Publications

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alterations in host metabolism by the specific and anorectic effects of the cattle tick (boophilus microplus). i. food intake and body weight growth. 19715280977
alterations in host metabolism by the specific and anorectic effects of the cattle-tick (boophilus microplus). ii. changes in blood composition. 19715280978
a modification to the collection of saliva from boophilus microplus. 19715552701
esterases and a carbohydrate-splitting enzyme in the saliva of the cattle tick, boophilus microplus. 19715553457
bovine babesiasis: estimation of infection rates in the tick vector boophilus microplus (canestrini). 19714999518
an experimental investigation into the effects of flooding on the ixodid tick boophilus microplus (canestrini).the effect of water immersion on the non-parasitic stages of boophilus microplus were investigated both under laboratory and field conditions. survival of engorged female ticks was influenced by their age and the temperature of the water. eggs and larvae were more resistant to submerison than were engorged females and their survival was increased at low temperatures and in water with high oxygen content. the persistence of this tick in areas prone to flooding was attributed to the survival of pa ...197128310970
new types of organophosphorus-resistant ticks (boophilus microplus). 19715102657
the mechanism of feeding and salivation in boophilus microplus (canestrini, 1887). 19715565392
development and survival of the tick boophilus microplus (can.) in laboratory and under natural conditions of cuba. 19715163210
observations on the dispersal of larvae of the cattle tick boophilus microplus (can.). 19705450439
the absorption and metabolism of (14c)ddt in ddt-resistant and susceptible strains of the cattle tick boophilus microplus. 19705475727
the efficacy of supona (chlorfenvinphos) against cattle ticks in nigeria under field conditions. 19705535686
[study on the ticks of chejudo-life cycle]this study was conducted to study the life cycle of haemaphysalis bispinosa and boophilus microplus. the results obtained are summarized as follows. 1. the period of blood-sucking from a host was 20-25 days (average 22.5 days) for haemaphysalis bispinosa and was 28-43 days (average 35.5 days) for boophilus microplus. 2. the parasitism period of haemaphysalis bispinosa on the host was the same as the blood sucking period, but the parasitism period of boophilus microplus was only 20-23 days (avera ...197012913514
white-tailed deer as hosts of cattle fever-ticks.a penned study for obtaining definitive information on the status of white-tailed deer (odocoileus virginianus) as a host for cattle feverticks (boophilus microplus) was conducted on st. croix of the u.s. virgin islands. four generations of fever-ticks were propagated on one deer during a six month period. nine wild white-tailed deer also were collected from four insular estates to evaluate the carrier status of these animals on an island where cattle fever-ticks are indigenous. two deer were in ...197016512152
[fine structure of the merozoites of babesia bigemina in the ovary of boophilus microplus and boophilus decoloratus]. 19694988938
cattle tick in new south wales. 19694936858
boophilus microplus (acarina: ixodidae): experimental infestations on cattle restrained from grooming. 19695401459
metabolism of aniline in the cattle tick boophilus microplus canestrini. 19695781640
the ecology of cattle ticks in bugisu, uganda. 19695789101
the production of cuticle wax by engorged females of the cattle tick, boophilus microplus (canestrini). 19695793885
the effect of cattle tick (boophilus microplus)on production of brahman-cross and british-breed cattle in northern australia. 19695814104
effect of fungal extracts on the cattle tick, boophilus microplus. 19695814109
boophilus microplus: antihistaminic and tranquillizing drugs and cattle resistance. 19694396008
boophilus microplus: antihistaminic and tranquilizing drugs and cattle resistance. 19694396213
cholesterol in the cuticular wax of boophilus microplus. 19695786195
insecticides for control of the cattle tick and the southern cattle tick on cattle. 19685642113
mechanism of resistance to organophosphorus acaricides in a strain of the cattle tick boophilus microplus. 19685643270
inheritance of resistance to organophosphorus acaricides in the cattle tick, boophilus microplus. 19685666452
brain cholinesterase activity and its inheritance in cattle tick (boophilus microplus) strains resistant and susceptible to organophosphorus acaricides. 19685666453
the efficacy and stability of dursban insecticide in dipping vat for control of the southern cattle tick. 19685666957
acetylcholinesterase insensitivity in the biarra strain of the cattle tick boophilus microplus, as a cause of resistance to organophosphorus and carbamate acaricides. 19685680996
loss of toxicity to cattle ticks of a wettable powder formulation of coumaphos. 19685689244
eradication of cattle tick (boophilus microplus) from magnetic island, queensland, in the presence of native fauna. 19685693053
developments in the resistance status of the southern cattle tick to organophosphorus and carbamate insecticides. 19685723760
the feeding processes of the cattle tick boophilus microplus (canestrini). ii. the sequence of host-tissue changes. 19685740485
resistance of cattle to the tick boophilus microplus (canestrini). i. development of ticks on bos taurus. 19685761112
resistance of cattle to the tick boophilus microplus (canestrini). ii. stages of the life cycle of the parasite against which resistance is manifest. 19685761113
the infection of boophilus microplus with babesia bigemina. 19684984380
[a study of the cattle ticks in guadelope and martinique. ii. pathogenic agents transmitted by ticks]. 19675628907
acaricide tests on the biarra strain of organophosphorus resistant cattle tick boophilus microplus from southern queensland. 19676067998
investigations into the cold resistance of the eggs and larvae of boophilus decoloratus (koch, 1844), boophilus microplus (canestrini, 1888) and margaropus winthemi karsch, 1879. 19676069321
acaricide resistance and cattle tick control. 19676069461
acaricide resistance and cattle tick control. 19676069462
ixodes nipponensis n. sp. (ixodoidea, ixodidae), a common cattle tick in japan. 19676078267
[further observations in madagascar on the resistance of the tick boophilus microplus (canestrini) to insecticides of the chlorinated hydrocarbon family]. 19675614032
[study of livestock ticks in guadeloupe and martinique. 3. resistance of the boophilus microplus tick to gammexane in the french antilles]. 19674177893
culture of an organophosphorus-resistant strain of boophilus microplus (can.) and an assessment of its resistance spectrum. 19665945563
[study on cattle ticks in guadelupe and martinique. i. ticks and their distribution (acarina, ixodoidea)]. 19665951733
metabolism of coumaphos in larvae of the cattle tick boophilus microplus. 19665970060
the feeding processes of the cattle-tick boophilus microplus (canestrini): a study in host-parasite relations. i. attachment to the host. 19665971582
loss of infectivity of a vaccine strain of babesia argentina for boophilus microplus. 19664961183
the cattle tick and tick fever. 196514338020
pathogenesis in the cattle tick, boophilus microplus. 196314089936
sulphydryl content of the embryos of the australian cattle tick. 196014439556
studies of cuticular lipides of arthropods. iii. the chemical composition of the wax from boophilus microplus. 195713425627
a further note on the use of synthetic insecticides in the control of cattle tick. (boophilus microplus canestrini): some preliminary observations with "497". 195014772186
gammexane and cattle ticks. 194818911585
recent developments in the control of cattle tick and buffalo-fly. 194720262399
a new method for the control of cattle ticks in tropical regions. 194621015596
d.d.t. and cattle ticks. 194521010551
interaction of plant essential oil terpenoids with the southern cattle tick tyramine receptor: a potential biopesticide target.an outbreak of the southern cattle tick, rhipicephalus (boophilus) microplus, (canestrini), in the united states would have devastating consequences on the cattle industry. tick populations have developed resistance to current acaricides, highlighting the need to identify new biochemical targets along with new chemistry. furthermore, acaricide resistance could further hamper control of tick populations during an outbreak. botanically-based compounds may provide a safe alternative for efficacious ...027986436
acaricidal efficacies of lippia gracilis essential oil and its phytochemicals against organophosphate-resistant and susceptible strains of rhipicephalus (boophilus) microplus.plant-derived natural products can serve as an alternative to synthetic compounds for control of ticks of veterinary and medical importance. lippia gracilis is an aromatic plant that produces essential oil with high content of carvacrol and thymol monoterpenes. these monoterpenes have high acaricidal activity against rhipicephalus (boophilus) microplus. however, there are no studies that show efficacy differences of essential oils between susceptible and organophosphate resistant strains of r. ( ...027692332
assessment of the repellent effect of lippia alba essential oil and major monoterpenes on the cattle tick rhipicephalus microplus.the control of rhipicephalus microplus (ixodida: ixodidae) is achieved using synthetic acaricides. however, resistant tick populations are widespread around the world. plant essential oils can act as repellents, keeping ticks away from hosts and decreasing the selection pressure on synthetic acaricides. the aim of this study was to evaluate the in vitro repellent effect of lippia alba essential oil on r. microplus larvae. leaves from two l. alba genotypes maintained under the same agronomic and ...026471008
diversity and convergence of sodium channel mutations involved in resistance to pyrethroids.pyrethroid insecticides target voltage-gated sodium channels, which are critical for electrical signaling in the nervous system. the intensive use of pyrethroids in controlling arthropod pests and disease vectors has led to many instances of pyrethroid resistance around the globe. in the past two decades, studies have identified a large number of sodium channel mutations that are associated with resistance to pyrethroids. the purpose of this review is to summarize both common and unique sodium c ...024019556
update on tick-borne rickettsioses around the world: a geographic approach.tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus rickettsia. these zoonoses are among the oldest known vector-borne diseases. however, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. several species of tick-borne rickettsiae that wer ...024092850
modeling and biological control of mosquitoes.models can be useful at many different levels when considering complex issues such as biological control of mosquitoes. at an early stage, exploratory models are valuable in exploring the characteristics of an ideal biological control agent and for guidance in data collection. when more data are available, models can be used to explore alternative control strategies and the likelihood of success. there are few modeling studies that explicitly consider biological control in mosquitoes; however, t ...017853610
in-vitro assessment of the acaricidal properties of artemisia annua and zataria multiflora essential oils to control cattle ticks.the aim of this study was to investigate the 'acaricidal effect' of zataria multiflora and artemisia annua essential oils on rhipicephalus (boophilus) annulatus.022347275
recent advances in single-cell maldi mass spectrometry imaging and potential clinical impact.single-cell analysis is gaining popularity in the field of mass spectrometry as a method for analyzing protein and peptide content in cells. the spatial resolution of maldi mass spectrometry (ms) imaging is by a large extent limited by the laser focal diameter and the displacement of analytes during matrix deposition. owing to recent advancements in both laser optics and matrix deposition methods, spatial resolution on the order of a single eukaryotic cell is now achievable by maldi ms imaging. ...021999830
anaplasma phagocytophilum infection in domestic animals in ten provinces/cities of china.a nationwide epidemiologic investigation of domestic animal infections has been conducted in nine provinces and one city during 2007-2010. serum samples from a total of 707 goats, 433 cattle, and 219 dogs were collected for detecting anaplasma phagocytophilum igg antibody by immunofluorescence assays and the average seroprevalences were 10.05% for dogs, 3.82% for goats, and 0.69% for cattle, respectively. a total of 472 goats, 201 cattle, 102 dog blood clots, and 1,580 ticks were collected for p ...022764312
severe fever with thrombocytopenia syndrome in patients suspected of having scrub typhus.to determine prevalence of severe fever with thrombocytopenia syndrome in south korea, we examined serum samples from patients with fever and insect bite history in scrub typhus-endemic areas. during the 2013 scrub typhus season, prevalence of this syndrome among patients suspected of having scrub typhus was high (23.0%), suggesting possible co-infection.027767909
haemaphysalis longicornis ticks as reservoir and vector of severe fever with thrombocytopenia syndrome virus in china.severe fever with thrombocytopenia syndrome (sfts) is an emerging hemorrhagic fever in east asia caused by sfts virus (sftsv), a newly discovered phlebovirus. the haemaphysalis longicornis tick has been suspected to be the vector of sftsv. to determine whether sftsv can be transmitted among ticks, from ticks to animals, and from animals to ticks, we conducted transmission studies between developmental stages of h. longicornis ticks and between ticks and mice. using reverse transcription pcr, we ...026402039
severe fever with thrombocytopenia syndrome in japan and public health communication.a fatal case of severe fever with thrombocytopenia syndrome was reported in japan in 2013. the ensuing process of public communication offers lessons on how to balance public health needs with patient privacy and highlights the importance of multilateral collaborations between scientific and political communities.025695132
general properties and phylogenetic utilities of nuclear ribosomal dna and mitochondrial dna commonly used in molecular systematics.to choose one or more appropriate molecular markers or gene regions for resolving a particular systematic question among the organisms at a certain categorical level is still a very difficult process. the primary goal of this review, therefore, is to provide a theoretical information in choosing one or more molecular markers or gene regions by illustrating general properties and phylogenetic utilities of nuclear ribosomal dna (rdna) and mitochondrial dna (mtdna) that have been most commonly used ...010634037
theileria, babesia, and anaplasma detected by pcr in ruminant herds at bié province, angola.distribution of anaplasma spp., babesia spp., theileria spp., and ehrlichia ruminantium, was for the first time studied in bié province, central angola. we examined 76 blood samples of cattle originated from seven farms, and 13 blood samples of goats from two farms employing molecular genetic tools (pcr). most prevalent was a. ovis-infection in goats (100%) and a. marginale-infection in cattle (38% of examined animals, and six out of seven farms). b. bigemina-infection was detected in only one s ...023193527
molecular cloning and characterization of an acetylcholinesterase cdna in the brown planthopper, nilaparvata lugens.a full cdna encoding an acetylcholinesterase (ache, ec 3.1.1.7) was cloned and characterized from the brown planthopper, nilaparvata lugens stål (hemiptera: delphacidae). the complete cdna (2467 bp) contains a 1938-bp open reading frame encoding 646 amino acid residues. the amino acid sequence of the ache deduced from the cdna consists of 30 residues for a putative signal peptide and 616 residues for the mature protein with a predicted molecular weight of 69,418. the three residues (ser242, glu3 ...020874389
therapeutic uses and pharmacological properties of garlic, shallot, and their biologically active compounds.garlic (allium sativum l. family liliaceae) is well known in iran and its leaves, flowers, and cloves have been used in traditional medicine for a long time. research in recent decades has shown widespread pharmacological effects of a. sativum and its organosulfur compounds especially allicin. studies carried out on the chemical composition of the plant show that the most important constituents of this plant are organosulfur compounds such as allicin, diallyl disulphide, s-allylcysteine, and dia ...024379960
an insight into the microbiome of the amblyomma maculatum (acari: ixodidae).the aim of this study was to survey the bacterial diversity of amblyomma maculatum koch, 1844, and characterize its infection with rickettsia parkeri. pyrosequencing of the bacterial 16s rrna was used to determine the total bacterial population in a. maculatum. pyrosequencing analysis identified rickettsia in a. maculatum midguts, salivary glands, and saliva, which indicates successful trafficking in the arthropod vector. the identity of rickettsia spp. was determined based on sequencing the ric ...024605461
using the dfci gene index databases for biological discovery.the dfci gene index web pages provide access to analyses of ests and gene sequences for nearly 114 species, as well as a number of resources derived from these. each species-specific database is presented using a common format with a home page. a variety of methods exist that allow users to search each species-specific database. methods implemented currently include nucleotide or protein sequence queries using wu-blast, text-based searches using various sequence identifiers, searches by gene, ti ...020205187
in vitro effect of the ethanolic extract of tephrosia vogelii on rhipicephalus sanguineus in abomey-calavi.ticks are vectors of several diseases, of which many are zoonosis transmissible to humans. the use of tephrosia leafs' extract as a low cost acaricide is spreading among farmers in central kenya.026101758
severe fever with thrombocytopenia syndrome virus in ticks collected from humans, south korea, 2013.we investigated the infection rate for severe fever with thrombocytopenia syndrome virus (sftsv) among ticks collected from humans during may-october 2013 in south korea. haemaphysalis longicornis ticks have been considered the sftsv vector. however, we detected the virus in h. longicornis, amblyomma testudinarium, and ixodes nipponensis ticks, indicating additional potential sftsv vectors.025061851
rickettsia and vector biodiversity of spotted fever focus, atlantic rain forest biome, brazil. 024565262
inhibitor profile of bis(n)-tacrines and n-methylcarbamates on acetylcholinesterase from rhipicephalus (boophilus) microplus and phlebotomus papatasi.the cattle tick, rhipicephalus (boophilus) microplus (bm), and the sand fly, phlebotomus papatasi (pp), are disease vectors to cattle and humans, respectively. the purpose of this study was to characterize the inhibitor profile of acetylcholinesterases from bm (bmache1) and pp (ppache) compared to human and bovine ache, in order to identify divergent pharmacology that might lead to selective inhibitors. results indicate that bmache has low sensitivity (ic50 = 200 μm) toward tacrine, a monovalent ...024187393
importance of ticks and their chemical and immunological control in livestock.the medical and economic importance of ticks has long been recognized due to their ability to transmit diseases to humans and animals. ticks cause great economic losses to livestock, and adversely affect livestock hosts in several ways. loss of blood is a direct effect of ticks acting as potential vector for haemo-protozoa and helminth parasites. blood sucking by large numbers of ticks causes reduction in live weight and anemia among domestic animals, while their bites also reduce the quality of ...017048307
amblyomma americanum tick calreticulin binds c1q but does not inhibit activation of the classical complement cascade.in this study we characterized amblyomma americanum (aam) tick calreticulin (crt) homolog in tick feeding physiology. in nature, different tick species can be found feeding on the same animal host. this suggests that different tick species found feeding on the same host can modulate the same host anti-tick defense pathways to successfully feed. from this perspective it's plausible that different tick species can utilize universally conserved proteins such as crt to regulate and facilitate feedin ...025454607
neuropeptides and neuropeptide receptors in the drosophila melanogaster genome.recent genetic analyses in worms, flies, and mammals illustrate the importance of bioactive peptides in controlling numerous complex behaviors, such as feeding and circadian locomotion. to pursue a comprehensive genetic analysis of bioactive peptide signaling, we have scanned the recently completed drosophila genome sequence for g protein-coupled receptors sensitive to bioactive peptides (peptide gpcrs). here we describe 44 genes that represent the vast majority, and perhaps all, of the peptide ...011381038
purification and properties of a novel nucleotide-hydrolysing enzyme (5'-nucleotidase) from boophilus microplus.the tick boophilus microplus contains a nucleoside phosphate-hydrolysing enzyme which, in many respects, resembles the well characterized 5'-nucleotidase from mammalian tissue. the tick enzyme has been purified to homogeneity. it is a membrane-bound glycoprotein with an apparent mr of 67,000 and, although it fails to hydrolyse a range of nucleoside 2'- or 3'-monophosphates, it has broad specificity for the 5' derivatives. further investigation of the enzyme's substrate specificity, however, show ...02539105
cloning and immunoreactivity of the 5-ht 1mac and 5-ht 2mac receptors in the central nervous system of the freshwater prawn macrobrachium rosenbergii.biogenic amines are implicated in several mental disorders, many of which involve social interactions. simple model systems, such as crustaceans, are often more amenable than vertebrates for studying mechanisms underlying behaviors. although various cellular responses of biogenic amines have been characterized in crustaceans, the mechanisms linking these molecules to behavior remain largely unknown. observed effects of serotonin receptor agonists and antagonists in abdomen posture, escape respon ...019184976
identification of a cluster of residues in transmembrane segment 6 of domain iii of the cockroach sodium channel essential for the action of pyrethroid insecticides.a phenylalanine residue (phe1519) in the sixth transmembrane segment of domain iii (iiis6) of the cockroach bgna(v) sodium channel is required for the binding and action of pyrethroids. however, whether or not other residues in iiis6 participate in the action of pyrethroids remains to be determined. in the present study, we conducted a systematic analysis of 20 residues in iiis6 of the bgna(v) channel using alanine-scanning mutagenesis. our results show that alanine substitutions of four residue ...019154185
use of polymerase chain reaction: restriction fragment length polymorphism to detect acaricidal resistance to synthetic pyrethroids in boophilus microplus ticks of south india.boophilus microplus is an important ectoparasite of livestock. apart from transmitting diseases, heavy tick burden can decrease production and damage hides. the synthetic pyrethroids which are advantageous over other acaricides for treatment of this infestation are now losing their efficacy due to development of resistant strains of ticks.023961446
rapid concerted evolution in animal mitochondrial dna.recombinational genetic processes are thought to be rare in the uniparentally inherited mitochondrial (mt) dna molecules of vertebrates and other animals. here, however, we document extremely rapid concerted microevolution, probably mediated by frequent gene conversion events, of duplicated sequences in the mtdna control region of mangrove killifishes (kryptolebias marmoratus). in local populations, genetic distances between paralogous loci within an individual were typically smaller (and often ...017490947
proteome changes in the plasma of papilio xuthus (lepidoptera: papilionidae): effect of parasitization by the endoparasitic wasp pteromalus puparum (hymenoptera: pteromalidae).although the biochemical dissection of parasitoid-host interactions is becoming well characterized, the molecular knowledge concerning them is minimal. in order to understand the molecular bases of the host immune response to parasitoid attack, we explored the response of papilio xuthus parasitized by the endoparasitic wasp pteromalus puparum using proteomic approach. by examining the differential expression of plasma proteins in the parasitized and unparasitized host pupae by two-dimensional (2 ...019489110
current advances in detection and treatment of babesiosis.babesiosis is a disease with a world-wide distribution affecting many species of mammals principally cattle and man. the major impact occurs in the cattle industry where bovine babesiosis has had a huge economic effect due to loss of meat and beef production of infected animals and death. nowadays to those costs there must be added the high cost of tick control, disease detection, prevention and treatment. in almost a century and a quarter since the first report of the disease, the truth is: the ...022360483
but2 is a member of the third major group of hat transposons and is involved in horizontal transfer events in the genus drosophila.the hat superfamily comprises a large and diverse array of dna transposons found in all supergroups of eukaryotes. here we characterized the drosophila buzzatii but2 element and found that it harbors a five-exon gene encoding a 643-aa putatively functional transposase. a phylogeny built with 85 hat transposases yielded, in addition to the two major groups already described, ac and buster, a third one comprising 20 sequences that includes but2, tip100, hat-4_bm, and rp-hat1. this third group is h ...024459285
genetic diversity of tick-borne rickettsial pathogens; insights gained from distant strains.the ability to capture genetic variation with unprecedented resolution improves our understanding of bacterial populations and their ability to cause disease. the goal of the pathogenomics era is to define genetic diversity that results in disease. despite the economic losses caused by vector-borne bacteria in the order rickettsiales, little is known about the genetic variants responsible for observed phenotypes. the tick-transmitted rickettsial pathogen anaplasma marginale infects cattle in tro ...025364572
development of a spatially targeted field sampling technique for the southern cattle tick, rhipicephalus microplus, by mapping white-tailed deer, odocoileus virginianus, habitat in south texas.the objective of our study was to determine whether satellite remote sensed data could be used to identify white-tailed deer, odocoileus virginianus (zimmerman) (artiodactyla: cervidae), habitat and target locations for sampling free-living larvae of the southern cattle tick, rhipicephalus (boophilus) microplus (canestrini) (ixodida: ixodidae) in south texas. two methods for mapping white-tailed deer habitat were used, an object-oriented method to identify closed canopies and waterways for deer ...025368044
evolutionary process of bos taurus cattle in favourable versus unfavourable environments and its implications for genetic selection.the evolutionary processes that have enabled bos taurus cattle to establish around the globe are at the core to the future success of livestock production. our study focuses on the history of cattle domestication including the last 60 years of b. taurus breeding programmes in both favourable and unfavourable environments and its consequences on evolution and fitness of cattle. we discuss the emergence of 'production diseases' in temperate production systems and consider the evolutionary genetics ...025567936
cloning and sequence analysis of chitin synthase gene fragments of demodex mites.to our knowledge, few reports on demodex studied at the molecular level are available at present. in this study our group, for the first time, cloned, sequenced and analyzed the chitin synthase (chs) gene fragments of demodex folliculorum, demodex brevis, and demodex canis (three isolates from each species) from xi'an china, by designing specific primers based on the only partial sequence of the chs gene of d. canis from japan, retrieved from genbank. results show that amplification was successf ...023024043
demographic fitness of belminus ferroae (hemiptera: triatominae) on three different hosts under laboratory conditions.triatominae are widely recognised for their role as vectors of trypanosoma cruzi. one of the main biological characteristics of this subfamily is their obligate haematophagous condition. however, previous studies on belminus herreri and belminus ferroae suggested that cockroaches are their principal hosts in domiciles. due to this peculiar behaviour, the aim of this study was to analyse several demographic and reproductive parameters of b. ferroae fed on three different hosts (mice, cockroaches ...024141961
the global invertebrate genomics alliance (giga): developing community resources to study diverse invertebrate genomes.over 95% of all metazoan (animal) species comprise the "invertebrates," but very few genomes from these organisms have been sequenced. we have, therefore, formed a "global invertebrate genomics alliance" (giga). our intent is to build a collaborative network of diverse scientists to tackle major challenges (e.g., species selection, sample collection and storage, sequence assembly, annotation, analytical tools) associated with genome/transcriptome sequencing across a large taxonomic spectrum. we ...024336862
purification and studies on characteristics of cholinesterases from daphnia magna.due to their significant value in both economy and ecology, daphnia had long been employed to investigate in vivo response of cholinesterase (che) in anticholinesterase exposures, whereas the type constitution and property of the enzyme remained unclear. a type of che was purified from daphnia magna using a three-step procedure, i.e., triton x-100 extraction, ammonium sulfate precipitation, and diethylaminoethyl (deae)-sepharose™-fast-flow chromatography. according to sodium dodecyl sulfate poly ...023549850
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