Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| alkali-labile sites in sperm cells from sus and ovis species. | constitutive alkali-labile sites (alss) have been investigated using a protocol of dna breakage detection-fluorescence in situ hybridization (dbd-fish) in sperm cells from sus domesticus (pig), ovis gmelini musimon (mouflon) and ovis aries (sheep). the results were compared with those obtained using leucocytes from the same species. whole comparative genomic hybridization (w-cgh) showed that most of the constitutive alss in somatic and germ line cells in all species examined were constrained to ... | 2008 | 17651406 |
| a review of viral diseases of the european wild boar: effects of population dynamics and reservoir rôle. | there has been a worldwide increase in the number and geographical spread of wild boar populations in recent decades leading to an increase in both the circulation of disease agents and greater contact with domestic animals and humans. diseases affect the population dynamics of wildlife but the effects of most viral diseases on the european wild boar are largely unknown. many viral diseases present in domestic pig populations are also present in wild boars where they can provide a disease reserv ... | 2008 | 17420149 |
| laser-induced thermotherapy for lung tissue--evaluation of two different internally cooled application systems for clinical use. | thermal ablation techniques like radiofrequency or laser-induced thermotherapy (litt) are increasingly used to treat tumors of parenchymatous organs. minimal access, parenchymal preservation, and a low complication rate render them suitable for pulmonary tumors as well. their successful clinical application depends on the induction of sufficiently large lesions and a knowledge of the energy parameters required for complete thermal ablation. the aim of this study was to establish a dose-response ... | 2008 | 17599236 |
| histopathological classification of systemic mycobacterium avium complex infections in slaughtered domestic pigs. | the aim of this study was to classify the histopathological features of pigs infected with mycobacterium avium complex (mac). we used slaughtered pig organs systemically infected with mac. the results showed granulomatous lesions which were observed predominantly in the digestive organs and regional lymph nodes rather than respiratory organs. the histological picture showed a wide range of granulomatous stages from exudative to fibrotic reactions to the mac infection. eosinophils and giant cells ... | 2008 | 17629560 |
| intravenous tezosentan improves gas exchange and hemodynamics in acute lung injury secondary to meconium aspiration. | meconium aspiration induces acute lung injury (ali) and subsequent pulmonary arterial hypertension (pah) which may lead to right ventricular failure. increase of endothelin-1, thromboxane-a, and phosphodiesterases are discussed molecular mechanisms. we investigated the intrapulmonary and hemodynamic effects of the intravenous dual endothelin a and b receptor blocker tezosentan and inhalational iloprost in a model of ali due to meconium aspiration. | 2008 | 17898997 |
| cryopreservation of the germplasm of animals used in biological and medical research: importance, impact, status, and future directions. | molecular genetics and developmental biology have created thousands of new strains of laboratory animals, including rodents, drosophila, and zebrafish. this process will accelerate. a decreasing fraction can be maintained as breeding colonies; hence, the others will be lost irretrievably unless their germplasm can be cryopreserved. because of the increasingly critical role of cryopreservation, and because of wide differences in the success with which various forms of germplasm can be cryopreserv ... | 2008 | 17901073 |
| photoperiod and luteinizing hormone secretion in domestic and wild pigs. | during seasonal anoestrus (long-days), oestradiol can effectively inhibit the pulsatile secretion of luteinizing hormone (lh) in sheep. the aim of our trial was to determine whether the same regulatory mechanism exists in the pig. altogether, 20 ovariectomized and oestradiol-implanted gilts (16 domestic pigs, 4 european wild boars) were randomly allocated to two treatment groups. the first group was kept under a short-day light-dark cycle of 8l:16d, and the second group under a long-day light re ... | 2008 | 17250979 |
| intraindividual comparison of image quality in mr urography at 1.5 and 3 tesla in an animal model. | experimental evaluation of image quality of the upper urinary tract in mr urography (mru) at 1.5 and 3 tesla in a porcine model. | 2008 | 19238642 |
| serial noninvasive in vivo positron emission tomographic tracking of percutaneously intramyocardially injected autologous porcine mesenchymal stem cells modified for transgene reporter gene expression. | porcine bone marrow-derived mesenchymal stem cells (mscs) were stably transfected with a lentiviral vector for transgene expression of the trifusion protein renilla luciferase, red fluorescent protein and herpes simplex truncated thymidine kinase (lv-rl-rfp-ttk; positron emission tomography [pet] reporter gene) for in vivo noninvasive tracking of the intramyocardially delivered msc fate. | 2008 | 19808526 |
| minimal interruption of cardiopulmonary resuscitation for a single shock as mandated by automated external defibrillations does not compromise outcomes in a porcine model of cardiac arrest and resuscitation. | current automated external defibrillations require interruptions in chest compressions to avoid artifacts during electrocardiographic analyses and to minimize the risk of accidental delivery of an electric shock to the rescuer. the earlier three-shock algorithm, with prolonged interruptions of chest compressions, compromised outcomes and increased severity of postresuscitation myocardial dysfunction. in the present study, we investigated the effect of timing of minimal automated external defibri ... | 2008 | 18824916 |
| endothelin-1 is not predictive of ventricular ectopy or ventricular fibrillation during acute myocardial ischemia. | endothelin(et)-1 (et-1) increases after myocardial infarction and may have effects on myocardial function. et-1 has also been shown to affect the action potential (ap) which may be arrhythmogenic and predispose to ventricular fibrillation (vf). the effects of et-2 and et-3 are uncertain. we hypothesized that the ets increase during acute ischemia and that plasma levels are predictive of ischemically induced vf. thirty-four domestic swine underwent balloon occlusion of the proximal lad coronary a ... | 2008 | 18844577 |
| spatial distribution of taenia solium porcine cysticercosis within a rural area of mexico. | cysticercosis is caused by taenia solium, a parasitic disease that affects humans and rurally bred pigs in developing countries. the cysticercus may localize in the central nervous system of the human, causing neurocysticercosis, the most severe and frequent form of the disease. there appears to be an association between the prevalence of porcine cysticercosis and domestic pigs that wander freely and have access to human feces. in order to assess whether the risk of cysticercosis infection is cl ... | 2008 | 18846230 |
| restenosis is not associated with stent length in a pig model of coronary stent implantation. | the aim of this study was to determine if stent length is by itself a risk factor for intimal proliferation and restenosis. long lesions represent an independent risk factor for restenosis after coronary stent implantation. a longer stented segment might result in a higher probability of restenosis. | 2008 | 18810722 |
| the effect of continuous release of recombinant human epidermal growth factor (rh-egf) in chitosan film on full thickness excisional porcine wounds. | the purpose of this article is to evaluate the effect of continuously released recombinant human epidermal growth factor (rh-egf) in chitosan film in full thickness porcine wounds. a total of 10 domestic pigs (yorkshire species) weighing 18 to 22 kg between the ages of 50 to 60 days were used. the wounds were divided into 3 groups and treated selectively with rh-egf in chitosan film (egf 20 ug/wound/d), chitosan film without rh-egf, or remained as the control group. one hundred percent healing t ... | 2008 | 18812721 |
| vocal mother-offspring communication in guinea pigs: females adjust maternal responsiveness to litter size. | abstract: | 2008 | 18783602 |
| the proinflammatory cytokine response following resuscitation in the swine model depends on the method of ventricular fibrillation induction. | a systemic inflammatory response has been reported following resuscitation from cardiac arrest. the purpose of this study was to compare the magnitude of the tumor necrosis factor-alpha (tnf-alpha) response in two different swine models of ventricular fibrillation (vf) arrest. | 2008 | 18785945 |
| sleep-induced hypotension precipitates severe myocardial ischemia. | epidemiologic studies have shown a high frequency of major cardiac events at night in patients with coronary artery disease. this has been attributed to the sympathetic surges accompanying rapid eye movement (rem) sleep; the role of non-rem sleep, which comprises 80% of total sleep duration, has been largely neglected. accordingly, we evaluated the effect of non-rem sleep on contractile function in a region of the left ventricular wall supplied by a flow-limiting coronary stenosis. | 2008 | 18788646 |
| isolation and cloning of two variant papillomaviruses from domestic pigs: sus scrofa papillomaviruses type 1 variants a and b. | the healthy skin of two female domestic pigs (sus scrofa domestica) was sampled with cotton-tipped swabs. total genomic dna was extracted from the samples and subjected to pcr with degenerate papillomavirus (pv)-specific primers. similarity searches performed with blastn showed that partial e1 and l1 sequences of two novel pvs were amplified. subsequently, the complete genomes of these sus scrofa papillomaviruses (sspvs) were amplified by long-template pcr, cloned and sequenced using a transposo ... | 2008 | 18796716 |
| intravenous tezosentan and vardenafil attenuate acute hypoxic pulmonary hypertension. | excessive hypoxic pulmonary hypertension imposes right ventricular strain by increasing afterload that may lead to right heart failure and death. increased phosphodiesterase activity, as well as increased levels of endothelin-1, has been discussed as molecular mechanisms. we investigated the hemodynamic and intrapulmonary effects of the intravenous dual endothelin a and b receptor blocker tezosentan, and of the phosphodiesterase-5 (pde-5) antagonist vardenafil in a pig model of acute normobaric ... | 2008 | 18800959 |
| finite element representation of bone substitute remodelling in the jaw bone. | the finite element (fe) method was originally developed on a physical basis for the computation of structure-mechanical problems. meanwhile, it has been widely applied to medical issues. this study sought a suitable method to build a fe model for remodelling processes in osseous defects supplemented with bone substitute material. for this purpose, the second right premolars were extracted from four pig mandibles (sus scrofa domesticus) and the extraction alveoli were provided with synthetic bone ... | 2008 | 18803527 |
| development of a swine model of secondary liver tumor from a genetically induced swine fibroblast cell line. | metastatic disease is the most common liver tumor. although alternative therapies have been developed for non-surgical candidates, those therapies lacked ideal testing prior to clinical application because of a paucity of large animal models. the purpose of the present study was to develop a model for secondary liver tumor in a large animal. | 2008 | 18773055 |
| development of two trichoplusia ni larvae-derived elisas for the detection of antibodies against replicase and capsid proteins of porcine circovirus type 2 in domestic pigs. | the main aim of the present study was to describe new methods for the identification of antibodies against the pcv2 capsid (cap) and replicase (rep) proteins in pig sera. specifically, two new indirect enzyme-linked immunosorbent assays (elisa) were developed based on recombinant pcv2 cap (rcap) and rep/rep' (rrep) proteins expressed in baculovirus and produced in trichoplusia ni insect larvae. both assays were validated by testing serum samples in a longitudinal study of 107 animals with differ ... | 2008 | 18773923 |
| natural orifice translumenal endoscopic surgery (notes) renal cryoablation in a porcine model. | to present our laboratory experience with natural orifice translumenal endoscopic surgery (notes) renal cryoablation. | 2008 | 18778358 |
| [molecular identification of heparin from pigs by allele-specific pcr (as-pcr) and amplification refractory mutation system (arms)]. | the aim of this study is to develop a convenient and effective method for the identification of heparin from pigs (include sus scrofa domestica brisson and sus scrofa riukiuanus). based on sequences of d-loop region of pigs and the other animals, two pairs of highly specific primers were designed for distinguishing heparin of pigs from other animals. the primers were employed to amplify d-loop region of dna templates extracted from pig and seven other animal species that amounted to 49 samples. ... | 2008 | 18717344 |
| human dispersal of trichinella spiralis in domesticated pigs. | to investigate the human impact on the evolutionary ecology of animal pathogens, we compared genetic diversity of severe foodborne parasites contracted by eating infected pork or wild game. in particular, we characterized trichinella spp. from twenty-eight countries and four continents by genotyping nine microsatellite loci and sequencing one-fifth of the mitochondrial genome. all specimens of trichinella spiralis, a swine parasite that can infect many species of wildlife, were remarkably unifor ... | 2008 | 18718558 |
| notes: a review of the technical problems encountered and their solutions. | natural orifice translumenal endoscopic surgery (notes) is currently investigated and developed worldwide. in the past few years, multiple groups have confronted this challenge. many technical problems are encountered in this technique due to the currently available tools for this approach. some of the unique technical problems in notes include: blindly performed primary incisions; uncontrolled pneumoperitoneal pressure; no support for the endoscope in the abdominal cavity; inadequate vision; in ... | 2008 | 18721009 |
| evaluation of unmeshed and 1:1 meshed alloderm bolsters for stapled rectal anastomoses in a porcine model. | the major morbidity of colorectal anastomoses is leaks. the concept of staple-line reinforcement is a growing area of interest. in this study, we evaluated the feasibility and effect of utilizing alloderm to bolster end-to-end stapled rectal anastomoses in a porcine model. | 2008 | 18721018 |
| epicardial pulmonary vein isolation: a long-term histologic and imaging animal study comparing cryothermy versus radiofrequency. | simplifications of the cox-maze procedure to treat atrial fibrillation (af) include epicardial pulmonary vein (pv) isolation using various sources to create lines of conduction block. the creation of transmural lesions has been a concern. the heat-sink effect caused by blood flow has limited the effectiveness of unipolar radiofrequency and linear application of cryothermy, which should be abolished when clamp devices are used for pv isolation. the efficacy and safety of epicardial beating-heart ... | 2008 | 18721573 |
| [frequency of chromosomes carrying endogenous retroviruses in the populations of domestic pig and wild boar]. | statistical analysis of the frequency of chromosomes carrying three types (a, b, and c) of porcine endogenous retroviruses (perv) was based on molecular-genetic testing of populations of domestic pigs and wild boars. domestic pigs were shown to have higher frequency of perv and haplotypes containing two and three types of perv than wild boars. | 2008 | 18727389 |
| patterns of crystallin distribution in porcine eye lenses. | to measure the protein distribution patterns in single young porcine lenses. | 2008 | 18615203 |
| domestic pigs have low susceptibility to h5n1 highly pathogenic avian influenza viruses. | genetic reassortment of h5n1 highly pathogenic avian influenza viruses (hpai) with currently circulating human influenza a strains is one possibility that could lead to efficient human-to-human transmissibility. domestic pigs which are susceptible to infection with both human and avian influenza a viruses are one of the natural hosts where such reassortment events could occur. virological, histological and serological features of h5n1 virus infection in pigs were characterized in this study. two ... | 2008 | 18617994 |
| serological evidence for hepatitis e virus infection in laboratory monkeys and pigs in animal facilities in japan. | in laboratory animal facilities, monkeys and pigs are used for animal experiments, but the details of hepatitis e virus (hev) infection in these animals are unknown. the risk of infection from laboratory animals to humans has become a concern; therefore, much attention should be paid to the handling of these animals during their care and use, including surgical procedures performed on infected animals. in this connection, serum samples collected from 916 monkeys and 77 pigs kept in 23 animal fac ... | 2008 | 18633159 |
| the differences between venous air embolism and fat embolism in routine intraoperative monitoring methods, transesophageal echocardiography, and fatal volume in pigs. | venous air embolism (vae) or fat embolism (fe) may occur in similar clinical settings such as after multiple injuries or total hip replacement. we designed this study to observe the differences between vae and fe in routine intraoperative monitoring methods, transesophageal echocardiography (tee), and fatal volume in pigs. | 2008 | 18636024 |
| expression of mitochondrial transcription factor a (tfam) during porcine gametogenesis and preimplantation embryo development. | mitochondrial transcription factor a (tfam) is responsible for stability, maintenance, and transcriptional control of mitochondrial dna (mtdna). we have studied the expression and distribution of tfam in the gametes and preimplantation embryos of the domestic pig (sus scrofa). we hypothesized that tfam is not present in the boar sperm mitochondria to reduce the possibility of paternal mtdna propagation in the progeny. in contrast, we anticipated that tfam gene is expressed in a developmental sta ... | 2008 | 18636550 |
| detection of trichinella spiralis, t. britovi and t. pseudospiralis in muscle tissue with real-time pcr. | infections caused by trichinella species occur throughout the world in many wild and domestic animals resulting in trichinellosis in men. in europe, domestic pigs are predominantly infected by three trichinella species: t. spiralis, t. britovi and t. pseudospiralis. present methods for detection of trichinella spp. (compressorium method, artificial digestion) do not always sufficiently recognize trichinella larvae and these techniques are labor-intensive, time consuming and do not differentiate ... | 2008 | 18639594 |
| the effect of pest control on the incidence of trichinosis in virovitica-podravina county, croatia. | the aim of the study was to assess the role of pest control as a preventive measure in the control of trichinosis. the investigation was designed as a retrospective cohort study in the virovitica-podravina county. the incidence of trichinosis was determined according to the presence or absence of exposure to protective pest control measures. differences in the rate of trichinosis infection were determined by calculating relative risk (rr), i.e., the ratio of absolute risk in the two groups. in t ... | 2008 | 18644676 |
| comparison of selected skin decontaminant products and regimens against vx in domestic swine. | an anesthetized domestic swine model was used to compare the efficacy and cross-contamination potential of selected skin decontaminant products and regimens against the chemical warfare agent, vx. animals topically exposed to 2x, 3x or 5x ld(50) vx showed typical signs of organophosphate nerve agent poisoning, including miosis, salivation, mastication, dysrhythmias, and respiratory distress prior to death. animals were exposed to 5x ld(50) vx and then decontaminated 45 min later with the reactiv ... | 2008 | 18650258 |
| guidelines for the identification and development of sampling methods and design of suitable protocols for monitoring of trichinella infection in indicator species. | summary: according to the european community regulation no. 2075/2005, a risk-based program should be implemented in those areas where wildlife and pig holdings applying for trichinella-free status coexist, or for region where the risk of trichinella in domestic swine is officially recognised as negligible. trichinella nematode species are primarily parasites of wildlife which can transmit the infection to pigs intended for human consumption, acting as reservoir of the parasite. since not all ma ... | 2008 | 18660570 |
| insect succession and decomposition patterns on shaded and sunlit carrion in saskatchewan in three different seasons. | a study was conducted on decomposition and insect succession in the prairie ecozone of saskatchewan in the year 2000. eighteen domestic pig carcasses (42-79 kg) were employed as human models for applications to future homicide investigations in this region. two major variables were considered including the effect of season and habitat (sun versus shade). research was conducted over 25 weeks, spanning three seasons: spring, summer and fall. ambient temperature, internal carcass temperature, fauni ... | 2008 | 18662603 |
| does selective intubation increase ablation zone size during pulmonary cryoablation? | to determine the effect of selective intubation on ablation zone size during pulmonary cryoablation. | 2008 | 18662888 |
| influence of ventilation strategies on survival in severe controlled hemorrhagic shock. | to investigate the effect of different ventilation settings on hemodynamic stability in severe controlled hemorrhagic shock. | 2008 | 18679111 |
| endoscopic ultrasound-guided ethanol injection in the pancreas in a porcine model: a preliminary study. | despite aggressive multimodal treatments, survival rates for patients with pancreatic cancer remain disappointing. local progression is problematic, and minimally invasive procedures allowing locoregional control are needed. in this study, we attempted endoscopic ultrasound (eus)-guided injection of ethanol into the pancreas. | 2008 | 18702683 |
| carbon dioxide contrast agent for ct arteriography: results in a porcine model. | to test the feasibility of carbon dioxide (co(2))-enhanced computed tomographic (ct)-arteriography in an animal model. | 2008 | 18589320 |
| development of real-time polymerase chain reaction assays for rapid detection and differentiation of wild-type pseudorabies and gene-deleted vaccine viruses. | the successful eradication of pseudorabies in u.s. domestic swine was accomplished through the use of glycoprotein e (ge) deleted modified live virus vaccines and an accompanying ge differential enzyme-linked immunosorbent assay (elisa). yet, pseudorabies virus (prv) was established in feral swine in the united states, becoming a potential reservoir of prv for infection of domestic swine and other native wildlife. a critical need for the current prv surveillance program in the united states is t ... | 2008 | 18599848 |
| acalculous and clostridial cholecystitis in a pig. | a 21-month-old domestic hanford pig (sus scrofa domestica) in a 1-year study for experimental myocardial infarction was euthanized at the end of the study. one week earlier, the animal had symptoms and elevated clinical chemistry results suggestive of hepatobiliary disease, which resolved after medical therapy. at necropsy, the gallbladder was markedly enlarged, discolored, and had a thickened wall. within the gallbladder, there was abundant friable green-brown material. a culture of the gallbla ... | 2008 | 18599865 |
| molecular identification and prevalence of isospora sp. in pigs in western australia using a pcr-rflp assay. | little is known about the prevalence of isospora in domestic pigs in western australia. a total of 289 pig faecal samples were collected from pre- and post-weaned pigs and sows from 1 indoor and 3 outdoor piggeries located in the south-west region of western australia. faecal samples were screened using a pcr-rflp assay based on the its-1 rdna locus. an overall prevalence of 10.4% (30/289) was identified. isospora was detected in 16.3% (20/123) of pre-weaned animals and 6.4% (10/156) of post-wea ... | 2008 | 18601925 |
| radiofrequency hepatic ablation with internally cooled electrodes and hybrid applicators with distant saline infusion using an in vivo porcine model. | radiofrequency ablation (rfa) of tumors by means of internally cooled (ice) or multitined expandable electrodes combined with infusion of saline into the tissue may improve results. our aim was to determine the efficacy of a previously optimized hybrid ice system (ice combined with infusion of saline into the tissue at a distance of 2mm) in comparison with a conventional ice cluster electrode in porcine liver in vivo. | 2008 | 18042500 |
| topography of the porcine epicardiac nerve plexus as revealed by histochemistry for acetylcholinesterase. | the aim of the present study was to map the topography of the porcine epicardiac nerve plexus (enp) and to re-examine the total number and distribution of the porcine intracardiac ganglia and neurons. eleven juvenile pigs (sus scrofa domestica, 3-4 weeks of age) were examined employing histochemistry for acetylcholinesterase to reveal the enp on total hearts. the nerves entered porcine epicardium at five sites: (1) ventro-medially to the origin of the superior vena cava, (2) dorsally to the orig ... | 2008 | 18063424 |
| non-robotic thoracoscopic internal mammary artery preparation in the pig. a training model. | notwithstanding non-robotic, thoracoscopic preparation of the internal mammary artery (ima) is a difficult surgical task, an appropriate experimental training model is lacking. we evaluated the young domestic pig for this purpose. four domestic female pigs (30-40 kg body weight) were used for this study. bilateral thoracoscopic preparation of the ima was carried out under continuous, pressure controlled co(2) insufflation. a 30 degrees rigid thoracoscope was inserted through a 10-mm port in the ... | 2008 | 18502784 |
| [therapeutic effect of vacuum-assisted closure technology on infected explosion wound of pig]. | to investigate the effect of vacuum-assisted closure (v. a. c.) technology in the treatment of infected wound of skin and soft tissue as a result of explosion injury in pig. | 2008 | 18512552 |
| sex-related variation in compact bone microstructure of the femoral diaphysis in juvenile rabbits. | while gross morphological changes in the skeleton between males and females are well know, differences between sexes in the histomorphology are less known. it is important to have knowledge on the bone structure of rabbits, as this is a widely used species in biomedical research. a study was performed to evaluate the association between sex and the compact bone morphology of the femoral diaphysis in juvenile rabbits. | 2008 | 18522730 |
| nutritional skewing of conceptus sex in sheep: effects of a maternal diet enriched in rumen-protected polyunsaturated fatty acids (pufa). | evolutionary theory suggests that in polygynous mammalian species females in better body condition should produce more sons than daughters. few controlled studies have however tested this hypothesis and controversy exists as to whether body condition score or maternal diet is in fact the determining factor of offspring sex. here, we examined whether maternal diet, specifically increased n-6 polyunsaturated fatty acid (pufa) intake, of ewes with a constant body condition score around the time of ... | 2008 | 18541015 |
| [an investigation of the protective effect of prostaglandin e1 liposome on acute lung injury in pig]. | to assess the protective effect of prostaglandin e1 liposome (lipo-pge1) on acute lung injury (ali) in a porcine model of ali. | 2008 | 18549714 |
| pharmacokinetics of artesunate in the domestic pig. | the aim was to study the pharmacokinetic profile of artesunate and its metabolite dihydroartemisinin (dha) in a pig model. | 2008 | 18559353 |
| long beneficial effects after lipo-prostaglandin e1 administration on the ischemic gastric tube in pigs. | lipo-prostaglandin e1 (pge1) is a new preparation of pge1 in which it is bound to lipids in order to slow pge1 release and delay its rate of metabolism. we investigated how long the beneficial effects of intravenous administration of lipo-pge1 on the ischemic gastric tube continue. the gastric tube was constructed using 15 domestic pigs under general anesthesia and saline, unmodified pge1 and lipo-pge1 were infused continuously at a rate of 0.05 microg/kg/min for 10 minutes. tissue blood flow (t ... | 2008 | 18564164 |
| post-resuscitation hemodynamics and relationship to the duration of ventricular fibrillation. | we have observed consistent hemodynamic patterns after restoration of spontaneous circulation (rosc) after ventricular fibrillation (vf) cardiac arrest. we sought to characterize the time-course of these patterns, and to determine whether these differed based on duration of the vf insult. | 2008 | 18573588 |
| dynamic chemical characteristics of soil solution after pig manure application: a column study. | when manures from intensive livestock operations are applied to agricultural or vegetable fields at a high rate, large amounts of salts and metals will be introduced into soils. using a column leaching experiment, this study assessed the leaching potential of the downward movement of cu and zn as well as some salt ions after an intensive farm pig manure at rates of 0%, 5% and 10% (w/w) were applied to the top 20 cm of two different textured soils (g soil -sandy loam soil; h soil-silty clay loam ... | 2008 | 18576224 |
| renal injury and the application of polysaccharide hemospheres: a laparoscopic experimental model. | microporous polysaccharide hemospheres (mph) are hemostatic beads engineered from purified plant starch. mph accelerates the natural clotting cascade by concentrating clotting factors and proteins on their surface while absorbing aqueous and low molecular weight components from blood. the purpose of this study was to determine the efficacy of mph in achieving hemostasis in the setting of laparoscopic renal injury. | 2008 | 18578666 |
| detection and surveillance for animal trichinellosis in gb. | the zoonotic disease trichinellosis is considered one of the re-emerging diseases with surveillance and control methods constantly gaining more importance worldwide. recent change in european union (eu) legislation introduces trichinella-free production, and the possibility of risk-based monitoring for trichinella in pigs. this has increased the role of wildlife surveillance programmes and their impact on protecting human health as well as highlighted the need for harmonised surveillance protoco ... | 2008 | 18160221 |
| variability and action mechanism of a family of anticomplement proteins in ixodes ricinus. | ticks are blood feeding arachnids that characteristically take a long blood meal. they must therefore counteract host defence mechanisms such as hemostasis, inflammation and the immune response. this is achieved by expressing batteries of salivary proteins coded by multigene families. | 2008 | 18167559 |
| epidemiology of trichinellosis in germany, 1996-2006. | the epidemiology of trichinellosis in germany was investigated by analyzing national surveillance data from 1996-2006. during this period, 95 laboratory-confirmed infections and 12 outbreaks were reported. the highest mean annual incidence was found in immigrants from southeast european countries (0.3 cases/100,000 vs. 0.01 cases/100,000 in the german population) with an incidence rate ratio of 26.0 (95% confidence interval 11.6-51.8). the predominant clinical symptom was muscle pain in 79 patie ... | 2008 | 18171103 |
| effect of liquid swine manure rate, incorporation, and timing of rainfall on phosphorus loss with surface runoff. | excessive manure phosphorus (p) application increases risk of p loss from fields. this study assessed total runoff p (tpr), bioavailable p (bap), and dissolved reactive p (drp) concentrations and loads in surface runoff after liquid swine (sus scrofa domesticus) manure application with or without incorporation into soil and different timing of rainfall. four replicated manure p treatments were applied in 2002 and in 2003 to two iowa soils testing low in p managed with corn (zea mays l.)-soybean ... | 2008 | 18178885 |
| bone regeneration in osseous defects-application of particulated human and bovine materials. | different bone substitute materials are used to manage the challenge of local bone loss subsequent to craniofacial reconstructive surgery. in this animal study we examined the de novo bone formation in bone defects after insertion of puros allograft of human origin or navigraft of bovine origin, and compared the regenerative potential of each material to that of autogenous bone. | 2008 | 18206408 |
| a subpopulation of capsaicin-sensitive porcine dorsal root ganglion neurons is lacking hyperpolarization-activated cyclic nucleotide-gated channels. | hyperpolarization-activated cyclic nucleotide-gated (hcn) channels contribute to stabilizing resting membrane potential, thus controlling neuron excitability. subclasses of nociceptive neurons differ in their excitability, therefore, these channels could be a distinguishing marker. we investigated isolated dorsal root ganglion neurons from a non-rodent species, the pig, sus scrofa domesticus. single labeling revealed capsaicin-induced cobalt-uptake in 54.3% and transient receptor potential v1 (t ... | 2008 | 18218331 |
| genetic imprinting of h19 and igf2 in domestic pigs (sus scrofa). | the genes insulin-like growth factor 2 (igf2) and h19 express paternally and maternally, respectively, in humans, mice, sheep, and cattle. additionally, igf2 has been shown to be regulated by at least four promoters in a tissue- or development-specific manner. in the domestic pigs, the promoter- and tissue-specific imprinting pattern of igf2 has not been well characterized, nor is the imprinting pattern of h19. in the present study, we identified two polymorphisms in each of igf2 (exons 2 and 9) ... | 2008 | 18228173 |
| snipork - a microarray of snps in candidate genes potentially associated with pork yield and quality - development and validation in commercial breeds. | snipork is an oligonucleotide microarray based on the arrayed primer extension (apex) technique, allowing genotyping of single nucleotide polymorphisms (snps) in genes of interest for pork yield and quality traits. apex consists of a sequencing reaction primed by an oligonucleotide anchored with its 5' end to a glass slide and terminating one nucleotide before the polymorphic site. extension with one fluorescently labeled dideoxynucleotide complementary to the template reveals the polymorphism. ... | 2008 | 18228175 |
| prostasome-like vesicles stimulate acrosome reaction of pig spermatozoa. | the presence of small membranous particles characterizes the male genital fluids of different mammalian species. the influence of semen vesicles, denominated prostasomes, on sperm functional properties has been well documented in humans, but their biological activity is scarcely known in other species. the present work investigated prostasome-like vesicles in pig semen for their ability to interact with spermatozoa and to affect acrosome reaction. | 2008 | 18234073 |
| the contribution of social effects to heritable variation in finishing traits of domestic pigs (sus scrofa). | social interactions among individuals are ubiquitous both in animals and in plants, and in natural as well as domestic populations. these interactions affect both the direction and the magnitude of responses to selection and are a key factor in evolutionary success of species and in the design of breeding schemes in agriculture. at present, however, very little is known of the contribution of social effects to heritable variance in trait values. here we present estimates of the direct and social ... | 2008 | 18245326 |
| selection in the making: a worldwide survey of haplotypic diversity around a causative mutation in porcine igf2. | domestic species allow us to study dramatic evolutionary changes at an accelerated rate due to the effectiveness of modern breeding techniques and the availability of breeds that have undergone distinct selection pressures. we present a worldwide survey of haplotype variability around a known causative mutation in porcine gene igf2, which increases lean content. we genotyped 34 snps spanning 27 kb in 237 domestic pigs and 162 wild boars. although the selective process had wiped out variability f ... | 2008 | 18245828 |
| setup and dosimetry for exposing anaesthetised pigs in vivo to 900 mhz gsm mobile phone fields. | the aim of this study was a dosimetrical analysis of the setup used in the exposure of the heads of domestic pigs to gsm-modulated radio frequency electromagnetic fields (rf-emf) at 900 mhz. the heads of pigs were irradiated with a half wave dipole using three different exposure routines; short bursts of 1-3 s at two different exposure levels and a continuous 10-min exposure. the electroencephalogram (eeg) was registered continuously during the exposures to search for rf-emf originated changes. ... | 2008 | 18247366 |
| a comparative study on the effects of tylosin on select bacteria during continuous flow culture of mixed populations of gut microflora derived from a feral and a domestic pig. | continuous flow cultures of feral (culture fc) and domesticated (culture rpcf) pig gut microflora were established in steady state. cultures were continuously infused with 25 or 100 microg tylosin/ml and sampled at intervals to assess effects on total culturable anaerobes, bacteroides and enterococcus via plating to agar supplemented without or with 100 microg tylosin/ml, the latter to assess bacterial sensitivity to tylosin. endogenous tylosin-insensitive anaerobes within the cultures, while si ... | 2008 | 18260812 |
| pharmacological analyses of two naturally occurring porcine melanocortin-4 receptor mutations in domestic pigs. | the melanocortin-4 receptor (mc4r) is critical in regulating mammalian food intake and energy expenditure. numerous mutations in the mc4r gene have been identified from obese humans. so far two naturally occurring porcine mc4r (pmc4r) mutations, d298n and r236h, have been identified from various strains of pigs and d298n is being utilized as a genetic marker to screen performance traits of pigs. in this study, we performed functional analyses of pmc4r d298n and r236h, including their ligand bind ... | 2008 | 18262384 |
| development of a transoral fundoplication device and related experimental research. | this paper describes a device and surgical techniques developed between 1999 and 2003 to enable an entirely transoral approach to fundoplication. the endofundoplication system (efs) system consisted of a multifunctional flexible tube for oral introduction (18 mm) as the key component, with a specially designed retroverted grasper that was used to grasp the lower esophageal sphincter (les) area of the esophagus, for invaginating the les into the stomach and folding the gastric wall onto the wall ... | 2008 | 18270877 |
| cerebral cortical microvascular flow during and following cardiopulmonary resuscitation after short duration of cardiac arrest. | to examine changes in cerebral cortical macro- and microcirculation and their relationship to the severity of brain ischaemia during and following resuscitation from a short duration of cardiac arrest. | 2008 | 18280632 |
| concepts for visualization of multidirectional phase-contrast mri of the heart and large thoracic vessels. | multidirectional phase-contrast magnetic resonance imaging allows the acquisition of time-resolved velocity fields (vectors) of cardiac and vascular blood flow. its unique ability to provide vectorial flow information promises to give new insights into hemodynamic physiology. however, up to now appropriate and standardized procedures and software tools are missing to take advantage of all the information contained in the data. the objective of this work is to present a new versatile software too ... | 2008 | 18280934 |
| caspase-3 activation and dna damage in pig skin organ culture after solar irradiation. | in the present study, a convenient and easy-to-handle skin organ culture was developed from domestic pig ears using polycarbonate transwell culture inserts in 12-well plate. this alternative model was then tested for its suitability in analyzing the short-term effects of a single solar radiation dose (from 55 to 275 kj.m(-2)). differentiation of the pig skin was maintained for up to 48 h in culture, and its morphology was similar to that of fresh human skin. solar irradiation induced a significa ... | 2008 | 18282184 |
| tissue response to surgical energy devices. | there is little data available on the effects of energy-based surgical devices (esd) on tissues other than arteries and veins. as such, we quantified the lateral thermal damage associated with contemporary esd: the harmonic ace, a prototype bipolar device known as the gyrus trisector, the harmonic lcs-c5, and the ligasure v. | 2008 | 18289646 |
| microwave ablation with triaxial antennas tuned for lung: results in an in vivo porcine model. | to prospectively determine in swine the size and shape of coagulation zones created in normal lung tissue by using small-diameter triaxial microwave antennas and to prospectively quantify the effects of bronchial occlusion and multiple antennas on the coagulation zone. | 2008 | 18292471 |
| the adult göttingen minipig as a model for chronic heart failure after myocardial infarction: focus on cardiovascular imaging and regenerative therapies. | porcine models have become increasingly popular in cardiovascular research. the standard farm pig rapidly increases in body weight and size, potentially confounding serial measurements of cardiac function and morphology. we developed an adult porcine model that does not show physiologic increases in heart mass during the study period and is suitable for long-term study. we compared adult minipigs with the commonly used adolescent yorkshire swine. myocardial infarction was induced in adult göttin ... | 2008 | 19149414 |
| bama miniature pigs (sus scrofa domestica) as a model for drug evaluation for humans: comparison of in vitro metabolism and in vivo pharmacokinetics of lovastatin. | the objective of this study was to demonstrate that bama miniature pigs are a suitable experimental animal model for the evaluation of drugs for man. to this end, in vitro lovastatin metabolism at the minipig liver microsomal level and in vivo pharmacokinetics were studied. results were compared with those obtained from humans. our data indicate that the main metabolites and enzyme kinetic parameters of lovastatin metabolism are similar in pigs and humans. triacetyloleandomycin, a specific inhib ... | 2008 | 19149415 |
| evaluation of shape and size changes of bone and remodelled bone substitute after different fixation methods. | suitable tissue fixation is indispensable to histological analysis. this investigation, therefore, sought to evaluate changes of shape and size of bone specimens and remodelled bone substitute material following different fixation methods. mandibular bones of 9 pigs (sus scrofa domesticus) served as specimens. two mandibular premolars were extracted respectively and the extraction alveoli were filled with synthetic bone substitute material. the samples were collected after 70 days. fixation of 6 ... | 2008 | 19075329 |
| soft tissue integration in the neck area of titanium implants--an animal trial. | dental implant materials are required to enable good apposition of bone and soft tissues. they must show sufficient resistance to chemical, physical and biological stress in the oral cavity to achieve good long-term outcomes. a critical issue is the apposition of the soft tissues, as they have provided a quasi-physiological closure of oral cavity. the present experiment was performed to study the peri-implant tissue response to non-submerged (1-stage) implant installation procedures. two differe ... | 2008 | 19075332 |
| probing of actinobacillus pleuropneumoniae apxiiia toxin-dependent cytotoxicity towards mammalian peripheral blood mononucleated cells. | abstract: | 2008 | 19046441 |
| african swine fever virus isolate, georgia, 2007. | african swine fever (asf) is widespread in africa but is rarely introduced to other continents. in june 2007, asf was confirmed in the caucasus region of georgia, and it has since spread to neighboring countries. dna fragments amplified from the genome of the isolates from domestic pigs in georgia in 2007 were sequenced and compared with other asf virus (asfv) isolates to establish the genotype of the virus. sequences were obtained from 4 genome regions, including part of the gene b646l that enc ... | 2008 | 19046509 |
| candidate new species of kobuvirus in porcine hosts. | 2008 | 19046542 | |
| diffuse spectral fundus reflectance measured using subretinally placed spectralon. | the diffuse fundus reflectance and the spectral transmittance of the swine sensory retina was measured in vivo using intravitreal illumination. pars plana vitrectomy and intravitreal manipulations were performed on a female american yorkshire domestic swine. light from a scanning monochromator was coupled into a fiber optic intraocular illuminator inserted into the vitreous. a 1.93-mm(2) region of the illuminated fundus was imaged from an oblique illumination angle. multispectral retinal images ... | 2008 | 19021332 |
| occurrence of moniezia expansa (rud, 1810) blanchard, 1891 (cestoda: anoplocephalidae) in domestic pig (sus scrofa domestica linnaeus, 1758) in perú. | the presence of moniezia expansa (rud, 1810) blanchard, 1891, is reported in domestic pig (sus scrofa domestica linnaeus, 1758). four tapeworms were collected and identified as m. expansa. this is the first report of m. expansa collected in a domestic pig in perú. | 2008 | 19028016 |
| ivabradine induces an increase in ventricular fibrillation threshold during acute myocardial ischemia: an experimental study. | tachycardia often facilitates ischemic ventricular fibrillation (vf). | 2008 | 19034029 |
| expression, cellular localization and antibody responses of the african swine fever virus genes b602l and k205r. | previously, we identified serological immunodeterminants of african swine fever virus (asfv), including pk205r and pb602l, without homologues in the database. pk205r is expressed as a 33-kd protein from 4 h post-infection onward, initially diffusely distributed throughout cells, and subsequently in viral factories. pk205r was not found in purified virus. both pk205r and pb602l are recognised by hyperimmune antisera from domestic pigs and bushpigs at late time points after infection, suggesting t ... | 2008 | 19011730 |
| transvenous intramyocardial cellular delivery increases retention in comparison to intracoronary delivery in a porcine model of acute myocardial infarction. | clinical trials using intracoronary (ic) delivery of cells have addressed efficacy but the optimal delivery technique is unknown. our study aimed to determine whether transvenous intramyocardial (tvim) approach was advantageous for cellular retention in ami. | 2008 | 19012733 |
| isolation and characterization of perv-c env from domestic pig in korea. | clone perv-c (a3) env was isolated from the genomic dna of domestic pig (sus scrofa domesticus) in korea to investigate the molecular properties of perv-c. the nucleic acid homologies between the perv-msl (type c) reference and the perv-c(a3) clone was 99% for env, but a single base pair deletion was found in the transmembrane (tm) region of the env open reading frame. to examine the functional characteristics of truncated perv-c env, we constructed a replication-incompetent retroviral vector by ... | 2008 | 18955828 |
| detection and genetic characterization of porcine circovirus 2 isolates from the first cases of postweaning multisystemic and wasting syndrome in wild boars in greece. | in 2002, postweaning multisystemic wasting syndrome (pmws) was diagnosed in a european female wild boar (sus scrofa), based on the detection of porcine circovirus 2 (pcv2) dna in various organs, including the uterus, and on histopathologic lesions. this is the first detection of pcv2 dna in the uterus of a wild boar. three years later (2005), a wild boar < 6-8 mo of age was found moribund. it presented wasting and dyspnea and finally died. pcv2 dna was detected in tissue samples, and histopathol ... | 2008 | 18957642 |
| domestic pigs and japanese encephalitis virus infection, australia. | to determine whether relocating domestic pigs, the amplifying host of japanese encephalitis virus (jev), decreased the risk for jev transmission to humans in northern australia, we collected mosquitoes for virus detection. detection of jev in mosquitoes after pig relocation indicates that pig relocation did not eliminate jev risk. | 2008 | 18976557 |
| [experimental study on a new method for colonoscopic closure of large-bowel perforations with the otsc clip]. | iatrogenic perforation of the colon during interventional endoscopic procedures, e.g., mucosectomy, is a problematic complication, as safe treatment often requires surgical repair. iatrogenic perforation of the colon is indeed a rare complication; however, given the increasing case numbers in interventional endoscopy it is of considerable practical relevance. the closure of perforations can be achieved with conventional endoscopic clips; however, these working channel based clips are often too s ... | 2008 | 18979620 |
| the environmental and public health risks associated with arsenical use in animal feeds. | arsenic exposures contribute significantly to the burden of preventable disease worldwide, specifically related to increased risks of cancer, diabetes, and cardiovascular disease. most exposures are associated with natural contamination of groundwater, which is difficult to mitigate when these sources are used for drinking water. an anthropogenic source of arsenic exposure stems from the widespread use of arsenical drugs in food-animal production in the united states and china, among many countr ... | 2008 | 18991934 |
| platelets from diabetic pigs exhibit hypersensitivity to thrombin. | responses of platelets from diabetic and diabetic-hyperlipidemic pigs were studied. pigs were made diabetic with single dose of alloxan, which acts by selectively destroying insulin-producing pancreatic beta cells thus inducing type 1 diabetes. pigs were kept for 1 or 12 wk, during which thrombin-induced aggregation was monitored in washed platelets. the platelets showed increased sensitivity to aggregation within 1 wk of induction of diabetes. hyperlipidemia alone for 12 wk did not increase pla ... | 2008 | 19004374 |
| current world status of balantidium coli. | balantidium coli is a cosmopolitan parasitic-opportunistic pathogen that can be found throughout the world. pigs are its reservoir hosts, and humans become infected through direct or indirect contact with pigs. in rural areas and in some developing countries where pig and human fecal matter contaminates the water supply, there is a greater likelihood that balantidiosis may develop in humans. the infection may be subclinical in humans, as it mostly is in pigs, or may develop as a fulminant infect ... | 2008 | 18854484 |
| optical coherence tomography and intravascular ultrasound imaging of bioabsorbable magnesium stent degradation in porcine coronary arteries. | absorbable metallic stents (ams) composed of magnesium alloy were designed to complete degradation within 90-120 days. among the potential advantages of these stents, when compared to conventional stents, are the elimination of late stent thrombosis, chronic inflammation, and artifacts during noninvasive imaging. | 2008 | 18928950 |
| continuous hemofiltration in pigs with hyperdynamic septic shock affects cardiac repolarization. | sepsis has been defined as the systemic host response to infection with an overwhelming systemic production of both proinflammatory and anti-inflammatory mediators. continuous hemofiltration has been suggested as possible therapeutic option that may remove the inflammatory mediators. however, hemodialysis and hemofiltration were reported to influence cardiac electrophysiologic parameters and to increase the arrhythmogenic risk. we hypothesize that sepsis affects electrophysiologic properties of ... | 2008 | 18936693 |
| purification, molecular cloning, heterologous expression and characterization of pig cyp1a2. | porcine cytochrome p450 (cyp) 1a2 was purified to electrophoretic homogeneity from the hepatic microsomes of beta-naphthoflavone-treated male pigs. in a reconstituted system, this enzyme showed a good catalytic activity towards caffeine, acetanilide, and methoxyresorufin, all known markers of mammalian cyp1a2. using 3'- and 5'-rapid amplification of coding dna (cdna) ends (race), we amplified from the liver rna of control pigs a full-length 1827 bp cdna containing an open reading frame of 1548 b ... | 2008 | 18949657 |