Publications

[use of flutamide in the treatment of carcinoma of the prostate stages c and d (t3-t4, nx-n0, m0-m1 categories)].		1986	3738681
free thyroxine estimation for the screening of hyper- and hypothyroidism in an adult population.	serum free thyroxine (ft4), total t4 (t4) and total t3 (t3) were determined by radioimmunoassay in 1,114 adults during a periodic health evaluation to detect unsuspected thyroid dysfunction. as a result, 3 patients with hyperthyroidism, 4 with hypothyroidism, 3 taking thyroid medication, 4 with chronic thyroiditis, 1 with simple goiter and 3 women under estrogen administration were found. free t4 values were within the normal limits in the present four subjects taking estrogens, but all of them ...	1986	3738907
respiratory effects of high thoracic epidural anaesthesia.	the respiratory effects of high thoracic epidural anaesthesia (tea) were studied in nine healthy volunteers by means of spirometry, nitrogen single-breath test and flow-volume loop. after the baseline measurements an epidural catheter was inserted at t4 level, and 5 ml of 0.5% bupivacaine were injected. this volume led to sensory block within dermatomes t1-t5. total lung capacity, vital capacity and inspiratory capacity decreased slightly but significantly during tea. expiratory reserve volume w ...	1986	3739578
serum thyroid hormones and tissue 5'-monodeiodinase activity in acutely thyroidectomized newborn lambs.	after either total thyroidectomy or sham operation in full-term fetal sheep, fetuses were delivered and serial blood samples were obtained for measurements of thyroxine (t4), triiodothyronine (t3), and catecholamines. despite comparable serum t4 values (t4 means +/- se, sham 7.1 +/- 0.6 micrograms/dl, thyroidectomized 6.8 +/- 0.7 micrograms/dl at 60 min after birth), serum t3 values were lower in the thyroidectomized animals (t3 means +/- se, thyroidectomized 39 +/- 4.8 ng/dl, sham 153 +/- 20.1 ...	1986	3740254
effects of caloric deprivation on thyroid hormone tissue uptake and generation of low-t3 syndrome.	changes in thyroid hormone metabolism in the low-3,5,3'-triiodothyronine (t3) syndrome cannot be fully explained in all conditions by a decrease in 5'-deiodinase activity. recent observations showed that in rat hepatocytes iodothyronines are taken up by an active transport mechanism. to investigate whether regulation, i.e., inhibition of active transmembraneous transport for iodothyronines in humans may contribute to the generation of the low-t3 syndrome, tracer thyroxine (t4) and t3 kinetic stu ...	1986	3740255
autonomic dysreflexia in a paraplegic man with catecholamine-secreting neuroblastoma.	autonomic dysreflexia and catecholamine secreting tumor, each of which causes paroxysmal hypertension, coexisted in a young man. two years after neuroblastoma was diagnosed, he developed t4 incomplete paraplegia due to metastases to the spine at t5 and l3 levels. shortly after the onset of paraplegia, paroxysmal hypertension developed. the hypertension was controlled adequately by good bowel and bladder management and oral clonidine. the paroxysmal hypertension is believed to have resulted from ...	1986	3741083
[effect of iodine, copper and zinc supplements to rations with a high quota of rapeseed extract meal on the growth and thyroid function of fattening swine. 3. the effect on weight and the histomorphometric findings in the thyroid and on the serum t3 and t4 concentrations].	studies were made with a total of 272 fattening pigs. iodine deficiency both in rations of soya bean meal and rape seed meal increased the weight of the thyroid gland and the height of the epithelium cells and significantly reduced the t4 and t3 level in the serum. iodine supplements to the rape seed meal rations distinctly diminished the goitrogenic effect, did not, however, cancel it out. stage of goiter and depression of consumption were--different shown by the t3 and t4 level in the serum. h ...	1986	3741130
the effect of tamoxifen therapy on thyroid function tests.	serum tri-iodothyronine (t3), thyroxine (t4) and thyroid-stimulating hormone (tsh) concentrations were measured in 50 postmenopausal patients with breast cancer receiving tamoxifen therapy. ten patients (20%) showed an elevation of serum total t4 concentrations and two of these patients showed additional elevation of serum total t3 levels. one patient had a persistently raised free t4 concentration and was considered to be thyrotoxic. serum thyroxine-binding globulin (tbg) was estimated in six p ...	1986	3742461
the effects of exogenous melatonin on endocrine function in man.	at two different times of year (spring and autumn) an oral preparation of the pineal neurohormone melatonin, or placebo, was administered to 12 healthy volunteers (10 men and two women in spring: the same group minus one man in autumn) daily at 1700 h for 1 month (spring), or 3 weeks (autumn) using a double-blind cross-over protocol. the daily dose was 2 mg melatonin in 5 ml corn-oil, and placebo consisted of the vehicle only. in spring the anterior pituitary hormones lh, prl, gh together with t ...	1986	3742833
cell-mediated immunity in anorexia nervosa.	twelve patients with anorexia nervosa were studied for cell-mediated immunity in terms of delayed hypersensitivity reactions to recall antigens, lymphocyte transformation responses to t-cell mitogens, and numbers of circulating leucocytes and t-cell subpopulations. compared to controls, all patients had reduced cutaneous reactions and four were anergic. there was a mild leucopenia in patients and both t4+ and t3+ numbers were slightly reduced. mean peak transformation responses for patients were ...	1986	3742879
thyroid ectopia in a congenital hypothyroid infant.	an infant girl was referred for thyroid screening. the t4 value was 5.0 micrograms/dl (normal 5.0-13.0 micrograms/dl), and the thyroid-stimulating hormone-radioimmunoassay value was greater than 60.0 microiu/ml (normal 0-10.0 microiu/ml). imaging failed to demonstrate thyroid tissue in the normal anatomic position in the neck. a focal collection of activity was seen, however, on a scan corresponding to the position of the base of the tongue. the finding is consistent with the diagnosis of a ling ...	1986	3742922
late hypothyroidism following subacute thyroiditis.	the authors followed up the incidence of permanent hypothyroidism in 35 patients with subacute thyroiditis (sat) in their past history. the investigations were made one to four years after the acute episode. ten of these presented repeated recurrences despite the therapy with glucocorticoids. the investigations included serum determination by ria of t4, t3, tsh and thyroglobulin as well as of circulating thyroid antibodies. permanent hypothyroidism subsequent to sat occurred in two patients. in ...	1986	3743646
influence of pharmacological doses of iopanoic acid and thyroxine 5- and 5'-monodeiodination in liver and kidney of the pig.	short-lasting (24-48 h) iop administration to growing pigs causes the inhibition of both 5- and 5'-thyroxine monodeiodination in the liver and the kidney. after administration of iop in vivo or into tissue homogenates, the inhibition of t4 to t3 conversion was stronger than that of t4 to rt3. the inhibitory effect was related to the thyroid hormones concentration in serum and to the age of animals.	1986	3743648
no monthly variations in serum levels of thyroid hormones in human males.	there have been very few reports on rhythmic variation in thyroid hormone concentrations with a periodicity of 4-6 weeks. in an attempt to evaluate whether such a rhythm is a general phenomenon we studied ten healthy male students with weekly blood samples over 12 weeks for t4, t3 and tsh. no rhythmicity could be demonstrated.	1986	3744295
amiodarone-induced thyroid dysfunction: diagnostic role of free serum thyroid hormone levels and pathogenetic significance of antithyroid antibodies.	forty-six patients treated with amiodarone hydrochloride for a mean period of 41 +/- 3.5 months, with an average daily dose of 240 +/- 57 mg/day, have been studied. thyroid function was assessed clinically and by laboratory tests, which included free-triiodothyronine (free-t3), free-thyroxine (free-t4) and thyrotropin. antimicrosomal and antithyroglobulin antibodies were also looked for. three patients (6.5%) were found to be hyperthyroid and two (4.3%) hypothyroid. of the 41 clinically euthyroi ...	1986	3744627
invasion of the mandible by squamous carcinomas of the oral cavity and oropharynx.	the radiologic and histologic features of mandibular invasion, and its clinical implications, are considered in a retrospective series of 111 patients with squamous carcinomas of the oral cavity and oropharynx treated by composite resection. eighty percent of the entire group had either recurrent or advanced (t3, t4) local disease, and 33 patients (30%) had histologic evidence of mandibular invasion by tumor. preoperative radiologic assessment was unreliable in cases in which infiltrating tumor ...	1986	3744855
rat liver iodothyronine monodeiodinase. evaluation of the iodothyronine ligand-binding site.	ligand binding characteristics of rat liver microsomal type i iodothyronine deiodinase were evaluated by measuring dose-response inhibition and apparent michaelis-menten or inhibitor constants of iodothyronine analogues to compete as substrates or inhibitors for the natural substrate l-thyroxine. these data show strong correlations with the binding requirements of hormone analogues to serum thyroxine-binding prealbumin since iodothyronine analogues with a negatively charged side chain, a negativ ...	1986	3745159
effects of iodide and thyroxine on iodine-deficient mouse thyroid: a morphological and functional study.	the effects of iodide and thyroxine (t4) on female mice fed a low iodine diet (lid) for 8 weeks were analysed by morphological, stereological and biochemical methods. iodide was given at a dose of 10 micrograms/day (hid) or 1 microgram/day (mid), either alone or together with daily injections of 1 microgram t4 for 8 or 40 days. with hid, the thyroid weight and the numbers of follicles and cells remained higher than in controls, although cell necrosis occurred. colloid volume increased and iodine ...	1986	3746158
control of sex steroid-binding protein (sbp) in the male little brown bat: relationship of plasma thyroxine levels to the induction of plasma sbp in immature males.	the seasonally reproductive male little brown bat (myotis lucifugus lucifugus) exhibits marked increases in plasma concentrations of sex steroid-binding protein (sbp) in the spring following arousal from hibernation. in this species an increase in sbp levels is induced prematurely in male bats aroused during the first half of hibernation and housed under long photoperiods; however, this rise is inhibited in bats housed under short photoperiods. in order to investigate the physiological role of t ...	1986	3746164
in-vivo studies of a human-thyrotrophin preparation.	the effects on thyroid function of a new preparation of human thyrotrophin (htsh) were studied in four subjects whose endogenous production of tsh had been suppressed by administration of thyroxine (t4). the htsh, prepared from human cadaveric pituitary glands and highly purified using a monoclonal antibody technique, was given as an intravenous bolus of 2 i.u. htsh. serum tsh levels rose rapidly to a maximum of about 150 mu./l and then declined exponentially with a half-life of 100 min. after i ...	1986	3746169
the analysis of expanded cells from patients with lymphoproliferative disorders of granular lymphocytes may help to clarify the nk cell differentiation.	surface phenotype and functional in vitro activities were studied in 2 cases of lymphoproliferative disorders of granular lymphocytes. cells from both patients presented the same, previously unreported, surface phenotype (i.e. t3+, t8+, t4-, hnk-1-, nk-15+, m1-), were unable to display either natural killer (nk) activity or suppressor function in a poke-weed-driven system, and showed a defective response to phytohemagglutinin (pha). on the basis of available schemes for nk cells ontogenesis, we ...	1986	3746877
isolation of bacteriophage t4 dna polymerase mutator mutants.	more than 20 new bacteriophage t4 dna polymerase mutants have been isolated by a procedure designed to select mutants with high spontaneous mutation rates. some of the mutants produce the highest mutation frequencies that have been observed in t4 thus far. the design of the selection procedure allows for the isolation of mutator mutants that preferentially induce certain types of replication errors, and some of the mutator mutants have mutational specificities different from wild-type. the new m ...	1986	3746907
[distant metastases from carcinoma of the oral cavity and pharynx].	distant metastases were detected clinically in 25 (14%) of 173 patients with squamous cell carcinoma of the oral cavity and pharynx admitted from 1975 to 1982. the probability of distant metastases was higher in patients with advanced tumor (stages iii and iv: 21%; t4: 27%; n3: 37%), lymphoepithelioma (57%) and in patients with a decreased lymphocyte count in the peripheral blood at presentation (less than 1300/microliter; 58%). the incidence of metastases in advanced cases was 45% in the oropha ...	1986	3747147
relationship of thyroid hormones and norepinephrine to the lateral hypothalamic syndrome.	the role of thyroid hormones and norepinephrine in the elevated thermogenesis seen following lateral hypothalamic (lh) lesions was investigated by measuring serum thyroid hormone levels and urinary norepinephrine excretion during the 24 hours following placement of lh lesions and again one month later when body weight had stabilized at a reduced level. during the first 24 hours following lh lesions, serum thyroxine (t4) and triiodothyronine (t3) were significantly depressed. by one month postles ...	1986	3747841
[effect of dietary supply of calcium on thyroid function in rats].	to determine if calcium had a goitrogenic effect on the thyroid function in rats, weanling rats were fed, for three weeks, a diet containing either 0.5 microgram or 0.04 microgram iodine per gram of diet, or an adequate (0.47%) or an excessive (2%) amount of calcium. with an adequate iodine diet, the calcium load did not induce an increase in the weight of the thyroid or a decrease in serum thyroid hormone concentration. however, the rats given a calcium load had a lighter body weight and a lowe ...	1986	3749601
response of total and 'free' thyroid hormones and diiodotyrosine to bovine tsh in subclinical hypothyroidism.	thirty-three patients with addison's disease were studied. twenty-two had idiopathic addison's disease; within this group, 14 patients had clinical or subclinical hypothyroidism, and 16 had increased titres of thyroid autoantibodies. five patients had tuberculous, and eight had unclassifiable addison's disease; only one patient in the latter group had evidence of thyroid autoimmunity. a stimulation test with 15 mu btsh/kg was performed in three patients with schmidt's syndrome (coexisting addiso ...	1986	3751463
thyroxine monodeiodination in normal human kidney tissue in vitro.	the present study deals with thyroxine monodeiodination in normal human kidney. to allow for comparison with previous reports, the present methods are similar to those used by others in rat tissue studies. the microsomal cell fraction of normal human kidney tissue was obtained by differential ultracentrifugation. the microsomes were incubated under various conditions and the deiodination products assayed with radioimmunoassay. a type i 5'-monodeiodinase was demonstrated, ph optimum around 6.5. c ...	1986	3751464
effects of amiodarone and desethylamiodarone on pituitary deiodinase activity and thyrotropin secretion in the rat.	the effect of acute administration of amiodarone, its major metabolite desethylamiodarone and iodine in an amount equal to that contained in amiodarone on serum thyroid hormone and thyrotropin (tsh) concentrations and hepatic and pituitary 5' deiodination of thyroxine (t4) in the euthyroid and hypothyroid rat was evaluated. amiodarone, desethylamiodarone and iodine all caused a decrease in serum t4 and triiodothyronine (t3) concentrations in euthyroid rats, while serum tsh concentrations and pit ...	1986	3752159
characteristics of thyroxine 5'-deiodinase activity in human liver.	the conversion of thyroxine (t4) to triiodothyronine (t3) was studied in homogenates and subcellular fractions of 10 human liver specimens obtained postmortem. preliminary studies indicated that t4 5'-deiodinase activity did not decline in rat liver kept at 5 degrees c for 6 and 24 hr after death. all human liver homogenates but one catalyzed t3 production, although the quantity of t3 produced varied greatly, from 8-fold in the absence of dithiothreitol (dtt) to 100-fold in its presence. the wid ...	1986	3752164
protection by thyroxine in nephrotoxic acute renal failure.	thyroxine (t4) protects against ischemic and nephrotoxic experimental acute renal failure (arf). this study examined functional, biochemical, and morphological markers of uranyl nitrate (un)-induced renal injury in the rat to determine the cellular site at which t4 exerts its protective effect. in experimental group unt4, 1-thyroxine (10 micrograms/100 g body wt) was given for 10 days prior to and for 4 days following a single subcutaneous injection of un (0.5 mg/kg body wt). group un received o ...	1986	3752254
chemical synthesis of octadeoxyribonucleotides containing sequence-specific 3'-deoxy-3'-chlorouridine residues and 2'-5' internucleotide linkages by the phosphite chloridite approach.	3'-deoxy-3'-chlorouridine has been chemically incorporated into two octanucleotides at various positions using in situ prepared phosphite chloridite intermediates. the behaviour of these modified selfcomplementary dna fragments containing 2'-5' internucleotide bonds in the neighbourhood of 3'-deoxy-3'-chlororibose in respect to chemical sequencing, polynucleotide kinase and t4 dna ligase has been studied.	1986	3753470
hypothalamic-pituitary function of children with acute lymphocytic leukemia after three forms of central nervous system prophylaxis. a retrospective study.	the hypothalamic-pituitary function of 93 children, who had received central nervous system (cns) prophylaxis as part of their therapy for acute lymphocytic leukemia (all), and who remained in continuous complete remission, was evaluated retrospectively. treatment regimens included--group i: 31 subjects, intrathecal methotrexate (it mtx); group ii: 31 subjects, it mtx plus 2400 rad cranial irradiation; and group iii: 31 subjects, it mtx and intravenous intermediate-dose methotrexate. serum thyro ...	1986	3753892
serum triiodothyronine to thyroxine ratio: a newly recognized predictor of the outcome of hyperthyroidism due to graves' disease.	patients with untreated hyperthyroidism due to graves' disease have a proportionally greater increase in the serum t3 than in the t4 concentration and, therefore, have an elevation of the serum t3 to t4 ratio. the aim of this study was to investigate the alterations of the serum t3 to t4 ratio in relation to the outcome of antithyroid drug therapy. of 47 patients with hyperthyroid graves' disease, 37 patients had a serum t3 to t4 ratio greater than 20 ng/micrograms before therapy (normal range, ...	1986	3754263
spontaneous regression of a monoclonal proliferation of large granular lymphocytes associated with reversal of anemia and neutropenia.	a 43-year-old male with a phenotypically homogeneous, expanded subset of t cells presented in 1981 with anemia and neutropenia. the surface antigen phenotype of 99% of the peripheral blood lymphocytes was t3+, t8+, t4-, and they were morphologically large granular lymphocytes (lgl). the same cells comprised 37% of the marrow nucleated cells. eight months after he presented, the peripheral blood t8+, lgl diminished spontaneously, and the anemia and neutropenia completely resolved. the patient rem ...	1986	3754473
the effect of iodide ingestion on the development of spontaneous lymphocytic thyroiditis in the diabetes-prone bb/w rat.	it has been suggested that the incidence of hashimoto's thyroiditis is increased in the presence of high iodide intake. the diabetes-prone bb/w rat develops spontaneous histological autoimmune lymphocytic thyroiditis (lt) without functional hypothyroidism between 60 and 120 days of age. studies were carried out to determine whether iodine administration to bb/w rats would affect the incidence and severity of lt and induce hypothyroidism. iodide (0.05% in water) or tap water (c) was administered ...	1986	3754509
sequence signals which may be required for efficient formation of mrna 3' termini.	it has been known for sometime that aataaa (proudfoot and brownlee, 1976) is often required for 3' mrna processing. more recently, mclauchlan et al. (1985) have shown the high incidence of ygtgttyy (y is a pyrimidine) downstream from the poly (a) addition site. our results fully support their findings. there have been indications that additional sequences are required either for transcription termination of mrna or for its cleavage/processing. here i present the results of detailed analysis of m ...	1986	3754635
lymphocyte subpopulation abnormalities in sickle cell anemia: a distinctive pattern from that of aids.	in this study, we evaluated lymphocyte subpopulations in 23 adults with sca. when compared to controls, sca patients had higher lymphocyte counts with normal numbers of t101+ cells (t-lymphocytes) and t4+ cells. t8+ cells were significantly increased in sca patients in comparison to controls (1684 +/- 243 vs 980 +/- 367, p less than .001). this increment was largely dependent on a t101-, t8+ cell population. the sca patients as a group had significantly decreased t4/t8 ratio (p less than .0001). ...	1986	3754689
profound atracurium induced neuromuscular blockade. a comparison of evoked reversal with edrophonium or neostigmine.	speed of reversal of profound atracurium induced neuromuscular blockade following edrophonium (0.5 or 1.0 mg/kg) or neostigmine (0.04 or 0.08 mg/kg) was measured using the train-of-four pattern of nerve stimulation. in all patients adequate clinical reversal was present when the ratio of the strength of the fourth to the first twitch (t4 ratio) was 0.5. both doses of edrophonium were associated with a significantly faster speed of reversal than the smaller dose of neostigmine (p less than 0.05 i ...	1986	3754698
primary malignant lymphoma of the anorectum in homosexual men.	two homosexual men presented with primary malignant lymphomas of the anorectum. each patient presented with a painful mass, diagnosed by biopsy and immunologic cell marker studies. one patient, who had a reversed t4/t8 cell ratio, developed a local relapse of the tumor 11 months after radiotherapy, and pneumocystis carinii pneumonia. the other patient, with a normal t4/t8 ratio, remained lymphoma-free and without evidence of opportunistic infection 25 months after combined chemotherapy and radio ...	1986	3754810
changes in plasma fibronectin levels in thyroid diseases.	the plasma levels of fibronectin (fn) have been measured in normal subjects and in patients with thyroid diseases. the mean plasma fn levels in 62 normal adults was 32.0 +/- 6.0 mg/dl, whereas it was elevated to 62.6 +/- 16.1 mg/dl (mean +/- sd) in 25 patients with hyperthyroidism and decreased to 19.2 +/- 8.0 mg/dl in 9 patients with hypothyroidism. the 9 patients with simple goiter have normal values of 29.1 +/- 8.0 mg/dl. with the administration of anti-thyroid drugs, plasma fn levels normali ...	1986	3755115
[preoperative preparation with plasmapheresis of patients with diffuse toxic goiter and intolerance to antithyroid drugs].	the authors analysed the results of therapy with plasmapheresis of 46 patients with diffuse toxic goiter showing intolerance to antithyroid therapy for the purpose of preoperative preparation. a therapeutic effect was achieved in 45 patients. plasmapheresis resulted in the reduction of a period of preoperative preparation, the concentration of t4 and jgg, iga and igm decreased and the tth level increased.	1986	3755531
effect of azimexon therapy on host defense parameters and disease-associated symptoms in the acquired immune deficiency syndrome (aids) and aids-related complex (arc).	azimexon, a 2-cyan-aziridinyl immune modulator, was given at a dose of 250 mg/m2/day for 10 days iv to 12 patients with aids and 16 with aids related complex (arc). a decrease in total number of aids related symptoms from 43 to 24 and in mean number from 2.6 to 1.5 was observed among arc patients (p less than .01). the most commonly improved symptoms were diarrhea, fatigue, and weight loss with the least frequently improved being lymphadenopathy. the following improvements in immune parameters w ...	1986	3755909
results of surgery following radical radiotherapy for invasive bladder cancer.	five hundred and ninety-one of 889 patients with t1 to t4 transitional cell carcinoma of the bladder had persistent or recurrent cancer after radical radiotherapy. durable local control was significantly poorer for patients with grade 1 or t4 cancer before radiotherapy. three hundred and twenty-two patients received additional surgical treatment: 211 were endoscopically managed and 111 had secondary cystectomy. the survival of patients with residual or recurrent cancer after radiotherapy was sig ...	1986	3756409
ontogenesis of nuclear t3 receptors in primary cultured astrocytes and neurons.	nuclear t3 receptor (ntr) have been characterized in separated cultures of neurons and astrocytes. scatchard analysis indicated the presence of a single class of high-affinity sites in both cell lines. the apparent equilibrium association constant ranged from 1.80 +/- 0.41 x 10(10) m-1 to 3.27 +/- 0.74 +/- 10(10) m-1 in neurons and from 1.01 +/- 0.09 to 1.80 +/- 0.73 x 10(10) m-1 in astrocytes depending on the time in culture. in neurons, the maximal binding capacity (mbc) increased from 0.049 + ...	1986	3756504
susceptibility in urinary bladder cancer: acetyltransferase phenotypes and related risk factors.	the excess proportion of slow acetylator phenotypes has previously been found among bladder tumour patients. among 228 bladder tumour patients in the present study, the slow acetylator phenotype was significantly associated to the unskilled worker and t3-t4 tumour, and to a certain extent to tobacco smoking. it is suggested that slow acetylator subjects are more susceptible to bladder tumour development when exposed to arylamines.	1986	3756846
multiple self-splicing introns in bacteriophage t4: evidence from autocatalytic gtp labeling of rna in vitro.	rna from t4-infected cells yielded multiple end-labeled species when incubated with alpha-32p-gtp under self-splicing conditions. one of these corresponds to the previously identified intron from the td gene of t4, while others appear to represent additional group i introns in t4. two loci distinct from the td gene were found to hybridize to a mixed alpha-32p-gtp-labeled t4 rna probe. these mapped in or near the unlinked genes nrdb and nrdc. a fragment from the nrdb region that contains the intr ...	1986	3757035
natural evolution of thyrotropin and thyroid hormones concentrations in hypothyroid newborns with thyroid agenesis or ectopy during the first month of life.	thyroid hormones and thyrotropin concentrations obtained by different screening programs at different times after birth were pooled to study their natural evolution in congenitally hypothyroid newborns with thyroid ectopy or agenesis. in filter paper blood spots, the t4 concentrations were higher in the group with thyroid ectopy whereas it was the opposite for the tsh values. serum t4 decreased progressively in cases of thyroid agenesis. in ectopy, serum t4 remained stable after 2 weeks whereas ...	1986	3757325
changes in the hypothalamic-pituitary-thyroid axis during acute starvation in non-obese patients.	we investigated changes in the hypothalamic-pituitary-thyroid axis before, during, and after fasting in twenty-one non-obese euthyroid patients with psychosomatic diseases. blood samples for free t3 (ft3), t3, free t4 (ft4), t4, reverse t3 (rt3), and tsh were obtained from all patients before and on the 5th day of fasting, and in 11 of the same individuals on the 5th day of refeeding. serum tsh and t3 responses to trh were also evaluated in 10 patients before and on the 5th day of fasting. durin ...	1986	3757921
an improved and simplified method for the detection of thyroid hormone autoantibodies (thaa) in serum.	we have developed and evaluated a new and simplified method for the detection of thyroid hormone autoantibodies (thaa) in serum. the method includes acidification of serum followed by adsorption of liberated thyroid hormones onto dextran-coated charcoal and then alkalinisation of the serum in assay buffer prior to performing a binding study. using our method, specific binding of 125i-t4 to serum thaa in two patients with hashimoto's thyroiditis was almost the same regardless of whether or not th ...	1986	3757925
the place of brachytherapy in the treatment of carcinoma of the tonsil with lingual extension.	one hundred forty-four of 170 patients (85%) were seen with cancer of the tonsil and received radical irradiation between 1959 and 1980. a 39% crude 3-year disease-free survival rate and a 51% locoregional control rate were observed. locoregional relapse related to t stage was 6, 43, 58, and 64% for t1, t2, t3, and t4, respectively. fifty-four of 144 patients (37%) showed tongue extension, 20% in t1-t2 stages and 50% in t3-t4 stages. local relapse was 64% and the 3-year disease-free survival rat ...	1986	3759531
appearance of mania in drug-resistant bipolar depressed patients after treatment with l-triiodothyronine.	l-triiodothyronine (t3) has been reported to potentiate the antidepressant effects of tricyclic antidepressants (tcas) in patients who do not respond to these drugs, while thyroxine (t4) has been used to treat rapid-cycling bipolar disorder patients. the development of mania in antidepressant-resistant bipolar depressed patients after t3 was added to their antidepressant treatment is reported. it is speculated that thyroid hormone-catecholamine receptor interaction might underlie these t3-associ ...	1986	3759918
in-vitro conversion of thyroxine to tri-iodothyronine by chicken hepatic 5'-deiodinase: kinetic studies.	in-vitro studies with chicken liver homogenates demonstrate that the conversion of thyroxine (t4) to tri-iodothyronine (t3) is dependent upon tissue concentration, time of incubation, ph, temperature, the presence of dithiothreitol (dtt) and the concentration of substrate (t4), and is heat-labile. the generation of t3 is inhibited by iopanoic acid and 6-n-propyl-2-thiouracil. the kinetics of conversion of t4 to t3, determined by lineweaver-burke analysis, indicated an apparent michaelis-menten c ...	1986	3760742
performance of solid-state 48 detector gamma counter using solid phase total thyroxin and free thyroxin assays.	we evaluated the performance of an automated 48 detector kineticount gamma counter using solid phase reagents for total thyroxin (tt4) and free thyroxin (ft4). interassay coefficients of variations (cv) for both total t4 and free t4 ranged from 5.4% to 13%. between-method correlation coefficients were 0.9798 for tt4 and 0.8844 for ft4. detection limits for tt4 and ft4 were 3 micrograms/l and 3 ng/l, respectively. tt4 was linear up to 200 micrograms/l. the recovery of tt4 was between 99 to 101%. ...	1986	3760986
conversion of thyroxine into tri-iodothyronine in zinc deficient rat liver.	hepatic thyroxine (t4) to tri-iodothyronine (t3) conversion was measured in six animal groups: group a was fed a severe zinc-deficient diet (1.98 ppm) for 5 weeks; group b was a pair-fed control group for group a; group c was fed a less severe zinc-deficient diet (6.10 ppm) for 5 weeks; group d was a pair-fed control group for group c; group e was fed a zinc-supplemented control diet (90.4 ppm) for 5 weeks; and group f was first fed the severe zinc-deficient diet for 5 weeks and then placed on t ...	1986	3761111
effects of chlorine dioxide on thyroid function in the african green monkey and the rat.	in a previous study from this laboratory, chlorine dioxide (clo2) treated drinking water depressed thyroxine (t4) levels in the african green monkey. the present study again demonstrated a decrease in t4 levels in the same species after 4 wk of oral exposure. however, after 8 wk of treatment t4 levels rebounded to above pretreatment levels, coinciding with an increase in thyroid radioiodide uptake. this t4 rebound phenomenon and increased iodide uptake may be due to a compensatory endocrinologic ...	1986	3761383
[the scissors phenomenon in the reaction of hormonal systems to the physical load test in ischemic heart disease patients].	a difference between baseline and final plasma hormonal (insulin, cortisol, somatotrophic hormone, triiodothyronine (t3) and thyronine (t4) levels, related to the pattern of response to physical stress, was demonstrated in 67 coronary patients with angiographically verified diagnosis. post-exercise increase in these hormones was associated with their lowered baseline and elevated final blood levels, as compared to those patients who showed a decrease in hormonal levels in response to exercise, w ...	1986	3761806
thyroid function in critically ill infants with infections.	to investigate thyroid function in critical illness in infancy, serum thyroxine (t4), triiodothyronine (t3), reverse triiodothyronine (rt3) and thyroid-stimulating hormone (tsh) concentrations were measured in serum in 13 such patients. t4 and tsh values were found to be comparable to control values, while t3 values were significantly low and rt3 values were high. when the patients were evaluated according to prognosis, significantly lower initial and subsequent t4 values were found in the fatal ...	1986	3763416
the effects of d-phenylalanine and its derivatives on enkephalin degradation in vitro: relation to analgesia and attenuation of the morphine withdrawal syndrome.	the effect of d-phenylalanine (d-phe), putative carboxypeptidase a inhibitor and its four derivatives (t1-t4) on analgesia, development of tolerance and physical dependence to morphine, and on degradation of both exogenous and endogenous enkephalins was investigated. systemic administration of either d-phe or its derivatives produced naloxone-reversible analgesia in the hot-plate test in mice. naloxone-precipitated morphine withdrawal syndrome was attenuated in mice after systemic subacute admin ...	1986	3763485
[reflexometry: an early prognostic test for assessing postoperative hypothyroidism].	the serum content of thyroxin (t4) and the duration of achilles reflex was studied in 23 patients with toxic goiter 1-3 months after subtotal thyroidectomy. concentration of t4 assessed in the serum revealed 9 euthyroid subjects and 14 patients with hypothyrosis. but as the duration of achilles tendon reflex was within the norm in all 14 hypothyrosis patients reflexometry technique turned to be inconsistent in distinguishing between hypo- and euthyroidism. however the high prognostic value of th ...	1986	3763567
[preoperative tumor staging in rectal cancer--a prospective study].	a precise preoperative tumor staging is the prerequisite of distinct treatment planning and subtle operative regimen. diagnostic contribution of the various examination techniques especially of imaging modalities were determined in a prospective study and correlated to intra- and postoperative findings. the tnm classification of malignant tumors of the uicc, both in its present valid and the modified form to be introduced in january 1987, formed the basis of our clinical diagnostic staging. diag ...	1986	3763846
effects of frequent and sustained plateletapheresis on peripheral blood mononuclear cell populations and lymphocyte functions of normal volunteer donors.	per procedure, plateletapheresis may remove 2 x 10(9) to 3 x 10(9) white cells from the peripheral blood of a normal donor. to investigate the effects of frequent and sustained plateletapheresis, multiple peripheral blood tests were performed on 25 volunteer donors undergoing plateletapheresis an average of 72 times over periods of up to 8 years, and results were compared with 25 age- and sex-matched controls who had not undergone apheresis. in donors, significant decreases were observed in: 1) ...	1986	3765039
relationships between thyroid hormone and catecholamines in experimental trauma.	the relationship between thyroid hormones (t4 and t3) and the catecholamines (adrenaline and noradrenaline) was studied as regards the possible value of administered t3 in the treatment of severely injured patients. preparative surgery was performed on 36 pigs, 26 of which were then submitted to a standardized high-energy missile trauma to a hind leg. in 14 of the latter group, infusion of t3 was begun immediately before the trauma (n = 6), or after a delay of 8 h (n = 4) or 24 h (n = 4). a corr ...	1986	3766027
variation in serum levels of t3, t4, ft4 and tsh during thyroxine replacement therapy.	serum levels of t3, t4, ft4 and tsh were determined in 9 euthyroid patients during 6 h after intake of morning thyroxine replacement medication. four hypothyroid patients were studied in the same way after the intake of 50 micrograms thyroxine. in the euthyroid patients the ft4 level increased by 31%, while the increase of t4 was 16% and the increase of t3 11%. in the hypothyroid patients levels of t3, t4 and ft4 did not change after ingestion of 50 micrograms thyroxine, while the tsh level was ...	1986	3766048
conversion of thyroxine to 3,5,3'-triiodothyronine in several rat tissues in vivo: the effect of hypothyroidism.	the local conversion of thyroxine (t4) to 3,5,3'-triiodothyronine (t3) has been recognized as a source of t3 at various sites in euthyroid rats. the present study was designed to evaluate the effect of hypothyroidism on the source and quantity of t3 at several of these sites (liver, cerebral cortex (cx), thymus, testis, brown adipose tissue). for this purpose intact euthyroid rats and radiothyroidectomized (rtx) rats received a continuous iv infusion of [125i]t4 and [131i]t3 until isotopic equil ...	1986	3766050
intestinal phosphate and calcium absorption: joint regulation by thyroid hormones and 1,25-dihydroxyvitamin d3.	thyroxine (t4) and triiodothyronine (t3) activate na+-dependent inorganic phosphate (pi) transport in organ-cultured embryonic chick small intestine. induction of transport activity requires intact protein synthesis and can be expressed in enterocytes with varying degrees of differentiation. t3 and t4 exert their effect independent of 1,25-dihydroxyvitamin d3 (1,25(oh)2d3), which is shown to stimulate pi uptake only in the final stage of embryonic differentiation. at this time point, a potentiat ...	1986	3766052
a prospective critical evaluation of in vitro thyroid function tests.	a number of 2 325 serum samples from a population of in- and outpatients were collected during a six-month period in order to evaluate the usefulness of various thyroid function tests in the clinical laboratory routine. the samples were analysed with the following thyroid function tests: total triiodothyronine (t3) (tt3), total thyroxine (t4) (tt4), free t3 index (ft3i), free t4 index (ft4i) and thyrotropin (tsh). one to two years after the primary evaluation, a follow-up was performed and the f ...	1986	3766208
thyroid function tests in elderly finnish men.	serum total thyroxine (t4), triiodothyronine (t3) and thyrotrophin (tsh) levels were determined among 65-84-year-old finnish men living either in eastern finland (n = 309) or in southwestern finland (n = 389). the mean value for serum total t4 was 112.2 +/- 23.0 nmol/l in eastern finland and 111.3 +/- 21.9 nmol/l in southwestern finland. the mean value for serum t3 was 1.76 +/- 0.30 nmol/l in eastern finland and 1.75 +/- 0.46 nmol/l in southwestern finland. serum tsh values showed the mean for m ...	1986	3766209
[clinical analysis of high grade bladder cancer].	the prognosis and other clinical manifestation of 128 patients with high grade bladder tumor were analyzed. thirty two percent of the total cases of bladder cancer were high grade bladder cancer and 83% of their tumors were invasive tumor at stage t2 and worse. urinary cytologies were positive in 88% of these patients. the 5-year survival rate in these patients was 32% and those in t1, t2 t3 and t4 cases were 64.2%, 55.6%, 22.7% and 8.0% respectively. the patients treated with radical (total) cy ...	1986	3766339
thyroid function tests in patients with familial dysalbuminaemic hyperthyroxinaemia (fdh).	two patients with familial dysalbuminaemic hyperthyroxinaemia (fdh) are described in whom the albumin variant resulted in raised total t4 levels, and artefactually raised free t4 using a 'single-step' technique employing an analogue of t4 as tracer. the first patient was clinically euthyroid and presented with relapse of schizophrenia and abnormal thyroid function tests (total t4 336 nmol/l, total t3 4.2 nmol/l, tsh 1.8 mu/l, free t4 73 pmol/l). these results led to diagnostic confusion and the ...	1986	3767254
preparation of (t3 + t4)-free pooled plasma.		1986	3767280
[use of enzymun-t4 and enzymun-tbk tests to determine thyroid function in cattle].		1986	3767576
synthesis and properties of oligodeoxyribonucleotides containing an ethylated internucleotide phosphate.	internucleotide phosphotriesters comprise an important class of dna lesions produced by carcinogenic alkylating agents. to avoid confusion resulting from the presence of other dna lesions, synthetically prepared oligonucleotides containing ethylated internucleotide phosphates as the sole form of damage were employed to investigate several chemical and biochemical properties of dna alkyl phosphotriesters. a total of four oligonucleotides were synthesised for this study, the dimers tp(et)t and ptp ...	1986	3768334
hypothalamic neuroendocrine correlates of cutaneous burn injury in the rat: i. scanning electron microscopy.	rats were given a standard scald burn on 60% of the body surface or only a sham burn and were sacrificed at intervals from 6 hr to 14 days later. serum thyroxine (t4), free thyroxine index (ft4i) and triiodothyronine (t3) were depressed compared to values in respective shams as early as 6 hr post-burn. t4 and ft4i were less depressed on post-burn days (pbd) 2-3 than on pbd 1 and then exhibited a further fall. t3 remained depressed through pbd 14. pineal melatonin content was elevated at 6 hr and ...	1986	3768739
the wavelength dependence of u.v.-induced pyrimidine dimer formation, cell killing and mutation induction in human diploid skin fibroblasts.	in this study, we determined the wavelength dependence of u.v.-induced pyrimidine dimer formation, cell killing and mutation induction in human diploid skin fibroblasts. pyrimidine dimers were quantified using the t4 endonuclease v assay, cell killing was measured as loss of colony forming ability and mutation induction was detected at the hprt locus. u.v. irradiation was performed with monochromatic light of four different wavelengths (254, 297, 302 and 365 nm) and with polychromatic light of a ...	1986	3769130
t3/t4 ratio in cimetidine treatment.		1986	3769713
identification and characterization of l-triiodothyronine receptors in cells of glial and neuronal origin.	high affinity-low capacity binding sites for thyroid hormone have been identified in the nuclei of glial (c6) and neuronal (neuro 2a) cultured cells. equilibrium dissociation constants, determined by scatchard analysis, were very similar in both types of cells (0.2-0.3 nm). the relative affinity of hormonal analogs was also similar: the affinity for t3 was lower than for triiodothyroacetic acid and higher than for t4 or tetraiodothyroacetic acid. the sedimentation coefficients obtained by gradie ...	1986	3769867
the role of 3,3',5'-triiodothyronine in the regulation of type ii iodothyronine 5'-deiodinase in the rat cerebral cortex.	type ii iodothyronine 5'-deiodinase (5'd-ii) activity is the source of 75-80% of the cerebral cortex t3 content in euthyroid rats. the activity of this enzyme is increased in hypothyroidism and can be quickly suppressed by t4 and rt3 by mechanisms involving neither protein synthesis nor nuclear t3 receptors. we have examined the possibility that endogenous cerebrocortical rt3 levels play a physiological role in the regulation of this enzyme. thyroidectomized rats were injected with graded doses ...	1986	3769868
ontogenesis of growth hormone (gh)-responsive hepatic gene products: lack of correlation with hepatic gh receptor content.	the ontogenesis of hepatic gh receptor content is postulated as a major determinant of expression of hepatic gh-responsive gene products, since the ontogeny of the receptors and the responsive gene products, somatomedin-c, somatomedin-binding protein, and gh-responsive acidic protein, have similar qualitatively ontogenetic patterns. two-dimensional gel electrophoresis of in vitro synthesized [35s]methionine-labeled proteins formed in the cell-free mrna-dependent rabbit reticulocyte lysate system ...	1986	3769871
triiodothyronine (t3)-induced down-regulation of the nuclear t3 receptor in mouse preadipocyte cell lines.	as previously reported, preadipocytes cloned from the epididymal fat of lean or genetically obese mice (hgfu and ob 17, respectively) contain the nuclear t3 receptor. the number of receptor sites was similar in confluent cells of both lines and approximately doubled during adipocyte differentiation. t3 added to the culture medium increased triacylglycerol synthesis. t3 also increased fatty acid synthase specific activity, relative synthesis rate, and relative mrna content (1.5- to 2.5-fold). opt ...	1986	3769873
relationship between circulating thyroid hormones and humoral immunity in immature male chickens.	the objective of this experiment was to examine the relationship between levels of circulating t3, t4, and humoral immunity in immature male chickens. three week old single comb white leghorn male chicks were used as the experimental animals. in order to produce a wide range of circulating thyroid hormone concentrations, birds were divided into groups and received one of nine treatments including surgical thyroidectomy; 0.1% propylthiouracil (ptu) in the feed; 1 ppm t3 and 10 ppm t4 in the feed. ...	1986	3770270
annual cycle of plasma thyroid hormone levels in the toad, bufo japonicus.	plasma thyroxine (t4) of the toad, bufo japonicus formosus, was monitored monthly from march, 1981 to february, 1982. toads were captured at sayama, saitama prefecture, and individual blood samples were collected from the heart soon after capture in the field. the plasma t4 levels were minimal in november for males (0.096 ng/ml) and in december for females (0.038 ng/ml). levels increased during the resting stage in winter and reached a peak in march, about 1 week before the breeding migration, i ...	1986	3770432
the effects of sodium ipodate (oragrafin) on thyroid function in rainbow trout, salmo gairdneri.	immature rainbow trout held at 12 +/- 1 degree were injected intraperitoneally with a fine saline suspension of sodium ipodate (5 mg/100 g body wt) every 3 days. plasma 3,5,3'-triiodo-l-thyronine (t3) fell to 40% of control levels by day 1 and remained at about this level for the duration of the study (22 days). plasma l-thyroxine (t4) level was not altered on day 1 but was lowered to 50% of control values by days 7 and 22. immersion of trout in t4 (2 micrograms/100 ml water) elevated plasma t4 ...	1986	3770448
humoral immune responses to t cell tropic retrovirus simian t lymphotropic virus type iii in monkeys with experimentally induced acquired immune deficiency-like syndrome.	the t cell tropic retrovirus of macaque monkeys simian t lymphotropic virus type iii (stlv-iii) has morphologic, growth, and antigenic properties indicating that it is related to human t cell lymphotropic virus type iii/lymphadenopathy-associated virus (htlv-iii/lav), the etiologic agent of the acquired immune deficiency syndrome (aids) of humans. stlv-iii has recently been shown to induce an aids-like disease in macaque monkeys. in this study the humoral immune responses of six experimentally i ...	1986	3771794
[studies on the relationship between reproductive phenomena and thyroid function--dynamics of the concentrations of thyroid hormones in pregnancy, parturition and puerperium].	thyroid function during pregnancy, parturition and puerperium was studied by measuring the concentration of free thyroxine (f-t4), free triiodothyronine (f-t3) and reverse triiodothyronine (r-t3) in maternal and cord sera, and simultaneously metabolism of thyroid hormones in chorionic tissues was examined. results obtained were as follows: in cases of normal pregnancy, maternal f-t4 and f-t3 levels tend to decrease during the course of pregnancy toward parturition. pathological pregnancies such ...	1986	3772204
stimulatory effects of growth hormone and thyroxine on the concentration of gangliosides in the snell dwarf cerebrum.	the concentration of gangliosides in the snell dwarf mouse cerebrum was monitored from postnatal day 5 to day 40. in the dwarf cerebrum, the concentration of total gangliosides increased up to postnatal day 20 and then stopped, whereas in the control cerebrum, it continued to increase up to postnatal day 40. at postnatal day 40, the ganglioside level in the dwarf cerebrum was 70% of that in the control cerebrum. among the ganglioside species, the concentrations of gm4, gm2, gm1, gd1a, gd3, gd1b, ...	1986	3772377
[hypophyseal-thyroid system in chronic alcoholism].	the levels of the thyroid hormones in the peripheral blood of patients with stage i and ii chronic alcoholism increased moderately at the expense of ejection activation in the blood but not hyperproduction of the thyroid and correlated with a decrease in the secretion of corticosteroids and an increase in the acth level. disproportion of the t3 and t4 levels was in direct relation to a degree of alcoholic liver affection. in a long-term period following antialcoholic treatment hormonal function ...	1986	3773661
action of some pesticides on t4 to t3 conversion in cultured kidney and liver cells in the presence or absence of cysteine.	the influence of aminotriazole (ata), atrazine (atz), prometryne (p) and atc on t4 to t3 conversion in kidney and liver cell cultures in the presence or absence of cysteine was studied. the experiment was carried out on cell cultures (rat renal cells and human embryo cells) and organotypic cultures (rat liver cells), treated with the above mentioned pesticides in 2 doses each, in the presence of 1 microgram and 10 micrograms t4 in the culture medium. this experiment was reproduced in the same co ...	1986	3775222
[misuse of thyrostatic drugs in cattle: determination of thyroxine (t4) concentration and thyroid weight and detection of methylthiouracil (mtu)].		1986	3775350
diminished hepatic triiodothyronine production in gunn rats.	the liver is a major site of conversion of thyroxine (t4) to the more active thyroid hormone 3,5,3'-triiodothyronine (t3). hepatic t4 to t3 conversion is altered by a variety of pathological processes and pharmacological agents. we studied t4 to t3 conversion in glucuronyl transferase deficient homozygous gunn rats because they have a hepatic enzyme abnormality which leads to hyperbilirubinaemia, and also because they have been reported to have alterations in thyroid hormone metabolism. an in vi ...	1986	3776437
effects of betamethasone on serum iodothyronines and thyroid hormone-binding proteins in graves' disease.	the influence of betamethasone on iodothyronines and their binding proteins in serum was studied in 18 patients with graves' disease. betamethasone, 6 mg daily, was given orally for 5 days. serum triiodothyronine (t3) concentration decreased, reverse triiodothyronine increased, while thyroxine (t4) and thyroid-stimulating hormone were unchanged. thyroxine-binding globulin (tbg) decreased and thyroxine-binding prealbumin increased. in accordance with the decrease in tbg, the in vitro t3 uptake in ...	1986	3776699
amiodarone, thyroid hormone indexes, and altered thyroid function: long-term serial effects in patients with cardiac arrhythmias.	amiodarone, a drug that has electrophysiologic actions resembling those of hypothyroidism, increases serum levels of t4 and reverse t3 (rt3) and decreases t3. the drug's long-term effects on thyroid function are poorly defined. serial thyroid hormone indexes in 76 patients given amiodarone for 6 to 32 months (mean +/- standard deviation 16 +/- 7) for arrhythmias were determined serially. over this period, 68 patients (89%) remained euthyroid; hypothyroidism developed in 6 (8%) and hyperthyroidis ...	1986	3776855
serum tsh concentration as an aid to monitoring compliance with thyroid hormone therapy in hypothyroidism.	to monitor the compliance of patients taking levothyroxine as replacement therapy for primary hypothyroidism, the authors measured serum thyroid-stimulating hormone (tsh), free thyroxine index (ft4i), and free triiodothyronine index (ft3i) in patients attending the endocrine clinic. during a 6-month period, there were 159 visits by 132 patients with treated hypothyroidism. five of the 132 patients had tsh levels greater than 12 microu/ml (normal, 0.3-5.7 microu/ml), with ft3i greater than 85 (no ...	1986	3777010
the effect of methimazole on the development of spontaneous lymphocytic thyroiditis in the diabetes-prone bb/w rat.	methimazole (mmi) administration decreases the incidence and intensity of experimentally induced lymphocytic thyroiditis (lt) in the female august rat and male a/j mouse. spontaneous lt frequently occurs in the insulin-dependent type-i diabetic (dm) bb/w rat. experiments were carried out to determine whether mmi administration to bb/w rats from 30 to 120 days of age would affect the incidence and intensity of spontaneous lt and the incidence of spontaneous dm. a total of 870 ng mmi/gm bw or sali ...	1986	3777011
[acquired immunodeficiency syndrome in an adolescent hemophiliac].	in an adolescent with hemophilia b, the diagnosis of aids was established in face of cachexia, orodigestive candidiasis, associated with lymphopenia, major decrease in t4/t8 ratio with marked decrease in t helper cells and presence of lav-antibodies. the patient died rapidly from a cerebral infection due to toxoplasma gondii. difficulties in diagnosis and treatment of cerebral toxoplasmosis are discussed.	1986	3778094
triiodothyronine (t3) neogenesis in lean and obese la/n-cp rats.	pre-obese la/n-cp rats consumed more food and gained weight more rapidly than their lean littermates, and measures of adipose tissue depots indicated that the excess weight was deposited principally as carcass fat. serum t3 concentrations and resting metabolic rates were lower in corpulent than in lean animals, consistent with a greater efficiency of weight gain in those animals. in vitro measures of t3 neogenesis from t4 were lower in corpulent than in lean animals in liver, kidney, and skeleta ...	1986	3778441
identification of surface-exposed segments of apolipoprotein b-100 in the ldl particle.	the isolation and amino acid sequence of eleven peptides liberated by tryptic treatment from surface-exposed regions of apolipoprotein b-100 in the native low-density lipoprotein particle are described. these peptides represent eight segments in the sequence of the b-100 protein, one of which was localised to the amino-terminal thrombolytic fragment t4 (1297 amino acids), four to the t3 fragment (2052 residues) and three to the carboxylterminal fragment t2 (1287 residues). an exposed segment was ...	1986	3778445
[effect of hormonal and neurotrophic factors on the expression of fast myosin in slow muscle].	myosin atpase and succinic dehydrogenase activity and fast myosin (indirect immunochemical test) were assayed in m. soleus of guinea-pigs after the administration of t4 (200 micrograms/kg) to animals every day for 3 weeks. this was followed by the application of 10 mm colchicine solution to sciatic nerve for 6 min. fast muscle fibers and the line of precipitation with antiserum to fast myosin were revealed in soleus muscle of experimental animals after the application of colchicine and t4 inject ...	1986	3779067
a chemical 5'-phosphorylation of oligodeoxyribonucleotides.	to circumvent the use of t4 polynucleotide kinase and atp for the 5'-phosphorylation of synthetic oligodeoxyribonucleotides after deprotection and purification, we have developed a new chemical phosphorylation reagent that can be used on automated dna synthesis instruments. the phosphite-derived compound, bis(beta-cyanoethoxy)-n,n-diisopropylaminophosphine, is coupled to a solid-supported fragment and oxidized under the same conditions as nucleoside phosphoramidites. the resultant 5'-phosphate o ...	1986	3780373
energy-dependent uptake of 3,5,3'-triiodo-l-thyronine in rat skeletal muscle.	the uptake of [125i]t3 in rat skeletal muscle was investigated by incubating intact soleus muscles with a tracer amount of [125i]t3. at 37 c [125i]t3 uptake increased asymptotically; at 60 min the muscle contained 10% of the total [125i]t3 or 0.238 +/- 0.021% per mg wet tissue. at 0 c the [125i]t3 uptake was 1/5 of that at 37 c. the specific [125i]t3 uptake, determined by subtracting the uptake in the presence of 10 microm unlabeled t3 from the total [125i]t3 uptake, attained a plateau after 60 ...	1986	3780550
effects of semi-starvation and potassium deficiency on the concentration of [3h]ouabain-binding sites and sodium and potassium contents in rat skeletal muscle.	1. using vanadate-facilitated [3h]ouabain binding, the effect of semi-starvation on the total concentration of [3h]ouabain-binding sites was determined in samples of rat skeletal muscle. when 12-week-old rats were semi-starved for 1, 2 or 3 weeks on one-third to half the normal daily energy intake, the [3h]ouabain-binding site concentration in soleus muscle was reduced by 19, 24 and 25% respectively. in extensor digitorum longus, diaphragm and gastrocnemius muscles the decrease after 2 weeks of ...	1986	3676228