Publications

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the purification and properties of butyryl-coenzyme a dehydrogenase from peptostreptococcus elsdenii.butyryl-coa dehydrogenase prepared by a simple procedure from peptostreptococcus elsdenii has a molecular weight of approx. 150000. the enzyme has fad as its prosthetic group. the amino acid analysis is reported. this enzyme, like most of the corresponding mammalian ones, is green. the absorption band at 710nm can be abolished irreversibly by dithionite reduction and air reoxidation; it can be abolished reversibly by phenylmercuric acetate or potassium bromide. the enzyme as isolated appears to ...19715145910
green butyryl-coenzyme a dehydrogenase. an enzyme-acyl-coenzyme a complex.1. butyryl-coa dehydrogenase from peptostreptococcus elsdenii forms very tightly bound complexes with various acyl-coa compounds. spectra in some cases merely show resolution of the 450nm band, but those with acetoacetyl-, pent-2-enoyl- and 4-methylpent-2-enoyl-coa show long-wavelength bands similar to the 710nm band of native enzyme. these complexes are formed instantaneously by the yellow form of the enzyme and much more slowly by the green form. 2. an acid extract of the green enzyme reconver ...19715145911
carbon dioxide requirement of various species of rumen bacteria.the carbon dioxide requirement of 32 strains of rumen bacteria, representing 11 different species, was studied in detail. increasing concentrations of co(2) were added as nahco(3) to a specially prepared co(2)-free medium which was tubed and inoculated under nitrogen. prior depletion of co(2) in the inoculum was found to affect the level of requirement; however, the complexity and buffering capacity of the medium did not appear to be involved. an absolute requirement for co(2) was observed for e ...19715541030
[sensitivity test of clinical isolates to fusidic acid]. 19714397858
isolation of anaerobic bacteria from clinical specimens. 19704314713
[species and antibiotic sensitivity of anaerobes isolated from various clinical materials in the last 8 years]. 19705311041
[subacute bacterial endocarditis due to peptostreptococcus]. 19705458522
diagnosis and treatment of bacteroides and peptostreptococcal infections. 19705429732
peptostreptococcus elsdenii from the caecum of pigs. 19705516605
the tryptophans in flavodoxin and synthetic flavinyl peptides characterized by chemical and photochemical oxidations. 19705417473
the isolation and properties of the predominant anaerobic bacteria in the caeca of chickens and turkeys. 19704920052
the microflora of the pig stomach and its possible relationship to ulceration of the pars oesophagea. 19704920613
phenylethyl alcohol agar medium for isolation of anaerobic bacteria. 19704927090
pectin-fermenting bacteria isolated from the bovine rumen.thirty-two strains of pectin-fermenting rumen bacteria were isolated from bovine rumen contents in a rumen fluid medium which contained pectin as the only added energy source. based on differences in morphology and the gram stain, 10 of these strains were selected for characterization. two strains were identified as lachnospira multiparus, four strains were identified as butyrivbrio fibrisolvens, and three strains were identified as bacteroides ruminicola. characteristics of the remaining strain ...19695802604
isoleucine biosynthesis from 2-methylbutyric acid by anaerobic bacteria from the rumen.microorganisms in ruminal ingesta and pure cultures of anaerobic ruminal bacteria of different physiological and morphological groups incorporated (14)c from labeled 2-methylbutyrate during growth. the radioactivity was incorporated mainly into lipid and protein. isoleucine was the only labeled amino acid found in acid hydrolysates of protein from either pure or mixed cultures. radioactivity in isoleucine synthesized from 2-methylbutyrate-1-(14)c was entirely in carbon-2. thus, the carboxylation ...19695813342
purification and characterization of flavodoxin from peptostreptococcus elsdenii. 19694976788
oxygen sensitivity of various anaerobic bacteria.anaerobes differ in their sensitivity to oxygen, as two patterns were recognizable in the organisms included in this study. strict anaerobes were species incapable of agar surface growth at po(2) levels greater than 0.5%. species that were found to be strict anaerobes were treponema macrodentium, treponema denticola, treponema oralis n. sp., clostridium haemolyticum, selenomonas ruminatium, butyrivibrio fibrisolvens, succinivibrio dextrinosolvens, and lachnospira multiparus. moderate anaerobes w ...19695370458
[subacute bacterial endocarditis causes by anaerobic peptostreptococcus with brief review of general clinical picture]. 19695377254
isolation and identification of rumen bacteria capable of anaerobic rutin degradation. 19695392469
[case of peptostreptococcal subacute bacterial endocarditis with pda]. 19695307945
acute and recurrent thromboembolic disease: a new concept of etiology. 19695344031
a study of cultures of rumen anaerobic bacteria in the presence of excess riboflavine (vitamin b2). 19694901038
spectrophotometric titration of ferredoxins and chromatium high potential iron protein with sodium dithionite. 19694306283
oxidation-reduction properties of flavodoxin from peptostreptococcus elsdenii. 19694388813
complex formation between ferredoxin triphosphopyridine nucleotide reductase and electron transfer proteins. 19694388814
[on the chemical composition of the cell wall of streptococci. 3. the amino acid sequence of a glycine-containing murein from peptostreptococcus evolutus (prevot) smith]. 19684236672
non-heme iron proteins. v. the amino acid sequence of rubredoxin from peptostreptococcus elsdenii. 19685640967
[pathogenic significance of anaerobes especially non-sporulating anaerobes, in infection]. 19685672990
enzymic studies on the biosynthesis of amino acids from lactate by peptostreptococcus elsdenii.cell-free extracts of peptostreptococcus elsdenii, a strict anaerobe from the rumen, were examined for enzymes catalysing the steps in the biosynthesis from lactate of alanine, serine, aspartate and glutamate. extracts contain the enzymes necessary for the formation of alanine from lactate via pyruvate. the presence of enzymes catalysing the interconversion of phosphoglycerate and phosphohydroxypyruvate, the transamination of the latter to phosphoserine and the cleavage of phosphoserine to serin ...19685690537
[anaerobic bacteria isolated from the sputum and nasopharyngeal fluids]. 19685717385
adjunctive use of antibiotics in traumatic dental procedures. 19684972570
[studies on the occurrence and the frequency of various microorganisms (anaerobic bacteria, trichomonads, mycoplasmas and yeasts) in the human vagina in vaginitis]. 19675629109
tracer studies on the biosynthesis of amino acids from lactate by peptostreptococcus elsdenii.peptostreptococcus elsdenii, a strict anaerobe from the rumen, was grown on a medium containing yeast extract and [1-(14)c]- or [2-(14)c]-lactate. radioisotope from lactate was found in all cell fractions, but mainly in the protein. the label in the protein fraction was largely confined to a few amino acids: alanine, serine, aspartic acid, glutamic acid and diaminopimelic acid. the alanine, serine, aspartic acid and glutamic acid were separated, purified and degraded to establish the distributio ...19676069834
chemical composition and serological analysis of the cell wall of peptostreptococcus.bahn, arthur n. (northwestern university, chicago, ill.), patrick c. y. kung, and james a. hayashi. chemical composition and serological analysis of the cell wall of peptostreptococcus. j. bacteriol. 91:1672-1676. 1966.-chemical and serological analyses were made of the cell wall of peptostreptococcus to characterize taxonomically this genus of anaerobic streptococci. cell wall hydrolysates of p. putridus strains 06 and 85, p. intermedius strains 11 and 87, and p. elsdenii strain b-159 were prep ...19665937229
studies of the predominant cultivable micro-organisms from the human tongue. 19665225869
importance of the isovalerate carboxylation pathway of leucine biosynthesis in the rumen.certain anaerobic ruminal bacteria synthesize the leucine carbon skeleton by use of a pathway different from that described in other microorganisms. these organisms carboxylate the intact carbon skeleton of isovalerate, synthesizing leucine-2-c(14) from isovalerate-1-c(14). strains of bacteroides ruminicola and peptostreptococcus elsdenii were like ruminococcus flavefaciens in that they incorporated appreciable amounts of c(14) from isovalerate-1-c(14) into cellular protein and in that the only ...19665970468
[adansonian taxonomy and the relationship of microorganisms in terms of the concepts of similarity value and center species. (2). on the tribe streptococcae of the family coccaceae]. 19666005767
anaerobic bacteria in routine urine culture.one hundred and fifty-eight anaerobic organisms from 147 patients were isolated from 15,250 consecutive clean mid-stream or catheter urine specimens. the pathogenicity of the anaerobic genera commonly isolated from urine is reviewed and discussed. failure to establish anaerobic isolates as pathogens and a paucity of reported cases proving anaerobic bacteria as significant causes of urinary tract infection permitted discontinuation of anaerobic culture as part of the routine screening procedure f ...19664288917
effect of antibiotics on some rumen and intestinal bacteria. 19664162813
enzymatic studies of pure cultures of rumen microorganisms.joyner, a. e., jr. (university of california, davis), and r. l. baldwin. enzymatic studies of pure cultures of rumen microorganisms. j. bacteriol. 92:1321-1330. 1966.-the activities of enzymes representing the major pathways of carbohydrate metabolism and anaerobic electron transport in cell-free extracts of whole rumen contents have been reported. the effects of diet upon the activities of several enzymes suggested that enzymatic measurements might prove useful for the study of rumen metabolism ...19664380801
fermentation of lactic acid by the rumen microorganism, peptostreptococcus elsdenii. 19655216438
lactate metabolism by peptostreptococcus elsdenii: evidence for lactyl coenzyme a dehydrase. 196514292829
establishment of human indigenous bacteria in germ-free mice.gibbons, r. j. (forsyth dental center and harvard school of dental medicine, boston, mass.), s. s. socransky, and b. kapsimalis. establishment of human indigenous bacteria in germ-free mice. j. bacteriol. 88:1316-1323. 1964.-thirteen strains of bacteria indigenous to the gingival crevice area of man were tested for their ability to establish as monocontaminants in germ-free mice. three facultative organisms, streptococcus mitis, staphylococcus albus, and a "diphtheroid," established, as well as ...196414234787
use of phenethyl alcohol in media for isolation of anaerobic bacteria. 196414240975
electron transport in peptostreptococcus elsdenii. 196414269334
the gingival crevice microbiota in five preschool children. 196414126698
studies of the predominant cultivable microbiota of dental plaque. 196414170653
[contribution to the study of the biosynthesis of fatty acids. ii. fermentation of lactic acid c-2 by peptostreptococcus elsdenii]. 196314079882
[acetate kinase in cell free extracts of pepostreptococcus elsdenii]. 196314089091
[about the systematic classification of peptostreptococcus elsdenii]. 196314089092
role of ferredoxin in the metabolism of molecular hydrogen.valentine, r. c. (university of illinois, urbana) and r. s. wolfe. role of ferredoxin in the metabolism of molecular hydrogen. j. bacteriol. 85:1114-1120. 1963.-the metabolism of molecular hydrogen by clostridium pasteurianum, micrococcus lactilyticus (veillonella alcalescens), and several other anaerobic bacteria was studied. oxidation of hydrogen, using several electron-accepting substrates including triphosphopyridine nucleotide, uric acid, xanthine, nitrite, and hydroxylamine, required ferre ...196314044002
[contribution to the study of the biosynthesis of fatty acids. i. studies carried out with washed peptostreptococcus elsdenni cells]. 196213974660
some nutritional characteristics of predominant culturable ruminal bacteria.bryant, m. p. (u.s. department of agriculture, beltsville, md.) and i. m. robinson. some nutritional characteristics of predominant culturable ruminal bacteria. j. bacteriol. 84:605-614. 1962.-the effect of enzymatic hydrolysate of casein, nh(4) (+), a mixture of volatile fatty acids (acetic, n-valeric, isovaleric, 2-methylbutyric, and isobutyric), hemin, and ruminal fluid on growth of 89 freshly isolated strains of predominant culturable ruminal bacteria was studied, using basal media containin ...196214016429
biological characteristics of an obligate anaerobic amylolytic coccus. 196014435124
bacterial changes in the rumen during the onset of feed-lot bloat of cattle and characteristics of peptostreptococcus elsdenii n. sp. 195913617940
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