Publications

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h2-receptor antagonists and gastric cancer. 20102878342
the smoking gun. 20102857863
[the polymerase chain reaction]. 20102797295
analysis of ligand-binding data without knowledge of bound or free ligand molar concentration.a method is proposed to set the parameters in a nonlinear regression procedure to determine the equilibrium dissociation constant (kd) and the high affinity receptor concentration (bmax) of systems consisting of one ligand, one high affinity receptor, and n low affinity binding sites. this method is suitable when neither bound or free ligand formal concentrations nor the maximum of the binding signal can be deduced from the experimental data. the method makes use of (i) the abscissa of the first ...20103218740
distal sensory nerves of the lower extremity in peripheral neuropathy: comparison of medial dorsal cutaneous and sural nerve abnormalities.this study investigates the sensitivity and usefulness of medial dorsal cutaneous nerve (mdcn) conduction studies in patients with peripheral neuropathy. two hundred twenty-three patients with clinical signs and symptoms of peripheral neuropathy in their lower extremities were evaluated. nerve conduction velocity studies of the mdcn were compared to those of the sural and peroneal motor nerves. fifty-eight percent of the patients had no measurable sensory nerve action potential (snap) from eithe ...20102981521
beta-endorphin: surface binding and internalization in thymoma cells.the opioid peptide beta-endorphin binds to specific nonopioid binding sites (mr 72,000) that are present on the surface of thymoma cells. beta-endorphin is then internalized, apparently via the mr 72,000 species, and is subsequently found within intracellular, vesicular structures. this process is accompanied by the down-regulation of the mr 72,000 binding sites. our findings suggest that beta-endorphin may modulate cellular functions, such as t-lymphocyte proliferation, at intracellular rather ...20102994047
[health status of workers chronically exposed to lead]. 20102987891
sequential study of vasculitis in mrl mice.the frequency, age of onset and organ distribution of spontaneously occurring vasculitis was examined in a sequential study with 170 mrl mice of both substrains. necrotizing vasculitis was seen in 55.8% of mrl/mp-lpr/lpr mice studied, beginning at the age of 3 months. the kidney and urinary bladder were most frequently involved. in mrl/mp- +/+ mice necrotizing vasculitis was much less frequently present (7.6%), beginning at the age of 18 months, and was seen only in the kidney, stomach and teste ...20102891873
[lipid metabolism in patients with pulmonary tuberculosis living in the north]. 20102958842
response to parathyroid hormone and 1,25-dihydroxyvitamin d3 of bone-derived cells isolated from normal children and children with abnormalities in skeletal development.in order to evaluate the role of intrinsic defects in osteoblast function in the pathogenesis of diseases of skeletal development, we developed techniques which permit the evaluation of the metabolic properties of bone-derived cells in vitro. cells from control children demonstrated a variety of properties classically attributed to osteoblasts (presence of alkaline phosphatase positive cells and synthesis of bone gla protein) and responded to pth (camp production) and to 1,25-dihydroxyvitamin d3 ...20103003145
[bifurcated resection in the surgical treatment of central non-small cell t3 carcinoma].reported in this paper is experience obtained since 1978 from surgical removal of the tracheal bifurcation in combination with pneumonectomy in 38 cases. squamous cell carcinoma centrally growing from the upper lobe was recorded from 24 patients to whom 36 interventions had been applied for bronchial carcinoma. 50 per cent of all operations in conjunction with pneumonectomy were performed on patients in the pt3nomo stage. the cumulated 3-year survival rate amounted to 37 per cent. mortality in h ...20103020840
how community pharmacy staff manage a patient with possible oral cancer.community pharmacy staff were surveyed for the advice they would offer to a patient with a history highly suggestive of oral carcinoma. less than 10% of 57 pharmacies indicated that a dental or medical opinion should be sought. this study confirms that the knowledge of pharmacy staff about oral disease is as limited as it is about systemic disease and suggests that a dental or medical consultation is necessary for persisting complaints.20102920160
ommatidia in the developing drosophila eye require and can respond to sevenless for only a restricted period.the sevenless gene encodes a putative cell surface receptor that is expressed in a highly specific and complex pattern in the developing drosophila eye. we show here that this pattern is not required for the development of either the r7 cell or any other cell in the retina. by transiently expressing sevenless protein, we show that there is only a brief period during eye development when the sevenless protein is required for the formation of the r7 photoreceptor. our results indicate that the spe ...20102924353
[recent management of renal cell carcinoma].our experience of radical nephrectomy was analyzed and recent management of renal cell carcinoma was reviewed. one hundred forty-eight patients with renal cell carcinoma were treated by radical nephrectomy between 1970 and december, 1986. the 5-year survival rate according to pathological t-stage was 100% for 4 patients in pt1, 73% for 85 patients in pt2, 51% for 54 patients in pt3, and 0% for 5 patients in pt4. four patients in pt1 had no venous involvement, lymph node metastasis, or distant me ...20103257677
ossified leiomyoma of the stomach: demonstration on computed tomography. 20102914197
the effect of alpha-difluoromethylornithine on natural killer cell and tumoricidal macrophage induction by interferon in vivo.the objective of the present investigation was to evaluate the effect of dfmo (dl-alpha-difluoromethylornithine hcl h2o) administration on tumoricidal effector cell generation by ifn or ifn inducers in vivo. dfmo administration reduces both splenic leukocyte and peritoneal macrophage polyamine levels. in tumor bearing (b16 melanoma) mice, dfmo administration did not impair splenic natural killer (nk) cell augmentation, assessed against nk sensitive yac-1 target cells, by ifn alpha/beta or the if ...20103086243
creativity in clinical nursing. 20102981993
[body marking in black africa]. 20102896582
h1 receptor antagonist treatment of chronic rhinitis.in patients with chronic rhinitis, h1 receptor antagonists play an important role in relieving the symptoms of sneezing, itching, and rhinorrhea. new information about the pharmacokinetics and pharmacodynamics of first-generation h1 receptor antagonists such as chlorpheniramine has become available in the past few years. comprehensive pharmacokinetic and pharmacodynamic studies of new relatively nonsedating h1 receptor antagonists such as terfenadine, astemizole, loratadine, and cetirizine are a ...20102897389
camp-mediated growth inhibition of a b-lymphoid precursor cell line reh is associated with an early transient delay in g2/m, followed by an accumulation of cells in g1.this study was undertaken to gain more insight into the effects of cyclic adenosine monophosphate (camp) on cell-cycle progression in the b-lymphoid precursor cell line reh. the adenylate cyclase activator forskolin reduced the proliferation of asynchronously growing reh cells by 50% after 72 hr culture. growth inhibition was associated with an accumulation of cells in g1. furthermore, we demonstrated that forskolin provoked a delay of cells for approximately 10 hr in g2/m prior to the g1 arrest ...20102848044
life stresses of slavery.skeletal evidence exists for life stresses of 120 black individuals from 25 sites in maryland, virginia, and the carolinas. periods for statistical comparison are eighteenth century, 1690-1770; catoctin furnace, maryland industrial slaves, 1790-ca.1820; 1800-ca.1860, nineteenth century; and a twentieth-century black sample compiled from forensic (accidental deaths) cases. from these archaeological sources, skeletal age at death shifts from 36 years, female, and 30 years, male in eighteenth centu ...20103322027
[pneumatosis cystoides intestinalis]. 20104828244
synapse formation in the olfactory cortex by regenerating optic axons: ultrastructural evidence for polyspecific chemoaffinity.the optic nerve was severed at its entry into the optic chiasma and implanted into the striatal region of the ipsilateral cerebral hemisphere in adult frogs (rana pipiens). regenerating retinal ganglion cell axons, observed by the autoradiographic tracing method and by horseradish peroxidase (hrp) fiber filling, grew anteriorly along the olfactory tracts and posteriorly along the ipsilateral lateral forebrain bundle and stria medullaris. many of the regenerating axons ultimately joined the ipsil ...20102822778
[mesoblastic nephroma].three cases of the "cellular" variant of mesoblastic nephroma are reported. a feature of one of them was prolonged (7.5 months) spontaneous development of the tumor which presented a peculiar morphologic appearance. diagnostic differentiation of mesoblastic nephroma from wilm's tumor and sarcomas is discussed. results of an electron-microscopic study of one of the tumors are given.20102823749
[coinfection by borrelia burgdorferi, francisella tularensis and coxiella burnetii in domestic animals from gansu province, china]. 201022993755
fastidious intracellular bacteria as causal agents of community-acquired pneumonia.intracellular bacteria are common causes of community-acquired pneumonia that grow poorly or not at all on standard culture media and do not respond to beta-lactam antibiotic therapy. apart from well-established agents of pneumonia such as legionella pneumophila, mycoplasma pneumoniae, chlamydia pneumoniae, chlamydia psittaci and coxiella burnetii, some new emerging pathogens have recently been recognized, mainly parachlamydia acanthamoebae and simkania negevensis, two chlamydia-related bacteria ...201020586563
coxiella burnetii infection of a steller sea lion (eumetopias jubatus) found in washington state.a pregnant sea lion stranded in the state of washington was found to have placentitis caused by a unique strain of coxiella burnetii. this is the first description of coxiellosis in a sea lion and suggests that exposure to sea lions may be a risk factor for contracting q fever.201020592144
circulating cytokines and procalcitonin in acute q fever granulomatous hepatitis with poor response to antibiotic and short-course steroid therapy: a case report.q fever is a zoonosis distributed worldwide that is caused by coxiella burnetii infection and the defervescence usually occurs within few days of appropriate antibiotic therapy. whether the changes of cytokine levels are associated with acute q fever with persistent fever despite antibiotic therapy had not been investigated before.201020594295
pentamidine inhibits coxiella burnetii growth and 23s rrna intron splicing in vitro.coxiella burnetii is the bacterial agent of q fever in humans. acute q fever generally manifests as a flu-like illness and is typically self-resolving. in contrast, chronic q fever usually presents with endocarditis and is often life-threatening without appropriate antimicrobial therapy. unfortunately, available options for the successful treatment of chronic q fever are both limited and protracted (>18 months). pentamidine, an rna splice inhibitor used to treat fungal and protozoal infections, ...201020599360
coxiella burnetii phase i and ii variants replicate with similar kinetics in degradative phagolysosome-like compartments of human macrophages.coxiella burnetii infects mononuclear phagocytes, where it directs biogenesis of a vacuolar niche termed the parasitophorous vacuole (pv). owing to its lumenal ph (approximately 5) and fusion with endolysosomal vesicles, the pv is considered phagolysosome-like. however, the degradative properties of the mature pv are unknown, and there are conflicting reports on the maturation state and growth permissiveness of pv harboring virulent phase i or avirulent phase ii c. burnetii variants in human mon ...201020515926
the first serologic study of q fever in sheep in iran.coxiella burnetii is an obligate intracellular microorganism that causes q fever in humans and animals. in ewes, c. burnetii infections are generally asymptomatic, but they can lead to abortions, stillbirths, and delivery of weak and unviable lambs. serological assays are suitable for screening herds. enzyme-linked immunosorbent assays (elisa) technique has a high sensitivity and a good specificity. the aim of this study was to investigate the presence of anti-c. burnetii antibodies among sheep ...201020521106
[q fever in wild animals in europe, attention to hunters]. 201020524406
seroprevalence of coxiella burnetii in domestic ruminants in gran canaria island, spain.coxiella burnetii is the causative agent of q fever, a zoonosis with worldwide occurrence. in the canary islands, the overall seroprevalence in humans has been estimated to be 21.5%. gran canaria island concentrates the highest ruminant population in the archipelago and the prevalence of the human infection is 23.5%. to evaluate the seroprevalence in livestock and the affected areas in gran canaria island, a total of 1249 ruminants were randomly selected for this study (733 goats, 369 sheep and ...201020537108
comprehensive diagnostic strategy for blood culture-negative endocarditis: a prospective study of 819 new cases.background. blood culture-negative endocarditis (bcne) may account for up to 31% of all cases of endocarditis. methods. we used a prospective, multimodal strategy incorporating serological, molecular, and histopathological assays to investigate specimens from 819 patients suspected of having bcne. results. diagnosis of endocarditis was first ruled out for 60 patients. among 759 patients with bcne, a causative microorganism was identified in 62.7%, and a noninfective etiology in 2.5%. blood was t ...201020540619
risk of exposure to q fever pathogen boosted by travel in iraq or netherlands. 201020551401
in this issue.you give me fever all through the night...: peggy lee probably wasn't singing about q fever, a severe but self-limiting flu-like disease in humans caused by coxiella burnetii. it also infects a wide variety of wild and domesticated animals. although it is an obligate intracellular organism, it is environmentally rather stable, resistant to many decontamination methods and a potential bio-warfare agent. commercial diagnostic tests (complement fixation, indirect immunofluorescence) require purifie ...201020552582
emerging evidence for q fever in humans in denmark: role of contact with dairy cattle.until recently, q fever was notified in very low numbers annually in denmark and it was always considered to be acquired abroad. preliminary reports now describe coxiella burnetii in milk samples from danish dairy cattle. serum samples of a large cohort of farmers, veterinarians, inseminators and hoof trimmers, all having occupational contact with dairy cattle, were tested for the presence of igg to phase i and phase ii antigens of c. burnetii. in 39 of 359 individuals studied (11%), the presenc ...201019832723
microbiological quality of milk from small processing units in senegal.consumption of milk and dairy products has increased significantly in senegal in the last decade, and a large part of the local production comes from small processing units spread all over the country. we collected 85 bulk-tank milk samples from 68 smallholder dairy farms throughout the territory. microbiological quality of milk samples was analyzed according to the official standards. further, raw milk and pasteurized milk were screened for mycobacterium bovis, coxiella burnetii, and anti-bruce ...201020141346
q fever outbreak in cheltenham, united kingdom, in 2007 and the use of dispersion modelling to investigate the possibility of airborne spread.we describe the investigation of an outbreak of q fever in the town of cheltenham, england. the outbreak was detected in june 2007, and prospective and retrospective case finding identified 30 confirmed or probable human cases. the investigation identified windborne spread of coxiella burnetii from nearby sheep farms as the most likely source of infection. a telephone survey was conducted to identify risk practices at local farms. subsequently the atmospheric dispersion model name was used to id ...201020350497
q fever in the netherlands: an update on the epidemiology and control measures.since the steady rise in human cases which started in 2007, q fever has become a major public health problem in the netherlands with 2,357 human cases notified in the year 2009. ongoing research confirms that abortion waves on dairy goat farms are the primary source of infection for humans, primarily affecting people living close (under 5 km) to such a dairy goat farm. to reverse the trend of the last three years, drastic measures have been implemented, including the large-scale culling of pregn ...201020350500
role of cd4+ and cd8+ t cells in clearance of primary pulmonary infection with coxiella burnetii.the mechanisms of the primary adaptive immune response to coxiella burnetii are not well known. following inoculation of the lungs with c. burnetii nine mile phase i (nmi), scid mice developed pneumonia and splenomegaly and succumbed to infection, whereas wild-type mice cleared the infection by 24 days. scid mice reconstituted with either cd4+ t cells or cd8+ t cells alone were able to control the infection, indicating that the presence of either type of t cells was sufficient to control infecti ...201020351144
serologic detection of a rare case of q fever in new york city having hepatic and unusual renal complications.we describe the case of a 46-year-old resident of new york city with a one-year history of frequent urination and 3 weeks of undulating fevers. he also had liver and bone marrow abnormalities where a non-culturable gram-negative rod was identified. q fever was suspected and confirmed based on highly elevated phase i and ii serum igm/igg antibodies against coxiella burnetii.201020376527
coxiella burnetii in humans and ticks in rural senegal.q fever is a worldwide zoonotic disease caused by coxiella burnetii. epidemiologically, animals are considered reservoirs and humans incidental hosts.201020386603
genome-wide profiling of humoral immune response to coxiella burnetii infection by protein microarray.comprehensive evaluation of the humoral immune response to coxiella burnetii may identify highly needed diagnostic antigens and potential subunit vaccine candidates. here we report the construction of a protein microarray containing 1901 c. burnetii orfs (84% of the entire proteome). this array was probed with q-fever patient sera and naïve controls in order to discover c. burnetii-specific seroreactive antigens. among the 21 seroreactive antigens identified, 13 were significantly more reactive ...201020391532
a large q fever outbreak in an urban school in central israel.background. on 28 june 2005, numerous cases of febrile illness were reported among 322 students and employees of a boarding high school located in an urban area in central israel. subsequent investigation identified a large outbreak of q fever which started 2 weeks earlier. we describe the investigation of this outbreak and its possible implications. methods. we conducted a case-control study to identify risk factors for q fever disease. environmental sampling was conducted to identify the sourc ...201020415568
neospora caninum and coxiella burnetii seropositivity are related to endocrine pattern changes during gestation in lactating dairy cows.q fever is a zoonotic infection caused by coxiella burnetii that is endemic worldwide. domestic ruminants are a source of infection for humans. given the suggestion that the bacterium recrudesces during pregnancy in cattle, this study was designed to determine whether c. burnetii infection affects hormonal patterns, such as progesterone, cortisol, pregnancy associated glycoproteins (pag), and prolactin during gestation in lactating cows. possible interactions with neospora caninum were also expl ...201020416940
practical method for extraction of pcr-quality dna from environmental soil samples.methods for the extraction of pcr-quality dna from environmental soil samples by using pairs of commercially available kits were evaluated. coxiella burnetii dna was detected in spiked soil samples at <1,000 genome equivalents per gram of soil and in 12 (16.4%) of 73 environmental soil samples.201020435765
[q fever: emotions and reality]. 201020437904
dna gyrase and topoisomerase iv mutations in an in vitro fluoroquinolone-resistant coxiella burnetii strain.the etiological agent of q fever, coxiella burnetii, is an obligate intracellular bacterium that multiplies within a vacuole with lysosomal characteristics. quinolones have been used as an alternative therapy for q fever. in this study, quinolone-resistance-determining regions of the genes coding for dna gyrase and topoisomerase iv were analyzed by dna sequencing from an in vitro fluoroquinolone-resistant c. burnetii strain (q212). sequencing and aligning of dna gyrase encoding genes (gyra and g ...201020438350
spread of q fever within dairy cattle herds: key parameters inferred using a bayesian approach.q fever is a worldwide zoonosis caused by coxiella burnetii. although ruminants are recognized as the most important source of human infection, no previous studies have focused on assessing the characteristics of the bacterial spread within a cattle herd and no epidemic model has been proposed in this context. we assess the key epidemiological parameters from field data in a bayesian framework that takes into account the available knowledge, missing data and the uncertainty of the observation pr ...201020444719
q fever: clinical manifestations and treatment.public awareness and advances in the diagnostic approach to q fever have provided important information on epidemiological and clinical aspects of this zoonosis. coxiella burnetii infection exhibits various acute or chronic clinical forms, and infection during pregnancy may jeopardize the integrity of the fetus. the presentation of infection is often nonspecific and this hinders prompt diagnosis. therapeutic regimens vary, and treating q fever during pregnancy and childhood is often challenging. ...201020455682
[q fever already present for a longer time in the netherlands]. 201020469578
presence of coxiella burnetii dna in the environment of the united states, 2006 to 2008.coxiella burnetii is an obligate intracellular bacterium that causes the zoonotic disease q fever. because c. burnetii is highly infectious, can survive under a variety of environmental conditions, and has been weaponized in the past, it is classified as a select agent and is considered a potential bioweapon. the agent is known to be present in domestic livestock and in wild animal populations, but the background levels of c. burnetii in the environment have not been reported. to better understa ...201020472727
the changing etiology of fever of intermediate duration.fever of intermediate duration (fid) is a common condition. currently, its most frequent causes are not well defined.201020153558
peri- and intra-operative management of the goat during acute surgical experimentation.goats are used as animal models for surgery and trauma research. the authors discuss appropriate methods for induction of anesthetics, intubation and surgical maintenance of the goat during acute experimentation. risks imposed by the q fever pathogen coxiella burnetii are described, as well as measures that have proven effective in minimizing zoonotic transmission of this pathogen to laboratory personnel. with appropriate knowledge of its applications, peri- and intra-operative management and li ...201020164949
considering q fever when working with laboratory sheep.the sheep (ovis aries) is somewhat less common than smaller species in laboratory settings, but personnel who work with sheep or in a facility that houses sheep should be aware that certain zoonotic diseases are common in sheep. they should also know how to prevent transmission of zoonotic disease in facilities that house or work with small ruminants. knowledge of diseases such as query fever (q fever), which can cause severe human morbidity (and in some cases death), needs to be especially emph ...201020164950
q fever pneumonia.q fever is a disease found in both humans and animals, caused by the bacterium coxiella burnetii. the epidemiology of q fever is that of the animal reservoirs of the infection including both direct and indirect contact and use of a variety of products from such animals as cattle, sheep, and goats. pneumonia is the major manifestation of q fever in some countries. it is mild to moderate in severity, and mortality is unusual. it can occur as sporadic or outbreak cases.201020171543
european homograft bank: twenty years of cardiovascular tissue banking and collaboration with transplant coordination in europe.established in 1989 in brussels as an international nonprofit association, the european homograft bank (ehb) has been collaborating closely with the transplant coordination of the different centers in belgium and other european countries. donor selection is made after discussion of exclusion criteria with the transplant coordinator of the procurement center. ehb collaborates with 15 belgian, 11 german, 10 french, 10 swiss, 3 italian, 3 dutch, and some other procurement and/or implantation center ...201020172310
[infection by coxiella burnetii (q fever)].in spite of being described over 60 years, q fever is still a little known disease. the exact prevalence is also unknown, but probably the number of cases of q fever is underestimated. there is much variation in the clinical presentation, including severe forms with a poor prognosis. acute cases often present as an asymptomatic infection, flu-like syndrome, pneumonia or hepatitis. presumably, host factors play an important role in the development of chronic disease, which may present as endocard ...201020172420
a dna-binding peroxiredoxin of coxiella burnetii is involved in countering oxidative stress during exponential-phase growth.coxiella burnetii is a gram-negative, obligate intracellular bacterial pathogen that resides within the harsh, acidic confines of a lysosome-like compartment of the host cell that is termed a parasitophorous vacuole. in this study, we characterized a thiol-specific peroxidase of c. burnetii that belongs to the atypical 2-cysteine subfamily of peroxiredoxins, commonly referred to as bacterioferritin comigratory proteins (bcps). coxiella bcp was initially identified as a potential dna-binding prot ...201020173000
[the fight against q fever. experts about the cleanup and the future of the goat husbandry in the netherlands]. 201020178298
review of microarray studies for host-intracellular pathogen interactions.obligate intracellular bacteria are privileged soldiers on the battlefield that represent host-pathogen interactions. microarrays are a powerful technology that can increase our knowledge about how bacteria respond to and interact with their hosts. this review summarizes the limitations inherent to host-pathogen interaction studies and essential strategies to improve microarray investigations of intracellular bacteria. we have compiled the comparative genomic and gene expression analyses of obli ...201020188126
seroprevalence of q fever in a district located in the west black sea region of turkey.q fever is a worldwide zoonosis caused by coxiella burnetii. in turkey, it has been reported from the late 1940s that q fever is endemic in humans and animals. our objective was to evaluate the seroprevalence in samsun tekkeköy (north turkey), where an outbreak of q fever occurred in 2002. in this cross-sectional study, subjects were selected by the random proportional sampling method. all subjects were healthy with no specific symptoms and tested by the microimmunofluorescent antibody test. in ...201020195671
polar localization of the coxiella burnetii type ivb secretion system.coxiella burnetii is a gram-negative pleomorphic bacterium and the causative agent of q fever. during infection, the pathogen survives and replicates within a phagosome-like parasitophorous vacuole while influencing cellular functions throughout the host cell, indicating a capacity for effector protein secretion. analysis of the c. burnetii (rsa 493 strain) genome sequence indicates that c. burnetii contains genes with homology to the legionella pneumophila dot/icm type ivb secretion system (t4b ...201020199576
q fever in greenland.we report a patient with q fever endocarditis in a settlement in eastern greenland (isortoq, ammassalik area). likely animal sources include sled dogs and seals. q fever may be underdiagnosed in arctic areas but may also represent an emerging infection.201020202433
q fever endocarditis.coxiella burnetii, the agent of q fever, produces a variety of clinical syndromes. the most frequent and serious chronic presentation is endocarditis, which presents unspecifically as a blood-culture negative endocarditis. it occurs almost exclusively in patients who have pre-existing valvular disease or who are immunocompromised. without prompt recognition and appropriate antimicrobial therapy, the course of q fever endocarditis is severe and potentially fatal. the epidemiology, signs and sympt ...201020218950
the use of a geographic information system to identify a dairy goat farm as the most likely source of an urban q-fever outbreak.a q-fever outbreak occurred in an urban area in the south of the netherlands in may 2008. the distribution and timing of cases suggested a common source. we studied the spatial relationship between the residence locations of human cases and nearby small ruminant farms, of which one dairy goat farm had experienced abortions due to q-fever since mid april 2008. a generic geographic information system (gis) was used to develop a method for source detection in the still evolving major epidemic of q- ...201020230650
a case of person-to-person transmission of q fever from an active duty serviceman to his spouse.coxiella burnetii has recently gained military relevance given its potential as a bioterrorism agent, and the multiple cases reported among u.s. military personnel deployed to the middle east. sexual transmission of q fever is rare but has been reported in the literature. we describe the possible sexual transmission of q fever from a returning serviceman from iraq to his wife. in a recent editorial commentary, dr. raoult wrote about the reemergence of q fever after september 11, 2001 (raoult 200 ...201020020811
beclin 1 modulates the anti-apoptotic activity of bcl-2: insights from a pathogen infection system.coxiella burnetii is an obligate intracellular bacterium that generates large vacuoles in which this pathogen replicates and survives. we have previously demonstrated that c. burnetii interacts with the autophagic pathway as a strategy for its survival and replication. coxiella displays an anti-apoptotic activity to maintain host cell viability, leading to a persistent infection. our recent study reveals that beclin 1 is recruited to the coxiella-membrane vacuole favoring its development and bac ...201020023428
real-time pcr with serum samples is indispensable for early diagnosis of acute q fever.the world's largest q fever outbreak is ongoing in the netherlands with around 3,000 confirmed cases since the first half of 2007. increased awareness has resulted in early referral of patients for diagnostics. an important drawback to serological diagnosis of acute q fever is the lag phase in antibody response. therefore, we evaluated the performance of a real-time pcr for detection of coxiella burnetii dna using serum samples from patients with acute q fever. pcr, targeting is1111, was retrosp ...201020032219
a survey of western australian sheep, cattle and kangaroos to determine the prevalence of coxiella burnetii.the objective of this study was to investigate the prevalence of coxiella burnetii in two domestic ruminant species (cattle and sheep) and the western grey kangaroo (macropus fuliginosus) in western australia (wa). the idexx chekit q fever elisa and cft were used to test sera from 50 sheep and 329 head of cattle for anti-c. burnetii antibodies and 343 kangaroo sera were tested using an indirect elisa developed specifically for this study. faecal or urine samples collected from the same animals w ...201020036083
mycotic splenic artery aneurysm secondary to coxiella burnetii endocarditis.mycotic artery aneurysms are rare but potentially lethal vascular lesions due to their high risk of rupture. bacterial endocarditis as well as trauma and inadequate immunity are predisposing factors. surgery remains the treatment of choice, although alternative methods have been used. we report the first known case of a 6 cm mycotic splenic artery aneurysm proximal to the splenic hilum, secondary to bacterial endocarditis from coxiella burnetii. resection of the aneurysm, splenectomy, and distal ...201020036491
proteomic screening for possible effector molecules secreted by the obligate intracellular pathogen coxiella burnetii.coxiella burnetii is a gram-negative, gamma-proteobacteria with nearly worldwide distribution, and it is the pathogenic agent of q-fever in man. it is an obligate intracellular parasite that is highly adapted to reside within the eukaryotic phagolysosome. in fact, it is the only known intracellular bacterium that manages to survive and replicate within a fully formed, acidic phagolysosome. c. burnetti possesses a functional type 4 secretion system (t4ss), similar to the dot/icm system of legione ...201020044831
fibrin ring granulomas in rickettsia typhi infection.we describe a 71-year-old man hospitalized for fever and productive cough. laboratory investigation showed anemia, thrombocytopenia, elevated transaminases, hyponatremia, and hypoalbuminemia. computerized tomography of the abdomen, thorax, and sinuses, echocardiography, and a gallium scan did not reveal the source of the fever. the patient remained febrile despite courses of piperacillin-tazobactam/azithromycin and ceftriaxone/vancomycin. a bone marrow biopsy showed fibrin ring granulomas, and 2 ...201020071129
development of a novel dna microarray to detect bacterial pathogens in patients with chronic obstructive pulmonary disease (copd).a novel microarray was constructed with dna pcr product probes targeting species specific functional genes of nine clinically significant respiratory pathogens, including the gram-positive organisms (streptococcus pneumoniae, streptococcus pyogenes), the gram-negative organisms (chlamydia pneumoniae, coxiella burnetii haemophilus spp., legionella pneumophila, moraxella catarrhalis, and pseudomonas aeruginosa), as well as the atypical bacterium, mycoplasma pneumoniae. in a "proof-of-concept" eval ...201020074591
infectious diseases. questions abound in q-fever explosion in the netherlands. 201020075230
seroepidemiological study of q fever in domestic ruminants in semi-extensive grazing systems.q fever, a worldwide zoonotic disease caused by coxiella burnetii, is endemic in northern spain where it has been reported as responsible for large series of human pneumonia cases and domestic ruminants' reproductive disorders. to investigate pathogen exposure among domestic ruminants in semi-extensive grazing systems in northern spain, a serosurvey was carried out in 1,379 sheep (42 flocks), 626 beef cattle (46 herds) and 115 goats (11 herds). serum antibodies were analysed by elisa and positiv ...201020089188
prevalence of coxiella burnetii antibodies in danish dairy herds.during recent years in denmark higher rates of antibodies to coxiella burnetii have been detected in animals and humans than previously reported. a study based on bulk tank milk samples from 100 randomly selected dairy herds was performed to estimate the prevalence and geographical distribution of antibody positive dairy herds. using the chekit q-fever antibody elisa test kit (idexx), the study demonstrated a prevalence of 59% antibody positive herds, 11% antibody intermediate herds and 30% anti ...201020092653
epstein-barr virus infection associated with bone marrow fibrin-ring granuloma.we previously reported 24 cases of marrow fibrin-ring granuloma (frg) encountered in 1 institution and concluded that, contrary to previous studies showing marrow frg as a diagnostic marker for q fever, epstein-barr virus (ebv) was the most common proven cause of such frg. the present study characterized patients with ebv-associated marrow frg. we retrospectively reviewed 17 cases of ebv-associated frg (43% of 40 cases with marrow frg) diagnosed by bone marrow biopsy. patients with ebv-associate ...201020093240
serological patterns of brucellosis, leptospirosis and q fever in bos indicus cattle in cameroon.brucellosis, leptospirosis and q fever are important infections of livestock causing a range of clinical conditions including abortions and reduced fertility. in addition, they are all important zoonotic infections infecting those who work with livestock and those who consume livestock related products such as milk, producing non-specific symptoms including fever, that are often misdiagnosed and that can lead to severe chronic disease. this study used banked sera from the adamawa region of camer ...201020098670
q fever endocarditis; not always expected.q fever endocarditis is a chronic disease with protean manifestations. the clinical and serological manifestations of nine patients diagnosed as having q fever endocarditis during a 19-year period are reviewed. four patients (44%) required valve replacement due to congestive heart failure. three of these four patients were diagnosed as having q fever endocarditis only after elective valve surgery, by histopathological examination of the valve and subsequent serological tests. prior to surgery th ...201019519847
[mycotic aneurysm of infrarenal abdominal aorta due to coxiella burnetii]. 201019573882
coxiella burnetii (q fever) seroprevalence in cattle.human cases of q fever appear to be common in northern ireland compared to the rest of the british isles. the purpose of this study was to describe the seroepidemiology of coxiella burnetii infection in cattle in northern ireland in terms of seroprevalence and determinants of infection. a total of 5182 animals (from a stratified systematic random sample of 273 herds) were tested with a commercial c. burnetii phase 2 igg elisa. a total of 6.2% of animals and 48.4% of herds tested positively. resu ...201019480726
q fever cholecystitis in an unvaccinated butcher diagnosed by gallbladder polymerase chain reaction.acalculous cholecystitis has been described in association with q fever. we describe the first known case of q fever cholecystitis diagnosed by polymerase chain reaction on gallbladder tissue. the presence of coxiella burnetii genome in the diseased gallbladder tissue argues for direct involvement of the organism in the pathogenesis of q fever cholecystitis.201019725764
histopathology of a granulomatous lobular panniculitis in acute q fever: a case report.q fever is a zoonotic infection caused by coxiella burnetii. two forms of the disease have been described: an acute form with pneumonia, hepatitis or a flu-like syndrome; and a chronic form in which endocarditis is the most frequent clinical expression. we report a 77 year old male with fever and an erythematous nodule on the right leg. biopsy revealed a granulomatous lobular panniculitis with some granulomas rimmed by an eosinophilic material, giving a "doughnut" or "fibrin-ring" appearance. q ...201019751228
coxiella burnetii modulates beclin 1 and bcl-2, preventing host cell apoptosis to generate a persistent bacterial infection.coxiella burnetii is the etiological agent of the human disease, q fever, and is an obligate intracellular bacterium that invades and multiplies in a vacuole with lysosomal characteristics. we have previously shown that coxiella interacts with the autophagic pathway as a strategy for its survival and replication. in addition, recent studies have shown that coxiella exerts anti-apoptotic activity to maintain the host cell viability, thus generating a persistent infection. in the present report, w ...201019798108
[severe cholestatic hepatitis due to q fever: report of a case].severe cholestatic hepatitis due to coxiella burnetii is a rare form of clinical presentation of acute q fever that is only occasionally detected in association with this infectious disease. we report a case of severe cholestatic hepatitis due to acute q fever, with clinical signs of hepatic insufficiency, marked cholestasis, and renal insufficiency. the results of the initial serologic study using a complement fixation test were negative on two occasions, and transjugular biopsy and serological ...201019819043
q fever.q fever is a zoonotic disease caused by the ubiquitous pathogen coxiella burnetii responsible for acute and chronic clinical manifestations. farm animals and pets are the main reservoirs of infection, and transmission to human beings is mainly accomplished through inhalation of contaminated aerosols. this illness is associated with a wide clinical spectrum, from asymptomatic or mildly symptomatic seroconversion to fatal disease. in humans q fever can manifest as an acute disease (mainly as a sel ...201019875249
investigation of a q fever outbreak in a scottish co-located slaughterhouse and cutting plant.outbreaks of q fever are rare in the uk. in 2006, the largest outbreak of q fever in scotland occurred at a co-located slaughterhouse and cutting plant with 110 cases. preliminary investigations pointed to the sheep lairage being the potential source of exposure to the infective agent. a retrospective cohort study was carried out among workers along with environmental sampling to guide public health interventions. a total of 179 individuals were interviewed of whom 66 (37%) were migrant workers. ...201019912614
microbial infections in eight genomic subtypes of chronic fatigue syndrome/myalgic encephalomyelitis.the authors have previously reported genomic subtypes of chronic fatigue syndrome/myalgic encephalomyelitis (cfs/me) based on expression of 88 human genes.201019955554
identification of risk factors of coxiella burnetii (q fever) infection in veterinary-associated populations in southern taiwan.the first case of q fever in taiwan was reported in 1993. the disease is considered to be emerging in taiwan, but the route of transmission has remained unclear. the annual number of confirmed q fever cases has been increasing up to more than 100 cases since 2005, comparing with less than 30 before 2003. the purpose of this study was to determine the seroprevalence and risk factors of coxiella burnetii infection in veterinary-associated populations in southern taiwan. a total of 228 serum sample ...201019968850
detection of coxiella burnetii by nested pcr in bulk milk samples from dairy bovine, ovine, and caprine herds in iran.the epidemiology of q-fever in iran is essentially unknown. this study was conducted to determine the prevalence rate of coxiella burnetii in bulk milk samples from dairy bovine, ovine, and caprine herds in chaharmahal va bakhtiari province, iran. in this study, 376 bulk milk samples from 79 dairy bovine, ovine, and caprine herds were tested for c. burnetii using a nested pcr assay. the animals whose milk samples collected for this study were clinically healthy. in total, 13 of 210 (6.2%) bovine ...201019968851
seroprevalence of neospora caninum antibodies and its consequences for reproductive parameters in dairy cows from dakar-senegal, west africa.the aim of this study was to determine the seroprevalence of neospora caninum antibodies and its effects on reproductive parameters in cows in intensive dairy herds in dakar. randomised blood samples were taken for serology from 196 cows in four herds with a history of sporadic abortion. all of the sera were assayed for antibodies against n. caninum, candida guillermondii, coxiella burnetii, and chlamydophila sp. the associations between serostatus and reproductive parameters (abortion, number o ...201019997972
seroprevalence of q fever (coxiellosis) in sheep from the southern marmara region, turkey.little information is available in turkey on q fever, a zoonose caused by coxiella burnetii and transmitted from domestic ruminants. this study aimed at investigating the seroprevalence in sheep flocks from three provinces (bursa, balikesir and canakkale). serosurvey was undertaken on 42 flocks, which were categorised by sizes. sera were collected randomly from specific age groups within the young population. chekit q-fever elisa kit was used to identify the infection in sheep. the results showe ...201018848356
prevalence of q fever in domestic animals with reproductive disorders.the occurrence of coxiella burnetii in animals with reproductive disorders was studied. a total of 920 samples (genital and faecal swabs, milk, urine and serum) were collected from cows (88), buffaloes (33), sheep (43) and goats (53) with a history of reproductive disorders and screened for c. burnetii by a pcr assay targeting the repetitive transposon-like region of c. burnetii (trans-pcr), real-time pcr, indirect immunofluorescence assay (ifa), enzyme-linked immunosorbent assay (elisa) and iso ...201019101035
systemic immune presentations of coxiella burnetii infection (q fever).q fever is a worldwide zoonosis caused by coxiella burnetii. its presentation can be atypical, delaying and complicating the diagnosis. we report 7 cases of q fever mimicking vasculitis, systemic inflammatory disease, or auto-immune disorder.201019110298
kinetics of coxiella burnetii excretion in a commercial dairy sheep flock after treatment with oxytetracycline.the kinetics of coxiella burnetii excretion were studied in dairy sheep using a flock that had a previous history of abortion and a positive polymerase chain reaction (pcr) test for c. burnetii in milk from the bulk-tank. an elisa used to test sera antibodies revealed a high within-flock seroprevalence (54%). fifty individual milk samples analysed by pcr showed a high number of milk shedders in the flock (38%). in the following breeding cycle, 75% of the animals in the flock were double treated ...201019269867
prevalence of coxiella burnetii and rickettsia spp. in ticks and rodents in southern germany.coxiella burnetii, the causative agent of q fever, and rickettsia spp. are bacterial pathogens that can be transmitted by ticks of the genus dermacentor (i.e., dermacentor marginatus and d. reticulatus). in germany, the occurrence of these ticks is currently limited to few areas. however, due to increasing temperatures, these vectors will likely extend their distribution in the future, and c. burnetii and rickettsia spp. might spread with them. to assess the prospective risk of human infections ...201021771522
surveillance of wildlife zoonotic diseases in the balkans region.the countries of the balkan peninsula have become the region with frequent outbreaks of the emerging and re-emerging diseases during the last decade of the 20th and the first decade of the 21st century. the majority of outbreaks were wildlife zoonotic, and vector-borne diseases, such as brucellosis, leptospirosis, listeriosis, tularemia, q-fever, lyme disease, anthrax, rabies, viral hemorrhagic fevers, sandfly fever, tick-borne encephalitis and leishmainiasis. epidemiological factors determined ...201021258303
detection of coxiella burnetii in placenta and abortion samples from british ruminants using real-time pcr.a real-time pcr was developed to detect coxiella burnetii (the cause of q fever) in ruminant placentas and aborted fetuses. primer and probe sets previously developed for human tissue studies were used to target the insertion sequence is1111 gene for c burnetii. the assay was highly sensitive, with a limit of detection of 10 copies of template, theoretically equating to a single bacterium, and did not cross-react with a panel of other bacteria. to determine sensitivity on field samples submitted ...201021262712
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