Publications

body mass index variations: centiles from birth to 87 years.	this report provides body mass index (weight/height2) values for the french population from birth to the age of 87 years. bmi curves increase during the first year, decrease until the age of 6, increase again up to 65 years and decrease thereafter. these variations reflect the total changes of fat body mass during life. the 50th centile values of wt/ht2 at the ages of 20, 40, 60, 80 years are 21.5, 24.6, 25.4, 24.4 kg/m2 for men and 20.6, 22.6, 24.1, 23.4 kg/m2 for women. the values for the 3rd, ...	2007	1855495
lightness, embeddedness, and contrast.		2007	5054071
new prosthetic venous collar anastomotic technique: combining the best of other procedures.		2007	1913097
[lifestyle and reproductive situations of adolescent girls in large cities].	an analysis is made of a sociological interview survey of teenage girls residing and attending educational establishments in one of the moscow regions. teenage girls' views on some issues of future family life have been identified. it has been found that there is a wide prevalence of harmful habits among these populations, the majority of girls have a sexual experience along with low culture of sexual behavior and healthy lifestyle habits.	2007	1838436
[the obstetric risk in adolescent primiparous females].	the obstetric risks in primiparae between 12 and 17 years of age have been reviewed in cases treated at our hospital from 1982 to 1987. we looked for intrauterine foetal deaths, premature deliveries (birth weight less than 2500 g), breech presentations, preeclampsia, caesarean sectio rate, forceps rate, and thirdstage complications. the risks found in the group of 476 young primiparae were compared to those amongst the 26,768 overall deliveries during the same period. the incidence of breech pre ...	2007	2737441
systemic or local treatment of erythrasma? a comparison between erythromycin tablets and fucidin cream in general practice.	in a danish multi-practice study the efficacy of erythromycin tablets (abboticin 500 mg tablets), fusidic acid cream (fucidin cream), and placebo was compared in 86 patients (71 men and 15 women) with erythrasma. the patients were treated 'double-blind' for 14 days with either active tablets + placebo cream, placebo tablets + active cream, or placebo tablets + placebo cream. the signs of erythrasma, i.e. colour intensity, demarcation, and scaling of the affected area, as well as degree of fluore ...	2007	2041927
in the aftermath: health care in kuwait city.		2007	1865865
cognitive and affective disorders in elderly diabetics.	in non-insulin-dependent diabetes mellitus, performance of complex cognitive tasks requiring the storage and retrieval of new information is poorer than in age-matched controls. by contrast, performance of less demanding tasks such as immediate memory and simple reaction time is essentially equivalent for niddm patients and controls. this pattern parallels the cognitive change observed with normal aging, in which age differences are minimal on less demanding immediate memory tasks but older adul ...	2007	2224744
[monitoring control of the visual evoked potentials during the surgical treatment of tumors of the chiasmal-sellar area].	visual evoked potentials were recorded in different stages of the operation in 12 patients with tumors of the chiasmal-sellar region. a correlation was noted between the dynamics of the p100 latent period and the changes of visual functions after the operation. intraoperative monitoring of visual evoked potentials makes it possible to appraise the functional condition of the optic nerves objectively and correct the techniques and tactics of the operative intervention.	2007	2168648
reduced incidence of bone metastases in irradiated areas after external radiation therapy of prostatic carcinoma.	fourteen males, out of 380 patients, treated with radiation to the central pelvis and lumbar spine for poorly differentiated prostatic carcinoma were analyzed in retrospect. the dose of radiation to the bones of the target area was 5,000 cgy. the patients showed no signs of metastases at bone scintigraphy performed in connection with the treatment. in an average of 34 months after finishing radiotherapy, the patients developed metastases at bone scintigraphy. the pattern was similar in all patie ...	2007	2045303
endogenous n-nitrosation and cancer of the biliary tract.	evidence is presented that n-nitroso compounds occur in bile from patients who have undergone surgery for gallstones or had gastrectomy and from unoperated persons. it is unlikely, therefore, that local formation of nitrosamines can account for the excess risk for gallbladder cancer in the first two groups. gastric formation remains the likeliest hypothesis.	2007	1855836
[lobac caused a life-threatening burn-like skin injury].		2007	1865722
cognitive and affective disorders in elderly diabetics.	in non-insulin-dependent diabetes mellitus, performance of complex cognitive tasks requiring the storage and retrieval of new information is poorer than in age-matched controls. by contrast, performance of less demanding tasks such as immediate memory and simple reaction time is essentially equivalent for niddm patients and controls. this pattern parallels the cognitive change observed with normal aging, in which age differences are minimal on less demanding immediate memory tasks but older adul ...	2007	2224744
observation and theory in psychoanalysis: the self psychology of heinz kohut.	the development of self psychology by heinz kohut illustrates the influence of observational method on theory formation in psychoanalysis. in 1959 kohut began to emphasize empathy and introspection over the traditional combination of free association and evenly suspended attention. this became an important part of a process of revision in psychoanalytic theory which culminated in self psychology. this paper demonstrates how and why that particular change in observational method influenced that p ...	2007	1924603
preventing the kidney disease of diabetes mellitus: public health perspectives.		2007	2643304
seventh international meeting of the society for advancement of contraception. singapore, 4-11 november 1990.		2007	1950709
architectonics of the parietal and temporal association cortex in the strepsirhine primate galago compared to the anthropoid primate macaca.	a number of higher order association areas have been described in the parietal and temporal cortex of large-brained anthropoid primates such as macaca. however, little is known about the evolution of these areas, and the existence of homologous areas has not yet been clearly demonstrated in other mammalian groups. we addressed this issue by comparing the myelo- and cytoarchitecture of posterior association cortex in the anthropoid macaca to that of the small-brained, strepsirhine ("prosimian") p ...	2007	1939733
validation of biologic markers for use in research on chronic fatigue syndrome.	unresolved aspects of chronic fatigue syndrome can be addressed by research involving biologic markers. these may be any molecular, biochemical, physiological, or other biologic parameter obtainable from biologic specimens. the use of biologic markers in research requires their validation as dependent or independent variables. additionally, other characteristics of markers such as reliability of assays, background level, confounding factors, interpretations, and legal and ethical implications sh ...	2007	2020808
[influence of the lithiasic surface on the strategy: complications and results in the treatment of kidney calculi].	stone mass is a fundamental parameter that determines the approach to reno-ureteral lithiasis. reference to stone size based on a single diameter gives us an imprecise idea about stone volume. for this reason, we recommend using the term "stone surface" to express stone mass. stone surface is obtained by multiplying the longitudinal by the transversal diameter expressed in millimeters, using the plain film for radiopaque and the urogram for radiolucent calculi. stone surface is a parameter which ...	2007	2699547
[estimation of passive smoking during pregnancy by cotinine measurement and its effect on fetal growth].	maternal cigarette smoking has been associated with some complications of pregnancy, including low birth weight and increased morbidity. recently, it has been reported that maternal passive smoking also affects the fetal environment and causes fetal growth disturbance. in this study, we aimed to investigate the effects of maternal passive smoking on pregnant women and their fetuses by measuring cotinine concentrations in maternal urine and umbilical cord blood. the results were as follows: 1) am ...	2007	2746069
epidemiological surveillance. development of an international sentinel network of general practitioners (eurosentinel).		2007	2223567
epidemiological surveillance. development of an international sentinel network of general practitioners (eurosentinel).		2007	2223567
is honesty the best policy?		2007	2391008
on the treatment of enchondroma.	we recommend simply removing an enchondroma without filling the cavity with cancellous bone or plaster-of-paris. this method can also be applied to other benign bone conditions, such as aseptic necrosis.	2007	2230499
aids literature index.		2007	2083300
trade and generic names of drugs.		2007	2234623
[clinical evaluation of 70 pediatric renal transplants after 10 to 17 years].	among 149 kidneys transplanted in 141 children and teenagers aged 4 1/2 to 20 years, from november 1972 through december 1979, 70 (47%) were still functioning after ten years (67/145 cadaver donor grafts and 3/4 living related donor grafts). medical and social status at the last follow-up visit (10 to 16 1/2 years after transplantation; m = 12 years) was analyzed. patients were divided into five groups on the basis of glomerular filtration rate (gfr; ml/min/1.73 m2) and blood pressure (bp): 1) g ...	2007	1929108
[prediction on the efficacy of fluoroquinolones for anaerobic infections using monte carlo simulation].	various analysis based on pk-pd (pharmacokinetics-pharmacodynamics) theory were performed for prediction on the efficacy of antimicrobial agents for infectious diseases. however, there have been few reports when causative organisms would be anaerobic bacteria. although the antimicrobial activity of fluoroquinolones against anaerobes has been weak, gatifloxacin (gflx), moxifloxacin (mflx), and garenoxacin (grnx) are considered as anti-anaerobic quinolones in recent days. therefore, we investigate ...	2007	18447205
[case report: positive signals from automated blood-culture system with negative direct gram examination].	detection of positive haemoculture is usually managed by an automated system. when a bottle is detected positive but that the gram coloration does not reveal germs by direct examination, transfer onto chocolate blood agar generally allows to confirm or infirm bacteraemia. in light of a case of fusobacterium nucleatum bacteraemia, we discuss the opportunity of pairing it with an enrichment broth. m. n, hospitalized in the hepatogastroenterology department, runs a fever of undetermined origin. thr ...	2007	18031953
early soft tissue healing around one-stage dental implants: clinical and microbiologic parameters.	despite the potential significance of early soft tissue healing to long-term outcomes, this aspect of one-stage dental implants has not been investigated. the purpose of this prospective study was to characterize clinical and microbiologic parameters of early soft tissue healing around dental implants placed following a one-stage protocol.	2007	18062110
effect of rinsing with an essential oil-containing mouthrinse on subgingival periodontopathogens.	studies have shown that the nature and amount of supragingival plaque can influence the composition of the contiguous subgingival flora. the purpose of this study was to investigate the effect of rinsing with an essential oil antimicrobial mouthrinse on levels of representative subgingival bacteria in subjects with mild to moderate periodontitis.	2007	18062115
soluble cd14 discriminates slight structural differences between lipid as that lead to distinct host cell activation.	soluble cd14 (scd14) in serum is known to sensitize host cells to lps. in the present study, the contributions of scd14 and lps-binding protein to a lipid a moiety from lps preparations of periodontopathogenic fusobacterium nucleatum sp. nucleatum were compared with that of escherichia coli-type synthetic lipid a (compound 506). f. nucleatum lipid a was identified to be a hexa-acylated fatty acid composed of tetradecanoate (c(14)) and hexadecanoate (c(16)), similar to dodecanoate (c(12)) and c(1 ...	2007	18025213
effect of chlorhexidine/thymol and fluoride varnishes on dental biofilm formation in vitro.	this study evaluated the effect of chlorhexidine/thymol (chx/t) and fluoride (f) varnishes on biofilm formation in vitro. hydroxyapatite discs coated with varnish were first immersed in saline for 0, 3, 7 or 14 d, then immersed in pasteurized saliva. the discs were incubated for 20 h with a bacterial suspension containing actinomyces naeslundii, fusobacterium nucleatum, streptococcus oralis, and veillonella dispar. uncoated discs were used as controls. growth of bacteria on the discs was evaluat ...	2007	18028054
the inhibition of dentifrice containing the lotus leaf-derived inhibitor on periodontitis-related bacteria in vitro.	to search for a substance in lotus leaf that could efficiently be used in toothpaste for preventing periodontal disease.	2007	17992914
the trimeric periplasmic chaperone skp of escherichia coli forms 1:1 complexes with outer membrane proteins via hydrophobic and electrostatic interactions.	the interactions of outer membrane proteins (omps) with the periplasmic chaperone skp from escherichia coli are not well understood. we have examined the binding of skp to various omps of different origin, size, and function. these were ompa, ompg, and yaet (omp85) from escherichia coli, the translocator domain of the autotransporter nalp from neisseria meningitides, foma from fusobacterium nucleatum, and the voltage-dependent anion-selective channel, human isoform 1 (hvdac1) from mitochondria. ...	2007	17928002
antimicrobial activity of mahonia aquifolium and two of its alkaloids against oral bacteria.	extracts or alkaloids isolated from mahonia aquifolium exhibit antimicrobial activity against gram-positive and gram-negative bacteria, fungi, and protozoa. in this study the bacteriostatic and bacteriocidal activities of a m. aquifolium extract and two of its major alkaloids, berberine chloride and oxyacanthine sulphate, were tested in vitro against nine different oral bacteria. minimum inhibitory concentrations were in the range from < or = 0.0031% to 0.1993% for the m. aquifolium extract, fro ...	2007	18072463
interleukin-1alpha stimulation in monocytes by periodontal bacteria: antagonistic effects of porphyromonas gingivalis.	periodontal pathogenic bacteria are associated with elevated levels of interleukin-1alpha (il-1alpha) but it is unclear if all species can induce cytokine production equally. porphyromonas gingivalis may be able antagonize il-1alpha induced by other species through the activity of its proteases or lipopolysaccharide (lps). monomac-6 cells and primary human monocytes were treated with culture supernatants from porphyromonas gingivalis, fusobacterium nucleatum, campylobacter rectus, actinobacillus ...	2007	17241171
[the development and in vitro experiment study of a bio-type root canal filling sealer using calcium phosphate cement].	the purpose of this study is to develop a novel root canal filling sealers based on calcium phosphate cement (cpc), and to evaluate its physical-chemical properties and in vitro antibacterial activity on the predominant bacteria infecting root canal.	2007	18004487
actinobacillus actinomycetemcomitans lipopolysaccharide regulates matrix metalloproteinase, tissue inhibitors of matrix metalloproteinase, and plasminogen activator production by human gingival fibroblasts: a potential role in connective tissue destruction.	fibroblasts, a major constituent of gingival connective tissue, can produce immunoregulatory cytokines and proteolytic enzymes that may contribute to tissue destruction. in this study, we evaluated the production of matrix metalloproteinases (mmps), tissue inhibitors of mmps (timps), and plasminogen activators by gingival fibroblasts stimulated with lipopolysaccharides (lps) produced by periodontopathogens, including actinobacillus actinomycetemcomitans. in addition, changes in the expression an ...	2007	17299802
gingival epithelial cell transcriptional responses to commensal and opportunistic oral microbial species.	transcriptional profiling and ontology tools were utilized to define the biological pathways of gingival epithelial cells modulated by coculture with the oral commensal streptococcus gordonii and the opportunistic commensal fusobacterium nucleatum. overall, f. nucleatum and s. gordonii perturbed the gingival epithelial cell transcriptome much less significantly than the oral pathogens porphyromonas gingivalis and aggregatibacter actinomycetemcomitans perturbed the transcriptome, indicating that ...	2007	17307939
improved diagnosis specificity in bone and joint infections using molecular techniques.	the microbiological diagnosis of osteoarticular infections currently relies on microbiological cultures, to specifically target treatment. however, these conventional methods sometimes lack of sensitivity and of specificity to establish definitive diagnosis. this study was conducted to determine whether molecular method could improve bacterial bone and joint infection diagnosis.	2007	18029022
efficacy of the de novo-derived antimicrobial peptide wlbu2 against oral bacteria.	the efficacy of a novel synthetic antimicrobial peptide (wlbu2) was evaluated against three oral microorganisms (grown planktonically): streptococcus gordonii, fusobacterium nucleatum, and porphyromonas gingivalis. wlbu2 killed all three species, with f. nucleatum being the most susceptible. wlbu2 also reduced the bacterial burden of s. gordonii and f. nucleatum biofilms.	2007	17325219
effect of tissue fluids on hydrophobicity and adherence of enterococcus faecalis to dentin.	this in vitro study was carried out to determine (1) the hydrophobicity of selected oral bacteria, (2) the influence of growth media (saliva and serum) and mode of growth (planktonic or biofilm) on the hydrophobicity of enterococcus faecalis, and (3) the influence of growth media and conditioning fluids on the adherence of e. faecalis to dentin. the ability to bind to a hydrocarbon phase (xylene) was used as an index of relative hydrophobicity of cells. fluorescent microscopy-based technique was ...	2007	18037050
differential activation of nf-kappab and gene expression in oral epithelial cells by periodontal pathogens.	to investigate the molecular effects of the periodontopathogens fusobacterium nucleatum (fn) and porphyromonas gingivalis (pg) on the oral epithelium, the h400 oral epithelial cell line was cultured in the presence of non-viable bacteria. following confirmation of the presence of transcripts for the bacterial pattern recognition receptors in h400 cells, toll-like receptors -2, -4 and -9, and components of the nf-kappab signalling pathway, immunocytochemical analyses were performed showing that n ...	2007	17355248
case report: lemierre syndrome presenting with fever and pharyngitis.		2007	17429891
the in vivo dynamics of streptococcus spp., actinomyces naeslundii, fusobacterium nucleatum and veillonella spp. in dental plaque biofilm as analysed by five-colour multiplex fluorescence in situ hybridization.	the formation and composition of dental plaque biofilm in vivo are important factors which influence the development of gingivitis, caries and periodontitis. studying dental plaque biofilm in in vitro models can cause an oversimplification of the real conditions in the oral cavity. in this study, bovine enamel slabs were fixed in an individual acrylic appliance in situ to quantify dental plaque formation and composition using multiplex fluorescence in situ hybridization (fish) and confocal laser ...	2007	17446294
sonoporation is an efficient tool for intracellular fluorescent dextran delivery and one-step double-crossover mutant construction in fusobacterium nucleatum.	studies of microorganisms are often hindered by a lack of effective genetic tools. one such example is fusobacterium nucleatum, a gram-negative anaerobe associated with various human infections, including those causing periodontal disease and preterm birth. the first double-crossover allelic-exchange mutant in f. nucleatum was recently constructed using sonoporation, a novel ultrasound-mediated intracellular delivery method, demonstrating potential for bacterial gene transfection. to better unve ...	2007	17449701
rat model of polymicrobial infection, immunity, and alveolar bone resorption in periodontal disease.	one of the predominant polymicrobial infections of humans is expressed clinically as periodontal disease. porphyromonas gingivalis, treponema denticola, and tannerella forsythia have been strongly implicated as members of a pathogenic consortium in the etiology of adult periodontitis. in this study we hypothesized that p. gingivalis, t. denticola, and t. forsythia are synergistic in terms of virulence potential and induce chronic periodontal inflammation that leads to alveolar bone resorption in ...	2007	17210663
compressed mints and chewing gum containing magnolia bark extract are effective against bacteria responsible for oral malodor.	flavors and natural botanic extracts are often used in chewing gum and compressed mints for breath freshening and relief of oral malodor. the oral malodor is a result of bacterial putrification of proteinaceous materials from food or saliva. in this study, magnolia bark extract (mbe) and its two main components, magnolol and honokiol, were evaluated by the minimum inhibition concentration (mic) test. the inhibitory effect of mbe mint was further evaluated by a kill-time assay study. in addition, ...	2007	17949053
evaluation of the microbiota of primary endodontic infections using checkerboard dna-dna hybridization.	the aim of this study was to evaluate the composition of the microbiota of primary endodontic infections in 111 selected cases of single-rooted teeth with necrotic pulp.	2007	17949342
improved accuracy in terminal restriction fragment length polymorphism phylogenetic analysis using a novel internal size standard definition.	terminal restriction fragment length polymorphism (t-rflp) analysis is commonly used to analyze microbial communities, including oral microflora. however, accurate identification of terminal restriction fragment (t-rf) origins is prevented by unpredictable errors in sizing, thus necessitating the clone library analysis. to minimize sizing errors, we proposed optimizing the size definition of internal standards.	2007	17949346
hyperactivity and reactivity of peripheral blood neutrophils in chronic periodontitis.	some evidence exists that peripheral neutrophils from patients with chronic periodontitis generate higher levels of reactive oxygen species (ros) after fcgamma-receptor stimulation than those from healthy controls. we hypothesized that peripheral neutrophils in periodontitis also show both hyper-reactivity to plaque organisms and hyperactivity in terms of baseline, unstimulated generation and release of ros. peripheral neutrophils from chronic periodontitis patients and age/sex/smoking-matched h ...	2007	17223966
innate immune responses of gingival epithelial cells to nonperiodontopathic and periodontopathic bacteria.	we have previously reported different susceptibilities of periodontopathic and nonperiodontopathic bacteria to antimicrobial peptides and phagocytosis by neutrophils. differences between the two groups of bacteria may exist also in their ability to induce immune responses from the host. therefore, we evaluated the effects of various oral bacteria on innate immune responses by gingival epithelial cells.	2007	17956462
antianaerobe activity of ceftobiprole, a new broad-spectrum cephalosporin.	agar dilution testing of 463 anaerobes showed most gram-positive beta-lactamase-negative strains (other than some clostridium difficile and peptostreptococcus anaerobius), as well as both beta-lactamase-positive and beta-lactamase-negative strains of fusobacterium nucleatum, to have ceftobiprole mic values of < or =0.016 to 4 microg/ml. ceftobiprole was less active against beta-lactamase-positive gram-negative bacilli, especially the members of the bacteroides fragilis group. like ceftobiprole, ...	2007	17240107
antimicrobial effect of propolis and other substances against selected endodontic pathogens.	the aim of this study was to determine the antimicrobial effect of ethanol extract of propolis (eep) and intracanal medicaments calcium hydroxide, camphorated paramonochlorophenol, and formocresol by means of the macrodilution method using the reinforced clostridial medium (rcm) and brucella and brain heart infusion media.	2007	17964476
comparison of curet and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction.	the outcome of microbiological diagnostics may depend on the sampling technique. it was the aim of the present study to compare two widely used sampling techniques for subgingival bacteria using quantitative real-time polymerase chain reaction.	2007	17470026
effect of an essential oil-containing antimicrobial mouthrinse on specific plaque bacteria in vivo.	this study was conducted to investigate the effect of rinsing with an essential oil-containing mouthrinse on levels of specific supra and subgingival bacteria in subjects with gingivitis.	2007	17535287
bacterial species in subgingival plaque and oral bone loss in postmenopausal women.	oral bacteria are widely recognized in the etiology of periodontal disease. we investigated the prevalence of subgingival bacterial infection with eight species, tested associations between infection and oral bone loss, and assessed potential confounding factors and effect modifiers of those associations in a large community-based cohort of postmenopausal women.	2007	17539719
a novel tem contrasting technique for extracellular polysaccharides in in vitro biofilms.	in the past, the visualization of the extracellular matrix of biofilms on an ultrastructural level was hampered by shrinkage artifacts. in addition, the reproducible contrasting of extracellular polysaccharides (eps) has not satisfactorily been solved. here we describe a method overcoming these difficulties, which produces artifact-free transmission electron microscopic (tem) images using multispecies biofilms grown in vitro. sufficient contrast was achieved by replacing schiff's reagent with th ...	2007	17557287
[lemierre syndrome].		2007	17633598
targeted profiling of oral bacteria in human saliva and in vitro biofilms with quantitative real-time pcr.	an in vitro plaque model based on the use of human salivary bacteria and tooth-like surfaces was previously developed for studying the formation of oral biofilm and its use for pre-clinical testing of candidate antimicrobial or antiplaque agents. in this study, a quantitative taqman pcr assay (qpcr) was developed to compare the bacterial compositions of in vitro biofilms to parent saliva samples, and to determine the relative contributions of different species in the formation of the oral biofil ...	2007	17653931
effects of glass ionomer and microfilled composite subgingival restorations on periodontal tissue and subgingival biofilm: a 6-month evaluation.	this 6-month study evaluated the effects of resin-modified glass-ionomer cement (rmgi) and microfilled composite (mc) subgingival restorations on periodontal tissues and subgingival biofilm.	2007	17668971
the impact of a low-frequency chlorhexidine rinsing schedule on the subgingival microbiota (the teeth clinical trial).	information on the efficacy of chlorhexidine (chx) rinsing on the subgingival microbiota is limited. this study tested if intermittent chx rinsing over 5 years had an impact on the subgingival microbiota.	2007	17760545
comparison of growth and susceptibility to sodium hypochlorite of mono- and dual-species biofilms of fusobacterium nucleatum and peptostreptococcus (micromonas) micros.	this study compared growth and susceptibility to different concentrations of sodium hypochlorite (naocl) of mono- and dual-species biofilms of fusobacterium nucleatum or peptostreptococcus micros in vitro at 24 hours or 96 hours. a mann-whitney u test revealed that although at 24 hours dual-species biofilms had similar viable counts to those of monospecies biofilms (p > 0.001), they were more resistant to naocl (p < 0.001). at 96 hours, both microorganisms had higher viable counts and were more ...	2007	17804319
genome sequence of fusobacterium nucleatum subspecies polymorphum - a genetically tractable fusobacterium.	fusobacterium nucleatum is a prominent member of the oral microbiota and is a common cause of human infection. f. nucleatum includes five subspecies: polymorphum, nucleatum, vincentii, fusiforme, and animalis. f. nucleatum subsp. polymorphum atcc 10953 has been well characterized phenotypically and, in contrast to previously sequenced strains, is amenable to gene transfer. we sequenced and annotated the 2,429,698 bp genome of f. nucleatum subsp. polymorphum atcc 10953. plasmid pfn3 from the stra ...	2007	17668047
co-adhesion and biofilm formation by fusobacterium nucleatum in response to growth ph.	fusobacterium nucleatum is a gram-negative anaerobic organism considered to play an important role in the progression of periodontal disease and is commonly found in clinical infections of other body sites. apart from its metabolic versatility, its cell-surface properties enable it to attach to epithelial cells, collagen, gingival epithelial cells and other bacterial genera, but not with other fusobacteria. the development of periodontitis is associated with a rise in ph in the gingival sulcus t ...	2007	17540586
activation of protective responses in oral epithelial cells by fusobacterium nucleatum and human beta-defensin-2.	oral epithelia are constantly exposed to non-pathogenic (commensal) bacteria, but generally remain healthy and uninflamed. fusobacterium nucleatum, an oral commensal bacterium, strongly induces human beta-defensin-2 (hbd2), an antimicrobial and immunomodulatory peptide, in gingival epithelial cells (gecs). hbd2 is also expressed in normal oral tissue leading to the hypothesis that oral epithelia are in an activated state with respect to innate immune responses under normal in vivo conditions. in ...	2007	17577065
the effect of mineral trioxide aggregate on phagocytic activity and production of reactive oxygen, nitrogen species and arginase activity by m1 and m2 macrophages.	to assess the influence of co-culture with mineral trioxide aggregate (mta) on phagocytosis and the production of reactive oxygen intermediates (roi) and nitrogen (no) species and the arginase activity by m1 and m2 peritoneal macrophages.	2007	17627697
development of a genus-specific pcr assay for the molecular detection, confirmation and identification of fusobacterium spp.	a genus-specific polymerase chain reaction (pcr)-based assay is developed for the detection and identification of clinically relevant fusobacterium species, including f. nucleatum and f. necrophorum. two 16s ribosomal dna (rdna) primers, fuso1 (forward primer: 5'-gag aga gct ttg cgt cc-3' [17-mer]) and fuso 2 (reverse primer: 5'-tgg gcg ctg agg ttc gac -3' [18-mer]) are designed to target conserved regions of the 16s rdna gene for fusobacterium spp. subsequent proof-of-principle studies employin ...	2007	17633142
odontogenic bacteria in periodontal disease and resistance patterns to common antibiotics used as treatment and prophylaxis in odontology in spain.	resistance in streptococci or gram-negative bacteria is associated with antibiotic consumption. scarce information exists on the antibiotic susceptibility of bacterial isolates from patients with periodontitis in countries with high antibiotic consumption, as this is an area in which microbiological testing is not performed in daily practice. the present study was undertaken to explore the susceptibility of bacterial isolates in periodontitis to antibiotics prescribed in odontology in spain as t ...	2007	17530037
in vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens.	propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. in the present study, the antimicrobial activity of five propolis samples, collected from four different regions in turkey and from brazil, against nine anaerobic strains was evaluated. ethanol extracts of propolis (eep) were prepared from propolis samples and we determined minimum inhibitory concentrations (mic) and minimum bactericidal concentrations (mbc) of eep ...	2007	17475517
antimicrobial effect of triclosan and triclosan with gantrez on five common endodontic pathogens.	microbial control of the root canal system is one of the key objectives of root canal therapy. triclosan is a widely accepted broad spectrum antimicrobial agent proven to be effective against many gram-positive and gram-negative bacteria. triclosan acts by blocking bacterial fatty acid biosynthesis. the addition of gantrez copolymer has been shown to enhance the antimicrobial activity of triclosan. the purpose of this study was to determine the minimum inhibitory and bactericidal concentrations ...	2007	17889698
implant surface analysis and microbiologic evaluation of failed implants retrieved from smokers.	the aim of this study was to evaluate the microbiota and surface of failed titanium dental implants from 4 manufacturers. twelve mobile dental implants were retrieved from 10 smokers after 3 to 10 years of functional loading. before implant removal, microbial samples were taken and evaluated using polymerase chain reaction. after implant removal, analyses of the failed implant surfaces were performed using scanning electron microscopy and energy-dispersive spectrometer x-ray. periodontal pathoge ...	2007	17912965
functionalization of pvdf membranes with carbohydrate derivates for the controlled delivery of chlorhexidin.	maltodextrin (mx) was fixed onto pvdf membranes in order to create a drug delivery guided tissue regeneration (gtr) device with controlled drug delivery properties. pvdf microporous membranes were treated by a mixture of mx and citric acid, resulting to an 18 wt% increase of the supports. mx grafted membrane could capture 103 mg/g chlorhexidin digluconate (digchx) instead of 1mg/g for a virgin membrane. a neutralization step was performed before the biological tests. viability tests confirmed th ...	2007	17804290
hemoglobin and lps act in synergy to amplify the inflammatory response.	vascular disruption and bleeding during periodontitis likely increase the levels of hemoglobin in gingival crevicular fluid. the aim of this study was to investigate the effect of hemoglobin on the inflammatory responses of human macrophages stimulated with lipopolysaccharides (lps) isolated from periodontopathogens. the production of interleukin-1 beta (il-1beta), il-6, il-8, and tumor necrosis factor alpha (tnf-alpha) by macrophages following challenges with porphyromonas gingivalis and fusoba ...	2007	17720859
fusobacterium nucleatum induces fetal death in mice via stimulation of tlr4-mediated placental inflammatory response.	intrauterine infection plays a pivotal role in preterm birth (ptb) and is characterized by inflammation. currently, there is no effective therapy available to treat or prevent bacterial-induced ptb. using fusobacterium nucleatum, a gram-negative anaerobe frequently associated with ptb, as a model organism, the mechanism of intrauterine infection was investigated. previously, it was shown that f. nucleatum induced preterm and term stillbirth in mice. fusobacterial-induced placental infection was ...	2007	17675512
antimicrobial profiles of prevotella spp. and fusobacterium nucleatum isolated from periodontal infections in a selected area of southern italy.	in view of the increasing antibiotic resistance of anaerobic gram-negative bacteria, we determined the antimicrobial profile of 55 periodontal anaerobic gram-negative bacteria correlated with human infections, comprising 16 strains of fusobacterium nucleatum and 39 strains of prevotella spp. isolated from periodontal pockets of 26 adults suffering from chronic periodontitis. all the strains of f. nucleatum were susceptible to amoxicillin/clavulanic acid, doxycycline, metronidazole, moxifloxacin ...	2007	17954025
[alterations of salivary flow rate and oral main pathogens in miniature pig with bilateral parotid gland atrophy].	to observe the effect of bilateral parotid gland atrophy on the whole saliva flow rate and the growth of main oral pathogens in different sites of oral cavity.	2007	18476559
inhibitory effect of cranberry polyphenol on biofilm formation and cysteine proteases of porphyromonas gingivalis.	the purpose of this study was to investigate the effects of cranberry polyphenol fraction on biofilm formation and activities of arg-gingipain and lys-gingipain in porphyromonas gingivalis.	2007	17956474
phylogenetic analysis of bacterial and archaeal species in symptomatic and asymptomatic endodontic infections.	phylogenetic analysis of bacterial and archaeal 16s rrna was used to examine polymicrobial communities within infected root canals of 20 symptomatic and 14 asymptomatic patients. nucleotide sequences from approximately 750 clones amplified from each patient group with universal bacterial primers were matched to the ribosomal database project ii database. phylotypes from 37 genera representing actinobacteria, bacteroidetes, firmicutes, fusobacteria and proteobacteria were identified. results were ...	2007	17172525
the proteomic profile of fusobacterium nucleatum is regulated by growth ph.	fusobacterium nucleatum is a saccharolytic gram-negative anaerobic organism believed to play an important role in the microbial succession associated with the development of periodontal disease. its genome contains niche-specific genes shared with the other inhabitants of dental plaque, which may help to explain its ability to survive and grow in the changing environmental conditions experienced in the gingival sulcus during the transition from health to disease. the ph of the gingival sulcus in ...	2007	17185543
clinical and microbiological profiles of human immunodeficiency virus (hiv)-seropositive brazilians undergoing highly active antiretroviral therapy and hiv-seronegative brazilians with chronic periodontitis.	this study compares the periodontal clinical profile and the composition of the subgingival microbiota of human immunodeficiency virus (hiv)-seropositive and hiv-seronegative subjects with chronic periodontitis.	2007	17199544
inflammation associated with implants with different surface types.	the aim of this study was to determine the nature of the inflammatory infiltrate associated with different transmucosal implant surfaces in dogs.	2007	17224032
sensitivity of oral bacteria to 254 nm ultraviolet light.	to explore the sensitivity of bacteria commonly found in root canals to 254 nm ultraviolet (uv) light, either as individual cells or as participants of a bacterial multilayer.	2007	17229117
efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro.	ozone gas and photodynamic therapy (pdt) have been claimed to be antimicrobially effective. this study assessed their antimicrobial potential in vitro. mature six-species oral biofilms were treated as follows (n = 9 per group): (i) a 60-s application of gasiform vacuum-ozone or vacuum alone (on wet or air-dried biofilm samples); (ii) pdt (i.e. methylene blue in combination with or without a diode soft laser, and a soft laser alone); or (iii) antimicrobial solutions: immersion of biofilms for 60 ...	2007	17305720
microbiological outcomes of quadrant versus full-mouth root planing as monitored by real-time pcr.	to study the short-term microbiological changes following full-mouth compared with quadrant wise scaling and root planing (fmrp and qrp) as well as long-term effects.	2007	17309590
a comparison of implant-supported, bar- or ball-retained mandibular overdentures: a retrospective clinical, microbiologic, and immunologic study of 10 edentulous patients attending a recall visit.	clinical, microbiologic, and immunologic comparisons of the peri-implant health in edentulous volunteers wearing long-standing implant-supported ball- or dolder bar-retained mandibular overdentures were performed.	2007	17319360
response to alkaline stress by root canal bacteria in biofilms.	to determine whether bacteria isolated from infected root canals survive alkaline shifts better in biofilms than in planktonic cultures.	2007	17326786
the detection of eight putative periodontal pathogens in adult and rapidly progressive periodontitis patients: an institutional study.	periodontal disease is a commonly prevalent problem faced alike by both the developed and third world countries but showing wide variations in prevalence and severity across different geographical areas. the purpose was to identify actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), ekinella corrodens (ec), campylobacter rectus (cr), bacteroides forsythus (bf), treponema denticola (td) and fusobacterium nucleatum (fn) in indian adult periodontiti ...	2007	17347537
rapid identification of fusobacterium nucleatum and fusobacterium necrophorum by fluorescence in situ hybridization.	identification of clinically relevant fusobacterium spp. is hampered by their slow growth, their frequent occurrence in polymicrobial culture, and the low reliability of biochemical differentiation methods. a newly developed fluorescence in situ hybridization (fish) assay allowed reliable and rapid identification of fusobacterium necrophorum and fusobacterium nucleatum from culture. preliminary results show that the method offers the perspective for direct detection of these pathogens in blood c ...	2007	17350209
signaling cascades triggered by bacterial metabolic end products during reactivation of kaposi's sarcoma-associated herpesvirus.	the present studies explore the role of polymicrobial infection in the reactivation of kaposi's sarcoma-associated herpesvirus (kshv) and analyze signaling pathways activated upon this induction. we hypothesized that activation of the cellular stress-activated mitogen-activated protein kinase (mapk) p38 pathway would play a key role in the bacterium-mediated disruption of viral latency similar to that of previously reported results obtained with other inducers of gammaherpesvirus lytic replicati ...	2007	17376930
evidence of periopathogenic microorganisms in placentas of women with preeclampsia.	periodontal disease is a chronic inflammatory infectious disease that may act as a focus of infection. preeclampsia is a pregnancy-specific hypertensive disorder that often leads to maternal morbidity and mortality. acute atherosis, the placental lesion of preeclampsia, shares many histopathological features with atherosclerosis. recently, chronic infection was linked to the initiation of atherosclerosis. oral pathogens have been detected in atherosclerotic plaques, where they may play a role in ...	2007	17397314
activation of toll-like receptors 2 and 4 by gram-negative periodontal bacteria.	periodontitis is a chronic infectious disease associated with a gram-negative subgingival microflora. bacterial components stimulate, among other receptors, toll-like receptor (tlr) 2 and/or tlr4. accumulating evidence indicates that both qualitatively and quantitatively distinct immune responses result from the triggering of tlr2 as compared to tlr4 triggering. the aim was to study the interaction of porphyromonas gingivalis, actinobacillus actinomycetemcomitans, tannerella forsythensis, prevot ...	2007	17488439
infection at titanium implants with or without a clinical diagnosis of inflammation.	to assess the microbiota at implants diagnosed with peri-implantitis, implant mucositis, or being clinically healthy.	2007	17517058
periodontal bacterial dna suppresses the immune response to mutans streptococcal glucosyltransferase.	certain cpg motifs found in bacterial dna enhance immune responses through toll-like receptor 9 (tlr-9) and may also demonstrate adjuvant properties. our objective was to determine if dna from bacteria associated with periodontal disease could affect the immune response to other bacterial antigens in the oral cavity. streptococcus sobrinus glucosyltransferase (gtf), an enzyme involved in dental caries pathogenesis, was used as a test antigen. rowett rats were injected with aluminum hydroxide (al ...	2007	17517867
physiological alterations of a fusobacterium nucleatum strain exposed to oxidative stress.	the purpose of this study was to investigate the effect of oxidative stress on physiological and genetic characteristics of fusobacterium nucleatum and its interference on this microbial identification methods.	2007	17584449
production of antagonistic substance by eikenella corrodens isolated from the oral cavity of human beings with and without periodontal disease.	antagonistic abilities may confer ecological advantages for micro-organisms in competitive ecosystems. however, reports regarding this phenomenon in eikenella corrodens are not available.	2007	17584471
fada from fusobacterium nucleatum utilizes both secreted and nonsecreted forms for functional oligomerization for attachment and invasion of host cells.	fusobacterium nucleatum is a gram-negative anaerobe associated with various human infections, including periodontal diseases and preterm birth. a novel fada adhesin was recently identified for host-cell binding. it consists of 129 amino acid residues, with an 18-amino acid signal peptide. expression of fada in escherichia coli enhanced bacterial binding to host epithelial and endothelial cells. in both e. coli and f. nucleatum, fada exists in two forms, the intact pre-fada and the secreted matur ...	2007	17588948
association of a high-molecular weight arginine-binding protein of fusobacterium nucleatum atcc 10953 with adhesion to secretory immunoglobulin a and coaggregation with streptococcus cristatus.	fusobacterium nucleatum coaggregates with a diverse range of bacterial species, and binds to host tissues and proteins such as immunoglobulin. these interactions may support the attachment of a variety of organisms to oral surfaces and can facilitate the invasion of soft tissues. we hypothesized that coaggregation with streptococci and immunoglobulin binding may occur by a common adhesin sensitive to l-arginine.	2007	17600532
cultivable bacteria in infected root canals as identified by 16s rrna gene sequencing.	introduction: traditionally, cultivable bacteria isolated from infected root canals have been identified by phenotype-based methods. because 16s ribosomal rna (rrna) gene sequencing has emerged as a more accurate and reliable tool for bacterial identification, the present study applied this approach to identify bacterial isolates recovered from the root canals of teeth with chronic apical periodontitis. methods: anaerobic techniques were used for culturing; identification of the isolates was car ...	2007	17600539