapd) method and the enterobacterial repetitive intergenic consensus sequence (eric) method. all isolates were susceptible to chloramphenicol, nalidixic acid, tetracycline and trimethoprim-sulfamethoxazole. fifty-one isolates were resistant to one or more of the other antibiotics ... | 2000 | 11414409 |
| [effects of vibrio vulnificus metalloprotease on the capillaries: pathological actions and inactivation by alpha-macroglobulin]. | vibrio vulnificus is an opportunistic human pathogen causing wound infection and septicemia, characterized by hemorrhagic and edematous damage to the skin of limbs. when injected into the dorsal skin, an extracellular metalloprotease from this vibrio (v. vulnificus protease: vvp) enhanced the vascular permeability through activation of the hageman factor-plasma kallikrein-kinin cascade and/or stimulation of exocytotic histamine release. additionally, vvp caused the hemorrhagic skin lesion throug ... | 2000 | 11190200 |
| a highly homologous 68 kbp plasmid found in vibrio vulnificus strains virulent for eels. | vibrio vulnificus serovar e (biotype 2) strains are virulent for eels and have also been reported to cause illness in humans. studies on the plasmid content of serovar e strains revealed the existence of a plasmid of approximately 70 kbp present in most of these strains. in this study we characterized the 70 kbp plasmids of seven biotype 2 strains isolated from seawater, diseased eels and wound infections in humans. we determined the exact size of the high molecular weight plasmids to be 68 kbp. ... | 2000 | 11199498 |
| [analysis of the plasmid profile of various salmonella serotypes]. | every year foodborne infections cause millions of illnesses but many of them go undiagnosed and unreported. the epidemiology of these illnesses is changing, new pathogens have emerged (escherichia coli o157:h7, cyclospora cayetanensis, vibrio vulnificus). salmonella spp. is the most common bacterial cause of acute enterocolitis with us. all over the world, as well as in our country the most often isolated serotype is salmonella enteritidis. a great problem in many countries is the multiresistant ... | 2000 | 11320741 |
| mechanism of high susceptibility of iron-overloaded mouse to vibrio vulnificus infection. | vibrio vulnificus produces fulminant septicemia in humans with underlying conditions, particularly those with diseases that elevate the iron level. the effect of a high iron level on the virulence of v. vulnificus was therefore investigated in mice treated with iron dextran. the mice loaded with iron became highly susceptible to v. vulnificus infection, the ld50 (50% lethal dose) decreased five logs when infected per peritoneum. however, when infected via the oral route, the ld50 was affected li ... | 2000 | 11145266 |
| cloning and characterization of vuua, a gene encoding the vibrio vulnificus ferric vulnibactin receptor. | the ability of vibrio vulnificus to acquire iron from the host has been shown to correlate with virulence. many iron transport genes are regulated by iron, and in v. vulnificus, transcriptional regulation by iron depends on the fur gene. the n-terminal amino acid sequence of a 72-kda iron-regulated outer membrane protein purified from a v. vulnificus fur mutant had 53% homology with the first 15 amino acids of the mature protein of the vibrio cholerae vibriobactin receptor, viua. in this report, ... | 2000 | 10639413 |
| vibrio vulnificus sepsis. | 2000 | 11878490 | |
| an indirect immunofluorescent antibody technique for detection and enumeration of vibrio vulnificus serovar e (biotype 2): development and applications. | the applications of an indirect fluorescent antibody technique (ifat), developed to detect and enumerate the pathogenic bacterium vibrio vulnificus serovar e from water and clinical samples, are described. this technique proved accurate for detecting v. vulnificus, even under starvation conditions and in the non-culturable state, and could differentiate this species from other bacteria which share the same habitats. the ifat was successfully used to diagnose vibriosis from naturally- and artific ... | 2000 | 11054163 |
| a case of fatal food-borne septicemia: can family physicians provide prevention? | vibrio vulnificus, a common bacteria found in undercooked seafood and seawater, is the leading cause of food-borne death in florida. fatal cases of v vulnificus infection have also been reported in most states. | 2000 | 10826868 |
| epidemiology and pathogenesis of vibrio vulnificus. | vibrio vulnificus is capable of causing severe and often fatal infections in susceptible individuals. it causes two distinct disease syndromes, a primary septicemia and necrotizing wound infections. this review discusses the interaction of environmental conditions, host factors, and bacterial virulence determinants that contribute to the epidemiology and pathogenesis of v. vulnificus. | 2000 | 10742690 |
| vibrio vulnificus corneal ulcer: rapid resolution of a virulent pathogen. | to describe a corneal ulcer due to vibrio vulnificus that resolved rapidly with antibiotic therapy alone. | 2000 | 10632019 |
| vibrio vulnificus: a physiological and genetic approach to the viable but nonculturable response. | in this review, we focus on studies of the viable but nonculturable response (vbnc) of vibrio vulnificus, a significant and aggressive human pathogen, as a model system for the general understanding of the vbnc response. this response is characterized physiologically as the inability to culture an organism on media that normally supports its growth, and yet those cells retain indicators of metabolic activity. implicit in this definition is that it may be possible to return or resuscitate vbnc ce ... | 2000 | 11810549 |
| microbial pathogens with impaired ability to acquire host iron. | successful microbial pathogens must be adept in obtaining growth-essential iron from healthy hosts. some potential pathogens, however, are sufficiently impaired in iron acquisition ability so as to be dangerous mainly in hosts with such iron loading conditions as alcoholism, asplenia, hemochromatosis, beta-thalassemia major, or tobacco smoking. the association of six impaired pathogens (capnocytophaga canimorsis, yersinia enterocolitica and y. pseudotuberculosis, vibrio vulnificus, tropheryma wh ... | 2000 | 10831229 |
| renal failure in vibrio vulnificus infection. | vibrio vulnificus infection with septicemia is a life threatening disease in the immunocompromised hosts. renal involvement has not been documented. we reported herein 8 patients with v. vulnificus septicemia. all were immunocompromised hosts. four patients had cirrhosis of the liver, 3 were heavy alcohol drinkers and one had systemic lupus erythematosis. presenting symptomatology included fever, chills, leg pain and skin rash. renal failure was observed in 6 patients. four patients died shortly ... | 2000 | 10843244 |
| vibrio vulnificus has the transmembrane transcription activator toxrs stimulating the expression of the hemolysin gene vvha. | in an attempt to dissect the virulence regulatory mechanism in vibrio vulnificus, we tried to identify the v. cholerae transmembrane virulence regulator toxrs (toxrs(vc)) homologs in v. vulnificus. by comparing the sequences of toxrs of v. cholerae and v. parahaemolyticus (toxrs(vp)), we designed a degenerate primer set targeting well-conserved sequences. using the pcr product as an authentic probe for southern blot hybridization, a 1.6-kb bglii-hindiii fragment and a 1.2-kb hindiii fragment con ... | 2000 | 10852871 |
| evaluation of a simplified semi-quantitative protocol for the estimation of vibrio vulnificus in bathing water using cellobiose-colistin agar: a collaborative study with 13 municipal food controlling units in denmark. | a simplified semi-quantitative method using pre-enrichment in alkaline peptone water supplemented with polymyxin b and plating onto cellobiose-colistin (cc) agar for the estimation of vibrio vulnificus in bathing water was evaluated. this protocol was tested in a collaborative study with 13 food controlling laboratories in denmark during the 1999 bathing season in periods when water temperatures exceeded 20 degrees c. the average percentage of yellow colonies larger than 1 mm in diameter on cc a ... | 2000 | 10856777 |
| protective effect of c-reactive protein against the lethality induced by vibrio vulnificus lipopolysaccharide. | vibrio vulnificus infection has attracted special interest because of its high mortality. a strong clinical association exists between hepatic dysfunction and increased morbidity and mortality from v. vulnificus infection. in this study, the effect of c-reactive protein (crp), a typical hepatogenic acute phase protein, on the lethality induced by v. vulnificus lipopolysaccharide (lps) was investigated in galactosamine-sensitized mice. the pretreatment of crp, in a dose of at least 2 mg/kg, 2 hr ... | 2000 | 10888350 |