Publications

functional role of hla class i cell-surface molecules in human t-lymphocyte activation and proliferation.	this investigation addressed the role of major histocompatibility complex-encoded class i molecules in the activation and proliferation of human lymphocytes. we studied the effect of antibodies specific for hla-a and hla-b locus gene products on mitogen-stimulated peripheral blood mononuclear cell (pbmc) subpopulations. three individually derived, well-characterized anti-hla class i monoclonal antibodies were demonstrated to inhibit the proliferation of human pbmc stimulated by either okt3 or th ...	1986	3012568
adult t-cell leukemia/lymphoma not associated with human t-cell leukemia virus type i.	we describe five patients with adult t-cell leukemia/lymphoma (atl) with neither integration of human t-cell leukemia virus type i (htlv-i) into their leukemia cells nor anti-htlv-i antibody in their sera. these findings indicate that htlv-i may not have been involved in leukemogenesis in these patients. the clinicohematological, cytopathological, and immunological features of htlv-i-negative atl were exactly the same as those of htlv-i-associated atl. leukemia cells with pleomorphic nuclei, gen ...	1986	3012571
[t4 polynucleotide kinase].		1986	3012647
lymphoreticular infiltrates in invasive ductal breast cancer. a histological and immunohistological study.	fifty-two invasive ductal breast cancers were investigated histologically and immunohistologically to assess localization and composition of the lymphoreticular infiltrates. the tumour-infiltrating cells were mainly located in the intervening stroma, whereas tumour foci often exhibited lower numbers of lymphoreticular cells. macrophages (mono 1+ and kim 6+) and helper/inducer cells bearing the t4 surface antigen (leu-3a+) regularly constituted the majority of the tumour-infiltrating lymphoreticu ...	1986	3012862
relationship of exposure to human t lymphotropic virus and t helper cells in homosexual men.	the detection of serum antibodies (ab) against htlv-iii in individuals with aids and related symptoms (arc) has unambiguously defined the association of the virus infection to aids. this study was done to determine the extent of exposure to htlv-iii in homosexual men by measuring (ab) and relating it to the stage of disease and t cell subsets. we found ab in 89.5% of the 492 men with the median titers by stage of disease being 1600 for symptom-free, 6400 for arc or kaposi's sarcoma, and 4800 for ...	1986	3013220
antibodies to aids-associated retrovirus (htlv-iii/lav) in drug addicts from vizcaya, northern spain.	serum samples from 313 asymptomatic intravenous (iv) drug users from bilbao (vizcaya, vasque country, spain) were tested for antibodies to htlv-iii/lav virus, the probable etiologic agent of the acquired immune deficiency syndrome (aids). viral antibodies were assayed by elisa test. 41.9% of the sera gave positive reactions. no seropositivity was detected among 22 normal blood donors, 58 chronic alcoholics, or 20 members of the drug control center personnel. virus specific reactions were confirm ...	1986	3013221
[value of the cytological examination of the bronchoalveolar lavage fluid in patients with acquired immunodeficiency syndrome and related syndromes].	in aids a variety of severe pulmonary disorders may occur. the authors report 110 cases of bronchoalveolar lavage (bal) in 43 aids and 41 arc. in aids p. carinii pneumonia is the major cause of respiratory illness. bal alone is a safe and valuable tool for diagnosis of p. carinii pneumonia and others opportunistic infections. moreover, pulmonary hemorrhage diagnosed by the finding of hemosiderin laden macrophages, is very suggestive of broncho-pulmonary kaposi' sarcoma. finally, bal demonstrates ...	1986	3013225
human t lymphotropic virus type iii infection of human alveolar macrophages.	the human t-cell lymphotropic virus type iii (htlv-iii) is the etiologic agent of the acquired immunodeficiency syndrome (aids) and preferentially infects t4 lymphocytes. other cell types, notably b lymphocytes and other nonlymphoid cells, also have been reported to be infected in vitro by htlv-iii. we now report on the susceptibility of human pulmonary macrophages to infection with htlv-iii in vitro. alveolar macrophages infected with htlv-iii produced low levels of virus that could be transfer ...	1986	3013342
natural history of primary infection with lav in multitransfused patients. by the aids-hemophilia french study group.	in the course of a prospective study of asymptomatic, multitransfused subjects, seroconversion to human lymphadenopathy-associated virus (lav/htlv-iii) occurred in 34 hemophilic and in two thalassemic patients. in subjects treated with procoagulant concentrates, primary infection, as evidenced by the development of antibodies to lav, was a clinically silent event apart from moderate lymph node enlargement in 21% of cases. concomitant immunologic disturbances mainly affected t lymphocyte subsets. ...	1986	3013344
[lav type ii: a second retrovirus associated with aids in west africa].	a second retrovirus, named lav-ii, has been isolated from two west-african patients with aids. by its genomic sequences and its proteins, this virus is different from the lav-i/htlv-iii, isolated from u.s.a., europe and central africa. it differs also from stlv-iii, isolated from rhesus macaques with aids, but displays an antigenic relationship with the latter virus, at the level of its external envelope protein. the tropism of lav-ii for t4 lymphocytes and the induction of cytopathic effect in ...	1986	3013382
suppression of concanavalin a-induced blastogenesis by htlv-iii-infected h9 cells.	the ability of cells infected with human t lymphotropic virus type iii (htlv-iii) to suppress lymphocyte responses to concanavalin a (cona) was evaluated. thirty homosexual men, both htlv-iii seropositive and seronegative, and 11 seronegative laboratory personnel were studied. peripheral blood lymphocytes from all groups had lower responses to cona in the presence of htlv-iii-infected h9 cells than in the presence of uninfected h9 cells. among htlv-iii-seropositive males, responses to cona in th ...	1986	3013473
binding of thyroid hormones to nuclear extracts of thyroid cells.	binding of t3 and t4 to soluble nuclear extracts of frtl-5 cells, rabbit thyroid glands, and rat liver was studied. [125i]iodo-t3 or [125i]iodo-t4 in concentration ranges of 100-fold (10-fold on each side of measured kd) was incubated with extract at 4 c, ph 8.2, and the quantity of bound hormone was determined by collection on nitrocellulose filters. the results were corrected for nonspecific binding. steady state (equilibrium) binding was achieved by 36 h. apparent dissociation constants (kd) ...	1986	3013592
differentiation of human mature thymocytes: existence of a t3+4-8- intermediate stage.	a t3 complex-bearing subpopulation was characterized within an in vivo cycling t4-8- early thymocyte compartment which contains cells constitutively expressing interleukin 2 and transferrin receptors. we show differentiation in vitro of both mature subsets of thymocytes (t3+4+8- and t3+4-8+) from the above t4-8- compartment, their appearance being preceded by cells in a t3+4-8- intermediate stage. furthermore, those mature thymocytes generated in vitro contain functionally competent cells which ...	1986	3013640
triggering of the lethal hit in human cytotoxic t lymphocytes: a functional role for a 103-kda t cell-specific activation antigen.	a monoclonal antibody (cb.1) is described that defines a new triggering signal for human cytotoxic t lymphocytes (ctl). the antibody precipitates a 103-kda surface antigen from activated normal human t cells. the antigen is undetectable or present in only low amounts on resting t lymphocytes but its expression increases strongly after activation and proliferation on t4+ and t8+ t lymphocytes. binding of antibody cb.1 to ctl results in triggering of the lethal hit. this induction of cytotoxicity ...	1986	3013644
discrete stages of human thymocyte activation and maturation in vitro: correlation between phenotype and function.	using light scatter, flow cytometric and functional analyses, three major stages in the in vitro activation of human thymocytes were defined. the earliest stage (day 0-2) was characterized by the induction of the interleukin 2 (il2) and transferrin receptors, the t cell lineage specific ta1 antigen, and increased reactivity with anti-t8 antibody. at this time, major changes in nuclear morphology but not cell size were observed. early-stage thymocytes were immature with regard to t3 and cytotoxic ...	1986	3013646
subcellular localization and binding of 125i-triiodothyronine in calf thyroid.	the subcellular distribution of 125i-t3 was studied in calf thyroid slices, under the same experimental conditions where t3 inhibits protein and rna synthesis, labelled hormone was found mainly in the 20,000 x g supernatant. the specificity of each subcellular localization was determined by incubating the slices with 10(-5)m t3. only in the purified nuclei a significant decrease was found, indicating a specific localization of the labelled hormone. when slices were incubated with 125i both label ...	1986	3013744
differential expression of ecto-5' nucleotidase activity by functionally and phenotypically distinct subpopulations of human leu-2+/t8+ lymphocytes.	subsets of leu-2+/t8+ cytotoxic/suppressor t lymphocytes were isolated by using various methods of purification and were investigated for expression of ecto-5' nucleotidase (5'nt) enzyme activity by radiochemical, cytochemical, and ultrastructural techniques. by using both the radiochemical and the cytochemical methods. t4-okm1- cells displayed higher 5'nt activity in comparison with the entire t4- subpopulation. analyses of the subpopulations of t4- (and predominantly leu-2+) cells defined by t ...	1986	3013991
prevalence of htlv-iii/lav antibodies in italian asymptomatic hemophiliacs given commercial concentrates of factors viii and ix.	the prevalence of htlv-iii/lav antibodies was evaluated in 222 italian asymptomatic hemophiliacs treated exclusively with commercial factor concentrates made with american plasma. an increase of htlv-iii/lav seropositivity from 1983 to 1985 was evident. this was independent of the type of hemophilia (a or b) but directly correlated to the amount of concentrate administered in the previous year. immunological data (t4 and t8 lymphocyte subsets) were evaluated in relation to seropositivity to htlv ...	1986	3014059
characterization of a defective phage system for the analysis of bacteriophage t4 dna replication origins.	we have developed a defective phage system for the isolation and analysis of phage t4 replication origins based on the t4-mediated transduction of plasmid pbr322. during the initial infection of a plasmid-containing cell, recombinant plasmids with t4 dna inserts are converted into fully modified linear dna concatamers that are packaged into t4 phage particles, to create defective phage (transducing particles). in order to select t4 replication origins from genomic libraries of t4 sequences clone ...	1986	3014155
transmissible lymphoma and simian acquired immunodeficiency syndrome in rhesus monkeys.	four rhesus monkeys (macaca mulatta) were inoculated with a homogenate of a cutaneous lepromatous leprosy lesion from a mangabey monkey (cercocebus atys). one died of b-cell lymphoma, and another died of an immunodeficiency syndrome. cell suspensions prepared from the tumor and spleen of the monkey with lymphoma induced lymphoma or an immunodeficiency syndrome when inoculated into additional young rhesus monkeys. the immunodeficiency syndrome was similar to simian acquired immunodeficiency syndr ...	1986	3014195
[effect of superhelical dna on the transcription of cloned genes of the t4 phage].	the effect of dna superhelicity on the transcription of t4 dna fragments containing early genes uvs w, y and late genes 25-29 was studied. rna polymerase transcribes both early and late phage genes within the supercoiled recombinant plasmid. late genes relative transcription increases essentially when t4-modified rna polymerase is used. dna relaxation causes a sharp decrease of modified rna polymerase activity, especially on the late genes. the same effect is obtained in intact cells with recomb ...	1986	3014315
microinjection of escherichia coli uvra, b, c and d proteins into fibroblasts of xeroderma pigmentosum complementation groups a and c does not result in restoration of uv-induced unscheduled dna synthesis.	the uv-induced unscheduled dna synthesis (uds) in cultured human fibroblasts of repair-deficient xeroderma pigmentosum complementation groups a and c was assayed after injection of identical activities of either uvr excinuclease (uvra, b, c and d) from escherichia coli or endonuclease v from phage t4. under conditions where the t4 enzyme was able to induce repair synthesis in both xp complementation groups in agreement with earlier observations (de jonge et al., 1985), no effect of the uvrabcd e ...	1986	3014326
twenty-four-hour serum levels of t4 and t3 in relation to decreased tsh serum levels and decreased tsh response to trh in affective disorders.	the serum levels of thyroxine and triiodothyronine (t4 and t3) were investigated at 10 different time points during a 24 h period in 31 inpatients meeting the rdc criteria for acute major depressive disorder. twenty-three of these patients were also reinvestigated in a state of partial or complete remission. the results show that there was no significant difference in t4 or t3 levels during the 24 h period between depressed patients and 32 healthy controls despite significantly decreased tsh lev ...	1986	3014823
[neuroendocrine response to anesthesia with isoflurane].	the effects during surgery of a new halogenated volatile anaesthetic, isoflurane, on the hypothalamo-hypophyseal-thyroid-suprarenal axis were studied. in fact, it was important to prove whether this new halogenated anaesthetic would provide better protection, for the patient, from surgery and anaesthetic stress compared with other anaesthetic agents in use. the study was carried out in 16 young class asa i patients who were to undergo appendicectomy. before and during operation, blood was taken ...	1986	3014925
activated alveolar macrophage and lymphocyte alveolitis in extrathoracic sarcoidosis without radiological mediastinopulmonary involvement.	cellular characteristics of bal were investigated in 18 patients with proved extrathoracic sarcoidosis (that is, sarcoidosis that affected the skin, eyes, parotid glands, stomach, nose, kidneys, or meninges) without clinical or radiological mediastinopulmonary involvement. computed tomography of the thorax was performed on five patients: four patients were normal, and one had enlarged lymph nodes (these enlargements were not detectable on the patient's chest roentgenogram). the results of pulmon ...	1986	3014953
the stimulation of angiotensin-converting enzyme induction in cultured monocytes by t4+ and t8+ lymphocytes.		1986	3014959
human t-cell lymphotropic virus type iii associated disorders. the spectrum in the heterosexual population.	eleven heterosexual patients (nine women, two men) without classic risk factors for development of acquired immunodeficiency syndrome (aids) were seen between march 1983, and april 1985, and diagnosed as having aids (four), persistent, generalized lymphadenopathy (pgl) (four), or asymptomatic human t-cell lymphotropic virus type iii (htlv-iii) carrier state (three). the clinical presentations and course of those with aids or pgl were similar to those reported in homosexual men with aids or pgl, ...	1986	3015064
comparison of the effects of pancuronium and tubocurarine on different muscles of young and old mice.	in order to evaluate the sensitivity of different muscle types to neuromuscular blocking drugs, a system using mouse muscles in vitro was developed and applied to detect changes in drug sensitivity in relation to age. the effect of pancuronium and tubocurarine on initial twitch and on the ratio of fourth twitch to first twitch (t4/t1) of a train-of-four at 2 hz were compared in fast-twitch, slow-twitch and respiratory muscles in the mouse. the muscles used were: extensor digitorum longus (edl), ...	1986	3015178
[effect of thyroid hormone receptors in normal and cancerous cells on the ionic conductivity of bilayer phospholipid membranes].	the effect of thyroid hormones receptors isolated from normal and cancer cells on bilayer phospholipid membranes (bphlm) conductivity, has been studied. the receptor isolated from normal cells in complex x with the hormone selectively induces h+-conductivity of bphlm generating transmembrane potential equal to 42 mv on the membrane at ph gradient equal to 1. in the presence of k+, na+, ca+, mn2+, sr2+, mg2+ the changes of bphlm are not observed. neither hormones (t3, t4) nor receptor in free pos ...	1986	3015268
transient immune deficiency in patients with acute epstein-barr virus infection.	to study the effect of primary epstein-barr virus (ebv) infection on antigen-specific antibody production, we immunized 17 college students who had developed acute infectious mononucleosis with the t-cell dependent neoantigen bacteriophage phi x174. during the early phase of infectious mononucleosis, the proportion of peripheral blood lymphocytes displaying ia and t8 (cd8) phenotypes was increased and the t helper/suppressor (t4/t8) ratio was decreased (less than 1). these abnormalities disappea ...	1986	3015461
effects of an acute bacterial infection on serum thyroid hormones and nuclear triiodothyronine receptors in mice.	serum t3, t4, and rt3 levels as well as liver nuclear t3 receptors (nt3r) were measured in mice with a bacterial infection. pseudomonas aeruginosa were injected into one thigh of icr mice, resulting in a severe infection at sacrifice 15 h later. since food intake, which influences serum thyroid hormone levels and nt3r, was 75% lower in infected than in control mice, infected mice were either fed and compared with pair-fed controls or fasted and compared with fasted and fed controls. fasting indu ...	1986	3015549
thyroid hormone regulation of epidermal growth factor receptor levels in mouse mammary glands.	the specific binding of iodinated epidermal growth factor ([125i]iodo-egf) to membranes prepared from the mammary glands and spontaneous breast tumors of euthyroid and hypothyroid mice was measured in order to determine whether thyroid hormones regulate the egf receptor levels in vivo. membranes from hypothyroid mammary glands of mice at various developmental ages bound 50-65% less egf than those of age-matched euthyroid controls. treatment of hypothyroid mice with l-t4 before killing restored b ...	1986	3015551
effects of norepinephrine on basal and thyrotropin-stimulated thyroid hormone secretion in the mouse.	the possibility that norepinephrine stimulates basal but inhibits tsh-induced thyroid hormone secretion was explored by the use of in vivo and in vitro techniques. basal thyroid hormone secretion was defined as tsh-independent secretion, experimentally obtained in vivo by pretreatment with t4 or t3. in mice pretreated with 125i and t4, norepinephrine enhanced baseline blood radioiodine levels, but in control mice, norepinephrine failed to alter basal plasma t4 levels. to study whether the stimul ...	1986	3015564
effect of the antileukemic agent l-asparaginase on thyroxine-binding globulin and albumin synthesis in cultured human hepatoma (hep g2) cells.	l-asparaginase (asnase), a drug widely used in the treatment of acute lymphoblastic leukemia, has been reported to decrease serum t4-binding globulin (tbg) levels, while results of serum albumin determinations were conflicting. this effect in vivo has been attributed to depressed liver protein synthesis, but this hypothesis has not been proved. to investigate this problem, human hepatoma (hep g2) cells were continuously labeled for 4 h with 100 microci/ml [35s]methionine in the absence or presen ...	1986	3015570
increase in the number of type ii insulin-like growth factor receptors during propylthiouracil-induced hyperplasia in the rat thyroid.	we have observed that membranes isolated from rat thyroids contain receptors for the insulin-like growth factors (igf). as igfs are known to be important mediators of tissue growth, we conducted this study to determine whether modulation of thyroid igf receptors might be involved in tsh-stimulated hyperplasia. a substantial increase in both the weight of the thyroid and its dna content was observed within 2 days of exposing adult male rats to 0.1% propylthiouracil (ptu) in their drinking water. ...	1986	3015571
seasonal changes in serum thyroid hormone binding proteins in the woodchuck (marmota monax).	to evaluate the role of serum t4- and t3-binding proteins in the elevation of serum t4 and t3 concentrations in the woodchuck in the fall and winter, blood was collected from woodchucks during the four seasons of the year (seasonal study) and from 2-week fasted woodchucks in the summer (fasting study) and the serum concentrations of total t4 and t3 were measured. the distribution of [125i]4 and [125i]t3 tracers among the serum binding proteins and the serum t4-binding globulin (tbg) binding capa ...	1986	3015576
a possible competition between 5'-monodeiodination of thyroxine and the respiratory burst in human granulocytes.	thyroxine (t4) pretreatment of a 23187-stimulated human granulocytes in 10(-5)-10(-6) m concentration range inhibited the superoxide anion production of these cells. t4 increased the level of oxidized form of glutathione, whereas the intracellular level of the reduced form decreased. a similar alteration in the ratio of the oxidized to reduced forms of glutathione was detected in granulocytes during yeast cell phagocytosis. in addition, conversion of t4 to triiodothyronine (t3) was also inhibite ...	1986	3015757
production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.	we constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human t4 cells. the progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-t cell lines, indicating the absence of any intracellular obstacle to viral rna or protein production or assembly. during the course of these studies, a hum ...	1986	3016298
hormonal interactions with benzodiazepine binding sites in vitro.	prostaglandin a1 and hormones like corticosteroids and dl-thyroxin (t4) inhibit binding of [3h]ro 5-4864 and [3h] clonazepam to their respective binding sites with inhibition constants in the low micromolar range. the corticosteroid cortisone inhibits [3h] ro 5-4864, but not [3h] clonazepam binding in a competitive manner with an inhibition constant of 4.3 +/- 0.7 microm, prostaglandin a1 inhibits [3h] clonazepam, but not [3h] ro 5-4864 binding in a competitive manner with an inhibition constant ...	1986	3016447
role of the htlv-iii/lav envelope in syncytium formation and cytopathicity.	acquired immune deficiency syndrome (aids) is characterized by marked depletion of the t4+ helper subset of t cells. the aetiological agent of the disease, the human t-lymphotropic virus type iii (htlv-iii)/lymphadenopathy-associated virus (lav), specifically kills t4+ cells in vitro. part of this specificity for the t4+ population residues in the relative efficiency with which htlv-iii infects these cells, as a result of a specific interaction between the t4 molecule and the virus envelope glyc ...	1986	3016552
mononucleosis-like-syndrome associated with acute aids retrovirus infection.	a 47-year-old man developed an acute infectious mononucleosis-like illness with a moderate lymphocytosis and numerous atypical lymphocytes in the peripheral blood. seroconversion to the aids-associated virus occurred between 14 and 20 days following the onset of the acute illness. he was found to have reduction of the t4:t8 ratio, low mitogenic response to pha and cutaneous anergy when tested at 25 and 136 days. these tests had returned to normal by 262 days.	1986	3016618
[adult t-cell lymphoma/leukemia associated with htlv-i virus in martinique: apropos of 2 cases].	two htlv-i associated adult t cell leukemia cases were observed in patient from martinique (french west indies). these case are similar to the clinical entity, described by takatsuki in 1977 in japan and by catovsky in caribbean patients, characterized by a lymphadenopathy, skin lesions and visceral involvement, hypercalcemia, an aggressive course, and poor prognosis. the malignant cells with t4 phenotype and often suppressive function, were pleomorphic, mature, with prominent nuclear irregulari ...	1986	3016639
[aids physiopathology and epidemiology of the infection with the lav virus].	lav is the causative agent of aids. its biological properties, assessed in vitro, fully account for the major immunological abnormalities which characterize the disease. lav is a lentivirus which presents a selective tropism for t4+ lymphocytes, the very cells that are destroyed in aids. its replication is associated with inhibition of lymphocyte proliferative capacity and with a cytopathic effect. in most instances, lav infection is silent, for years at least, or it can result in various clinic ...	1986	3016830
the clinical importance of leukocyte depletion in regular erythrocyte transfusions.	antilymphocyte, antigranulocyte and antiplatelet alloantibodies, t lymphocyte subsets, expression of hla-dr antigens on t lymphocytes and nk cell function were determined in 11 homozygous beta-thalassemic children multitransfused ab initio with erypur-filtered leukocyte-free red cell units (group a) and in 13 similar children multitransfused with standard packed red cell units (group b). no antibodies were found in group a patients, whereas 69% of group b patients were immunized. the two groups ...	1986	3017002
relation of human t lymphotropic virus type iii antibodies to t lymphocyte subset abnormalities in hemophiliac patients.	the relationship of t lymphocyte subset abnormalities and the presence of antibodies to the human t lymphotropic virus type iii (htlv-iii) was evaluated in 66 adult patients with hemophilia. positive test results for antibodies to htlv-iii were observed in 62 percent of patients with hemophilia a and 9 percent of patients with hemophilia b. patients with htlv-iii antibodies had lower percentages and numbers of t helper (t4) cells and increased percentages of t suppressor (t8) cells compared with ...	1986	3017105
differential expression of sos genes in an e. coli mutant producing unstable lexa protein enhances excision repair but inhibits mutagenesis.	the lexa41 mutant of e. coli is a uv-resistant derivative of another mutant, lexa3, which produces a repressor that is not cleaved following inducing treatments. lexa41 carried an additional mutation which changed amino acid 132 in the lexa protein from ala to thr. the resultant protein was unstable and was degraded both before and after an inducing treatment. this instability was greater at 42 degrees than at 30 degrees. the protein was more stable in lon- mutants at both temperatures. lac oper ...	1986	3017286
restoration of dna repair in uv-sensitive chinese hamster ovary cell by the denv gene from bacteriophage t4.		1986	3017288
a specific model for the conformation of single-stranded polynucleotides in complex with the helix-destabilizing protein gp32 of bacteriophage t4.		1986	3017469
role of adenylate cyclase in human t-lymphocyte surface antigen capping.	our recent studies indicated that capping of t3, t4 and t8 surface antigens on human t lymphocytes is augmented by interaction of adenosine with a purinergic receptor. we suggested that the t-cell capping process was mediated by an adenylate cyclase-coupled purinergic receptor that resulted in the generation of camp and occupancy of camp receptors. the present study was undertaken to examine whether activation of adenylate cyclase in the absence of purinergic stimulation is sufficient to regulat ...	1986	3017571
nuclease p1-mediated enhancement of sensitivity of 32p-postlabeling test for structurally diverse dna adducts.	exceedingly sensitive procedures are required to detect the presence of covalent dna adducts in humans exposed to environmental genotoxicants because of low levels of such derivatives (1 adduct in 10(8)-10(10) dna nucleotides). a 32p-postlabeling assay for detection and quantitation of carcinogen--dna adducts with a sensitivity limit of 1 adduct in 10(7)-10(8) nucleotides has been described previously. in the standard procedure, dna is enzymatically digested to 3'-phosphorylated normal and adduc ...	1986	3017601
modulatory effects of thyroxine treatment on central and peripheral benzodiazepine receptors in the rat.	the effects of 10 days of d-thyroxine (t4) treatment on central benzodiazepine (bz) receptors in the brain and on peripheral-type bz binding sites in the heart, kidney, and testis of rats were studied. the experimental hyperthyroidism resulted in an increase in the density of cortical central bz receptors, without any alteration of the affinity of the receptors to [3h]flunitrazepam. the increase in cortical central bz receptors was also accompanied by the up-regulation of peripheral bz binding s ...	1986	3018159
replication of human cytomegalovirus in human peripheral blood t cells.	the replication of human cytomegalovirus (hcmv) strain ad169 was studied in human peripheral blood granulocytes, monocytes-macrophages, b lymphocytes, and t lymphocytes. progeny virus was produced in some t-cell cultures stimulated in the allogeneic mixed lymphocyte reaction and was regularly obtained when stimulated t cells were grown in the presence of interleukin 2. replication of hcmv in these cultures was documented by increases in titer, expression of early and late antigen as assessed by ...	1986	3018292
topoisomerase ii and other dna-delay and dna-arrest mutations impair bacteriophage t4 dna packaging in vivo and in vitro.	a survey of dna packaging in vivo and in vitro during infections caused by t4 dna-delay and dna-arrest amber mutants revealed a common dna packaging-deficient phenotype. electron microscopy revealed high proportions of proheads partially filled with dna in vivo, indicating normal initiation but incomplete encapsidation. in contrast, exogenous mature t4 dna was packaged in vitro by several early-gene mutant extracts. detailed analysis of gene ts39 mutants (subunit of topoisomerase ii) showed that ...	1986	3018302
nuclear thyroid hormone receptors in cultured human fibroblasts: improved method of isolation, partial characterization, and interaction with chromatin.	in order to characterize the nuclear thyroid hormone receptors in human tissue, an improved method for isolation of nuclei from cultured human fibroblasts was developed. this method provided nuclei with a protein/dna ratio of 2.8 and recovery of 42%. the purity of nuclei was verified by phase contrast and electron microscopy, which showed normal appearance of chromatin structure. nuclear binding assay was performed by incubation of whole cells at 37 degrees c or isolated nuclei at 22 degrees c w ...	1986	3018426
technetium 99m pertechnetate thyroid scintigraphy: congenital hypothyroid screening.	technetium 99m pertechnetate thyroid scans were performed on 57 infants referred for evaluation of suspected congenital hypothyroidism. thyroid anatomy may be characterized by four general types, based on the scintigraphic findings: (1) normal size and location; (2) ectopic location; (3) no detectable thyroid activity; (4) normal location with increased size or uptake. there are diverse etiologies of congenital hypothyroidism. correlation of thyroid scintigraphy with blood t4 and tsh levels allo ...	1986	3018656
nuclear thyroxine binding in human mononuclear blood cells from hyperthyroid and hypothyroid patients before and after treatment.	nuclear binding of [125i]t4 in human mononuclear blood cells was examined in six hyperthyroid and six hypothyroid patients before and after treatment. in hypothyroid patients the nuclear t4 binding was initially increased and subsequently normalized as the patients became euthyroid suggesting a homeostatic counter-regulation. the thyroid state did not affect the nuclear t4 binding in hyperthyroid patients. treatment with methimazol however increased the nuclear t4 binding, suggesting either a di ...	1986	3018913
positive selection of activated t cells of the t8 (cd8) sub-type by immunomagnetic separation.	by conjugating a monoclonal igm antibody of cd8-specificity to magnetite-containing polymer particles, we have developed a rapid and simple one-step procedure for positive selection of t8 cells. the method ensured good yield and viability of pure t8 cells. the positively selected t8-fraction from mlc activated t cells retained their cytolytic capacity and gave a moderate proliferative response. most of the proliferative response occurred in the negatively selected t4 population which showed only ...	1986	3018960
factors in addition to the direct cytopathic action of htlv-3/lav contribute to the loss of t4 cells characteristic of aids.		1986	3019361
differential recognition of tumor-derived and in vitro epstein-barr virus-transformed b-cell lines by fetal calf serum-specific t4-positive cytotoxic t-lymphocyte clones.	two interleukin-2 (il-2)-dependent cytotoxic t-cell clones were obtained by limiting dilution from a lymphocyte culture stimulated in vitro with the autologous epstein-barr virus-transformed lymphoblastoid cell line (lcl) in the presence of fetal calf serum (fcs). both clones uniformly had a t3+, t4+, dr+ phenotype and lysed autologous b blasts, the autologous lcl, and allogeneic b cell lines sharing major histocompatibility complex (mhc) class ii antigens. the cytotoxic function was triggered b ...	1986	3019570
cloning and expression of an intron-deleted phage t4 td gene.	the 1017-bp intron within the cloned phage t4 td gene was deleted by oligonucleotide-directed mutagenesis. induction of thymidylate synthase activity and mature td mrna from this intronless construct (pktd delta i) was compared both in vivo and in vitro with expression from plasmids bearing td genes in which the introns contain either no change (pktd2), an xbai linker inserted about 200 nucleotides from the 3'-end (pktdx-1), or a deletion of two-thirds of the central portion (pktd delta 1-3). sl ...	1986	3020032
t lymphocyte cloning from rejected human kidney allografts. growth frequency and functional/phenotypic analysis.	mechanically harvested lymphocytes invading an irreversibly rejected human kidney allograft were seeded at limiting dilution to calculate the frequency of growing precursors. optimal growth frequency (1/13) was obtained when epstein-barr virus (ebv)-transformed donor b lymphocytes were used as stimulators (d-blcl) in the presence of interleukin 2 (il-2). the 55 clones analyzed were all t11+ and t3+, and all expressed dr antigens (45% were t8+ and 55% t4+). only one clone had a double-labeled (t4 ...	1986	3020092
gonadal dysfunction in patients treated for metastatic germ-cell tumors.	the effects of chemotherapy on endocrine function were assessed in 22 previously treated patients with germ-cell tumors and compared with the endocrine function of six previously untreated patients. baseline and stimulated serum levels of luteinizing hormone (lh), follicle-stimulating hormone (fsh), testosterone, thyroid-stimulating hormone (tsh), prolactin, and thyroxine (t4) were obtained. baseline lh levels were elevated in both groups of patients, whereas basal fsh levels were significantly ...	1986	3020183
[bilateral cytomegalovirus retinitis in an infant with fatal congenital aids].	the authors report on a baby born on august 25, 1984 with a birthweight of 1100 g. her parents used drugs intravenously and her mother had anti-htlv iii antibody. in the clinical course an immune deficiency syndrome with pathologic reduction of t4:t8 (t-helper: t-suppressor cells) was apparent. the child had no anti-htlv iii antibody, but exposition, typical clinical course and immunological data led to the diagnosis acquired immune deficiency syndrome (aids). in the 9th month bilateral progress ...	1986	3020309
immortalization of human t lymphocytes by htlv-i: phenotypic characteristics of target cells and kinetics of virus integration and expression.	in-vitro infection of normal human lymphocytes with htlv-i (human t-cell lymphotropic retrovirus type i) has been carried out to study the target cell specificity and the kinetics of infection. cord blood (cbl) and adult peripheral blood lymphocytes (pbl) have been co-cultivated with irradiated htlv-i donor cells (mt2 and c91pl lines). established ('immortalized') htlv-i positive cell lines were obtained only from cbl: in comparison with pbl, a less mature phenotype of t-cell subsets and a lower ...	1986	3020320
[preoperative staging of rectal cancer with computer tomography].	the correlation of the preoperative staging by ct with the postoperative staging was prospectively investigated in 112 patients with carcinoma of the rectum. the influence of ct on the choice of surgical treatment was also proven. the evaluation of the infiltration of perirectal tissue and especially of other organs is possible with ct. according to tnm classification the pre- and postoperative staging showed identical results by conventional diagnostic methods in t1 in 7/16, in t2 in 22/38, in ...	1986	3020324
immunological reactivity in children with wilms' tumor.	cellular immune reactivity was investigated in 49 newly diagnosed children with wilms' tumor and compared to age-matched control. the level of total t (t4 degrees) and b lymphocytes was normal while the relative number of t lymphocytes with high affinity receptors for sheep erythrocytes (t29 degrees) was significantly decreased in the patients studied. the lymphocyte response to pha in vitro was diminished but pha-induced lymphokine production was not altered. the depression of t29 degrees level ...	1986	3020456
the 52-protein subunit of t4 dna topoisomerase is homologous to the gyra-protein of gyrase.	t4 gene 52 encodes one of the three subunits of t4 dna topoisomerase. the t4 enzyme is required for normal phage dna replication. i have cloned the entire gene, and it is expressed in uninfected e. coli cells. the sequence of 1966 nucleotides of t4 deletion delta sa9 surrounding gene 52 has been determined. the reading frame of the gene was established by identifying the first ten amino acids in the large open reading frame derived from the dna sequence as those at the amino-terminus of the puri ...	1986	3020513
thyroid function after postoperative radiation therapy in patients with breast cancer.	thyroid stimulating hormone (s-tsh) and thyroxine (s-t4) were measured in sera from 80 patients with breast cancer using radioimmunoassay. the patients had been irradiated after mastectomy 7.2 +/- 1.0 years earlier with 45 gy in 3 to 4 weeks to parasternal, ipsilateral supraclavicular and axillary areas. five patients (6%) had hypothyroidism with low s-t4 and elevated s-tsh levels. in addition, 12 patients (15%) had elevated (greater than 5 mu/l) s-tsh levels and normal s-t4 values. shielding of ...	1986	3020879
nucleoside 5'-triphosphates with modified sugars as substrates for dna polymerases.	a number of nucleoside 5'-triphosphate analogs were tested with escherichia coli dna polymerase i and klenow fragment of the enzyme, bacteriophage t4 dna polymerase and calf thymus dna polymerase alpha. it was shown that 3'-amino-2',3'-dideoxynucleoside 5'-triphosphates as well as a number of 3'-derivatives of dttp(3'nh2) are able to terminate dna synthesis catalyzed by each enzyme if the reaction is performed in the absence of natural substrates. ddntp and dntp(3'f) were found to be inactive wi ...	1986	3021225
effect of calmodulin inhibitors on thyroid hormone secretion.	the effect of calmodulin inhibitors, n-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide (w-7) and trifluoperazine, on tsh-induced thyroid hormone secretion from rat thyroid was examined in vivo and in vitro. the ip administration of 5 mg w-7 to the rat inhibited t4 and t3 secretion from rat thyroids at 2, 3, and 4 h after the ip injection of 2 iu tsh, and so did the ip injection of trifluoperazine at 3 and 4 h. however, the ip injection of n-(6-aminohexyl)-1-naphthalene sulfonamide as a control ...	1986	3021438
cloning and expression of a chloramphenicol acetyltransferase gene in cytosine-substituted t4 bacteriophage.	we have developed an efficient method for transferring foreign genes into the t4 phage genome. any foreign genes inserted into the t4 uvsy gene cloned on plasmids can be transferred into a cytosine-substituted t4dc(delta nb5060) phage genome by a replacement type of recombination. to achieve this, we constructed chimeric plasmids which had a chloramphenicol acetyltransferase gene (cat) derived from transposon tn9 inserted into the bg/ii site within the t4 uvsy gene on pbr322. the cat gene was th ...	1986	3021583
t cell reactivity to penicillin: phenotypic analysis of in vitro activated cell subsets.	patients with penicillin allergy demonstrate a t cell proliferative response after in vitro stimulation with penicillin g (pen g) and other beta-lactam antibiotics. to understand better penicillin-allergic reactions, t cell subset stimulation with pen g was studied and compared with other soluble (tetanus toxoid and purified protein derivative [ppd]) and membrane-bound viral (influenza a and epstein-barr viruses) antigens. a double fluorescence method for flow cytometry was used to evaluate the ...	1986	3021832
cloning the complete riib gene of bacteriophage t4 and some observations concerning its middle promoters.	we cloned the intact t4 riib gene by joining plasmids carrying gene fragments. riib was expressed at a low level under control of the lac promoter, and the clone complemented riib mutants. we suspect that earlier attempts to clone the intact gene were unsuccessful because of transcription from t4 middle-mode promoters. these promoters are silent early in infection but are recognized when resident on a plasmid in an uninfected cell.	1986	3022011
regulation of inter- and intramolecular ligation with t4 dna ligase in the presence of polyethylene glycol.	polyethylene glycol (peg) stimulates ligation with t4 dna ligase. in 10% (w/v) peg 6,000 solutions, only intermolecular ligation is enhanced by monovalent cations, while both inter- and intramolecular ligation occur without their presence. similar stimulation was also caused by divalent cations or polyamines in the peg 6,000 solutions. such properties of the ligase could be applied to control the extent of inter- and intramolecular ligation. ligation with cations or polyamines in 10% peg 6,000 s ...	1986	3022231
nucleotide sequence of a type ii dna topoisomerase gene. bacteriophage t4 gene 39.	t4 dna topoisomerase is a type ii enzyme and is thought to be required for normal t4 dna replication t4 gene 39 codes for the largest of the three subunits of t4 dna topoisomerase. i have determined the nucleotide sequence of a region of 2568 nucleotides of t4 dna which includes gene 39. the location of the gene was established by the identification of the first fifteen amino acids in the large open reading frame in the dna sequence as those found at the amino-terminus of the purified 39-protein ...	1986	3022233
chromatin 3'-phosphatase/5'-oh kinase cannot transfer phosphate from 3' to 5' across a strand nick in dna.	rat liver chromatin contains a 3'-phosphatase/5'-oh kinase which may be involved in the repair of dna strand breaks limited by 3'-phosphate/5'-oh ends. in order to determine whether the phosphate group can be transferred directly from the 3' to the 5' position, a polynucleotide duplex was synthesized between poly (da) and oligo (dt) segments which had 3'-[32p]phosphate and 5'-oh ends. the oligo (dt) segments were separated by simple nicks as shown by the ability of t4 dna ligase to seal the nick ...	1986	3022244
herpes zoster and zosteriform herpes simplex virus infections in immunocompetent adults.	among 111 immunocompetent patients referred to a general hospital setting with the clinical diagnosis of herpes zoster, viral cultures were obtained from 47 patients. six of these patients (13 percent) had herpes simplex virus isolated, with four of the six infections involving the facial distribution, and the other two involving the t4 (breast) distribution. excluding those in whom herpes simplex virus was isolated, the mean age (+/- sd) of the remaining 105 patients was 50 +/- 19 years. thirty ...	1986	3022586
potentiation of lymphocyte responses by monoclonal antibodies to the ganglioside gd3.	previous studies have shown that the ganglioside gd3 is expressed in high concentrations on melanoma cells and that monoclonal antibodies (mabs) against gd3 may induce partial remissions in tumor growth in patients with melanoma. the present studies indicated that incubation of interleukin 2 (il2) dependent murine natural killer cells with several mabs against the ganglioside gd3 potentiated the proliferative response to il2. there was no effect on cell division in the absence of added il2. simi ...	1986	3022915
dna synthesis dependent on genetic recombination: characterization of a reaction catalyzed by purified bacteriophage t4 proteins.	to simulate a reaction that occurs in t4-infected cells, we have developed an in vitro dna synthesis system that requires seven highly purified proteins encoded by this bacteriophage: the dna polymerase "holoenzyme" (four proteins), gene 32 protein, dda dna helicase, and uvsx protein - an enzyme that catalyzes homologous dna pairing and is functionally homologous to the reca protein. in the reaction observed, the 3'oh end of one single-stranded dna molecule primes dna synthesis using a double-st ...	1986	3022939
stress-induced cruciform formation in a cloned d(catg)10 sequence.	the synthetic alternating purine-pyrimidine sequence, d(catg)10.d(catg)10, has been cloned into a 2.079-kb pbr322-derived plasmid (pln1) and its conformation studied under torsional stress. the resultant plasmid, plnc40, is hypersensitive to cleavage by the single strand-specific nucleases, s1 nuclease and bal31 nuclease, and to modification by the single strand-selective reagent, osmium tetroxide. the s1-hypersensitive site of this plasmid predominates over those previously mapped in pbr322. si ...	1986	3023073
elevation of plasma aldosterone levels of tadpoles at metamorphic climax.	plasma aldosterone concentrations in rana catesbeiana tadpoles during metamorphosis were determined by radioimmunoassay. aldosterone levels were relatively low (0.7-1.4 ng/ml) during pre- and prometamorphosis. at the onset of climax, aldosterone concentration increased markedly and reached a peak (10.9 ng/ml) at stage xxii. subsequently, aldosterone levels declined and at the end of metamorphosis (stage xxv), the average concentration was 3.9 ng/ml plasma. administration of acth elevated plasma ...	1986	3023178
plasma renin activity, active and inactive renin concentrations, and their responses to beta 1-adrenoceptor blockade with metoprolol in hyperthyroidism.	plasma renin activity (pra), plasma renin concentration (prc), inactive renin concentration (irc) and total renin concentration (trc) were measured in 31 normal controls and in 8 patients with hyperthyroidism. trc was determined as angiotensin i generated with sheep renin substrate after an acid activation of plasma. the angiotensin i of non-acidified plasma was expressed as prc. irc was calculated as trc minus prc. the mean values for pra, prc, irc and trc were significantly (p less than 0.05 t ...	1986	3023226
hypothalamic-pituitary-thyroidal dysfunctions in anorexia nervosa.	there are clinical similarities between anorexia nervosa and hypothyroidism. circulating levels of t4 and particularly t3 have been reported to be low in this eating disorder. previous reports have, however, shown normal basal levels of serum tsh with normal or delayed responses to trh. to assess thyroid function and the hypothalamic-pituitary axis in 21 women with anorexia nervosa, serum levels of free and total thyroid hormones, binding proteins, and tsh employing an extremely sensitive assay ...	1986	3114819
enzymatic synthesis and some properties of a model primitive trna.	a model primitive trna with the nucleotide sequence ggccaaaaaaaggccp was synthesized using t4 rna ligase. the nucleotide sequence of this newly synthesized oligonucleotide was confirmed by ladder analysis of several enzymatic digestion products. the secondary structure of the oligonucleotide was examined by comparison of the products of its digestion by single- and double-strand-specific nucleases with those of the digestion of the intermediate oligonucleotide ggccaaaaaaaoh. the results indicate ...	1986	3104604
effect of hypometabolism on cell injury.	rats were given galactosamine 1 g/kg i.p. pretreatment with propylthiouracil 14 days prior to intoxication and surgical thyroidectomy 10 days prior to intoxication significantly reduced the elevation of sgot and gldh activities and protected against hepatocellular necrosis 28 h after intoxication. t3 and t4 levels decreased significantly within the first 2 days in patients with acute myocardial infarction, while rt3 levels were found to be elevated within this period. trh and tsh decreased durin ...	1986	3104654
thyroxine kinetics in nonthyroidal illnesses.	free t4 estimates by equilibrium dialysis appear to reflect serum free t4 levels in vivo in patients with nonthyroidal illnesses. however, patients with either elevated or modestly reduced free t4 values appear to be clinically euthyroid and have relatively normal free t4 availability to tissues as judged by the normal production rates of reverse t3 from t4, and by the minimally altered tsh status. thus, increased or modestly reduced serum free t4 levels by equilibrium dialysis in patients with ...	1986	3104439
influence of hbv replication and delta agent superinfection on t cell subsets and killer (leu 7+) in chronic hepatitis b virus infection.	peripheral blood t-lymphocyte subsets and cells reacting for leu 7 antigen, which identifies a subset of killer and natural killer cells, have been examined in 32 patients chronically infected by the hepatitis b or d viruses (hbv, hdv) and in 28 normal subjects. the t8+ lymphocytes were increased and the t4/t8 ratio was decreased in patients with hbv replication (identified by the presence of hbcag in liver and hbv-dna in serum) and in patients with hdv infection (hdag in liver). these patients ...	1986	3104445
inherited thyroxine-binding globulin excess. study in a kindred.	a three-generation family with hereditary high thyroxine-binding globulin (tbg) serum levels has been studied. all patients were clinically euthyroid and did not have a goiter. four females of the eleven members of the kindred had elevated tbg (range: 34-56 micrograms/ml), total thyroxine (t4) and total triiodothyronine (t3) levels, but normal free t4 (ft4) and normal response of thyroid stimulating hormone (tsh) to intravenous application of thyrotropin-releasing hormone (trh). microheterogenei ...	1986	3104070
function of the lfa-1 and t4 molecules in the direct activation of resting human b lymphocytes by t lymphocytes.	activated t lymphocytes can provide all of the signals necessary to induce the proliferation of resting b lymphocytes. the activation signal is presumably initiated through direct t-b lymphocyte contact. the role of the leukocyte function antigen-1 (lfa-1) and t4 molecules in the activation of purified, small b lymphocytes by mitomycin c-treated t lymphocytes was examined by using monoclonal antibodies that react with and inhibit the function of these molecules. anti-lfa-1 antibody binding signi ...	1986	3102246
the trh test in rats with chronic chagas disease.	trh was studied in rats with chronic chagas disease. it was found an increased secretion of tsh 60 minutes after intraperitoneal injection of the hypothalamic hormone. t3 and t4 were also decreased in basal conditions. this may indicate a primary hypothyroidism.	1986	3102263
glyburide does not alter thyroid function.	goiter and hypothyroidism have been reported as side effects of sulfonylurea therapy. to test the effects of glyburide, a new generation sulfonylurea drug, on thyroid function, we studied 15 male type 2 diabetic patients before and after 6 weeks of treatment with this drug, and we repeated the studies on 9 of these patients who remained on the drug for at least 24 weeks. all hypoglycemic agents were discontinued for 1 week before the study. patients had a baseline thyroid examination, serum t4, ...	1986	3103970
aspects of thyroid function during use of norplant implants.	plasma thyroxin (t4), triiodothyronine (t3), t3-uptake and thyroid stimulating hormone (tsh) were measured in 18 women using norplant implants or norplant-2 implants for six months. free t4-index and free t3-index were also calculated. all women had used non-hormonal contraceptives before insertion of the implants. t4 decreased and t3-uptake increased, indicating a lower level of thyroid binding proteins during treatment. free t4-index remained unchanged, indicating that the free concentration o ...	1986	3103979
effect of thyroxine-binding globulin (tbg) on thyroxine (t4) uptake by human peripheral mononuclear cells: evidence of tbg-dependent uptake of t4.	the effect of sialylated tbg and desialylated tbg on thyroxine (t4) uptake by human peripheral mononuclear cells was investigated in vitro. [125i]-t4 uptake was observed when the cells were incubated with free [125i]-t4. the uptake was inhibited in a concentration dependent manner when tbg was added. during the incubation, [125i]-t4 binding to tbg was observed. [125i]-t4 incorporation into cells was also observed when the cells were incubated with [125i]-t4-sialylated tbg or with [125i]-t4-desia ...	1986	3104020
thyroid hormone response to thyrotrophin releasing hormone (trh) in the sex-linked dwarf chicken.	the effect of an injection of thyrotrophin releasing hormone (trh) on plasma levels of thyroid hormones was studied in dwarf and normal rhode island red chickens with similar genotypes other than for the sex-linked dwarf gene dw. the sex-linked dwarf chickens had different plasma iodothyronine levels from control normal chickens: high thyroxine (t4), low triiodothyronine (t3) and similar reverse t3 (rt3) levels. the injection of trh (10 micrograms/kg) in 5-day- and 5-week-old normal chickens inc ...	1986	3104021
clinical, immunological, and virological aspects in japanese hemophiliacs and aids patients.	to date, 16 cases of aids have been officially reported in japan: 8 of them were hemophiliacs and the others were male homosexuals. we had two hemophiliacs with aids, and describe these cases in this paper. one was a 48-year-old man with hemophilia b and the other was a 62-year-old man with hemophilia a. both had arc symptoms before developing overt and fatal opportunistic infections, candidiasis and a combination of atypical mycobacteriosis and aspergillosis. impairment of cellular immunity suc ...	1986	3103637
prevalence of antibodies to aids-associated retrovirus in in- and out-patients in japan.	serum specimens from 252 recipients of factor viii and/or factor ix (237 hemophiliacs and 15 nonhemophiliacs), 99 blood transfusion recipients, 269 chronic diseases, and 366 healthy subjects (included blood donors, hospital personnel and family members of hemophiliacs) were tested for reactivity to lymphadenopathy-associated virus/t-lymphotropic retrovirus type iii (lav/htlv-iii) by elisa test kit (abbott), and the presence of the antibodies to lav/htlv-iii was confirmed by indirect immunofluore ...	1986	3103640
[congenital hypothyroidism. current viewpoints and developments].	since 1978 neonatal screening on congenital hypothyroidism (cht) is performed in the netherlands. a combined t4 and tsh determination is used. this creates the possibility to detect secondary and tertiary hypothyroidism. the number of false-positive cases is relatively high. this number decreases by adjusting the method of screening. recent studies show a cht incidence of 1:2100 newborns. in about 25% of these cases the cht appears to be transient. proceeding from the screening on cht several in ...	1986	3103255
abnormalities of lymphocyte subsets and anti-lav/htlv-iii status in greek hemophiliacs.	peripheral blood lymphocyte subsets and the incidence of lav/htlv-iii antibodies were studied in 63 patients with hemophilia a who had been transfused with a low dose regimen of commercial (u.s.a.) factor viii concentrates. five patients with hemophilia b were also included in this study. in hemophilia a patients a significant reduction in the percentage and absolute numbers of t4+ cells and of the t4/t8 ratio and a significant increase in the percentage of t8+ and leu-7+ cells were observed. th ...	1986	3101712
[evaluation of the parameters used in monitoring hypothyroid patients treated with l-thyroxine].	hormonal and clinical data on 38 tests performed in 7 hypothyroid patients given substitutive treatment with l-thyroxin were analysed. the aim of the survey was to assess the dependability of the various parameters used to estimate dose adequacy in substitutive treatment. examination of individual cases revealed that overdoses of thyroxin increased t4 to above normal limits without increases in t3, ft3, ft4 or hypophyseal suppression. chronological discrepancies between clinical data and normal ...	1986	3101002
the results of a series of 963 patients with transitional cell carcinoma of the urinary bladder primarily treated by radical megavoltage x-ray therapy.	the results are reported of a large series of patients with transitional cell cancer of the bladder, treated in edinburgh between 1971 and 1982. analysis of pre-treatment characteristics for patients with transitional cell bladder cancer showed that tumour category was significantly associated with grade and tumour size. complete local tumour regression at follow-up cystoscopy was achieved in 45.9% of patients who completed radical megavoltage x-ray therapy. patients with grade 2 or 3 cancer, a ...	1986	3101140