Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| influenza and other respiratory virus infections in outpatients with medically attended acute respiratory infection during the 2011-12 influenza season. | respiratory tract infections are a major cause of outpatient visits, yet only a portion is tested to determine the etiologic organism. multiplex reverse transcriptase polymerase chain reaction (mrt-pcr) assays for detection of multiple viruses are being used increasingly in clinical settings. | 2014 | 24852890 |
| human metapneumovirus: review of an important respiratory pathogen. | human metapneumovirus (hmpv), discovered in 2001, most commonly causes upper and lower respiratory tract infections in young children, but is also a concern for elderly subjects and immune-compromised patients. hmpv is the major etiological agent responsible for about 5% to 10% of hospitalizations of children suffering from acute respiratory tract infections. hmpv infection can cause severe bronchiolitis and pneumonia in children, and its symptoms are indistinguishable from those caused by human ... | 2014 | 24841931 |
| viral and host factors determine innate immune responses in airway epithelial cells from children with wheeze and atopy. | airway epithelial cells (aec) from patients with asthma, appear to have an impaired interferon (ifn)-β and -λ response to infection with rhinovirus. | 2014 | 24811725 |
| epidemiology of acute respiratory infections in children in guangzhou: a three-year study. | acute respiratory infections (ari) are some of the most common human diseases worldwide. however, they have a complex and diverse etiology, and the characteristics of the pathogens involved in respiratory infections in developing countries are not well understood. in this work, we analyzed the characteristics of 17 common respiratory pathogens in children (≤14 years old) with ari in guangzhou, southern china over a 3-year period using real-time polymerase chain reaction. pathogens were identifie ... | 2014 | 24797911 |
| comparative epidemiology of human metapneumovirus- and respiratory syncytial virus-associated hospitalizations in guatemala. | human metapneumovirus (hmpv) is an important cause of acute respiratory infections (ari), but little is known about how it compares with respiratory syncytial virus (rsv) in central america. | 2014 | 24761765 |
| alveolar macrophages contribute to the pathogenesis of human metapneumovirus infection while protecting against respiratory syncytial virus infection. | human metapneumovirus (hmpv) and respiratory syncytial virus (rsv) are leading causes of upper and lower respiratory tract infections in young children and among elderly and immunocompromised patients. the pathogenesis of hmpv-induced lung disease is poorly understood. the lung macrophage population consists of alveolar macrophages (ams) residing at the luminal surface of alveoli and interstitial macrophages present within the parenchymal lung interstitium. the involvement of ams in innate immun ... | 2014 | 24749674 |
| the human metapneumovirus small hydrophobic protein has properties consistent with those of a viroporin and can modulate viral fusogenic activity. | human metapneumovirus (hmpv) encodes three glycoproteins: the glycoprotein, which plays a role in glycosaminoglycan binding, the fusion (f) protein, which is necessary and sufficient for both viral binding to the target cell and fusion between the cellular plasma membrane and the viral membrane, and the small hydrophobic (sh) protein, whose function is unclear. the sh protein of the closely related respiratory syncytial virus has been suggested to function as a viroporin, as it forms oligomeric ... | 2014 | 24672047 |
| performance of a rapid multi-analyte 2-photon excitation assay in children with acute respiratory infection. | the purpose of this study is to evaluate the diagnostic performance of the novel 2-photon excitation-based maripoc© assay (arcdia laboratories, turku, finland) for antigen detection of respiratory viruses versus real-time polymerase chain reaction (pcr). the maripoc assay and 2 multiplex real-time pcr techniques were performed on nasopharyngeal samples from pediatric patients with suspicion of acute respiratory infection admitted to a children's hospital in spain during october 2011 to january 2 ... | 2014 | 24661686 |
| the role of influenza and parainfluenza infections in nasopharyngeal pneumococcal acquisition among young children. | animal models suggest that influenza infection favors nasopharyngeal acquisition of pneumococci. we assessed this relationship with influenza and other respiratory viruses in young children. | 2014 | 24621951 |
| respiratory virus infections among children in south china. | acute respiratory tract infection is an important cause of morbidity and mortality with a worldwide disease burden. this study aimed to determine the prevalence and clinical characteristics of children with viral-induced acute respiratory tract infection, in southern china. nasopharyngeal aspirate samples from 1,980 pediatric patients with suspected acute respiratory tract infection, and 82 samples from healthy subject controls were collected for routine examination at the second affiliated hosp ... | 2014 | 24619492 |
| viral aetiology and clinical outcomes in hospitalised infants presenting with respiratory distress. | to determine the prevalence of various types of viruses in infants hospitalised due to respiratory distress, compare molecular diagnostic tests and evaluate symptom severity. | 2014 | 24606114 |
| improving influenza surveillance in portuguese preschool children by parents' report. | influenza surveillance is usually based on nationally organized sentinel networks of physicians and on hospital reports. this study aimed to test a different report system, based on parents' phone contact to the research team and in home collection of samples by a dedicated team. the identification of influenza and other respiratory viruses in children who attended a hospital emergency department was also recorded. real-time pcr and reverse transcription pcr were performed for influenza a and b, ... | 2014 | 24599798 |
| prevalence of respiratory viral infection in children hospitalized for acute lower respiratory tract diseases, and association of rhinovirus and influenza virus with asthma exacerbations. | in this study, we aimed to investigate the prevalence of year-round respiratory viral infection in children with lower respiratory tract infection (lrti) and the relationship between respiratory viral infection and allergen sensitization in exacerbating asthma. | 2014 | 24578714 |
| ten year retrospective evaluation of the seasonal distribution of agent viruses in childhood respiratory tract infections. | infections caused by respiratory viruses sometimes occur as epidemias or pandemias and are an important public health problem in the whole world. these viral agents may lead to severe respiratory diseases especially in young children and in the elderly. the aim of this study was to determine the seasonal distribution of agent viruses in childhood respiratory infections in our region. | 2014 | 26078631 |
| comparison of a new transport medium with universal transport medium at a tropical field site. | limited data are available in rural honduran settings describing the etiology of respiratory infections, partially due to limited specimen transport. a new molecular transport media (mtm) preserves released nucleic acid at ambient temperature for later detection. prospective surveillance was conducted in a honduran clinic to identify 233 children less than 5 years of age presenting with respiratory symptoms. we obtained 2 nasopharyngeal samples and stored 1 in primestore® mtm at room temperature ... | 2014 | 25070008 |
| detection of respiratory viruses in sputum from adults by use of automated multiplex pcr. | respiratory tract infections (rti) frequently cause hospital admissions among adults. diagnostic viral reverse transcriptase pcr (rt-pcr) of nose and throat swabs (nts) is useful for patient care by informing antiviral use and appropriate isolation. however, automated rt-pcr systems are not amenable to utilizing sputum due to its viscosity. we evaluated a simple method of processing sputum samples in a fully automated respiratory viral panel rt-pcr assay (filmarray). archived sputum and nts samp ... | 2014 | 25056335 |
| etiology and clinical outcomes of acute respiratory virus infection in hospitalized adults. | etiologies and clinical profiles of acute respiratory viral infections need to be clarified to improve preventive and therapeutic strategies. | 2014 | 25024868 |
| viral etiologies of hospitalized acute lower respiratory infection patients in china, 2009-2013. | acute lower respiratory infections (alris) are an important cause of acute illnesses and mortality worldwide and in china. however, a large-scale study on the prevalence of viral infections across multiple provinces and seasons has not been previously reported from china. here, we aimed to identify the viral etiologies associated with alris from 22 chinese provinces. | 2014 | 24945280 |
| epidemiology characteristics of respiratory viruses found in children and adults with respiratory tract infections in southern china. | the world health organization (who) ranks respiratory tract infection (rti) as the second leading cause of death worldwide for children under 5 years of age. the aim of this work was to evaluate the epidemiology characteristics of respiratory viruses found in children and adults with rti from july 2009 to june 2012 in southern china. | 2014 | 24927663 |
| single and multipathogen viral infections in hospitalized children with acute respiratory infections. | we aimed to describe the viral etiology of acute respiratory tract infections in children aged 0-8 years admitted to grigore alexandrescu hospital, the largest pediatric hospital in romania. the patients had clinical diagnosis of pneumonia, bronchiolitis or viral respiratory infections and had been hospitalized between september 2010 and september 2011. the study was part of the "molecular investigations of acute respiratory infections caused by non-influenza viruses, to assess the implications ... | 2014 | 24923107 |
| paramyxovirus infection regulates t cell responses by bdca-1+ and bdca-3+ myeloid dendritic cells. | respiratory syncytial virus (rsv) and human metapneumovirus (hmpv), viruses belonging to the family paramyxoviridae, are the most important causes of lower respiratory tract infection in young children. infections with rsv and hmpv are clinically indistinguishable, and both rsv and hmpv infection have been associated with aberrant adaptive immune responses. myeloid dendritic cells (mdcs) play a pivotal role in shaping adaptive immune responses during infection; however, few studies have examined ... | 2014 | 24918929 |
| epidemiology and microbiological investigations of community-acquired pneumonia in children admitted at the emergency department of a university hospital. | the management of children with community-acquired pneumonia (cap) is largely influenced by the development of new molecular diagnostic tests that allow the simultaneous detection of a wide range of pathogens. | 2014 | 24915939 |
| concordance between rt-pcr-based detection of respiratory viruses from nasal swabs collected for viral testing and nasopharyngeal swabs collected for bacterial testing. | epidemiologic studies of respiratory infections frequently rely on separate sample collections for the detection of bacteria and viruses. the requirement for two specimens presents cost, logistical, and acceptability challenges. | 2014 | 24875136 |
| imaging analysis of human metapneumovirus-infected cells provides evidence for the involvement of f-actin and the raft-lipid microdomains in virus morphogenesis. | due to difficulties of culturing human metapneumovirus (hmpv) much of the current understanding of hmpv replication can be inferred from other closely related viruses. the slow rates of virus replication prevent many biochemical analyses of hmpv particles. in this study imaging was used to examine the process of hmpv morphogenesis in individually infected llc-mk2 cells, and to better characterise the sites of hmpv assembly. this strategy has circumvented the problems associated with slow replica ... | 2014 | 25408253 |
| virus profile in children with acute respiratory infections with various severities in beijing, china. | acute respiratory infection (ari) is one of the most common infectious diseases in infants and young children globally. this study aimed to determine the virus profile in children with ari presenting with different severities. | 2014 | 25382323 |
| human respiratory syncytial virus and metapneumovirus in patients with acute respiratory infection in colombia, 2000 - 2011. | to describe the epidemiology of respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) in colombia from 2000 - 2011, including seasonal trends. | 2014 | 25345531 |
| respiratory viral infections and subversion of cellular antioxidant defenses. | reactive oxygen species (ros) formation is part of normal cellular aerobic metabolism, due to respiration and oxidation of nutrients in order to generate energy. low levels of ros are involved in cellular signaling and are well controlled by the cellular antioxidant defense system. elevated levels of ros generation due to pollutants, toxins and radiation exposure, as well as infections, are associated with oxidative stress causing cellular damage. several respiratory viruses, including respirato ... | 2014 | 25584194 |
| human metapneumovirus. | human metapneumovirus (hmpv), a paramyxovirus identified in 2001, is a leading cause of respiratory tract infections in both children and adults. seroprevalence studies demonstrate that the primary infection occurs before the age of 5 years, and humans are reinfected throughout life. the four subgroups of hmpv occur with year-to-year variability, and infection with one subgroup confers some serologic cross-protection. experimental vaccines elicit a humoral response in both animal and human model ... | 2014 | 26104361 |
| other respiratory viruses are important contributors to adult respiratory hospitalizations and mortality even during peak weeks of the influenza season. | during peak weeks of seasonal influenza epidemics, severe respiratory infections without laboratory confirmation are typically attributed to influenza. | 2014 | 25734152 |
| meeting report vlpnpv: session 8: vaccines i. | in session 8 of the recent conference "virus-like particle and nano-particle vaccines" held at the salk institute in la jolla, california (05 june 2014), four scientists described new virus-like particle (vlp) approaches, progress, and early-stage plans for vaccines against significant human pathogens including hpv, malaria, hiv, dengue, and rsv. a unifying theme was that displaying epitopes in an array on a virus-like particle can be a powerful approach for achieving a strong immune response. v ... | 2014 | 25483643 |
| high-quality 3d structures shine light on antibacterial, anti-biofilm and antiviral activities of human cathelicidin ll-37 and its fragments. | host defense antimicrobial peptides are key components of human innate immunity that plays an indispensible role in human health. while there are multiple copies of cathelicidin genes in horses, cattle, pigs, and sheep, only one cathelicidin gene is found in humans. interestingly, this single cathelicidin gene can be processed into different forms of antimicrobial peptides. ll-37, the most commonly studied form, is not only antimicrobial but also possesses other functional roles such as chemotax ... | 2014 | 24463069 |
| viral etiology of respiratory infections in children in southwestern saudi arabia using multiplex reverse-transcriptase polymerase chain reaction. | to investigate 15 respiratory viruses in children with acute respiratory tract infections (artis) using multiplex reverse-transcriptase polymerase chain reaction (rt-pcr), and to analyze the clinical and epidemiological features of these viruses. | 2014 | 25399211 |
| characterization of human coronavirus oc43 and human coronavirus nl63 infections among hospitalized children <5 years of age. | multiplex molecular assays now make it possible for clinical laboratories to detect human coronaviruses (hcovs). we investigated the clinical characteristics of hcov-oc43 and hcov-nl63 in patients <5 years of age during a recent coronavirus season. | 2014 | 24577040 |
| il-27r-mediated regulation of il-17 controls the development of respiratory syncytial virus-associated pathogenesis. | il-27 is a heterodimeric cytokine composed of the subunits p28 and epstein-barr virus induced gene (ebi)-3 and is known for its effects on t-cell function and differentiation. il-27 signals through the widely expressed il-27 receptor (il-27r), composed of the ligand-specific il-27rα chain and gp130. engagement of the il-27r activates stat1 signaling, induces the expression of the type 1 helper t-cell (th1) cytokine, interferon γ, and suppresses the differentiation of th2 and th17 cells. this stu ... | 2014 | 24726498 |
| respiratory viruses within homeless shelters in marseille, france. | homeless shelters are identified as places where humans are at high risk of acquiring respiratory disease. we previously reported the prevalence of the main respiratory diseases affecting a population of homeless in marseille, france. here, we investigated the prevalence of 10 respiratory viruses in a similar homeless population during 2 successive winter seasons. | 2014 | 24499605 |
| an outbreak of acute respiratory disease in china caused by human adenovirus type b55 in a physical training facility. | to investigate the cause of an acute respiratory tract infection (arti) outbreak. | 2014 | 25236387 |
| risk factors of prolonged hospital stay in children with viral severe acute respiratory infections. | severe acute lower respiratory infections (saris) are one of the major causes of morbidity and mortality in young children, especially in developing countries. the present study focused on detection of risk factors for prolonged hospital stays among children with viral saris. | 2014 | 25313605 |
| genetic vaccine for respiratory syncytial virus provides protection without disease potentiation. | respiratory syncytial virus (rsv) is a major cause of infectious lower respiratory disease in infants and the elderly. as there is no vaccine for rsv, we developed a genetic vaccine approach that induced protection of the entire respiratory tract from a single parenteral administration. the approach was based on adenovirus vectors derived from newly isolated nonhuman primate viruses with low seroprevalence. we show for the first time that a single intramuscular (im) injection of the replication- ... | 2014 | 23752342 |
| synthesis and antiviral activity of new phenylimidazopyridines and n-benzylidenequinolinamines derived by molecular simplification of phenylimidazo[4,5-g]quinolines. | continuing our program of research concerning the antiviral activity of a wide series of new angular and linear azolo bicyclic and tricyclic derivatives, now we have simplified and modified the 4-chloro-2-(4-nitrophenyl)-3h-imidazo[4,5-g]quinoline 1, which previously resulted the most active derivative, through either the elimination of the central ring or the opening of the imidazole ring, obtaining various imidazopyridines and n-benzylidenequinolinamines respectively. title compounds were test ... | 2014 | 25014745 |
| phenolic compounds from origanum vulgare and their antioxidant and antiviral activities. | in the present study, six new phenolic compounds (1-6) along with five known ones were isolated from the ethanol extract of the whole plants of origanum vulgare. the structures of the new compounds were identified on the basis of extensive spectroscopic analyses (uv, ir, nmr, and hresims) and acid hydrolysis. twenty-one phenolic compounds isolated from o. vulgare in our previous and present studies were evaluated for their in vitro antioxidant activity using 2,2-diphenyl-1-picryhydrazyl (dpph) r ... | 2014 | 24444941 |
| agmatine-containing poly(amidoamine)s as a novel class of antiviral macromolecules: structural properties and in vitro evaluation of infectivity inhibition. | poly(amidoamine)s (paas) are multifunctional tert-amine polymers endowed with high structural versatility. here we report on the screening of a minilibrary of paas against a panel of viruses. the paa agma1 showed antiviral activity against herpes simplex virus, human cytomegalovirus, human papillomavirus 16, and respiratory syncytial virus but not against human rotavirus and vesicular stomatitis virus. the results suggest the contribution of both a polycationic nature and side guanidine groups i ... | 2014 | 25092704 |
| [nosocomial respiratory viral infection]. | nosocomial viral infections have not been well studied. in this context, the aim of this work is to review epidemiological and therapeutic data published on the main viral agents that can produce nosocomial respiratory infection. the study thus aims to expand knowledge of behaviour of these agents in nosocomial infections and provide information to improve the implementation of preventive measures. the concepts of mimivirus, herpes simplex virus, varicella-zoster virus, cytomegalovirus, respirat ... | 2014 | 25189985 |
| human respiratory coronaviruses detected in patients with influenza-like illness in arkansas, usa. | acute respiratory viruses often result in significant morbidity and mortality. the potential impact of human respiratory coronavirus (cov) infections was underestimated until the severe acute respiratory syndrome (sars-cov) outbreak in 2003, which showed that new, highly pathogenic coronaviruses could be introduced to humans, highlighting the importance of monitoring the circulating coronaviruses. the use of sensitive molecular methods has contributed to the differential diagnosis of viruses cir ... | 2014 | 27588218 |
| [detection of respiratory viruses in influenza-like illness in shijiazhuang, china in 2011]. | this study aimed to investigate viral infections and the prevalence of influenza-like illness (ili) in shijiazhuang, china, in 2011 and to provide a scientific basis for the diagnosis and control of respiratory tract infections. throat swab specimens were collected from 483 cases of ili who were outpatients in the influenza surveillance sentinel hospitals in shijiazhuang between january and december 2011. all specimens were examined by multiplex rt-pcr for the following 15 respiratory tract viru ... | 2014 | 25272592 |
| successful systemic high-dose ribavirin treatment of respiratory syncytial virus-induced infections occurring pre-engraftment in allogeneic hematopoietic stem cell transplant recipients. | respiratory syncytial virus (rsv) is a frequent cause of respiratory tract infectious disease (rtid) in allogeneic hematopoietic stem cell transplant (hsct) recipients associated with a high mortality once infection has progressed from upper rtid (urtid) to lower rtid (urtid). aerosolized ribavirin (rbv) is considered a cornerstone of treatment, but is expensive and has toxic side effects on patients and staff. in this study, rsv infection was detected by polymerase chain reaction (pcr) from rou ... | 2013 | 23692664 |
| development of a new resequencing pathogen microarray based assay for detection of broad-spectrum respiratory tract viruses in patients with community-acquired pneumonia. | a resequencing pathogen microarray (rpm) is a single, highly multiplexed assay for detecting and differentiating similarly related pathogens by using closely overlapping probe sets to determine a target organism's nucleotide sequence. in this study, a new rpm (rpm-ivdc1) that consisted of 224-bp detector tiles corresponding to 9 influenza a subtypes, 11 rhinoviruses, 28 enteroviruses and 38 other respiratory viruses was developed and optimized to provide individual and simultaneous detection sen ... | 2013 | 24086618 |
| [late onset streptococcus agalactiae disease associated with respiratory syncytial virus infections in very premature newborn]. | 2013 | 23582195 | |
| comparison of initial feasibility of host cell lines for viral vaccine production. | in order to reduce the time required for the development and production of viral vaccines, host cell lines should be available as expression systems for production of viral vaccines against groups of viral pathogens. a selection of cell lines was compared for their initial feasibility as expression system for the replication of polioviruses, influenza a viruses and respiratory syncytial virus (wild type strain a2). six adherent cell lines (vero, hek-293, mrc-5, cho-k1, bhk-21 c13, mdck) and six ... | 2013 | 23684847 |
| highly immunostimulatory rna derived from a sendai virus defective viral genome. | defective viral genomes (dvgs) are generated during virus replication. dvgs bearing complementary ends are strong inducers of dendritic cell (dc) maturation and of the expression of antiviral and pro-inflammatory cytokines by triggering signaling of the rig-i family of intracellular pattern recognition receptors. our data show that dcs stimulated with virus containing dvgs have an enhanced ability to activate human t cells and can induce adaptive immunity in mice. in addition, we describe the ge ... | 2013 | 24099876 |
| pneumococcal bacterial load colonization as a marker of mixed infection in children with alveolar community-acquired pneumonia and respiratory syncytial virus or rhinovirus infection. | the main aim of this study was to evaluate whether nasopharyngeal streptococcus pneumoniae colonization in children with alveolar community-acquired pneumonia (cap) and respiratory syncytial virus (rsv) or rhinovirus (rv) infection indicates a mixed lung infection. | 2013 | 23743541 |
| rt-pcr detection of respiratory pathogens in newborn children admitted to a neonatal medium care unit. | this study aimed to determine the prevalence of respiratory pathogens among newborns admitted to a neonatal medium care unit (nmcu) and to identify clinical predictors. | 2013 | 23202720 |
| viral and bacterial causes of severe acute respiratory illness among children aged less than 5 years in a high malaria prevalence area of western kenya, 2007-2010. | few comprehensive data exist on the etiology of severe acute respiratory illness (sari) among african children. | 2013 | 22914561 |
| non-propagating, recombinant vesicular stomatitis virus vectors encoding respiratory syncytial virus proteins generate potent humoral and cellular immunity against rsv and are protective in mice. | respiratory syncytial virus (rsv) is a major cause of severe lower respiratory tract illness in infants, the elderly, and other high-risk individuals. despite years of research in this field, there is no effective licensed vaccine to prevent rsv infection. we have generated candidate rsv vaccines using a recombinant vesicular stomatitis virus (rvsv) replicon in which the attachment and fusion domains of the vsv glycoprotein (g) have been deleted (rvsv-gstem), rendering the virus propagation-defe ... | 2013 | 23261719 |
| development of a simple, rapid, sensitive, high-throughput luciferase reporter based microneutralization test for measurement of virus neutralizing antibodies following respiratory syncytial virus vaccination and infection. | we have established a new reporter gene-based rsv neutralization test using renilla luciferase. the rsv-luciferase neutralization test (rsv-luc-neut) is a simple, rapid, high throughput, and less labor intensive functional serological assay than the traditional rsv-prnt, capable of measuring a broad range of anti-rsv neutralizing antibodies targeting both rsv-f and rsv-g proteins. specificity and sensitivity of the rsv-luc-neut are comparable to the rsv-prnt. panels of pre-vaccination and post-v ... | 2013 | 23742994 |
| in vivo evaluation of antiviral compounds on respiratory syncytial virus using a juvenile vervet monkey (chlorocebus pygerythrus) infection model. | several animal models with varying susceptibilities to respiratory syncytial virus (rsv) infection have been developed to study the specific aspects of rsv disease. many of these models are used for testing antiviral compounds or in vaccine efficacy studies during preclinical evaluation. in this chapter, we describe the study design of an efficacy study of an rsv inhibitor, performed in a juvenile vervet monkey model for rsv. | 2013 | 23821283 |
| lack of effect of bovine lactoferrin in respiratory syncytial virus replication and clinical disease severity in the mouse model. | lactoferrin (lf) is a glycoprotein present in human milk with known antimicrobial effects. in vitro, lf has demonstrated antiviral activity against respiratory syncytial virus (rsv). we sought to assess the effect of bovine (b)lf in rsv replication, lung inflammation and function, cytokine profiles and clinical disease in an in vivo murine model. | 2013 | 23735300 |
| respiratory syncytial virus--a comprehensive review. | respiratory syncytial virus (rsv) is amongst the most important pathogenic infections of childhood and is associated with significant morbidity and mortality. although there have been extensive studies of epidemiology, clinical manifestations, diagnostic techniques, animal models and the immunobiology of infection, there is not yet a convincing and safe vaccine available. the major histopathologic characteristics of rsv infection are acute bronchiolitis, mucosal and submucosal edema, and luminal ... | 2013 | 23575961 |
| epidemiological and genetic characteristics associated with the severity of acute viral bronchiolitis by respiratory syncytial virus. | to assess the epidemiological and genetic factors associated with severity of acute viral bronchiolitis (avb) by respiratory syncytial virus (rsv). | 2013 | 24035870 |
| genetic replacement of surfactant protein-c reduces respiratory syncytial virus induced lung injury. | individuals with deficiencies of pulmonary surfactant protein c (sp-c) develop interstitial lung disease (ild) that is exacerbated by viral infections including respiratory syncytial virus (rsv). sp-c gene targeted mice (sftpc -/-) lack sp-c, develop an ild-like disease and are susceptible to infection with rsv. | 2013 | 23399055 |
| [application of filmarray assay for detection of respiratory tract infections in immunocompromised persons]. | a variety of viruses and bacteria are responsible for acute upper and lower respiratory tract infections worldwide. severe and even fatal disease can occur especially in group ofimmunocompromised individuals. accurate pathogen identification allows clinicians to determine the need for ancillary diagnostic testing, antibacterial and/or antiviral therapy and can motivate decisions regarding hospitalization and infection control measures. | 2013 | 24432557 |
| challenges and opportunities for respiratory syncytial virus vaccines. | respiratory syncytial virus (rsv) causes a significant proportion of the global burden of respiratory disease. here we summarize the conclusions of a series of chapters written by investigators describing and interpreting what is known about the virology, clinical manifestations, immunity, pathogenesis, and epidemiology of rsv relevant to vaccine development. several technological and conceptual advances have recently occurred that make rsv vaccine development more feasible, and this collected k ... | 2013 | 24362701 |
| human airway epithelial cell cultures for modeling respiratory syncytial virus infection. | respiratory syncytial virus (rsv) is an important human respiratory pathogen with narrow species tropism. limited availability of human pathologic specimens during early rsv-induced lung disease and ethical restrictions for rsv challenge studies in the lower airways of human volunteers has slowed our understanding of how rsv causes airway disease and greatly limited the development of therapeutic strategies for reducing rsv disease burden. our current knowledge of rsv infection and pathology is ... | 2013 | 24362700 |
| the mouse model of respiratory syncytial virus disease. | the laboratory mouse is the species of choice for most immunological studies, ranging from simple vaccine testing to the intricate dissection of fundamental immunopathogenic mechanisms. although not fully mouse adapted, some strains of respiratory syncytial virus (rsv) replicate in the murine respiratory tract and induce specific t and b cell responses. passive transfer of neutralising antibody is protective and assist in viral clearance. in addition, many of rsv's complex behaviours are recapit ... | 2013 | 24362699 |
| the cotton rat sigmodon hispidus model of respiratory syncytial virus infection. | the cotton rat sigmodon hispidus is a new world rodent that has become an important model of respiratory syncytial virus (rsv) infection. this small animal is relatively permissive to rsv and can be infected throughout life. it recapitulates the pathology associated with the fi-rsv vaccine-enhanced disease, the phenomenon of maternally transmitted immunity and the ability of passive immunity to suppress efficacy of rsv vaccines. different highly susceptible human cohort scenarios have been model ... | 2013 | 24362698 |
| subunit and virus-like particle vaccine approaches for respiratory syncytial virus. | despite its impact on global health, there is no vaccine available for the prevention of respiratory syncytial virus (rsv) infection. failure to develop a licensed vaccine is not due to lack of effort, as numerous vaccine candidates have been characterized in preclinical and clinical studies spanning five decades. the vaccine candidates thus far explored can be generally divided into four categories: (1) whole inactivated virus, (2) replication competent, attenuated virus including recombinant v ... | 2013 | 24362695 |
| live-attenuated respiratory syncytial virus vaccines. | live-attenuated respiratory syncytial virus (rsv) vaccines offer several advantages for immunization of infants and young children: (1) they do not cause vaccine-associated enhanced rsv disease; (2) they broadly stimulate innate, humoral, and cellular immunity, both systemically and locally in the respiratory tract; (3) they are delivered intranasally; and (4) they replicate in the upper respiratory tract of young infants despite the presence of passively acquired maternally derived rsv neutrali ... | 2013 | 24362694 |
| respiratory syncytial virus disease: prevention and treatment. | respiratory syncytial virus (rsv) is one of the most clinically important viruses infecting young children, the elderly, and the immunocompromised. over the past decade, the most significant advance in the prevention of rsv disease has been the development of high-titered antibody products. infection control is the only other strategy to prevent rsv disease. a humanized monoclonal antibody directed against the fusion (f) protein palivizumab, (synagis®, medimmune, inc., gaithersburg, md), is give ... | 2013 | 24362693 |
| consequences of immature and senescent immune responses for infection with respiratory syncytial virus. | infants in the first 6 months of life and older adults (>65 years of age) are disproportionately burdened with respiratory syncytial virus (rsv)-associated morbidity and mortality. while other factors play a role in the risk these groups assume, shortcomings of the immune response make a substantial contribution to the predisposition to severe disease. ineffectual antibody production with misdirected cytokine responses and excess inflammation in the airways are common to both groups. however, th ... | 2013 | 24362692 |
| host gene expression and respiratory syncytial virus infection. | advances in rna interference (rnai) and transcription studies have facilitated the application of systematic cell-based loss- or gain-of-function and cell response screening that enable genome-wide analysis of cell factors involved in viral replication and disease. application of both experimental and computational biology approaches have led to crucial insights into virus infection, its life cycle, and host gene targets for disease intervention. a better understanding of the spatial and tempora ... | 2013 | 24362691 |
| respiratory syncytial virus mechanisms to interfere with type 1 interferons. | respiratory syncytial virus (rsv) is a member of the paramyxoviridae family that consists of viruses with nonsegmented negative-strand rna genome. infection by these viruses triggers the innate antiviral response of the host, mainly type i interferon (ifn). essentially all other viruses of this family produce ifn suppressor functions by co-transcriptional rna editing. in contrast, rsv has evolved two unique nonstructural proteins, ns1 and ns2, to effectively serve this purpose. together, ns1 and ... | 2013 | 24362690 |
| the adaptive immune response to respiratory syncytial virus. | respiratory syncytial virus (rsv) causes severe respiratory disease in children, the elderly and immunocompromised individuals. the combined actions of cd4 and cd8 t cells play a critical role in terminating an acute rsv infection whereas antibodies can provide protection from re-infection. despite eliciting an immune response that mediates clearance of the virus, immunity to the virus appears to wane over time and individuals remain susceptible to reinfection with rsv throughout their lifetime. ... | 2013 | 24362689 |
| innate immune responses to respiratory syncytial virus infection. | the innate immune response has a critical role in the initial stages of respiratory syncytial virus (rsv) infection and provides important instructional control that determines the direction of the acquired immune response and the severity of subsequent disease. contributions to innate immunity include responses initiated in epithelial cells, dendritic cells, and macrophages. the initiation and the intensity of the response depends upon the recognition of pathogen-associated molecular patterns ( ... | 2013 | 24362688 |
| human genetics and respiratory syncytial virus disease: current findings and future approaches. | infection with respiratory syncytial virus (rsv) can result in a wide spectrum of pulmonary manifestations, from mild upper respiratory symptoms to severe bronchiolitis and pneumonia. although there are several known risk factors for severe rsv disease, namely, premature birth, chronic lung disease, congenital heart disease, and t cell immunodeficiency, the majority of young children who develop severe rsv disease are otherwise healthy children. genetic susceptibility to rsv infection is emergin ... | 2013 | 24362687 |
| respiratory syncytial virus and reactive airway disease. | reactive airway disease (rad) is a general term for respiratory illnesses manifested by wheezing. respiratory syncytial virus (rsv) results in wheezing, either by causing bronchiolitis or by inducing acute exacerbations of asthma. there has been a long-standing interest in whether severe rsv bronchiolitis in infancy is a risk factor for the development of asthma later in childhood. while epidemiologic studies have suggested that such a link exists, a very recent study suggests that infants with ... | 2013 | 24362686 |
| structure and function of respiratory syncytial virus surface glycoproteins. | the two major glycoproteins on the surface of the respiratory syncytial virus (rsv) virion, the attachment glycoprotein (g) and the fusion glycoprotein (f), control the initial phases of infection. g targets the ciliated cells of the airways, and f causes the virion membrane to fuse with the target cell membrane. the f protein is the major target for antiviral drug development, and both g and f glycoproteins are the antigens targeted by neutralizing antibodies induced by infection. in this chapt ... | 2013 | 24362685 |
| influence of respiratory syncytial virus strain differences on pathogenesis and immunity. | molecular epidemiology studies have provided convincing evidence of antigenic and sequence variability among respiratory syncytial virus (rsv) isolates. circulating viruses have been classified into two antigenic groups (a and b) that correlate with well-delineated genetic groups. most sequence and antigenic differences (both inter- and intra-groups) accumulate in two hypervariable segments of the g-protein gene. sequences of the g gene have been used for phylogenetic analyses. these studies hav ... | 2013 | 24362684 |
| clinical and epidemiologic features of respiratory syncytial virus. | since its discovery in 1955, respiratory syncytial virus (rsv) has consistently been noted to be the single most important cause of lower respiratory tract illness in infants <1 year of age. rsv also causes repeat infections and significant disease throughout life. in addition to the young child, persons with compromised immune, pulmonary or cardiac systems, and the elderly have significant risk from infection. though rsv causes the full spectrum of acute respiratory illnesses, it is most notabl ... | 2013 | 24362683 |
| apnea with rsv infection in three infants receiving palivizumab. | the present report describes three infants receiving palivizumab prophylaxis who presented with apnea associated with respiratory syncytial virus (rsv) infection. all three were found to be rsv positive but had mild bronchiolitis courses. even though palivizumab has been shown to be an effective prophylaxis in preventing rsv bronchiolitis hospitalizations, its effect on apnea is unknown. the cases presented raise the concern that apnea associated with rsv must still be considered in infants who ... | 2013 | 24330303 |
| vlcad deficiency in a patient who recovered from ventricular fibrillation, but died suddenly of a respiratory syncytial virus infection. | vlcad deficiency is an autosomal recessive disorder caused by a defect of fatty acid oxidation. the phenotype is classified into three clinical forms on the basis of the onset of symptoms: a severe form with neonatal onset; a milder form with childhood onset; and a late-onset form. the neonatal form is the most common, and has a higher mortality rate than the others. we report the case of a newborn infant with vlcad deficiency who developed ventricular fibrillation, which was successfully treate ... | 2013 | 24330285 |
| outbreak of respiratory syncytial virus infections in a nursing home and possible sources of introduction: the netherlands, winter 2012/2013. | 2013 | 24329823 | |
| emergency department surveillance as a proxy for the prediction of circulating respiratory viral disease in eastern ontario. | seasonal outbreaks of winter respiratory viruses are responsible for increases in morbidity and mortality in the community. previous studies have used hospitalizations, intensive care unit and emergency department (ed) visits as indicators of seasonal influenza incidence. | 2013 | 24421826 |
| a model of the costs of community and nosocomial pediatric respiratory syncytial virus infections in canadian hospitals. | approximately one in 10 hospitalized patients will acquire a nosocomial infection (ni) after admission to hospital, of which 71% are due to respiratory viruses, including the respiratory syncytial virus (rsv). nis are concerning and lead to prolonged hospitalizations. the economics of nis are typically described in generalized terms and specific cost data are lacking. | 2013 | 24421788 |
| perinatal exposure to insecticide methamidophos suppressed production of proinflammatory cytokines responding to virus infection in lung tissues in mice. | methamidophos, a representative organophosphate insecticide, is regulated because of its severe neurotoxicity, but it is suspected of contaminating agricultural foods in many countries due to illicit use. to reveal unknown effects of methamidophos on human health, we evaluated the developmental immunotoxicity of methamidophos using a respiratory syncytial virus (rsv) infection mouse model. pregnant mice were exposed to methamidophos (10 or 20 ppm) in their drinking water from gestation day 10 to ... | 2013 | 24369005 |
| decrease in formalin-inactivated respiratory syncytial virus (fi-rsv) enhanced disease with rsv g glycoprotein peptide immunization in balb/c mice. | respiratory syncytial virus (rsv) is a high priority target for vaccine development. one concern in rsv vaccine development is that a non-live virus vaccine would predispose for enhanced disease similar to that seen with the formalin inactivated rsv (fi-rsv) vaccine. since a mab specific to rsv g protein can reduce pulmonary inflammation and eosinophilia seen after rsv infection of fi-rsv vaccinated mice, we hypothesized that rsv g peptides that induce antibodies with similar reactivity may limi ... | 2013 | 24376637 |
| the impact of viral genotype on pathogenesis and disease severity: respiratory syncytial virus and human rhinoviruses. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract infection (lri) and viral death in infants. rsv disease in infants is characterized by epithelial desquamation, neutrophilic bronchiolitis and pneumonia and obstructive pulmonary mucus. human rhinoviruses (hrvs) are by far the most common cause of symptomatic upper respiratory tract infection (uri) in people and are more recently appreciated as a significant cause of lri. rsv and hrv are also implicated in asthma p ... | 2013 | 24455766 |
| [relevant pathogenesis of heat and phlegm in infantile viral pneumonia: an analysis by association rules]. | to study the application of association rules in chinese medical pathogeneses and pathologies of heat and phlegm in infantile viral pneumonia. | 2013 | 24483108 |
| risk factors for hospitalization due to lower respiratory tract infection in preterm infants on palivizumab prophylaxis. | to determine the risk factors associated with lower respiratory tract infections (lrti) related hospitalizations in preterm infants receiving palivizumab throughout the high season for respiratory syncytial virus (rsv) infection. | 2013 | 24910750 |
| hospitalization for respiratory syncytial virus bronchiolitis and disease severity in twins. | respiratory syncytial virus (rsv) is a common cause of lower respiratory tract disease and hospitalization in infants and young children. infants of multiple births, who are often premature, might be more susceptible to developing a more severe rsv infection than singletons. | 2013 | 24511652 |
| exhaled nitric oxide is related to atopy, but not asthma in adolescents with bronchiolitis in infancy. | the fraction of exhaled nitric oxide (feno) has been suggested as a non-invasive marker of eosinophilic inflammation in asthma, but lately rather as a biomarker of atopy than of asthma itself. asthma after bronchiolitis is common up to early adolescence, but the inflammation and pathophysiology may differ from other phenotypes of childhood asthma. we aimed to assess if feno was different in children with former hospitalization for bronchiolitis and a control group, and to explore whether the rol ... | 2013 | 24237793 |
| effect of brazilian propolis on exacerbation of respiratory syncytial virus infection in mice exposed to tetrabromobisphenol a, a brominated flame retardant. | tetrabromobisphenol a (tbbpa), a brominated flame retardant, has been found to exacerbate pneumonia in respiratory syncytial virus- (rsv-) infected mice. we examined the effect of brazilian propolis (af-08) on the exacerbation of rsv infection by tbbpa exposure in mice. mice were fed a powdered diet mixed with 1% tbbpa alone, 0.02% af-08 alone, or 1% tbbpa and 0.02% af-08 for four weeks and then intranasally infected with rsv. tbbpa exposure increased the pulmonary virus titer and level of ifn- ... | 2013 | 24250719 |
| viral agents causing acute lower respiratory tract infections in hospitalized children at a tertiary care center in saudi arabia. | to determine the load and importance of respiratory viruses in hospitalized saudi children with acute lower respiratory tract infections (alris). | 2013 | 24252893 |
| unmet needs in respiratory diseases : "you can't know where you are going until you know where you have been"--anonymous. | the care of patients with respiratory diseases has improved vastly in the past 50 years. in spite of that, there are still massive challenges that have not been resolved. although the incidence of tuberculosis has decreased in the developed world, it is still a significant public health problem in the rest of the world. there are still over 2 million deaths annually from tuberculosis, with most of these occurring in the developing world. even with the development of new pharmaceuticals to treat ... | 2013 | 24293395 |
| community household income and resource utilization for common inpatient pediatric conditions. | child health is influenced by biomedical and socioeconomic factors. few studies have explored the relationship between community-level income and inpatient resource utilization for children. our objective was to analyze inpatient costs for children hospitalized with common conditions in relation to zip code-based median annual household income (hhi). | 2013 | 24276839 |
| a gene expression signature for rsv: clinical implications and limitations. | peter openshaw discusses the challenges in advancing respiratory syncytial virus (rsv) treatments and the implications of a study by mejias and colleagues using a newly identified gene signature for diagnosis and prediction of rsv severity. please see later in the article for the editors' summary. | 2013 | 24265600 |
| whole blood gene expression profiles to assess pathogenesis and disease severity in infants with respiratory syncytial virus infection. | respiratory syncytial virus (rsv) is the leading cause of viral lower respiratory tract infection (lrti) and hospitalization in infants. mostly because of the incomplete understanding of the disease pathogenesis, there is no licensed vaccine, and treatment remains symptomatic. we analyzed whole blood transcriptional profiles to characterize the global host immune response to acute rsv lrti in infants, to characterize its specificity compared with influenza and human rhinovirus (hrv) lrti, and to ... | 2013 | 24265599 |
| respiratory syncytial virus circulation in seven countries with global disease detection regional centers. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract infections in young children globally, with the highest burden in low- and middle-income countries where the association between rsv activity and climate remains unclear. | 2013 | 24265484 |
| hospitalizations for acute lower respiratory tract infection due to respiratory syncytial virus in thailand, 2008-2011. | few population-based estimates of the incidence of respiratory syncytial virus (rsv) infection in low- or middle-income countries are available. we describe the incidence and epidemiology of hospitalizations for rsv-associated acute lower respiratory tract infection (alri) detected by active population-based surveillance in 2 rural thailand provinces during 2008-2011. | 2013 | 24265483 |
| replacement and positive evolution of subtype a and b respiratory syncytial virus g-protein genotypes from 1997-2012 in south africa. | of the respiratory syncytial virus (rsv) genotypes previously described in south africa during 1997-2002, only ga2 and ga5 persisted until 2006, with ba having replaced all previous rsv-b genotypes. this poses the question whether rsv-a is more stable than rsv-b and whether positive selection drives evolution of genotypes. | 2013 | 24265482 |
| epidemiology of respiratory syncytial virus infection in rural and urban kenya. | information on the epidemiology of respiratory syncytial virus (rsv) infection in africa is limited for crowded urban areas and for rural areas where the prevalence of malaria is high. | 2013 | 24265480 |
| respiratory syncytial virus infection in guatemala, 2007-2012. | respiratory syncytial virus (rsv) is a major cause of acute respiratory illness (ari). little is known about rsv disease among older children and adults in central america. | 2013 | 24265479 |