Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| surface marker studies on activated peripheral blood lymphocytes in idiopathic dilated cardiomyopathy. | expression of interleukin 2 (il-2) receptor on peripheral blood lymphocytes after stimulation with concanavalin a (con a) or anti-t3 monoclonal antibody was analyzed in patients with dilated cardiomyopathy (dcm) in order to clarify the abnormalities of lymphocyte activation mechanism in dcm. the expression of il-2 receptor after the stimulation with either con a or anti-t3 monoclonal antibody was found to be reduced in the patients with dcm when the results were compared with those of controls. ... | 1987 | 3112402 |
| mutations affecting translation of the bacteriophage t4 riib gene cloned in escherichia coli. | mutant ribosome binding sites of the bacteriophage t4 riib gene, resident on an 873 bp dna fragment, were cloned into a plasmid vector as in-frame fusions to a reporter gene, beta-galactosidase. the collection of mutations included changes in the region 5' to the shine/dalgarno sequence, a mutation of the shine/dalgarno sequence, the alternate initiation codons gug, aua and acg, and mutants in which several closely spaced initiation codons compete with each other on the same mrna. the results sh ... | 1987 | 3112515 |
| the secretory activity of the tuberoinfundibular dopaminergic neurons is modulated by the thyroid status in the adult rat: consequence on prolactin secretion. | an influence of thyroid status on the secretory activity of hypothalamic dopaminergic neurons was observed in adult rats and its involvement in the regulation of prolactin (prl) secretion was examined. the secretory activity of the tuberoinfundibular dopaminergic (tida) neurons was evaluated by measurement of dopamine (da) biosynthesis in the neurons and da release into hypophysial portal blood. the accumulation of da and prl in the adenohypophysis as well as prl concentration in plasma were als ... | 1987 | 3112600 |
| alterations in serum thyroid hormonal indices with colestipol-niacin therapy. | a serial blood-lipid-lowering study at the university of southern california yielded unexpected findings on routine thyroid function monitoring. after 1 year of combined colestipol and niacin therapy, patients had reduced total serum thyroxine (t4) levels and increased triiodothyronine uptake ratios, an indicator of apparent decreases in thyroxine-binding globulin levels. calculation of the free t4 index partially but not completely corrected for the apparent decrease in thyroxine-binding globul ... | 1987 | 3113309 |
| developing antler, a model for endocrine regulation of bone growth. concentration gradient of t3, t4, and alkaline phosphatase in the antler, jugular, and the saphenous veins. | thyroxine (t4), triiodothyronine (t3), and alkaline phosphatase (ap) were assayed monthly in white-tailed deer plasma obtained from the antler (a), jugular (j), and the saphenous (s) veins during the period of antler growth and the period of mineralization. the concentrations of t3, t4, and ap in the j and s were not significantly different in any month. during the most intense antler growth period (june and july) ap levels were significantly higher and t3 significantly lower in a as compared to ... | 1987 | 3113700 |
| lysis of fresh solid tumor targets in the presence of con a is mediated primarily by leu 7+ peripheral blood t lymphocytes: blocking by the anti-cd3 monoclonal antibody and comparison with recombinant interleukin 2-induced lysis by natural killer cells. | we investigated the lysis of fresh human solid tumor cells by peripheral blood t lymphocytes in the presence of lectins and anti-cd3 monoclonal antibodies (mab). addition of certain lectins (con a, pha, or wga) directly into the 4-hr 51cr-release assay caused significant lysis of (p less than 0.001) noncultured solid tumor targets by enriched populations of granular lymphocytes (gl). significant levels (p at least less than 0.001) of con a- or pha-dependent solid tumor lysis by gl-enriched lymph ... | 1987 | 3113741 |
| spontaneous human t-cell cytotoxicity against murine hybridomas expressing the okt3 monoclonal antibody: comparison with natural killer cell activity. | human peripheral blood lymphocytes (pbl) exhibited spontaneous cytotoxicity against okt3 monoclonal antibody (mab)-expressing murine hybridoma cells (okt3 hybridomas). in contrast, other murine hybridomas expressing okt4, okt8, anti-hla dr, and anti-hla a, b, and c mab were not lysed. pbl showed much lower levels of cytotoxicity (3 folds) against okt3 hybridomas as compared with nk activity against the k562 targets. lymph node (ln) cells exhibited the inverse relationship of cytotoxicity levels. ... | 1987 | 3113742 |
| use of whole blood methods in assessment of immune parameters in immunodeficiency states. | whole blood (wb) methods requiring less than 4 ml heparinized peripheral blood were used to define mononuclear cell phenotype, lymphocyte proliferation, and natural cell-mediated cytotoxicity (cyt) in samples from normal controls and patients with immunodeficiency states of acquired immunodeficiency syndrome (aids) and aids-related complexes (arc). results from two-color direct immunofluorescence staining of blood samples with monoclonal antibodies (mabs) conjugated to phycoerythrin (pe) or to f ... | 1987 | 3113759 |
| effect of thyroid hormone dosage on thyroid function tests in t4-replaced hypothyroid patients. | 1987 | 3113844 | |
| fast neutrons in the treatment of locally advanced breast cancer. | the clinical investigation of fast neutrons at hammersmith hospital included 17 patients who between them had 20 t4 breast cancers. the majority of these tumours were ulcerated and all were painful. ten had recurred after multiple other therapies. complete local regression was achieved in all but one (95%) and no tumour recurred. symptoms were relieved in all cases. median survival was 26 months. three patients developed small areas of skin necrosis following trauma of previous radiation. all th ... | 1987 | 3114006 |
| proliferation and immunoglobulin synthesis of peripheral blood mononuclear cells from patients with chronic liver disease stimulated by staphylococcus aureus cowan 1 and interleukin 2. | proliferation and igg synthesis of peripheral blood mononuclear cells (pbmc) in response to stimulation with recombinant interleukin 2 (il-2) and staphylococcus aureus cowan 1 (sac) were evaluated in 32 patients with chronic persistent hepatitis (cph), chronic active hepatitis (cah) and liver cirrhosis (lc). eleven patients had serum hbe antigen, 10 presented with hbe antibody and 11 had non-a, non-b hepatitis. igg synthesis of pbmc induced with the two stimuli was significantly decreased in pat ... | 1987 | 3114035 |
| serum thyroid hormone levels in patients with fulminant hepatitis: usefulness of rt3 and the rt3/t3 ratio as prognostic indices. | to evaluate thyroid function in 19 patients with fulminant hepatitis (fh), we have measured total and free 3,5,3'-triiodothyronine (t3) and thyroxine (t4), 3,3',5'-triiodothyronine (reverse t3, rt3), thyroid-stimulating hormone (tsh) and thyroxin-binding globulin (tbg) in patients with fh, compared with those of 80 patients with other various liver diseases and of 10 healthy controls. patients with fh showed the lowest values of serum t3 and the highest levels of rt3 among all patients with live ... | 1987 | 3114036 |
| increased gonadotropin levels in goldfish do not result in alterations in circulating thyroid hormone levels. | to determine whether increases in gonadotropin levels are capable of altering thyroid function in goldfish, plasma thyroid hormone levels were measured following induced changes in endogenous gonadotropin secretion and injection of carp gonadotropin. radio-frequency lesions placed in the nucleus preopticus periventricularis or monosodium-l-glutamate-induced lesions of the posterior nucleus lateralis tuberis (nlt) of the hypothalamus were capable of stimulating significant increases in plasma gon ... | 1987 | 3114043 |
| melatonin: its antagonism of thyroxine's antisomatotrophic activity in male syrian hamsters. | the effects of daily evening melatonin injections on serum and pituitary levels of growth hormone (gh) and follicle stimulating hormone (fsh) were investigated in male syrian hamsters receiving thiourea in the drinking water. melatonin injections, by themselves, had no significant effect on serum or pituitary gh. thiourea induced hypothyroidism reduced pituitary gh content but increased serum gh several fold. daily thyroxin (t4) injections for 3 weeks partially restored pituitary gh content and ... | 1987 | 3114053 |
| congenital thyroxine binding globulin deficiency: incidence and inheritance. | ninety-nine cases of thyroxine binding globulin (tbg) deficiency (90 males and 9 females) were identified among low-t4 infants after newborn hypothyroid screening. the data indicate that inherited tbg deficiency occurs in at least 1:5,000 newborns (1:2,800 males) and that mild and more pronounced forms are found in approximately equal proportions. genetic analysis indicates that x-linked inheritance is the usual mode with no suggestion of autosomal inheritance. | 1987 | 3114125 |
| long term follow-up of patients with cushing's disease treated by interstitial irradiation. | the first 86 patients with cushing's disease treated with interstitial irradiation (by needle implantation) as the sole therapy were reviewed. in the 82 patients who were reassessed 1 yr after treatment 63 (77%) achieved remission. this study comprises the outcome and complications in the 54 patients who had a remission and whom we were able to follow. the follow-up period ranged from 3-26 yr (mean, 10.5) from the time of remission. no instance of clinical or radiological relapse has occurred. o ... | 1987 | 3114299 |
| multiple sclerosis: relevance of class i and class ii mhc-expressing cells to lesion development. | expression of class i (hla-abc) and class ii (hla-dr; ia) major histocompatibility (mhc) antigens on endothelial cells and astrocytes was investigated in multiple sclerosis (ms) lesions of variable disease activity and in normal central nervous system (cns) using immunocytochemical techniques. findings were correlated to lesion pathology and to the presence and distribution of t cells, t cell subsets, and interleukin-2 (il-2) receptor-bearing cells. hla-abc was present on virtually all endotheli ... | 1987 | 3114324 |
| inhibition of the response of thyrotrophin to thyrotrophin-releasing hormone by thyroxine in hypothyroid rats treated with iopanoic acid: evidence suggesting an intrinsic biological activity of thyroxine without prior conversion to 3,5,3'-tri-iodothyronine. | the effects of a physiological replacement dose of thyroxine (t4) on the response of plasma tsh to trh in hypothyroid rats were studied. animals were treated with iopanoic acid (iop; 5 mg/100 g body weight) or vehicle 24, 12 and 1.5 h before the experiment. thereafter, 800 ng t4/100 g body weight were administered i.v. and 20 min later 1 microgram trh/100 g body weight was injected i.v. in control rats the basal concentration of tsh was 1450 +/- 300 (s.e.m.) mu./1. the plasma concentration of ts ... | 1987 | 3114404 |
| comparison of the effects of pulsatile and continuous trh infusion on tsh release in men. | pulsatile secretion of hypothalamic releasing factors modulates the release of pituitary hormones. to compare the effects of pulsatile and continuous administration of trh on tsh secretion, we studied six healthy euthyroid 20- to 38-year-old men by obtaining blood samples every 20 minutes for 12 hours (8 am to 8 pm) during five days of study. trh was administered according to the following schedule: day 1 (no trh, control); day 2 and subsequent day 3 (20 micrograms iv bolus of trh every 96 minut ... | 1987 | 3114594 |
| [[analysis of synergistic effects during thermoradiation exposure of bacteriophage t4 and bacillus subtilis spores]. | experimental data are interpreted in terms of a half-empiric model of synergism obtained for bacteriophage t4 and bacillus subtilis spores exposed to ionizing radiation of different dose rates at elevated temperatures. the model permits to optimize the ratio of both factors for effective sterilization. | 1987 | 3114821 |
| squamous cell carcinoma of the pyriform sinus treated with radical radiation therapy. | this is an analysis of 54 patients with squamous cell carcinoma of the pyriform sinus treated with radical irradiation at the university of florida between october 1964 and march 1984. there is a 2-year minimum follow-up on all patients and 85% have at least 5 years of follow-up. patients were excluded from analysis of disease control at the primary site, neck, and/or above the clavicles if they died less than 2 years from treatment with the site(s) continuously disease-free. all patients were i ... | 1987 | 3114832 |
| external beam irradiation for t1, t2-3 and t4 transitional cell carcinoma of the urinary bladder. | fifty-seven patients with t1 transitional cell carcinoma of the bladder, 57 patients with t2-3 and 10 patients with t4 were treated by radical irradiation +/- salvage cystectomy. thirty-five patients with t1 were treated by transurethral resection (tur) plus local chemotherapy +/- salvage cystectomy. eleven patients with t2-3 were treated with 5 x 4 gy in one week followed by cystectomy while four patients with t2-3 were treated with 40 gy in 4 weeks also followed by cystectomy. five patients wi ... | 1987 | 3114833 |
| effects of trh and grf administration on gh, tsh, t4 and t3 secretion in the lamb. | the effects of various amounts of thyrotropin-releasing hormone (trh) injected subcutaneously or intravenously (alone or in combination with growth hormone-releasing factor: grf 1-44) on growth hormone (gh), thyroid-stimulating hormone (tsh), thyroxine (t4) and triiodothyronine (t3) were studied in the plasma of 2-week, 2-month and 3-month old lambs. after subcutaneous trh administration, increases in plasma tsh, t4 and t3 levels were equivalent, whatever the amount of trh used (1,2,5 or 10 micr ... | 1987 | 3114838 |
| plasma somatotropin and somatomedin c concentrations following grf or trh injections in newborn calves. | plasma somatotropin (gh) and somatomedin c (igf1) concentrations were measured by radioimmunoassay in 3-dy old and 10-day old calves intravenously injected with growth hormone releasing factor (grf) 1-44, grf 1-29 or thyrotropin-releasing hormone (trh). in 3 day old animals the increase in plasma gh concentration was grf 1-44 dose-related (50, 100, 200 pmoles . kg(-1) of body wt). in four 10-day old calves injected with the lowest dose, the increase in plasma gh concentration was not different f ... | 1987 | 3114840 |
| the role of t4+ and ly-2+ cells in skin graft rejection in the mouse. | the role of the different t cell subsets (t4+ or ly-2+) cells in the rejection of murine skin grafts was examined using the in vivo injection of monoclonal antibodies. the rat antimouse monoclonal antibodies used were shown to be active in vivo and to successfully deplete t4+ or ly-2+ cells. h-2 mutant strains were used so that class i or class ii mhc antigens could be studied in isolation; non-h-2 antigenic differences were also examined. the results were clear: (a) for class i antigens, ly-2+ ... | 1987 | 3114924 |
| [detection of thyroid hormone antibodies using a radial immunodiffusion method]. | t3 and t4 autoantibodies just as protein binding anomalies are the cause of discrepancies of thyroid hormone assays as against actual thyroid function. depending on the separation techniques used, autoantibodies lead to false low or high t3 or t4 values. a series of 105 preselected thyroid patients was investigated as to the presence of t3 and t4 autoantibodies. only in one of these patients t3 autoantibodies could be documented as cause for elevated ft3 values in otherwise euthyroid function (c ... | 1987 | 3115039 |
| establishment of a common acute lymphoblastic leukemia cell line (lc4-1) and effects of phorbol myristate acetate (pma) on the surface antigen expression of the cell line. | a common acute lymphoblastic leukemia (all) cell line, designated lc4-1, was established from peripheral blood mononuclear cells of a patient with acute non-t-cell all. lc4-1 cells were characteristically positive for ia, b4, and common all antigens (calla), but negative for b2, tac, t3, t4, t8, t11, and m1 antigens and e-rosette formation. approximately 30% of lc4-1 cells expressed detectable amounts of b1 antigens. lc4-1 cells expressed neither epstein-barr-virus-associated nuclear antigen (eb ... | 1987 | 3115091 |
| establishment of an interleukin 2-dependent human t cell line from a patient with t cell chronic lymphocytic leukemia who is not infected with human t cell leukemia/lymphoma virus. | we established an interleukin 2 (il-2)-dependent human t cell line, kit 225, from a patient with t cell chronic lymphocytic leukemia (t-cll) with okt3+, -t4+, -t8- phenotype. southern blot analysis showed that kit 225 is not infected with human t cell leukemia/lymphoma virus (htlv) type i or ii, and is probably derived from the major clone in the fresh leukemic cells. kit 225 cells express a large amount of il 2 receptors constitutively and their growth is absolutely dependent on il 2. no other ... | 1987 | 3115332 |
| effect of thyroxine replacement therapy on plasma insulin-like growth factor 1 levels and growth hormone responses to growth hormone releasing factor in hypothyroid patients. | the aim of this study was to evaluate the effect of t4 replacement therapy on plasma insulin-like growth factor 1 (igf-1) levels in patients with primary hypothyroidism to see whether recovery of pituitary gh responsiveness to grf was associated with increased plasma igf-1 levels. igf-1 levels and gh responses to grf (1 microgram/kg) were measured in 21 patients with primary hypothyroidism before and after t4 replacement therapy. t4 increased plasma igf-1 levels (57.2 +/- 4.4 vs 75.9 +/- 8.8 ng/ ... | 1987 | 3115637 |
| the role of thyroid hormone as a biological amplifier of the actions of follicle-stimulating hormone in the functional differentiation of cultured porcine granulosa cells. | to characterize thyroid hormone action on the ovary, the direct effects of t4 or t3 were investigated in vitro using a monolayer culture system of porcine granulosa cells. monolayer cultures were maintained for 6 days in 4% serum-supplemented medium in the absence or presence of porcine fsh (20 ng/ml), with or without graded doses of t4 or t3. combined treatment with fsh and t4 (10(-7) m) induced morphological alternation resembling epithelioid cells, while fsh alone or t4 alone failed to bring ... | 1987 | 3115761 |
| dna ligase from drosophila melanogaster embryos. substrate specificity and mechanism of action. | dna ligase has been purified to homogeneity from 6-12 h drosophila melanogaster embryos (rabin, b. a., hawley, r. s., and chase, j. w. (1986) j. biol. chem. 261, 10637-10645). this enzyme had an apparent km for atp of 1.6 microm. of a variety of nucleotides tested, only adenosine 5'-o-(3-thio)triphosphate could substitute for atp in the joining reaction. the enzyme was competitively inhibited by datp, with an apparent ki of 2.3 microm. the apparent km for dna using p(dt)20 annealed with poly(da) ... | 1987 | 3115985 |
| thyroxine transfer and distribution in critical nonthyroidal illnesses, chronic renal failure, and chronic ethanol abuse. | serum t4 kinetic studies were performed in euthyroid patients with acute critical illnesses, chronic renal failure, or ethanol abuse without overt hepatocellular damage and in healthy euthyroid subjects with normal or altered serum t4 binding to determine the relative effects of altered serum t4 binding and extravascular disturbances on t4 transfer and distribution in nonthyroidal illnesses. a three-pool model with rapidly and slowly equilibrating pools exchanging with serum was used to evaluate ... | 1987 | 3116027 |
| reduced clearance rate of thyroxine-binding globulin (tbg) with increased sialylation: a mechanism for estrogen-induced elevation of serum tbg concentration. | hyperestrogenemic states, including pregnancy, cause an increase in serum t4-binding globulin (tbg) concentrations and an increase in the proportion of tbg molecules with greater anodal mobility on isoelectric focusing, indicating greater sialic acid content. the possible causal relationship between the degree of sialylation and accumulation of tbg in serum was explored by measuring the in vivo half-lives (t1/2) of tbgs with different isoelectric points. tbg in unfractionated serum and its major ... | 1987 | 3116030 |
| thyroid hormone modulation of thyrotrophin-releasing hormone (trh) and trh-gly levels in the male rat reproductive system. | thyrotrophin-releasing hormone (trh) occurs in high concentrations in the rat ventral prostate and its concentrations is regulated in a positive dose-response manner by testosterone in castrated rats. alpha-amidation of the tetrapeptide precursor, trh-gly, is a rate-limiting step in trh biosynthesis. to investigate further the hormonal regulation of trh biosynthesis in prostatic tissue, sprague-dawley rats of approximately 250 g were injected s.c. with either physiological saline or 3 mg propylt ... | 1987 | 3116140 |
| [immunologic surveillance of liver transplant patients using monoclonal antibodies against lymphocyte subpopulations]. | a precise immunological surveillance of liver graft recipients can allow the adaptation to each patient of an adequate immuno-suppressive treatment. in 11 liver-transplanted patients (7 primitive biliary cirrhoses, 2 post-hepatic cirrhoses, one bile duct atresia with one antitrypsin deficit) different lymphocyte subpopulations were tested before transplantation and at days 3, 5, 7, 15, 30, 60 and 120 after grafting using okt3, okt4, okt8 (orthoclone, france). in 6 patients presenting one or more ... | 1987 | 3116444 |
| thyroid-hypophyso-hypothalamic axis of the genetically hypoprolactinemic rats (ipl nude rats). | in order to evaluate thyrotropin function in the genetically hypoprolactinemic rat, (ipl nude), we measured by radioimmunoassay trh hypothalamic content, pituitary tsh content and serum tsh, t3, t4, both in ipl nude and control rats at various times over the 24-hour period. compared to normal rats, the hypothalamic trh content in the ipl nude rat showed similar variations during the day, whereas a slight increase was observed during the night characterized by a significant difference at 20.00 h. ... | 1987 | 3116445 |
| effects of vitamin a and/or thyroidectomy on liver microsomal enzymes and their induction in 2,3,7,8-tetrachlorodibenzo-p-dioxin-treated rats. | vitamin a and thyroid hormone status have been shown previously to alter the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) in rats. in the present study, we have examined the effects of a vitamin a-excess and a vitamin a-deficient diet on thyroid hormone levels, on selected drug-metabolizing enzymes in liver microsomes, and on their inducibility by tcdd in male sprague-dawley rats. except for a slight increase in serum t3 levels, none of these end points was affected by feeding rats the ... | 1987 | 3116724 |
| carcinoma of the subglottis. results of initial radical radiation. | during the 12-year period 1971 to 1982, 23 patients with primary squamous cell carcinoma of the subglottis were treated with initial radical radiation therapy. there were 19 men and four women, with a mean age of 64 years. five patients had t1 tumors, four had t2, three had t3, and 11 had t4. the actuarial overall and cause-specific survival rates were 26% and 61%, respectively. local control was achieved with initial radiation therapy in 16 patients (70%). subsequent local control was achieved ... | 1987 | 3117073 |
| time-course of alterations of high density lipoproteins (hdl) during thyroxine administration to hypothyroid women. | serum hdl cholesterol, apolipoproteins ai and aii and post heparin lipolytic activities (phla) have been measured in a group of fourteen hypothyroid women without ovarian oestrogen secretion before and during a 2-month thyroxine treatment. the more rapid and consistent observed event was a decrease in apo ai levels (164 +/- 5 vs. 149 +/- 5 mg dl-1, mean +/- sem, p less than 0.05) correlated (r = 0.79, p less than 0.05) to a slight increment of phla. a slight decrease in apo aii concentration was ... | 1987 | 3117568 |
| [the effect of serum free fatty acid on serum free thyroid hormone fractions in low t3 syndrome]. | we studied the role of serum free fatty acid (ffa) in the elevation of serum dializable fraction of t4 (dft4) and evaluated the serum free t4 (ft4) level in low t3 syndrome. serum dft4 and dft3 were measured by equilibrium dialysis method with phosphate buffer, and serum ffa concentration was obtained by gas chromatography. in patients with nonthyroidal systemic illnesses who showed reduced serum total t3 (tt3) and normal total t4 (tt4) (low t3 group, tt3 48 +/- 14 ng/dl, n = 10), mean (+/- sd) ... | 1987 | 3117601 |
| highly purified human t-lymphocytes do not respond to mitogens--including calcium ionophore and phorbol ester. | the proliferative, and other, in vitro responses of human lymphocytes are dependent upon interactions with accessory cells. studies of the nature of these required interactions are dependent upon the ability to isolate functionally pure populations of lymphocytes and accessory cells. we describe here a four-step separation procedure using plastic adherence, passage through columns of nylon wool and sephadex g-10, and treatment with l-leucine methyl ester which produced t-lymphocyte populations w ... | 1987 | 3117683 |
| purification and characterization of rna polymerase from the cyanobacterium anabaena 7120. | a procedure for the purification of rna polymerase from vegetative cells of the filamentous cyanobacterium anabaena 7120 is described. polyethyleneimine precipitation followed by gel filtration and affinity chromatography steps results in greater than 99% purification with 46% yield. the enzyme has a novel core component of mr = 66,000, designated gamma, in addition to the typical prokaryotic beta'beta alpha 2 core enzyme. the sigma subunit has been identified by reconstitution of specific trans ... | 1987 | 3117788 |
| t cell-derived blast crisis in chronic myelocytic leukemia. | we describe herein the occurrence of a t cell-derived blast crisis of chronic myelocytic leukemia which presented as a typical t cell leukemia on clinical (thymic mass and high white blood cells) and immunological grounds (t6+ t4+ t8+), with rearranged t cell receptor genes and germ line immunoglobulin genes. a philadelphia chromosome was detected in all blast cell mitoses. the significance of t cell involvement during the chronic and acute phases of chronic myelocytic leukemia is discussed. | 1987 | 3118106 |
| thyrotropin hyperresponsiveness to trh despite hyperthyroxinemia in amiodarone-treated subjects. | pituitary responsiveness to trh was assessed prospectively over 24 weeks, in 15 patients receiving 300 mg amiodarone a day. all developed significant hyperthyroxinemia (both total and free), and marked elevations in reverse t3 compared to pretreatment levels. although basal tsh levels were unchanged in all of them, tsh increased by greater than 50% when compared to pretreatment responses, in eight patients, while they remained unchanged (+/- 15%) in the remaining seven. all eight with exaggerate ... | 1987 | 3118137 |
| contributions of hydrogen bonds of thr 157 to the thermodynamic stability of phage t4 lysozyme. | measurements of changes in structure and stability caused by 13 different substitutions for threonine 157 in phage t4 lysozyme show that the most stable lysozyme variants contain hydrogen bonds analogous to those in the wild-type enzyme and that structural adjustments allow the protein to be surprisingly tolerant of amino-acid substitutions. | 1987 | 3118211 |
| hiv f/3' orf encodes a phosphorylated gtp-binding protein resembling an oncogene product. | apart from the retroviral gag, pol and env the hiv genome contains the f (3' orf) gene which encodes a polypeptide of 206 amino acids which is myristylated at the n-terminal and whose function is unknown. we have expressed the f gene in escherichia coli and from a recombinant vaccinia virus, vvtgfhiv. the f-protein produced in vvtgfhiv-infected mammalian cells is myristilated, and is phosphorylated by protein kinase c at a residue close to the n-terminus like pp60-src (ref. 5). purified bacteria ... | 1987 | 3118220 |
| thyrotropin response to thyrotropin-releasing hormone in rats with hypothalamic knife cuts. | regulation of the pituitary-thyroid axis in rats with hypothalamic knife cuts has been studied. complete hypothalamic deafferentation, either limited to the median eminence and the arcuate nucleus, or including parts of the dorsomedial nucleus and the whole ventromedial nucleus caused an increase in thyrotropin (tsh)-releasing hormone (trh)-induced tsh secretion. using an immunocytochemical procedure, a few trh-positive fibers were observed within the median eminence of the larger island, while ... | 1987 | 3118229 |
| effect of interferon gamma on expression of hla-class ii molecules on blood-derived dendritic cells. | t4 cells recognize antigen together with hla class ii molecules in the membrane of antigen-presenting cells (apc). the magnitude of the induced t cell response is in part dependent upon the quantitative expression of hla-class ii molecules in the cell membrane of the apc. we report here that recombinant interferon gamma (ifn-gamma) may enhance the expression of hla class ii molecules on the surface membrane of blood-derived dendritic cells by 30-90%. | 1987 | 3118639 |
| thyroid status in senile dementia of the alzheimer type (sdat). | thyroid function was investigated in a group of 21 patients with severe senile dementia of the alzheimer type (sdat) and in a group of 17 age and sex matched normal controls. free thyroid hormone levels (triiodothyronine (t3) and thyroxine (t4) were measured, as were also the thyrotrophin (tsh), prolactin (prl) and growth hormone (gh) responses to thyrotrophin releasing hormone (trh)). when compared to controls, patients demonstrated a significantly lower free t3 value (but not free t4), a blunt ... | 1987 | 3118642 |
| human blood basophils display a unique phenotype including activation linked membrane structures. | to evaluate the membrane marker profile of human basophils a panel of well-established monoclonal antibodies (moabs, n = 60) was used for a combined toluidine/immunofluorescence staining procedure. myeloid-associated moabs (particularly moabs against the lfa-1 family (cd11, cdw18), moabs directed against lactosylceramide (cdw17), anti-glycoprotein (gp) 150 moabs mcs 2 and my 7 (cdw13), anti-gp 67 moab my 9, anti fc gamma-receptor (mol wt 40 kd) moab cikm5, anti-cr 1 moab e 11, and the antiglycol ... | 1987 | 3118989 |
| repetitive or continuous thyrotropin (tsh)-releasing hormone administration induces a biphasic rise in plasma tsh in euthyroid but not in hypothyroid rats. | in euthyroid rats, repetitive bolus injections of 0.1, 1, or 5 micrograms/100 g bw trh every 15 min for 2 h produced a biphasic rise in the plasma tsh concentration. after an initial peak at 15 min, plasma tsh fell at 60 min to a nadir 50-80% of the 15-min peak, then rose again by 90 min to a plateau with approximately the same amplitude as the initial peak. plasma tsh remained at this level until trh injections were stopped at 2 h, then fell to the pre-trh baseline by 3 h. a similar biphasic ri ... | 1987 | 3119311 |
| galactorrhea in subclinical hypothyroidism. | galactorrhea was found in 5 patients with subclinical hypothyroidism. the galactorrhea consisted of the discharge of a few drops of milk only under pressure. serum t4 was in the lower level of the normal range, but serum t3 was normal (t4: 6.3 +/- 1.2 micrograms/dl, t3: 113 +/- 7 ng/dl). basal serum tsh and prl were slightly increased only in 2 and 1 cases, respectively. the prl responses to trh stimulation were exaggerated in all cases, although the basal levels were normal. an enlarged pituita ... | 1987 | 3119317 |
| monitoring treatment of congenital hypothyroidism by highly sensitive immunoradiometric assay for thyroid stimulating hormone. | to assess the clinical value of a sensitive immunoradiometric assay for tsh (irma-tsh), serum irma-tsh levels were compared with those of a radioimmunoassay (ria-tsh) in twenty-eight patients with congenital hypothyroidism. among 144 samples taken from them, 44 samples showed undetectable ria-tsh, while only 10 samples were undetectable by irma-tsh. in two patients prospectively followed, ria-tsh levels were undetectable when serum t3 and t4 were normal. irma-tsh levels, however, were detectable ... | 1987 | 3119318 |
| immunoelectron microscopic observations on the inflammatory infiltrates and hla antigens in hepatitis b and non-a, non-b. | the present knowledge of the inflammatory reaction occurring in situ during hepatitis b favors a t cell-dependent mhc-restricted immune response. however, the reports in the literature are primarily based on the application of monoclonal antibodies directed at different lymphocyte subsets which discern only lymphocytic phenotypes and do not reflect the actual situation adequately. therefore, we investigated the liver biopsies of patients with hepatitis b (28 patients) and non-a, non-b (21 patien ... | 1987 | 3119453 |
| endocrine function changes in young males during long-term antiepileptic therapy with phenobarbitone and carbamazepine. | the effects of long-term antiepileptic combined therapy with phenobarbitone (pb) and carbamazepine (cbz) on the major endocrine functions were evaluated in a selected group of 15 young males with partial epilepsy. the plasma basal levels of triiodothyronine (t3), thyroxine (t4), free thyroxine (ft4), thyrotropin (tsh), cortisol (co), dehydroepiandrosterone-sulfate (dhea-s), adrenocorticotropin (acth), growth hormone (gh), prolactin (prl) and testosterone (t) were determined. tsh and prl were als ... | 1987 | 3119517 |
| effects of plasmapheresis on peripheral blood mononuclear cell populations from patients with macroglobulinemia. | effects of plasmapheresis on peripheral blood t-cell, b-cell, monocyte, and natural-killer-cell populations were studied in ten macroglobulinemia patients with hyperviscosity syndrome. following plasmapheresis, there was a transient decrease in the number of t4+ helper cells and a longer-lasting decrease in the number of leu-7+ natural killer cells and mo2+ monocytes. in addition, there was a greater than 50% decrease in the in vitro ingestion capacity of monocytes. although no significant chang ... | 1987 | 3119575 |
| [a case report of an advanced ovarian cancer (stage t4) treated with a total amount of 1815 mg of cddp]. | a case is reported of a 34-year-old female who complained of an adult head-sized tumor in the pelvis and was treated with a high dose of cddp. on the first look operation, it was found impossible to perform reduction surgery because of severe adhesion and peritonitis carcinomatosa. to introduce a high concentration of an anti-cancer agent into the tumor tissue, we administered cddp intravenously, intraperitoneally and intra-arterially to the total amount of 1815 mg. after this high dose combinat ... | 1987 | 3119892 |
| bacteriophage t4 rega protein binds to mrnas and prevents translation initiation. | the bacteriophage t4 rega protein is a translational repressor of a subset of phage mrnas. we show here that purified rega protein binds specifically to target mrnas near the initiating aug and occludes binding of ribosomes. translational repression by rega protein diminishes expression of many genes whose mrna sequences around the initiating aug codons are different. a comparison of nucleotide sequences from several rega-repressed mrnas suggests that the initiating aug is an important, but not ... | 1987 | 3120177 |
| zinc (ii) and the single-stranded dna binding protein of bacteriophage t4. | the dna binding domain of the gene 32 protein of the bacteriophage t4 contains a single "zinc-finger" sequence. the gene 32 protein is an extensively studied member of a class of proteins that bind relatively nonspecifically to single-stranded dna. we have sequenced and characterized mutations in gene 32 whose defective proteins are activated by increasing the zn(ii) concentration in the growth medium. our results identify a role for the gene 32 protein in activation of t4 late transcription. se ... | 1987 | 3120192 |
| [the effect of beta-carotene on thyroxine and cholesterol levels in pregnant heifers before and after parturition]. | thyroxine (t4) and cholesterol concentrations and their dynamic changes and differences were studied in 20 pregnant heifers 10 days before calving until 56 days after calving. ten control heifers were fed the feed ration for pregnant heifers according to czechoslovak standard csn 46 7070. the feed ration given to another ten (experimental) heifers was fortified with 300 mg of synthetic beta-carotene per head/day within the period of five months before insemination, during pregnancy and till the ... | 1987 | 3120391 |
| handling of iodothyronines by the liver and kidney in patients with chronic liver disease. | possible arterio-venous gradients of t4, t3, rt3 and 3,3'-diiodothyronine (3,3'-t2) across the liver and the kidneys were measured in 9 patients with varying degrees of liver failure undergoing diagnostic catheterization. plasma iodothyronine levels were measured in peripheral, hepatic and renal veins before and at 10-min intervals until 60 min after iv injection of 400 micrograms of trh. in 2 patients estimated hepatic plasma flow and effective renal plasma flow were determined as well. in thes ... | 1987 | 3120447 |
| structure and function of a new hemoglobin variant, hb meilahti (alpha 2 beta 2 36(c2)pro----thr), characterized by mass spectrometry. | an abnormal hemoglobin was found by isoelectric focusing in the blood of a finnish woman with erythrocytosis. oxygen equilibrium curves of the patient's hemolysate indicated the presence of a variant with a very high oxygen affinity. structural analysis was carried out by mass spectrometry. in the spectrum of the tryptic digest, an abnormal peptide was found at m/z1278 which corresponded to the mass number of the protonated molecular ion of beta t4 with 36pro----thr substitution. the structure w ... | 1987 | 3120455 |
| post-operative analgesia by high thoracic epidural versus intramuscular nicomorphine after thoracotomy. part iii. the effects of per- and post-operative analgesia on morbidity. | one hundred and twenty-nine patients were subjected to three different types of thoracic operations. the patients were randomly allocated to balanced intravenous anaesthesia including i.v. nicomorphine during surgery and epidural nicomorphine post-operatively (epidural group, n = 58) or to balanced intravenous anaesthesia without i.v. opiates but with high thoracic epidural regional block during the operation and with post-operative intramuscular nicomorphine (intramuscular group, n = 71). post- ... | 1987 | 3120484 |
| abnormal sweat electrolytes in symptomatic human immunodeficiency virus infection in a child. | a 3 1/2 year old girl presented with failure to thrive and a five month history of diarrhoea and recurrent cough. the results of sweat sodium tests suggested a diagnosis of cystic fibrosis; but atypical organisms were found (haemophilus influenzae, candida albicans, but no staphylococcus aureus), she failed to respond to treatment, and her sweat sodium concentrations fell in response to fludrocortisone. she also had hyperglobulinaemia, neutropenia, and reduced numbers of t4 lymphocytes, which pr ... | 1987 | 3121056 |
| distribution of p24 htlv3 major core protein in lymph nodes of las patients. | lymph nodes of patients with lymphadenopathy syndrome (las) are characterized by two main histological patterns: hyperplastic reactive (h) and regressive (r). in both conditions, the paracortex (pc) is markedly activated with presence of selectively ia-1+ high endothelial venules. using a monoclonal antibody for p24, the major core protein of htlv3, we have immunohistochemically determined the distribution of p24+ cells in lymph nodes from 23 las patients' htlv3-ab serologically positive. p24+ c ... | 1987 | 3121180 |
| thyroid function in "yusho" patients exposed to polychlorinated biphenyls (pcb). | thyroid function was investigated in 123 yusho patients who were exposed to toxic levels of polychlorinated biphenyls (pcbs) 16 years ago. in yusho patients, compared with the patients without evidence of yusho or normal controls, the serum triiodothyronine (t3) and thyroxine (t4) levels were significantly higher, while thyroid stimulating hormone (tsh) levels measured by sensitive assay were normal. there was no difference in serum levels of albumin, alkaline phosphatase, total cholesterol, and ... | 1987 | 3121298 |
| australian hemophiliac recipients of voluntary donor blood products longitudinally evaluated for aids. a clinical and laboratory study, 1983-1986. | one hundred and sixty-one australian patients with hereditary bleeding disorders comprising hemophilia a (120), hemophilia b (18), von willebrand's disease (16), and seven symptomatic female hemophilia a or b carriers were screened for clinical and serological evidence of exposure to htlv-iii/lav/arv/hiv infection. during the previous five years (1979-1984) they had been treated almost exclusively with blood products derived from australian voluntary donors. the prevalence of htlv-iii antibodies ... | 1987 | 2963611 |
| action of long-chain fatty acids in vitro on ca2+-stimulatable, mg2+-dependent atpase activity in human red cell membranes. | human red cell membrane ca2+-stimulatable, mg2+-dependent adenosine triphosphatase (ca2+-atpase) activity and its response to thyroid hormone have been studied following exposure of membranes in vitro to specific long-chain fatty acids. basal enzyme activity (no added thyroid hormone) was significantly decreased by additions of 10(-9)-10(-4) m-stearic (18:0) and oleic (18:1 cis-9) acids. methyl oleate and elaidic (18:1 trans-9), palmitic (16:0) and lauric (12:0) acids at 10(-6) and 10(-4) m were ... | 1987 | 2963620 |
| [effect of 3,3',5' triiodo-l-thyronine (rt3) on the serum concentration of thyroid hormones in rats]. | 50, 100 or 150 micrograms/100 g body weight/day of very pure 3,3',5' triiodo-l-thyronine (rt3), obtained from a new synthetic method, was intraperitoneally administered in male wistar rats for 5 weeks. serum total thyroxine (t4), free thyroxine (ft4) and total 3,5,3' triiodo-l-thyronine (t3) concentrations were increased with all the doses of rt3. free t3 (ft3) was also but non-significantly elevated. different assumptions are put forward in order to explain this rt3 effect. | 1987 | 2963677 |
| [t cell subpopulations in peripheral blood and liver tissue in acute hbsag-negative hepatitis]. | by means of monoclonal antibodies against surface antigens of mononuclear cells we examined alterations of t-cell-subpopulations of peripheral blood and liver tissue in the course of acute hbsag-negative hepatitis. used methods for determination were the immunofluorescence for blood lymphocytes, respectively the peroxidase-anti-peroxidase-(pap)-technique for round-cell-infiltrates of the liver based on paraffin fixed sections. the focus of attention was the relation of t4-(helper/inducer) to t8- ... | 1987 | 2963738 |
| inhibition of thyrotropin-induced growth of rat thyroid cells, frtl-5, by immunoglobulin g from patients with primary myxedema. | we studied thyroid growth-blocking activity in immunoglobulin g (igg) fractions of serum from 24 patients with primary myxedema, 24 patients with goitrous hashimoto's thyroiditis, and 18 normal subjects by measuring the ability of their igg to inhibit tsh-induced [3h]thymidine incorporation into dna in a rat thyroid cell line, frtl-5. both groups of patients were receiving t4 when studied. [3h]thymidine incorporation induced by 0.1 mu/ml bovine tsh was significantly inhibited by the addition of ... | 1987 | 3023425 |
| loss of interleukin-2 requirement for the generation of t colonies defines an early event of human t-lymphotropic virus type i infection. | accessory cells and/or soluble factors, together with interleukin 2 (il2), are required for the proliferation and differentiation of phytohemagglutinin (pha)-activated t lymphocytes. human t-lymphotropic virus, type i (htlv-i), a human retrovirus isolated from patients with adult t cell leukemia, can transform t cells in vitro. we investigated the role of htlv-i-transformed t cell lines as accessory cells in promoting the growth of t colony-forming cells. we found that t cells isolated by e rose ... | 1987 | 3024755 |
| aids and antibodies to human immunodeficiency virus (hiv) in children and their families. | infection with human immunodeficiency virus (hiv, previously known as htlv-iii/lav) documented by a sensitive, specific immunoblotting (western blot) technique is described in 14 children with symptoms of aids or aids-related complex. for serodiagnosis of hiv infection, immunoblots blocked with milk were more sensitive than enzyme-linked immunosorbent assays or immunoblots blocked with gelatin. one or both parents of 13 of these children abused intravenous drugs. sixteen of 17 parents of the aff ... | 1987 | 3025309 |
| cyclic hormonogenesis in gray collie dogs: interactions of hematopoietic and endocrine systems. | cortisol, acth, t4, and t3 concentrations were determined in six cyclic hematopoietic (ch) dogs to determine if hormonal cycles were a feature of this hematopoietic disorder. it was determined that there were 12- to 14-day cycles of these hormones in ch dogs. plasma cortisol and acth concentrations peaked 4-8 days after the onset of neutropenia and were concurrent with peak neutrophil counts. the peak acth concentration occurred 1-2 days before or concurrent with the cortisol peak concentration. ... | 1987 | 3026784 |
| htlv-iii infection of ebv-genome-positive b-lymphoid cells with or without detectable t4 antigens. | the infection of a number of new and established b-cell lines by human t-cell lymphotropic virus iii (htlv-iii) was investigated. the b lymphocytes differed in their expression of t4 antigens detected by specific monoclonal antibodies (mabs) and the presence of epstein barr virus (ebv)-dna or antigens. the presence of the ebv genome was the only requirement for infection of b-lymphocytes by htlv-iii, although its presence did not ensure infection. two ebv genome and t4 antigen-positive b-cell li ... | 1987 | 3026971 |
| dephosphorylation of phosphoproteins and synthetic phosphopeptides. study of the specificity of the polycation-stimulated and mgatp-dependent phosphorylase phosphatases. | the substrate specificity of different forms of polycation-stimulated (pcsh, pcsl, and pcsc) phosphorylase phosphatases and of the catalytic subunit of the mgatp-dependent protein phosphatase from rabbit skeletal muscle was investigated. this was done, with phosphorylase a as the reference substrate, using the synthetic phosphopeptides patterned after the phosphorylated sites of pyruvate kinase (type l) (arg2-ala-ser(32p)-val-ala (s2), and its thr(32p) substitute (t4)), inhibitor-1 (arg4-pro-thr ... | 1987 | 3027075 |
| studies on the effect of chronic l-triiodothyronine (t3) treatment on brain na+,k+-atpase activity in the mature rat. | mature rats were dosed with t3 by different routes and dose-levels at either 0.1 mg/kg for 14 days s.c. (group a), 1 mg/kg for 3 alternative days i.p. (group b), 5 mg/kg for 14 days p.o. (group c), or with propylthiouracil (ptu 50 mg/day for 14 days p.o.-group d). measurement of cerebellar and striatal na+,k+-atpase activities showed that whereas groups a, b and d were unaffected when compared with controls, there were 35-70% increases respectively in the activities of both molecular forms of th ... | 1987 | 3027920 |
| lymphocyte phenotypes in cervical intraepithelial neoplasia and human papillomavirus infection. | lymphocyte phenotypes in cervical mucosa were studied using a panel of monoclonal antibodies. t lymphocytes were predominant both within the epithelium and in the subepithelial stroma. in the normal cervix, both the t4+ (helper/inducer) and t8+ (suppressor/cytotoxic) subsets were present in a ratio similar to that in the peripheral circulation. in human papillomavirus (hpv) infection and cervical intraepithelial neoplasia (cin) there was depletion of intraepithelial lymphocytes, especially of t4 ... | 1987 | 3028468 |
| detection in situ of active polypeptides of mammalian and t4 dna kinases after sodium dodecyl sulfate/polyacrylamide gel electrophoresis. | dna kinase activity of rat liver nuclei was detected in situ after electrophoresis in sodium dodecyl sulfate/polyacrylamide gel containing 5'-hydroxyl nicked dna as dna substrate. after renaturation of polypeptides, the gel was incubated with [gamma-32p]atp and mg2+. an active polypeptide corresponding to mr 61,000 was observed as a radioactive band by autoradiography. the intensity of the band was proportional to the amount of the enzyme applied. the active band common to various tissues of rat ... | 1987 | 3028786 |
| dna helicase ii of escherichia coli. characterization of the single-stranded dna-dependent ntpase and helicase activities. | escherichia coli helicase ii has been purified to near homogeneity from cells harboring a multicopy plasmid containing the structural gene for helicase ii, uvrd. in this paper a detailed description of the single-stranded dna-dependent nucleoside 5'-triphosphatase and helicase reactions catalyzed by helicase ii is presented. the results of this study suggest that nucleoside 5'-triphosphate hydrolysis provides the energy required for translocation of the enzyme along single-stranded dna. measurem ... | 1987 | 3029063 |
| formation of rolling-circle molecules during phi x174 complementary strand dna replication. | the primosome is a mobile multiprotein priming apparatus that requires seven escherichia coli proteins for assembly (the products of the dnab, dnac and dnag genes; replication factor y (protein n'); and proteins i, n, and n"). while the primosome is analagous to the phage t7 gene 4 protein and phage t4 gene 41/61 proteins in its dna g-catalyzed priming function, its ability to act similarly also as a dna helicase has remained equivocal. the role of the primosome in unwinding duplex dna strands w ... | 1987 | 3029072 |
| a specific assay measuring binding of 125i-gp 120 from hiv to t4+/cd4+ cells. | the hiv (htlv-iii) envelope glycoprotein, gp120, was isolated from virus-infected tissue culture cells using affinity chromatography. a radioimmunoassay was developed to determine the degree of iodinated gp120 to target cd4+ (t4+) cells. 125i-gp120 could be shown to selectively bind to cd4+ cells only. the gp120 remained bound to these cells after repeated washes. monoclonal anti-cd4 antibodies block the binding of gp120 to cd4+ cells. monoclonal antibodies to other cell surface components do no ... | 1987 | 3029227 |
| specificity and efficiency of editing of mismatches involved in the formation of base-substitution mutations by the 3'----5' exonuclease activity of phage t4 dna polymerase. | the specificity and efficiency of base-mispair editing by the 3'----5' exonuclease activity of phage t4 dna polymerase has been measured using a sensitive infectivity assay. a series of oligodeoxynucleotide primer chains was synthesized chemically. these primers, when hybridized to phi x174 single-stranded dnas containing an amber codon, result in a mispaired nucleotide at the 3'-hydroxyl end of the primer chain within the amber codon. dna synthesis on these primer x templates without the remova ... | 1987 | 3029763 |
| proton nmr measurements of bacteriophage t4 lysozyme aided by 15n isotopic labeling: structural and dynamic studies of larger proteins. | a strategy for resolution and assignment of single proton resonances in proteins of molecular mass up to at least 40 kda is presented. this approach is based on 15n (or 13c) labeling of selected residues in a protein. the resonances from protons directly bonded to labeled atoms are detected in a two-dimensional 1h-15n (or 13c) spectrum. the nuclear overhauser effects from isotopically tagged protons are selectively observed in one-dimensional isotope-directed measurements. using this approach, w ... | 1987 | 3029773 |
| molecular characterization of the cell cycle-regulated thymidylate synthase gene of saccharomyces cerevisiae. | the complete nucleotide sequence of a 1.8-kilobase dna fragment containing the cell cycle-regulated thymidylate synthase gene (tmp 1) of the yeast saccharomyces cerevisiae is presented. this analysis has revealed a 912-base pair open reading frame which encodes a 304-amino acid residue protein with a calculated mr of 35,007. the tmp1-6 and cdc21-1 mutant alleles of this gene also have been sequenced, and both show single base pair changes which would result in different amino acid substitutions. ... | 1987 | 3031048 |
| donor age-dependent decline in response of human red cell ca2+-atpase activity to thyroid hormone in vitro. | the effect of increasing donor age on the susceptibility of human red blood cell ca2+-atpase activity to stimulation in vitro by thyroid hormone was studied in 26 normal subjects, aged 15-81 yr. basal enzyme activity (no added thyroid hormone) was unaffected by donor age. group analysis, young (less than or equal to 50 yr) vs. elderly (greater than 60 yr old), revealed a 23% decrease in responsiveness of the enzyme to l-t4 (p less than 0.001). regression analysis confirmed an age-dependent decli ... | 1987 | 3031121 |
| effects of human immunodeficiency virus on the cellular immune response to epstein-barr virus in homosexual men: characterization of the cytotoxic response and lymphokine production. | we investigated epstein-barr virus (ebv)-specific t cell responses in homosexual men with, and at risk for, aids. we studied healthy laboratory workers, healthy homosexual men, and patients with aids-related complex or aids. the cytotoxic activity, absolute number of t4 lymphocytes, and interleukin-2 (il-2) production decreased, whereas the relative number of ia+ lymphocytes increased with the extent of infection with the human immunodeficiency virus (hiv). cytotoxic activity correlated positive ... | 1987 | 3031170 |
| determination of thyroxine, triiodothyronine, and cortisol changes during simultaneous adrenal and thyroid function tests in healthy dogs. | changes in thyroxine (t4), triiodothyronine (t3), and cortisol during a combined adrenal (dexamethasone suppression/adrenocorticotrophic hormone response test) and thyroid function tests (thyroid-stimulating hormone [tsh] response test) were determined in 20 healthy hospitalized pet dogs. the effect of dexamethasone on t4 and t3 changes was evaluated during a simultaneous tsh response/dexamethasone suppression adrenocorticotrophic hormone response test. greater ranges in basal cortisol concentra ... | 1987 | 3032026 |
| long-term observations of the patterns of failure in patients with unresectable non-oat cell carcinoma of the lung treated with definitive radiotherapy. report by the radiation therapy oncology group. | this report details the patterns of tumor recurrence in two prospective randomized studies involving 551 patients with histologically proven unresectable or medically inoperable non-oat cell carcinoma of the lung treated with definitive radiotherapy. patients were treated according to two protocols, depending on the stage of the tumor: (1) patients with t1, 2, 3-no, 1, 2 tumors were randomized to four different regimens: 4000 cgy split course (2000 cgy in five fractions, per 1 week, 2 weeks rest ... | 1987 | 3032394 |
| role of postnatal gonadal function in the determination of thyrotropin (tsh) releasing hormone-induced tsh response in adult male and female rats. | permanent effects of postnatal gonadal function on the hypothalamo-pituitary-thyroid axis were examined in male and female rats of the wistar-imamichi strain. animals were used at the age of about 10 weeks. neonatally castrated (nc) males showed a significantly higher plasma tsh response to trh (10 micrograms/kg bw, ip) than males castrated at 1, 2, 3, 4, or 6 weeks of age. the tsh response in intact males was similar to that seen in nc males. neonatal androgenization of nc males with 100 microg ... | 1987 | 3032567 |
| [plasmid vectors of "insertion inactivation" of the trimethoprim resistance marker]. | we demonstrate the possibility of using the t4 phage frd gene as an insertion inactivation marker within pbr322, in plasmids with changing copy number and expression of foreign genes under control. the structural part of the frd genes contains unique recognition sites for ecori and sali endonucleases. transformants with recombinant plasmids carrying the frd gene grow on media with up to 500 mkg/ml trimethoprim, whatever the gene dosage. | 1987 | 3032740 |
| the significance of antilymphocyte antibodies in patients with acquired immune deficiency syndrome (aids) and their sexual partners. | antilymphocyte antibodies have been demonstrated in autoimmune diseases, acute viral infections, and acquired immune deficiency syndrome (aids) by using either the conventional microlymphocytotoxicity or the double fluorescence technique. in the present study, we used both methods to detect the antilymphocyte antibodies and to characterize further their immunologic significance in patients with aids and their sexual partners. the results using the conventional microlymphocytotoxicity method demo ... | 1987 | 3033011 |
| thyroid effects on adenosine 3',5'-monophosphate levels and adenylate cyclase in cultured neuroblastoma cells. | using neuroblastoma cells as a model of developing neurons, we have tested the hypothesis that thyroid hormones alter camp metabolism. neuroblastoma cells were grown in serum-free defined medium for 48 h with or without thyroid hormones. treatment with 20-200 nm 3,5,3'-triiodo-l-thyronine (t3) increased the accumulation of camp by intact cells without altering growth, gross morphology, or dna or protein content. the increase in camp accumulation could be detected 5 h after the addition of t3 and ... | 1987 | 3030693 |
| regulatory effect of lithium on thyroxine metabolism in murine neural and anterior pituitary tissue. | the conversion of t4 to t3 in the brain and anterior pituitary gland contributes significantly to the t3 content of these tissues and appears to be an important modulator of thyroid hormone action. in the present study, the antimanic agent lithium was demonstrated in cultured neural and pituitary tissue to have a significant inhibitory effect on the activity of low km (type ii) iodothyronine 5'-deiodinase (i5'd), the enzyme mediating t3 formation. at medium lithium concentrations of 3.3-5 mm, 15 ... | 1987 | 3030700 |
| cytomegalovirus infection in homosexual men. relationship to sexual practices, antibody to human immunodeficiency virus, and cell-mediated immunity. | to investigate the relationships among cytomegalovirus infection, sexual behavior, human immunodeficiency virus (hiv) seropositivity, and indexes of cellular immunity, 180 homosexual men and 26 heterosexual men were studied. among the homosexual men, cytomegalovirus seropositivity was associated with increased t8 lymphocyte counts (p less than 0.001) and reduced t4/t8 ratios (p = 0.006); these results were independent of hiv infection. cytomegalovirus seropositivity was also associated with incr ... | 1987 | 3030101 |
| inflammatory demyelinating peripheral neuropathies associated with human t-cell lymphotropic virus type iii infection. | nine patients with inflammatory demyelinating polyneuropathies (idp) were found to have human t-cell lymphotropic virus type iii (htlv-iii) infection. the 8 men, 6 of whom were homosexual, and 1 woman, a former intravenous drug user, presented with progressive weakness. two had lymphadenopathy but all were otherwise asymptomatic. six had chronic idp and 3 had guillain-barré syndrome. in addition to an elevated cerebrospinal fluid (csf) protein level (mean, 193 mg/dl), most patients had cerebrosp ... | 1987 | 3030188 |
| potential anti-aids drugs. 2',3'-dideoxycytidine analogues. | 5-substituted 2',3'-dideoxycytidine analogues have been synthesized and evaluated in vitro for their capabilities to protect t4+ lymphocytes from the cytopathic effects of the htlv-iii/lav (hiv) virus, the causative agent of acquired immunodeficiency syndrome (aids). these analogues were designed to be more lipophilic than 2',3'-dideoxycytidine (ddc) in order to enhance central nervous system penetration. earlier reports had shown that ddc is a potent protective agent. when ddc is substituted at ... | 1987 | 3033243 |
| thyroid hormone increases rat atrial natriuretic peptide messenger ribonucleic acid accumulation in vivo and in vitro. | thyroid hormone has a number of effects on cardiovascular and renal function which are shared by the atrial natriuretic peptide (anp). we attempted to demonstrate a relationship between the two by studying the effects of thyroid hormone on the expression of the anp gene and the secretion of its encoded protein. thyroid hormone, when given to thyroidectomized rats, increased plasma anp levels by approximately 2-fold in both watered and dehydrated animals. cardiac anp mrna in dehydrated animals fe ... | 1987 | 2969452 |
| studies of lymphocyte subpopulations and immunoglobulin production in iga nephropathy. | this work was undertaken to examine the immunoregulation of t lymphocytes in patients with iga nephropathy. fifty patients and thirty-seven control subjects were studied in an infection-free interval. t lymphocyte subpopulations were determined using okt monoclonal antibodies against helper (okt4) and suppressor (okt8) t cell subsets. the proportions of t lymphocyte subpopulations did not differ between patients and controls. patients with significant renal impairment demonstrated a reduced okt4 ... | 1987 | 2964968 |
| cell-populations involved in the humoral leukocyte adherence inhibition reaction. the t8-suppressor/cytotoxic cells are both effectors and responders. | in an attempt to identify the cell subpopulation involved in the humoral leukocyte adherence inhibition (h-lai) reaction, mononuclear leukocytes were fractionated and the response with the different cell populations was measured in the h-lai assay using sera from lung cancer patients and a lung cancer associated antigen. the monocytes were found to exhibit a suppressive effect on the h-lai response. the b-lymphocytes seemed not to take an active part in the reaction. depletion of the t-lymphocyt ... | 1987 | 2965016 |