Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| smoking during pregnancy--effects on the fetal thyroid function. | infants delivered at term by mothers smoking at least 10 cigarettes daily during pregnancy (n = 46) were found to be growth retarded compared to infants of non-smoking mothers (n = 49), birthweights 3,445 +/- 385 (sd) g and 3,667 +/- 392 g respectively (p less than 0.05) in the two groups. cord serum thyrotropin (tsh) was significantly decreased (8.2 +/- 4.0 u/l vs. 10.3 +/- 4.9 u/l) and free thyroxine index (ft4i)/tsh ratio significantly increased (18.8 +/- 9.0 vs. 14.4 +/- 7.6) (p less than 0. ... | 1986 | 3564944 |
| effects of thyroidectomy and administration of propylthiouracil (ptu) or thyrotropin (tsh) to pregnant rats on the functional development of the fetal thyroid gland an immunohistochemical study. | in order to elucidate the maternal factors influencing the functional development of the fetal rat thyroid gland, pregnant rats were subjected to either thyroidectomy or administration of ptu or tsh and the thyroid glands of the fetuses were examined chronologically by immunohistochemistry to detect thyroglobulin (tg), t4 and t3. in the group undergoing thyroidectomy, the occurrence of immunoreactive tg, t4 and t3 was the same as in the control group in spite of slight retardation of the develop ... | 1986 | 3556148 |
| effect of chronic dietary nitrate and different iodine supply on porcine thyroid function, somatomedin-c-level and growth. | due to the lately increased oral nitrate intake of humans and animals the influence of 3% kno3 in the diet on growth and the thyroid hormone and somatomedin-c-concentration in the serum was to be tested in an experiment with growing pigs in case of different iodine supply. the investigations were undertaken in 3 groups with 9 piglets each. the animals were 6 weeks old: 1. nitrate-exposed, 2. pair-fed to group 1 (without nitrate), 3. ad libitum without nitrate. the mean daily weight gains amounte ... | 1986 | 3556413 |
| a method for unidirectional deletion mutagenesis with application to nucleotide sequencing and preparation of gene fusions. | a method is described for the preparation of deletions that extend in one direction from a fixed point. the method is based on the ability of deoxynucleoside [1-thio]triphosphates to be incorporated into dna by dna polymerase i, klenow fragment, and the fact that alpha-thiophosphate-containing phosphodiester bonds are resistant to hydrolysis by the 3'-to-5' exonucleolytic activity of phage t4 dna polymerase. therefore, linear duplex dna molecules blocked at one 3'-terminus with a thiophosphate w ... | 1986 | 3552883 |
| the nucleotide sequence of gene 21 of bacteriophage t4 coding for the prohead protease. | we have sequenced gene 21 coding for the bacteriophage t4 prohead protease. the sequence codes for a protein of 212 amino acids (aa) with an mr of 23,251. a second possible in-frame initiation site was also found which would code for an mr 18,440 protein. evidence is presented that this second site is used in vivo. the only striking homology of gp21 to other proteins is with the serine proteases. the protein is homologous to a short aa sequence around the active site, but has a his where the act ... | 1986 | 3552886 |
| chemical synthesis of the t4 endonuclease v gene and its expression in escherichia coli. | a gene coding for t4 endonuclease v was constructed by joining chemically-synthesized oligodeoxyribonucleotides. this gene was inserted into an expression vector and expressed in e. coli. | 1986 | 3550706 |
| [intracellular development of escherichia coli bacteriophages after culturing the infected bacteria under conditions of anabiosis and hypothermia]. | the intracellular growth of bacteriophages t3, t4 and phi x174 was studied in escherichia coli cells frozen to -196 degrees c and cooled to 0 degree c at various intervals from the instant of phage infection. the processes of biosynthesis were delayed and the latent period was longer in the growth of cells frozen to -196 degrees c. the levels of rna and protein biosynthesis as well as the yield of phages decreased when cells were frozen at a later stage of the phage growth. no changes were found ... | 1986 | 3547045 |
| some characteristics of soluble thyroid proteins in human fetus during morphogenesis of follicular structure. | soluble thyroid proteins of the human fetus were studied in parallel with the formation of the follicular structure of the gland using polyacrylamide disc electrophoresis and a radioimmunological method for measuring thyroglobulin (tg). the study covered 24 fetuses obtained after sectio parvae performed at 12-28 weeks of gestation for socio-medical reasons. before the first trimester of gestation the human fetal thyroid, which did not have an organized follicular structure contained a significan ... | 1986 | 3549331 |
| [study of the circulating lymphocytes populations in systemic scleroderma, chronic discoid lupus erythematosus and dermatomyositis]. | the authors studied the peripheral blood lymphocytes in any autoimmune diseases. in scleroderma the t-lymphocytes and ratio t4/t8 are decreased and b-lymphocytes are normal. in the ledc the t-lymphocytes are decreased and b-lymphocytes are increased. in dermatomyositis the b-lymphocytes are increased and t-lymphocytes are decreased, the ratio t4/t8 is normal. | 1986 | 3543549 |
| [evaluation of real-time sonography in the pretherapeutic staging of malignant tumors of the tongue and mouth floor]. | based on our three years experience with about 200 transcutaneous real-time sonographies of the tongue and the floor of mouth, an assessment of the determination of size and site of 38 pretherapeutically examined malignant tumours (35 squamous cell carcinomas) was made. the patients were examined in reclined position, the head retroflected. curved array transducers of 5 or 7.5 mhz and a silicon elastomer block as interface turned out to be optimal. all tumours could be detected by sonography as ... | 1986 | 3543580 |
| the cellular composition of human lymph nodes after allogenic bone marrow transplantation: an immunohistological study. | using immunohistological techniques, the cellular composition of lymph nodes was assessed in 18 patients who had died 15 to 326 days after allogeneic bone marrow transplantation for leukaemia. the lymph nodes showed reduced cellularity of the cortex and paracortex, dilated sinuses and no lymphoid follicles. the majority of leucocytes were t lymphocytes with an inversion of the normal t4:t8 ratio. no cells were detected expressing immature cortical thymocyte antigens, using na1/34 and okt10, but ... | 1986 | 3543275 |
| t-zone lymphoma with predominance of 'plasmacytoid t-cells' associated with myelomonocytic leukaemia--a distinct clinicopathological entity. | in this paper we present a further case of a new clinicopathological entity combining a rare type of non-hodgkin lymphoma with a myelomonocytic leukaemia. the characteristic feature of the lymphoma is massive infiltration of the t-zones of lymph nodes by plasmacytoid cells originally described by lennert in non-specific lymphadenitis. two lymphoma cases of this type have recently been published, by müller-hermelink et al. who named the cells 'plasmacytoid t-cells' (ptc), and by prasthofer et al. ... | 1986 | 3543277 |
| zinc metalloproteins involved in replication and transcription. | rna polymerase (rpase) from e. coli contains two tightly incorporated zn(ii) ions, while the monomeric rpase from bacteriophage t7 does not contain zinc and does not require zn(ii) in the assay. one of the two zn(ii) ions can be differentially removed from e. coli rpase with p-hydroxymercuriphenylsulfonate (pmps) combined with edta and thiol. the resultant znl or zna rpase shows no alteration in transcription initiation and elongation rate from sigma-specific promoters. biosynthesis of a co2 rpa ... | 1986 | 3543219 |
| dynamics of recovery of body composition after overfeeding, food restriction or starvation of mature female rats. | in this study we examined the body composition of rats recovering from overfeeding, underfeeding or starvation. female rats (220 g) were fed 160%, 100% or 40% of control intake, by stomach tube, until the 40% rats had lost 50 g. other rats were starved to lose 50 g. carcass composition was measured on one group from each treatment. the remaining rats returned to ad libitum feeding. the 160% rats were hypophagic and lost weight. starved and 40% rats were hyperphagic and gained weight. serum insul ... | 1986 | 3543262 |
| immunofluorescent detection and localization of thyroxine in blood of adult amphibians. | indirect immunofluorescent staining was used to detect and localize thyroxine (t4) in blood smears from different species of adult amphibians, namely, rana pipiens, rana catesbeiana, bufo marinus, xenopus laevis, and notopthalmus viridescens. fluorescence, indicative of t4, was observed in both plasma and erythrocytes (rbc) from all individuals of the five species studied. it was weak and diffuse in the plasma and in the cytoplasm of the rbc but was intense in the nuclei (especially the nuclear ... | 1986 | 3542699 |
| autoantibodies to thyroid hormones: association with falsely low hormone levels measured by 'amerlex' assay. | autoantibodies to thyroxine (t4) and tri-iodothyronine (t3) were found in a 21-yr-old thyrotoxic woman who had paradoxically low t4 and t3 as measured in the 'amerlex' solid phase radio-immunoassay. though rare, thyroid hormone antibodies should be suspected if anomalous thyroid function results are obtained and alternate assay procedures used. | 1986 | 3542307 |
| phenotypic heterogeneity within the circulating human neonatal t4-positive t cell subset. | an indirect immunofluorescence staining technique was employed to evaluate the tq1 and 5/9 monoclonal antibody lymphocyte reactivity in 10 cord blood mononuclear cell (mc) preparations enriched of e-rosette-forming cells (e+). ten adult e+ mc populations were used as controls. unfractionated t4+ cord and adult mc positively selected by panning procedure were also assayed. the results of these experiments, taken together, suggest that there is an overexpanded neonatal t cell subset which displays ... | 1986 | 3542068 |
| effect of irradiation on serum t4 level and response of thyroid gland to exogenous tsh in rats. | 1986 | 3540283 | |
| use of monoclonal antibodies to human lymphocytes to identify lymphocyte subsets in lymph nodes of the rhesus monkey and the dog. | using commercially available monoclonal antibodies that bind to human lymphocyte subsets, we examined lymph nodes from the rhesus monkey and the dog for their binding ability to these monoclonal antibodies. the avidin biotin-peroxidase immunostaining procedure was used, and the following antibodies were reactive in the rhesus monkey: leu 4, leu 3a, okt4, leu 2a, okt8, t8, leu 5b, t11, leu 14, b1, and leu 12. no immunoreactivity was observed in the dog lymph node except for moderate reactivity of ... | 1986 | 3540307 |
| immunological typing of acute lymphoblastic leukemia: concurrent analysis by flow cytofluorometry and immunocytology. | for 60 cases of acute lymphoblastic leukemia (all) immunological typing was done concurrently by the avidin-biotin-peroxidase method using cytocentrifuged smears and by flow cytofluorometry for the study of surface antigens. the use of a large panel of antibodies detecting differentiation antigens allowed us to sub-classify 57/60 cases as 43 b-lineage alls and 14 t-lineage alls. the two types of all can be accurately distinguished by the expression of the antigens recognized by the antibodies of ... | 1986 | 3540462 |
| expression of the denv gene of coliphage t4 in uv-sensitive rad mutants of saccharomyces cerevisiae. | a plasmid containing the denv gene from bacteriophage t4, under the control of the yeast alcohol dehydrogenase i (adc1) promoter, conferred a substantial increase in uv resistance in the uv-sensitive saccharomyces cerevisiae mutants rad1-2 and rad3-2. the uv resistance of the denv+ yeast cells was cell cycle dependent and correlated well with the level of the denv gene product as measured by immunoblotting and by a photoreversal assay for pyrimidine dimer-dna glycosylase activity. | 1986 | 3540595 |
| thyroid function after surgical treatment of nontoxic goitre. a randomized study of postoperative thyroxine administration. | following thyroid resection for nontoxic goitre, 29 euthyroid patients were randomly allocated to no medication or to 0.2 mg levothyroxine daily (17 and 12 patients). the two groups were comparable in age, sex, extent of surgery and thyroid pathology. the patients were free from other endocrine disorders and had no other medication. serum tsh, t4 and t3 were measured and t3-resin test performed preoperatively and 14 days and 3, 6, 12 and 18 months postoperatively. all values were within normal r ... | 1986 | 3541502 |
| monoclonal antibody identification of infiltrating mononuclear leukocytes in lupus nephritis. | populations of mononuclear inflammatory cells infiltrating the renal interstitium in ln were studied by means of an avidin-biotin immunoperoxidase technique applied to cryostat sections of 26 renal biopsies (3 who class iib; 4 class iii; 8 class iv; 4 class v; 4 class iii and v; and 3 class iv and v). the majority of interstitial leukocytes were t cells (mean 65.7 +/- 14.1). the number of cells reactive with okt8 (47.3 +/- 11.0) exceeded the number of okt4 positive cells (32.5 +/- 11.3) in 22 of ... | 1986 | 3537453 |
| assembly of a trna splicing complex: evidence for concerted excision and joining steps in splicing in vitro. | splicing of trna precursors in saccharomyces cerevisiae extracts proceeds in two steps; excision of the intervening sequence and ligation of the trna halves. the ability to resolve these two steps and the distinct physical properties of the endonuclease and ligase suggested that the splicing steps may not be concerted and that these two enzymes may act independently in vivo. a ligase competition assay was developed to examine whether the excision and ligation steps in trna splicing in vitro are ... | 1986 | 3537690 |
| identifying leucocytes and leucocyte subpopulations in semen using monoclonal antibody probes. | an indirect immunoperoxidase technique employing specific monoclonal antibodies has been used to identify leucocyte subpopulations in cytocentrifuge smears of washed human ejaculate. cells reacting with the pan antihuman leucocyte monoclonal antibody (hle-1) were demonstrated in 63/67 specimens from subfertile patients with a mean count of 14.5 +/- 17.1 leucocytes per hpf (x 320). cells with similar reactivity were observed in all specimens examined from 10 fertile men with a mean count of 41.6 ... | 1986 | 3538613 |
| enumerating t-cell subsets on blood smears. an evaluation of an indirect immunoalkaline phosphatase method. | peripheral blood t-lymphocyte subsets in 179 chinese subjects were enumerated by an indirect immunoalkaline staining method done on ordinary blood smears. absolute counts (x 10(9)/l) were 1.32 +/- 0.53 (t11), 0.73 +/- 0.31 (t4), 0.45 +/- 0.22 (t8) for males (n = 135) and 1.22 +/- 0.39 (t11), 0.69 +/- 0.20 (t4), 0.44 +/- 0.19 (t8) for females (n = 44). t4:t8 ratio for males and females were 1.79 and 1.58, respectively (0.05 less than p less than 0.10). within-film area-to-area difference was 4.2 ... | 1986 | 3538846 |
| persistent generalized lymphadenopathy in drug addicts: immunological studies. | the persistent generalized lymphadenopathy (pgl) in drug addicts displays the same immunological abnormalities previously found in homosexual and haemophiliac men with pgl: impaired in vivo and in vitro t cell functions, inverted t4/t8 ratio in blood and b cell abnormalities. the peripheral blood b-lymphocytes, in fact, show a reduced in vitro immunoglobulin synthesis after pokeweed mitogen and staphylococcus aureus activation, with an increased spontaneous igg secretion. in the lymph node biops ... | 1986 | 3539149 |
| investigation of complexes formed between gene 32 protein from bacteriophage t4 and heavy-atom-modified single-stranded polynucleotides using optical detection of magnetic resonance. | optical detection of triplet-state magnetic resonance (odmr) is employed to study the complexes formed between gene 32 protein (gp32), a single-stranded dna-binding protein from bacteriophage t4, and the heavy-atom-derivatized polynucleotides poly(5-hgu) and poly(5-bru). the triplet-state properties of some of the tryptophan (trp) residues in the complexes are dramatically different from those in the free protein, in that they are subject to an external heavy-atom effect. direct evidence for the ... | 1986 | 3539180 |
| the cellular responses of tuberculosis and leprosy patients and of healthy controls in skin tests to 'new tuberculin' and leprosin a. | the density and distribution of t4 and t8 lymphocytes and of monocyte/macrophages at the site of skin tests with mycobacterial antigens was studied in pulmonary tuberculosis and leprosy patients and in healthy controls. most of the inflammatory cells were located in perivascular and periappendicular foci in the dermis: the percentage of the dermis occupied by focal infiltrate was unrelated to the clinical measurement of the area of induration. there was a less intense diffuse infiltrate in the d ... | 1986 | 3539419 |
| frameshift suppression by thya mutants of escherichia coli k-12. | we have extended our previous study on the suppression of frameshift mutants by escherichia coli thya mutants by assaying suppression of 15 riib frameshift mutants of bacteriophage t4 on one of our suppressing thya mutant strains. the majority of insertion mutants were suppressible, whereas none of the deletion mutants tested was suppressible. frameshift suppression could be inhibited by adding thymidine to the assay medium, but was not affected by the presence of a restrictive rpsl mutation in ... | 1986 | 3539695 |
| effect of fasting neonatal piglets on blood hormone and metabolite profiles and on skeletal muscle metabolism. | a pair of weight-matched piglets was identified at birth in each of six litters. one member of the pair was allowed to suckle for 24 h while the other was fasted. serum concentrations of insulin, thyroxine (t4), somatomedin-c (ria) and somatomedin-like activity (bioassay) were non-detectable or lower in non-suckled piglets when compared with suckled piglets. the converse situation was found for serum growth hormone (gh) levels, i.e., gh levels were higher (p less than .01) in non-suckled piglets ... | 1986 | 3539903 |
| feather and beak dystrophy and necrosis in cockatoos: clinicopathologic evaluations. | several species of imported and captive-bred southeast asian cockatoos with feather and beak disease (fbd) were evaluated. in recently emerging stained feathers from affected birds, intracytoplasmic magenta to basophilic inclusions of various sizes were found in macrophages and basophil-like cells within the pulp and feather epidermis. occasionally, amphophilic intranuclear inclusions were seen within degenerated feather epidermal cells. on the basis of electron microscopic findings, intracytopl ... | 1986 | 3506003 |
| plasma melatonin rhythm lost in preglaucomatous chicks. | domestic chicks (gallus domesticus) reared under continuous light, and thus developing light-induced avian glaucoma (liag), were tested at 4-hourly intervals around the clock, for evidence of hormonal or other rhythms which might be related to the eye effects of liag. plasma melatonin, corticosterone and thyroxine (t4) all displayed daily rhythms in the young chick reared under control (diurnal) lighting conditions. these daily hormone cycles were somewhat damped under either continuous light or ... | 1986 | 3503107 |
| reduced bone mass in calcitonin-deficient rats whether lactating or not. | calcitonin deficiency was produced in lactating and age-matched nonmated rats by thyroidectomy (tx) after transplantation of the parathyroid glands to a thigh muscle. at the end of lactation and a comparable period in the nonlactating rats, this condition resulted in femurs, vertebrae, and tibiae that weighed less than those in the thyroid-intact controls. furthermore, the femurs of the ct-deficient rats were narrower at midshaft and shorter, indicating reduced bone growth. the reduction in bone ... | 1986 | 3503538 |
| sensitization of t lymphocytes to thyroid antigen in autoimmune thyroid disease as demonstrated by the monocyte procoagulant activity test. | monocyte procoagulant activity (pca) production has been reported to have a close relation to cell-mediated immunity (cmi), and the collaboration of t lymphocytes is necessary to induce pca. antigen-specific sensitization of lymphocytes in patients with graves' disease (gd) and hashimoto's thyroiditis (ht) has been demonstrated by means of the production of cell-bound pca by monocytes following antigen stimulation of whole peripheral blood mononuclear cells (pbm). these cells, obtained from both ... | 1986 | 3494757 |
| [immunopathology of htlv iii infection in drug addicts]. | the authors are herewith reporting the results of an immunological screening performed on 15 drug addicts presenting serological positivity for anti htlv iii (elisa and wb). the study has been carried out determining populations and subpopulations of lymphocytes (t3, t4, t8 and t4/t8) and performing cutaneus tests with multiple antigens (tetanus--candida--diphtheria--proteus--streptococcus and tricophyton). the results achieved by mean of the above mentioned tests seem to indicate the presence o ... | 1986 | 3493791 |
| the t4 glycoprotein is a cell-surface receptor for the aids virus. | taken together, our studies suggest a mechanism of aids virus infection that initially involves the specific association of the aids virus with t4 molecules on the cell surface. this association does not require additional t-cell-specific molecules and can be demonstrated on both b lymphocytes and epithelial cell lines. the t4-aids virus complex is likely to be internalized in endosomes via receptor-mediated endocytosis. the virus can then fuse with the vacuolar membrane, releasing the viral nuc ... | 1986 | 3495395 |
| age-related anti-thyroid antibodies and thyroid abnormalities in turner syndrome. | one hundred pediatric patients with turner syndrome were studied to determine the correlation between the presence of anti-thyroid antibodies with age and karyotype, and the value of anti-thyroid antibody titers as predictors of subsequent thyroid abnormalities. (54 patients = 45,x; 46 = other karyotypes.) the frequency of positive titers of anti-thyroid antibodies increased linearly with cumulative age. anti-parietal cell and anti-adrenocortical cell antibodies were not increased in these patie ... | 1986 | 3494376 |
| reactivity of monoclonal antibody be 2 in different stages of mycosis fungoides and in benign dermal infiltrates. | the cutaneous infiltrate in mycosis fungoides (mf) is predominantly composed of t4-positive t-cells. attempts to distinguish the early stages of this condition from benign inflammatory infiltrates using anti-t3, t4 and other t-cell-associated antibodies have hitherto been unsuccessful. recently a monoclonal antibody be 2 has been described as selectively reacting against leukemic cells in patients with cutaneous t-cell lymphoma. to investigate whether the be 2 antigen is differentially expressed ... | 1986 | 3494432 |
| clinical and immunologic sequelae of aids retrovirus infection. | the sydney aids project is a prospective immunoepidemiological study of 911 homosexual and bisexual men enrolled between february 1984 and january 1985. clinical, immunological, and serological studies are performed on these subjects every six months. at enrollment, 39.9% of subjects were seropositive for antibodies to aids retrovirus (arv). of these 352 seropositive subjects, 28.1% were symptomless with normal immune profiles, 23.6% were symptomless with an immunodeficiency, 18.8% had a clinica ... | 1986 | 3494440 |
| effects of thyroid hormone, stalk section, and transplantation of the pituitary gland on plasma prolactin levels at metamorphic climax in rana catesbeiana. | plasma prolactin (prl) concentration increases markedly at late metamorphic climax (stage xxiv of taylor and kollros (1946)) in rana catesbeiana. two series of experiments were performed in order to elucidate the mechanisms involved in this phenomenon. first, thyroidectomized tadpoles (stages xvi-xviii) were kept in tap water with or without thyroxine (t4). tadpoles in tap water did not develop beyond stage xx and showed no sign of hypersecretion of prl by the time t4-treated tadpoles reached st ... | 1986 | 3493949 |
| observations on the interaction of prolactin and thyroxine in the tail of the bullfrog tadpole. | explants of tail fin from tadpoles of rana catesbeiana maintained in tissue culture underwent resorption when exposed to a medium containing 150 nm thyroxine (t4). increases in the specific activity of hexosaminidase (hex) and of acid phosphatase (ap) were associated with this response. ovine prolactin inhibited resorption of explants in a dose-responsive manner; however, prolactin had no influence on the specific activity of either hex or ap cultured explants. this result contrasted with the ef ... | 1986 | 3493950 |
| translational repression in vitro by the bacteriophage t4 rega protein. | the bacteriophage t4 translational repressor rega protein has been purified from an overproducing strain, and its activity has been studied in simple in vitro protein synthesis reactions. rega protein was found to inhibit the translation of t4 genes 44, 45, and orf45-1 in a concentration-dependent fashion. expression of two other t4 genes which are insensitive to rega protein in vivo, genes 32 and 43, was unaffected by the presence of rega protein. specific inhibition of synthesis of genes 44, 4 ... | 1986 | 3502424 |
| the pathology and treatment of interstitial pneumonitis in two infants with aids. | two infants with aids who presented with interstitial pneumonitis, failure to thrive, lymphadenopathy, and hypergammaglobulinemia have been studied. antibody to human t-lymphotropic retrovirus (htlv-iii) was identified by elisa and western blot analysis in serum samples from both patients. the t4/t8 ratios of peripheral blood t-lymphocytes in both patients were mildly decreased, with normal absolute numbers of lymphocytes and positive t4 cells. lung biopsies from both patients demonstrated simil ... | 1986 | 3509145 |
| the stimulation of sugar transport in heart cells grown in a serum-free medium by picomolar concentrations of thyroid hormones: the effects of insulin and hydrocortisone. | chick embryo heart cells were propagated in a defined serum-free medium. they formed a confluent, synchronously contracting monolayer that is not different from myocytes grown in serum containing media. the uptake of 2-deoxy-d-[1-3h]glucose in these cells was stimulated by exposure to physiological concentrations of t3 (1 pm) and t4 (10 pm). actinomycin-d and puromycin did not block the stimulation of 2-deoxy-d-[1-3h]glucose uptake when given with t3 throughout a 6-h incubation period. cells gro ... | 1986 | 3510123 |
| genotype dependency of the thermic effect of a meal and associated hormonal changes following short-term overfeeding. | the purpose of this experiment was to assess the effects of short-term overfeeding (mixed diet) on the thermic effect of a meal (tem) and associated hormonal changes and to investigate the role of the genotype in the observed changes. six pairs of male sedentary monozygotic (mz) twins consumed an extra 1,000 kcal per day over their individual level of energy expenditure while maintaining a sedentary existence. resting metabolic rate (rmr) and tem following a 4.2 mj meal challenge was measured be ... | 1986 | 3510362 |
| reduction and repopulation of recipient t4+ and t8+ t-lymphocytes in allogeneic bone marrow transplantation. | in eight recipients of allogeneic bone marrow grafts who had sex-mismatched donors, the reduction and subsequent repopulation of t4+ and t8+ t-lymphocytes of recipient origin were studied. the origin of the donor-recipient t4+ and t8+ t cells was studied using quinacrine staining of y chromatin combined with t-cell typing for t4 and t8. following chemoradiotherapy and bone marrow transplantation (bmt), t cells reached their nadir at a median of five (range 1-8) days after bmt. t8+ t cells decrea ... | 1986 | 3510894 |
| thymine glycol lesions terminate chain elongation by dna polymerase i in vitro. | single-strand circular dna from bacteriophage m13mp9 was chemically modified with osmium tetroxide to introduce specifically cis-thymine glycol lesions, a major type of dna damage produced by ionizing radiation. an oligonucleotide primer was extended on damaged and undamaged templates using either the large fragment of e. coli pol i or t4 dna polymerase. the reaction products were analysed by electrophoresis alongside a dna sequence ladder. synthesis on the damaged templates terminated at positi ... | 1986 | 3511447 |
| long-term endocrinologic changes in subjects practicing the transcendental meditation and tm-sidhi program. | the transcendental meditation (tm) and a more advanced program, the tm-sidhi program, have been reported to produce a number of acute and long-term metabolic and electrophysiologic changes. to investigate the possibility that the practice of these techniques may be associated with long-term endocrinologic changes, we prospectively evaluated 11 male subjects before and over a 3-year period after starting the tm-sidhi program. a progressive decrease in serum tsh, growth hormone, and prolactin leve ... | 1986 | 3511493 |
| mononuclear cell subpopulations in the skin defined by monoclonal antibodies after hla-identical sibling marrow transplantation. | mononuclear cell subpopulations present in the skin of 36 recipients of hla-identical sibling marrow transplants were defined by immunoperoxidase using a battery of monoclonal antibodies to cell surface differentiation antigens. the t4-positive (t4+) (helper-inducer t cells), t8+ (cytotoxic-suppressor t cells) and the t6+ (langerhans cells) decreased in number early post transplant and returned towards normal numbers from day 42 onwards. there was no evidence that either the t4+ or the t8+ subse ... | 1986 | 3511942 |
| enumeration of absolute numbers of t lymphocyte subsets in b-chronic lymphocytic leukaemia using an immunoperoxidase technique: relation to clinical stage. | an immunoperoxidase technique has been used to identify and enumerate helper and suppressor t-cell subsets, as defined by reactivity with coulter t4 and okt8 monoclonal antibodies in 54 patients with b chronic lymphocytic leukaemia (b-cll) and in the same number of matched controls. the ratio of t4+ to t8+ cells was significantly reduced in the b-cll group as a whole (p less than 0.001) and in each stage of the three clinical staging systems. there was an increase in the median absolute level of ... | 1986 | 3511946 |
| influence of graft-versus-host disease prophylaxis on early t-lymphocyte regeneration following allogeneic bone marrow transplantation. | an early decrease in the ratio between t4+ and t8+ t lymphocytes has been shown to correlate with the development of grade ii-iv gvhd in allogeneic bone marrow transplant (bmt) recipients receiving methotrexate (mtx) as prophylaxis for acute graft-versus-host disease (gvhd). this study compares the onset of t-cell regeneration in patients receiving cyclosporin a (cya) with those receiving mtx. firstly, lymphoid recovery occurred at a significantly faster rate in the patients on cya. secondly, in ... | 1986 | 3511949 |
| kinetics of protein-nucleic acid interactions: use of salt effects to probe mechanisms of interaction. | the kinetics of protein-nucleic acid interactions are discussed with particular emphasis on the effects of salt concentration and valence on the observed rate constants. a general review is given of the use of experimentally determined salt dependences of observed kinetic parameters as a tool to probe the mechanism of interaction. quantitative analysis of these salt dependences, through the application of polyelectrolyte theory, can be used to distinguish reactions which occur in a single step f ... | 1986 | 3512164 |
| physical discrimination between human t-lymphocyte subpopulations by means of light scattering, revealing two populations of t8-positive cells. | light-scattering properties of human t-lymphocyte subpopulations selected by immunofluorescence were studied. based on differences in orthogonal light scattering, two subpopulations of t8-positive cells can be distinguished. the first population (t8a) has the same orthogonal light-scattering properties as t4-positive cells, whereas the orthogonal light scattering of the second population (t8b) was about 70% larger. orthogonal light scattering of leu7-positive lymphocytes resembles that of the t8 ... | 1986 | 3512198 |
| reduction and repopulation of t-lymphocytes after cytoreductive therapy with or without autologous bone marrow rescue. | the reconstitution of peripheral-blood t-lymphocytes following cytoreductive therapy in standard (11 patients) or in high dosages (ten patients) was compared with that after supralethal cytoreductive therapy followed by autologous bone marrow rescue (abmr, 20 patients). along with the increasing cytotoxic potential of the three therapy protocols, t-cell counts fell to lower levels. following all three forms of cytoreductive therapy, t8+ t-cell counts decreased to lower levels than t4+ t-cell cou ... | 1986 | 3512277 |
| direct role of the escherichia coli dam dna methyltransferase in methylation-directed mismatch repair. | the t4 dam+ gene has been cloned (s. l. schlagman and s. hattman, gene 22:139-156, 1983) and transferred into an escherichia coli dam-host. in this host, the t4 dam dna methyltransferase methylates mainly, if not exclusively, the sequence 5'-gatc-3'; this sequence specificity is the same as that of the e. coli dam enzyme. expression of the cloned t4 dam+ gene suppresses almost all the phenotypic traits associated with e. coli dam mutants, with the exception of hypermutability. in wild-type hosts ... | 1986 | 3512529 |
| monoclonal antibodies distinguish titins from heart and skeletal muscle. | murine monoclonal antibodies specific for titin have been elicited using a chicken heart muscle residue as antigen. the three antibodies t1, t3, and t4 recognize both bands of the titin doublet in immunoblot analysis on polypeptides from chicken breast muscle. in contrast, on chicken cardiac myofibrils two of the antibodies (t1, t4) react only with the upper band of the doublet indicating immunological differences between heart and skeletal muscle titin. this difference is even more pronounced f ... | 1986 | 3512578 |
| a recombinant 64 kilodalton protein of mycobacterium bovis bacillus calmette-guerin specifically stimulates human t4 clones reactive to mycobacterial antigens. | a recombinant 64 kd protein of mycobacterium bovis bacillus calmette-guerin (bcg) (antigen a), which amounted to approximately 2% of an e. coli lysate, was tested for its capacity to stimulate human t4 clones reactive to mycobacterial proteins. two out of four crossreactive clones, established from a patient with tuberculoid leprosy, which could be stimulated by protein preparations of m. leprae and m. tuberculosis, and by particulate m. bovis bcg were also reactive to antigen a without further ... | 1986 | 3512760 |
| specific inhibition of mrna translation by complementary oligonucleotides covalently linked to intercalating agents. | synthetic oligodeoxynucleotides that are covalently linked at their 3' end to an acridine derivative and are complementary to the repeated sequence uuaaauuaaauuaaa adjacent to the ribosome binding site of the gene 32-encoded mrna from phage t4 have been used to regulate the synthesis of gene 32-encoded protein in vitro. these modified, synthetic oligonucleotides specifically block the translation of gene 32-encoded mrna with a higher efficiency than the homologous unsubstituted oligonucleotides. ... | 1986 | 3513172 |
| dependence of ms2 and t4 phage growth upon host amino acid biosynthesis during infections of escherichia coli. | by manipulating the growth conditions of escherichia coli both before and after phage ms2 or phage t4 infections, a dependence of phage growth upon postinfection host amino acid biosynthesis can be inferred. cells grown under repressing conditions for amino acid biosynthesis shifted to amino acid-free medium postinfection (in the absence of further host gene expression) are poor hosts for phage growth, whereas cells grown under derepressing conditions for amino acid biosynthesis are good hosts r ... | 1986 | 3513437 |
| thyroid cartilage imaging with diagnostic ultrasound. correlative studies. | in 1976, noyek observed that the thyroid cartilage framework of the larynx could be imaged by diagnostic ultrasound; the image appeared uniquely independent of the presence or absence of calcification. it was suggested that ultrasound, with its simple, nonroentgenographic, noninvasive features, might permit screening detection of thyroid cartilage destruction. it might, therefore, give direction to better computed tomographic (ct) imaging, and, specifically, more effectively identify t4 laryngea ... | 1986 | 3513800 |
| eczematous reactions in atopic patients caused by epicutaneous testing with inhalant allergens. | to determine whether inhalant allergens could induce eczematous lesions we studied 17 patients with atopic eczema (with or without allergic rhinitis), 13 patients with allergic rhinitis without atopic eczema and 10 healthy control subjects. the allergens, birch pollen (betula verrucosa) and house dust mite (dermatophagoides pteronyssinus), were applied in aluminium chambers for 48 h on clinically normal skin. in 17 patients with atopic eczema, six epicutaneous test reactions of the delayed type ... | 1986 | 3513815 |
| unpaired cysteine-54 interferes with the ability of an engineered disulfide to stabilize t4 lysozyme. | we have introduced an intramolecular disulfide bond into t4 lysozyme and have shown this molecule to be significantly more stable than the wild-type molecule to irreversible thermal inactivation [perry, l.j., & wetzel, r. (1984) science (washington, d.c.) 226, 555-557]. wild-type t4 lysozyme contains two free cysteines, at positions 54 and 97, and no disulfide bonds. by directed mutagenesis of the cloned t4 lysozyme gene, we replaced ile-3 with cys. oxidation in vitro generated an intramolecular ... | 1986 | 3513834 |
| alignment and merging of electron microscope images of frozen hydrated crystals of the t4 dna helix destabilizing protein gp32*i. | low dose cryoelectron microscopy has been used to record images and electron diffraction patterns of frozen hydrated crystals of the single-stranded dna binding protein gp32*i. fourier transforms from 13 image areas, corresponding to approximately 40,000 unit cells, were aligned by a minimal phase residual search and merged by vector addition in reciprocal space. phases from the resulting composite transform were combined with amplitudes from electron diffraction patterns to reconstruct the proj ... | 1986 | 3513856 |
| frameshift suppression in aminoacyl-trna limited cells. | under certain conditions aminoacyl-trna limitation can phenotypically suppress frameshift alleles. the observed suppression is due to an increase in abnormal translocation of ribosomes translating codons that have a short supply of aminoacyl-trna. the riib frameshift alleles of bacteriophage t4 are used here to pinpoint the sites of ribosome frameshifting caused by these hypothetical decoding errors. the data indicate that not all hungry codons are associated with abnormal translocation, only a ... | 1986 | 3514372 |
| erythema multiforme in a patient with t cell chronic lymphocytic leukemia. | a patient with t4+ (helper-inducer) t cell chronic lymphocytic leukemia developed an erythema multiforme-like eruption, the diagnosis of which was supported by routine light microscopic findings. immunopathologic studies using monoclonal antibodies demonstrate that despite an overwhelming majority of leukemic t4+ cells in the peripheral blood and dermal infiltrate, the predominant cells in the epidermal infiltrate are t8+ (cytotoxic-suppressor) cells. these findings are different from those seen ... | 1986 | 3514707 |
| immunohistochemical demonstration of myoglobin (mb) in pathological thyroid tissue. correlation with thyroglobulin (tg), thyroxine (t4) and triiodothyronine (t3) distribution. | myoglobin (mb) was investigated by the immunoperoxidase method (pap) in 48 cases of thyroid diseases including euthyroid goiter, graves' disease, folliculo-papillary carcinoma, follicular carcinoma, anaplastic carcinoma and medullary carcinoma. mb was demonstrated in most of the pathological thyroid tissues. in benign lesions, mb is diffusely disposed throughout the cytoplasm in moderate quantities and only occasionally predominates at the apical border of thyreocytes. generally, in differentiat ... | 1986 | 3515507 |
| purification and characterization of bacteriophage 9na lysozyme. | bacteriophage 9na is a virulent phage of salmonella typhimurium which induces a lysozyme in host cells toward the later stages of its multiplication. 9na lysozyme has been purified about 1000 fold starting from the lysate of 9na infected cells. the enzyme has an optimum ph between 7 and 8 and its activity is dependent on the ionic strength of the assay medium. salts like nacl and kcl are inhibitory to the lysozyme. gram-negative cells act as better substrate for the lysozyme than do gram-positiv ... | 1986 | 3516153 |
| influence of thyroid hormones on rat cerebellar cell aggregation and survival in culture. | in order to study the effect of thyroid hormones on rat cerebellar granule cells, cerebellar perikarya isolated from 5-7-day-old rats were cultured in the presence of normal fetal calf serum, fetal calf serum treated with dowex-2 ion exchange resin to remove thyroid hormones, or ion exchange-treated serum resupplemented with physiological concentrations of t4 and t3. granule cells grown under hypothyroid conditions showed abnormal cellular aggregation, neurite fasciculation and cell survival. ce ... | 1986 | 3516314 |
| [determination of thyroid volume using computerized ultrasonography and its clinical application]. | a new method for determining thyroid gland volume was developed by means of computerized ultrasonography. a maximum area of the thyroid (s) was obtained from coronal sections and a maximum thickness (c) was obtained from sagittal sections. the thyroid volume was then calculated by sxc. the volume of phantoms and pig thyroids obtained by this method was identical with the actual volume. the thyroid volume in this method correlated well with the estimated volume in patients with graves' disease un ... | 1986 | 3516734 |
| possible role of pancreatic insulin and glucagon in the hyperlipidemia and obesity of obese strain of chicken. | circulating levels of insulin, glucagon, thyroid hormones as well as lipid levels were determined in an obese strain of chicken and their lean controls. hepatic and muscle glycogen and lipids were also measured. obese birds had higher plasma lipids accompanied by significantly higher insulin and lower glucagon levels compared to lean controls. hepatic and muscle triglycerides were also higher in obese birds. plasma t4 level was significantly higher in obese but t3 was not different in the two gr ... | 1986 | 3516830 |
| early results of the eortc randomized clinical trial on multiple fractions per day (mfd) and misonidazole in advanced head and neck cancer. | from feb. 1981 to oct. 1984, a randomized clinical trial was carried out in the eortc radiotherapy group, comparing classical radiotherapy 70 gy/7 weeks to mfd (3 x 1.6 gy/day for 10 days, 3 weeks rest, followed by a boost to 67.2 or 72 gy), with or without misonidazole (1 g/m2 every irradiation day, total 12 to 14 g/m2) in advanced head and neck cancer (all t3 and t4, all n2, n3 and n1 greater than 3 cm). a total of 523 patients were entered in the study. at the time of analysis (4/85), the med ... | 1986 | 3516953 |
| effects of obesity on insulin and glucose metabolism in cyclic heifers. | effects of degree of obesity on basal concentrations of insulin, glucose, thyroxine (t4), triiodothyronine (t3), estradiol-17 beta (e) and progesterone (p) were measured in serum from 50 estrous and 73 diestrous holstein heifers and the insulin response to glucose infusion was assessed in diestrous obese (n = 7) and lean (n = 7) heifers. basal concentrations of glucose, t4, t3, e and p were not correlated with degree of obesity, although concentrations of glucose, t4 and t3 were higher (p less t ... | 1986 | 3516960 |
| [physiology of langerhans cells and their potential role in oral pathology]. | langerhans cells represent a minor epidermal cell population in mammals. they are also observed in squamous epithelia of the oesophagus, vagina and cervix, as well as in oral epithelia. they occur in higher density in the non keratinized epithelium. these cells are characterized by a dendritic pattern, a clear cytoplasm and ultrastructurally by the presence of birbeck granules. they are usually located in a suprabasal position. their bone marrow origin is now well established. surface markers an ... | 1986 | 3516986 |
| the relative cytotoxicity and mutagenicity of cyclobutane pyrimidine dimers and (6-4) photoproducts in escherichia coli cells. | in order to calculate the relative cytotoxicity and mutagenicity of (5-6) cyclobutane pyrimidine dimers and (6-4) photoproducts, we have measured survival and mutation induction in uv-irradiated excision-deficient e. coli uvra cells, with or without complete photoreactivation of the (5-6) dimers. radioimmunoassays with specificity for (5-6) dimers or (6-4) photoproducts have shown that maximum photoreactivation eliminates all of the (5-6) dimers produced up to 10 jm-2 254-nm light, while it has ... | 1986 | 3517633 |
| persistence of dna synthesis arrest sites in the presence of t4 dna polymerase and t4 gene 32, 44, 45 and 62 dna polymerase accessory proteins. | dna synthesis by phage t4 dna polymerase is arrested at specific sequences in single-stranded dna templates. to determine whether or not t4 dna polymerase accessory proteins 32, 44, 45 and 62 eliminated recognition of these arrest sites, unique primer-templates were constructed in which dna synthesis began at a dna primer located at different distances from palindromic and nonpalindromic arrest sites. nucleotide positions that caused polymerase to pause or leave the template were identified by s ... | 1986 | 3517810 |
| ovarian hormone effects on activity, glucoregulation and thyroid hormones in the rat. | ovarian hormonal influences on the range of physiological and behavioral variables which combine to affect overall energy balance are poorly delineated. in the present study 4 groups of virgin, female rats (intact, ovariectomized, ovariectomized with estrogen replacement and ovariectomized with estrogen plus progesterone) were allowed access to running wheels and activity; food intake and weight gain were measured initially under food restricted, then under ad lib conditions. serum insulin, gluc ... | 1986 | 3517902 |
| cis-diamminedichloroplatinum (cddp)-fosfomycin (fom) combination in lung cancer patients with respect to the peripheral t lymphocyte subsets. | an antibiotic fom was found to be preventive of the side effect of an antineoplastic drug cddp on the peripheral t lymphocytes in lung cancer patients. whereas finding a significant decrease in t3+ cells (p less than 0.01) and t4+ subset (p less than 0.05) with cddp alone, the initial levels of these cell populations were kept unchanged in combination with fom. t8+ subset showed no substantial change. a protective effect of fom on t4+ subset was suggested to be an additional aspect of its action ... | 1986 | 3518153 |
| [possibility of evaluating the degree of dissemination of malignant tumors of the bladder using ultrasonic tomography]. | the paper discusses the results of an analysis of degree of bladder cancer expansion with the aid of ultrasonic tomography. the results of tomography were compared with surgical and pathohistologic findings. the study group included 94 patients with bladder cancer. stages a, b, and c were distinguished on the basis of tomographic features, which correspond to t1, t2, t3 and t4 according to tnm system. application of ultrasonic tomography in conjunction with other diagnostic procedures offers mor ... | 1986 | 3518238 |
| evaluation of lymphocyte subsets after autologous bone marrow transplantation with marrow treated by asta z 7557 in acute leukemia: incidence of the in vitro treatment. | the lymphocyte subset reconstitution after high-dose chemotherapy and total body irradiation followed by autologous bone marrow transplantation (abmt) has been studied in ten patients with acute leukemia (al) (6 all and 4 anll) in complete remission (cr). bone marrow was treated in vitro with high-dose asta z 7557, individually determined according to cfu-gm sensitivity. the different peripheral blood lymphocyte subsets were characterized by means of monoclonal antibodies (indirect immunofluores ... | 1986 | 3519264 |
| factors predicting the course of diabetes mellitus in hyperthyroid patients. | the relationship between changes in glucose tolerance with treatment of hyperthyroidism and various factors that might be relevant to carbohydrate metabolism were investigated in 64 hyperthyroid patients with abnormal glucose tolerance, including 35 cases with fasting plasma glucose (fpg) levels of 140 mg/dl or more. all patients had diffuse toxic goiter. after correction of the hyperthyroidism, glucose intolerance improved in almost all cases, even in cases with fasting hyperglycemia, but diabe ... | 1986 | 3519414 |
| inhibition of iodothyronine transport into rat liver cells by a monoclonal antibody. | the role of the rat liver plasma membrane in the regulation of uptake and subsequent deiodination of thyroxine (t4) or the biologically active thyroid hormone 3,3',5-triiodothyronine (t3) was investigated. here we report on the production of monoclonal antibodies raised against rat hepatocytes. two antibodies were selected. antibody er-22 did bind to a mr 52,000 membrane protein and inhibited the 1- and 5-min uptake of both t4 and t3 by primary cultured rat hepatocytes in a dose-dependent fashio ... | 1986 | 3519612 |
| glucoregulatory function of thyroid hormones: interaction with insulin depends on the prevailing glucose concentration. | the effect of elevated serum thyroid hormone concentrations on insulin-induced glucose metabolism was studied in healthy subjects before and after t4 administration (250 micrograms t4/day for 10-14 days). this treatment induced moderate hyperthyroidism (t4, 15.2 micrograms/dl; t3, 200 ng/dl). the following results were obtained. insulin receptor binding to a 90% enriched monocyte fraction or to mitogen-stimulated cultured t lymphocytes was decreased by t4 administration, whereas insulin binding ... | 1986 | 3519650 |
| iodothyronine deiodination in the brain of diabetic rats: influence of thyroid status. | experimental diabetes causes profound alterations in the metabolism of thyroxine (t4), including a decrease in hepatic triiodothyronine (t3) generation from t4 via 5'-deiodination (5'-d). because 5'-d in brain differs markedly from that in liver, both in enzymatic mechanism and in the response to hypothyroidism, we studied iodothyronine deiodination, in particular t4 to t3 conversion (t4-t3), by incubating 125i t4 with particulate fractions of cerebral cortex (cx) and cerebellum (cm) from rats m ... | 1986 | 3519744 |
| a lymphokine that activates the cytolytic program of both cytotoxic t lymphocyte and natural killer clones. | a 10-12 kd lymphokine, herein termed tcaf, was recently shown to be secreted from th after crosslinking of their antigen/mhc (t3-ti) receptors. tcaf stimulates resting t lymphocyte proliferation via binding to surface components of the t11 pathway. to determine whether tcaf could induce antigen-independent activation of the lytic machinery of cytotoxic cells, the present studies were conducted. in the presence of tcaf, both t8+ class i mhc-specific and t4+ class ii mhc-specific cytotoxic t cell ... | 1986 | 3519831 |
| phage and host genetic determinants of the specific anticodon loop cleavages in bacteriophage t4-infected escherichia coli ctr5x. | anticodon loop cleavages of two host trna species occur in bacteriophage t4-infected escherichia coli ctr5x, a host strain restricting phage mutants deficient in polynucleotide kinase (pnk) or rna ligase (rli). the cleavage products accumulate with the mutants but are further processed in wt infection through polynucleotide kinase and rna ligase reactions. inactivating mutations in stp suppress pnk- or rli- mutations in e. coli ctr5x and, as shown here, also abolish the anticodon nuclease, impli ... | 1986 | 3519981 |
| assembly and disassembly of bacteriophage t4 polyheads. | the assembly of the product of bacteriophage t4 gene 23 (gp23), the uncleaved form of the main shell protein, has been studied. assembly and disassembly follow the predictions for entropy-driven processes; assembly is strongly favored by conditions of high salt concentrations and high temperatures, whereas low salt and low temperatures promote disassembly. in the absence of the scaffolding core proteins in vitro, only polyheads, the tubular variant of the prohead, are produced. kinetic studies s ... | 1986 | 3519982 |
| identification of t4 gene 25 product, a component of the tail baseplate, as a 15k lysozyme. | the proteins synthesized in escherichia coli b cells after infection with various t4 bacteriophage tail baseplate mutants were analysed by the immunoblotting method for the presence of the 15 kilodalton lysozyme found in phage t4 particles. using three different antisera: anti-phage, anti-baseplate and anti-15k lysozyme, it has been found that the 15k lysozyme is not present in lysates of bacteria infected with t4 gene 25 amber mutants. the 15k lysozyme was also found to be expressed in e. coli ... | 1986 | 3520236 |
| partial complementation of the uv sensitivity of e. coli and yeast excision repair mutants by the cloned denv gene of bacteriophage t4. | the denv gene of bacteriophage t4 was reconstituted from two overlapping dna fragments cloned in m13 vectors. the coding region of the intact gene was tailored into a series of plasmid vectors containing different promoters suitable for expression of the gene in e. coli and in yeast. induction of the tac promoter with iptg resulted in overexpression of the gene, which was lethal to e. coli. expression of the tacdenv gene in the absence of iptg, or the use of the yeast gal1 or adh promoters resul ... | 1986 | 3520242 |
| prophylactic use of okt3 monoclonal antibody in cadaver kidney recipients. utilization of okt3 as the sole immunosuppressive agent. | we describe the first clinical trial of okt3, a monoclonal anti-t-cell antibody, for prevention of kidney transplant rejection. 13 patients receiving a first cadaveric kidney transplant were randomly assigned to conventional treatment with azathioprine and high-dose steroids (7 patients) or to treatment with daily injection of okt3 alone (6 patients). the first okt3 injection resulted in a dramatic decrease in t3+, t4+, and t8+ cells, while patients simultaneously experienced fever, chills, and ... | 1986 | 3520988 |
| heredity and changes in hormones and metabolic rates with short-term training. | the purpose of this experiment was to assess the effects of a 22-day training program on resting metabolic rate (rmr), thermic effect of a meal (tem), and associated hormonal changes. six pairs of male monozygotic twins were submitted to a 22-day ergocycle exercise program designed to induce a deficit in energy balance of 4.2 mj per day. rmr and tem after a 4.2-mj meal challenge were measured before and after training. results indicated that rmr and tem did not change significantly, although a l ... | 1986 | 3521318 |
| endoluminal ultrasound in the assessment of local invasion in rectal cancer. | a new method for staging local invasion in rectal cancer has been assessed. thirty-eight patients with primary rectal cancers have been examined pre-operatively with endoluminal rectal ultrasound and results compared with histological analysis of resected specimens in 33 cases. three patients who also underwent laparotomy but not resection had good evidence of both stage t3 (1) and t4 (2) disease. the coefficient of correlation between ultrasonic and histological staging was 0.93 (p less than 0. ... | 1986 | 3521785 |
| serial immunologic studies in recipients of hepatic allografts. | thirty recipients of hepatic allografts had serial immunologic evaluations. prior to transplant, patients had marked depression of lymphocyte subsets and impaired in vitro immunoglobulin synthesis, while phytohemagglutinin responsiveness was similar to that of controls. following transplantation and introduction of cyclosporine and low-dose steroid therapy, there was a significant decline in both t cell subsets, but only the t4 population remained significantly depressed throughout the entire st ... | 1986 | 3521975 |
| specific lysis of murine cells expressing hla molecules by allospecific human and murine h-2-restricted anti-hla t killer lymphocytes. | the lysis by human and murine anti-hla cytolytic t lymphocytes (ctl) of murine cells expressing class i hla molecule after gene transfection has been studied using two different murine cells: lmtk- and p815-htr-tk-. weak but significant hla-a11-specific lysis was found occasionally with human ctl on the hla-a11+ l cells. on the contrary, p815-a11 or p815-a2 cells were lysed strongly and specifically by hla-a11 or hla-a2-specific human ctl. the t8+t4- phenotype of the effector cells was confirmed ... | 1986 | 3522244 |
| characterization of mononuclear cell subsets in renal cellular interstitial infiltrates. | indirect immunoperoxidase analysis using monoclonal antibodies (mo ab) was performed in 33 renal biopsies with interstitial cellular infiltration obtained from non-transplanted patients. we reviewed four acute interstitial nephritis (in), three chronic in, four granulomatous in, four acute tubular necrosis, four vasculitis, seven primary glomerulonephritis and seven active lupus nephritis (ln). we used mo ab recognizing t and b cell markers [okt3, okt8, t4, b1, iot14 (il2 receptor)], hla-dr rela ... | 1986 | 3523002 |
| immunohistochemical analysis of the cellular infiltrate in multiple sclerosis lesions. | immunohistochemical staining of 16 brains post mortem from patients with progressive multiple sclerosis and of two biopsy specimens from patients with acute demyelinating disease was performed using a panel of monoclonal antibodies reactive with t cells and t-cell subsets, b cells, and ia (hla-dr) antigens. lymphocytic perivascular cuffs were most prominent at the edge of active plaques and were occasionally seen in areas with no evidence of demyelination or macrophage infiltration. perivascular ... | 1986 | 3524414 |
| [a comparative diagnostic study of staging in renal cell carcinoma]. | a comparative diagnostic study was carried out on 56 patients with pathologically proven renal cell carcinoma which had been staged by ct, us, angiography (ag) and lymphography (lg) between june, 1980, and may, 1985. each study was performed within two weeks. the confirmation of the tumor extent was established by surgery and microscopic examination in all patients except three, in whom the extent of the tumor was determined at autopsy. the staging was performed according to the "general rules f ... | 1986 | 3524469 |
| hormonal control of a low km (type ii) iodothyronine 5'-deiodinase in cultured nb41a3 mouse neuroblastoma cells. | the central nervous system manifests complex homeostatic mechanisms for the maintenance of thyroid hormone economy. the present studies used the nb41a3 mouse neuroblastoma cell line as a model system to study the hormonal regulation of the enzymatic conversion of t4 to t3 in neural tissue. nb41a3 cells manifested a thiol-dependent 6-n-propyl-2-thiouracil-insensitive iodothyronine 5'-deiodinase (i5'd) with a km for t4 of approximately 10 nm. i5'd activity was increased 2- to 4-fold in cells grown ... | 1986 | 3525124 |
| inositol and hepatic lipidosis. ii. effect of inositol supplementation and time from parturition on serum insulin, thyroxine and triiodothyronine and their relationship to serum and liver lipids in dairy cows. | percutaneous liver biopsies and blood samples were obtained from 80 dairy cows in nine michigan herds over the peripartum period. thirty-nine cows were fed 17 g of supplemental inositol and 41 were fed a placebo. liver biopsies were assayed for total myoinositol and triglyceride (tg) concentrations. blood samples were assayed for serum dextran precipitable cholesterol, nonesterified fatty acids (nefa), insulin, thyroxine (t4), free (ft4), triiodothyronine (t3) and free t3 (ft3) concentrations. s ... | 1986 | 3525488 |
| the dna scanning mechanism of t4 endonuclease v. effect of nacl concentration on processive nicking activity. | t4 endonuclease v is a pyrimidine dimer-specific endonuclease which generates incisions in dna at the sites of pyrimidine dimers by a processive reaction mechanism. a model is presented in which the degree of processivity is directly related to the efficacy of the one-dimensional diffusion of endonuclease v on dna by which the enzyme locates pyrimidine dimers. the modulation of the processive nicking activity of t4 endonuclease v on superhelical covalently closed circular dna (form i) which cont ... | 1986 | 3525529 |