Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [research related to hormones in nagase analbuminemic rats]. | the levels of hormones in the anterior pituitary gland and serum in nagase analbuminemic rats (nar) were examined. for the anterior pituitary gland, the contents of prolactin, tsh, gh, lh and fsh in male nar were lower than those of normal rats. in female nar, prolactin and tsh levels were also lower than those in normal rats, whereas lh, fsh and gh were unchanged. in serum, the concentrations of tsh, t3, t4, estradiol, testosterone and prolactin (female only) were examined. the serum prolactin ... | 1987 | 3105460 |
| immune status of greek patients with beta-thalassemia major negative for anti-hiv. | patients with thalassemia who receive multiple blood transfusions are at risk for the acquired immunodeficiency syndrome. peripheral blood lymphocyte subpopulations were studied in 22 multitransfused thalassemic patients; 10 patients were without splenectomy and 12 were studied after splenectomy. both groups were negative for anti-hiv. four additional patients who were found positive for anti-hiv and ten healthy controls were also included in this study. patients without splenectomy compared to ... | 1987 | 3105626 |
| free t4 measurement is preferred to the t4 test for thyroid evaluation in lithium treated patients. | free t4 (ft4) measurement provides a more sensitive test of thyroid function than the commonly used t4. recent commercial development of ft4 kit assays has made ft4 assessment widely available. evaluation of the ft4 amerlex kit in 26 lithium-treated patients illustrates its utility as a sensitive laboratory test of thyroid function in this group of patients. this finding together with ft4's known superiority over t4 in identifying thyroid dysfunction suggests that ft4 should replace t4 estimatio ... | 1987 | 3105860 |
| thyroid hormone-binding proteins in plasma facilitate uniform distribution of thyroxine within tissues: a perfused rat liver study. | we used autoradiography to test the hypothesis that a major function of thyroid hormone-binding proteins in plasma is to ensure uniform distribution of thyroid hormones among cells of a given tissue. the distribution of [125i]t4 within rat hepatic lobules was determined after its single pass perfusion through the portal vein in solutions containing or lacking thyroid hormone-binding proteins. these proteins included thyroid hormone-binding globulin, thyroid hormone-binding prealbumin, and albumi ... | 1987 | 3106010 |
| childhood lead toxicity and impaired release of thyrotropin-stimulating hormone. | decreased stature of children is epidemiologically associated with increased blood lead independent of multiple socioeconomic and nutritional variables. since endocrine dysfunction occurs in adult lead workers, we studied two girls, 2 years of age, before and after calcium disodium edetate chelation for blood leads (pbb) of 19-72 micrograms/dl. the height of both children had crossed from the 50th to below the 10th percentile during the course of chronic lead toxicity. basal free t4, t4, t3, cor ... | 1987 | 3106028 |
| human mucosal cytotoxic effector cells. | human intestinal lamina propria mononuclear cells have been shown to mediate mitogen-induced cellular cytotoxicity, antibody-dependent cellular cytotoxicity, and lymphokine activated killer cell function. however, although natural killer cells have been demonstrated in the gut mucosa of rodents, recent reports found little or no spontaneous cytotoxic activity in the lamina propria of the human gut. using the natural killer cell-related monoclonal antibody nkh-1, which has not previously been app ... | 1987 | 3106129 |
| reversal of advanced murine lupus in nzb/nzw f1 mice by treatment with monoclonal antibody to l3t4. | murine lupus in nzb/nzw f1 (b/w) mice can be prevented by weekly treatment with monoclonal antibodies (mab) to l3t4 (on "helper/inducer" t cells) if treatment is begun prior to the onset of clinical illness. to determine whether anti-l3t4 could reverse as well as prevent murine lupus, we monitored a cohort of 30 b/w females until age 7 mo, when severe autoimmune disease was established, and then we examined the effects of weekly treatment with mab to l3t4. the rate of target cell clearance by ma ... | 1987 | 3106478 |
| variability of l-thyroxine replacement dose in elderly patients with primary hypothyroidism. | sixty-three elderly patients (aged more than 65 years) who manifested primary hypothyroidism during 4 1/2 years from september 1980 to march 1985 were studied. patients were divided into two groups depending on the presence or absence of chronic associated disorder at the time of diagnosis. patients in the sick group were required to consume several medications throughout the study period in addition to l-thyroxine for their total therapeutic management. subjects in the healthy group required l- ... | 1987 | 3106558 |
| [the hypothalamo-hypophyseal thyroid axis, plasma protein concentrations and the hypophyseo-gonadal axis in low t3 syndrome following acute myocardial infarct]. | in patients with severely acute diseases, a special relationship of thyroidal hormones with decreased t3 and increased rt3 levels is known, the so-called low t3 syndrome. the aim of this study was to elucidate the involvement of the hypothalamo-pituitary thyroid axis, the pituitary-gonadal axis, the altered hepatic function, the plasma proteins in the low t3 syndrome, and the evaluation of these parameters for prognosis in patients with acute myocardial infarction. thirty-one patients (29 male, ... | 1987 | 3106708 |
| hyperthyroidism and high serum levels of tsh associated with pituitary tumour. | we report a 28-year-old male with persistent clinical and laboratory findings of hyperthyroidism associated with marked elevated serum levels of tsh and no response to trh despite hemithyroidectomy and subsequent antithyroid drug therapy two years previous to admission to our hospital. subsequent skull x-rays, ct scans and angiographic findings demonstrated the presence of a pituitary tumour. after operation and radiation therapy t3, t4 and tsh levels returned to normal values. seven years later ... | 1987 | 3106846 |
| effects of microwaves on three different strains of rats. | confounding factors influencing the sensitivity of biological indicators of microwave exposure--lethality, colonic temperature (tco), decreased body mass (dw), corticosterone (cs), thyrotropin (tsh), thyroxine (t4), free thyroxine (ft4), and prolactin (prl) concentration--were studied in long-evans (le), wistar-kyoto (wky), and spontaneous hypertensive (shr) rats. the microwave signal was 2.45 ghz amplitude modulated at 120 hz. test power density ranged from 1 to 50 mw/cm2 for 2 h. in contrast t ... | 1987 | 3107046 |
| human immunodeficiency virus infection of monocytic and t-lymphocytic cells: receptor modulation and differentiation induced by phorbol ester. | the monocytic leukemic cell line u937 can be infected with human immunodeficiency virus type 1 (hiv-1) to become permanently infected virus producers. uninfected u937 cells express t4 (cd4) antigen and form syncytia when mixed with hiv-1 producing cells. anti-t4 monoclonal antibodies block syncytium formation indicating that the hiv-1 receptors on u937 cells include t4 antigen. the promyelocytic leukemic cell line hl60, while expressing only low amounts of surface t4 and not forming syncytia on ... | 1987 | 3107214 |
| serum free thyroid hormones in different degrees of hypothyroidism and in euthyroid autoimmune thyroiditis. | serum total and free t4 and t3, thyroxine-binding globulin (tbg) and tsh, basal and 20, 30 and 60 min after trh (200 micrograms, iv), were evaluated in 125 hypothyroid patients (38 with severe, 23 with mild, and 64 with subclinical hypothyroidism), in 35 euthyroid subjects with autoimmune thyroiditis, and in 51 healthy controls. t4/tbg and t3/tbg ratios were also calculated. a significant decrease in all indices of thyroid function except for t3 occurred simultaneously with a significant increas ... | 1987 | 3107297 |
| cytochemistry of n-acetyl-beta-glucosaminidase in normal and leukemic t cells. | a cytochemical study of the n-acetyl-beta-glucosaminidase (nabg) activity in normal and leukemic t cells was carried out to ascertain any relationships between cytochemical reactivity and membrane phenotype. under normal conditions, most t4-positive cells, defined on the basis of monoclonal antibodies and immunogold, were characterized by focal reactivity. in chronic t lymphoproliferative disorders, the reaction product of t4-positive cells consisted of a single coarse granule, while t8-positive ... | 1987 | 3107326 |
| prolactin in cluster headache: diurnal secretion, response to thyrotropin-releasing hormone, and relation to sex steroids and gonadotropins. | the diurnal rhythmicity of serum prolactin (prl) and the prl and thyrotropin (tsh) response to thyrotropin-releasing hormone (trh) were studied in 31 cluster headache patients (4 chronic cases) and 14 healthy controls. sixteen of the patients were studied both during clinical remission and headache periods. in males the nocturnal prl peak was blunted during remissions as compared with that in cluster periods and that in control individuals. the 24-h mean prl levels were lower during remission an ... | 1987 | 3107837 |
| changes in lh and fsh serum pattern following l-t4 withdrawal in l-t4 substituted hypothyroids. | 1987 | 3108132 | |
| heat treatment for endocrinological investigations on plasma positive for human immunodeficiency virus (hiv). | the effects of heat treatment of serum samples on the hormone analyses used in this laboratory were studied. total t4, testosterone, progesterone, and growth hormone were not systematically affected by heat treatment over the whole range of analyte concentrations studied; for thyroid stimulating hormone, no effect was noted on serum samples with concentrations of less than 10 mu/l. significant changes occurred in total t3, cortisol, follicle stimulating hormone, luteinizing hormone, and prolacti ... | 1987 | 3108328 |
| pituitary-hypothalamic response in adolescents with growth failure due to fear of obesity. | nine patients (4f, 5m) aged 12-17 years with "fear of obesity" were studied with a sequential stimulation test utilizing insulin, lrh, trh, and l-dopa. the comparative groups were nine female with classic anorexia nervosa, five males with undifferentiated nutritional dwarfing, and nine children (1f, 8m) with constitutional growth delay. the serum tsh, glucose, cortisol, somatotropin, prolactin, lh, and fsh were sampled periodically over 2 hours. basal t3, t4, transferrin, and somatomedin-c level ... | 1987 | 3108348 |
| elevation of serum igg levels and normalization of t4/t8 ratio after hepatitis in a patient with common variable hypogammaglobulinemia. | acute hepatitis infection developed in a 47-year-old male patient with common variable hypogammaglobulinemia as a consequence of plasma transfusion therapy. coincident with increases in serum transaminase activities indicative of acute hepatitis, serum igg levels continued to rise to 506 mg/dl. when plasma replacement therapy was stopped, a transient decline in igg level (to 371 mg/dl) was produced, followed by a sharp increase in igg to 607 mg/dl. during this period, the patient's t4/t8 ratio, ... | 1987 | 3108350 |
| autoimmune effector cells. ix. inhibition of adoptive transfer of autoimmune encephalomyelitis with a monoclonal antibody specific for interleukin 2 receptors. | this study was conducted to determine whether a monoclonal antibody (mab) specific for rat interleukin 2 receptors (il 2r) inhibits the activation of effector t cells that adoptively transfer experimental allergic encephalomyelitis (eae). mab ox 39 appears to be specific for il 2r because it binds to concanavalin a-activated, but not resting, rat lymphocytes and inhibits mitogen- and il 2-induced proliferation of rat spleen cells. moreover, this mab inhibits the in vitro activation of effector c ... | 1987 | 3108373 |
| lymphokine activation of t4+ t lymphocytes and monocytes. | the function of the t4 antigen, a marker for a differentiated t cell subset, is not well understood. our previous observation that a chemoattractant human lymphokine, lymphocyte chemoattractant factor (lcf), which selectively induces motile responses in unactivated t4+ lymphocytes, led us to investigate whether lcf could also induce t4+ cell activation. because lcf acts selectively on t4+ cells, we next determined whether the t4 antigen has a function in this lcf-induced cellular activation. a t ... | 1987 | 3108375 |
| human t cells targeted with anti-t3 cross-linked to antitumor antibody prevent tumor growth in nude mice. | human peripheral blood t cells were tested for the ability to prevent tumor growth in nude mice when targeted with anti-t3 cross-linked to antitumor antibodies. ls174t human colon adenocarcinoma cells were mixed with human pbl coated either with anti-t3 (fab) cross-linked to 315f6 (fab) (an antitumor monoclonal antibody) or with no antibody, and were injected subcutaneously into nude mice. tumor growth was totally inhibited at effector to target (e:t) ratios of 7.0:1 and 2.1:1, and was partially ... | 1987 | 3108382 |
| [purification and various properties of exonuclease from bacteriophage t4]. | exonuclease a was isolated from bacteriophage t4-infected cells of e. coli. the molecular mass of the enzyme is approximately 42,000 da, ph optimum is 7-8.5, pi is 4.05. the enzyme activity depends on mg2+, the optimal concentration of mg2+ being 1-5 mm. the enzyme splits one- and two-helical dna in the direction of 3'----5' and is a deoxyribonuclease splitting 5'-deoxynucleotides. the enzyme shows a practically equal affinity for one and two-helical dna. the km value for one- and two-helical dn ... | 1987 | 3034341 |
| pituitary response to intravenous hypothalamic releasing peptides in cynomolgus monkeys treated with contraceptive steroids. | the secretory response of the pituitary to an iv bolus dose of hypothalamic releasing peptides (hrp) was evaluated in male and cycling (cyc) or contraceptive-treated female cynomolgus monkeys. parenteral delivery of levonorgestrel and 17 beta-estradiol by intravaginal ring (cvr) was compared with oral administration (oc) of norgestrel and ethinyl estradiol in the diet. lh secretion was suppressed in the cvr group compared to that in other groups, and the response in males was greater than that i ... | 1987 | 3034957 |
| retention of enzymatic activity by n-terminal domain (1-78) t4-lysozyme: expression of synthetic dna in escherichia coli. | dna of 235 b.p. coding for n-terminal domain (1-78) t4-lysozyme was synthesized and cloned by ligating twelve synthetic fragments with a linearized plasmid puce8 followed by transformation. on expression in e. coli strain jm103 cells, colonies containing the synthetic dna were found to be lytic. on purification, clone ptly. 23-5 was found to contain polypeptide (m.w. 10,500), corresponding to n-terminal domain, its dimeric and aggregate form. it was identified by amino acid sequence analysis of ... | 1987 | 3036118 |
| effect of single amino acid changes in the region of the adenylylation site of t4 rna ligase. | preparation and analysis of a series of mutants of bacteriophage t4 rna ligase that carry single amino acid changes at or near the site of covalent reaction with atp (adenylylation) are described. the mutant proteins were constructed by site-directed mutagenesis of the gene for t4 rna ligase (g63) cloned in m13 vectors, transfer of the mutant genes into a lambda pl-containing expression plasmid, and subsequent expression in escherichia coli. the results give further evidence that lys-99 is the a ... | 1987 | 3036206 |
| rhythmicity of triiodothyronine generation by type ii thyroxine 5'-deiodinase in rat pineal is mediated by a beta-adrenergic mechanism. | type ii t4 5'-deiodinase plays an essential role in converting t4 to t3 in extrathyroidal tissues, thus allowing the full development of a cellular effect of thyroid hormone. this enzyme is one of the most important factors in regulating local action of thyroid hormone. its activity in the rat pineal was 20-30 times higher at midnight than at noon; this nocturnal rise was abolished by the beta-adrenergic blocker, propranolol. the beta-agonist isoproterenol, caused a 3-fold increase in diurnal en ... | 1987 | 3036475 |
| hypothyroidism and abnormalities in the kinetics of thyroid hormone metabolism in rats treated chronically with polychlorinated biphenyl and polybrominated biphenyl. | studies were directed at the question of whether polychlorinated biphenyl (pcb; aroclor 1254) and polybrominated biphenyl (pbb; fire master bp-6), when administered in the diets of female sprague-dawley rats over long periods of time (5-7 months) and at low dosages (0, 1, 5, 10, and 50 ppm), would depress the thyroid. by examining serum t3 and t4, kinetics of t4 metabolism, and in vivo thyroid response to exogenous tsh injections, an estimate of the degree of hypothyroidism was made, and abnorma ... | 1987 | 3036477 |
| thyroid hormone receptors in a human hepatoma cell line: multiple receptor forms on isoelectric focusing. | the nuclear thyroid hormone receptors isolated from cultured human hepatoma cells (hep g2) were characterized and compared with those from cultured human fibroblasts and rat liver. the hep g2 nuclear thyroid hormone receptors had an affinity constant (ka) of 2.1 x 10(10) m-1 and maximal binding capacity (mbc) of 21.0 fmol/100 micrograms dna for t3 in assays performed on isolated nuclei. 16% of nuclear receptors were released into the media during incubation and had the same ka. salt-extracted re ... | 1987 | 3036621 |
| use of a phage vector for rapid synthesis and cloning of single-stranded cdna. | we have developed a technique for synthesis of single stranded complementary dna (ss cdna) using specifically designed phage ssdna as vector primer. this vector (ppbs27) was constructed by introducing a poly(dt) tail adjacent to the xbai site of ptz18r, which can exist either as a plasmid in escherichia coli or as a ssdna phage. the ppbs27 phage vector is linearized with xbai using a restriction-site-directed fragment and used to anneal a mixture of poly(a) + rna for cdna synthesis by reverse tr ... | 1987 | 3036656 |
| identification of interferon-gamma as the lymphokine that mediates leukotriene b4-induced immunoregulation. | leukotriene b4 (ltb4) has been shown to modulate lymphocyte responses in both a positive and a negative way, depending on the particular cell subsets it interacts with. recent evidence also indicates that ltb4 can directly affect the production of cytokines such as interleukin 1 (il 1) or interleukin 2 (il 2) and interferon-gamma (ifn-gamma). in this report, we present evidence that human t cells pulsed with ltb4 modulate il 1 production by human monocytes by secreting ifn-gamma. in fact, we fou ... | 1987 | 3036943 |
| [immune status of patients with bronchial cancer]. | the proliferation of lymphocytes, the cell-surface markers of mononuclear cells, and the capacity of t lymphocytes to bind sheep red blood cells were studied in 61 healthy volunteers and 72 patients with small-cell carcinoma, adenocarcinoma, and squamous-cell carcinoma of the lung. the mitogen-stimulated proliferation of the lymphocytes against phytohemagglutinin (pha) was significantly reduced in patients with small-cell carcinoma. the number of t lymphocytes with t3, t4, t8, and t11 receptors ... | 1987 | 3037180 |
| sex steroid replacement in post-menopausal women: effects on thyroid hormone status. | we have measured serum thyroxine (t4), triidothyronine (t3), thyroid-stimulating hormone (tsh) free thyroxine (ft4) and thyroxine-binding globulin (tbg) levels in a total of 5 post-menopausal women who were receiving oestrogen alone (premarin, n = 19), progestogen alone (primolut-n, n = 12), a combination of oestrogen and progestogen (prempak c, n = 14) or no treatment (control group, n = 12). no differences were observed between the premarin and prempak c groups; both exhibited elevated t4 and ... | 1987 | 3037279 |
| [mechanism of reaction catalyzed by rna-ligase from bacteriophage t4]. | the dissociation constants of the complexes of rna-ligase with acceptors, donors and the adenylylated donor a(5')ppap have been determined on the basis of the inhibition of atp-pyrophosphate exchange reaction. the dissociation constants of the complexes of the enzyme with "poor" acceptors (oligouridilates) have been shown to be slightly different from those with "good" acceptors (oligoadenylates). the dependence of the reaction velocity of the formation of ligation products on the concentration ... | 1987 | 3037309 |
| expression and function of cd4 in a murine t-cell hybridoma. | the cd4 (t4) antigen was originally described as a phenotypic marker specific for helper t cells, and has recently been shown to be the receptor for the human immunodeficiency virus (hiv). functional studies using monoclonal antibodies directed at cd4 and major histocompatibility complex (mhc) class ii molecules led to the suggestion that cd4 binds to the mhc class ii molecules expressed on stimulator cells, enhancing t-cell responsiveness by increasing the avidity of t cell-stimulator cell inte ... | 1987 | 3037388 |
| interaction between the human t-cell lymphotropic virus type iiib envelope glycoprotein gp120 and the surface antigen cd4: role of carbohydrate in binding and cell fusion. | interactions between retroviruses associated with acquired immunodeficiency syndrome and their receptors on lymphocytes represent the initial steps in the process of infection and are also involved in multinucleated giant cell formation, which is one form of virus-mediated cytopathology. the exterior envelope glycoprotein of the retrovirus has been identified as gp120, and we demonstrate here that purified gp120 binds directly to cells expressing the cd4 (t4) surface antigen at a site spatially ... | 1987 | 3037551 |
| variable occurrence of the nrdb intron in the t-even phages suggests intron mobility. | the bacteriophage t4 nrdb gene, encoding nucleoside diphosphate reductase subunit b, contains a self-splicing group i intervening sequence. the nrdb intron was shown to be absent from the genomes of the closely related t-even phages t2 and t6. evidence for variable intron distribution was provided by autocatalytic 32p-guanosine 5'-triphosphate labeling of t-even rnas, dna and rna hybridization analyses, and dna sequencing studies. the results indicate the nonessential nature of the intron in nrd ... | 1987 | 3037701 |
| lymphadenopathy in macaques experimentally infected with the simian immunodeficiency virus (siv). | a t-cell tropic lentivirus of macaques the simian immunodeficiency virus (siv), has morphologic, growth, and antigenic properties that indicate that it is related to the human immunodeficiency virus (hiv), the etiologic agent of the acquired immune deficiency syndrome (aids) in humans. six juvenile macaques developed persistent lymphadenopathy (greater than 3 months in duration) after inoculation with siv. the histologic appearance of the lymph nodes was characterized by marked follicular hyperp ... | 1987 | 3037910 |
| mechanism and requirements of in vitro rna splicing of the primary transcript from the t4 bacteriophage thymidylate synthase gene. | the splicing of a procaryotic precursor rna transcribed from the t4 phage thymidylate synthase (td) gene with sp6 rna polymerase was investigated in vitro. the intron excision-cyclization reaction increased progressively to 60 degrees c. exon ligation, though barely detectable at the lower temperatures, was greatly enhanced at 60 degrees c. both reactions required mg2+. the addition of guanosine to the 5' end of an intron-exon ii intermediate via a 3',5'-phosphodiester bond was essential for the ... | 1987 | 3038175 |
| internalization of human t lymphocyte receptors. | monoclonal antibodies specific to human t lymphocyte receptors are currently being used to define the biochemical structure of these proteins as well as of functionally distinct cell subsets. since one of the antibodies (okt3) recognizing the t3 (cd3) receptor mimics vital physiological processes involved in the activation of the immune system and has been successfully used as a therapeutical agent, we investigated one of the mechanisms underlying this antibody-receptor interaction. our results ... | 1987 | 3038564 |
| decrease of rat-liver-t4-5'-deiodinase activity during chronical isoprenaline beta-action in vivo. | pure adrenergic beta-action, brought about by long-term adrenaline + regitine application in rats, was able to increase liver t4-5'-deiodinase activity for 8 h (nauman et al. 1984a). long-term isoprenaline application without alpha-blocking yielded contrary results. one of the compensatory mechanism to be probably blamed could have been the very high endogenous adrenaline-levels (porta et al. 1985). simultaneous treatment with isoprenaline and alpha-methyltyrosinemethylester did not settle the p ... | 1987 | 3038592 |
| bacteriophage t4 dna primase-helicase. characterization of oligomer synthesis by t4 61 protein alone and in conjunction with t4 41 protein. | the bacteriophage t4 41 and 61 proteins function as a primase-helicase which in vitro both unwinds double-stranded dna and synthesizes the pentaribonucleotides used to initiate dna synthesis on the lagging strand. we demonstrate that 61 protein alone possesses a weak dna template-dependent oligomer synthesizing activity, whose products differ in size and nucleotide specificity from those made by the 61 and 41 proteins together. we have previously shown that the 61 and 41 proteins make primarily ... | 1987 | 3038900 |
| bacteriophage t4 dna primase-helicase. characterization of the dna synthesis primed by t4 61 protein in the absence of t4 41 protein. | the bacteriophage t4 61/41 protein primase-helicase is part of a seven t4 protein system needed for dna synthesis in vitro. although both 41 and 61 proteins are required for the synthesis and utilization of the normal pppapc(pn)3 pentanucleotide primer, we show in the accompanying paper (hinton, d. m., and nossal, n. g. (1987) j. biol. chem. 262, 10873-10878) that high concentrations of 61 protein alone carry out a limited, template-dependent oligonucleotide synthesis with the dimers pppapc and ... | 1987 | 3038901 |
| anti-receptor antibodies designed to elicit "internal image"-bearing anti-idiotypes: a possible aids vaccine. | two obstacles hinder the development of an aids vaccine: (1) the aids virus exhibits extensive amino acid heterogeneity between isolates and (2) antibodies elicited by virus during the course of natural infection are often non-neutralizing. a vaccine designed to induce anti-idiotypic antibodies against the virus' receptor on t-cells, t4, should, in principle, overcome these obstacles. such antibody could contain an "internal image" of t4 and bind the receptor binding domain of the virus. since t ... | 1987 | 3039322 |
| endocrine responses to head and neck surgery in men. | it has been well established that surgical stress leads to profound changes in endocrine function and metabolism. however, the endocrine response varies depending upon the type and the extent of surgery. as no data were available about the endocrine changes during and following major head and neck surgery, this study was performed. plasma levels of adrenocorticotropin (acth), cortisol, thyroid-stimulating hormone (tsh), thyroxin (t4), triiodothyronine (t3), growth hormone (gh), prolactin (prl), ... | 1987 | 3039787 |
| effect of single neonatal melatonin treatment on in vitro thyroxin secretion and tsh or melatonin-modified camp level of one-month-old rats. | at the age of one month, incubation with melatonin of the thyroid glands of rats having received a single melatonin treatment at the age of three days resulted in increased thyroxine production. tsh was unable to enhance the thyroxine production of animals treated with melatonin neonatally, while its considerable increase could be observed in the case of control animals. simultaneous tsh and melatonin treatment applied in vitro at the age of one month resulted in an approximately twofold increas ... | 1987 | 3039798 |
| modulation of thyroglobulin release by dog thyroid slices. | nonbutanol-extractable 131i (nbe131i) release by dog thyroid slices in vitro has been shown previously to be primarily thyroglobulin (tg); it is stimulated by tsh. nbe131i (tg) release has therefore been considered as an in vitro model of thyroglobulin secretion and was further characterized in this work. tsh-stimulated nbe131i (tg) release, like tsh-stimulated be131i (t4, t3 and iodide) release was reproduced by forskolin, an activator of adenylate cyclase. tsh-, (bu)2camp- and forskolin-stimul ... | 1987 | 3040494 |
| surface markers of human lymphokine-activated killer cells and their precursors. analysis at the population and clonal level. | lymphokine-activated killer (lak) activity was first analyzed on pbl populations fractionated on the basis of the expression of t11 or t3 antigen. lak cell precursors were found to be present in both t11+ and t11- populations, but only in the t3- cell fraction. the generation of lak activity in highly purified t3- populations of pbl was not accompanied by expression of t3 antigen during a 5-day culture period. lak activity was next analyzed at the level of limiting dilution clonal microcultures. ... | 1987 | 3098689 |
| hyperthyroxinemia due to the coexistence of two raised affinity thyroxine-binding proteins (albumin and prealbumin) in one family. | the t4-binding proteins of a euthyroid subject with persistent hyperthyroxinemia (t4, greater than 20 micrograms/dl) were present in normal concentrations. abnormal transport of both t4 and rt3 was demonstrated by reverse flow paper electrophoresis; excess t4 was bound to albumin and prealbumin, while increased binding of rt3 was confined to prealbumin. the three t4-binding proteins in the serum of the subject were isolated by affinity chromatography and characterized. equilibrium dialysis exper ... | 1987 | 3098776 |
| dissection of defective antigen presentation by interferon-gamma-treated fibroblasts. | the capacity of interferon-gamma (ifn-gamma)-treated hla-dr expressing human dermal fibroblasts (fb) to function as antigen-presenting cells (apc) was examined. fb were cultured with 250 u/ml ifn-gamma for 4 days to induce hla-dr expression. peripheral blood monocytes (m phi), fb, or ifn-gamma-treated fb from the same donor were then cultured overnight with or without the recall antigen streptokinase streptodornase (sksd), and their capacity to stimulate autologous t4 cell dna synthesis was exam ... | 1987 | 3098840 |
| extraction of human organ-specific cancer neoantigens from cancer cells and plasma membranes with 1-butanol. | immunoprotective tumor antigens of experimental tumors are selectively extracted by 1-butanol. human organ-specific cancer neoantigens (osns) are tumor substances in cancer extracts to which patients with cancer of the same organ respond in the in vitro assay of leukocyte adherence inhibition. here we determined whether osns as measured by leukocyte adherence inhibition assay are also selectively solubilized by 2.5% (v/v) 1-butanol. butanol extracts of live tissue-cultured human cancer cells as ... | 1987 | 3100029 |
| on the albumin-dependence of measurements of free thyroxin. ii. patients with non-thyroidal illness. | we studied the relation between thyroxin-binding proteins and free thyroxin (ft4) measurements by five radioimmunoassays (ria) and an ft4 index (ft4i) in patients with non-thyroidal illness (nti). the one-step ft4 rias and the ft4i frequently failed to identify the true ft4 status (as determined by equilibrium dialysis) of nti patients. in these patients, falsely low ft4 results with one-step rias and ft4i were associated with decreasing total t3 and t4 concentrations, which, furthermore, parall ... | 1987 | 3100099 |
| iodide administration enhances thyrotrophin responsiveness to thyrotrophin-releasing hormone during fasting: evidence for normal pituitary feedback regulation. | short-term fasting in humans is associated with diminished delta tsh to trh. the purposes of the present study were to reassess basal tsh levels and trh responsiveness during fasting utilizing a sensitive radioimmunoassay (ria: sensitivity 0.3 microu/ml; normal range 0.66-2.98 microu/ml) and to determine if normal feedback regulation is maintained during the fasting state. eight control subjects (c) and six iodide-treated (i) subjects (262 mg/d) were studied in the fed state and on day 10 of fas ... | 1987 | 3100112 |
| effect of hypophysectomy on hypothalamic growth hormone-releasing factor content and release in the rat. | the possible regulation of hypothalamic gh-releasing factor (grf) by gh was studied in untreated and gh-treated hypophysectomized rats by measurement of rat hypothalamic grf content and release in vitro with a specific grf ria. two weeks after hypophysectomy, animals not receiving hormone replacement showed a marked reduction in hypothalamic grf content (to 28% of sham-operated control values; p less than 0.001). replacement therapy with t4, cortisone, and testosterone for 9 days did not correct ... | 1987 | 3100283 |
| calcium participation in thyroid function in fowl (gallus domesticus). | the effects of the calcium antagonists ethyleneglycol-bis-(beta-aminoethylether)-n,n,n',n'-tetraace tic acid (egta), cobalt chloride (cocl2), and magnesium chloride (mgcl2) on the concentrations of plasma thyroxine (t4) and triiodothyronine (t3) and on the basal and stimulated release of t4 from incubated thyroid glands have been determined in the domestic fowl. plasma t4 levels were consistently reduced 2 hr after the administration of each calcium antagonist, although only egta and cocl2 lower ... | 1987 | 3100388 |
| clonal analysis of t lymphocytes infiltrating the thyroid gland in hashimoto's thyroiditis. | t cells isolated from thyroid tissue and peripheral blood of 2 patients with hashimoto's thyroiditis were studied by a high cloning efficiency microculture technique. clonal efficiencies of 37 and 24% were obtained from thyroid-derived t cell cultures, while 40 and 90% efficiencies resulted from peripheral-blood-derived cultures. a prevalence of t4-/t8+ t cell clones were found in thyroid infiltrates. the functional analysis of the clones demonstrated significantly higher proportions of clones w ... | 1987 | 3100453 |
| cross reactivity of a monoclonal pan t-cell antibody (anti-leu 4) with prostate epithelium. | cross reactions between monoclonal t-cell antibodies and non-lymphoid tissues have rarely been reported. in this study 28 samples of prostatic tissue obtained at the time of autopsy or surgery, two samples of metastatic prostatic carcinoma and a series of other tumours were snap frozen and sections reacted with a series of monoclonal antibodies directed against the following antigens: leu 1, leu 4, t3, t8, t4, t11 and b4. reactions were detected with an indirect immunofluorescent method. in 10 o ... | 1987 | 3100824 |
| [radioimmunological and immunological follow-up of toxic goiter remission following radiotherapy and thyroidectomy]. | the paper is concerned with a multimodality approach to the assessment of clinical remission in 42 patients after radical therapy of toxic goiter. t4, t3, sigma tsh, delta tsh were determined in the thyrotropin releasing hormone stimulation test (trh-test); some immunological indices (rfcc, rfcn, rfc1, a stimulation index [si] lbtr, a migration index [mi] in the lmit and serum immunoglobulins) were also investigated. the abnormal trh-test was revealed in 2/3 of the patients (the hypo-trh-test in ... | 1987 | 3100903 |
| immunohistological analysis of the lymphoid infiltrate in cutaneous malignant melanomas. | the immunological phenotypes of the lymphoid cells in 39 cutaneous malignant melanomas have been investigated by staining cryostat sections with a panel of 20 monoclonal antibodies against lymphoid cells and their subsets. staining was performed by the alkaline phosphatase: anti-alkaline phosphatase (apaap) method in which the substrate label (red) is easily distinguishable from melanin. the lymphoid infiltrates had an essentially identical composition in all cases, consisting of t-lymphocytes a ... | 1987 | 3101284 |
| the pituitary tsh response to trh is inversely related to the plasma tsh concentration and directly related to the pituitary tsh content during hypothyroidism in the rat. | we studied the effects of degree and duration of hypothyroidism on the pituitary tsh concentration and the pituitary tsh secretory response to trh. varying degrees of hypothyroidism were achieved in thyroparathyroidectomized rats (thyrex) by continuous sc infusion of t3 (0.2, 0.3, 0.4, or 0.5 microgram/100 g x day) or t4 (0.6, 1.2, or 1.8 microgram/100 g x day). while t3 was more potent than t4, both resulted in a dose-dependent suppression of the post-thyroidectomy rise in tsh. after 7 or 14 da ... | 1987 | 3101338 |
| serum concentrations of thyrotropin, thyroxine, triiodothyronine and thyroxine binding globulin in female endurance runners and joggers. | the effects of endurance training and season on the function of the anterior pituitary-thyroid axis were studied in 18 female runners and their 12 controls, and in 13 joggers and their 11 controls in northern finland, with a large seasonal difference in environmental factors. the serum concentrations of thyrotropin (tsh), thyroxine (t4), free thyroxine (ft4), triiodothyronine (t3), thyroxine binding globulin (tbg) and oestradiol (e2) were measured during one menstrual cycle in the light training ... | 1987 | 3101339 |
| natural history of acquired immunodeficiency syndrome in hemophilic patients. | during the 5-year period from 1981 to 1985, we have observed 8 cases of acquired immunodeficiency syndrome (aids) among our 85 patients with hemophilia a. thus, the prevalence of aids with hemophilia a is 9.4% in our patient population. by utilizing stored serum or plasma samples dating back to 1978, antibody against htlv-iii was detected in all 8 cases with aids. based on the time interval from the appearance of antibody to htlv-iii to the diagnosis of aids in these patients, the incubation per ... | 1987 | 3101490 |
| hypothyroxinemia in cardiac arrest. | thyroid function was evaluated in cardiac arrest (ca), a condition associated with marked activation of the pituitary-adrenal axis. blood samples were obtained in 24 patients immediately after diagnosis of ca and again ten minutes later. samples were also obtained from 22 patients admitted consecutively to the intensive care unit (icu). abnormalities of thyroid indexes among patients on the icu who had not experienced ca were low triiodothyronine (t3) in 45%, low thyroxine (t4) in 32%, low free ... | 1987 | 3101625 |
| assessment of sensitive thyrotropin assays for an expanded role in thyroid function testing: proposed criteria for analytic performance and clinical utility. | assays capable of detecting subnormal serum tsh concentrations can permit the prediction of response to trh and the detection of hyperthyroidism. five performance criteria for evaluating the eligibility of tsh assays for these new roles are proposed. criterion 1 requires a less than 1% overlap between the variation of the lower normal value and the assay detection limit. criteria 2 and 3 require subnormal basal tsh values in 95% of patients with subnormal trh response, and detectable basal tsh v ... | 1987 | 3102536 |
| accessory cell independent proliferation of human t4 cells stimulated by immobilized monoclonal antibodies to cd3. | the capacity of the monoclonal antibodies (mab) 64.1 and okt3 directed at cd3 molecules to induce t4 cell proliferation and interleukin 2 (il 2) production was examined. each was tested in soluble form or was immobilized by adhering it to the wells of plastic microtiter wells. soluble anti-cd3 did not induce proliferation of accessory cell (ac)-depleted t4 cells. in contrast, immobilized anti-cd3 induced t4 cell il 2 production and proliferation in the complete absence of ac. when t4 cells were ... | 1987 | 3102594 |
| interference of dimethyl alpha-(dimethoxyphosphinyl) p-chlorobenzyl phosphate (sr-202) in thyroid hormone metabolism: evidence of inhibition of monodeiodination. | sr-202 is a non-iodinated potential lipid-altering agent. when administered (100 mg) three times per day for 3 days to six euthyroid subjects it was associated with a 30 +/- 3% (mean +/- s.e.m.) fall in 3,3',5-triiodothyronine (t3) (p less than 0.001), a reciprocal 104 +/- 14% rise in 3,3',5'-tri-iodothyronine (reverse t3, rt3) (p less than 0.01), and a 37 +/- 7% rise in thyroxine (t4) (p less than 0.001). basal and trh-stimulated tsh did not change. these results suggested that sr-202 was actin ... | 1987 | 3102663 |
| evidence for neoplastic cell differentiation in mediastinal t lymphoblastic lymphoma. | immunostaining of frozen sections from a mediastinal t lymphoblastic lymphoma (t-ll) revealed the existence of two neoplastic cell populations characterized by different degrees of maturation. several large nodules of 3a1+/t11+/t9+ t6-/t4-/t8-/t3- lymphoid cells, resembling normal early thymocytes, were surrounded by 3a1+/t11+/t9+/t6+/t4+/t8+/t3- cells resembling normal cortical thymocytes. the junctional area between early and cortical lymphocytes was occupied by numerous leu-m3+/pam-1+/dr+ ret ... | 1987 | 3103325 |
| susceptibility to infection by the human immunodeficiency virus (hiv) correlates with t4 expression in a parental monocytoid cell line and its subclones. | the monocytoid tumor cell line u-937 and five derived subclones were infected with the htlv-iiib isolate of the human immunodeficiency virus (hiv). susceptibility to infection and sensitivity to the cytopathic effects correlated with the expression of the t4 antigen on the cell surface. on the basis of these characteristics the lines could be divided into three groups. less than 10% t4 positive cells were present in the parental line and clone 4; hence productive infection could only be establis ... | 1987 | 3103330 |
| t-cell prolymphocytic leukemia (t-pll) with unique surface phenotype. | leukemic cells of a 19 year old patient with prolymphocytic leukemia of t-cell type (t-pll) were characterized by surface markers and immunologic functions. phenotypic analysis using a large panel of monoclonal antibodies corresponding to the clusters (cd) of differentiation antigens established on the leukocyte typing workshops i and ii revealed a unique t-cell phenotype not yet reported in the literature: cd1 (t6)-, cd2 (t11)+, cd3 (t3)+, cd4 (t4)-, cd5 (t1)-, cd6 (t411)+, cd7 (leu9)+, cd8 (t8 ... | 1987 | 3103723 |
| characterization of the t3+t4+t8+ thymocyte intermediate in vitro. | sensitive two-color fluorescence staining and cell-sorting techniques were used to isolate a t4+t8+ thymocyte subpopulation with high t3 density from human thymocyte cultures. previously, this population was shown to give rise to both t4+t8- and t8+t4- thymocytes. in the present study, this t3+t4+t8 population was shown to be functionally as well as phenotypically distinct from either t8+t4- cells or t4+t8- cells present in the same culture. the t3+t4+t8+ cell had intermediate cytotoxic capaciti ... | 1987 | 3102076 |
| involvement of the cd4 molecule in a post-activation event on t cell proliferation. | a monoclonal antibody (mab) directed to a human leukocyte 55-kda cell surface molecule with identical cellular distribution and biochemical properties to the cd4 was able to inhibit t cell proliferation induced either in a mixed lymphocyte culture or by activation with mab anti-cd3, anti-cd2 or phytohemagglutinin. the inhibitory effect of anti-cd4 was observed in the absence of monocytes and was directly exerted on t4+ cells. this effect on cellular proliferation appears to be due to an inhibiti ... | 1987 | 3104060 |
| [studies on cytosol thyroid hormone binding proteins in the rat liver: part iii. partial purification and binding characteristics of thyroxine-binding protein in hypothyroid rat liver cytosol and serum]. | cytosolic thyroxine-binding protein (ct4bp) was partially purified from rat liver cytosol obtained 10 days after thyroidectomy using sephadex g-200 gel filtration, and its binding characteristics were analyzed in displacement experiments using a charcoal binding method to separate bound and free hormones. serum t4-binding proteins were also partially purified, and their binding characteristics were similarly determined. sephadex g-200 gel filtration of liver cytosol from thyroidectomized rats re ... | 1987 | 3104103 |
| abnormalities in pituitary thyroid axis function tests in patients with paroxysmal supraventricular arrhythmias. | the study was carried out on 60 consecutive patients (23 males and 37 females) aged between 20 and 83 years (means +/- sd, 40.7 +/- 16) who arrived at our cardiologic unit with paroxysmal supraventricular arrhythmias (psva) including junctional paroxysmal tachycardia (n = 32), atrial fibrillation (n = 13), atrial flutter (n = 1), premature beats (n = 13) and with no obvious cardiovascular causes. serum thyroxine and triiodothyronine were normal in all patients and thyroid scintiscan revealed nor ... | 1987 | 3104185 |
| feasibility of non-surgical definitive management of anal canal carcinoma. | the management of anal canal carcinoma has evolved over the last decade, with the combination of radiotherapy (rt) and chemotherapy (ct) offering a definitive alternative to surgery or rt alone. twenty-two patients with basaloid/cloacogenic/squamous cell carcinoma of the anal (13 with t3-t4 lesions), were treated with synchronous rt (30.00 to 45.00 gy in 3 1/2 to 5 weeks) and ct x 2 courses (mitomycin c 15 mg/m2 i.v. bolus + 96 hours 5fu infusion). this regimen differed from the conventional nig ... | 1987 | 3104242 |
| il 1 expression in a clone of human t cells. | three human t cell clones, all of which are t3+, t4+, t8-, and t11+, were examined for il 1 production. two clones were found to express readily detectable, membrane-bound il 1 activity upon stimulation with okt3 antibody, ril 2, and pma. northern blot analysis of rna from one of the clones shows that cells can be induced to express the genes for both il 1 alpha and il 1 beta. furthermore, the pattern of expression in response to different stimuli suggests that the genes for il 1 alpha and il 1 ... | 1987 | 3104451 |
| t cell surface markers expression by immature human thymocytes in in vitro culture: role of ia+ accessory cells. | previous studies have indicated that the human thymus is composed of several discrete compartments. cortical thymocytes are reactive with the monoclonal antibody anti-t6, whereas most medullary cells, unreactive with anti-t6, stain brightly with anti-t3 antibody, which defines mature t cell populations. by using an indirect immune rosette method, we isolated the minor thymocyte population (1 to 2% of all thymocytes) lacking both t3 and t6 but expressing t11 antigens. these cells could be maintai ... | 1987 | 3104465 |
| heterogeneous expression of leu3/t4 antigen on cord t cells. | 1987 | 3104514 | |
| analysis of human prenatal and postnatal thymocytes with the anti-tac monoclonal antibody and recombinant interleukin-2. | the expression of the interleukin-2 (il-2) receptor on the cell surface membrane of human prenatal and postnatal thymocytes has been studied by flow cytometry. the in vitro responsiveness of these thymocytes to human recombinant il-2 (ril-2) was also studied. prenatal and postnatal thymocytes contained very few tac positive cells. the different t cell antigens t6, t3, t4, t8, t9, t10, 3a1 and t11 were expressed by the first trimester of fetal development. recombinant il-2 alone was able to induc ... | 1987 | 3109082 |
| the effects of melatonin and hypothyroidism on estradiol and gonadotropin levels in female syrian hamsters. | since melatonin injections administered near the end of the daily photoperiod influence both gonadal and thyroid hormones in the female hamster, the present study was designed to compare the effects of melatonin and hypothyroidism on the reproductive system and to determine whether thyroid status influenced the action of melatonin on the regulation of the hormones of reproduction. the effects of daily melatonin injections were determined in control hamsters, in hamsters rendered hypothyroid with ... | 1987 | 3109508 |
| selecting initial therapy for bladder cancer. | progress has been made at both ends of the spectrum of bladder cancer. the introduction and increasing use of effective intravesical agents for both treatment and prophylaxis of tumors limited to the mucosa or lamina propria has reduced the incidence and frequency of subsequent tumors. at the other end of the spectrum--patients with locally extensive bladder cancer--neoadjuvant or initial chemotherapy is producing complete and partial responses. hopefully this will translate into an improvement ... | 1987 | 3109725 |
| subcutaneous recombinant gamma interferon in cancer patients: toxicity, pharmacokinetics, and immunomodulatory effects. | recombinant gamma interferon (r gamma-ifn) was administered s.c. daily to 26 patients with advanced cancer. patients were assigned to one of six doses: 0.5, 1, 2, 4, 6, or 8 million units (mu)/m2 per d. the major toxicities were an influenza-like syndrome and fever, seen in all patients. dose limiting toxicity occurred in 4 of 4 patients treated at 8 mu/m2. one patient with nodular poorly differentiated lymphocytic lymphoma had a mixed response, and two patients with renal cell cancer have had s ... | 1987 | 3109737 |
| decrease of free thyroxin in serum of lactating women. | we measured thyroid hormones and thyroxin-binding proteins in serum from 62 normal lactating and 52 nonlactating women at three months postpartum, and compared these values with those for 42 nonpregnant control women of similar age. mean thyroxin concentrations in the lactating and nonlactating women were significantly (p less than 0.001) lower than that of the nonpregnant controls, but there was no significant difference (p greater than 0.2) in triiodothyronine concentration among these three g ... | 1987 | 3109780 |
| definitive combined modality therapy of carcinoma of the anus. a report of 30 cases including results of salvage therapy in patients with residual disease. | thirty patients with epidermoid carcinoma of the anus, ranging in age from 40 to 89 years, were treated with combined chemotherapy (ct) and radiation therapy (rt) in lieu of abdominoperineal resection. two courses of 5-fu (1000 mg/m2/day x four days) by continuous infusion and mitomycin-c (10-15 mg/m2 iv bolus on day 1 of each course) were given 3 to 4 weeks apart simultaneously, with whole pelvis rt to 4140 to 4500 cgy. twenty-one of 28 patients had t3-t4 primaries and ten had positive nodes (n ... | 1987 | 3109860 |
| the effect of free radicals on hepatic 5'-monodeiodination of thyroxine and 3,3',5'-triiodothyronine. | free radicals have been implicated in many pathological processes, including ischemia, inflammation, and malignancy. since a reduction in extrathyroidal outer ring monodeiodination of t4 and rt3 occurs in virtually all systemic illnesses, we have studied the effect of free radicals on iodothyronine (t4 and rt3) 5'-monodeiodinating activity (ma) of liver tissue in vitro. rat liver microsomes or homogenate were preincubated in tris buffer for 30 min with a free radical-generating system (frgs) and ... | 1987 | 3109883 |
| lower serum free thyroxine (t4) levels in painless thyroiditis compared with graves' disease despite similar serum total t4 levels. | serum total t4 (t4), total t3 (t3), free t4 (ft4), free t3 (ft3), and t4-binding globulin concentrations and t3 resin uptake values were measured in 17 women with thyrotoxicosis due to painless thyroiditis (pt) and compared with the same parameters in 17 women with thyrotoxicosis due to graves' disease (gd) with similar serum t4 levels. the mean serum t3 resin uptake value and t3, ft4, and ft3 concentrations in the pt patients were significantly lower than those in the gd patients. the mean seru ... | 1987 | 3110204 |
| a four generation study of familial dysalbuminemic hyperthyroxinemia: diagnosis in the presence of an acquired excess of thyroxine-binding globulin. | we have studied the largest kindred with familial dysalbuminemic hyperthyroxinemia (fdh) thus far reported, comprising thirty-three blood relations in four generations and three of their spouses. our objective was to complement previous evidence concerning the precise mode of inheritance of fdh and to detect any other features of the disorder that had not yet been noted. among the thirty three, there were thirteen patients with fdh, eight males and five females, in all of whom the abnormality ap ... | 1987 | 3110251 |
| hypothalamic secretion of thyrotropin releasing hormone declines in aging rats. | young and aged male rats were used in experiments to investigate a possible decline in hypothalamic secretion of thyrotropin releasing hormone (trh) to the anterior pituitary of aging mammals. we observed a 66% decrease in basal trh release by incubated rat hypothalami with aging. thyroid hormone-responsive hepatic alpha-glycerophosphate dehydrogenase (gpd) and malic enzyme (me) levels in aged rats did not differ from 5-month-old controls in spite of a significant fall in serum thyroxine (t4) le ... | 1987 | 3110255 |
| cross-linking of t3 (cd3) with t4 (cd4) enhances the proliferation of resting t lymphocytes. | monoclonal antibodies reactive with defined t lymphocyte surface antigens were covalently coupled to protein a-sepharose beads using the bifunctional imidoester, dimethyl pimelimidate. sepharose-immobilized antibody reactive with t3 induced the proliferation of resting t lymphocytes in the presence of either recombinant interleukin 2 or phorbol myristate acetate. when monoclonal antibodies reactive with t3 and t4 were coupled to the same sepharose bead (hereafter designated sepharose (t3:t4)), p ... | 1987 | 3110278 |
| changes in thyroid hormones and thyroxine glucuronidation in hamsters compared with rats following treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin. | in rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) and related compounds, serum thyroxine (t4) is depressed. since hamsters are relatively insensitive to tcdd-induced lethality, the effects of tcdd on several parameters of thyroid status were measured in hamsters as a comparison with the more sensitive rat. at 7 days after ip injection of tcdd, there was a dose-dependent increase in serum 3,5,3'-triiodothyronine (t3) and t4 in hamsters to a maximum level 200% of control; the ed50 was ... | 1987 | 3111013 |
| clinical and immunological restoration in patients with aids after marrow transplantation, using lymphocyte transfusions from the marrow donor. | the diagnosis of transfusion-associated acquired immunodeficiency syndrome (aids) was made in 2 patients who developed delayed opportunistic infections and severe cytopenias--56 months for the former (patient 1) and 22 months for the latter, (patient 2) following bone marrow transplantation (bmt) for aplastic anemia. in the third case, grafting for acute leukemia (patient 3) (hiv) infection was probably responsible for the failure of hematological and immunological reconstitution 8 months after ... | 1987 | 3111037 |
| [results of the gnrh-trh and arginine-gnrh-trh test in females with hyperprolactinemic galactorrhea]. | in 7 patients aged from 21 to 33 years (average age 24.6 years) with hyperprolactinemic galactorrhea a stimulation test with gnrh-trh or arginine-gnrh-trh was performed. the serum levels of lh, fsh, prl, hgh, tsh and total thyroxine (t4) were determined by ria, the thyroxine binding capacity (tbc) by radio agent assay. the free thyroxine index (ft4-i) was calculated. an adenoma of the pituitary gland was diagnosed in 3 patients by x-ray of sella turcica. in all patients there were disturbances o ... | 1987 | 3111128 |
| [gnrh-trh and arg-gnrh-trh test in females with normoprolactinemic galactorrhea]. | in 8 patients aged from 17 to 48 years (average age 32,6 years) with normoprolactinemic galactorrhea a stimulation test with gnrh-trh or arginine-gnrh-trh was performed. the basal and stimulated serum levels of lh, fsh, prl, tsh, hgh and total thyroxine (t4), the thyroxine binding capacity (tbc) and the free thyroxine index (ft4-i) were determined. -in a few cases there were disturbances of both the basal and the stimulated serum levels of the hormones. -both the menstrual disorders which were s ... | 1987 | 3111129 |
| systemic effects of 1,25-dihydroxyvitamin d3 on the pituitary-hypothalamic axis in rats. | treatment of rats with 1,25-dihydroxyvitamin d3 (1,25(oh)2d3) 0.05 microgram/kg per day for three days was without any effect on serum t3, t4 or tsh concentrations, whereas serum prl increased (20.6 +/- 3.8 to 76.2 +/- 19.1 micrograms/l, mean +/- sem, n = 7-8; p less than 0.01). increased hypothalamic trh levels (24.3 +/- 3.9 to 45.7 +/- 7.8 pmol/g wet weight; p less than 0.01) may indicate an effect of 1,25(oh)2d3 on hypothalamic trh homeostasis. this effect could probably be due to an indirect ... | 1987 | 3111137 |
| diagnostic and prognostic value of monoclonal antibodies in immunophenotyping of t cell lymphomas. | 6 cases of post-thymic t cell lymphoma (ptcl) and 4 cases of t lymphoblastic lymphoma were investigated with a panel of 21 monoclonal antibodies (moab). the ptcl group was composed of 2 by 2 cases of lymphoepithelioid cell lymphoma, t zone lymphoma and t immunoblastic lymphoma. in lymphoepithelioid cell lymphoma, the cell proliferating activity was low, and a malignant clone was not identifiable by the methods used. in the cases of t zone lymphoma and t immunoblastic lymphoma, malignant populati ... | 1987 | 3111143 |
| trh-induced tsh and prolactin responses in the elderly. | since there are divergencies in the thyrotropin (tsh) response to thyrotropin-releasing hormone (trh) in old age, and since a hypothalamopituitary dysfunction has been suggested in the elderly, we have studied the thyroid function and the trh responsiveness of tsh and prolactin (prl) in 56 euthyroid patients over 70 years old, grouped according to age (70-79, 80-89, 90 or more years) and sex. results were compared to those of 15 postmenopausal women and 11 men. in the elderly patients there was ... | 1987 | 3111192 |
| activation of autoreactive cytolytic t lymphocyte clone against human melanoma by anti-t3 monoclonal antibody and autologous accessory cells. | a cytotoxic t-lymphocyte (ctl) clone tc1.8 was derived in a limiting dilution culture from a single cell that was derived from melanoma-involved lymph node lymphocytes activated in in vitro coculture against the autologous melanoma cells (vip). the clone tc1.8 (t3+, t8+, t4-, and leu7-) expressed restricted cytolytic activity against only the autologous target vip. as it aged in continuous culture containing interleukin 2, tc1.8 lost cytolytic activity. the cytolytic function could be restored, ... | 1987 | 3111724 |
| concentrations of iodothyronines in serum of patients with chronic renal failure and other nonthyroidal illnesses: role of free fatty acids. | the mean concentration of free thyroxin (ft4) in serum, as determined by direct equilibrium dialysis, was decreased in patients with chronic renal failure (crf) and increased in patients with various other nonthyroidal illnesses (nti). the mean concentration of dialyzable free triiodothyronine (ft3) in serum was equally low in both groups of patients. patients with crf of various etiology but a similar degree of renal failure as estimated from serum creatinine assay had very similar concentratio ... | 1987 | 3111749 |
| theophylline treatment in the neonate with apnea: effect on growth hormone, thyroid hormone and trh induced tsh secretion. | caffeine has been shown to markedly alter growth hormone (gh), thyroid stimulating hormone (tsh), and thyroid hormones in animal studies. similar studies in the human are lacking. to determine the effect of theophylline treatment on endocrine function in neonates with apnea, 10 infants were studied prospectively pretreatment, immediately following therapeutic blood levels of theophylline, at 2, 4, and 6 weeks thereafter and finally 2 weeks after discontinuation of theophylline. t4, free t4, t3, ... | 1987 | 3111805 |
| changes in thyroid function tests and sex hormone binding globulin associated with treatment by gonadotropin. | increasing levels of e2 in gonadotropin-treated women stimulated hepatic synthesis of shbg and tbg, and consequently increases in t4 concentration. nevertheless, unchanged fti and tsh suggested that a euthyroid state was maintained. the temporal patterns for the rise in serum concentrations of tbg and shbg during gonadotropin therapy suggest that the synthesis of these proteins by the liver has different sensitivities to e2. | 1987 | 3111894 |
| [studies on the behavioral development of rats treated neonatally with l-thyroxine]. | the effect of l-thyroxine administration from 2 to 10 days of age (neo-t4) on the behavioral development was studied. serum t4 levels in neo-t4 rats showed a marked and dose-related decrease at 15 days of age and a less marked decrease at 62 days of age. the locomotor activity of the neo-t4 rats was higher at 13 approximately 19 days of age and tended to be lower at 62 days of age than in the controls. at 17 days of age, apomorphine (0.1 mg/kg, s.c.) induced a marked increase in the frequency of ... | 1987 | 3111957 |