Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [effects of ethidium bromide on the viscosity of chromatin secreted by rat liver in normal state and after denervation]. | the paper studies structural state of chromatin secreted from the rat's liver. for this purpose the authors studied the dependence of relative viscosity of chromatin preparations secreted from the liver of rats and three groups of animals being in different terms of postdenervation process. | 1990 | 2083357 |
| cell surface proteins from shigella dysenteriae type 1. | a simple extraction procedure was used for preparing cell surface proteins (csps) from shigella dysenteriae type 1. the preparations obtained using either buffer or water extractions were free from lipopolysaccharide (lps), as well as cytoplasmic and periplasmic proteins. by sds-page, about 25 polypeptides were detected, and western-blot analysis recognised 15 polypeptide antigens. when analysed by crossed immunoelectrophoresis, using anti-shigella dysenteriae type 1 rabbit sera, 18 antigenic ba ... | 1990 | 2206198 |
| kinetics of "p"-site-mediated inhibition of adenylyl cyclase and the requirements for substrate. | the kinetics of "p"-site-mediated inhibition of adenylyl cyclase was studied with the detergent-solubilized enzyme from rat brain. mn2(+)-activated adenylyl cyclase exhibited typical noncompetitive inhibition by 2'-d3'-amp or 2',5'-dideoxyadenosine (2',5'-ddado). however, enzyme that was preactivated with guanosine 5'-o-(3-thiotriphosphate) (gtp gamma s) or proteolytically with ninhibin (+ gtp gamma s) exhibited apparently uncompetitive inhibition with either 2'-d3'-amp or 2',5'-ddado and with e ... | 1990 | 2365688 |
| [bacillary dysentery--1988]. | 1990 | 2251385 | |
| [bacteriology and epidemiology of shigellas isolated at the army hospital in djibouti]. | in a space of 26 months 108 shigellas were isolated from 104 patients with sporadic and benign diseases. 90% of the subjects were young french. both s. dysenteriae and s. boydii make up 21% of the strains studied. 13% of the germs are multiresistance. multiresistance can enhance development of serious sickness if a virulent strain comes in. | 1990 | 2263188 |
| fatty streak development in the vicinity of aortic coarctations in hypercholesterolemic rabbits. | the influence of altered local hemodynamics on fatty streak development in rabbits fed high cholesterol diets was investigated. an aortic coarctation was created in the abdominal aorta of nine rabbits by placing a partially constricting gold or silver band (1.7 mm x 10 mm) around the aorta between the renal arteries and aortic bifurcation. controls were 20 rabbits; seven sham operated and 13 unoperated rabbits. the abdominal aorta 1-2 cm proximal to the coarctation showed lipid deposition involv ... | 1990 | 2271758 |
| bovine spongiform encephalopathy (bse): menace or mirage? | 1990 | 2271904 | |
| [role of protons and bicarbonate in adaptative gastric cytoprotection]. | the unknown mechanism of adaptative gastric cytoprotection (agc) induced by 20% ethanol and subsequent injury with 70% ethanol was studied in wistar rats. pretreatment with indomethacin or hgii2 did not prevent the agc, there suggesting that neither endogenous pgs nor gastric mucus take part in its mechanism. on the other hand, ranitidine pretreatment blocked and even aggravated the damage induced by ethanol-ethanol. in contrast, the latter phenomenon was reverted by 20% acidified ethanol. it is ... | 1990 | 2275313 |
| immunolabelling of shiga toxin in macrophages infected with shigella dysenteriae 1. | immunolabelling of shiga toxin in macrophages infected with a non-invasive shigella dysenteriae 1 isolate showed that bacteria remained alive for 3 h after ingestion within the phagocytic vacuole and synthesized shiga toxin. the normal process of toxin secretion was, however, impaired by the phagosomal environment and toxin molecules accumulated within the bacterial cytoplasm. | 1990 | 2218059 |
| hemolysin as a marker for serratia. | all serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. dna fragments of the two hemolysin genes hybridized with the chromosomal dna of s. marcescens, s. liquefaciens, s. kiliensis, s. grimesii, s. proteamaculans, s. plymutica, s. rubridaea which were also hemolytic. the restriction pattern of the hemolysin locus differed in each strain. s. ficaria and s. marinorubra expressed a different hemolysin ... | 1990 | 2222120 |
| differentiation of shiga toxin and vero cytotoxin type 1 genes by polymerase chain reaction. | two sets of synthetic oligonucleotide primers were used in a polymerase chain reaction technique to distinguish genes for shiga toxin in shigella dysenteriae 1 and type 1 vero cytotoxin (vt1) in escherichia coli. vt1a and vt1b primers directed at a common 130-base-pair (bp) fragment of the stx and slti genes detected template nucleic acid in both shiga toxin-positive s. dysenteriae 1 and vt1-producing e. coli strains. vt1c and vt1d primers, targeting a 140-bp fragment of the promoter region of t ... | 1990 | 2230244 |
| plasmid profiles of shigella dysenteriae type 1 isolates from ethiopia with special reference to r-plasmids. | plasmid profiles of 80 shigella dysenteriae type 1 (shiga's bacillus) strains, collected between 1974 and 1985 in ethiopia, were examined. strains with the dominant antibiotic-resistance (r-) type--resistance to ampicillin (a), chloramphenicol (c), streptomycin (s), sulphadiazine (su) and tetracycline (t)--showed two distinct plasmid profiles (pp). six plasmid bands were demonstrated in "ethiopian strains" with pp-1a isolated between 1974 and 1982. in mating experiments with these strains, esche ... | 1990 | 2231675 |
| in vitro diffusion of lidocaine across endotracheal tube cuffs. | smooth emergence from general endotracheal anesthesia is frequently complicated by coughing induced by stimulation from an endotracheal tube. lidocaine and other local anesthetics have been shown to anesthetize important rapidly adpating stretch receptors in the dog trachea. with the aim of providing a reservoir for continuous lidocaine release to adjacent tracheal tissue, we examined the ability of clinically used concentrations of lidocaine to diffuse across a commonly used endotracheal tube c ... | 1990 | 2275911 |
| [new aspects of intolerance of the aqueous iodinated x-ray contrast medium, adipiodone--fatal interaction of adipiodone-ultrabil with the immunomodulators corynebacterium parvum (cp) and shigella dysenteriae endotoxin (lps)]. | the authors assume a new type of interaction of the aqueous x-ray contrast substance type adipiodone (ultrabil 50% spofa) with immunomodulators of corynebacterium parvum (cp) and the endotoxin of shigella dysenteriae (lps). the fatal type of interaction developed regularly as a result of the concurrent i.v. administration of the x-ray contrast substance adipiodone with the endotoxin of shigella dys. (lps) to laboratory animals (mice) which had five days previously a single dose of corynebacteriu ... | 1990 | 2237238 |
| in vitro diffusion of lidocaine across endotracheal tube cuffs. | smooth emergence from general endotracheal anesthesia is frequently complicated by coughing induced by stimulation from an endotracheal tube. lidocaine and other local anesthetics have been shown to anesthetize important rapidly adpating stretch receptors in the dog trachea. with the aim of providing a reservoir for continuous lidocaine release to adjacent tracheal tissue, we examined the ability of clinically used concentrations of lidocaine to diffuse across a commonly used endotracheal tube c ... | 1990 | 2275911 |
| mechanism of the vasodilator action of pinacidil. | the mechanism of the vasodilator action of pinacidil has been studied in rat mesenteric small arteries. the results show, first, that the use of flux studies to make measurements of ion permeability requires knowledge of the membrane potential, especially as regards k+ permeability. second, the results confirm that the vasodilator effect of pinacidil is due to an increase in k+ permeability. lastly, the results suggest that the k+ channels involved are sensitive to glibenclamide. | 1990 | 2242449 |
| enzyme-linked immunosorbent assay for shiga toxin and shiga-like toxin ii using p1 glycoprotein from hydatid cysts. | shiga toxin from shigella dysenteriae type 1 strains and shiga-like toxins (slt) i and ii from escherichia coli bind to terminal alpha-d-galp-(1----4)-d-galp containing glycolipids. hydatid cyst fluid isolated from sheep infected with echinococcus granulosus contains a glycoprotein (p1gp) with a terminal alpha-d-galp-(1----4)-d-galp disaccharide. preparations of p1gp were shown to interact directly with shiga toxin and to inhibit the binding and cytotoxicity of shiga toxin to hela cells. a sandw ... | 1990 | 2295845 |
| death in shigellosis: incidence and risk factors in hospitalized patients. | the total number of admissions and deaths of patients with shigellosis were ascertained at the dhaka treatment centre of the international centre for diarrhoeal disease research, bangladesh, 1974-1988, and the characteristics of 67 patients who died were compared with those of 134 discharged alive. of 9780 shigella-infected inpatients, 889 (9.1%) died; 32.3% of deaths occurred in children less than 1 year of age. fatality rates were highest (10.3%) in shigella sonnei-infected patients and lowest ... | 1990 | 2313128 |
| role of bacterial and host factors in the pathogenesis of shigella septicemia. | to determine the role of bacterial and host factors in the pathogenesis of shigellemia, blood and fecal shigella isolates were compared for serum resistance and siderophore production, and shigellemic patients were examined for decreased serum bactericidal activity or increased serum transferrin saturation compared to control patients with non-bacteremic shigellosis. the majority of both blood (36/38) and fecal (36/48) shigella isolates were sensitive to normal serum (greater than 2 log kill/60 ... | 1990 | 2373088 |
| a method for detecting shiga toxin and shiga-like toxin-i in pure and mixed culture. | shiga toxin and shiga-like toxins (slts, syn. verotoxins) are currently detected by tissue culture assays that are expensive, time-consuming and require specialised facilities and experienced personnel. we have developed a rapid method to detect shiga toxin and slt-i (verotoxin 1) based on their binding to globotriosyl ceramide (gb3). bound toxin was then detected by an enzyme-linked immunosorbent assay (elisa) with monoclonal antibodies. the direct detection of cytotoxins from pure culture plat ... | 1990 | 2391709 |
| functional analysis of the shiga toxin and shiga-like toxin type ii variant binding subunits by using site-directed mutagenesis. | the b subunit of shiga toxin and the shiga-like toxins (slts) mediates receptor binding, cytotoxic specificity, and extracellular localization of the holotoxin. while the functional receptor for shiga toxin, slt type i (slt-i), and slt-ii is the glycolipid designated gb3, slt-ii variant (slt-iiv) may use a different glycolipid receptor. to identify the domains responsible for receptor binding, localization in escherichia coli, and recognition by neutralizing monoclonal antibodies, oligonucleotid ... | 1990 | 2404947 |
| the colon in shigellosis: serial colonoscopic appearances in shigella dysenteriae i. | twenty-five patients (16 males and 9 females; mean age 24.7 +/- 8.4 years) with acute colitis caused by shigella dysenteriae i were studied, a total of 115 colonoscopic examinations being performed. twenty-two patients had weekly colonoscopic examinations until the last procedure revealed normal colonic mucosa. six abnormal colonoscopic appearances were documented, namely mucosal edema, ulcers, friability, punctate spots, erythematous areas and luminal exudate. mucosal edema was the dominant fin ... | 1990 | 2407526 |
| two unrelated cases of single maxillary central incisor with 7q terminal deletion. | two unrelated cases of single maxillary central incisor (sm-ci) with 7q terminal deletion of the same breakpoint at 7q36.1 were described. they had mental retardation, microcephaly, hypotelorism, short stature, and normal levels of plasma growth hormone. one case had bilateral caudal ectopic kidneys, double renal pelves, and dilated ureters. the other had bilateral hydroureteronephrosis. the present cases suggest that 7q terminal deletion is one of the causes of smci. | 1990 | 2094780 |
| [aminoacyl-trna synthetases (codases) and their noncanonical functions]. | the aim of this review is to summarize the data obtained in the author's laboratory during the last decade. the main objects of these investigations were mammalian aminoacyl-trna synthetases, mainly bovine tryptophanyl-trna synthetase (ec 6.1.1.2). the data are discussed and compared with those described in literature. in the course of these studies it turned out that some properties of mammalian aminoacyl-trna synthetases for instance, nuclear location of some of the synthetases, presence of ex ... | 1990 | 2094804 |
| in vitro activity of ljc10,627, a new carbapenem antibiotic with high stability to dehydropeptidase i. | the in vitro activity of ljc10,627, a new carbapenem, was compared with those of imipenem and ceftazidime. ljc10,627 had broad-spectrum activity against gram-positive and gram-negative clinical isolates. the mics of this compound for 90% of members of the family enterobacteriaceae tested (mic90s), including strains resistant to ceftazidime, ranged from 0.1 to 25 micrograms/ml. ljc10,627 inhibited pseudomonas aeruginosa at an mic90 of 3.13 micrograms/ml; it thus was twofold more active than imipe ... | 1990 | 2203313 |
| outbreak of dysentery due to nalidixic acid resistant s. dysenteriae 1 at agartala, tripura: a hospital based study. | during the epidemic of bacillary dysentery at agartala, tripura, a total of 62 hospitalized patients suffering from diarrhoeal diseases were studied during the later part (11-16 june, 1988) of the epidemic. principal features of shigellosis cases were discussed. of these 62 cases investigated, 19(30.6%) cases had the mucoid diarrhoea. from them s.dysenteriae type 1 and s.flexneri had been recovered from 31.6% and 10.5% cases, respectively. all the strains of s.dysenteriae type 1 isolated during ... | 1990 | 2101382 |
| multidrug resistant epidemic shigellosis in a village in west bengal, 1984. | an out break of acute bacillary dysentery in a village called dhamasin in hooghly district of west bengal was investigated during march 1984. forty seven percent of families were affected. a total of 91 cases and 2 deaths occurred amongst 937 people giving an over all attack rate of 9.7% and a case fatality rate of 2.2 percent. highest attack rate (22.7%) was observed in below one year age group. multiple drug resistant shigella dysentery type 1 strains were isolated for the first time from 6 ou ... | 1990 | 2101383 |
| construction and evaluation of live attenuated vaccine strains of shigella flexneri and shigella dysenteriae 1. | shigellosis is an invasive disease of the human colon which is particularly prevalent among children of the developing world. no proper vaccine is available to protect against this enteric disease. it is currently accepted that only live strains with attenuated virulence administered orally may elicit protective immunity at the level of the colonic mucosa, which is the exclusive site of multiplication of causative microorganisms such as shigella flexneri and shigella dysenteriae 1. we have const ... | 1990 | 2101481 |
| improved sectioning and ultrastructure of bacteria and animal cells embedded in lowicryl. | lowicryl k4m-embedded gram-positive and gram-negative bacteria have a tendency to separate between the cell surface and the resin. this often leads to distortion of bacteria and more especially of mycobacteria. we describe attempts made to overcome this technical problem. different assays were made on bacillus subtilis, escherichia coli, and mycobacterium avium: 1) modification of the bacterial surface by coating of bacteria with proteinic compounds; 2) treatment of bacteria with metallic salts ... | 1990 | 2110246 |
| [experience in using new drug preparations for treating adult patients with acute intestinal infections]. | ciprofloxacin (bayer, frg), a derivative of hydroxyquinolone acid, was used for the treatment of patients with shigellosis and salmonellosis and for the sanitation of salmonella carriers. the drug turned out to exert a positive effect on bacteriological sanitation of the body. in order to treat patients with food toxinfection of unknown etiology, use was made of intetrix (farmacos, socialist federal republic of yugoslavia). inclusion of the drug into combined treatment of patients with food toxi ... | 1990 | 2110389 |
| [the characteristics of the biological properties of shigella dysenteriae 1 circulating in the ussr and india: its biochemical activity and agglutinability]. | the properties of 71 s. dysenteriae 1 strains isolated from patients in the ussr and india in 1986-1988 were studied. the cultures possessed typical biochemical and serological properties. as revealed in this investigation, high fastidiousness of this infective agent to the quality of synthetic nutrient could become the cause of false negative reactions in different substrates used for the identification of enterobacteria, thus leading to diagnostic mistakes. the variability of the biochemical a ... | 1990 | 2097846 |
| transposition in shigella dysenteriae: isolation and analysis of is911, a new member of the is3 group of insertion sequences. | twenty-nine clear-plaque mutants of bacteriophage lambda were isolated from a shigella dysenteriae lysogen. three were associated with insertions in the ci gene: two were due to insertion of is600, and the third resulted from insertion of a new element, is911. is911 is 1,250 base pairs (bp) long, carries 27-bp imperfect terminal inverted repeats, and generates 3-bp duplications of the target dna on insertion. it was found in various copy numbers in all four species of shigella tested and in esch ... | 1990 | 2163395 |
| associations of escherichia coli k-12 ompf trimers with rough and smooth lipopolysaccharides. | the associations of both rough and smooth lipopolysaccharides (lps) with the ompf porin of escherichia coli k-12 were examined in gale strains deleted for ompc. transformation with pss37 and growth with galactose conferred the ability to assemble a shigella dysenteriae o antigen onto the core oligosaccharide of e. coli k-12 lps. the association of lps with ompf trimers was assessed by staining, autoradiography of lps specifically labeled with [1-14c]galactose, and western immunoblotting with a m ... | 1990 | 2168378 |
| [shiga and shiga-like toxins]. | 1990 | 2173117 | |
| trimethoprim resistance gene in shigella dysenteriae 1 isolates obtained from widely scattered locations of asia. | trimethoprim-resistance genes of shigella dysenteriae 1 strains, isolated from a different location of six different countries of asia over a 5-year period were characterized by using three different dihydrofolate reductase (dhfr) gene probes. the trimethoprim-resistant (tmpr) strains hybridized only with the type i dhfr gene probe by colony hybridization. none of the strains hybridized with types ii and iii dhfr gene probes. southern blot experiments using plasmid dna extracted from these resis ... | 1990 | 2182327 |
| [antibiotic resistance of strains of shigella dysenteriae and flexneri isolated in tananarive and on the east coast of madagascar]. | from november 1988 to march 1989, stools from 804 malagasy children were examined, thirty-seven strains of shigella were isolated, from which 5 s. dysenteriae serotype 1 (= shiga bacillus) strains were found to be resistant to ampicillin, carbenicillin, streptomycin, chloramphenicol, tetracycline, sulphonamide and even to trimethoprim. resistance to trimethoprim has appeared only recently in madagascar. | 1990 | 2190702 |
| comparative study of attachment to and invasion of epithelial cell lines by shigella dysenteriae. | henle 407 and hela cells were compared as hosts for shigella dysenteriae at a low multiplicity of infection. efficiency of attachment and invasion without centrifugation, as well as selectivity for pathogenic over nonpathogenic s. dysenteriae without congo red, were much greater for henle 407 cells than for hela cells. | 1990 | 2194970 |
| epidemiology of shigellosis in teknaf, a coastal area of bangladesh: a 10-year survey. | the epidemiological data on shigellosis in teknaf, a coastal area of bangladesh, were reviewed for a 10-year period (1975-84). certain similarities and differences were observed in the epidemiology of the disease in teknaf when compared with urban dhaka and rural matlab. similarities included: round-the-year infection with two peaks, one in the monsoon period and the other in the winter period; high male to female attendance ratio at the treatment centre; the predominance of infection in the und ... | 1990 | 2200700 |
| kinetic analysis of 3-quinuclidinyl 4-[125i]iodobenzilate transport and specific binding to muscarinic acetylcholine receptor in rat brain in vivo: implications for human studies. | radioiodinated r- and s-quinuclidinyl derivatives of rs-benzilate (r- and s-125iqnb) have been synthesized for quantitative evaluation of muscarinic acetylcholine receptor binding in vivo. two sets of experiments were performed in rats. the first involved determining the metabolite-corrected blood concentration and tissue distribution of tracer r-iqnb (active enantiomer) and s-iqnb (inactive enantiomer) in brain 1 min to 26 h after intravenous injection. the second involved the measurement of br ... | 1990 | 2134838 |
| plasmid profile analysis and antimicrobial susceptibility patterns of shigella isolates from nigeria. | in an epidemiological survey, plasmid profiles and antimicrobial susceptibility testing of 100 shigella isolates in lagos, nigeria was done. all the isolates were sensitive to nalidixic acid, nitrofurantoin and ciprofloxacin. the commonest antimicrobial susceptibility pattern was resistance to ampicillin, colistin sulphate, co-trimoxazole, streptomycin and tetracycline. all but 4 of 100 isolates screened contained one or more plasmids. plasmid profile analysis distinguished more strains than did ... | 1990 | 2200702 |
| plasmid profiles of antibiotic-resistant shigella dysenteriae types 2, 3, 4, 6 and 7 isolated in ethiopia during 1976-85. | plasmid profile analysis by agarose gel electrophoresis was carried out on 37 drug-resistant strains of shigella dysenteriae types 2, 3, 4, 6 and 7. these strains were collected between 1976 and 1985 in addis ababa, ethiopia. the plasmid profile of s. dysenteriae type 2 strains with r-type cssut did not show middle-sized plasmids likely to code for cssut resistance. all strains contained a large plasmid of about 120 megadaltons (mda), and a cryptic plasmid of about 2.2 mda. the plasmid profiles ... | 1990 | 2200703 |
| survey of enteropathogenic agents in children with and without diarrhoea in ghana. | a survey was carried out over 1 year in a rural area of ghana on the isolation, detection and/or identification of enteric pathogens from children under 5 years of age with and without diarrhoea. the isolation and detection rate of shigella flexneri, shigella dysenteriae, giardia lamblia and rotavirus were higher in children with diarrhoea than in controls. yersinia enterocolitica, vibrio cholerae and vibrio parahaemolyticus were not isolated during the period of this survey. the incidence of ot ... | 1990 | 2270006 |
| effects of chronic non-ketotic diabetes and aging on myocardial function and fatty acid oxidation. | the effects of aging and chronic non-ketotic diabetes on contractile properties, oxygen consumption, palmitate oxidation and morphology were studied in isolated, perfused working hearts of 2, 9, 12 and 22 month old rats. the heart rate, coronary flow, and oxygen consumption were no different among the 9, 12 and 22 month control and diabetic hearts. cardiac work was not depressed in control hearts until 22 months of age. depression of cardiac output due to aging in the control hearts progressed i ... | 1990 | 2141842 |
| [antibiotic resistance of shigella isolated in the kara-kalpak a.s.s.r. 1987-1988]. | sensitivity of shigella spp. isolated in one of the hospitals of nukus within 1987-1988 and earlier in 1977 and 1985 was studied. s. flexneri 1-5 remained the main causative agents of dysentery on the territory. however, beginning from 1987 there were registered cases of dysentery caused by s. dysenteriae 1. the isolates were most sensitive to cefotaxime, cephaloridine, polymyxin b and gentamicin. the majority of the isolates were resistant to tetracycline, levomycetin (chloramphenicol) and stre ... | 1990 | 2144726 |
| rapid and specific detection of verotoxin genes in escherichia coli by the polymerase chain reaction. | a set of four synthetic oligonucleotide probes derived from sequences of the vt1 (shiga-like toxin i [slt-i]) and vt2 (slt-ii) genes were used in a polymerase chain reaction (pcr) amplification procedure to detect these genes in some enteric pathogens. a total of 40 verotoxin-producing escherichia coli strains and 43 isolates of other recognized enteric pathogens were studied. pcr amplification products identifying the vt1 and vt2 gene sequences were observed only in nucleic acid extracted from ... | 1990 | 2182671 |
| dna sequence analysis of pgla and mechanism of export of its polygalacturonase product from pseudomonas solanacearum. | the pgla gene encodes a 52-kilodalton extracellular polygalacturonase (pga) which is associated with the phytopathogenic virulence of pseudomonas solanacearum. the nucleotide sequence of pgla and the putative amino acid sequence of the pga protein were determined. a computer search identified a 150-residue region of pga which was similar (41%) to the amino acid sequence of a region of the pg-2a polygalacturonase from tomato. comparison of the amino terminus of the pgla open reading frame with th ... | 1990 | 2193922 |
| production of shiga-like toxin among escherichia coli strains and other bacteria isolated from diarrhea in são paulo, brazil. | an elevated level of shiga-like toxin i (slt-i) production was found in 1 of 466 escherichia coli strains studied. among the 34 sonic lysates obtained from classical enteropathogenic e. coli, 5 produced slt-i. the aeromonas, citrobacter, edwardsiella, enterobacter, klebsiella, proteus, providencia, pseudomonas, salmonella, serratia, shigella, yersinia, and vibrio strains also studied were not slt producers, except for a shigella dysenteriae type 1 strain. although slt-i-producing e. coli strains ... | 1990 | 2199511 |
| polymerase chain reaction for detection of invasive shigella flexneri in food. | the polymerase chain reaction (pcr) was used to amplify a 760-base-pair (bp) fragment with the 220-kbp invasive plasmids of enteroinvasive escherichia coli, shigella flexneri, shigella dysenteriae, shigella boydii, and shigella sonnei as templates. this pcr product was easily detected by agarose gel electrophoresis. a 210-bp acci-psti fragment lying within the amplified region was used as a probe in southern hybridization blots and showed that the pcr-generated product was derived from the invas ... | 1990 | 2200336 |
| haemolytic uraemic syndrome with shigella. | a 49-year-old male developed bloody diarrhoea whilst on a visit to india. sigmoidoscopy and rectal biopsy showed acute colitis. shigella dysentery type i was isolated from stool culture. cytotoxin production by the organism was demonstrated. the patient developed acute renal failure, thrombocytopaenia and microangiopathic haemolytic anaemia. he required mechanical ventilation, haemodialysis, blood transfusion and antibiotic therapy and achieved a complete recovery. this is an unusual case of hae ... | 1990 | 2202698 |
| immunodetection of lipopolysaccharide in macrophages during the processing of non invasive shigella dysenteriae. | the location of lipopolysaccharide (lps) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive shigella dysenteriae 1 strain. bacterial degradation began only 3 h after the end of infection. the first visible sign of degradation was detected by immunogold labelling at the level of lps which detached from the bacterial surface and was transferred to the perinuclear lysosomes. after a few hours, it was found in small vesicles spread over the wh ... | 1990 | 2129020 |
| [hepato-renal polycystic disease and liver hydatidosis (a rare association)]. | a case is presented of a patient with polycystic kidneys and familial history of this disease who was from a zone of hydatid endemism. the patient had a hepatic mass compatible with hydatid cyst. diagnoses by imaging, like echography and cat, confirmed it, aside from evidencing polycystic liver disease. there was operative and anatomopathologic confirmation of both diseases of the liver. after a review of the bibliography that shows a scant incidence of this concomitance, some remarks are offere ... | 1990 | 2346686 |
| correlation between beta 2-microglobulin and soluble interleukin-2 receptor levels in plasma of individuals living in a malarial endemic region. | beta 2-microglobulin (beta 2m) levels were related to the expected immunoprotection in 81 individuals living in a malarial mesoendemic area near bobo-dioulasso (burkina faso), who were longitudinally followed. soluble interleukin-2 receptor (sil-2r) levels were positively correlated to those of beta 2m (r = 0.44; n = 237; p less than 0.001). this suggests that most of the beta 2m could have originated from activated t and b cell membrane turnover. in our study, both beta 2m and sil-2r were inver ... | 1990 | 2242964 |
| carboxy-terminal peptides from the b subunit of shiga toxin induce a local and parenteral protective effect. | two synthetic peptides corresponding to overlapping sequences from the c-terminus of the b chain of shiga toxin were prepared and characterized. these peptides consisted of residues 54-67 and 57-67 in the protein sequence. this region coincides with the major peak of surface area residues, as predicted from a computer-derived plot. for the purpose of immunization, the peptides were either conjugated with a protein or a synthetic carrier, or were polymerized. polyclonal antibodies against these p ... | 1990 | 2203962 |
| plants used in guatemala for the treatment of gastrointestinal disorders. 1. screening of 84 plants against enterobacteria. | gastrointestinal disorders are important causes of morbidity in developing countries. natural healing is the traditional way of treating these diseases in guatemala. ethnobotanical surveys and literature reviews showed that 385 plants from 95 families are used in guatemala for the treatment of gastrointestinal disorders. the activity of 84 of the most commonly used plants was screened in vitro against five enterobacteria pathogenic to man (enteropathogenic escherichia coli, salmonella enteritidi ... | 1990 | 2214824 |
| new approach to develop a vaccine design against shigellosis. | 1990 | 2204244 | |
| recent advances in understanding the pathogenesis of the hemolytic uremic syndromes. | one of the requirements for an agent to cause hemolytic uremic syndrome (hus) is its ability to injure endothelial cells. shiga-like toxin (slt) can do this. slt is produced by escherichia coli and shigella dysenteriae serotype 1; both have been implicated as causes of typical hus. endothelial cells have receptors (gb3) for slt and the toxin can inhibit eukaryotic protein synthesis, thereby causing cell death. glomerular endothelial cell injury or death results in a decreased glomerular filtrati ... | 1990 | 2205273 |
| [a case of shigellosis due to shigella dysenteriae 1 which was thought to be infected in domestic area]. | a 43-year-old man was admitted to our hospital because of bloody diarrhea and abdominal pain on january 10, 1989. on laboratory examination s. dysenteriae 1 was isolated from his stool, therefore he was diagnosed as shigellosis. after the administration of ofloxacin was started, s. dysenteriae 1 was immediately eradicated. but his diarrhea persisted until the 19th day from the onset of the illness and his abdominal pain persisted until the 21st day. he had never been overseas and he had never ea ... | 1990 | 2205674 |
| [detection of two shiga-like toxins from escherichia coli o157:h7 isolates by the polymerase chain reaction method]. | fourteen isolates of e. coli o157:h7 and five isolates of s. dysenteriae type-1 were examined by polymerase chain reaction (pcr) for the structural genes (slt-i or slt-ii), encoding shiga-like toxins (slts). the two primer pairs (v1; 5'agttaatgtggtggcgaa and v2; 5'gactgcgtcagtgaggtt for slt-i, v3; 5'ttcggtatcctattcccg and v4; 5'tctctggtcattgtatta for slt-ii) used were of the same positions representing the dna sequence covering 471bp of the slt-i or slt-ii. a 5-microliter portion of boiled bacte ... | 1990 | 2205737 |
| [recurrent thromboembolism disclosing protein c deficiency. apropos of a case with familial investigation]. | every thromboembolic manifestation, especially in young subjects, calls for an aetiological study in which haemostasis is evaluated primarily with assays of physiological coagulation inhibitors: protein c, protein s and antithrombin iii. protein c deficiency is found in 6 to 7% of thromboembolic manifestations. we report the case of a 21-year old man who had phlebitis followed by pulmonary embolism without facilitating factors. protein c level was 50% of normal value (0.50 iu/ml). the patient re ... | 1990 | 2075365 |
| [the drug resistance and dna plasmid profiles of shigella dysenteriae 1 in the ussr and abroad]. | the medicinal resistance and plasmid profiles of 62 s. dysenteriae strains 1, isolated in the ussr in 1986-1988 from soviet and foreign citizens (from afghanistan, vietnam) and 8 strains obtained from india in 1987 were studied. pronounced similarity between the phenotypes of medicinal, including conjugative, resistance in the strains of soviet and foreign origin was established. in the soviet s. dysenteriae strains 1 the presence of two main types of plasmid dna profiles (140, 6, 4, 2 md and 14 ... | 1990 | 2075761 |
| [effect of maternal virus-specific t-lymphocytes on pregnancy and fetal pathogenesis in experimental congenital influenza infection]. | inoculation of virus-specific cytotoxic lymphocytes (ctl) to pregnant mice infected with influenza virus was shown to result in a decrease of infectious virus concentration in the lungs and blood of mice as well as in the placentas and fetuses. with an increase in the dose of inoculated ctl, however, premature deliveries with stillbirths were observed as well as deaths of the mice, the highest frequency of fetal deaths being observed after infection of pregnant mice in the first half of pregnanc ... | 1990 | 2082546 |
| shiga-like toxin and haemolytic-uraemia syndrome. | 1990 | 2080009 | |
| the mechanism of inhibition of s-adenosyl-l-homocysteine hydrolase by fluorine-containing adenosine analogs. | (z)-4',5'-didehydro-5'-deoxy-5'-fluoroadenosine (i), 5'-deoxy-5'-difluoroadenosine (ii), and 4',5'-didehydro-5'-deoxy-5'-fluoroarabinosyl-adenosine (iii) are inhibitors of rat liver s-adenosyl-l-homocysteine hydrolase. compounds i and ii are time-dependent and irreversible inhibitors of the enzyme. both i and ii are oxidized by e.nad to produce e.nadh, and fluoride anion is formed in the inactivation reaction (0.7 to 1.0 mole fluoride/mole of enzyme subunit, and 1.7 moles fluoride/mole of enzyme ... | 1990 | 2094766 |
| neutralization of poliovirus by cell receptors expressed in insect cells. | to examine the interaction of the poliovirus receptor (pvr) with virus and the role of the pvr in virus entry, the pvr was expressed in insect cells. poliovirus bound to insect cells infected with a recombinant baculovirus (acpvr) carrying cdna encoding the pvr. antibodies raised against pvr expressed in bacteria immunoprecipitated a 67-kilodalton polypeptide from cytoplasmic extracts of acpvr-infected cells. treatment of acpvr-infected cells with tunicamycin revealed that the pvr is a glycoprot ... | 1990 | 2168959 |
| distribution of insertion sequence is200 in salmonella and shigella. | two dna probes for the detection of insertion sequence is200 by either southern blotting or colony hybridization were constructed. one of the probes is a 300 bp ecori-hindiii fragment of is200 cloned onto pbluescript ks(+); the other is a tail-to-tail dimer of the same fragment cloned onto puc19. a survey of the presence of is200 among enteric bacteria revealed that more than 90% of the pathogenic or food-poisoning isolates of salmonella spp. examined contained one or more copies of insertion se ... | 1990 | 1964174 |
| transient cytokeratin expression in skeletal muscle during murine embryogenesis. | cytokeratin expression was investigated in paravertebral skeletal musculature of 10 d and 18 d old embryos as well as in adult nmri-mice. the muscular nature of the evaluated tissue was evidenced by their expression of vimentin and desmin. binding moieties for cytokeratin antibodies (polyclonal and monoclonal) could be demonstrated only in muscle cells of 10 d old embryos. concerning subtypes, the mouse equivalents of the human cytokeratins nos. 8, 18 and 19 could be made probable. the importanc ... | 1990 | 1698345 |
| role of plasmids in virulence-associated attributes and in o-antigen expression in shigella dysenteriae type 1 strains. | the association of plasmids with virulence characters and o-antigen expression was studied in two virulent and seven avirulent mutant strains of shigella dysenteriae type 1. deletion of a 12-mda segment from a 140-mda plasmid in two smooth avirulent mutants made the derivatives avirulent in the sereny test and non-invasive in hela cells. the mutants were unable to bind congo red, and did not express the virulence marker antigen. mutants completely lacking the 140-mda plasmid also showed similar ... | 1990 | 1700125 |
| antigenic conservation of primary structural regions of s-adenosylmethionine synthetase. | although the physical and kinetic properties of s-adenosylmethionine (adomet) synthetases from different sources are quite different, it appears that these enzymes have structurally or antigenically conserved regions as demonstrated by studies with adomet synthetase specific antibodies. polyclonal anti-human lymphocyte adomet synthetase crossreacted with enzyme from rat liver (beta isozyme), escherichia coli and yeast. in addition, polyclonal anti-e. coli enzyme and antibodies to synthetic pepti ... | 1990 | 1698095 |
| characterization of shigella dysenteriae serotypes 11, 12, and 13. | we conducted serologic and biochemical studies on strains of three provisional shigella serotypes. included were 19 strains of serotype 3873-50 from three countries (1 from mexico, 1 from netherlands antilles, and 17 from the united states), 13 strains of serotype 3341-55 from three countries (1 from canada, 1 from bulgaria, and 11 from the united states), and 19 strains of serotype 19809-73 from two countries (16 from israel and 3 from the united states). reactions of these strains with homolog ... | 1990 | 1701447 |
| h(+)-stimulated release of prostaglandin e2 and cyclic adenosine 3',5'-monophosphoric acid and their relationship to bone resorption in neonatal mouse calvaria cultures. | the addition of protons to the medium of neonatal mouse calvaria cultures stimulated bone resorption and release of calcium into the medium. in addition, added protons significantly increased the release of prostaglandin e2 (pge2) and cyclic adenosine 3',5'-monophosphoric acid (camp) from the bones. indomethacin significantly inhibited the release of calcium, pge2 and camp from proton-treated cultures. the positive control, parathyroid hormone (pth)-treated cultures, also gave rise to bone resor ... | 1990 | 1964815 |
| haematological investigation in neonates. | 1990 | 2271791 | |
| effect of the canadian air force training programme on a submaximal exercise test. | validation of the submaximal heart rate/oxygen consumption relationship as an index of 'cardiorespiratory fitness' requires the demonstration of systematic alterations in this relationship concomitant with interventions designed to alter physical fitness. to fulfil those criteria a longitudinal training/de-training study was undertaken. previously sedentary adult subjects undertook the canadian airforce 5bx-xbx exercise programme. submaximal exercise tests were performed before and after trainin ... | 1990 | 258828 |
| the physiological and psychological effects of the expression and inhibition of emotion. | previous work has indicated that humor enhances immunity, but the immunological effects of overt crying have not been studied. thirty-nine women viewed sad and humorous videotapes and either inhibited or expressed overt expressions of laughter and weeping; these were observed and timed. moods and immunity (s-iga) were repeatedly measured. the humorous stimulus resulted in improved immunity, regardless of the overt laughter expressed; overt crying was immunosuppressive, whereas the inhibition of ... | 1990 | 2271804 |
| our brazilian adventure. | 1990 | 269659 | |
| [genetic manipulation research at department of molecular biology university of edinburgh (author's transl)]. | 1990 | 267957 | |
| control of growth of benzo(a)pyrene-transformed 3t3 cells. | the growth controls observed in benzo[a]pyrene-transformed 3t3 cells (bp3t3) are compared with those of virus-transformed and normal 3t3 cells. superficially, the chemically transformed bp3t3 cells have the same behavior as virus-transformed sv3t3 cells. both grow to high cell density in culture medium with 10% serum, both form colonies in methocel, and both are tumorigenic. closer examination, however, has disclosed that bp3t3 cells exhibit "normal" growth controls at low serum concentrations. ... | 1990 | 184467 |
| [correlation between physical load, sympathicus activity and prostaglandins of groups a + e and f in healthy individuals (author's transl)]. | 1990 | 213194 | |
| reaction of the nitroxyl radical tan with glutathione. | 1990 | 213396 | |
| postoperative scars and cleavage lines. | we elucidated the pattern of skin cleavage lines of the neck of japanese cadavers and studied the scarring in 42 cases of radical neck dissection for the removal of metastatic nodules from the malignant tumors of the oral region, in the operation of which one incision was made parallel to, and another perpendicular to, the cleavage lines. from this study we came to the following conclusions: 1) the cleavage lines run parallel in ring-form around the neck, with the exception of the back and the l ... | 1989 | 267516 |
| primary aldosteronism-recognition and management. | 1989 | 267288 | |
| [estrogen therapy and carcinoma of the endometrium (author's transl)]. | the public discussion and the discussion among gynaecologists about the possible increased risk to develop carcinoma of the endometrium following post menopausal treatment with estrogens has lead to insecurity and restricted attitudes in the prescriptions of estrogens. in a statistic comparison of matched pairs of 130 cases of carcinoma of the endometrium with an equal number of selected control cases the following findings were elucidated: 1. any estrogen preparations do not increase the relati ... | 1989 | 210085 |
| possible torsade in a patient with aids. | 1989 | 2115530 | |
| relationship of oral complications to peripheral blood leukocyte and platelet counts in patients receiving cancer chemotherapy. | patients undergoing cancer chemotherapy often suffer from oral complications as a result of their disease and its treatment. the effects of the chemotherapy on the bone marrow and oral mucosa, coupled with the patient's immunosuppressed state and altered oral microbial flora, predispose these patients to oral mucositis, infection, and hemorrhage. the oral mucosa appears to mirror the effects of the chemotherapy on the bone marrow, as there appears to be a direct relationship between the changing ... | 1989 | 313547 |
| occlusal and craniofacial growth changes during puberty. | 1989 | 267438 | |
| production and characterisation of monoclonal antibodies to verotoxins 1 and 2 from escherichia coli of serotype o 157:h7. | fourteen hybridoma cell lines were isolated that produced monoclonal antibodies (mabs) to purified verotoxins 1 and 2 (vt1, vt2) of escherichia coli of serotype o157:h7. of these mabs, eight were obtained by immunisation of balb/c mice with purified vt1, and six were obtained from balb/c mice immunised with purified vt2. with the exception of mab 1c5, with a heavy chain of igg2b class, antibodies produced from mice immunised with heat-treated toxin were of igm class. mabs produced from mice immu ... | 1989 | 2479749 |
| [serological diagnosis of campylobacter jejuni infections]. | antibody level to campylobacter in 28 sera of patients of whom campylobacter infection was confirmed by germ isolation from feces was tested. the investigation was performed using passive haemagglutination technique and as antigens heated and acid glycine extraction prepared from homologous and reference strains. for the method used the heated antigen proved to be superior. out of 28 tested patients of whom 92.8% were children, 8 sera were positive, 9 doubtful and 9, derived mainly from neonates ... | 1989 | 2640683 |
| detection of enterotoxigenic escherichia coli after polymerase chain reaction amplification with a thermostable dna polymerase. | the direct identification of enterotoxigenic escherichia coli from clinical specimens was examined by using the polymerase chain reaction (pcr) for amplifying the heat-labile toxin (lt) gene. two synthetic primers, each of which was 20 bases in length, were used with the thermostable dna polymerase from thermus aquaticus to amplify the lt gene. the amplified pcr products were detected by either gel electrophoresis or hybridization to a 24-base synthetic oligonucleotide probe conjugated to alkali ... | 1989 | 2644292 |
| effect of detoxification processes on the interferon-inducing activity of bacterial endotoxins. | the interferon (ifn)-inducing activity of detoxified lipopolysaccharide (lps) was tested in rabbits treated with lps preparation derived from escherichia coli, salmonella typhi, salmonella enteritidis and shigella dysenteriae serovar 1. of the detoxification procedures used, alkaline hydrolysis, hydroxylaminolysis, formalization, treatment with sodium deoxycholate and the radiodetoxification (fast or slow) methods had no appreciable effects on the ifn-inducing potential of lps. in contrast, acet ... | 1989 | 2484274 |
| studies on shiga-like toxin produced by enterohemorrhagic escherichia coli: purification and characterization of the toxin and development of methods for identifying the toxin. | a simple purification method using deae cellulose column chromatography and immunoaffinity column chromatography was developed for purifying shiga-like toxin produced by escherichia coli o157:h7. about 0.75 mg of purified toxin was obtained from 5 liters of culture (62% recovery). the purified toxin was demonstrated to be immunologically, biologically and structurally indistinguishable from shiga toxin. a sandwich enzyme-linked immunosorbent assay (elisa) was developed for detection of shiga-lik ... | 1989 | 2485395 |
| nonrandom rearrangement of chromosome 14 at band q32.33 in human lymphoid malignancies with mature b-cell phenotype. | chromosomes were studied in 61 patients with differentiated b-cell malignancies including 21 with non-hodgkin's lymphoma (nhl), three with hairy cell leukemia (hcl), eight with waldenström's macroglobulinemia (wm), and 29 with plasma cell disorder. chromosomally abnormal clones were identified in 35 of 61 patients studied: all with nhl, all with hcl, three of eight with wm, and eight of 29 with plasma cell disorder. the most recurrent chromosomal abnormality, observed in 26 of the 35 patients wh ... | 1989 | 2492904 |
| four new provisional serovars of shigella. | four bacterial strains are described that possess the biochemical characteristics of shigella species but do not belong to any of the established shigella serovars or to any previously described provisional serovar. one strain fermented mannitol, and it is proposed that this be the type strain for a new provisional serovar of shigella boydii. the remaining strains did not ferment mannitol and belonged to three different serovars. these strains are proposed as type strains for three new provision ... | 1989 | 2501349 |
| hemagglutinating properties of shigella dysenteriae type 1 and other shigella species. | strains of shigella dysenteriae type 1 cultured in casamino acids-yeast extract broth medium in the presence of 1 mm calcium chloride at 37 degrees c for 22 h induced hemagglutination of erythrocytes that was inhibited by n-acetylneuraminic acid, n-acetylneuramin-lactose, and alpha 1-glycoprotein. the hemagglutination was heat labile, and the absence of cell-surface appendages suggested a nonfimbrial adhesin(s). under the same conditions, strains of shigella flexneri (types 1a, 1b, 2a, and 2b) s ... | 1989 | 2503449 |
| [increased efficiency in the disinfection of sewage by lactic acid bacteria]. | the antimicrobic properties have been studied in 30 strains of lactobacilli. as a result a strain, the strongest antagonist relative to choleric vibrios and other enteropathogenic microorganisms, is selected. lactobacilli are found to retain their viability and biological activity in the activated sludge during the whole period of observation (6 months). biological disinfection of sewage is shown possible to be intensified using the activated sludge inoculated by the culture of the selected lact ... | 1989 | 2514340 |
| identification of shiga-like toxin in escherichia coli strains, etiological agents of diarrheal disease. | shiga-like toxin presence, in 20 e. coli strains, etiological agents of diarrheal diseases, is studied by preparing extracts at +4 degrees c, in the presence of chloroform and by i.v. inoculation in mice. in 4 out of 20 strains, shiga-like toxin in high titres was identified. most of the strains presented an inconstant and variable production of shiga-like toxin in comparison with shigella dysenteriae type 1 (shiga) reference strain. the authors also confirm the existence of shiga-like toxin und ... | 1989 | 2520669 |
| multiple cooperative interactions constrain bpv-1 e2 dependent activation of transcription. | transcription directed by the bpv-1 long control region (lcr) is conditional upon activation by the virally encoded e2 protein. within the 1.0 kb lcr there are five separate regions, a to e, that contain e2 responsive enhancers. the smallest functional region, a, is only 38 bp and contains two copies of the consensus sequence acc(n)6ggt that is known to function as an e2 binding site in vitro. we show that a pair of these constitutes a minimal functional e2 responsive enhancer element but that t ... | 1989 | 2542891 |
| dual function of a nuclear factor i binding site in mmtv transcription regulation. | using linker-scanning mutagenesis we had previously identified four elements within the mmtv ltr which are necessary for transcriptional stimulation by glucocorticoid hormones. two of them overlapped with regions to which the glucocorticoid receptor binds in vitro. the third element contained a nf-i binding site, and the fourth the tata box. here we show that mutations that abolish in vitro binding of nf-i had a negative effect also on the basal activity of the mmtv promoter of ltr-containing pl ... | 1989 | 2542892 |
| expression and desensitisation of a2 purinoceptors on cultured bovine aortic endothelial cells. | purine nucleotides and their metabolites are important mediators of vascular tone. adenosine promotes relaxation of vascular smooth muscle, acting on the a2 subclass of purinoceptors. there is some evidence that these receptors are also present on vascular endothelial cells. we have therefore examined cultured aortic endothelial cells for adenosine (a2) sensitivity. bovine aortic endothelial cells (ag4762) were obtained from the institute of aging cell repository (usa), and cultured in monolayer ... | 1989 | 2590913 |
| [in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics]. | in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ... | 1989 | 2518646 |
| influence of diethyldithiocarbamate on nickel-induced hepatic metallothionein in rats. | the influence of sodium diethyldithiocarbamate (ddc) on 63nickel chloride-induced metallothionein (mt) and alterations in essential metal levels in the liver of rats was investigated. the induction of mt, zn and cu levels of the hepatic cytosolic "heat stable fraction" (hsf) by ddc increased with time up to 24 hr. although mt, zn and cu were significantly greater at 17 hr than those at 6.5 hr after 63ni administration, the ni level decreased. the treatment with ddc at 6 hr, but not at 10 min, pr ... | 1989 | 2548306 |
| endocytosis from coated pits of shiga toxin: a glycolipid-binding protein from shigella dysenteriae 1. | evidence is presented that endocytosis is involved in the transport to the cytosol of the cytotoxin from shigella dysenteriae 1, shiga toxin, which acts by removal of a single adenine residue in 28-s ribosomal rna. inhibition of endocytosis by atp depletion of the cells prevented toxin uptake. exposure of hela s3 and vero cells to toxin at low extracellular ph, where translocation to the cytosol, but not endocytosis is inhibited, allowed the toxin to accumulate in a compartment where it was prot ... | 1989 | 2564398 |