Publications

immunofluorescent assessment of tumour infiltrating cells in laryngeal carcinoma. application of monoclonal antibodies.	in order to gain some insight into host cell accumulations within primary tumour, frozen sections from surgical specimens of laryngeal carcinoma were subjected to indirect immunofluorescence using a panel of monoclonal antibodies against various human lymphocyte subsets as well as macrophages. in addition, polyclonal antibodies against ig were used in order to trace b cells. numerous host cell infiltrates seen at the tumour periphery were composed of t4 (helper) lymphocytes and macrophages. lymp ...	1986	2946139
time relationship of immune changes to htlv-iii/lav seroconversion in patients with hemophilia.	longitudinal immune studies of patients with hemophilia a were begun in 1982 by the regional hemophilia center in st. louis, missouri. serum samples collected from 74 participants between 1982 and 1985 were analyzed for antibody to human t-lymphotropic virus type iii (htlv-iii)/lymphadenopathy-associated virus (lav). the incidence of antibody to htlv-iii/lav has increased significantly in this population of patients with hemophilia. only one of eight hemophiliacs had detectable antibody before j ...	1986	2946253
cloning of the lambda resistant genes from brevibacterium albidum and proteus vulgaris into escherichia coli.	genes from proteus vulgaris atcc13315 and brevibacterium albidum atcc15831 were introduced into escherichia coli, which rendered the host resistant to coliphage lambda. the clones transformed by any one of the two recombinant plasmids, prmg101 or prmg216, were totally resistant against the infection of virulent lambda and n4, but sensitive to ø80, t4 and t7. however, when maltose transport systems of the clones were induced by maltose, the clones were no more resistant to the phage: thus, this p ...	1986	2946296
[polymorphism of human thyroxine binding serum globulin (tbg); existence of a new form of serum protein without detectable triiodothyronine binding power].	two kinds of tbg polymorphism are described in human, one found in deglycosylated tbg from individual blood donors, the other is a genetically determined polymorphism. tbg from plasma samples from a patient with toxic goiter, not autoimmune, (p)tbg, from the patient's mother (m)tbg and from individual donors (n)tbg, were labeled with [125i]t4 or [125i]t3 and submitted to isoelectric focusing (ief), followed by autoradiography. three faint [125i]t4 radiolabeled bands were detectable in (p)tbg whi ...	1986	2946372
hypothesis: aids is an autoimmune disease directed at the immune system and triggered by a lymphotropic retrovirus.	by attaching to the cd4 (t4) molecule of the helper-inducer lymphocyte, the human immunodeficiency virus (hiv) envelope imitates the normal ligand for this receptor, namely, an invariant component of the class ii major histocompatibility antigen (mhc). depending on the degree of antigen mimicry, the normal immune response to retrovirus envelope would be expected to recognize and cross-react to self-mhc. by disguising as "self" the virus then provokes an autoimmune attack of class-ii-bearing cell ...	1986	2946499
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.	physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...	1986	2946570
rapid phosphorylation and modulation of the t4 antigen on cloned helper t cells induced by phorbol myristate acetate or antigen.	a cell surface protein known as t4 (cd4, leu3), mr = 55,000, is expressed on the subset of human t lymphocytes which provides helper function for b cell and cytotoxic t cell activities. we show that t4 is constitutively phosphorylated and that phorbol myristate acetate (pma) induces a rapid serine phosphorylation which is followed by a fast dephosphorylation. within 5 min after pma treatment, there is a 24% reduction of t4 on the cell surface, by 4 h the loss is nearly complete, and by 20 h t4 i ...	1986	2946683
the effect of nutritional support on t-lymphocyte subpopulations in protein calorie malnutrition.	t-lymphocyte subpopulations were measured in the two major types of adult malnutrition, adult marasmus and kwashiorkor-like hypoalbuminemic malnutrition. the population of t-cells (t3) and the percentage of both helper (t4) and suppressor (t8) t-cells were significantly (p less than .05) decreased in patients with kwashiorkor-like hypoalbuminemic malnutrition, but did not differ from control values in patients with adult marasmus. the ratio of helper t-cell (t4), to suppressor t-cells (t8) (rang ...	1986	2946750
defective suppressor cell function mediated by t8+ cell lines from patients with progressive multiple sclerosis.	activated suppressor cell function, induced with either concanavalin a or okt3 and mediated by either unfractionated mononuclear cells or "panning" enriched t8+ cells, freshly isolated from peripheral blood, is reduced in patients with progressive multiple sclerosis (ms) as compared with control donors. in this study, we generated t8+ cell lines from the peripheral blood of these same patients and controls. suppressor activity, mediated by t8+ cells exposed to okt3 on days 1, 7, and 14 of cultur ...	1986	2946761
the interleukin 2 secretion defect in vitro in systemic lupus erythematosus is reversible in rested cultured t cells.	interleukin 2 (il 2) secretion in response to mitogenic stimulation in vitro is strongly reduced in circulating t lymphocytes from patients with sle. it is still not clear how this abnormality relates to the b cell hyperactivity in the disease. some investigators proposed that an intrinsic t helper cell defect could lead to suppressor cell dysfunction and autoimmunity. others have found that in fact increased suppressor cell activity can cause il 2 hyposecretion. in the present study we report t ...	1986	2946762
immunoregulatory function of t8 and t4 cells from synovial fluid of patients with chronic arthritis.		1986	2950734
lymphocyte subsets in the young and aging marmoset (callithrix jacchus).	t lymphocytes were studied in healthy young and old marmosets (callithrix jacchus). data indicate that monoclonal antisera to human t lymphocyte antigens can be used to identify these cells in marmosets. total t and suppressor t cells were lower in the older group; there were no differences in helper t or in b cells. helper/suppressor ratios (t4/t8) were increased in the older group. thus, immune system alternations with aging and/or immune deficiency syndromes may be studied in this animal mode ...	1986	2948018
localization of the t4 antigen/aids virus receptor in monkey and rat brain: prominence in cortical regions.		1986	2948211
immunoregulatory function of t8 and t4 cells from synovial fluid and blood of patients with rheumatoid arthritis or other forms of chronic arthritis.	the suppressor effect of synovial fluid (sf) t8 cells and blood t8 cells on the pokeweed mitogen (pwm)-induced t4 cell-dependent immunoglobulin production of autologous blood b cells was studied in nine patients with chronic rheumatic diseases (six patients with rheumatoid arthritis (ra), one patient with juvenile ra, and two patients with other forms of chronic arthritis). the suppressor effect of sf t8 cells was of the same magnitude as that of equal numbers of blood t8 cells from patients and ...	1986	2948275
characterization of a distinct subgroup of high-risk persons with kaposi's sarcoma and good prognosis who present with normal t4 cell number and t4:t8 ratio and negative htlv-iii/lav serologic test results.	three homosexual male patients with biopsy-proved kaposi's sarcoma were classified as having the acquired immune deficiency syndrome (aids) by centers for disease control criteria when first seen in 1983 and 1984. these patients, however, differed from most patients with aids and kaposi's sarcoma in having normal cd4 cell numbers and normal cd4:cd8 ratio. furthermore, these immunologic parameters remained normal for eight to 24 months of follow-up, and the disease did not progress. results of re ...	1986	2948385
physical and functional association of the t cell receptor and the t3 molecular complex on cytotoxic t cell clones that are differentially inhibitable by anti-t3 antibodies.	to examine the hypothesis that the antigen-specific t cell receptor (tcr) can function independently from the t3 complex on cytolytic t lymphocyte (ctl) clones, the physical and functional association of the t3 molecular complex and the t cell receptor has been examined on ctl clones that are differentially susceptible to inhibition by anti-t3 antibodies. from a panel of nine dpw2-specific ctl clones derived from the same donor, two clones (8.4 and 8.8) that were the most disparate in their susc ...	1986	2948659
c-reactive protein reduces the promotion of human b-cell colony formation by autoreactive t4 cells and t-cell proliferation during the autologous mixed-lymphocyte reaction.	utilizing a dual liquid/semisolid culture system with human b cells suspended in a semisolid matrix, t4 helper cells activated in the autologous mixed-lymphocyte reaction (amlr) were much more effective than t8 suppressor cells in promoting b-cell colony formation in the presence of staph protein a. c-reactive protein (crp) inhibited the promotion of b-cell colony formation by autoactivated t cells and inhibition was observed only when crp was present during the early amlr. coculture experiments ...	1986	2948663
human helper-t-cell function does not require t4 antigen expression.	the relationship between immunoregulatory t-cell function and the expression of t-cell subset-specific differentiation antigens was examined using a phenotypically anomalous human t-cell line (tcl), termed h-1. h-1 cells were found to express t11, extremely high levels of t3, but no t4 nor t8 antigen. despite their lack of t4 antigen expression, h-1 cells could be activated by coculture with pokeweed mitogen (pwm), anti-t3 antibody, or autologous b cells to provide potent help for b-cell differe ...	1986	2948666
t8 cell suppression of antigen- and mitogen-induced t4 cell dependent immunoglobulin production.	the suppressor effect of t8 cells on antigen-induced, as compared to pokeweed mitogen-induced, t4 cell dependent immunoglobulin (ig) production by b cells of healthy subjects was studied. the antigens used were purified protein derivative of tuberculin (ppd) and tetanus toxoid (tt). the suppressor effect of t8 cells on igg, igm and iga responses in co-cultures of t4 cells and b cells was significantly stronger in the pokeweed mitogen driven system than in ppd- and tt-driven cultures under the sa ...	1986	2948744
the value of lymph node biopsy in patients with the acquired immunodeficiency syndrome (aids) and the aids-related complex (arc): a morphological and immunohistochemical study of 90 cases.	the morphological and immunohistochemical findings in lymph nodes of nine patients with the acquired immunodeficiency syndrome (aids) and 81 patients with the aids-related complex (arc) are presented. three basic histological patterns were observed: follicular hyperplasia (29 cases), mixed hyperplasia (49 cases) and lymphocyte depletion (12 cases). while the first two variants were detected in typical arc patients, lymphocyte depletion was always associated with aids. immunohistochemistry on fro ...	1986	2948898
[effect of pind-avi on various ratios of helper and suppressor t lymphocytes].	the immunomodulating effect of a new heterologous antigen (pind-avi) on helper (inducer) and suppressor t cells was investigated. primarily decreased t4 values (less than or equal to mean t4 - 2 s.d.) increased after treatment with pind (p less than 0.005, using student's t-test; n = 15). primarily increased t 8 values (greater than or equal to mean t8 + 2 s.d.; obtained for normal persons) decreased after therapy (p less than 0.001; n = 10). an increase of t4/t8 ratio (p less than 0.005 and les ...	1986	2949105
immunoregulatory defect in patients with autoimmune thrombocytopenic purpura (atp).	con a-induced suppressor cells were studied in 20 patients with autoimmune thrombocytopenic purpura and 20 normal controls. percentage of suppression was determined on autologous and allogenic lymphocytes during blast transformation with phytohemagglutinin (pha). in 10 patients and 10 controls helper (t4) and suppressor (t8) subsets were also determined, using monoclonal antibodies. we found a significant decrease in patients' suppressor cell activity both with autologous and allogeneic lymphocy ...	1986	2949403
lymphocyte subpopulations infiltrating squamous carcinomas of the head and neck: correlations with extent of tumor and prognosis.	because little is known about the mechanisms involved in local tumor-host immune reactions in squamous carcinoma of the head and neck, a study was undertaken to better characterize the types of immune cells present at the local tumor site and determine their relationship to tumor extent, systemic cellular immune parameters, and clinical outcome. in 40 untreated patients, lymphocyte subsets (ls) at the tumor-host interface were quantitated immunohistologically from serial sections of frozen tumor ...	1986	2954014
expression of c-myc in stimulated t lymphocytes of the helper/inducer phenotype producing lymphokine(s) supporting multilineage colony formation.	phytohemagglutinin (pha) renders human peripheral t lymphocytes competent to replicate their dna and divide. the stimulation of peripheral t cells of the t4 phenotype by pha, which appears to be a transcription-dependent event, leads to the production and release of lymphokines supporting proliferation and differentiation of human pluripotent stem cells (cfu-gemmt). supernatants of pha-stimulated lymphocytes of the suppressor/cytotoxic phenotype (t8) failed to demonstrate reasonable activity to ...	1986	2953163
influence of sexual preference and chronic hepatitis b virus infection on t lymphocyte subsets, natural killer activity, and suppressor cell activity.	to determine whether abnormalities of cell-mediated immunity are associated with chronic hepatitis b virus infection or with homosexuality, we studied 15 homo- and 11 heterosexual men with chronic hepatitis b virus infection, as well as 11 homo- and 16 heterosexual apparently healthy men. t lymphocyte subsets, natural killer activity, and suppressor cell activity were determined. homosexual men had significantly lower peripheral blood t4/t8 ratios resulting from a significant increase in t8-posi ...	1986	2951429
[lymphocyte subpopulations and hla-dr determinations in diseases of the inner ear and bell's palsy].	thymus lymphocytes-subpopulations (t-helper t4 and t-suppressorcells t8) were determined in inner ear diseases (e.g. sudden hearing loss, neuronitis vestibularis, morbus menière, bell's palsy) by specific monoclonal antibodies. t-cell subset ratio (t4/t8) was elevated (less than 3) in about 50% of all patients. dr-typifying was performed because of the well-known control of the immunoregulation through the class ii hla-dr antigens. there was a relative risk of 5.2 in peripheral vestibular lesion ...	1986	2950069
treatment of htlv-iii/lav-infected patients with d-penicillamine.	13 asymptomatic, htlv-iii/lav-infected male homosexuals with generalized lymphadenopathy were treated with oral d-penicillamine. all patients had depressed t4/t8 ratios and 12 had impaired t-cell function. an escalating dose schedule was employed over 2-6 weeks with doses from 0.5 to 2 g/day. generalized skin rashes developed in 4 patients which required discontinuation of therapy in one patient. two patients developed mild transient elevations of hepatocellular enzymes. reversible decreases in ...	1986	2949756
t cell subpopulations defined by monoclonal antibodies after hla-identical sibling marrow transplantation. ii. activated and functional subsets of helper-inducer and cytotoxic-suppressor subpopulations defined by two-colour fluorescence flow cytometry.	two-colour fluorescence flow cytometry was utilized to define subsets within the leu-3+ (t4+) helper-inducer and the leu-2+ (t8+) cytotoxic-suppressor t cell subpopulations in recipients of unmanipulated hla-identical sibling bone marrow transplants. the absolute number of activated cytotoxic-suppressor t cells expressing the hla-dr and okt10 activation antigens was increased. the proportion (or relative number) of cells bearing each of these activation antigens was also elevated in both the le ...	1986	2971408
modulation of myocardial l-triiodothyronine receptors in normal, hypothyroid, and hyperthyroid rats.	to characterize the nuclear receptor believed to mediate the thyroid hormones' actions on the heart, binding of l-[125i]t3 to extracts of myocardial cell nuclei from normal, propylthiouracil, and t4-treated rats has been investigated. analysis of in vitro iodothyronine binding to this solubilized nuclear preparation revealed multiple saturable, specific binding sites for t3. high affinity binding for t3 (kd = 4.2 +/- 1.0 x 10(-10) mol/l), and lower affinity (kd approximately 10(-8) mol/l) bindin ...	1986	3001476
binding of htlv-iii/lav to t4+ t cells by a complex of the 110k viral protein and the t4 molecule.	human t-lymphotropic virus type iii (htlv-iii) or lymphadenopathy-associated virus (lav) is tropic for human t cells with the helper-inducer phenotype, as defined by reactivity with monoclonal antibodies specific for the t4 molecule. treatment of t4+ t cells with monoclonal antibodies to t4 antigen blocks htlv-iii/lav binding, syncytia formation, and infectivity. thus, it has been inferred that the t4 molecule itself is a virus receptor. in the present studies, the surfaces of t4+ t cells were l ...	1986	3001934
transferrin synthesis by inducer t lymphocytes.	transferrin (tf) is a growth factor that transports iron in plasma. it is essential for proliferation of activated t lymphocytes. previous studies have suggested that peripheral blood cells are capable of synthesizing tf. using in situ hybridization techniques and human tf complementary dnas as probes, peripheral blood cells have been examined for sites of tf messenger rna (mrna) transcription. the studies described here demonstrate that tf is synthesized by a specific subset of t lymphocytes, t ...	1986	3005367
persistent infection of chimpanzees with human t-lymphotropic virus type iii/lymphadenopathy-associated virus: a potential model for acquired immunodeficiency syndrome.	the lymphadenopathy-associated virus (lav) prototype strain of human t-lymphotropic virus type iii/lav was transmitted to juvenile chimpanzees with no prior immunostimulation by (i) intravenous injection of autologous cells infected in vitro, (ii) intravenous injection of cell-free virus, and (iii) transfusion from a previously infected chimpanzee. all five animals that received more than one 50% tissue culture infective dose were persistently infected with lav or chimpanzee-passaged lav for up ...	1986	3005641
specific lysis of varicella zoster virus-infected b lymphoblasts by human t cells.	epstein-barr virus-transformed human b cells expressed cell surface varicella-zoster virus (vzv) antigens after superinfection with vzv although they did not form infectious centers in a plaque assay. the vzv-superinfected cells were lysed by autologous vzv-stimulated t-cell lines and their derivative clones. the effector cells were specific for vzv and an hla dr antigen and were t4+. the specificity of lysis of epstein-barr virus-transformed, vzv-superinfected targets by prestimulated mononucle ...	1986	3005647
regulation of thyrotropin secretion by the central epinephrine system. studies in the chronically cannulated rat.	in the present investigation cns epinephrine (epi) biosynthesis was selectively interrupted with specific norepinephrine n-methyltransferase (nmt) inhibitors, sk&f 64139 (smith, kline & french laboratories) and ly 78335 (eli lilly & co. research laboratories), to determine the effects of central epi depletion on basal and cold, thyrotropin-releasing hormone, and hypothalamic somatostatin antiserum induced thyrotropin (tsh) secretion in chronically cannulated rats. because these nmt inhibitors al ...	1986	3005895
stabilization of t4 polynucleotide kinase by macromolecular crowding.	t4 polynucleotide kinase rapidly loses activity during its reaction on duplex dna termini. addition of high concentrations of nonspecific polymers reverses or prevents this inactivation. in contrast, additions of related materials of lower molecular weight are relatively ineffective in stabilizing the kinase. such a pattern suggests that the stabilizing effects of polymers on kinase activity are due to macromolecular crowding. an effect of crowding on the known tendency of the kinase to undergo ...	1986	3005982
bacteriophage t4 dna topoisomerase mediates illegitimate recombination in vitro.	we have found that purified t4 dna topoisomerase promotes recombination between two phage lambda dna molecules in an in vitro system. in this cross, t4 dna topoisomerase alone is able to catalyze the recombination and produce a linear monomer recombinant dna that can be packaged in vitro. atp is not required for this recombination, while oxolinic acid stimulates it. the recombinant dna molecules contain duplications or deletions, and the crossovers take place between nonhomologous and nonspecifi ...	1986	3006033
replicative and cytopathic potential of htlv-iii/lav with sor gene deletions.	the genome of the human t-lymphotropic virus type iii (htlv-iii/lav) has the potential to encode at least three polypeptides in addition to those encoded by the gag, pol, and env genes. in this study, the product of the sor (short open reading frame) region, which overlaps the 3' end of the pol gene, was found to be a protein with a molecular weight of 23,000. an assay was developed for testing the ability of cloned htlv-iii proviruses to produce viruses cytopathic for t4+ lymphocytes. in the ce ...	1986	3006244
half-site editing: an in vitro mutagenesis procedure for truncating a dna fragment and introducing a new restriction site.	half-site editing is an in vitro mutagenesis procedure designed for use in making precise plasmid constructions. like many in vitro mutagenesis techniques, this procedure involves hybridization of a mutagenic oligonucleotide primer to single-stranded template dna followed by polymerization with dna polymerase i (klenow). half-site editing differs from other techniques in two main ways. first, t4 dna polymerase treatment truncates the target dna at a point determined by the primer and repairs any ...	1986	3006543
htlv-iii status and abnormalities in t lymphocyte distribution in children with hemophilia a.	children with hemophilia a are at risk for the acquired immunodeficiency syndrome (aids). clinically asymptomatic hemophiliacs demonstrate many immune abnormalities that might represent exposure to the aids agent through blood products or be a natural reaction to their therapy. in this study, we examined lymphocyte subset distribution in children with hemophilia a who had been exposed to htlv-iii as determined by antibody seroconversion. seroconversion to htlv-iii was confirmed using western blo ...	1986	3006971
endonuclease vii resolves y-junctions in branched dna in vitro.	endonuclease vii (gp 49 of phage t4) resolves four-way junctions in branched dnas. we have extended our investigations of the specificity of endo vii and tested its activity with three-way junctions (y-structures) constructed in vitro. both 'closed' and 'open' y-structures were made, absolutely identical in sequence but differing from each other by a single nick in one of the three arms. pure y-structures were obtained on a preparative scale by annealing plus and minus strands from two m13mp str ...	1986	3007114
cloning of the bacteriophage t4 uvsx gene and purification and characterization of the t4 uvsx recombination protein.	the bacteriophage t4 uvsx gene is a nonessential gene required for normal levels of dna repair, recombination, and replication. we demonstrate that plasmids containing the t4 dna approximately 300-2900 base pairs upstream of t4 gene 41 express a biologically active uvsx protein. this uvsx protein imparts increased survival to uv-irradiated t4 uvsx- phage and decreases the t4 uvsx- mutant suppression of a conditionally lethal t4 mutant in the gene 49 recombination nuclease. the uvsx protein purif ...	1986	3007520
[multiple enzymatic changes in t4 and t8 lymphocytes in primary hypogammaglobulinemia in the adult].		1986	3007878
alteration in activities of na,k, atpase, sugar transport, and insulin receptors in erythrocytes from hyperthyroid patients.	to investigate the selectivity of the reduction in na,k, atpase activity in erythrocytes from patients with hyperthyroidism, we have assessed cytochalasin b-sensitive galactose uptake and insulin receptors as well as na,k, atpase-mediated rubidium uptake in erythrocytes from 11 patients with hyperthyroidism, 5 patients after treatment and 11 normal controls. there was a significant reduction in the vmax values for rubidium uptake and the number of insulin receptors (23% and 20% below control, re ...	1986	3007924
cloning and expression of cdna encoding mouse tyrosinase.	we have isolated a pigment cell-specific cdna clone from a b16 mouse melanoma cdna library by differential hybridization. the mrna of isolated cdna is highly expressed in b16 melanoma cells and in black mouse (c57bl/6) skin, but is not detectable in mouse neuroblastoma cells nor in k1735 mouse amelanotic melanoma cells. the protein sequence deduced from the nucleotide sequence of the cloned cdna shows significant similarity to the entire region of neurospora tyrosinase. to know the identity of c ...	1986	3008090
[imaging of prostatic cancer by 1.5 tesla nuclear resonance tomography].	twenty-two patients with histologically confirmed carcinomas of the prostate were examined by nuclear magnetic resonance, using a 1.5 tesla magnet (stage t1: one patient, t2: eight patients, t3: six patients, t4: seven patients). in 19 out of the 21 patients in stages t2 to t4, the tumour showed a specific signal intensity. in 12 cases, the tumour signal was more intense than from a normal prostate when using medium repetition and echo delay times; in 19 cases, multi-echo sequences with increasi ...	1986	3008256
htlv-iii/lav viral antigens in lymph nodes of homosexual men with persistent generalized lymphadenopathy and aids.	the presence of core antigens of retrovirus htlv-iii/lav, referred to as "aids-related virus" (av), has been sought in lymph node samples of patients with persistent generalized lymphadenopathy (pgl, 28 patients), prodromal aids (1 patient) and aids with kaposi sarcoma (3 patients). in 30 patients the deposition of viral antigens, detected by monoclonal antibodies to htlv-iii and lav, could be observed within the germinal centers (gcs) primarily within the extracellular network of immune complex ...	1986	3008562
monoclonal antibody analysis of mononuclear cells in myopathies. iii: immunoelectron microscopy aspects of cell-mediated muscle fiber injury.	we have previously obtained light microscopical immunocytochemical evidence for cell-mediated muscle fiber injury and destruction in polymyositis and inclusion body myositis. to evaluate further interactions of the different cell phenotypes with each other and with the muscle fibers, the t8, t4, and leu 7 markers in 7 cases of polymyositis and in 9 cases of inclusion body myositis were localized by immunoelectron microscopy. in the early stages of the cell-mediated process, t8+ cells and macroph ...	1986	3008636
abnormalities of in vitro immunoglobulin production in apparently healthy haemophiliacs: relationship with alterations of t cell subsets and with htlv-iii seropositivity.	the pokeweed mitogen (pwm)-induced immunoglobulin (ig) production by cultures of peripheral blood mononuclear cells (pbmc) was reduced in healthy haemophiliacs treated with commercial factor viii (or ix) concentrate, whereas the spontaneous igg synthesis in vitro was enhanced. pwm-induced ig production was lower in those who had received greater amounts of concentrate, in those with inverted t4/t8 lymphocyte ratios and in those with antibody to htlv-iii. the spontaneous igg production in vitro w ...	1986	3009063
biosynthesis of a novel thyroxine-binding protein (27k protein) in human hepatoma (hep g2) cells.	human hepatoma (hep g2) cells were shown to synthesize and secrete a novel t4-binding protein, called 27k protein for its apparent mol wt on sodium dodecyl sulfatepolyacrylamide gel electrophoresis. the mrna coding for this protein was characterized by immunoprecipitation of [125i]t4 bound to 27k protein secreted into the medium of oocytes injected with total hep g2 rna. sucrose gradient fractionation of rna from hep g2 cells showed that tbg mrna and 27k mrna had different sizes, indicating that ...	1986	3009158
iodine suppression of iodide uptake in frtl-5 thyroid cells.	exposure of frtl-5 cells to iodide (i-) in excess of 3 microm suppresses the concentrative uptake of i-. the depression of i- uptake measured at the steady state is due to decrease in the rate of i- influx and not to an effect on i- efflux. exposure to nai is associated with decreased t4 secretion and also depressed na+-dependent amino acid accumulation. the depression in i- and amino acid transports increases proportionately with the duration of exposure and concentration of i- used but is not ...	1986	3009160
vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection.	eight vero cell sublines (vero t) persistently infected with wild type tacaribe virus replicated in different hosts were established. in order to unravel the mechanism involved in the initiation and maintenance of persistence, the properties of virus shed by the sublines and the presence of interfering particles (ip) were analyzed. during the course of infection, persistent virus (tac-pi) underwent mutations although no consistent pattern of virus evolution was observed. ts mutants were isolated ...	1986	3033931
epstein-barr virus (ebv) and x-linked lymphoproliferative syndrome (xlp).	xlp was first described in 1975, when ebv was still focused on as an immediate oncogenic agent, but with some uncertainties raised by the absence of ebv in most non-endemic burkitt lymphoma. the discovery of xlp refreshingly evidenced and popularized the concept, "ebv pathogenicity (oncogenicity) in immunocompromised hosts", which was later vastly substantiated by ebv-carrying lymphomas in organ-transplanted and aids patients. fatal (or severe) im, acquired hypogammaglobulinemia (ah) and maligna ...	1986	3030340
acute endocrine effects of a single administration of methylmercury chloride (mmc) in rats.	the acute effects of methylmercury chloride (mmc) on the endocrine functions were investigated with doses too small to cause any typical neurological dysfunctions. the hormones included prl, lh, tsh, acth, corticosterone (bk), testosterone (tli), total thyroxine (t4) and free thyroxine (free t4). the changes in serum hormone levels from 1 hour through 10 days after a single injection of mmc (12 mg/kg s.c.) (exp. 1), and dose-response relationships between mmc doses (2 to 16 mg/kg s.c.) and the s ...	1986	3030712
effects of stimulated adrenocortical activity on the concentration of thyroxine and triiodothyronine in blood serum of piglets.	the effects of elevated adrenocortical activity, induced by cold exposure, weaning or starvation, or exogenous adrenocorticotrophin (acth) on the concentrations of thyroxine (t4) and triiodothyronine (t3) in blood serum were investigated in 99 sucking piglets and weaners, divided into five experiments. no changes in concentrations of circulating iodothyronines were recorded during a 3-hour exposure of 3- to 4-day-old piglets to ambient temperature of 8-12 degrees c. two days after weaning of 4-w ...	1986	3030785
thyroid and pituitary hormone responses to trh in advanced nonalcoholic liver disease.	basal t4, t3, tsh, prolactin and growth hormone levels were determined in several groups: patients with postnecrotic cirrhosis with hepatocellular carcinoma (n = 14); patients with postnecrotic cirrhosis but without hepatocellular carcinoma (n = 26); cholangiolar carcinoma (n = 9); and normal controls age-matched to within 5 yr of the liver disease subjects studied. in addition, trh stimulation (400 micrograms trh) was performed; tsh, prolactin and growth hormone responses over a 180-min time in ...	1986	3033051
effects of thyroid hormones and reverse-triiodothyronine (rt3) pretreatment on beta-adrenoreceptors in the rat heart.	effects of thyroxine (t4), triiodothyronine (t3) or reverse-triiodothyronine (rt3) in vivo pretreatment or the effect of hypothyroidism on properties of beta-adrenoreceptors in the rat heart were investigated. beta-adrenergic antagonist hydroxybenzylpindolol (hyp125i) was used to estimate the maximal binding capacity (mbc) and the affinity (kd) of beta-adrenoreceptors. both t4 and t3 in dose and time dependent manner increased mbc value but only slightly and insignificantly affected affinity of ...	1986	3030048
transient phenytoin induced iga deficiency and permanent ige increase.	this is a report of a 9-year-old epileptic boy, who was studied over a period of 7 years. the seizures started when he was 2 months old. he was treated with phenytoin from the age of 2 years and 7 months. serum and salivary iga were absent with high ige serum total. the routine immunologic studies were normal. the iga was normalized after phenytoin withdrawal, but ige determination increased progressively without any atopic symptoms. the t4 (helper)/t8 (suppressor) ratio decreased (1.0 and 1.2) ...	1986	3030084
t4 polynucleotide kinase: macromolecular crowding increases the efficiency of reaction at dna termini.	the amount of reaction catalyzed by t4 polynucleotide kinase on a variety of its substrates is greatly increased in the presence of polyethylene glycol 8000 (peg 8000). both the forward and reverse reactions as well as the exchange reaction can be stimulated. the stimulation is a general effect on t4 polynucleotide kinase reactions involving high molecular weight dna substrates. the use of peg 8000 is particularly advantageous for labeling or removing terminal 5'-phosphate groups which are only ...	1986	3028204
nucleotide sequence of bacillus phage phi 29 genes 14 and 15: homology of gene 15 with other phage lysozymes.	the nucleotide sequence of bacillus phage phi 29 genes 14 (g14) and 15 (g15) have been determined and shown to encode proteins with molecular weights of 15,014 and 28,022, respectively. the g14 open reading frame (orf) was confirmed by sequencing a sus14(1241) mutant. gene product 15 (gp15) has considerable homology with salmonella phage p22 lysozyme and lesser homology with escherichia coli phage t4 lysozyme. putative translation signals are identified. in addition, the role of a previously des ...	1986	3027653
sequence and transcripts of the bacteriophage t4 dna repair gene uvsy.	we have cloned, sequenced and analyzed transcription of the phage t4 uvsy gene. this gene is transcribed from a single gp mota-dependent middle promoter to give a major transcript of approximately 930 nucleotides and a minor transcript of approximately 620 nucleotides. all in vivo and in vitro uvsy transcripts show anomalous migration in agarose gels. the uvsy transcript contains an open reading frame coding for an 137 amino acid [15.8 kilodaltons (kd)] uvsy protein and two unidentified open rea ...	1986	3026891
a plasmid expression vector that permits stabilization of both mrnas and proteins encoded by the cloned genes.	two new expression vectors have been constructed to take advantage of several useful properties of bacteriophage t4-infected escherichia coli. these plasmids, prdb8 and prdb9, contain the promoter region and start codon of t4 gene 32, a contiguous multiple cloning site (mcs), and translation and transcription termination signals. dna fragments inserted into the mcs are transcribed and translated at a high level in both uninfected and phage t4-infected cells. furthermore, the extreme stability of ...	1986	3026907
in vitro packaging into phage t4 particles and specific recircularization of phage lambda dnas.	concatemeric phage lambda imm434 dna packaged in vitro into phage t4 particles produced plaques on a selective host. moreover, lambda dna containing a pbr322 derivative flanked by the lambda attl and attr sites could be specifically recircularized by excisive lambda recombination to yield the pbr322 derivative. a host deficient in generalized recombination and containing a defective lambda c its prophage which provided int and xis proteins was the recipient for this plasmid derivative carried by ...	1986	3026930
antler cycle and thermolabile and thermostable alkaline phosphatase in white-tailed deer; circannual and circadian rhythms and variation after thyroxine, dexamethasone and acth administration.	the plasma levels of thermolabile (tlap) and thermostable (tsap) alkaline phosphatase were investigated in adult male white-tailed deer. distinct seasonal variation of tlap (with highly elevated levels in july) were observed, whereas tsap exhibits low concentration with a small increase in october. no circadian rhythm was found for tlap or tsap. a close correlation (r = 0.98) between tlap activity and antler weight was found. administration of acth or dexamethasone were ineffective in influencin ...	1986	3004090
molecular cloning and polymorphism of the human immune deficiency virus type 2.	we recently reported the isolation of a novel retrovirus, the human immune deficiency virus type 2 (hiv-2, previously named lav-2), from patients with acquired immune deficiency syndrome (aids) originating from west africa. this virus is related to hiv-1, the causative agent of the aids epidemic now spreading in central and east africa, as well as the usa and europe (see ref. 3 for review) both by its morphology and by its tropism and in vitro cytopathic effect on cd4 (t4) positive cell lines an ...	1986	3025743
immunohistochemical study on the distribution and significance of mononuclear cells in human breast carcinoma.	lymphocyte subsets in the tumor nests of breast carcinoma were immunohistochemically investigated and a quantitative analysis was added. the majority of cases showed predominance of t cell and suppressor t cell (t8). a decrease in number of lymphocyte subsets and the helper t (t4)/t8 ratio in the stroma of tumor nests correlated well with the progression of clinical stage and the presence of metastasis. this correlation could not be found in the peripheral region of the tumor nests. macrophages ...	1986	3026138
pyrimidine dimer dependent cleavage of single-stranded dna by t4 uv endonuclease.	t4 uv endonuclease cleaves double- and single-stranded dna with equal specificity for photo-pyrimidine dimers. thus, the enzyme can be used for mapping and quantifying pyrimidine dimers in single-stranded dna as well as in double-stranded dna. mapping of pyrimidine dimers shows that rates of uv-dimerization are not only affected by 5', 3' adjacent bases, but also by position within pyrimidine tracts. di-pyrimidines at 3' ends of tracts are more photoreactive than those at 5' ends.	1986	3026375
molecular recognition between oligopeptides and nucleic acids: novel imidazole-containing oligopeptides related to netropsin that exhibit altered dna sequence specificity.	oligopeptides have been synthesized that are structurally related to the antiviral antitumor antibiotic netropsin, but in which each of the pyrrole units is successively replaced by an imidazole moiety, as well as their di- and triimidazole-containing counterparts. these compounds bind to duplex dna with constants in the range (1.06-1.98) x 10(6) m-1 but not to single-stranded dna. since they bind to t4 dna, it is inferred that, like the parent antibiotic netropsin, they are also minor groove se ...	1986	3026455
clonal analysis and in situ characterization of lymphocytes infiltrating human breast carcinomas.	t lymphocytes were isolated from tumor biopsies in 13 patients with breast carcinomas. immunohistology with monoclonal antibodies confirmed the presence of mononuclear cell infiltrates composed primarily of t lymphocytes in all tumors studied. while the proportion of t lymphocytes expressing the t4 or the t8 surface marker varied from tumor to tumor as determined by morphometric analysis, t8+ cells were more numerous than t4+ cells in 8/12 breast tumors studied. relatively few t cells (less than ...	1986	3024832
long-term effect of iodized oil on serum thyroglobulin levels in endemic goitre patients.	serum thyroglobulin (tg) response to bovine tsh (btsh) was evaluated in 44 goitrous patients (grades iii and iv) living in conditions of chronic iodine (i) deficiency (iodine urinary excretion less than 40 micrograms i/g) and in 26 normal subjects. after the initial clinical evaluation and laboratory tests (btsh test, t4, t3, anti-tg and anti-microsomal antibodies) all goitrous patients received 1 ml i.m. of iodized oil (i-oil) and were followed up for 30 months. the btsh test was repeated at 6, ...	1986	3024868
immunological abnormalities in intravenous drug abusers and relationship to the prolonged generalized lymphadenopathy syndrome in italy.	the prolonged generalized lymphadenopathy syndrome (pgl) has been considered a prodromal condition to the acquired immunodeficiency syndrome (aids), but the clinical, virological and immunological characteristics of patients who will develop aids are not known. we report on the immunological profile of intravenous drug abusers with or without pgl in northeastern italy. we found a reduction of lymphocyte-absolute numbers with reversal of the t4/t8 ratio and decreased leu-11b+ cells. the response ...	1986	3024880
in vivo activated cytotoxic t cells in the thyroid infiltrate of patients with hashimoto's thyroiditis.	high proportions of t8+ cells with inverted t4/t8 ratio were found in freshly isolated thyroid lymphocytes from patients with hashimoto's thyroiditis. in addition, about one third of thyroid infiltrating cells expressed the tac antigen, whereas in patient peripheral blood (pb) or normal lymphocytes from pb or lymphoid organs the percentage of tac-positive cells was consistently lower than 10%. following negative selection with okt4 or okt8 monoclonal antibodies and complement, tac+ t cells were ...	1986	3024884
thyroxine and 3,5,3'-triiodothyronine bind to the same putative receptor in hepatic nuclei of rana catesbeiana tadpoles.	previous studies have provided conflicting evidence as to whether tadpole liver nuclei contain the same number of high affinity binding sites for t4 as they do for t3. inability to prepare stable tadpole liver nuclei may have contributed to these inconsistencies. thus, a study was carried out in which the in vivo binding of t4 and t3 by tadpole liver nuclei was reexamined with a greatly improved method for isolating liver nuclei. it was found that the maximum binding capacities of the nuclei for ...	1986	3004895
regulation of thyroxine 5'-deiodinase by thyroid hormones and activators of protein kinase c in gh4c1 cells.	the regulation of t4 5'-deiodinase activity was studied in cultured gh4c1 cells. enzyme activity was measured in cell sonicates as the release of radioiodide from [125i]t4. enzyme activity was stimulated 2- to 3-fold by hypothyroid serum and activators of protein kinase c, such as trh and phorbol esters. the hypothyroid serum effect was maximal by 3 h, whereas trh and phorbol esters required 6 h to achieve a maximal effect. the hypothyroid serum effect was gone within 4 h of returning the cells ...	1986	3004918
iodized oil treatment for endemic goiter does not induce the surge of positive serum concentrations of anti-thyroglobulin or anti-microsomal autoantibodies.	forty-three goitrous patients (grade ii and iii, who classification), living in areas of chronic iodine deficiency, were treated with an injection of iodized oil (470 mg iodine). serial measurements of serum thyroid hormone levels after the therapy revealed increasing concentrations of both hormones, with a significantly lower serum t3/t4 ratio, and progressively significantly lower serum tsh mean values. serum tg mean value, initially elevated (58 +/- 9 ng/ml), decreased after 6 months and retu ...	1986	3023472
a new membrane-associated dna replication protein, the gene 69 product of bacteriophage t4, shares a patch of homology with the escherichia coli dnaa protein.	a new phage t4 dna replication protein, gp69, is found to be associated with membrane fractions, as predicted by the translated base sequence of gene 69. in addition, gp69 shares a patch of homology with a segment of the escherichia coli dnaa initiation protein. the patchy homology of dnaa protein and gp69 suggests that they may serve some similar functions, such as interactions with the same e. coli components in bacterial and viral dna replication. we have shown before that gene 69 spans an or ...	1986	3023622
topoisomerization of plasmid dna in escherichia coli infected with bacteriophage t4.	the degradation of host dna, and the block to transcription of cytosine-containing dna, which are a part of the normal course of infection by bacteriophage t4, can be eliminated in an appropriate t4 genetic background (designated as our reference type, or r.t.), so that t4 late promoters carried on plasmid dna can function. the changes of topoisomer distribution that ensue when phage t4 r.t. infect escherichia coli carrying a plasmid containing a t4 late promoter were analyzed. the linking numbe ...	1986	3023639
[mapping the recognition sites for restrictase mlui on dna from bacteriophage t4].		1986	3023982
nucleotide sequence and analysis of the 58.3 to 65.5-kb early region of bacteriophage t4.	the complete 7.2-kb nucleotide sequence from the 58.3 to 65.5-kb early region of bacteriophage t4 has been determined by maxam and gilbert sequencing. computer analysis revealed at least 20 open reading frames (orfs) within this sequence. all major orfs are transcribed from the left strand, suggesting that they are expressed early during infection. among the orfs, we have identified the ipiii, ipii, denv and tk genes. the orfs are very tightly spaced, even overlapping in some instances, and when ...	1986	3024113
a simple and efficient enzymatic method for covalent attachment of dna to cellulose. application for hybridization-restriction analysis and for in vitro synthesis of dna probes.	single-stranded dnas (ssdnas) were covalently bound by a simple and efficient enzymatic method to a solid support matrix and used to develop several new procedures for gene analysis. the novel procedure to prepare a ssdna stably coupled to a solid support employed t4 dna ligase to link covalently oligo (dt)-cellulose and (da)-tailed dna. beginning with essentially any double stranded dna the procedure generates a ssdna linked by its 5' end to a cellulose matrix in a concentration of over 500 ng ...	1986	3024131
stimulation of thyroid adenylate cyclase activity by sera from patients with non-thyroidal illness.	we have previously shown that sera from many hypothyroid patients stimulated adenylate cyclase activity as measured by serum bioactive tsh concentrations produced by frtl-5 cell line. this tsh-stimulating activity did not correlate with serum immunoreactive tsh. igg fractions of these sera did not stimulate frtl-5 cells. the present study was, therefore, undertaken to investigate the thyroid stimulating activity of sera from patients with non-thyroidal illness. studies were performed in 36 patie ...	1986	3024435
induction of growth hormone (gh) receptors in adipocytes of hypophysectomized rats by gh.	the ability of gh to regulate its own receptor in adipocytes of hormone-substituted hypophysectomized rats was studied. male rats (130-150 g) were hypophysectomized and substituted with t4 and cortisone. a fixed dose of gh (bovine gh, human gh, or ovine gh), was administered for 5-6 days in three different ways: 1) two injections/day, 2) four injections/day, or 3) by osmotic minipumps. gh binding was measured in cell aliquots using [125i]human gh. in unsubstituted hypophysectomized animals, gh b ...	1986	3000727
thyroid hormone binding to isolated trout (salmo gairdneri) liver nuclei in vitro: binding affinity, capacity, and chemical specificity.	isolated nuclei from trout hepatocytes demonstrated in vitro high-affinity and low-capacity binding for 3,5,3'-tri-iodo-l-thyronine (t3). binding was reversible; the dissociation rate was 5.7 x 10(-3) min-1 at 15 degrees c. linear scatchard plots suggested a single class of noncooperative sites (kd = 1.4 +/- 0.10 x 10(-10) m; maximum binding capacity (mbc) = 62 +/- 10 fmol/mg dna). after correction for site degradation, site occupancy by endogenous t3, and the dissociation rate of endogenous bou ...	1986	3000865
nononcogenic deletion mutants of herpesvirus saimiri are defective for in vitro immortalization.	herpesvirus saimiri l-dna sequences between 0.0 and 4.0 map units (4.5 kilobase pairs) are required for oncogenicity; these sequences are not required for replication of the virus. to investigate the basis for the lack of oncogenicity of mutants with deletions in this region and to study the function of this region, we developed a reliable system for in vitro immortalization by herpesvirus saimiri. in contrast to peripheral blood lymphocytes from cotton-top tamarins (saguinus oedipus) and owl mo ...	1986	3003401
use of 125i-triiodothyroacetic acid to measure nuclear thyroid hormone receptor.	125i-triac was employed to measure hepatic thyroid hormone nuclear receptor (rt) in the rat. the binding properties of 125i-triac and 125i-t3 were compared in a 0.4 m kcl extract of a liver nuclear preparation. the order in which the stable compounds, triac, t3, t4 and rt3, competed for 125i-triac and 125i-t3 binding in liver nuclear extract was similar (triac greater than t3 greater than t4 greater than rt3), suggesting association of both radioligands with rt. scatchard plot analysis of specif ...	1986	3009172
speculations on sequence homologies between the fibronectin cell-attachment site, major histocompatibility antigens, and a putative aids virus polypeptide.	the core of the fibronectin cell-attachment site has been shown to be the tetrapeptide sequence arg-gly-asp-ser (rgds). this peptide as well as its inverted analogue ser-asp-gly-arg (sdgr) efficiently inhibit fibronectin-mediated cell attachment in vivo and in vitro. homology searches in protein data banks revealed the presence of the peptide sdgr in the alpha 2 domain of mhc class i antigens, and a variant of rgds, arg-phe-asp-ser (rfds), was found highly conserved in mhc class i (alpha 1 domai ...	1986	3009364
impact of tumor control on survival in carcinoma of the lung treated with irradiation.	the long-term results in tumor response, intrathoracic tumor control and survival are reported in patients with medically inoperable or unresectable non-oat cell and small cell carcinoma of the lung. in 376 patients with stages t1-3, no-2 carcinoma of the lung tumors, accessioned to a radiation therapy oncology group (rtog) randomized study to evaluate different doses of irradiation, a higher complete response rate (24%), intrathoracic tumor control (67%) and three year survival (15%) was observ ...	1986	3009368
structurally specific binding of halogenated biphenyls to thyroxine transport protein.	prealbumin is a major thyroxine binding protein in blood that has been well studied crystallographically and has also been proposed as a model for the thyroxine nuclear receptor in tissue. the high-affinity t4 binding site in prealbumin gave a linear plot on scatchard analysis. the interactions of selected polychlorinated biphenyls (pcbs) with prealbumin have been studied with use of computer graphics and predictions made regarding relative binding affinities for such structures. these modeling ...	1986	3009810
enhancive and suppressive effects of cytomegalovirus on human lymphocyte responses in vitro.	virus-specific lymphocyte proliferation in the presence of cytomegalovirus (cmv) without and with monocytes was studied in healthy persons. three categories of lymphocyte response could be distinguished: seropositive low responders, naturally high responders, and lymphocyte populations responding well to cmv antigen in the presence of added cmv-incubated autologous monocytes. this latter category could be identified by preincubating autologous monocytes with cmv. cmv-seronegative persons were no ...	1986	3009895
effect of thyrotropin on conversion of t4 to t3 in perfused rat liver.	the present study was undertaken to elucidate the direct effect of thyrotropin (tsh) on the conversion of thyroxine (t4) to 3,5,3'-triiodothyronine (t3) in the isolated perfused rat liver. the liver was perfused without recirculation with a synthetic medium containing 10 micrograms/dl t4 and the effect of constant infusion of bovine tsh (125 or 250 microu/ml) on the conversion of t4 to t3 was examined. t4 uptake in the perfused liver was not changed by the addition of tsh. the release of t3 (10. ...	1986	3010024
aids retrovirus induced cytopathology: giant cell formation and involvement of cd4 antigen.	the formation of multinucleated giant cells with progression to cell death is a characteristic manifestation of the cytopathology induced by the aids retrovirus in infected t lymphoid cells. the mechanism of giant cell formation was studied in the cd4 (t4/leu 3) positive t cell lines jm (jurkat) and vb and in variants of these lines that are negative for cell surface cd4 antigen. by means of a two-color fluorescent labeling technique, multinucleated giant cells in infected cultures were shown to ...	1986	3010463
effects of thyroid hormone replacement on beta-adrenergic responsiveness of food-deprived rats.	thyroid hormone levels and beta-adrenergic responsiveness after stimulation with the beta-adrenergic agonist isoproterenol were studied in four groups of male rats. the groups used were control rats, rats deprived of food for 72 h, rats administered 50 micrograms thyroxine (t4)/kg daily for 4 days, and rats deprived of food for 72 h and administered 50 micrograms t4/kg daily for 4 days. food deprivation significantly decreased serum t4 and triiodothyronine (t3) levels, and administration of t4 s ...	1986	3010747
[aids--a further development].	it has been recently established that aids is a virus infectious disease. htlv-i essentially makes t4 lymphocytes malignant and induces their abnormal proliferation. however, on the way, when htlv-i induces a decrease in the function of t4 lymphocytes, the symptoms of arc or aids take place on occasion. htlv-iii/lav possesses a central function to destroy t4 lymphocytes, but, when this function is blocked, it increases t4 lymphocyte proliferation. accordingly, the concept of carriers of the aids ...	1986	3010882
[molecular models of the interaction between t4 antigen and hla class ii antigens or the lav virus].	hla class ii beta chains contain in their aminoterminal polymorphic domain a highly conserved tetrapeptide (rfds) also present in protein f encoded by the lav virus. homology between this tetrapeptide and the fibronectin cell-attachment site (rgds) has suggested a role in cell adhesion processes. i propose here that such a structure, that i call adhesiotope, would allow stabilization of intermolecular contacts between molecules present at the surface of interacting cells or viruses. analysis of ...	1986	3011223
t lymphocyte regeneration after transplantation of t cell depleted allogeneic bone marrow.	the regeneration of t cell subsets was studied with double immunofluorescence marker methods in 37 patients who received hla matched t lymphocyte depleted bone marrow transplants (bmt) as part of the treatment for their haematological disease. a cocktail of anti-pan-t (cd6: mbg6) and anti-suppressor/cytotoxic-t cell (cd8: rft8) monoclonal antibodies was used with rabbit serum as a source of cytolytic complement to achieve selective t cell lysis. the t8+ cells reached low normal values around 60 ...	1986	3011326
structure of rna polymerase ii promoters. conformational alterations and template properties of circularized saccharomyces cerevisiae gal1-gal10 divergent promoters.	a dna fragment encompassing the saccharomyces cerevisiae gal1--gal10 divergent promoters (914 bp) has been circularized in vitro with t4 dna ligase. we have defined a set of conditions that allows the production of a series of nine topoisomers covering a range from relaxed to highly negatively supercoiled dna. topoisomers were recovered in pure form from agarose gels and were analysed singly for the presence of sites sensitive to the single strand-specific endonuclease pl. in this way, the occur ...	1986	3011425
morphine induced thyroxine release from rat thyroid gland in vitro.	male rat thyroid glands were incubated for two hours in krebs-ringer bicarbonate buffer with different amounts of morphine and/or naloxone. five micrograms/ml morphine produced a significant increase in the t4 concentration of incubation medium, and resulted in an accumulation of camp in the tissue. naloxone did not change the t4 release but its incubation with morphine prevented the morphine-induced changes. similarly, naloxone inhibited the morphine-induced accumulation of camp in the thyroid ...	1986	3011626
thyroxine therapy in patients with severe nonthyroidal illnesses and low serum thyroxine concentration.	a randomized prospective study was done to assess the response of hypothyroxinemic patients with severe nonthyroidal illnesses to t4 therapy. patients admitted to a medical intensive care unit who had a total serum t4 concentration less than 5 micrograms/dl were randomly assigned to a control (12 patients) or a t4 treatment group (11 patients). l-t4 in a dose of 1.5 micrograms/kg was given iv each day for 2 weeks. in the treatment group, serum t4 and free t4 concentrations significantly increase ...	1986	3011834
in vitro infection of human monocytes with human t lymphotropic virus type iii/lymphadenopathy-associated virus (htlv-iii/lav).	we explored the possibility that normal human monocytes can be infected with the retrovirus human t lymphotropic virus type iii/lymphadenopathy-associated virus (htlv-iii/lav). the t4 antigen, believed to be the receptor for htlv-iii/lav binding to cd4 cells, is found on monocytes at low levels. anti-t4a, which recognizes an epitope on the t4 molecule, inhibits viral binding to monocytes, and virus inhibits anti-t4a binding, although inhibition in both cases is not total. virus particles were de ...	1986	3011909
illegitimate recombination mediated by t4 dna topoisomerase in vitro. recombinants between phage and plasmid dna molecules.	illegitimate recombination dependent on t4 dna topoisomerase in a cell-free system has recently been described. in that work, recombinants between two phage lambda dna molecules were produced by the topoisomerase alone, without an escherichia coli extract. in this paper, it is shown that recombination between phage lambda and circular plasmid dna molecules can also be detected in the presence or absence of an e. coli extract but at frequencies two or three orders of magnitude lower than that obs ...	1986	3012275
functional role of hla class i cell-surface molecules in human t-lymphocyte activation and proliferation.	this investigation addressed the role of major histocompatibility complex-encoded class i molecules in the activation and proliferation of human lymphocytes. we studied the effect of antibodies specific for hla-a and hla-b locus gene products on mitogen-stimulated peripheral blood mononuclear cell (pbmc) subpopulations. three individually derived, well-characterized anti-hla class i monoclonal antibodies were demonstrated to inhibit the proliferation of human pbmc stimulated by either okt3 or th ...	1986	3012568