Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| thyroid hormone increases rat atrial natriuretic peptide messenger ribonucleic acid accumulation in vivo and in vitro. | thyroid hormone has a number of effects on cardiovascular and renal function which are shared by the atrial natriuretic peptide (anp). we attempted to demonstrate a relationship between the two by studying the effects of thyroid hormone on the expression of the anp gene and the secretion of its encoded protein. thyroid hormone, when given to thyroidectomized rats, increased plasma anp levels by approximately 2-fold in both watered and dehydrated animals. cardiac anp mrna in dehydrated animals fe ... | 1987 | 2969452 |
| studies of lymphocyte subpopulations and immunoglobulin production in iga nephropathy. | this work was undertaken to examine the immunoregulation of t lymphocytes in patients with iga nephropathy. fifty patients and thirty-seven control subjects were studied in an infection-free interval. t lymphocyte subpopulations were determined using okt monoclonal antibodies against helper (okt4) and suppressor (okt8) t cell subsets. the proportions of t lymphocyte subpopulations did not differ between patients and controls. patients with significant renal impairment demonstrated a reduced okt4 ... | 1987 | 2964968 |
| cell-populations involved in the humoral leukocyte adherence inhibition reaction. the t8-suppressor/cytotoxic cells are both effectors and responders. | in an attempt to identify the cell subpopulation involved in the humoral leukocyte adherence inhibition (h-lai) reaction, mononuclear leukocytes were fractionated and the response with the different cell populations was measured in the h-lai assay using sera from lung cancer patients and a lung cancer associated antigen. the monocytes were found to exhibit a suppressive effect on the h-lai response. the b-lymphocytes seemed not to take an active part in the reaction. depletion of the t-lymphocyt ... | 1987 | 2965016 |
| changes in the helper and suppressor lymphocytes in human peripheral blood following maximal physical exercise. | lymphocyte subpopulations and con a activated suppressors were measured before, immediately after maximal physical effort on bicycle ergometer and after two-hour recovery. the investigations were performed on healthy male volunteers, trained male and female cyclist, nearly at the same age. the measured parameters of maximal effort were comparable in all investigated groups. the significant increase in t8+ (suppressor cytotoxic) cells and moderate increase in t4+ (helper inducer) cells was observ ... | 1987 | 2965563 |
| in vitro anti-hbs antibody synthesis from anti-hepatitis b vaccine recipients. | peripheral blood mononuclear cells (pbmc) from 'responders' recently boosted with hepatitis b vaccine, were studied for synthesis in vitro of antibody to hepatitis b surface antigen (anti-hbs ab) when stimulated with pokeweed mitogen (pwm) or hbsag. hbsag alone can induce an antigen-specific anti-hbs ab response in vitro; this antibody synthesis is t cell-dependent. in some responders both allogeneic t4+ cells (in absence of pwm or hbsag) and mixed leucocyte culture supernatants (mlc/sn) (withou ... | 1987 | 2962789 |
| multiple sclerosis: ii. effects of prothymosin alpha on the autologous and allogeneic mlr in patients with multiple sclerosis. | we have recently demonstrated that peripheral blood monocytes from patients with multiple sclerosis (ms) have a defect in stimulating autologous and allogeneic t lymphocytes. this defect was found to correlate with disease activity. in this report we demonstrate that prothymosin alpha (prot alpha), a rat thymus fraction 5 polypeptide, restores the ms monocyte stimulatory defect. the concentrations of prot alpha which induced optimal enhancement of the mixed lymphocyte responses (mlr) were signif ... | 1987 | 2962790 |
| [role of ultrasensitive tsh assay and ultrarapid total thyroxine assay in the thyroid studies]. | the diagnostic value of high sensitivity thyrotrophin assay (hs-tsh) and that of ultra-short determination of total thyroxine (t4) by fluorescence polarization (t4-tdx) were evaluated in 284 patients (29 hyperthyroid, 137 euthyroid without treatment and 118 under substitutive therapy). after comparing the clinician's first impression with the results of these laboratory tests the proportion of correctly classified untreated euthyroid patients was the same with t4-tdx as with hs-tsh (90%). in con ... | 1987 | 2962120 |
| activation of resting human b cells by helper t-cell clone supernatant: characterization of a human b-cell-activating factor. | the effects of helper t-cell clone supernatants on resting human b cells were investigated. four different helper t-cell clones (two t4+ and two t8+) were stimulated by anti-t3 monoclonal antibodies on sepharose beads or anti-t11(2) plus anti-t11(3) monoclonal antibodies. the supernatants from these activated clones induced the proliferation of highly purified resting b lymphocytes from the peripheral blood. the b cells exhibited a cell size and a surface-antigen pattern (4f2 antigen and transfe ... | 1987 | 2962196 |
| familial splenomegaly syndrome with reduced circulating t helper cells and splenic germinal centre hypoplasia. | familial splenomegaly is a rare occurrence and is occasionally associated with immune abnormalities. we report three members of a family with massive splenomegaly associated with a reduction in circulating t helper cells, a reversed t4/t8 ratio and cutaneous anergy. the spleen and lymph nodes were shown in one family member to have germinal centre hypoplasia with t helper cells being present in normal numbers and distribution in these tissues. various abnormalities of immunoglobulins were also n ... | 1987 | 2962629 |
| development of aids, hiv seroconversion, and potential co-factors for t4 cell loss in a cohort of intravenous drug users. | a cohort of 334 intravenous (iv) drug users from new york city drug treatment programs were followed over a mean 9-month period. among the 165 who were seropositive at enlistment, four developed clinical aids, for an annual rate of 3%. elevated iga was a significant predictor of developing aids. among 72 subjects who were initially seronegative and who were re-interviewed, four were seropositive at follow-up, for a seroconversion rate of 7% per year among seronegatives. among seropositive subjec ... | 1987 | 2896511 |
| alterations in helper-specific circulating t lymphocytes and in the autologous mixed lymphocyte reaction in chronic hepatitis b. | defects in t lymphocyte subpopulations and in the functional characteristics of such cells have been thought to play a role in the evolution of chronic hepatitis b, but the precise nature of the alterations and their significance remains unresolved. we studied lymphocyte subsets in 27 patients with chronic hepatitis b utilizing standard monoclonal antibodies including leu 1,2a,3a,7 and d1/12 (against the common determinant of the dr molecule), as well as a newly described monoclonal antibody, "5 ... | 1987 | 2949600 |
| suppressor cell activity in melanoma-draining lymph nodes. | we previously demonstrated that the cells of lymph nodes near to a melanoma respond well to stimulation by mitogens, alloantigens, and interleukin 2 than do nodes further away. in this study we examined suppressor t-cell activity in nodes at different distances from primary melanoma, using a concanavalin a (con a) suppressor cell assay. tumor-free regional nodes were classified as proximal, intermediate, and distal relative to primary melanoma. lymph node lymphocytes (lnl) were stimulated with 5 ... | 1987 | 2949828 |
| induction of suppression following autologous mixed lymphocyte reaction; role of a novel 2h4 antigen. | in this study, we have investigated the cellular and molecular basis for immunoregulatory function of t4+ cells after autologous mixed lymphocyte reaction (amlr) activation. we demonstrated that the t4+ 2h4+ subset but not the t4+2h4- subset can proliferate maximally in response to autologous non-t cells. t4+ cells activated by amlr exerted suppressor activity on pokeweed mitogen-driven igg synthesis of autologous peripheral blood lymphocytes. the suppressor activity by amlr-activated t4+ cells ... | 1987 | 2949986 |
| subset specificity of antilymhocyte antibodies in systemic lupus erythematosus. ii. preferential reactivity with t4 + cells is associated with relative depletion of autologous t4 + cells. | using indirect immunofluorescence and flow cytometry, we determined the proportion and number of t3+, t4+, and t8+ cells in the peripheral blood of patients with systemic lupus erythematosus whose sera were positive for cold-reactive antilymphocyte antibodies versus values in patients whose sera were negative for these antibodies. there was a disproportionate reduction in t4+ peripheral lymphocytes when cold-reactive antilymphocyte antibodies preferentially cytotoxic for this subpopulation were ... | 1987 | 2950862 |
| localization of motoneurons innervating individual abdominal muscles of the cat. | the motor pools of the individual abdominal muscles of the cat were localized in studies by using either intramuscular injections of horseradish peroxidase (hrp) to retrogradely label abdominal motoneurons or electrical microstimulation of the ventral horn at different segmental levels to produce localized twitches of the abdominal muscles. the segmental distribution of each motor pool was as follows: rectus abdominis, t4-l3; external oblique, t6-l3; transverse abdominis, t9-l3; and internal obl ... | 1987 | 2951401 |
| defect in b cell function in htlv iii/lav positive hemophilia patients. | the capacity of the peripheral blood lymphocytes (pbl) to generate an antibody response in vitro t cell-dependent antigen ovalbumin was studied in 12 severe hemophilia patients who were otherwise in good health. pbl from four of 12 patients were not capable of generating such a response after stimulation in vitro, whereas all controls were normal. this negative plaque-forming cell (pfc) response coincided with the presence of antibodies directed toward human t-lymphotropic virus iii/lymphadenopa ... | 1987 | 2952185 |
| different growth rates of swarm chondrosarcoma in lewis and wistar rats correlate with different thyroid hormone levels. | the transplantable swarm rat chondrosarcoma grew to twice the weight in 5 weeks in lewis strain rats (approximately 80 g) as it did in wistar strain rats (approximately 40 g). wistar tumor passaged into lewis rats adopted the accelerated growth rate of the lewis tumor on the second passage. conversely lewis tumor passaged into wistar rats grew like wistar lineage chondrosarcoma after two passages. lewis and wistar tumors had a similar histological appearance. the extracellular matrix composition ... | 1987 | 2952454 |
| predicted structure of tail-fiber proteins of t-even type phages. | the sequences of the tail fiber protein 36 of the phages t4, t2, k3, and ox2 were analyzed for homologies and for folding patterns using structure prediction methods. no repeating motif was found. a model for the fiber structure is proposed in which beta-strands of about 6 amino acids are separated by turns. in the beta-strand, hydrophobic amino acids are found alternating with hydrophilic ones. such amphipathic beta-strands can be stabilized by dimer formation. the dimerization occurs in a para ... | 1987 | 2952522 |
| lymphocyte subpopulations in rheumatic heart disease. | monoclonal antibodies and indirect immunofluorescence techniques were used to compare the distribution of lymphocyte subpopulations of tonsil and peripheral blood from patients with rheumatic heart disease and age and socioeconomically matched patients undergoing tonsillectomy for chronic recurrent tonsillitis, but who had no evidence of rheumatic fever or rheumatic heart disease. the proportions of b cells (ba-1+), total t cells (lyt-3), inducer/helper t cells (t4+) and cytotoxic/suppressor t c ... | 1987 | 2952796 |
| neurosarcoidosis: cerebrospinal fluid lymphocyte subpopulations. | csf lymphocyte subpopulations of eight patients with neurosarcoidosis were examined. csf or ct was abnormal in all. the csf t4/t8 (helper/suppressor) ratio was elevated at 6.8 and 7.6 in two patients; in one, there were only csf t4 cells. the ratio was normal in five patients. | 1987 | 2952900 |
| the 2h4 molecule but not the t3-receptor complex is involved in suppressor inducer signals in the amlr system. | it is suggested that autologous mixed lymphocyte reaction (amlr) may play an important role in generating suppressor inducer signals and in down-regulating the immune response following self-major histocompatibility recognition. in the present study, monoclonal antibodies directed at cell surface structures on t4+ cells activated in amlr were used to define the molecules important in the generation of the suppressor inducer signal. the density of a 200/220-kda structure, termed 2h4, increased on ... | 1987 | 2953440 |
| functional studies of t4+ lymphocyte subsets distinguished by the monoclonal antibody 5/9: suppressor-effector t4+ lymphocytes derive from the 5/9+ subset. | human t lymphocytes bearing the cell surface antigen t4 are functionally heterogeneous, exerting helper/inducer, suppressor-inducer, suppressor-effector, and cytotoxic activities. other cell surface antigens with a more restricted expression may help separate t4+ lymphocytes into functionally distinct subsets. this report describes the regulatory functions of t4+ lymphocytes fractionated by the monoclonal antibody 5/9, which detects a cell surface antigen present on 50-60% of t4+ lymphocytes. th ... | 1987 | 2953445 |
| [transmission of amber mutants of bacteriophage t4. iii. thermostability of the replication of amber mutants in cells of a non-permissive host is typical for the majority of phage tail genes]. | the article deals with determination of the spreading of the earlier discovered phenomenon of the temperature sensitivity of multiplication of t4 phage amber mutants. on the basis of the study of the dependence of multiplication of 50 amber mutants in 22 genes of t4 phage tail in the cells of non-permissive host on the incubation temperature in the range of 15-41 degrees c, the following conclusion is drawn: temperature sensitivity of multiplication of amber mutants appears to be gene-specific a ... | 1987 | 2953651 |
| identification and cloning of a prethymic precursor t lymphocyte from a population of common acute lymphoblastic leukemia antigen (calla)-positive fetal bone marrow cells. | we have cloned common acute lymphoblastic leukemia (calla)-positive cells from human fetal bone marrow containing less than 1 in 10,000 e-rfc in round-bottomed microtiter wells (one cell per well) using the autocloning unit of an epics-v cell sorter. expansion of such cells (with il-2 and heavily irradiated autologous thymocytes as feeder cells) resulted in growth in 6-14% of the wells (mean, 11%) with cells with mature t lymphocyte phenotype. two-color fluorescence analysis of outgrowing cultur ... | 1987 | 2953847 |
| effects of escherichia coli ssb protein on the single-stranded dna-dependent atpase activity of escherichia coli reca protein. evidence that ssb protein facilitates the binding of reca protein to regions of secondary structure within single-stranded dna. | the effect that escherichia coli single-stranded dna binding (ssb) protein has on the single-stranded dna-dependent atpase activity of reca protein is shown to depend upon a number of variables such as order of addition, magnesium concentration, temperature and the type of single-stranded dna substrate used. when ssb protein is added to the dna solution prior to the addition of reca protein, a significant inhibition of atpase activity is observed. also, when ssb protein is added after the format ... | 1987 | 2953903 |
| atrial natriuretic peptide in hypothyroidism. | the effect of hypothyroidism on circulating levels of atrial natriuretic peptide (anp) was studied in 11 hypothyroid patients (aged 11-65 yr; mean, 31 yr) and 13 normal subjects (aged 28-39 yr; mean, 32 yr). plasma anp was 32 +/- 9 (+/- sd) pg/ml in normal subjects and 20 +/- 5 pg/ml in the hypothyroid patients (p less than 0.005). in 7 hypothyroid patients, plasma anp levels were measured after 10-14 weeks of l-t4 therapy. anp increased from 22 +/- 5 to 46 +/- 18 pg/ml (p less than 0.02), along ... | 1987 | 2947909 |
| a defect of immunoregulatory t cell subsets in systemic lupus erythematosus patients demonstrated with anti-2h4 antibody. | the cell surface phenotype of peripheral blood lymphocytes (pbl) of systemic lupus erythematosus (sle) patients was characterized with the anti-2h4 monoclonal antibody that defines the human suppressor inducer subset. the t4+2h4+ population of cells has been shown to be critical for the activation of t8+ suppressor cells. patients with sle has a markedly decreased percentage of t4+2h4+ cells (13 +/- 2%) in their pbl compared with normal controls (21 +/- 1%) (p less than 0.001). this reduction wa ... | 1987 | 2950134 |
| monoclonal antibodies reactive with swine lymphocytes. ii. detection of an antigen on resting t cells down-regulated after activation. | the expression of an antigen on porcine t lymphocytes detected by murine monoclonal antibody (mab) 8/1 was investigated by functional studies and dual-parameter immunofluorescence. mab 8/1 reacts with greater than 95% of thymocytes and in peripheral blood with all t lymphocytes and with cells of the monocyte/macrophage lineage, but not with b cells, erythrocytes, and platelets. pretreatment of peripheral blood lymphocytes with mab 8/1 plus complement abrogated the proliferative response in vitro ... | 1987 | 2950172 |
| controlling mechanisms for expression of the bacteriophage t4 beta-glucosyltransferase gene. | the plasmid pthf3047 carries a 2200 bp fragment containing the phage t4 beta-glucosyltransferase (beta gt) gene and part of the upstream gene 42 cloned in pbr313 under the control of the amp promoter p1. in t4-infected cells the beta gt gene may be expressed by a mechanism antagonizing rho action. the plasmid pthf3047 expressed about threefold higher beta-glucosyltransferase activity in a strain carrying the polarity-suppressing rho-102 mutation than in an otherwise isogenic rho+ strain, and pro ... | 1987 | 2950203 |
| visualization of cytokine and virus receptors common to the immune and central nervous system. | interleukin 1 (il-1) and the human retrovirus htlv-iii/lav represent two distinct molecular and biological constituents that are apparently shared by both the immune and central nervous systems. il-1 is a cytokine produced principally by monocytes of the immune system and glial cells of brain in situ or in vitro. htlv-iii/lav represents a unique class of human retrovirus which is the recognized etiological vector of acquired immunodeficiency syndrome (aids) which exhibits cellular tropism to hel ... | 1987 | 2950283 |
| stimulation of protein-directed strand exchange by a dna helicase. | the protein-mediated exchange of strands between a dna double helix and a homologous dna single strand involves both synapsis and branch migration, which are two important aspects of any general recombination reaction. purified dna-dependent atpases from escherichia coli (reca protein), ustilago (rec 1 protein) and phage t4 (uvsx protein) have been shown to drive both synapsis and branch migration in vitro. the t4 gene 32 protein is a helix-destabilizing protein that greatly stimulates uvsx-prot ... | 1987 | 2950327 |
| [hormonal, beta-endorphin and renin activity changes in man during partial immersion, as a therapeutic method, in water at 38 degrees c]. | this research was carried out to define the effects on men of head-out water immersion in a bath at 38.41 +/- 0.04 degrees c (mean +/- s.e.) with a method similar to that used for therapeutical rehabilitation and time of immersion of 30 minutes. beta-endorphin, renin activity, aldosterone, cortisol, hgh, fsh, lh, tsh, t3, t4 and prolactin haematic levels were analysed. seventeen healthy subjects (fourteen males and three females), aged 21-65 years (mean age 29.8 +/- 2.6) were studied. water imme ... | 1987 | 2950339 |
| [current status of the treatment of laryngeal cancer]. | the state of the art in laryngeal cancer treatment in the rsfsr is outlined on the basis of an analysis of morbidity and other statistical data. measures which can be taken to considerably improve the effectiveness of treatment are discussed. the importance of a complex of diagnostic procedures to be employed in therapy planning is shown. preoperative hbo-mediated gamma therapy and mean fractionation of radiation were used to improve combined treatment. the following function-saving procedures w ... | 1987 | 2950661 |
| [pan t, t4 and t8-positive lymphocytes in the peripheral blood of patients with systemic lupus erythematosus]. | in a group of 23 patients with systemic lupus erythematosus by means of monoclonal antibodies differentiation markers were investigated on peripheral blood t-lymphocytes. individual specific differences in the percentage of t1-, t3-, t4- and t8-positive cells were found, indicating various possibilities of immunological disorders in this disease. furthermore, in comparison with healthy donors, an increased number of t4-, t8-double stained cells was detected in the peripheral blood of patients wi ... | 1987 | 2954308 |
| effect of amiodarone on rat heart myosin isoenzymes. | the effects of amiodarone on heart weight, production of 14c-co2 from labelled glucose, myosin atpase activity, and myosin isoenzyme patterns were determined by comparing control and amiodarone-treated male wistar rats. since it has been suggested that amiodarone may interfere with thyroid hormone action on the heart, similar experiments were also carried out in hypothyroid and amiodarone-plus-triiodothyronine(t3)-treated rats, and the data were compared to those obtained in amiodarone-treated r ... | 1987 | 2954716 |
| abnormal lymphocyte function in scleroderma: a study on identical twins. | this paper describes immunologic studies on a set of identical twins discordant for the presence of scleroderma. the affected twin had a low absolute t-cell count, low numbers of t4 helper/inducer cells, and an increase in the t8 suppressor/cytotoxic cell count. the t cells of the patient responded poorly to mitogens and to allogeneic and autologous stimuli. by contrast, t-cell-helper activity for pokeweed mitogen-induced igm synthesis was markedly enhanced in the patient. furthermore, activated ... | 1987 | 2954728 |
| expression of fc epsilon receptors on activated human t lymphocytes. | our results clearly demonstrate that the low-affinity receptor for ige (fc epsilon r) is an activation antigen transiently expressed on a subpopulation of human t lymphocytes. it can be selectively induced by stimulation with certain antigens or lectins, but it is not found on resting t cells. the increased numbers of activated fc epsilon r+ t cells observed after stimulation of peripheral blood mononuclear cells (pbmc) from bee venom allergic patients with the specific allergen phospholipase a2 ... | 1987 | 2954825 |
| stimulation and inhibition of human granulopoiesis in vitro by normal and malignant t4- or t8-lymphocyte subpopulations. | we studied the role of total peripheral blood t-lymphocytes and separated subpopulations of okt4+ and okt8+ cells stimulated with concanavalin a in the regulation of human granulopoiesis. unfractionated total t cells depleted of monocytes are capable of producing colony-stimulating activity (csa) and colony-forming unit-clone (cfu-c) suppressor activity simultaneously. after positive selection of cell subsets using a fluorescence-activated cell sorter, these two activities were produced by okt4+ ... | 1987 | 2954841 |
| immunoregulatory effects of fraction 5 thymus peptides. i. thymosin alpha 1 enhances while thymosin beta 4 suppresses the human autologous and allogeneic mixed lymphocyte reaction. | thymosin alpha 1 and thymosin beta 4, two peptides isolated from preparations of calf thymus fraction 5, were tested in the human mixed lymphocyte reaction (mlr). thymosin alpha 1 was found capable of enhancing both the allogeneic and autologous mlr. on the contrary, thymosin beta 4 suppressed mlr proliferative responses. study of the responses of the t cell subpopulations revealed that t4+ (helper/inducer) cells but not t8+ (suppressor/cytotoxic) are responsible for the enhanced, proliferative ... | 1987 | 2954929 |
| the t4 molecule differentially regulating the activation of subpopulations of t4+ cells. | it has been demonstrated that the t4+2h4+ subset functioned as a suppressor inducer cell, whereas the reciprocal t4+2h4- subset provided help for b cell ig production. in the present studies, a series of monoclonal antibodies to cell surface structures expressed on these subsets of cells were examined for their effects on the proliferative and immunoregulatory functions generated in amlr. we demonstrated that anti-t4 antibody preferentially inhibited the proliferative response of the t4+2h4+ but ... | 1987 | 2955047 |
| circatrigintan hormonal and skin surface temperature rhythms following hysterectomy. | starting 2.5 weeks after removal of her uterus, but not of her ovaries, a 34-year-old, clinically healthy woman contributed a daily blood sample at 0900 and measured her skin surface temperature on her right breast above the nipple and just below the right breast daily for the ensuing 2 months. in aliquots of serum stored frozen, luteinizing hormone (lh) and follicle-stimulating hormone (fsh) were determined in the united states; these hormones and prolactin, estradiol (e2), progesterone, testos ... | 1987 | 2955424 |
| lymphocyte transformation induced by autologous cells: xviii. impaired autologous mixed lymphocyte reaction in subjects with aids-related complex. | t cells from patients with aids-related-complex (arc) and normal controls were studied for their proliferative response in the autologous mixed lymphocyte reaction (amlr). cells from arc subjects failed to proliferate in autologous mlr compared to normal controls. this defect was not attributable to an altered ratio of t4 to t8 cells, as purified t4 cells from arc subjects also failed to proliferate in autologous mixed lymphocyte culture. in contrast, t cells from arc subjects proliferated norma ... | 1987 | 2955966 |
| antigen presentation by a subset of t8+ ia+ cells to t4+ helper cells. | the helper function of human t4+ cells acting on autologous peripheral blood b cells was elicited by the hapten-carrier conjugate dnp-streptococcal antigen (dnp-sa), in the presence of monocyte-enriched cells. the antigen presenting function of monocyte-enriched cells can be replaced by a subset of autologous t8+ vicia villosa lectin adherent (t8vv+) cells. cell depletion studies confirmed that the t8vv+ cell presentation was mediated by a t cell since killing with anti-t3 antibody and complemen ... | 1987 | 2955972 |
| studies on human blood lymphocytes with ic3b (type 3) complement receptors: iii. abnormalities in patients with active systemic lupus erythematosus. | lymphocytes displaying ic3b (type 3) complement receptors (cr3) were quantified by flow cytometry in patients with systemic lupus erythematosus. the percentages and absolute numbers were compared to age and sex matched controls. total cr3+ lymphocytes identified by the monoclonal antibodies okm1 or leu 15 were significantly decreased in patients with symptomatic arthritis, serositis or vasculitis and those with lupus nephritis, whereas values for cr3+ lymphocytes in patients with inactive diseas ... | 1987 | 2955974 |
| activities of glycolytic enzymes in some brain areas of thyroidectomized rats and their response to replacement therapy. | glycolytic metabolism has been assessed by studying a set of key enzymes, in anterior cortex, amygdala, hypothalamus septum and hippocampus, in thyroidectomized rats. the reversibility of the changes induced by the thyroidectomy has been assessed by replacement therapy. in thyroidectomized rats the hexokinase activity was significantly decreased in anterior cortex and hypothalamus. the increase in phosphofructokinase and pyruvate kinase activity was probably due to an increase in cellular energy ... | 1987 | 2956088 |
| organization of double-stranded dna in bacteriophages: a study by cryo-electron microscopy of vitrified samples. | in this paper it is shown that conformation and packing of double-stranded dna within the head of bacteriophages lambda and t4 can be assessed by cryo-electron microscopy of vitrified specimens. electron diffraction patterns show that dna within vitrified bacteriophages has a b conformation. electron micrographs of vitrified bacteriophages show domains within the head formed by a approximately 2.5-nm striation and arising from the dna packing. the number of differently oriented domains seen with ... | 1987 | 2956092 |
| lymphocyte subpopulation profiles and mitogen kinetics in immunological deficiency testing. | the correlation between lymphocyte proliferative responses to mitogens and t4/t8 ratios was analyzed in a cross section of patients who either were in a high-risk group for htlv-iii infection or fulfilled clinical criteria for acquired immune deficiency syndrome (aids). the patient results showed that, correlated with decreased t4/t8 ratios, there was a decrease in mitogen responsiveness first to pokeweed mitogen (pwm), followed by concanavalin a (con a) and then phytohemagglutinin (pha). parall ... | 1987 | 2956275 |
| responses of peripheral blood cells and lymphocyte subpopulations to insulin-induced hypoglycaemia in human insulin-dependent (type 1) diabetes. | changes in counts of peripheral blood cells including lymphocytes and lymphocyte subpopulations were examined in response to acute insulin-induced hypoglycaemia in fourteen insulin-dependent diabetic patients of differing duration, and in eight normal subjects. in patients with a short duration of diabetes (less than 5 years) an initial lymphocytosis preceded a later granulocytosis, similar to changes in the normal group following hypoglycaemia. the lymphocytosis comprised increments in t11 (tot ... | 1987 | 2957208 |
| regulation by thyroid hormones of terminal differentiation in the skeletal dorsal muscle. ii. urodelan amphibians. | in the urodelan amphibian pleurodeles waltlii, spontaneous anatomical metamorphosis was correlated with an increase in the serum level of thyroxine (t4). it was also accompanied by a change in the myofibrillar atpase profile of the dorsal skeletal muscle; fibers of larval type were gradually replaced by the adult fiber types i, ii a, and ii b. likewise, a myosin isoenzymic transition was observed in dorsal muscle, larval isomyosins were replaced by adult isoforms. in a related species, ambystoma ... | 1987 | 2957262 |
| lymphocyte phenotypes at tumor margins in patients with head and neck cancer. | lymphocyte phenotypes were identified by monoclonal antibodies and avidin-biotin peroxidase reagents and enumerated in tumor tissue removed from 26 patients with head and neck cancer. t lymphocytes (t11) were the predominate phenotype at the tumor margin, with twice as many helper/inducer (t4) as suppressor/cytotoxic (t8) lymphocytes. patients with stage ii and iii carcinoma of the glottis, tongue, and hypopharynx had significantly increased numbers of all t lymphocyte phenotypes when compared t ... | 1987 | 2957343 |
| complexity of t cell receptor recognition sites for defined alloantigens. | three monoclonal antibodies react with the t cell receptor on the tumor line hpb-all and in addition with 3 to 13% of human peripheral blood t cells of normal donors. these antibodies are shown to react with an epitope encoded by the v beta 5 family of t cell receptor beta-chain variable region gene segments. cells expressing v beta 5 gene segments can have cytotoxic or helper function, be of the t4+ or t8+ phenotype, and have specificity for either class i or class ii major histocompatibility c ... | 1987 | 2957443 |
| phenotypic and functional analysis of t cells extracted from chronically inflamed human periodontal tissues. | t-cell subsets extracted from chronically inflamed periodontal tissues were identified using monoclonal antibodies, and their functional activity was analysed using the autologous mixed lymphocyte reaction (amlr). tissue was obtained from a total of 33 adult periodontitis (ap) patients and 6 normal/marginal gingivitis (n/mg) patients. all ap patients had received repeated oral hygiene instruction and root planing prior to the surgery, and the majority (30 out of 33) had at least one site with gr ... | 1987 | 2957490 |
| a major mechanism of human immunodeficiency virus-induced cell killing does not involve cell fusion. | in vitro studies indicate that human immunodeficiency virus (hiv) infections are cytopathic for t4+ peripheral blood lymphocytes and for most continuous lines of t4+ lymphocytes. these cytopathic effects have been largely attributed to the formation of syncytia by hiv-infected cells. we report that hiv infections killed cultured peripheral blood lymphocytes and a line of t4+-lymphoid cells (cem cells) without causing cell fusion. we also report that the occurrence of syncytia is an early and tra ... | 1987 | 2957508 |
| phenotypes of t lymphocytes from peripheral blood and synovial fluid of patients with rheumatoid arthritis and juvenile rheumatoid arthritis. evidence in favour of normal helper and suppressor functions of t lymphocytes from patients with juvenile rheumatoid arthritis. | lymphocytes from peripheral blood (pb) and synovial fluid (sf) from 21 patients with rheumatoid arthritis (ra) and 18 patients with juvenile rheumatoid arthritis (jra) were studied with respect to t cell phenotypes using monoclonal antibodies in a rosette assay. the percentage of hla-dr positive t cells was counted in pb and sf using indirect immunofluorescence. suppressor cell activity of t cells from pb and sf was investigated by measuring the immunoglobulin production by pokeweed mitogen (pwm ... | 1987 | 2957788 |
| lymphocyte subsets in urologic cancer patients. | peripheral blood t lymphocyte subsets were quantitated by monoclonal antibodies in 91 patients with urologic malignancies as well as eight age-matched controls. in general, the presence of cancer and increasing stage were associated with a relative decrease in helper and an increase in suppressor t-cell activity as well as a decreased t4/t8 cell ratio. therapy in the form of androgen depletion reversed these trends and restored helper and suppressor levels as well as the t4/t8 ratio to control v ... | 1987 | 2957843 |
| preoperative renal tumor embolization. a useful procedure? | the medical reports of 113 patients operated upon for renal tumors were retrospectively reviewed to assess the value of preoperative embolization. coils or ethanol were used preoperatively in 55 patients while 58 patients underwent nephrectomy without embolization. the tumors were staged according to the tnm and the robson staging classification. t1 and t2 tumors as well as t3 and t4 tumors were grouped together for statistical evaluation. there was no significant difference in intraoperative bl ... | 1987 | 2958037 |
| subpopulations of cd4+ (t4+) cells in homosexual/bisexual men with persistent generalized lymphadenopathy. | using monoclonal antibodies (anti-2h4 and anti-4b4), cd4+ (t4+) t cells are further subdivided into two major subpopulations of inducers of help (t4+4b4+) and inducers of suppression (t4+2h4+). to determine whether human immunodeficiency virus (hiv) infection results in the depletion of a subset of cd4+ cells, peripheral blood mononuclear cells from patients with persistent generalized lymphadenopathy (pgl) were quantified for the subpopulation of cd4+ t cells, using anti-2h4 and anti-4b4 monocl ... | 1987 | 2958183 |
| selective igm deficiency in adults: phenotypically and functionally altered profiles of peripheral blood lymphocytes. | peripheral blood lymphocytes from four patients with selective igm deficiency were examined phenotypically and functionally. although b cell subpopulations determined by surface immunoglobulins were within normal or nearly normal range, t8+ cells were significantly increased and t4/t8 ratios were inverted in three patients. igm specific hyporesponsiveness in the pwm-driven immunoglobulin production system was observed in all four patients. ia-like antigen positive t cells were increased in two p ... | 1987 | 2958191 |
| cd4 (t4+) lymphocytes in semen of healthy heterosexual men: implications for the transmission of aids. | 1987 | 2958370 | |
| antigen-induced human interferon-gamma production. differential dependence on interleukin 2 and its receptor. | once stimulated with toxoplasma gondii or cytomegalovirus (cmv) antigens, peripheral blood mononuclear cells from healthy seropositive donors secrete comparable levels of interferon-gamma (ifn-gamma). both antigens also stimulated specific production of interleukin 2 (il-2), a lymphokine believed to be important in ifn-gamma generation. t. gondii antigen, however, induced ninefold more il-2 than did cmv antigen suggesting different mechanisms for antigen-stimulated ifn-gamma production. therefor ... | 1987 | 2958545 |
| receptor-recognizing proteins of t-even type bacteriophages. constant and hypervariable regions and an unusual case of evolution. | proteins 38 of bacteriophages t2, k3, ox2 and m1 are located at the free ends of their long tail fibers and function as adhesins, i.e. they mediate binding to the bacterial receptors. the latter three phages use the escherichia coli outer membrane protein ompa as a receptor, while t2 uses the outer membrane proteins ompf or ttr. the dna sequences of genes 38 of phages ox2 and m1 have been determined and are compared with those known for t2 and k3. the genes encode 262(t2), 260(k3), 266(ox2) and ... | 1987 | 2958637 |
| immunoregulatory effects of intravenous immune serum globulin therapy in common variable hypogammaglobulinemia. | because of evidence of immunoregulatory effects of short-term administration of pooled donor gamma globulins, the effects of intravenous immune serum globulin were studied in nine adult patients with common variable hypogammaglobulinemia over a two-year period. baseline assessment prior to immune serum globulin replacement included evaluation of b cell function, suppressor cell activity, and t cell subsets. these analyses were subsequently performed during the course of a first-year treatment pe ... | 1987 | 2959150 |
| altered t4/t8 ratios in a patient with severe recurrent aphthous ulcers: report of a case. | 1987 | 2959760 | |
| abnormal lymphocyte reactivity to self-major histocompatibility antigens in rheumatoid arthritis. | proliferation of rheumatoid and control peripheral blood mononuclear cells to okt3, phorbol myristic acid (pma), phytohemagglutinin (pha), tuberculin ppd and in the autologous mixed lymphocyte reaction (amlr) was investigated. only the responses to ppd and in the amlr were depressed. this was not due to suppression by okt8 lymphocytes. the proportion of antigen responsive (t4+ 4b4+) cells was normal but suppressor-inducer (t4+ 2h4+) cells were decreased. the depressed response was not completely ... | 1987 | 2959772 |
| immunoregulatory t cells in alcoholic liver disease: phenotypical dissection of circulating leu3+/t4+ inducer t-lymphocytes. | alcoholic liver disease (ald) patients had normal absolute lymphocyte counts, increased percentage of leu3+/t4+ cells (p less than 0.001) and raised t4/t8 ratio (p less than 0.01). double-colour immunofluorescence analysis using isotype-specific goat anti-mouse immunoglobulins, fluorescein or rhodamine-conjugated, demonstrated that the rise in inducer (leu3+/t4+) t-cells was almost entirely represented by an expanded population of t4+tq1- and 5/9+ true helper lymphocytes. t4+ cells also expresse ... | 1987 | 2959783 |
| activities involved in base excision repair of bacteriophage t4 and lambda dna in vivo. | the in vivo excision repair functions of escherichia coli exonuclease iii and 3-methyladenine dna glycosylase i, and bacteriophage t4 pyrimidine dimer-dna glycosylase were investigated. following exposure of bacteriophage t4 or lambda to methyl methanesulfonate or ultraviolet irradiation, survival was determined by plating on e. coli have various genetic backgrounds. although exonuclease iii was shown to participate in base excision repair initiated by 3-methyladenine dna glycosylase i, it had n ... | 1987 | 2959841 |
| molecular approaches toward a therapy for multiple sclerosis. | treatment of experimental allergic encephalomyelitis with monoclonal antibodies to products of immune response genes and to surface markers on t cells was reviewed. both anti ia therapy and anti t4 therapy can reverse ongoing paralysis, and block subsequent relapses. the appearance of t4 cells and ia antigens in relapse was demonstrated. the prospects for human therapy with chimeric human-mouse immunoglobulins and isotypic variants was discussed, with particular attention to the immunotherapy of ... | 1987 | 2960007 |
| inhibition of streptomycin-dependent mutation in escherichia coli on the lytic growth of bacteriophage lambda. | spontaneous streptomycin-dependent mutants (strda) were isolated from escherichia coli c600. on c600 strd, the lytic growth of phage lambda nam and lambda ci857 was inhibited. after e. coli lysogenic strain 1.1485 (lambda ci857) mutated to strda, induction of lambda was decreased greatly. on strda of e. coli strains c600 and 1.1485 (lambda ci857), the plating efficiencies and burst sizes of phage t4 and t7 remained normal. since strda is a mutation in the structural gene for ribosomal protein s1 ... | 1987 | 2960017 |
| human t4+ t-lymphocyte clones specific for the b fragment of tetanus toxin. | two t4+ cloned t-lymphocyte lines specific for a papain digest product of tetanus toxin are functionally characterized. the two clones were obtained from peripheral blood mononuclear cells activated in vitro by tetanus toxoid, expanded with il-2, and cloned in soft agar. both clones could be induced to undergo blastogenesis with tetanus toxoid, tetanus toxin, and the b fragment but not the c fragment of tetanus toxin. in addition, both clones caused cytolysis of plastic adherent cell targets coc ... | 1987 | 2960640 |
| an open reading frame in the escherichia coli bacteriophage lambda genome encodes a protein that functions in assembly of the long tail fibers of bacteriophage t4. | assembly of the long tail fibers of the escherichia coli bacteriophage t4 requires the catalytic action of two auxiliary proteins. it was found that a gene of the entirely unrelated phage lambda codes for a protein which can substitute for one of these t4 polypeptides, protein 38. the lambda gene was designated tfa (tail fiber assembly). protein 38 consists of 183 residues, and the tfa protein consists of 194 residues; the two polypeptides are about 40% homologous. although the tfa gene is dispe ... | 1987 | 2960666 |
| plasma atriopeptin concentrations in hyperthyroidism, euthyroidism, and hypothyroidism: studies in man and rat. | atriopeptin (ap) is a polypeptide produced by atrial myocytes that is capable of inducing diuresis, natriuresis, and vasodilatation. because thyroid dysfunction is known to be associated with alterations in both renal function and vasomotor control, we investigate the possible effects of varying thyroid function on ap in humans and rats. plasma ap concentrations were determined in hyperthyroid and hypothyroid patients and normal subjects. plasma ap was also measured in some patients after the iv ... | 1987 | 2960689 |
| failure of t3 to potentiate tricyclic antidepressant response. | despite a lack of documented efficacy in controlled trials, triiodothyronine (t3) is frequently administered as an adjunctive therapy for tricyclic resistant depressions. in this study, we tested the efficacy of t3 as an adjunctive treatment using a double-blind, placebo-controlled crossover design. sixteen depressed patients who were unresponsive to 4 weeks of imipramine therapy (mean dose = 206 +/- 54 mg daily, mean combined blood level = 220 +/- 132 ng/dl) received t3 25 micrograms and placeb ... | 1987 | 2960719 |
| an antigen-specific t8+ human clone of cells with a nonspecific augmenting function on the t4 cell-b cell helper interaction. | we isolated a t8+ t3+ ia+ clone of cells from the peripheral blood mononuclear cells of a healthy subject. the clone was expanded and maintained with autologous feeder cells, interleukin 2, and a streptococcal antigen. the t8+ clone of cells responded specifically to the streptococcal antigen, in the absence of accessory cells, and released a soluble factor. both the cloned cells and the corresponding soluble factor expressed augmenting helper but not suppressor activity. the augmenting helper a ... | 1987 | 2960732 |
| the subdivision of the t4 (cd4) subset on the basis of the differential expression of l-c/t200 antigens. | the t4 (cd4) subset of t lymphocytes has been subdivided into two major subsets, a suppressor/inducer subset (t4+,2h4+) and a helper subset (t4+,2h4-) on the basis of the differential expression of the l-c/t200 (cd45) antigens. the 2h4 antigen itself comprises at least three distinct polypeptides at 125,200, and 220 x 10(3) mr, of which the 200 and 220 x 10(3) mr polypeptides constitute the highest mr isoforms of a pool of five distinct l-c/t200 antigens. the t4+,2h4+ subset expresses at least f ... | 1987 | 2960772 |
| prohead and dna-gp3-dependent atpase activity of the dna packaging protein gp16 of bacteriophage phi 29. | the atpase activity of the dna packaging protein gp16 (gene product 16) of bacteriophage phi 29 was studied in the completely defined in-vitro assembly system. atp was hydrolyzed to adp and pi in the packaging reaction that included purified proheads, dna-gp3 and gp16. approximately one molecule of atp was used in the packaging of 2 base-pairs of phi 29 dna, or 9 x 10(3) atp molecules per virion. the hydrolysis of atp by gp16 was both prohead and dna-gp3 dependent. gp16 contained both the "a-typ ... | 1987 | 2960820 |
| wild-type bacteriophage t4 is restricted by the lambda rex genes. | the bacteriophage t4 rii genes and the lambda rex (r exclusion) genes interact; rii mutants are unable to productively infect rex+ lambda lysogens. the relationship between rex and rii has been found to be quantitative, and plasmid clones of rex have excluded not only rii mutants but t4 wild type and most other bacteriophages as well. mutations in the t4 mota gene substantially reversed exclusion of t4 by rex. | 1987 | 2960831 |
| [structure and regulation of the assembly of bacteriophage t4 fibrillar proteins. i. isolation and spectral properties of long fibers]. | a preparative procedure for purification of the biological active proximal (a) and distal (bc') parts of bacteriophage t4 long-tail fibers is described. absorption spectra of these proteins in the near ultraviolet region were measured. the absorption coefficients were determined on the basis of the nitrogen content, the absorption coefficient for the a part is epsilon 0.1% 277 nm = 0.93 +/- 0.06 and for the bc' part is epsilon 0.1%, 277,5 nm = 1.01 +/- 0.08. calculations of the secondary structu ... | 1987 | 2960882 |
| [structure and regulation of the assembly of bacteriophage t4 fibrillar proteins. ii. isolation and initial structural characteristics of whiskers]. | a procedure for purification of bacteriophage t4 whiskers and it's monomeric subunits--gene product wac--has been developed. we have shown, that the whiskers are composed of two identical copies of gene product wac with molecular weight of 56 kda each. the dimer of gene product wac is a highly ordered structure and it's length is about 70.0 +/- 10.0 nm, as revealed by electron microscopy. the amino acid composition of whiskers is very similar to that of watersoluble keratins. we have proposed a ... | 1987 | 2960883 |
| defects of autologous mixed lymphocyte reaction-activated immunoregulatory t cells in patients with systemic lupus erythematosus. | the present study was undertaken to determine the nature of the immunoregulatory t-cell defect after autologous mixed lymphocyte reaction (amlr) activation in patients with systemic lupus erythematosus (sle). although amlr was decreased in patients with sle compared with normals, there was no difference in major proliferative cells (t4 cells and t4+jra+ subset) in response to amlr. functional activity of amlr-stimulated t4 subsets in patients with sle and normals was examined in helper and suppr ... | 1987 | 2961048 |
| t4-induced cardiac hypertrophy and the perfused and total microvasculature of the heart. | the purpose of the present investigation was to determine the effects of thyroxine (t4), which induces myocardial hypertrophy, on the number per square millimetre and volume per cubic millimetre of both the total and perfused portions of the arteriolar and capillary beds of the heart. studies were conducted in the subendocardial and subepicardial regions of the left ventricle of anesthetized open-chest rabbits. fluorescein isothiocyanate-dextran (i.v.) or radioactive microspheres (intra-atrial) ... | 1987 | 2961428 |
| hyaline-vascular type of castleman's disease (angiofollicular lymph node hyperplasia) with monotypic plasma cells. an immunohistochemical study with monoclonal antibodies. | a case of angiofollicular lymph node hyperplasia (castleman's disease) characterized by monotypic (igg+, lambda+) plasmacytosis is described. fresh tissue was available and a thorough immunohistochemical analysis of lymphoid and non-lymphoid cells was performed on cryostat sections. although lymphoid follicles were numerous and exhibited some abnormal features they did not appear part of the monoclonal cell proliferation. follicular lymphocytes were mixtures of kappa+ and lambda+ cells. vessels ... | 1987 | 2980702 |
| effects of amiodarone on thyroid hormone-responsive gene expression in rat liver. | the hypothesis that amiodarone (am) acts by inducing a local 'hypothyroid-like' state in thyroid hormone-responsive tissues was investigated in rat liver. hypothyroid rats, pretreated orally for 8 consecutive days with am (200 mg/kg) or water, were given a single i.p. injection of equimolar doses of t4, t3 or rt3 (1.00 to 1.20 mg/kg). six hours later, the rats were killed and liver nuclear t3 receptor occupancy was determined. simultaneously, the activity of two thyroid hormone-responsive enzyme ... | 1987 | 2895059 |
| non-hodgkin lymphoma in jamaica and its relation to adult t-cell leukemia-lymphoma. | of 95 patients consecutively diagnosed with non-hodgkin lymphoma, 52 (55%) had antibodies to human t-cell leukemia-lymphoma virus, type i. antibody positivity was strongly associated with skin involvement, leukemia, and hypercalcemia (p less than 0.02). two patients had systemic opportunistic infections. neither meningeal nor lung infiltration was detected, and lymph node infiltration was diffuse in all patients. of 36 patients who received immunophenotypic classifications, 30 had diseases that ... | 1987 | 2880536 |
| selective loss of the suppressor-inducer t-cell subset in progressive multiple sclerosis. analysis with anti-2h4 monoclonal antibody. | the t4+ lymphocyte population includes a subset that induces suppressor t lymphocytes (t8+ cells) and can be distinguished by dual-color fluorescence analysis with anti-2h4 and anti-t4 monoclonal antibodies. to investigate the possible role of these cells in multiple sclerosis, we used anti-2h4 antibody to characterize peripheral-blood lymphocyte subsets in 63 patients with multiple sclerosis that was progressive, stable, or acute (relapsing-remitting). twenty-three of 37 patients with progressi ... | 1987 | 2946956 |
| human anti-idiotypic t lymphocyte clones are activated by autologous anti-rabies virus antibodies presented in association with hla-dq molecules. | the regulatory function of antigen-specific t cells in human antibody responses to protein and carbohydrate determinants of many viral and bacterial antigens has extensively been studied in systems involving in vitro triggering of b cells by antigens or polyclonal activators. although amply documented in experimental murine models, the existence of t helper cells with receptor specificity for idiotypic determinants of b cell immunoglobulins has not been demonstrated in a human system. we are int ... | 1987 | 2908179 |
| ontogeny of human suppressor inducer t cell subset. | a t cell subset (t4+,2h4+) has recently been identified, which acts as an inducer of t8+ suppressor cells; however 2h4 molecules have not been detected in thymuses from normal individuals or on the surface of immature thymocyte clones. in the present study, t4+,2h4+ and t8+,2h4+ cells have been found in maternal and new-born blood samples. however, only a small percentage of 2h4+ cells were detected in human fetal thymus, bone marrow, liver and spleen, thus suggesting that t cells acquire these ... | 1987 | 2908511 |
| a family of autocatalytic group i introns in bacteriophage t4. | 1987 | 2841063 | |
| 32p-postlabeling assay for carcinogen-dna adducts: nuclease p1-mediated enhancement of its sensitivity and applications. | exceedingly sensitive assays are required for the detection of dna adducts formed in humans exposed to low levels of environmental genotoxicants and therapeutic drugs. a 32p-postlabeling procedure for detection and quantitation of aromatic carcinogen-dna lesions with a sensitivity limit of 1 adduct in 10(7) to 10(8) nucleotides has been described previously. in the standard procedure, dna is enzymatically digested to 3'-phosphorylated normal and adducted mononucleotides, which are 32p-labeled at ... | 1987 | 2834194 |
| evidence that mannosyl residues are involved in human immunodeficiency virus type 1 (hiv-1) pathogenesis. | the human immunodeficiency virus (hiv), the causative agent of aids, is thought to bind to t4+ (cd4+) target cells through the heavily glycosylated gp120 envelope glycoprotein. plant lectins bind glycoproteins through noncovalent interaction with specific hexose residues; therefore, lectins were evaluated for their ability to inactivate hiv in vitro. the mannose-specific lectins concanavalin-a and succinyl concanavalin-a completely inactivated hiv while lentil lectin, wheat germ agglutinin, and ... | 1987 | 2829950 |
| effects of human immunodeficiency virus (hiv) on the cytotoxic response to epstein barr virus (ebv) transformed b lymphocytes. | the human immunodeficiency virus (hiv) may interact with the epstein barr virus (ebv) indirectly by effects on the t4 lymphocyte or directly by effects on ebv transformed b lymphocytes. we have confirmed the susceptibility of ebv transformed b lymphocytes to productive hiv infection, and have evaluated the cytotoxic activity of hiv seronegative and seropositive donors after sensitization by their autologous ebv infected (monoinfected) or ebv and hiv infected (coinfected) transformed cell lines i ... | 1987 | 2829951 |
| [localization of canonical single-strand breaks in dna of the pseudomonas aeruginosa bacteriophage phi kf77]. | bacteriophages phi k of p. aeruginosa were characterized by the presence of t4 dna-ligase-repaired, single-chain breaks in their genome. a restriction map was constructed for one of these phages (phi kf77) with restriction endonucleases sali, hindiii, ecori, mlui, xbai and clai. phi kf77 dna was resistant to the cleavage by bamhi, bglii, hpai, psti, pvuii and xhoi endonucleases. single-chain breaks were mapped by means of electron microscopy of partially denatured dna molecules, electrophoretic ... | 1987 | 2833687 |
| thyroid activity in relation to feed intake, growth rate and feed conversion in growing heifers. | phenotypic and genetic parameters for thyroid activity (t4 serum level, t4 apparent distribution volume, t4 fractional turnover rate and t4 degradation), and for certain production traits (roughage dry matter intake, daily weight gain and roughage dry matter/kg weight gain), were investigated in cattle. in the experiment, 480 growing heifers were studied, daughters of 20 ai sires previously tested for thyroid activity. repeatabilities for thyroid traits 2-3 months apart varied, with an average 0 ... | 1987 | 2853665 |
| triiodothyronine effect on some parameters of carbohydrate metabolism in rat blood and liver. i. in vivo effect of triiodothyronine on liver glycogen content, blood metabolites and serum glycogenolytic activity. | short (7 days) t3-treatment has no influence on the plasma glucose and ffa concentration and blood lactate level, as well as on the hepatic glycogen content in the rats. the rise in t3 levels, observed in our study, was accompanied by a fall in t4 concentrations, indicating suppression of the endogenous t4 production. on the other hand, glycogenolytic activity of rat serum in vitro is the highest in the system: control liver slices-serum of rats pretreated with t3 as compared to the system: cont ... | 1987 | 2839007 |
| effect of thyroid hormones on the activity of hepatic glucose-6-phosphatase in fed and fasted rats. | the action of thyroid hormones on hepatic glucose-6-phosphatase was studied in rats. fed and 24-h fasted rats received t3 (10 micrograms/day) or t4 (25 micrograms/day) 1 h, 1 or 3 days before sacrificing. in addition a group of fed rats was treated with t4 for 7 and 14 days. the glucose-6-phosphatase activity was measured in the isolated microsomes prepared from the liver. the intactness of the microsomal preparation was checked using 2 mm mannose-6-phosphate as a substrate. in fed rats a single ... | 1987 | 2831125 |
| interaction of vipergic and cholinergic receptors in human thyroid cell. | the thyroid tissue is innervated by cholinergic and vipergic nerves. the present study investigated the possible interactions of cholinergic agents with vip-induced camp accumulation and thyroid hormone release in vitro. carbamylcholine (cch), acting through the muscarinic receptor increases cellular cgmp content in cultured human thyroid cells incubated with a phosphodiesterase inhibitor. cch (10 microm) inhibits cellular camp accumulation and thyroxine (t4) release induced by vasoactive intest ... | 1987 | 2829144 |
| predictive value of gallium scan, angiotensin-converting enzyme level, and bronchoalveolar lavage in two-year follow-up of pulmonary sarcoidosis. | evaluation of patients with pulmonary sarcoidosis with serum angiotensin-converting enzyme (ace), gallium scan, and bronchoalveolar lavage (bal) has proved useful in demonstrating active disease, especially in the lungs. long-term prognosis based on the results of pretreatment ace, gallium scan, and bal has not been previously clarified. we studied 44 patients with initially symptomatic pulmonary sarcoidosis who were begun on steroid therapy after initial evaluation. at 2 years, 21 of 44 (48%) p ... | 1987 | 2828772 |
| lymphocyte subsets and activation antigens in a reference population: a flow cytometric study using single and double antibody staining. | the transferrin receptor (tr), the hla dr antigen (dr), and the antigen binding okt10 (t10) are present on activated lymphocyte populations. the authors have studied their expression and that of antigens defined by eight commercial monoclonal antibodies on peripheral blood lymphocytes of 50 healthy hospital workers aged 23-60 years. a whole blood lysis technique was employed and cells were enumerated on a flow cytometer. the percentage of cells bearing the three activation antigens were generall ... | 1987 | 2828230 |
| receptor binding activity of lipid recombinants of apolipoprotein b-100 thrombolytic fragments. | apolipoprotein (apo) b-100, the protein constituent of low density lipoproteins (ldl), is the determinant responsible for ldl binding to the apob,e(ldl) receptor on cells. the current study was designed to identify the region(s) of apob-100 that interact with the apob,e(ldl) receptor. apolipoprotein b-100 was fragmented by thrombin digestion, and the isolated fragments (t2, t3, t4) were recombined with cholesterol-induced canine high density lipoproteins (hdlc). before the recombination, the rec ... | 1987 | 2828500 |
| processing and presentation of cell-associated varicella-zoster virus antigens by human monocytes. | to determine whether viral antigens associated with infected cells were processed for presentation to t cells, we cultured human blood mononuclear cells (mnc) from varicella-zoster virus (vzv) immune donors with vzv-infected fibroblasts of known hla type which had been fixed in 0.05% glutaraldehyde. after 7-8 days of culture thymidine uptake by t4+ cells exceeded that of t8+ cells. stimulated cells were depleted of adherent cells and restimulated with vzv-infected fibroblasts from donors matched ... | 1987 | 2826056 |
| molecular cloning and characterization of the gene encoding cholinephosphate cytidylyltransferase in saccharomyces cerevisiae. | 1. the structural gene for cholinephosphate cytidylyltransferase (cct) was isolated from a saccharomyces cerevisiae genomic library by means of complementation in a mutant of the yeast defective in the enzyme. the cloned dna restored both the growth and cholinephosphate cytidylyltransferase activity of the mutant. whereas the enzyme of the mutant was thermolabile, the enzyme produced by the transformant was indistinguishable in heat stability from that produced by the wild type. 2. strains carry ... | 1987 | 2826147 |