Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
identification, genetic diversity and cereulide producing ability of bacillus cereus group strains isolated from beninese traditional fermented food condiments.bacillus cereus sensu lato is often detected in spontaneously fermented african foods but is rarely identified to species level. only some of the b. cereus group species are reported to be pathogenic to humans and identification to species level is necessary to estimate the safety of these products. in the present study, a total of 19 bacillus cereus group spp. isolated from afitin, iru and sonru, three spontaneously fermented african locust (parkia biglobosa) bean based condiments produced in b ...201020599285
conjugal transfer between bacillus thuringiensis and bacillus cereus strains is not directly correlated with growth of recipient strains.bacillus thuringiensis and bacillus cereus belong to the b. cereus species group. the two species share substantial chromosomal similarity and differ mostly in their plasmid content. the phylogenetic relationship between these species remains a matter of debate. there is genetic exchange both within and between these species, and current evidence indicates that insects are a particularly suitable environment for the growth of and genetic exchange between these species. we investigated the conjug ...201020600090
aerobic biogenesis of selenium nanospheres by bacillus cereus isolated from coalmine soil.microorganisms that are exposed to pollutants in the environment, such as metals/metalloids, have a remarkable ability to fight the metal stress by various mechanisms. these metal-microbe interactions have already found an important role in biotechnological applications. it is only recently that microorganisms have been explored as potential biofactories for synthesis of metal/metalloid nanoparticles. biosynthesis of selenium (se 0) nanospheres in aerobic conditions by a bacterial strain isolate ...201020602763
[clinical relevance of time-to-positivity in bactec9240 blood culture system].continuous monitoring systems have allowed determination of the time-to-positivity (ttp). we evaluated the clinical relevance of ttp in the bactec9240 system (becton-dickinson, usa).201020603588
crystallization and preliminary x-ray analysis of a phosphopentomutase from bacillus cereus.phosphopentomutases (ppms) interconvert d-ribose 5-phosphate and alpha-d-ribose 1-phosphate to link glucose and nucleotide metabolism. ppm from bacillus cereus was overexpressed in escherichia coli, purified to homogeneity and crystallized. bacterial ppms are predicted to contain a di-metal reaction center, but the catalytically relevant metal has not previously been identified. sparse-matrix crystallization screening was performed in the presence or absence of 50 mm mncl(2). this strategy resul ...201020606280
anaerobic benzene biodegradation by a pure bacterial culture of bacillus cereus under nitrate reducing conditions.a pure culture using benzene as sole carbon and energy sources was isolated by screening procedure from gasoline contaminated soil. the analysis of the 16s rdna gene sequence, morpholpgical and physiological characteristics showed that the isolated strain was a member of genus bacillus cereus. the biodegradation performance of benzene by b. cereus was evaluated, and the results showed that benzene could be efficiently biodegraded when the initial benzene concentration was below 150 mg/l. the met ...201020608507
chemical composition and biological activities of santiria trimera (burseraceae) essential oils from gabon.water-distilled essential oils from leaves and bark of santiria trimera (oliv.) aubrév. (burseraceae) collected in gabon were analyzed using capillary gc-fid and gc-ms. the leaf essential oil, which is described for the first time, was dominated by sesquiterpenes (76.5%), among which alpha-humulene (34.6%) and beta-caryophyllene (14.9%) were the major components. the bark essential oil was almost exclusively monoterpenic, with alpha-pinene (51.5%) and alpha-terpineol (16.8%) as main constituents ...201020614835
comparison of enterotoxin production and phenotypic characteristics between emetic and enterotoxic bacillus cereus.bacillus cereus was divided into emetic toxin (cereulide)- and enterotoxin-producing strains, but emetic toxin-producing b. cereus is difficult to detect immunochemically. screening methods for emetic toxin-producing b. cereus are needed. the objectives of this study were to identify and detect emetic toxin-producing b. cereus among 160 b. cereus strains, and to compare enterotoxin production and phenotypic characteristics between the emetic toxin-producing and enterotoxin-producing strains. for ...201020615333
aureocins 4185, bacteriocins produced by staphylococcus aureus 4185: potential application in food preservation.in the present study, the bacteriocins produced by staphylococcus aureus 4185, a strain isolated from bovine mastitis, were purified and partially characterized. after purification by ammonium sulfate precipitation, cation-exchange chromatography, and five runs of high-performance liquid chromatography (hplc), antimicrobial activity was recovered with 40% and 80% isopropanol, suggesting that more than one antimicrobial peptide, named aureocins 4185, is produced by s. aureus 4185. mass spectromet ...201020618078
prevalence and diversity of insertion sequences in the genome of bacillus thuringiensis ybt-1520 and comparison with other bacillus cereus group members.members of the bacillus cereus group are closely related bacteria that exhibit highly divergent pathogenic properties. sequencing of bacillus thuringiensis ssp. kurstaki strain ybt-1520 revealed an increased number of insertion sequences (iss) compared with those of the published b. cereus group genomes. although some of these iss have been observed and summarized in b. thuringiensis previously, a genomic characterization of their content is required to reveal their distribution and evolution. t ...201020618853
purification and characterization of chitinase from a new species strain pseudomonas sp. tku008.the chitinase producing strain tku008 was isolated from the soil in taiwan, and it was identified as a new species of pseudomonas. the culture condition suitable for production of chitinase was found to be shaken at 30 degrees c for 4 days in 100 ml of medium containing 1% shrimp and crab shell powder, 0.1 % k2hpo4 and 0.05% mgso4 . 7 h2o (ph 7). the tku008 chitinase was suppressed by the simultaneously existing protease, which also showed the maximum activity at the forth day of incubation. the ...201020622499
glucosamine-functionalized silver glyconanoparticles: characterization and antibacterial activity.we report the analytical and in vitro antibacterial activity of glucosamine-functionalized silver glyconanoparticles. morphological characterization ensured the surface topography and particle size distribution of both silver and glucosamine-silver nanoparticles. surface plasmon resonance of both types of nanoparticle was determined from uv-visible spectroscopy using four different sample concentrations (10-40 μl). the resulting functionalized glyconanoparticles show maximum absorbance with a re ...201020623220
cytotoxicity of the bacillus cereus nhe enterotoxin requires specific binding order of its three exoprotein components.this study focuses on the interaction of the three components of the bacillus cereus nhe enterotoxin with particular emphasis on the functional roles of nheb and nhec. the results demonstrated that both nheb and nhec were able to bind to vero cells directly while nhea lacked this ability. it was also shown that nhe-induced cytotoxicity required a specific binding order of the individual components whereby the presence of nhec in the priming step as well as the presence of nhea in the final incub ...201020624910
synthesis and evaluation of novel phosphate ester analogs as neutral sphingomyelinase inhibitors.a novel sphingomyelin inhibitor ry221b-a, which contains a bipyridyl moiety as a metal coordination site was designed based upon the mechanism of phosphate ester hydrolysis. ry221b-a was synthesized from n-boc-sphingosine in three steps via selective etherification using stannyl acetal. synthesized ry221b-a exhibited relatively-strong inhibitory activity against bc-smase (ic(50)=1.2microm).201020627555
antioxidant and antibacterial activities on foodborne pathogens of artocarpus heterophyllus lam. (moraceae) leaves extracts.total water extract, ethyl acetate, and aqueous fractions from the leaves of artocarpus heterophyllus were evaluated for phenolic content, antioxidant, and antibacterial activities against some foodborne pathogens such as e. coli, listeria monocytogenes, salmonella typhimurium, salmonella enterica, bacillus cereus, enterococcus faecalis, and staphylococcus aureus. the minimum inhibitory concentration (mics) of extract and fractions determined by the agar dilution method were ranged from 221.9 mi ...201020629886
viability and surface properties of spores subjected to a cleaning-in-place procedure: consequences on their ability to contaminate surfaces of equipment.this study was designed to evaluate how conditions encountered by spores during cleaning-in-place (cip) procedures affected their surface properties, their viability and ability to contaminate materials. spores from five bacillus cereus strains were treated with naoh at high temperature. results revealed that high temperatures (exceeding 60 degrees c) and naoh concentrations (over 0.5%) were required to significantly decrease spore viability (3-5log decrease). in these conditions, modifications ...201020630318
specific and reversible immobilization of nadh oxidase on functionalized carbon nanotubes.nanotechnology-inspired biocatalyst systems have attracted a lot of attention in enzyme immobilization recently. theoretically, nanomaterials are ideal supporting materials because they can provide the upper limits on enzyme-efficiency-determining factors such as surface area/volume ratio, enzyme loading capacity and mass transfer resistance. however, common immobilization methods have limited the applicability of these biocatalysts owing to enzyme leaching, 3d structure loss, and strong diffusi ...201020630484
food contamination in fast food restaurants in benin city, edo state, nigeria: implications for food hygiene and safety.to determine the prevalence of food contamination in the fast food restaurants operating in benin city, edo state, nigeria.201020630547
the genome of a bacillus isolate causing anthrax in chimpanzees combines chromosomal properties of b. cereus with b. anthracis virulence plasmids.anthrax is a fatal disease caused by strains of bacillus anthracis. members of this monophyletic species are non motile and are all characterized by the presence of four prophages and a nonsense mutation in the plcr regulator gene. here we report the complete genome sequence of a bacillus strain isolated from a chimpanzee that had died with clinical symptoms of anthrax. unlike classic b. anthracis, this strain was motile and lacked the four prohages and the nonsense mutation. four replicons were ...201020634886
comparing nonsynergistic gamma models with interaction models to predict growth of emetic bacillus cereus when using combinations of ph and individual undissociated acids as growth-limiting factors.a combination of multiple hurdles to limit microbial growth is frequently applied in foods to achieve an overall level of protection. quantification of hurdle technology aims at identifying synergistic or multiplicative effects and is still being developed. the gamma hypothesis states that inhibitory environmental factors aiming at limiting microbial growth rates combine in a multiplicative manner rather than synergistically. its validity was tested here with respect to the use of ph and various ...201020639365
[microbiological contamination of environments and surfaces at commercial restaurants].this study was carried out to provide subsidies for sanitary actions applied to manipulators, environments and surfaces, assessing levels of microbiological contamination in three commercial restaurants (a, b and c) in viçosa, minas gerais state. microbiological analysis were performed for presumptive counting of bacillus cereus and mesophilic aerobic bacteria on surfaces of stainless steel benches, equipments, utensils and hands of the manipulators (swab technique), and air (simple sedimentatio ...201020640321
implication of sphingomyelin/ceramide molar ratio on the biological activity of sphingomyelinase.sphingolipid signaling plays an important, yet not fully understood, role in diverse aspects of cellular life. sphingomyelinase is a major enzyme in these signaling pathways, catalyzing hydrolysis of sphingomyelin to ceramide and phosphocholine. to address the related membrane dynamical structural changes and their feedback to enzyme activity, we have studied the effect of enzymatically generated ceramide in situ on the properties of a well-defined lipid model system. we found a gel-phase format ...201020643068
post-antibacterial effect of thymol.the antibacterial activity of thymol has been well established and reported in the scientific literature. continued suppression of bacterial growth following limited exposure to antimicrobial compounds at different concentrations greater than or equal to the minimum inhibitory concentration level (mic) and at concentrations less than the mic can be used as an indicator of biological activity, and are respectively referred to as a post-antibacterial effect (pae) and a post-antibiotic sub-mic effe ...201020645735
evaluation of the antibacterial activity of the methylene chloride extract of miconia ligustroides, isolated triterpene acids, and ursolic acid derivatives.the methylene chloride extract of miconia ligustroides (dc.) naudin (melastomataceae), the isolated compounds ursolic and oleanolic acids and a mixture of these acids, and ursolic acid derivatives were evaluated against the following microorganisms: bacillus cereus (atcc 14579), vibrio cholerae (atcc 9458), salmonella choleraesuis (atcc 10708), klebsiella pneumoniae (atcc 10031), and streptococcus pneumoniae (atcc 6305). the microdilution method was used for determination of the minimum inhibito ...201020645834
chemical constituents and antimicrobial activities of the essential oils from sedum pallidum var. bithynicum and s. spurium grown in turkey.chemical compositions of the essential oil of sedum pallidum bieb. var. bithynicum (boiss.) and s. spurium bieb. (crassulaceae) from turkey were investigated by gc-ms, and antimicrobial activity of the oil samples were assessed against gram-positive/negative bacteria and yeast-like fungi. thirty-eight and thirty-five components were identified in the essential oils and the main components of these species were found to be caryophyllene oxide from s. pallidum var. bithynicum and hexahydrofarnesyl ...201020645839
an antibiotic, heavy metal resistant and halotolerant bacillus cereus siu1 and its thermoalkaline protease.many workers have reported halotolerant bacteria from saline conditions capable of protease production. however, antibiotic resistance and heavy metal tolerance pattern of such organisms is not documented very well. similarly, only a few researchers have reported the pattern of ph change of fermentation medium during the course of protease production. in this study, we have isolated a halotolerant bacillus cereus siu1 strain from a non-saline environment and studied its antibiotic and heavy meta ...201020646325
bacillus cereus necrotizing fasciitis in a patient with end-stage liver disease.bacillus cereus has been increasingly recognized as a virulent pathogen, particularly in immunocompromised patients.201020649455
the t box regulatory element controlling expression of the class i lysyl-trna synthetase of bacillus cereus strain 14579 is functional and can be partially induced by reduced charging of asparaginyl-trnaasn.lysyl-trna synthetase (lysrs) is unique within the aminoacyl-trna synthetase family in that both class i (lysrs1) and class ii (lysrs2) enzymes exist. lysrs1 enzymes are found in archaebacteria and some eubacteria while all other organisms have lysrs2 enzymes. all sequenced strains of bacillus cereus (except ah820) and bacillus thuringiensis however encode both a class i and a class ii lysrs. the lysk gene (encoding lysrs1) of b. cereus strain 14579 has an associated t box element, the first rep ...201020649968
hypercat: an extension of the supercat database for global multi-scheme and multi-datatype phylogenetic analysis of the bacillus cereus group population.the bacillus cereus group of bacteria includes species that are of significant medical and economic importance. we previously developed the supercat database, which integrates data from all five multilocus sequence typing (mlst) schemes available to infer the genetic relatedness within this group. since large numbers of isolates have been typed by other techniques, these should be incorporated in order to provide the most comprehensive and truly global view of the b. cereus group population. the ...201020651034
humidity-dependent bacterial cells functional morphometry investigations using atomic force microscope.the effect of a relative humidity (rh) in a range of 93-65% on morphological and elastic properties of bacillus cereus and escherichia coli cells was evaluated using atomic force microscopy. it is shown that gradual dehumidification of bacteria environment has no significant effect on cell dimensional features and considerably decreases them only at 65% rh. the increasing of the bacteria cell wall roughness and elasticity occurs at the same time. observed changes indicate that morphological prop ...201020652040
spectral, thermal and in vitro antimicrobial studies of cyclohexylamine-n-dithiocarbamate transition metal complexes.transition metal complexes of the type [m(l)(2)] and those containing monodentate phosphines of the type [m(l)(2)(pph(3))] {m=ni, co, cu and zn; l=cyclohexylamine-n-dithiocarbamate; pph(3)=triphenylphosphine} have been synthesized. the complexes were characterized using ir, uv-vis, nmr spectroscopy, and thermal analysis (tga). the (1)h nmr, (13)c nmr and (31)p nmr showed the expected signals for the dithiocarbamate and triphenylphosphine moieties. the spectral studies in all compounds revealed t ...201020655805
the chemical constituents of endophytic fungus trichoderma sp. mff-1.a fungal strain named mff-1 was isolated from the flower of pyrethrum cinerariifolium. based on the sequence at the internal transcribed spacer (its) region, this strain was identified as a trichoderma sp. two new compounds, including a mitorubrin derivative and its potential biogenetic precursor, together with a known compound, were isolated from the cultures of the endophytic fungus. their structures were established by spectroscopic methods and determined to be (3s*,6r*,7r*)-3,4,5,6,7,8-hexah ...201020658667
anthrax spore detection by a luminex assay based on monoclonal antibodies that recognize anthrose-containing oligosaccharides.the similarity of endospore surface antigens between bacteria of the bacillus cereus group complicates the development of selective antibody-based anthrax detection systems. the surface of b. anthracis endospores exposes a tetrasaccharide containing the monosaccharide anthrose. anti-tetrasaccharide monoclonal antibodies (mabs) and anti-anthrose-rhamnose disaccharide mabs were produced and tested for their fine specificities in a direct spore enzyme-linked immunosorbent assay (elisa) with inactiv ...201020660139
ability of bacillus cereus group strains to cause food poisoning varies according to phylogenetic affiliation (groups i to vii) rather than species affiliation.cytotoxic activity levels of culture filtrates and toxin distributions varied according to the phylogenetic group (i to vii) within the bacillus cereus group, suggesting that these groups are of different clinical significance and are more suitable than species affiliations for determining food poisoning risk. a first-line, simple online tool (https://www.tools.symprevius.org/bcereus/english.php) to assign strains to the different phylogenetic groups is presented.201020660215
an evaluation of commercial dna extraction kits for the isolation of bacterial spore dna from soil.to evaluate six commercial dna extraction kits for their ability to isolate pcr-quality dna from bacillus spores in various soil samples.201020666869
evaluation of various pcr assays for the detection of emetic toxin producing bacillus cereus.because conventional methods for detecting emetic-toxin-producing b. cereus are laborious and costly, various pcr assays, which are easy and cheap, have recently been reported. therefore, this study estimated and compared the ability of various pcr assays to detect emetic-toxin-producing b. cereus strains isolated in korea. the pcr assays were performed on 160 b. cereus strains, including 40 emetic-toxin-producing strains. although the species-specific pcr assays were all shown to be highly spec ...201020668404
inactivation of food-borne spoilage and pathogenic micro-organisms on the surface of a photoactive polymer.the photodynamic action of a novel photoactive polymer comprising covalently bound anthraquinone (aq) moieties was evaluated after developing a methodology to reliably immobilize viable micro-organisms onto polymer film surfaces. the survival of escherichia coli, bacillus cereus (vegetative cells and spores), fusarium oxysporum and saccharomyces cerevisiae microbes inoculated on the surface of inert polymeric substrates was assessed to determine the effect of inoculum composition, drying rate an ...201020670362
survey on the microbiological quality of chinese food preparations.the microbiological quality of 118 samples of traditional chinese food from restaurants and take-away establishments in the provinces of padua, treviso, and venice was examined (april-july, 2008). these food items included various ready-to-eat products. the microbiological quality of the majority of these food items was acceptable; only 12% of samples had values greater than or equal to 6 log cfu/g (eight items were salads) and 19% of samples had values greater than 500 cfu fecal coliforms/g (p ...201020490663
lmbe proteins from bacillus cereus are de-n-acetylases with broad substrate specificity and are highly similar to proteins in bacillus anthracis.the genomes of bacillus cereus and its closest relative bacillus anthracis each contain two lmbe protein family homologs: bc1534 (ba1557) and bc3461 (ba3524). only a few members of this family have been biochemically characterized including n-acetylglucosaminylphosphatidyl inositol (glcnac-pi), 1-d-myo-inosityl-2-acetamido-2-deoxy-alpha-d-glucopyranoside (glcnac-ins), n,n'-diacetylchitobiose (glcnac(2)) and lipoglycopeptide antibiotic de-n-acetylases. all these enzymes share a common feature in ...201020491912
characterization of the properties of pediococcus parvulus for probiotic or protective culture use.pediococcus parvulus 2.6 (previously pediococcus damnosus 2.6, here confirmed as p. parvulus by 16s dna sequencing) displayed antibacterial activity toward several bacterial species, including isolates found as contaminants in oats, herein genetically identified as bacillus cereus. no inhibition of listeria monocytogenes was found under the conditions used. antibacterial activity was retrieved after ammonium sulfate or acetone precipitation showed it to be peptide mediated. p. parvulus 2.6 has p ...201020501049
blechnum orientale linn - a fern with potential as antioxidant, anticancer and antibacterial agent.blechnum orientale linn. (blechnaceae) is used ethnomedicinally for the treatment of various skin diseases, stomach pain, urinary bladder complaints and sterilization of women. the aim of the study was to evaluate antioxidant, anticancer and antibacterial activity of five solvent fractions obtained from the methanol extract of the leaves of blechnum orientale linn.201020429956
[screening of collagenase-producing strain and purification of bacillus cereus collagenase].we isolated the strain mbl13 with high collagenase productivity from the soil of piled up animal bones. it was identified as bacillus cereus. we purified and characterized bacillus cereus collagenase (bcc). the molecular weight of bcc was 38.0 kda and the optimum temperature and ph for the enzyme activity were 40 degrees c and 8.0 respectively. the enzyme was stable when the temperature was below 50 degrees c, but only retained 10% activity when kept at 60 degrees c for 1 h. the enzyme activity ...201020432938
studies of the commitment step in the germination of spores of bacillus species.spores of bacillus species are said to be committed when they continue through nutrient germination even when germinants are removed or their binding to spores' nutrient germinant receptors (grs) is both reversed and inhibited. measurement of commitment and the subsequent release of dipicolinic acid (dpa) during nutrient germination of spores of bacillus cereus and bacillus subtilis showed that heat activation, increased nutrient germinant concentrations, and higher average levels of grs/spore s ...201020435722
food poisoning associated with emetic-type of bacillus cereus in korea.bacillus cereus can cause diarrheal and emetic types of food poisoning but little study has been done on emetic type of food poisoning in korea. the objective of this study was to report on the emetic type of food poisoning associated with b. cereus in korea. the toxin gene profile, toxin production, and antibiotic resistance of b. cereus isolates were investigated in this study. b. cereus was detected in three out of four samples, while the other food poisoning bacteria were not detected. all i ...201020446859
novel 6,8-dibromo-4(3h)quinazolinone derivatives of anti-bacterial and anti-fungal activities.starting from 4-(6,8-dibromo-2-phenyl-4-oxo-(4h)-quinazolin-3-yl)-benzoic acid ethyl ester (ii) and its acid hydrazide iii, a new series of schiff bases iv and their cyclized products, thiazolidinones v, oxadiazole viii, pyrazoles x-xii, pyrroles xiii-xv and other related products were synthesized. these compounds were screened for their anti-bacterial activity against gram-positive bacteria (staphylococcus aureus, legionella monocytogenes and bacillus cereus) and gram-negative bacteria (escheri ...201020452707
quantification of the effect of culturing temperature on salt-induced heat resistance of bacillus species.short- and long-term exposure to mild stress conditions can activate stress adaptation mechanisms in pathogens, resulting in a protective effect toward otherwise lethal stresses. the mesophilic strains bacillus cereus atcc 14579 and atcc 10987 and the psychrotolerant strain b. weihenstephanensis kbab4 were cultured at 12 degrees c and 30 degrees c until the exponential growth phase (i) in the absence of salt, (ii) in the presence of salt, and (iii) with salt shock after they reached the exponent ...201020453152
the characterization and diversity of bacterial endophytes of grapevine.the diversity of culturable and nonculturable bacterial endophytes of grapevine (vitis vinifera l.) was examined using a combination of cultivation and molecular methods. entire grapevines were sampled to characterize bacterial diversity from different locations throughout the vine. gas chromatography of fatty acid methyl esters (fames) was used to identify culturable isolates prior to subsequent further microbiological characterization, whilst denaturing gradient gel electrophoresis (dgge) was ...201020453907
manipulation of thiocillin variants by prepeptide gene replacement: structure, conformation, and activity of heterocycle substitution mutants.bacillus cereus atcc 14579 converts the c-terminal 14 residues of a 52-mer prepeptide into a related set of eight variants of the thiocillin subclass of thiazolyl peptide antibiotics by a cascade of post-translational modifications that alter 13 of those 14 residues. we have introduced prepeptide gene variants into a knockout strain to conduct an alanine scan of all 14 progenitor residues, as well as a serine scan of the six cysteine residues that are converted to thiazoles in the mature natural ...201020455532
antioxidant, antibacterial and antigiardial activities of walsura robusta roxb.walsura robusta roxb. (family: meliaceae) is a well-known multi-purpose medicinal plant, and has been employed for a wide range of disease conditions without documented scientific data. in the current study, four pure isolated compounds, 3,4,5-trimethoxyphenyl beta-d-glucopyranoside (1), turpinionoside a (2), (+)-lyoniresinol 3alpha-o-beta-d-glucopyranoside (3) and (-)-lyoniresinol 3alpha-o-beta-d-glucopyranoside (4), were isolated from the leaves and twigs of w. robusta. biological evaluation f ...201020461627
siderophore-mediated iron acquisition in bacillus anthracis and related strains.recent observations have shed light on some of the endogenous iron-acquisition mechanisms of members of the bacillus cereus sensu lato group. in particular, pathogens in the b. cereus group use siderophores with both unique chemical structures and biological roles. this review will focus on recent discoveries in siderophore biosynthesis and biology in this group, which contains numerous human pathogens, most notably the causative agent of anthrax, bacillus anthracis.201020466767
antimicrobial activity and essential oil composition of a new t. argyrophyllum (c. koch) tvzel var. argyrophyllum chemotype.water-distilled essential oils from flowers and stems of tanacetum argyrophyllum var. argyrophyllum from turkey were analyzed by gc and gc/ms. the flower oil of t. argyrophyllum var. argyrophyllum was characterized with camphor 29.7%, borneol 12.0%, 1,8-cineole 8.4% and bornyl acetate 6.1%. stem oil was characterized with camphor 26.6%, 1,8-cineole 17.5%, and borneol 15.0%. our previous research and literature on the essential oil of this plant reported oils with high thujone content unlike the ...201020484836
rapid multi-locus sequence typing using microfluidic biochips.multiple locus sequence typing (mlst) has become a central genotyping strategy for analysis of bacterial populations. the scheme involves de novo sequencing of 6-8 housekeeping loci to assign unique sequence types. in this work we adapted mlst to a rapid microfluidics platform in order to enhance speed and reduce laboratory labor time.201020485679
polymyxin-direct hemoperfusion for sepsis-induced multiple organ failure.we report a case of multiple organ failure caused by the bacillus cereus infection during acute lymphoblastic leukemia therapy, who was treated successfully. a 15-year-old male developed (b. cereus) sepsis on the 19th day after chemotherapy initiation. polymyxin-direct hemoperfusion for septic shock was started, followed by continuous hemodiafiltration. his condition improved after starting the hemoperfusion. at the onset of sepsis, elevated levels of serum inflammatory cytokines, anti-inflammat ...201020486188
environmental factors determining the epidemiology and population genetic structure of the bacillus cereus group in the field.bacillus thuringiensis (bt) and its insecticidal toxins are widely exploited in microbial biopesticides and genetically modified crops. its population biology is, however, poorly understood. important issues for the safe, sustainable exploitation of bt include understanding how selection maintains expression of insecticidal toxins in nature, whether entomopathogenic bt is ecologically distinct from related human pathogens in the bacillus cereus group, and how the use of microbial pesticides alte ...201020502683
rapid analysis of gram-positive bacteria in water via membrane filtration coupled with nanoprobe-based maldi-ms.matrix-assisted laser desorption/ionization (maldi) mass spectrometry (ms) is challenging when it is directly applied to identify bacteria in water. this study demonstrates a rapid, sensitive, and selective technique for detection of gram-positive bacteria in water. it involves a combination of membrane filtration (mf) and vancomycin-conjugated magnetite nanoparticles (vnps) to selectively separate and concentrate gram-positive bacteria in tap water and reservoir water, followed by rapid analysi ...201020512317
essential oil composition and antibacterial activity of tanacetum argenteum (lam.) willd. ssp. argenteum and t. densum (lab.) schultz bip. ssp. amani heywood from turkey.water-distilled essential oils from aerial parts of tanacetum argenteum ssp. argenteum and t. densum ssp. amani from turkey were analyzed by gc and gc/ms. the essential oil of t. argenteum ssp. argenteum was characterized with alpha-pinene 36.7%, beta-pinene 27.5% and 1,8-cineole 9.8%. t. densum ssp. amani was characterized with beta-pinene 27.2%, 1,8-cineole 13.1%, alpha-pinene 9.7% and p-cymene 8.9%. antibacterial activity of the oils were evaluated for five gram-positive and five gram-negativ ...201020513969
[bacillus cereus keratitis--case report].bacillus cereus is a gram-positive, rod-shaped, beta hemolytic bacterium which rarely causes eye infections. we present a case of 15 year old boy with unilateral keratitis. he was treated for two months by his ophthalmologist who suspected herpetic keratitis. patient most probably suffered secondary infection by bacillus cereus. after bacillus was identified and targeted treatment given clinical picture improved rapidly. until now there are only few cases of bacillus cereus keratitis published.201020521509
composition and antimicrobial activity of the essential oils from flowers of senecio othonnae, s. racemosus, and s. nemorensis.the chemical composition of the essential oils of senecio othonnae, s. racemosus, and s. nemorensis obtained by hydrodistillation of fresh flowers were analysed by gc-fid and gc-ms. a total of seventy-six compounds were identified, constituting over 83.1%, 97.7% and 86.8% of the total oil composition of the flowers of s. othonnae, s. racemosus, and s. nemorensis. the main components of the oils were caryophyllene oxide (18.6%), (e)-beta-farnesene (21.6%), and gamma-curcumene (42.8%), respectivel ...201020521557
biosynthesis of a new udp-sugar, udp-2-acetamido-2-deoxyxylose, in the human pathogen bacillus cereus subspecies cytotoxis nvh 391-98.we have identified an operon and characterized the functions of two genes from the severe food-poisoning bacterium, bacillus cereus subsp. cytotoxis nvh 391-98, that are involved in the synthesis of a unique udp-sugar, udp-2-acetamido-2-deoxyxylose (udp-n-acetyl-xylosamine, udp-xylnac). uglcnacdh encodes a udp-n-acetyl-glucosamine 6-dehydrogenase, converting udp-n-acetylglucosamine (udp-glcnac) to udp-n-acetyl-glucosaminuronic acid (udp-glcnaca). the second gene in the operon, uxnacs, encodes a ...201020529859
an organic solvent-stable protease from organic solvent-tolerant bacillus cereus wq9-2: purification, biochemical properties, and potential application in peptide synthesis.an extracellular solvent-stable protease producing bacterium wq9-2 was isolated and identified taxonomically as bacillus cereus. the protease from strain wq9-2 was purified to homogeneity with an estimated molecular mass of 37kda. the purified protease showed maximum activity at 50 degrees c and ph 8.0. the protease may be classified as a metalloprotease since it was strongly inhibited by edta and 1,10-phenanthroline. the protease showed extreme activity and stability in the presence of both 50% ...201020541397
dissemination of bacillus cereus in the burn unit due to contaminated non-sterile gloves. 201020542594
a novel spore protein, exsm, regulates formation of the exosporium in bacillus cereus and bacillus anthracis and affects spore size and shape.bacillus cereus spores are assembled with a series of concentric layers that protect them from a wide range of environmental stresses. the outermost layer, or exosporium, is a bag-like structure that interacts with the environment and is composed of more than 20 proteins and glycoproteins. here, we identified a new spore protein, exsm, from a beta-mercaptoethanol extract of b. cereus atcc 4342 spores. subcellular localization of an exsm-green fluorescent protein (gfp) protein revealed a dynamic ...201020543075
isolation and identification of bacillus spp. and related genera from different starchy foods.samples of butternut squash, potatoes, rice, and wheat flour were analyzed. bacillus spp. and related species belonging to paenibacillus and brevibacillus genera were found in 96% of the samples. in butternut squash, predominant species were bacillus pumilus and paenibacillus polymyxa together with other bacillus spp. species (b. cereus, b. licheniformis, b. sphaericus, and b. subtilis). in all the potato samples, bacillus species were detected (b. cereus, b. mycoides, and b. licheniformis). als ...201020546413
microbial growth in dry grain food (sunsik) beverages prepared with water, milk, soymilk, or honey-water.this study was conducted to investigate the growth of microorganisms, including pathogenic bacteria such as cronobacter sakazakii and bacillus cereus, in sunsik beverages made of water, milk, soymilk, or honey-water during storage at room temperature. prepared sunsik beverages were stored at room temperature and the growth of total aerobic counts, escherichia coli/coliforms, and yeast and mold were measured. also, samples inoculated with a cocktail of c. sakazakii or b. cereus spores were stored ...201020546416
isolation and characterization of a novel analyte from bacillus subtilis sc-8 antagonistic to bacillus cereus.in this study, an effective substance was isolated from bacillus subtilis sc-8, which was obtained from traditionally fermented soybean paste, cheonggukjang. the substance was purified by hplc, and its properties were analyzed. it had an adequate antagonistic effect on bacilluscereus, and its spectrum of activity was narrow. when tested on several gram-negative and gram-positive foodborne pathogenic bacteria such as salmonella enterica, salmonella enteritidis, staphylococcus aureus, and listeria ...201020547349
development of starter culture for improved processing of lafun, an african fermented cassava food product.to select appropriate micro-organisms to be used as starter culture for reliable and reproducible fermentation of lafun.201020553347
novel family of antimicrobial peptides from the skin of rana shuchinae.so far numerous antimicrobial peptides have been characterized from amphibians. in this work, a new family of antimicrobial peptides, named shuchin, was purified and characterized from skin secretions of the frog, rana shuchinae that lives in freezing mountains. totally two members of shuchin (shuchin 1 and 2) were identified with the amino acid sequence of nalsmprnkcnralmcfg and nalssprnkcdrasscfg, respectively. cdnas encoding shuchins were cloned from the skin cdna library of r. shuchinae. the ...201020553780
genome-wide single nucleotide polymorphism typing method for identification of bacillus anthracis species and strains among b. cereus group species.as an issue of biosecurity, species-specific genetic markers have been well characterized. however, bacillus anthracis strain-specific information is currently not sufficient for traceability to identify the origin of the strain. by using genome-wide screening using short read mapping, we identified strain-specific single nucleotide polymorphisms (snps) among b. anthracis strains including japanese isolates, and we further developed a simplified 80-tag snp typing method for the primary investiga ...201020554827
inactivation of bacillus cereus by na-chlorophyllin-based photosensitization on the surface of packaging.this study was focused on the possibility to inactivate food-borne pathogen bacillus cereus by na-chlorophyllin (na-chl)-based photosensitization in vitro and after attachment to the surface of packaging material.201020557405
generation of chitin-derived oligosaccharides toxic to pathogenic bacteria using chia74, an endochitinase native to bacillus thuringiensis.to demonstrate that an endochitinase (chia74) native to bacillus thuringiensis can be used to generate chitin-derived oligosaccharides (ogs) with antibacterial activity against a number of aetiological agents of disease, including bacteria that cause diarrhoeal and emetic syndromes in humans.201020557451
an independent in vitro comparison of povidone iodine and sterilid.povidone iodine (pi) and sterilid (sl) are biocides used to decrease bacterial load on the eyelid margin. we compared the antibacterial activity of pi and sl to determine the better antiseptic.201020565315
growth of microorganisms in total parenteral nutrition solutions containing lipid.to identify the microorganisms that can grow rapidly in total parenteral nutrition (tpn) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (staphylococcus aureus, serratia marcescens, bacillus cereus, and candida albicans) in tpn solutions containing lipid.201020567610
cereulide and valinomycin, two important natural dodecadepsipeptides with ionophoretic activities.cereulide produced by bacillus cereus sensu stricto and valinomycin synthesized mainly by streptomyces spp. are natural dodecadepsipeptide ionophores that act as potassium transporters. moreover, they comprise three repetitions of similar tetrapeptide motifs synthesized by nonribosomal peptide synthesis complexes. resemblances in their structure find their reflections in the same way of action. the toxicity of valinomycin and cereulide is an effect of the disturbance of ionic equilibrium and tra ...201020568524
response of bacillus cereus to gamma-irradiation in combination with carvacrol or mild heat treatment.carvacrol and mild heat treatment were tested for their efficiency to increase the radiosensitivity of bacillus cereus in broth. the bacterium was treated with gamma-irradiation alone or in combination with carvacrol at its minimal inhibitory concentration or mild heat treatment for 10 min at 45 degrees c. the effects of this combination of treatments were studied on various parameters: the bacterial viability, the modifications of the cell morphology with scanning electron microscopy (sem), the ...201020568774
cell-based biosensor for rapid screening of pathogens and toxins.development and validation of a mammalian cell-based biosensor for application in food defense and food safety was investigated. three prototypes of the biosensor capable of handling different sample types were developed and tested with food and beverages. the sensing element is a b lymphocyte ped-2e9 cell-line, encapsulated in collagen matrix in 3d scaffold. the uniqueness of this biosensor is that it detects analyte interaction with mammalian cells and is able to distinguish pathogenic from no ...201020570502
modelling the influence of palmitic, palmitoleic, stearic and oleic acids on apparent heat resistance of spores of bacillus cereus ntcc 11145 and clostridium sporogenes pasteur 79.3.heat resistance of spores is affected by many factors such as temperature, ph, water activity (aw) and others. previous studies have reported that free fatty acids can affect the germination and growth of bacterial spores. in this study, we investigated the influence of free fatty acids in heating medium or in recovery medium on the heat resistance of spores of bacillus cereus ntcc 11145 and clostridium sporogenes pasteur 79.3. four free fatty acids were studied: palmitic, palmitoleic, stearic a ...201020573415
hydrolysis of organophosphate esters: phosphotriesterase activity of metallo-beta-lactamase and its functional mimics.the phosphotriesterase (pte) activity of a series of binuclear and mononuclear zinc(ii) complexes and metallo-beta-lactamase (mbetal) from bacillus cereus was studied. the binuclear complex 1, which exhibits good mbetal activity, shows poor pte activity. in contrast, complex 2, a poor mimic of mbetal, exhibits much higher activity than 1. the replacement of cl(-) ligands by oh(-) is important for the high pte activity of complex 2 because this complex does not show any catalytic activity in meth ...201020575118
antioxidant and antimicrobial activity of alpinia officinarum.alpinia officinarum is a rhizome belonging to the family zingeberaeceae. hydro alcoholic extract by hot and cold maceration and methanol extract by percolation process qualitative phytochemical analysis of extract of alpinia officinarum rhizome showed a majority of the compound including tannins, alkaloids, flavonoids and saponins. hydroalcoholic extract prepared by hot maceration process was found to contain more phenol and flavonol and it was measured as 50.1 mg/g and 54.02 mg/g, respectively. ...201020582210
a new prenylated flavanoid with antibacterial activity from propolis collected in egypt.a novel prenylated flavanoid, isonymphaeol-d (1), together with two known compounds, isonymphaeol-b (2) and nymphaeol-b (3), were isolated from egyptian propolis. the structures of the isolated compounds were determined by various spectroscopic methods. 1 exhibited antibacterial activity against gram-positive (bacillus cereus, staphylococcus aureus) and gram-negative strains (serratia sp., pseudomonos sp., escherichia coli).201020184018
identification and characterization of microorganisms from earthworm viscera for the conversion of fish wastes into liquid fertilizer.five bacteria isolated from earthworm viscera and identified as brevibacillus agri, bacillus cereus, bacillus licheniformis, and brevibacillus parabrevis by 16s rrna sequencing were employed in the conversion of fish wastes generated from a restaurant specializing in sliced raw fish into fertilizer. within 120h after inoculation of autoclaved fish waste with 5.15 x 10(5) cfu ml(-1) mixed isolates, the amount of dry sludge decreased from 29.4 to 0.2g, the ph changed from 7.05 to 5.70, and the cel ...201020189380
zwittermicin a: synthesis of analogs and structure-activity studies.analogs and diastereomers of the natural product zwittermicin a were prepared. sar studies of these compounds reveal the antifungal activity to be dependent singularly upon the natural constitution and configuration.201020189808
identification of bacillus cereus genes specifically expressed during growth at low temperatures.the mechanisms involved in the ability of bacillus cereus to multiply at low temperatures were investigated. it was assumed that many genes involved in cold acclimation would be upregulated at low temperatures. recombinase-based in vivo expression technology (ivet) was adapted to the detection of the transient activation of b. cereus promoters during growth at 10 degrees c. four independent screenings of a promoter library from type strain atcc 14579 were performed, and 17 clones were isolated. ...201020190083
rapid detoxification of cereulide in bacillus cereus food poisoning.bacillus cereus is recognized as a major pathogenic bacterium that causes food poisoning and produces gastrointestinal diseases of 2 types: emetic and diarrheal. the emetic type, which is often linked to pasta and rice, arises from a preformed toxin, cereulide, in food. rapid and accurate diagnostic methods for this emetic toxin are important but are limited. here we describe 3 patients with b cereus food poisoning in which cereulide was detected and measured sequentially. three family members b ...201020194285
using enzymes to remove biofilms of bacterial isolates sampled in the food-industry.the aim of this study was to analyze the cleaning efficiency of polysaccharidases and proteolytic enzymes against biofilms of bacterial species found in food industry processing lines and to study enzyme effects on the composition of extracellular polymeric substances (eps) and biofilm removal in a clean-in-place (cip) procedure. the screening of 7 proteases and polysaccharidases for removal of biofilms of 16 bacterial species was first evaluated using a microtiter plate assay. the alkaline ph b ...201020198521
monoclonal antibodies against recombinant hemolysin bl complex of bacillus cereus.a three component complex system, designated hemolysin bl (hbl), is believed to be the major diarrheal toxin of bacillus cereus. identification of hbl toxin by immunoassay is advantageous over pcr as it detects the expressed form of the gene, thereby differentiating pathogenic strains from nonpathogenic strains. however, most of the immunoassays, like the bcet rpla kit, are based on the utilization of polyclonal antisera, which show cross-reactivity at times with other bacillus species. the use ...201020199155
synergistic effects of ethanol and uv radiation to reduce levels of selected foodborne pathogenic bacteria.the purpose of this study was to determine whether combined treatments would produce synergistic disinfection effects on food products during food processing compared with single treatments. we investigated the bactericidal effects of a commercial chemical disinfectant (ethanol) and of uv radiation on bacillus cereus f4810/72, cronobacter sakazakii kctc 2949, staphylococcus aureus atcc 35556, escherichia coli atcc 10536, and salmonella enterica typhimurium no/na in vitro. various concentrations ...201020202345
hemolysis induced by bacillus cereus sphingomyelinase.bacillus cereus sphingomyelinase (bc-smase) induces hemolysis of sheep erythrocytes which contain large amounts of sphingomyelin. we investigated the mechanism of this hemolysis in comparison to that induced by clostridium perfringens alpha-toxin. pertussis toxin, a gi-specific inhibitor, n-oleoylethernolamine, a ceramidase inhibitor, and dihydrosphingosine, a sphingosine kinase inhibitor, did not inhibit the hemolysis by bc-smase, but did inhibit that by alpha-toxin. bc-smase broadly bound to w ...201020214877
phenol degradation by bacillus cereus: pathway and kinetic modeling.the microbial degradation of phenol by pure cultures bacillus cereus mtcc 9817 strain akg1 and b. cereus mtcc 9818 strain akg2 is studied in batch mode for several initial concentrations of phenol in the range of 100-2000 mg/l with an interval of 100mg/l. degradation pathways are investigated at initial phenol concentrations of 100, 500, 1000, 1500, and 2000 mg/l. the bacteria are able to degrade phenol of concentration as high as 2000 mg/l. the maximum degradation rate is obtained at an initial ...201020219360
structural investigation of transcriptional regulator hlyiir: influence of a disordered region on protein fold and dimerization.b. cereus hlyiir belongs to the tetr family of dimeric transcriptional regulators. unlike other members of the tetr family, hlyiir contains an insert between alpha-helices alpha8 and alpha9, which is located at the subunit-subunit interface. n-terminal segment of this insert (amino acids, pro161-ser169) forms a short alpha-helix alpha8* that occupies a complementary cavity on the surface of the adjacent subunit, whereas the c-terminal segment comprising 16 amino acids (leu170-glu185) is disorder ...201020225260
persistence strategies of bacillus cereus spores isolated from dairy silo tanks.survival of bacillus cereus spores of dairy silo tank origin was investigated under conditions simulating those in operational dairy silos. twenty-three strains were selected to represent all b. cereus isolates (n = 457) with genotypes (rapd-pcr) that frequently colonised the silo tanks of at least two of the sampled eight dairies. the spores were studied for survival when immersed in liquids used for cleaning-in-place (1.0% sodium hydroxide at ph 13.1, 75 degrees c; 0.9% nitric acid at ph 0.8, ...201020227599
the prevalence and concentration of bacillus cereus in retail food products in brisbane, australia.illness associated with bacillus cereus may be underreported as very few of those affected seek medical attention owing to the mild nature and short duration of symptoms. for this reason there is little information on the prevalence and concentration of this pathogen in retail food products. a total of 1263 retail food samples were examined for b. cereus using the australian standard 1766.2.6 (1991): spread plate technique on polymyxin pyruvate egg yolk mannitol bromothymol blue agar, of which t ...201020230270
cwpfm (entfm) is a bacillus cereus potential cell wall peptidase implicated in adhesion, biofilm formation, and virulence.bacillus cereus entfm displays an nlpc/p60 domain, characteristic of cell wall peptidases. the protein is involved in bacterial shape, motility, adhesion to epithelial cells, biofilm formation, vacuolization of macrophages, and virulence. these data provide new information on this, so far, poorly studied toxin and suggest that this protein is a cell wall peptidase, which we propose to rename cwpfm.201020233921
[influence of permafrost microorganisms on the quality and duration of life of laboratory animals].microorganisms of genus bacillus strain 3m (permafrost microorganisms--pm), allocated from the samples of permafrost soils from mamontova mountain (yakutia) are influenced by the quality and duration of life of elderly mice. pms were injected intraperitoneally to cba mice at the age of 17 months. motion activity, muscular force (lifting of cargo test), food instinct activity (dosed starvation test) and cellular immunity activity (reaction of delayed-type hypersensitivity in vivo) of animals have ...201020297696
mechanism of killing of spores of bacillus cereus and bacillus megaterium by wet heat.to determine the mechanism of wet heat killing of spores of bacillus cereus and bacillus megaterium.201020302598
differentiation between probiotic and wild-type bacillus cereus isolates by antibiotic susceptibility test and fourier transform infrared spectroscopy (ft-ir).animal feed often contains probiotic bacillus strains used as feed additives. spores of the non-pathogenic b. cereus var. toyoi (product name toyocerin) are used. distinguishing between toxic wild-type bacillus cereus strains and this probiotic strain is essential for evaluating the quality and risk of feed. bacillus cereus cip 5832 (product name paciflor was used as probiotic strain until 2001. the properties of the two probiotic strains are quite similar. differentiating between probiotic stra ...201020303194
bisphenol a removal by the dracaena plant and the role of plant-associating bacteria.dracaena sanderiana and dracaena fragrans plants, as representatives of native, tropical, evergreen plants with fibrous root systems, were evaluated for bisphenol a (bpa) tolerance and uptake capability. d. sanderiana demonstrated significantly higher bpa removal capability than d. fragrans. therefore, it was chosen for further study. d. sanderiana tolerated bpa toxicity levels up to 80 microm, while higher bpa concentrations damaged the plant. in the sterile hydroponic system with an initial bp ...201020304555
an assessment of the antibacterial activity in larval excretion/secretion of four species of insects recorded in association with corpses, using lucilia sericata meigen as the marker species.the relative antibacterial activities of excretion/secretion (es) from two carrion-feeding insects, calliphora vicina robineau-desvoidy and dermestes maculatus degeer, and a detritivore, tenebrio molitor linnaeus, were compared to that of lucilia sericata meigen, a species with es of known antibacterial capacity, in order to explore the antimicrobial potential of other carrion and detritivore species. viable counts were used to assess time-kill of es against five bacterial species, staphylococcu ...201020307342
[preparation of a 96-microwell plate dna diagnostic chip for detection of foodborne bacteria and its application in an incident of food poisoning].to develop a 96-microwell plate dna diagnostic chip for simultaneous detection of 9 major foodborne bacteria.201020335099
antimicrobial, cytotoxicity and phytochemical screening of jordanian plants used in traditional medicine.antimicrobial activity and cytotoxicity of fifty one extracts of different parts of 14 plants were studied. ethanol, methanol, aqueous, butanol, and n-hexane extracts were tested against three gram negative, two gram positive bacteria, and two fungi. cytotoxicity and phytochemical screening were determined using mtt and tlc assays, respectively. of the fifty one extracts, twenty two showed activities against different microorganisms with mics ranging from 62.5 to 1000 microg/ml. the highest acti ...201020336015
expression of alkaline proteinase gene in two recombinant bacillus cereus feather-degrading strains.two bacillus cereus feather-degrading strains (23/1 and 6/2) were transformed using a recombinant plasmid p5.2 carrying the alkaline proteinase gene (apre). a high level of the apre gene expression was observed when the recombinant strains were grown on sporulation medium. the expression of the apre gene proceeded during the early stationary phase and the p5.2 plasmid was segregationally and structurally stable in both strains. the two recombinant strains grown on a mineral medium with 1 % chick ...201020336500
Refresh
firstprev
...21222324252627282930...
nextlast
Displaying items 2301 - 2400 of 8187