Publications

[risk factors for condylomata. a case control study].	the present study involves 214 patients whose cervical smear showed signs of human papilloma virus infection (hpv), matched with 1042 controls. we demonstrate increasing odds ratio with the total number of sexual partners since first sexual intercourse (p less than 0.0001). moreover the cases have more often changed partners in the year preceding the diagnosis (odds ratio 2.6 p less than 0.0001). their male partners more often have occupations that expose them to "extra-conjugal adventures" (p = ...	1989	2600372
cockroaches (periplaneta americana) as carriers of agents of bacterial diarrhoea in accra, ghana.	bodies and intestinal contents of 208 cockroaches (periplaneta americana), collected from kitchens in accra and some surrounding villages were cultured for enteric bacterial pathogens. six of them harboured three different serogroups of salmonella, one had shigella dysenteriae, 64 had coliforms, 13 had proteus species, two had pseudomonas species and the rest (122) carried none of the bacterial species mentioned above. the presence of salmonella species shigella dysenteriae and coliforms in thes ...	1989	2611875
histotypic differentiation of neonatal mouse retina in organ culture.	retinae from neonatal mice were explanted in toto, with or without the retinal pigment epithelium (rpe) and adjoining mesenchymal cells, and maintained in organ culture for up to 3 weeks. the explants remained flat, rosette formation was minimal and histogenetic changes followed in the normal sequence. after 11, 14 and 21 days in vitro the three cellular layers--the outer nuclear layer including well differentiated rod and cone perikarya, the inner nuclear layer and the ganglion cell layer--with ...	1989	2612197
histotypic differentiation of neonatal mouse retina in organ culture.	retinae from neonatal mice were explanted in toto, with or without the retinal pigment epithelium (rpe) and adjoining mesenchymal cells, and maintained in organ culture for up to 3 weeks. the explants remained flat, rosette formation was minimal and histogenetic changes followed in the normal sequence. after 11, 14 and 21 days in vitro the three cellular layers--the outer nuclear layer including well differentiated rod and cone perikarya, the inner nuclear layer and the ganglion cell layer--with ...	1989	2612197
indomethacin blocks the anorexic action of interleukin-1.	it has been reported recently that the central nervous system actions of interleukin-1 are mediated by the prostaglandin system in the brain. the present study was therefore performed in order to examine the hypothesis that indomethacin, an inhibitor of prostaglandin biosynthesis, might alleviate the interleukin-1-induced suppression of food intake in rats. the i.p. injection of interleukin-1 (2 micrograms/rat) resulted in a significant decrease in food intake. the pre-injection of indomethacin ...	1989	2620696
indomethacin blocks the anorexic action of interleukin-1.	it has been reported recently that the central nervous system actions of interleukin-1 are mediated by the prostaglandin system in the brain. the present study was therefore performed in order to examine the hypothesis that indomethacin, an inhibitor of prostaglandin biosynthesis, might alleviate the interleukin-1-induced suppression of food intake in rats. the i.p. injection of interleukin-1 (2 micrograms/rat) resulted in a significant decrease in food intake. the pre-injection of indomethacin ...	1989	2620696
isolation of inhibitors of adenylate cyclase from dan-shen, the root of salvia miltiorrhiza.	the effect of dan-shen extract, the root of salvia miltiorrhiza, on adenylate cyclase was investigated in both rat brain and rat erythrocytes. the etoac fraction of the meoh extract was proved to have significant inhibitory activity. potent inhibitory principles in the etoac fraction were isolated and identified as 4 polyphenolic acids, rosemarinic acid, lithospermic acid, and their methyl ester derivatives.	1989	2630095
[detection of streptococci from various serological groups in animals].	during the period from 1985 to 1988 we determined 228 strains of streptococci isolated from samples of various sorts of biomaterials, mainly animals. from this set of streptococci we classified 207 strains into serological groups according to the lancefield classification and about 30% strains into species by means of serological and biochemical methods. most of the strains were allocated to group c (37.28%) and group q (17.39%). 89 streptococci strains originated from pigs, 40 strains from hors ...	1989	2631378
[serological diagnosis of campylobacter jejuni infections].	antibody level to campylobacter in 28 sera of patients of whom campylobacter infection was confirmed by germ isolation from feces was tested. the investigation was performed using passive haemagglutination technique and as antigens heated and acid glycine extraction prepared from homologous and reference strains. for the method used the heated antigen proved to be superior. out of 28 tested patients of whom 92.8% were children, 8 sera were positive, 9 doubtful and 9, derived mainly from neonates ...	1989	2640683
detection of enterotoxigenic escherichia coli after polymerase chain reaction amplification with a thermostable dna polymerase.	the direct identification of enterotoxigenic escherichia coli from clinical specimens was examined by using the polymerase chain reaction (pcr) for amplifying the heat-labile toxin (lt) gene. two synthetic primers, each of which was 20 bases in length, were used with the thermostable dna polymerase from thermus aquaticus to amplify the lt gene. the amplified pcr products were detected by either gel electrophoresis or hybridization to a 24-base synthetic oligonucleotide probe conjugated to alkali ...	1989	2644292
introduction and spread of multi-resistant shigella dysenteriae i in thailand.	outbreaks of shigella dysenteriae i occurred in northeastern thailand in the fall of 1986 and again in the spring and fall of 1987 for the first time in over 20 years. the epidemic strain of s. dysenteriae i was resistant to tetracycline, streptomycin, chloramphenicol, and trimethoprim-sulfamethoxazole, but susceptible to ampicillin. trimethoprim resistance was chromosomally encoded by type i dihydrofolate reductase. in ubon province, where 10,000 cases of dysentery were reported, there were 3-5 ...	1989	2644859
plasmid dna analysis by agarose gel electrophoresis on an epidemic-associated strain of trimethoprim resistant shiga's bacillus from gimira wereda (keffa administrative region, southwest ethiopia).	a combination of genetic mating experiments and plasmid dna analysis by gel electrophoresis was carried out on an epidemic-associated shiga's bacillus with resistance type acssuttp, isolated in 1983 from gimira wereda, keffa administrative region, south west ethiopia. the donor strain contained 9 plasmid species. their sizes in megadaltons (mdal) were: 120, 40, 35, 12.5, 6.0, 5.1, 4.3, 2.5 and 1.7. analysis of escherichia coli k12 transconjugants revealed that the 40, 35, and 4.3 mdal plasmids c ...	1989	2646117
renin: endocrine, paracrine, or part-paracrine control of blood pressure?		1989	2650710
pathogenesis of shigella diarrhea. xiv. analysis of shiga toxin receptors on cloned hela cells.	binding kinetics of shiga toxin to hela ccl-2 cells and to cell lines cloned by limiting dilutions were determined. lines with a wide range of sensitivity to shiga toxin were obtained. binding data, analyzed by a computer-based scatchard model program, revealed two classes of binding sites, one of low affinity and high capacity and one of high affinity and low capacity. the number of high affinity, but not low affinity, sites present on the clones correlated with their sensitivity to toxin. tuni ...	1989	2651533
interaction of shigella toxin with globotriaosyl ceramide receptor-containing membranes: a fluorescence study.	the interaction of the b-subunit of shigella toxin with a globotriaosyl ceramide receptor incorporated into phosphatidylcholine vesicles was studied by fluorescence spectroscopy. from the position of the maximum in the emission spectrum and the accessibility to acrylamide quenching, it is concluded that a single tryptophan of a free b-chain is located in a highly polar environment, most likely on the surface of the folded polypeptide chain. binding of b-subunits to the membrane-associated globot ...	1989	2653314
malignant hyperpyrexia.		1989	2653537
unusual nucleotide arrangement with repeated sequences in the escherichia coli k-12 chromosome.	between 59 and 60 min on the escherichia coli genetic map, there is a highly conserved sequence of 29 base pairs, containing an inverted repeat of seven base pairs that appears 14 times, 32 or 33 base pairs apart, downstream of the iap gene coding region. about 24 kilobase pairs downstream of the 14 repeats, a similar 29-base-pair sequence with a spacing of 32 base pairs appears seven times. nucleotide sequences hybridizing with the 29-base-pair fragment were also detected in shigella dysenteria ...	1989	2656660
inoculum size in shigellosis and implications for expected mode of transmission.		1989	2656880
[survey on overseas traveller's diarrhea at narita airport quarantine station--the species of bacteria isolated and the suspected countries for infection].		1989	2657132
[clinical features of overseas travellers' infectious enteritis admitted in the hospitals for infectious diseases].		1989	2657133
secretory immunoglobulin a response to shiga toxin in rabbits: kinetics of the initial mucosal immune response and inhibition of toxicity in vitro and in vivo.	although the role of shiga toxin in dysentery is unknown, the toxin is cytotoxic to hela cells, causes fluid secretion in rabbit intestine, and is lethal to rabbits and mice when injected parenterally. in the present study, rabbits received three weekly doses of shiga toxin directly into chronically isolated ileal loops. within a week, secretions from these loops contained immunoglobulin a (iga) anti-shiga toxin. the titer of iga anti-shiga toxin increased after weekly doses 2 and 3. little igg ...	1989	2659525
epidemic shigellosis due to shigella dysenteriae type 1 in south asia.		1989	2661420
quantitation of the rabbit intestinal glycolipid receptor for shiga toxin. further evidence for the developmental regulation of globotriaosylceramide in microvillus membranes.	shiga toxin, produced by shigella dysenteriae 1, causes enterotoxic, cytotoxic, and neurotoxic effects, which may be mediated by a glycolipid receptor, globotriaosylceramide, gb3. to study the relationship of this receptor and toxin effects, globotriaosylceramide was quantitated and further characterized in rabbit small intestinal microvillus membranes at various ages. glycolipids were extracted from rabbit microvillus membranes, purified on unisil columns, and quantitated by high-performance li ...	1989	2663611
congo red-mediated regulation of levels of shigella flexneri 2a membrane proteins.	the ability of shigella spp. to bind congo red from agar medium is generally correlated with their virulence properties. we used a metabolically active culture of shigella flexneri 2a to determine the effect of congo red on its membrane protein profiles. virulent s. flexneri grown in the presence of congo red at 37 degrees c showed increased levels of three proteins with mrs of 43,000, 58,000, and 63,000 (43k, 58k, and 63k proteins) in the sarkosyl-soluble membrane fractions. the observed phenom ...	1989	2663721
rapid method to detect shiga toxin and shiga-like toxin i based on binding to globotriosyl ceramide (gb3), their natural receptor.	shiga toxin and the closely related shiga-like toxins produced by escherichia coli represent a group of very similar cytotoxins that may play an important role in diarrheal disease and hemolytic uremic syndrome. these toxins have the same biologic activities and according to recent studies also share the same binding receptor, globotriosyl ceramide (gb3). they are currently detected, on the basis of their ability to damage several cell lines, by using expensive and tedious assays that require fa ...	1989	2666433
use of monoclonal antibodies to type shigella flexneri in bangladesh.	a panel of 10 mouse and rat monoclonal antibodies specific for different type- and group-specific o-antigenic determinants of shigella flexneri lipopolysaccharide was used to serotype 240 isolates of s. flexneri from bangladesh. three immunoglobulin m antibodies were used in a direct slide agglutination test; seven immunoglobulin g antibodies were absorbed to staphylococcus aureus and used in a coagglutination assay. all but 13 of the isolates could be serotyped by using the monclonal antibodies ...	1989	2666435
cryptococcus neoformans in the seminal fluid of an aids patient. a contribution to the clinical course of cryptococcosis.	in a 33-year-old hiv-positive homosexual male suffering from unexplained headache, cryptococcosis was diagnosed in a progressive secondary stage. after treatment with the standard combination therapy of amphotericin b + flucytosine for 34 d, the patient was clinically symptom-free and discharged, upon his own request, from the hospital. he remained under ambulatory mycological control. after an interval of 65 d during which the urine had been free from cryptococcus neoformans (cr.n.), the fungus ...	1989	2666852
purification, crystallization, and preliminary x-ray diffraction studies of the flavoenzyme mercuric ion reductase from bacillus sp. strain rc607.	the flavoenzyme mercuric ion reductase from bacillus sp. strain rc607 was purified by dye-ligand affinity chromatography. the protein was crystallized from solutions of high ionic strength, and one of the two crystal forms obtained has proven suitable for x-ray diffraction studies. preliminary analysis showed that these crystals belong to the tetragonal space group 1422. the unit cell dimensions are a = b = 180.7 a; c = 127.9 a. the diffraction pattern extends to better than 3 a resolution. crys ...	1989	2668287
hemolytic uremic syndrome and diarrhea in argentine children: the role of shiga-like toxins.	shiga-like toxin-producing escherichia coli have been associated with hemorrhagic colitis and the hemolytic uremic syndrome (hus). because argentina has the highest reported frequency of hus in the world, argentine children were prospectively studied during the hus seasons for evidence of shiga-like toxin-related diseases. on the basis of serology, fecal cytotoxin neutralization, stool cultures, and dna hybridization of colony lysates, most children with hus had evidence of infection with shiga- ...	1989	2668430
[bacillary dysentery--1987].		1989	2669032
antibiotics in the management of shigellosis in children: what role for the quinolones?	in the last 20 years, epidemic bacillary dysentery due to shigella dysenteriae type 1 has become an important cause of serious morbidity and death in children less than 5 years of age in a number of developing countries; in many instances the infecting strains have been resistant to many or all of the usually recommended antibiotics. moreover, an increasing proportion of shigella strains of other serotypes isolated in all parts of the world are now resistant to the most commonly used antibiotics ...	1989	2672246
mechanisms of tumor induction by human retroviruses.	oncogenesis can be induced by chronic and acute rna tumor viruses through a promoter/enhancer insertion mechanism and through a transforming protein coded by a viral oncogene (v-onc), respectively. a hypothetical trans-activating mechanism is invoked by retroviruses with trans-activating regulatory proteins. so far, in humans only htlv-i has been firmly linked to a malignancy. the same virus is also implicated in slowly progressing neurological disorders. except for transformation by viral oncog ...	1989	2673254
clinical & bacteriological profiles of shigellosis in calcutta before & after an epidemic (1984-87).	patients below 5 yr of age, hospitalised for shigellosis over a period of four years (1984-87), were studied. during the epidemic of bacillary dysentery (1984) isolation of different shigella spp. as well as shigella dysenteriae type 1 was high. decreased isolation of sh. dysenteriae type 1 and increased isolation of sh. flexneri was observed during post-epidemic years (1985-87). isolation of different shigella spp. was always above 25 per cent from patients with dysentery and greater than 7 per ...	1989	2674001
lymphocyte homing.	the phenomenon of "homing" is discussed with respect to patterns of lymphocyte circulation and the molecules on the surface of both endothelium and lymphocytes that mediate this process. in addition, the data are analysed in the context of a model for lymphocyte homing.	1989	2675225
isolation and characterization of functional shiga toxin subunits and renatured holotoxin.	shiga toxin is a protein toxin produced by shigella dysenteriae type i strains. in this report we present a procedure for the separation of functionally intact toxin a and b chains and for their reconstitution to form biologically active molecules. in agreement with the findings of others, the isolated a chain was shown to be a potent in vitro inhibitor of eukaryotic protein synthesis. the isolated b chain bound to hela cells and competitively inhibited the binding and cytotoxic activity of holo ...	1989	2677606
normal catch-up growth in rats severely food-restricted prior to lesions of the dorsomedial hypothalamic nucleus: the first 48 hours.	following a brief period of ad lib (al) feeding, 45-day-old male sprague-dawley rats were either fed al or food-restricted (rest) for 21 days to 50% of the intake of the al rats. at this time, some al and some rest rats received electrolytic lesions in the dorsomedial hypothalamic nuclei (dmnl), whereas other al and rest rats were sham-operated (con). following this, all rats were refed (ref) al and killed two days later. at this time, dmnl-rest + ref and dmnl-al weighed as much as con-rest + re ...	1989	2678164
thymotaxin, a chemotactic protein, is identical to beta 2-microglobulin.	thymotaxin, an 11-kilodalton protein chemotactic for rat bone marrow hematopoietic precursors, was purified from media conditioned by a rat thymic epithelial cell line. the nh2-terminal sequence of thymotaxin was identical to that of rat beta 2-microglobulin (beta 2m). antibodies to beta 2m removed thymotaxin activity from the fraction containing the 11-kilodalton protein. chemotactic activity was observed with rat plasma beta 2m, human beta 2m, and mouse recombinant beta 2m, further supporting ...	1989	2683083
[cellular lectin binding sites in the human retinal pigment epithelium and photoreceptors--method of lectin histochemistry and results with peroxidase marked lectins in paraffin sections].	lectins are sugar-binding proteins or glycoproteins which bind to specifically complementary sugar residues found in cell membranes and the cytoplasm. lectin-binding sites are determined by histochemical methods. cellular lectin-binding sites change with morphological and functional alterations of the cell, and the determination of lectin receptors can be used to identify specific cell types in histological sections. lectin-binding sites of the human retinal pigment epithelium (rpe) and photorec ...	1989	2684825
monoclonal antibodies against the surface antigens of shigella flexneri serotype 1b and shigella dysenteriae serotype 1.	monoclonal antibodies against the surface antigens of shigella flexneri 1b and s. dysenteriae 1 were prepared. the specificities of the antibodies were evaluated by enzyme-linked immunosorbent assay (elisa), and quantitative agglutination using microtiter plate. monoclonal antibodies against s. flexneri 1b, designated sf2b2 and sf2g4, belonged to igg2a and igg1 subclass, respectively. the former was specific for s. flexneri 1b, whereas the latter was reactive not only to s. flexneri 1b, but also ...	1989	2687648
assay for sialidase using erythrocytes and peroxidase-labeled peanut lectin.	a rapid and sensitive sialidase assay method based on peroxidase-labeled peanut lectin (pna) binding to desialylated erythrocyte is described. formalinized sheep erythrocytes were used both as a stable substrate for sialidase and as a target for the lectin. in the case of sialidases from vibrio cholerae and arthrobacter ureafaciens, a linear relationship was observed between the amount of peroxidase-labeled pna bound to erythrocytes and the enzyme amount. binding of the lectin to sialidase-treat ...	1989	2688936
purification of shiga toxin by alpha-d-galactose-(1----4)-beta-d-galactose-(1----4)-beta-d-glucose-(1- ---) receptor ligand-based chromatography.	a simple and rapid method for shiga toxin purification based on specific binding to the gal alpha 1----4gal beta 1----4glc globotrioside trisaccharide covalently linked to polyacryl/polyvinyl (fractogel) has been developed. a cell-free sonicate-filtrate of shigella dysenteriae type 1, strain 114sd was passed over the globotrioside-fractogel column, and bound toxin eluted with 6 m guanidine-hcl. a yield of 36 mg pure toxin/1 sonicate-filtrate was obtained, i.e. a one step 1224-fold purification. ...	1989	2689221
nutritional anestrus in beef cows: concentrations of glucose and nonesterified fatty acids in plasma and insulin in serum.	twenty-two nonlactating hereford cows exhibiting normal estrous cycles were fed either maintenance (m) or restricted (r) diets until most of the r cows became anestrus; r cows then were fed 160% of the m diet until estrous cycles resumed. concentrations of progesterone, glucose, insulin and nonesterified fatty acids (nefa) were determined in weekly blood samples. blood also was collected frequently, before and after i.v. infusion of 300 ml of a 40% glucose solution, to evaluate responses in bloo ...	1989	2689418
production of vero cytotoxin by escherichia coli and shiga toxin by shigella dysenteriae 1 as related to the growth medium and availability of iron.	six strains of escherichia coli producing vero cytotoxin (vtec) and six strains of shigella dysenteriae 1 were examined for the production of extra- and intracellular vero cytotoxin (vt) and shiga toxin respectively, in relation to the growth medium and availability of iron. vtec secreted less extracellular vt1 or vt2 when grown in trypticase soy broth (tsb) containing the iron chelator desferal, as compared to bacteria cultured in iron replete tsb. growth in tsb containing desferal resulted in ...	1989	2692581
falciparum malaria--present day problems. an experience with 425 cases.	clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ...	1989	2693436
[shigellosis in the ussr: the etiological role of shigella dysenteriae].	the etiological role of s. dysenteriae in shigella infections at different territories of the ussr in 1983-1987 is analyzed. the study shows that s. dysenteriae pertain to territories with unfavorable water supply of the population. at some of these territories, in particular the uzbek ssr, grigor'ev-shiga dysentery seems to gain ground and the restoration of the area of its spread occurs. in recent years the penetration of grigor'ev-shiga dysentery from abroad became more frequent in the presen ...	1989	2694693
[drug resistance of the causative agent of grigo'rev-shiga dysentery (shigella dysenteriae 1)isolated in the ussr].	forty strains of s. dysenteriae 1 isolated in the ussr within 1986-1988 were tested for their resistance to 11 antibacterial drugs. it was shown that 92.5-97.5 per cent of the isolates were resistant to chloramphenicol (cm) and tetracycline (tc), 22.5 per cent to streptomycin (sm), 17.5 per cent to nalidixic acid (nal) and 10 per cent to ampicillin (ap) and polymyxin (pm). resistance to cm tc (51.4 per cent) and cm tc nal (13.5 per cent) represented the predominating phenotype. 35 per cent of th ...	1989	2694989
characterization and autoprocessing of precursor and mature forms of human immunodeficiency virus type 1 (hiv 1) protease purified from escherichia coli.	a recombinant plasmid encompassing the human immunodeficiency virus type 1 (hiv 1) protease coding sequence and flanking regions (ala-13 to gly-185 of the pol open reading frame) has been expressed in two distinct strains of escherichia coli, ar58 and ar68. in the first strain, ar58, the primary translation product, a 25 kilodalton (kda) precursor protein, is short-lived and rapidly processes itself to the 11 kda mature protease in vivo. in the second strain, ar68, the 25 kda species is only par ...	1989	2695927
[the etiological structure of shigellosis in the ussr in recent years].	the characterization of etiological structure of shigella infection in the whole of the ussr, in individual union republics and at a number of other administrative territories of the ussr in recent years is presented. s. flexneri has been shown to prevail at the territories with unsatisfactory water supply of the population, and s. sonnei prevails at the territories with good water supply. at the former territories s. dysenteriae and s. boydii retain their etiological importance, while at the la ...	1989	2698030
[development of antibiotic resistance of type 1 shigella dysenteriae strains (shiga bacillus) isolated in tananarive on the east coast of madagascar].	from november 1988 to march 1989, 804 malagasy children stools were studied and 37 shigella strains isolated. 5 shigella dysenteriae type 1 from malagasy east coast (mananjary), presented a multiply resistance to ampicillin, carbenicillin, streptomycin, chloramphenicol, tetracycline, sulphonamides and trimethoprim. this last resistance has recently appeared in this area.	1989	2699224
thromboxane synthase activity and platelet function after furegrelate administration in man.	furegrelate sodium (u-63,557a), a pyridine-derivative thromboxane synthase inhibitor, was administered orally in single doses of 200 to 1600 mg to normal male subjects. furegrelate produced a dose-related inhibition of thromboxane synthesis for 8-12 hours when measured either ex vivo from platelet-rich plasma (prp) or in vivo from urine. in general, the extent of thromboxane synthesis inhibition was greater in prp than in urine. furegrelate significantly inhibited platelet aggregation, but the e ...	1989	2708549
immunoblotting analysis of the reaction of wildebeest, sheep and cattle sera with the structural antigens of alcelaphine herpesvirus-1 (malignant catarrhal fever virus).	malignant catarrhal fever (mcf) is a disease of cattle and some other ruminants caused by alcelaphine herpesvirus-1 (ahv-1), a virus of wildebeest. the disease also occurs in the absence of wildebeest and is then thought to be caused by a viral agent harboured by the sheep. the structural proteins of ahv-1 have been used as antigens for the immunoblotting analysis of sera from wildebeest, sheep and cattle infected by either ahv-1 or the "sheep-associated" form of the disease. wildebeest sera sho ...	1989	2718352
fast elisa for measuring serum antibody responses.	a method which speeds up the enzyme-linked immunosorbent assay (elisa) is described. the procedure uses a modified falcon fast assay screening system (becton dickinson labware, lincoln park, nj) and falcon round-bottom 96-well plates. antigen is adsorbed onto beads which extend from a lid and fit into 96-well plates. the beads are washed in a trough and reacted to antibody in the round-bottom plate. the labor required to wash the plates after coating with antigen, antibody or conjugate is thereb ...	1989	2723439
analytical determination of methylated histidine in proteins: actin methylation.	the methylation of histidine in actin from various muscle and nonmuscle sources has been studied by formation of phenylthiocarbamyl derivatives and subsequent reverse-phase high-pressure liquid chromatographic separation and analysis of actin hydrolyzates. all the actin species examined were found to contain 3-methylhistidine. this method has also been used in assays for the enzyme(s) responsible for methylation of rabbit skeletal muscle actin and to investigate the formation of other methylated ...	1989	2729572
in vitro and in vivo comparison of the abilities of purine and pyrimidine 2',3'-dideoxynucleosides to inhibit duck hepadnavirus.	four purine and two pyrimidine 2',3'-dideoxynucleosides were studied for their ability to inhibit duck hepadnavirus replication. the purine 2',3'-dideoxynucleosides were more potent antiviral agents than the pyrimidine 2',3'-dideoxynucleosides. the concentration for 50% inhibition of viral replication (ic50) was determined for each of the effective agents. two drugs with low ic50s, 2,6-diaminopurine 2',3'-dideoxyriboside and 2',3'-dideoxyadenosine, were chosen for in vivo studies. animals receiv ...	1989	2729928
large phosphate shifts with treatment for hyperglycemia.	although hypophosphatemia is known to commonly accompany therapy for hyperglycemia, it is not generally appreciated that severe life-threatening depletion of phosphate may occur. i followed up two patients who had precipitous drops during intravenous insulin therapy for diabetic ketoacidosis and hyperosmolar nonketotic state. the patient with diabetic ketoacidosis had a phosphate level nadir that equaled the lowest recorded phosphate level in a living human (0.03 mmol/l). because (1) serum phosp ...	1989	2730261
relationship between multiple drug-resistance & enterotoxin production by shigella species.	culture filtrates prepared with strains of shigella dysenteriae 1 and those of sh. flexneri and sh. sonnei, collected from different geographical locations in india caused accumulation of fluid in rabbit gut loops, indicating their capability to produce shiga and shiga-like toxins respectively. sh. boydii strains were noted for the first time to produce shiga-like toxins. the failure of production of fluid accumulation in rabbit gut by shigella strains that lacked the r-markers of the antibiotic ...	1989	2737704
synaptic cytoarchitecture of the cat septum pellucidum.		1989	2747886
perioral anesthesia disrupts maternal behavior during early lactation in long-evans rats.	the role of perioral somatosensory stimulation from pups in maternal behavior was investigated in lactating long-evans rats (rattus norvegicus) during early lactation by local anesthesia of the mystacial pads with lidocaine, which blocks conduction in the infraorbital nerve. it was concluded that (1) acute perioral anesthesia markedly inhibited pup retrieval when the subjects had no formal pretreatment retrieval experience. further, these dams displayed little tolerance to the lidocaine treatmen ...	1989	2757582
relationship between concentration and anticonvulsant effect of phenytoin against electroshock-induced seizures in rats: comparison of sampling sites for concentration determinations.	the purpose of this investigation was to determine the optimum sampling site for phenytoin concentration measurements in the context of pharmacodynamic studies of the anticonvulsant effect of phenytoin. determination of drug concentrations in the serum, serum water, brain, and cerebrospinal fluid (csf) of rats as a function of time after iv injection of a 6-mg/kg dose revealed a significant disequilibrium between brain and serum water for 15 min and between csf and serum water for 5 min after in ...	1989	2760817
[infection caused by paracoccidioides brasiliensis in people living in cocle and veraguas, republic of panamá].	intradermal application of yeast phase paracoccidioidin antigen obtained by sonication and lysis from the fungus to 111 residents of veraguas province (68 men and 43 women) and 130 cocle province residents (57 men and 73 women) elicited a positive reaction in 9.01% (1/111) and 26.15% (34/30) of the veraguas and cocle residents respectively. positive reactions ranged between papules of 5 and 27 mm in diameter, the majority being between 11 and 20 mm. infection was more frequent in men (1:1.47 for ...	1989	2762594
determination of estradiol 2- and 16-alpha-hydroxylase activities in mcf-7 human breast cancer cells in culture using radiometric analysis.	using a radiometric assay the effects of estradiol upon the activity of estradiol 2- and 16 alpha-hydroxylases in mcf-7 human breast cancer cells in culture were studied. after 5 days of treatment and 36 h of withdrawal, incubation in the presence of either 2- or 16 alpha-tritiated substrate was carried out. estradiol (10 nm) significantly increased 16 alpha-hydroxylase activity (21%, p less than 0.01), while no effects on 2-hydroxylase activity was observed. treatment for 6 weeks caused a major ...	1989	2770303
twenty-year comparison of the human allograft and porcine xenograft.	this study compares a retrospective consecutive series of human allografts and concurrent porcine xenografts implanted over a 10-year interval. there were 571 allograft valves and 1,351 xenograft valves implanted in the aortic and mitral position with mean follow-up of 12.8 years for the allografts and 6.2 years for the xenografts. this study compares the incidence of structural deterioration over long-term follow-up. we found that there was no significant difference between four manufacturers o ...	1989	2774760
a strategy for high-volume sequencing of cosmid dnas: random and directed priming with a library of oligonucleotides.	direct sequencing of cosmid dnas using a library of oligonucleotide primers of length 8, 9, or 10 is proposed. the statistics of priming indicate that a primer library sufficient for determining the sequence of the entire human genome (100,000 cosmids) would be small enough to be assembled and managed. such a library would greatly reduce the cost and effort of high-volume sequencing: primers would be instantly available; the sequence of each cosmid dna could be determined from a single dna prepa ...	1989	2780548
neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a.	moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ...	1989	2783611
neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a.	moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ...	1989	2783611
co-localization of corticotropin-releasing factor- and enkephalin-like immunoreactivities in nerve cells of the rat hypothalamus and adjacent areas.	the co-localization of corticotropin-releasing factor (crf)- and enkephalin (enk)-like immunoreactivities in nerve cells of the rat hypothalamus and adjacent areas was investigated by the simultaneous application of immuno-beta-galactosidase staining and the peroxidase-antiperoxidase (pap) method to the same sections. crf-like immunoreactive cells were stained blue with immuno-beta-galactosidase staining and enk-like immunoreactive cells brown with the pap method. double-labeled cells with overl ...	1989	2786446
lymphokine-activated killer (lak) cells inhibit the clonogenic growth of human leukemic stem cells.	the effect of lymphokine-activated killer (lak) cells on the in vitro clonogenic capacity of acute myeloid leukemia (aml) blasts was investigated in a semisolid medium assay. the leukemic clonogenic capacity of 11 aml cases, selected on the basis of their ability to grow in vitro, was highly reduced following overnight preincubation with lak effectors. the degree of colony inhibition, which ranged between 66% and 98% (mean 83.8% +/- 11.4 sd), was quantitatively greater than by 51cr release, whic ...	1989	2786477
investigations of the potential for five beta-lactam antibiotics to elicit type ii hypersensitivity reactions in rats and monkeys.	immunologic reactions are occasionally elicited in patients by various beta-lactam antibiotics (e.g., penicillins and cephalosporins). a relatively rare reaction (type ii hypersensitivity) may involve antibody-mediated destruction of erythrocytes, leukocytes, and/or platelets. during the safety evaluation of several modified beta-lactam compounds (carbapenems), hemolytic anemia and/or neutropenia were observed in rhesus monkeys, and anemia, neutropenia, and thrombocytopenia in rats, after approx ...	1989	2786479
aging effects on secretory iga immune responses.	studies of cellular and humoral components of the secretory immune system indicated dramatic decreases in the siga response to t-dependent antigens (i.e. dnp-bgg) in aged rats. senescent rats (18-20 months old) showed comparable levels of siga anti-dnp antibody to adult animals after oral immunization with a t-independent antigen, dnp-ficoll. the salivary siga responses to both antigens were substantially decreased in weanling rats (21-35 days) versus the adult rats. significant anamnestic siga ...	1989	2786502
detoxification of lithocholic acid. elucidation of the pathways of oxidative metabolism in rat liver microsomes.	the hydroxylation of lithocholic acid (3 alpha-hydroxy-5 beta-cholanoic acid) by adult male sprague-dawley rat liver microsomes supplemented with nadph was studied. metabolites were separated by a combination of thin-layer chromatography and high pressure liquid chromatography, both with and without prior methylation and acetylation of the samples. the resulting products were characterized by thin-layer, gas-liquid, and high pressure liquid chromatography by comparison with authentic bile acid s ...	1989	2794781
experimental studies on membrane permeability defects of drug-resistant bacteria.	multiply antibiotic-, lysozyme-and bacitracin-resistant, representative strains of staphylococci, escherichia coli, shigella dysenteriae and klebsiella pneumoniae were compared respectively with their parent cultures in respect of their membrane permeability as judged by fluorescence polarization spectroscopy, using a sensitive fluorescent probe. the cytoplasmic membrane of the drug-resistant mutants was found to be less fluid and permeable, compared to that from the sensitive wild types. the st ...	1989	2807429
purification of shiga toxin and shiga-like toxins i and ii by receptor analog affinity chromatography with immobilized p1 glycoprotein and production of cross-reactive monoclonal antibodies.	shiga toxin from shigella dysenteriae 60r was purified to homogeneity by a novel one-step receptor analog affinity chromatography method. the method was based on the binding affinity of shiga toxin for a specific disaccharide, gal alpha 1----4gal, which was also present in glycoproteins with p1 blood group seroreactivity produced in hydatid cysts from sheep infected with echinococcus granulosus. having shown that cyst fluid p1 glycoprotein bound shiga toxin on a solid phase, a p1 glycoprotein af ...	1989	2807552
sex- and age-related changes in catecholamine metabolism and release of rat adrenal gland.	to examine the possible existence of changes in the adrenal catecholaminergic activity during aging, we analyzed the adrenal content of catecholamines (ca) and the activities of selected enzymes involved in their metabolism as well as the basal and the k+-stimulated release of these ca in incubated adrenal tissue of aged (greater than 22 months) and young (2 months) rats of both sexes. adrenal contents of norepinephrine (ne) and epinephrine (e) of male rats were unaltered in aging, although aged ...	1989	2812194
sex- and age-related changes in catecholamine metabolism and release of rat adrenal gland.	to examine the possible existence of changes in the adrenal catecholaminergic activity during aging, we analyzed the adrenal content of catecholamines (ca) and the activities of selected enzymes involved in their metabolism as well as the basal and the k+-stimulated release of these ca in incubated adrenal tissue of aged (greater than 22 months) and young (2 months) rats of both sexes. adrenal contents of norepinephrine (ne) and epinephrine (e) of male rats were unaltered in aging, although aged ...	1989	2812194
the renal phosphate transport defect in normal mice parabiosed to x-linked hypophosphatemic mice persists after parathyroidectomy.	the x-linked hypophosphatemic (hyp) mouse is a model for human x-linked hypophosphatemia. surgical joining of normal to hyp mice by parabiosis results in the normal mice developing low renal retention of phosphate and hypophosphatemia. these results suggest a humoral component to the renal defect. to test whether this component could be parathyroid hormone, surgical parathyroidectomy (ptx) or sham surgery was performed in mice 3 weeks after parabiotic union (n greater than 20 per group). after a ...	1989	2816501
improved sexual function in hemodialysis patients on recombinant erythropoietin: a possible role for prolactin.	as it was reported that correction of anemia in long-term hemodialysis patients by recombinant human erythropoietin (r-huepo) is associated with improved sexual function, we conducted the present study to further delineate the mechanism(s) by which this is brought about. serum prolactin, testosterone, and parathyroid hormone (pth) levels were followed during 4 months of r-huepo therapy. within 4 months of treatment with r-huepo, hematocrit values rose from 23.7 +/- 1.2 to 35.7 +/- 0.2% and hemog ...	1989	2914405
arthrodesis of metatarsophalangeal joints in a dog.	chronic osteomyelitis and septic arthritis involving the 3rd and 4th metatarsophalangeal joints were diagnosed in a doberman pinscher. arthrodesis of the 3rd and 4th metatarsophalangeal joints resulted in a subtle persistent gait abnormality, but limb function was preserved. arthrodesis of metatarsophalangeal joints is an alternative to amputation, especially when main weight-bearing digits are involved.	1989	2914800
the protective effect of thromboxane synthetase inhibition on renal function in systemic sepsis.	to study the role of thromboxane in systemic sepsis and renal failure, peritonitis was induced surgically in 22 sheep, leading to local and systemic sepsis. a selective thromboxane synthetase inhibitor, u63,557a (upjohn co, kalamazoo, mi) was given before surgery in five animals and 30 minutes after surgery in five animals. a typical picture of volume-loaded, normotensive, vasodilated septic shock developed in all animals. twenty four hours after induction of sepsis, the control group showed a m ...	1989	2916566
sensitivity of campylobacter pylori to colloidal bismuth subcitrate.		1989	2921366
three potassium currents in mouse motor nerve terminals.	a study of the k conductance of the presynaptic membrane has been performed in the triangularis sterni muscle of the mouse. external currents generated in the presynaptic terminals upon invasion by action potentials have been recorded using microelectrodes inserted into the perineurium of preterminal nerve bundles. the voltage-dependent k current could be pharmacologically dissected into fast (ikf) and slow (iks) components. while both are sensitive to 3,4-diaminopyridine (3,4-dap), only ikf is ...	1989	2928092
nontraditional pedagogy for implementing human genetics education.		1989	2929601
adp-ribosylatable content of elongation factor-2 changes during cell cycle of normal and cancerous human cells.	the amount of protein elongation factor ef-2 that can be inactivated by diphtheria toxin-mediated adp-ribosylation, a measure of its active content, decreases by 45% and 66% in g1-arrested normal human fibroblasts and in hela cells respectively. on restimulation of cells with fresh serum, the amounts of adp-ribosylatable ef-2 begin to increase within 4 h. whereas the level of active ef-2 returns to normal (exponential phase of growth) in 20 h in the case of fibroblasts, only 47% recovery was obs ...	1989	2930555
postoperative scars and cleavage lines.	we elucidated the pattern of skin cleavage lines of the neck of japanese cadavers and studied the scarring in 42 cases of radical neck dissection for the removal of metastatic nodules from the malignant tumors of the oral region, in the operation of which one incision was made parallel to, and another perpendicular to, the cleavage lines. from this study we came to the following conclusions: 1) the cleavage lines run parallel in ring-form around the neck, with the exception of the back and the l ...	1989	267516
[estrogen therapy and carcinoma of the endometrium (author's transl)].	the public discussion and the discussion among gynaecologists about the possible increased risk to develop carcinoma of the endometrium following post menopausal treatment with estrogens has lead to insecurity and restricted attitudes in the prescriptions of estrogens. in a statistic comparison of matched pairs of 130 cases of carcinoma of the endometrium with an equal number of selected control cases the following findings were elucidated: 1. any estrogen preparations do not increase the relati ...	1989	210085
primary aldosteronism-recognition and management.		1989	267288
relationship of oral complications to peripheral blood leukocyte and platelet counts in patients receiving cancer chemotherapy.	patients undergoing cancer chemotherapy often suffer from oral complications as a result of their disease and its treatment. the effects of the chemotherapy on the bone marrow and oral mucosa, coupled with the patient's immunosuppressed state and altered oral microbial flora, predispose these patients to oral mucositis, infection, and hemorrhage. the oral mucosa appears to mirror the effects of the chemotherapy on the bone marrow, as there appears to be a direct relationship between the changing ...	1989	313547
occlusal and craniofacial growth changes during puberty.		1989	267438
possible torsade in a patient with aids.		1989	2115530
[familial occurence of osteosarcoma (author's transl)].		1988	266820
[legal aspects: are the hands which provide care harmless].		1988	259575
inclusion vs. exclusion of the female in education of the male on the resumption of sex post first myocardial infarction.		1988	259408
[comparative data from radioisotopic and x-ray methods of study in osteogenic group tumors].	a comparative analysis of the data of roentgenography-angiography, scanning and radiometry with strontium-85 in 54 patients showing osteogenic sarcoma and osteoblastoclastoma has demonstrated a relationship between the quantitative characteristics of the radioactive sr inclusion and the intensity of reactive osteoformation, mineralization of neoplastic tissues, an activity of the pathological process, and the rate of vascularization, as well.	1988	266309
guidance on symptoms of illness in babies: a further service from the fsid.		1988	259593
mandibular staple bone plate implant and prosthesis. report of a case.	a case treated by the mandibular staple implant fastener system and prosthesis is presented. the phases of therapy, involving preprosthetic surgical preparation, construction of conventional dentures, implantation of the orthopedic appliance, preparation of an interim postsurgical prosthesis, and construction of the definitive prosthesis, are described and illustrated.	1988	266676
parallel studies of hla antigens in patients with rheumatic heart disease and scleritis: comparisons with three control populations.	parallel studies were carried out on hla antigens in patients with rheumatic heart disease and scleritis. comparison with one control population showed a significant excess of bw15 in both disease samples, while a comparison with two other control populations, showed the excess not to be significant. possible reasons for this discrepancy are discussed, together with the effect on statistical significance of a small percentage of false antigen assignments in one of the samples. a small systematic ...	1988	266606
hla b27 in ankylosing spondylitis: differences in frequency and relative risk in american blacks and caucasians.	twenty-eight hla alleles of the a and b loci were determined in 23 american blacks and 50 caucasians with primary ankylosing spondylitis (as). the prevalence of hla b27 was significantly increased in american black patients (48 per cent) vs black controls (two per cent), but was much less than the 94 per cent found in caucasian patients (controls eight per cent). the lower prevalence of b27 in american black patients vs caucasian patients was significant (p less than 0.001), and indicated that s ...	1988	266595
recent advances in genetic counselling.		1988	259242
clinical impact of drug resistance.		1988	2896697
a selective inhibitor of camp-specific phosphodiesterase, ro 20-1724, has no effect on the quantal release of acetylcholine from the mouse phrenic nerve.	the indirect twitch response of the mouse isolated phrenic nerve-diaphragm preparation, partially paralysed by tubocurarine, was restored only by about 10% by ro 20-1724 at 2 to 280 microm. the solvent vehicle, dimethylsulphoxide, also showed the same effect to a similar extent. intracellular recordings with glass microelectrodes revealed that ro 20-1724 (40 microm) affected neither the resting membrane potential, the amplitude and frequency of miniature endplate potentials nor the amplitude of ...	1988	2897454
dysentery due to multiresistant shiga bacillus in rural iran.		1988	2902359