Publications

human very low density lipoproteins: loss of electrophoretic mobility on enzymatic removal of sialic acid residues.	incubation of very low density lipoproteins (vldl) from three hyperlipoproteinemic patients with neuraminidase resulted in a progressive liberation of sialic acid residues. after the original sialic content had been reduced by approx. 75% a lipoprotein fraction having zero electrophoretic mobility on paper appeared. this fraction increased until, after the removal of all the sialic acid residues, the mobility of the entire vldl fraction was reduced to zero.	1975	165020
immune response of cattle to intranasally or parenterally administered parainfluenza type 3 virus vaccines.	the principal significance of pi-3 virus in the bovine respiratory disease complex would seem to be its role in predisposing the respiratory tract to bacterial infection by (5) disrupting the integrity of the superficial mucosa and (3) producing a more favourable milieu for bacterial growth. attention has therefore been given to establishing local immunity to preclude infection of superficial cells of the mucosa. criteria used to evaluate the degree of such immunity are (5) presence of specific ...	1975	165124
local and systemic response after simultaneous intranasal inoculation of temperature-sensitive mutants of parainfluenza 3, ibr and bovine adenovirus 3.	triple seronegative calves were exposed by the nasal route to three (ts) mutants of bovine respiratory viruses (pi3, ibr, adeno3). after a single exposure, they responded with significant levels of serum antibodies to the three viruses. nasal antibodies were demonstrated for pi3 and adenovirus antigens. the failure to demonstrate nasal antibodies to ibr may be due to lack of sensitivity of the procedure used. when reexposed six weeks later, calves had sharp increases in levels of serum antibodie ...	1975	165126
organ cultures of rat and hamster colon.	human fibrin foam as a matrix for three-dimensional organ cultures was successfully employed for the cultivation of newborn rat and hamster colon tissue. colonic tissue was maintained under different oxygen atmospheres and with various synthetic media. explants of rat and hamster colon, maintained in leibovitz's l-15 medium buffered with hepes in the presence of 5% co2 and air, retained their normal columnar epithelial architecture for 24 days in culture. outgrowth of epithelial cells from colon ...	1975	165141
non-producer human cells induced by murine sarcoma virus.	non-produces (np) human cells were isolated from transformed foci induced by the kirsten mouse sarcoma virus. these morphologically altered np cells produced neither infectious virus nor complement-fixing antigens of the murine sarcoma-leukemia virus complex. however, the sarcoma virus genome could be rescued from these nf cells by co-cultivation with cells carrying "helper" kirsten mouse leukemia virus or woolly monkey leukemia virus. the possible usefulness of these cells in efforts designed t ...	1975	165148
hydrophobic interactions of the apo-gln-i polypeptide component of human high density serum lipoprotein.	apo-gln-i, the major polypeptide component of human serum high density lipoprotein, has four noninteracting hydrophobic sites which associate with alkanes, anionic detergents, and cationic detergents. hexane and octane bind to these sites with association constants of 6.8 times 10-2 and 1.8 times 10-4 liters/mol, respectively, and compete with the anionic detergent, sodium dodecyl sulfate (c12oso3-minus), at low detergent ligand binding ratios (i.e. smaller than or equal to 1.0 mol of c12oso3-mi ...	1975	165177
enterotoxins of escherichia coli and vibriocholerae: tools for the molecular biologist.	with the independent discovery in several different laboratories that vibrio cholerae and heat-labile escherichia coli enterotoxins activated an enzyme (adenylate cyclase) in small intestinal epithealial cells to cause an enhanced intestinal secretion mediated by cyclic adenosine 3',5'-monophosphate (cyclic amp), a molecular mechanism was provided for these disease states. as agents that also elevate intracellular concentrations of cyclic amp in virtually every mammalian tissue tested, the enter ...	1975	165247
long-term primary culture of mouse mammary tumor cells: production of virus.	long-term primary cultures of mouse mammary tumor cells proved an excellent source of mouse mammary tumor virus (mmtv). virus purified from these primary cultures had the same morphologic biochemical, immunologic, and biologic characteristics as mmtv. quantitation of mmtv-protein equivalents released into the medium was measured by the radioimmunoassay for mmtv. peak production levels were 20-40 mug mmtv protien equivalents/75-cm-2 flask/24 hours. these cultures produced mmtv for as long as 90 ...	1975	165313
[biochemical characteristics of nucleic acid decomposing enzymes of the epidermis].		1975	165330
assessment of in vivo adrenocorticotropic hormone treatment on adrenal mitochondrial functions in vitro.		1975	165376
rhythmic and phytochrome-regulated changes in transmembrane potential in samanea pulvini.		1975	165432
inhibition of fc receptors on a murine lymphoid cell line by cholera exotoxin.		1975	165433
letter: petechiae: due to adenovirus type 7 infection or to acetylsalicylic acid therapy?		1975	165451
a simple radioimmunoassay for 3',5', cyclic adenosine monophosphate.		1975	165745
actions of a new muscle relaxant (ah8165) on neuromuscular transmission.	the effects of a new muscle relaxant, ah8165, on miniature endplate potential (mepp) amplitude and frequency, endplate sensitivity to acetylcholine, and muscle twitch tension were studied in vitro in the frog sartorius muscle. nerve terminal effects were studied in vivo in the cat soleus muscle and its ventral root fibers. ah8165 stimulates the nerve terminal, as evidenced by increased mepp frequency and the appearance of post-drug repetitive activity. in the same concentration range at which me ...	1975	165756
the isolation of thymopoietin (thymin).	the isolation from bovine thymus of two closely related polypeptides, thymopoietin i and ii, is described. these are considered to be thymic hormones, which physiologically induce the differentation of prothymocytes to thymocytes within the thymus. ths isolation of the thymopoietins was monitored not by their differentiative effects, but by a presumably secondary effect on neuromuscular transmission. this was discerned in experimental studies related to the human disease myasthenia gravis in whi ...	1975	165765
respiratory virus infections in children hospitalized in montreal, 1968 - 1972.		1975	165872
biochemical and morphological changes in the subcellular fractions during myelination of rat brain.		1975	165992
in-vitro activity of methronidazole on the cysts of entamoeba histolytica.		1975	166039
cyclic amp metabolism and nucleic acid content in the lymphocytes of the thymus, spleen, and lymph nodes of protein-deficient rats.	the concentration of cyclic amp (camp) and its metabolites (5'-amp and adenosine) as well as the adenyl cyclase, camp phosphodiesterase, and 5'-nucleotidase activities were determined in lymphocytes of thymus, spleen, and lymph nodes of control and protein-deficient rats. the values of these parameters, when expressed as per milligran dna and as per 10-8 cells, but not always when expressed as per milligran protein, were much lower in the thymus as compared with the spleen and the lymph nodes in ...	1975	166150
dexamethasone-binding receptor in bovine pituitary cytosol.		1975	166255
control of arrhythmias following ligation of the circumflex artery.	in experimental work, three groups of 10 dogs each were subjected to ligation of the circumflex coronary artery. one group was given prophylactic medication with procainamide and another group was allowed a 2 hour interval between thoracotomy and ligation. the effects of these measures on the incidence of ventricular arrhythmias during the 30 minutes immediately after coronary artery occlusion were then compared. the two groups of animals on which these preventive measures were tested both showe ...	1975	166259
involvement of nicotinamide adenine dinucleotide in the action of cholera toxin in vitro.	nad is a necessary cofactor for the activation of adenylate cyclase (atp pyrophosphate-lyase (cyclizing), ec 4.6.1.1) by cholera toxin. lysates of certain types of cell that hydrolyze their endogenous store of nad after cell disruption respond poorly or not at all to cholera toxin. lysates of pigeon erythrocytes, which lack enzymes that degrade nad, provide a convenient and reproducible system for assaying the activity of cholera toxin in vitro and allow investigation of the mechanism of action ...	1975	166378
isolation of a glycogen synthase i kinase that is independent of adenosine 3':5'-monophosphate.	three protein kinases (atp:protein phosphotransferase, ec 2.7.1.37) were detected when the soluble fraction of rabbit kidney medulla was chromatographed on deae-cellulose with a linear nac1 gradient. the first two kinases eluted (peak 1 and peak ii) were cyclic-amp-dependent, wheras peak iii was cyclic-amp-independent. a procedure was developed to separate the catalytic subunit of peak ii cyclic-amp-dependent protein kinase (representing the bulk of the histone kinase activity) from peak iii pro ...	1975	166380
[rna involvement in dna replication (author's transl)].		1975	166414
pleiotropic effects of a dna adenine methylation mutation (dam-3) in escherichia coli k12.	the dam-3 mutation results in a five-fold reduction in the number of 6-methyl-adenine (6-mea) residues in the dna of e. coli k12 or phage lambda. the dna of phage fd appears to be devoid of 6-mea when propagated on dam-3 bacteria. the phenotypic differences between dam-3 and dam+ bacteria include: (i) increased free phage in lysogenic dam-3 cultures, (2) increased sensitivity to methyl methanesulfonate (mms), (3) inviability of dam-3 lex-i strains, (4) lower molecular weight of dna in dam-3 bact ...	1975	167279
wound infection during the yom kippur war: observations concerning antibiotic prophylaxis and therapy.	eighty-eight episodes of wound associated infection were identified among 624 consecutively admitted battlefield casualties. ninety-one per cent of infections occurred during the administration of antibiotic therapy or prophylaxis and 65% were associated with the use of multiple antibacterial agents. gram negative bacillary and mixed microbial infection predominated and were found to increase in relative incidence after the second day of hospitalization. appropriate therapy, based on disc sensit ...	1975	167666
stimulation of late interphase dictyostelium discoideum amoebae with an external cyclic amp signal.	the microelectrode system described in the accompanying paper was used to investigate properties of fields of dictyostelium discoideum amoebae in late interphase. cells in the fields were competent to respond chemotactically to, and to relay, a c-amp signal, but not to produce an aggregative signal autonomously. the experimental results are generally consistent with c-amp being the sole compound required for chemotaxis and signal relaying. a periodic signal from the microelectrode can initiate a ...	1975	167879
adrenal cells in tissue culture the effects of choleragen and acth on steroid and cyclic-amp metabolism.	primary cultures of mouse adrenocortical tumors provide a sensitive system for investigating the effects of the enterotoxin of the v. cholerae (choleragen) on cyclic-amp metabolism in the intact cell. like acth, the toxin stimulates the synthesis and release of steroids from these cells but its mode of action differs from that of acth. the steroidogenic response to acth is immediate and of limited duration. the initial rate of steroidogenesis is the highest. in contrast, the steroidogenic respon ...	1975	168056
the action of vibrio cholerae and corynebacterium diphtheriae neuraminidases on the sendai virus receptor of human erythrocytes.	studies of the sendai virus haemagglutinin receptor on the human erythrocyte surface have confirmed that it involves 2 leads to 3 linked sialic acid. because the primary specificity of vibrio cholerae neuraminidase is for this linkage, it is able to compete with the virus for the receptor, to which it adsorbs strongly at low temperatures. corynebacterium diphtheriae neuraminidase, whose principal specificity is for a sialic acid linkage other than 2 leads to 3, does not easily remove sendai viru ...	1975	168311
antibody levels to parainfluenza, rubella, measles, and influenza a virus in children with polymyositis.	the cf and hi antibody titers to rubella and measles viruses, the cf titers to influenxa a, and the hi titers to parainfluenza 1, 2, and 3 were carried out on the sera of 20 patients with childhood polymyositis and their matched controls. the titers for measles, parainfluenxa 1, and influenza a were slightly higher for patients than for controls. the control group had antibody titers to rubella and parainfluenza 2 and 3 higher than or similar to those of patients. strong patterns or significant ...	1975	168906
characterization of cloned evolutionary variants of simian virus 40.		1975	169050
polyoma virus--a study of wild-type, mutant and defective dnas.		1975	169088
fetal calf serum alters cyclic adenosine 3',5'-monophosphate's effect on heme synthesis in vitro.	an investigation of the effect of camp on heme synthesis of rat bone marrow cells revealed that at 10(-2) m this cyclic nucleotide inhibits heme synthesis and that optimum stimulation occurs at 10(-4) m. some unidentified constituent of fetal calf serum in the culture medium modifies the direction and degree of camp's effect.	1975	169145
the differential effect of cholera toxin on the lymphocyte stimulation induced by various mitogens.	balb/c spleen cells (5 x 10(6)) were cultured in 1 ml of serum-free rpmi 1640 medium for 3 days in order to examine the effect of cholera enterotoxin (cn) and its spontaneously formed toxoid (cd) on lymphocyte stimulation. stimulation was assessed after addition of [3h] thymidine for the last 16 hours of culture. one microgram of cn per culture markedly reduced the baseline of [3h] thymidine incorporation and the stimulation due to phytohaemagglutinin (pha), concanavalin a (con a) and bacterial ...	1975	169198
effect of klebsiella pneumoniae enterotoxin on intestinal transport in the rat.	the effects on intestinal transport of either a semipurified preparation of enterotoxin elaborated by klebsiella pneumoniae or similaryly prepared control material were tested by marker perfusion studies in the small intestine of rats. at a concentration of 2 mg/ml, the enterotoxin produced net secretion of water, na, and cl in both jejunal and ileal segments; hco3 transport was not affected. net secretion was evident within 30 min after intorduction of the toxin and was maximal after 90 min. th ...	1975	169297
recent developments in immunization against diarrheal diseases.		1975	169592
functional consequences of modifying highly reactive arginyl residues of fructose 1,6-bisphosphatase. loss of monovalent cation activation.	modification of pig kidney fructose 1,6-bisphosphatase with 2,3-butanedione (in the presence of amp) results in the loss of activation of the enzyme by monovalent cations. under these conditions about 8 arginyl residues per mole of enzyme were modified. no other residues were modified. no loss of monovalent cation activation occurs when modification with 2,3-butanedione is carried out in the presence of amp plus the substrate fructose 1,6-bisphosphate and 3.2 less arginyl residues were modified. ...	1975	169892
2-chloroadenosine-dependent elevation of adenosine 3',5'-cyclic monophosphate levels in rat caudate nucleus slices.		1975	169954
ultrastructure of human bronchiolo-alveolar cell carcinoma.	ultrastructural features were correlated with a series of special staining reactions in eight cases of bronchiolo-alveolar carcinoma. ultrastructural, all tumors were similarly composed of large cells with abundant cytoplasm and small nuclei in close contact with each other. straight membranes or complex interdigitations occurred within adjacent tumor cells, attached to each other by scattered desmosomes. microvilli or cilia abutted from free surfaces of the cells, and were noted in different st ...	1975	169987
genetic control of oncogenesis by murine sarcoma virus moloney pseudotype. ii. a dominant epistatic susceptibility gene.	we have shown in the preceding paper that akr mice are highly resistant to m-msv tumor development, and that resistance is transmitted as a dominant character. in the present studies the tumor-response pattern of f1 hybrids between resistant akr and susceptible strains (c57bl/6, balb/c and b10br) following injection with moloney mouse sarcoma virus (m-msv) resembles that of the non-akr parent. segregation is observed in first backcross (bc1) and f2 mice, and the segregation ratios up to bc3 mice ...	1975	170220
mode of action of sodium nitroprusside on vascular smooth muscle.	1. sodium nitroprusside is a potent relaxant of smooth muscles with a predominantly tonic response, e.g. rat aorta contracted by noradrenaline, angiotensin ii, phe2-lys8-vasopressin, bac1(2), or kc1, and guinea-pig tracheal smooth muscle contracted by carbachol. 2. smooth muscle preparations from the splanchnic region and with varying degrees of phasic contractility are less sensitive and develop tachyphylaxis (portal vein, duodenum of the rat) or are unresponsive to sodium nitroprusside (vas de ...	1975	170545
homology and relationship between the genomes of papovaviruses, bk virus and simian virus 40.	a number of hybridization techniques have been used to assess the homology between the genomes of bk virus (bkv) and simian virus 40 (sv40). a noncontiguous set of homologous sequences has been localized primarily within the late region of the sv40 genome, and these sequences presumably account for the cross-reaction between v-antigens of the two viruses. the reason for the relatively strong crossreaction between sv40 and bkv t-antigens is still unclear. the sequence homology and similarity in g ...	1975	170606
[ultrastructure of human hepatocytes in early prenatal ontogenesis].	the structure of hepatic cells was studied in 40 human embryos and fetuses from 4 to 16 weeks of intrauterine development. depending on the differentiation degree of organells and their amount in the hepatic cell of the embryo and fetus three types of hepatic cells are distinguished: slightly differentiated, transitional and differentiated cells. in the course of early prenatal ontogenesis the amount of slightly differentiated hepatic cells diminishes and the amount of transitional and different ...	1975	170891
on the mechanism of action of cholera toxin on isolated rat adrenocortical cells. comparison with the effects of adrenocorticotropin on steroidogenesis and cyclic amp output.	the effects of cholera toxin on isolated rat adrenocortical cells have been investigated. both steroid and cyclic amp output from adrenal cells were increased by the toxin in a dose dependent fashion. the concentration of toxin for half maximal stimulation for both of these responses was about 40 ng/ml. maximal steroidogenesis and cyclic amp output was obtained with similar concentrations of the toxin. a correlation was observed between the low amounts of cyclic amp produced in response to all d ...	1975	170975
choleragen stimulates steroidogenesis and adenylate cyclase in cells lacking functional hormone receptors.	choleragen stimulates steroid secretion and adenylate cyclase in three cell lines, adrenal tumor line (y-1), a corticotropin-resistant mutant derived from y-1 called os-3, and a receptor-deficient leydig tumor line (i-10). sensitivity for half-maximal stimulation varies from 3 to 36 pm choleragen, the i-10 line being the most sensitive. latency before the onset of steroidogenesis is longer in os-3 and i-10 cells than in the y-1 line. in both os-3 and i-10 cells choleragen stimulates adenylate cy ...	1975	172154
endotoxic lipopolysaccharides stimulate steroidogenesis and adenylate cyclase in adrenal tumor cells.	lipopolysaccharides (endotoxins) from escherichia coli, serratia marcesens and salmonella typhosa stimulated steroid production in y-1 adrenal tumor cells in culture with a latent period of 3-4 h. lipid a, derived from escherichia coli lipopolysaccharide, also stimulated steroidogenesis. lipopolysaccharides and lipid a also stimulate adenylate cyclase activity and cause rounding of the cells. in contrast, lipopolysaccharides do not stimulate steroidogenesis in receptor-deficient adrenal tumor ce ...	1975	172155
proceedings: cyclic amp in hela cells stimulated with cholera enterotoxin and methylxanthines.		1975	172178
naturally occurring autoantibodies against hidden cellular antigens.		1975	172441
mechanism of activation of adenylate cyclase by vibrio cholerae enterotoxin. relations to the mode of activation by hormones.	the influence of vibrio cholerae enterotoxin (choleragen) on the response of adenylate cyclase to hormones and gtp, and on the binding of 125i-labeled glucagon to membranes, has been examined primarily in rat adipocytes, but also in guinea pig ileal mucosa and rat liver. incubation of fat cells with choleragen converts adenylate cyclase to a gtp-responsive state; (-)-isoproterenol has a similar effect when added directly to membranes. choleragen also increases by two- to fivefold the apparent af ...	1975	172636
sphingomyelinases in human tissues. iii. expression of niemann-pick disease in cultured skin fibroblasts.	sphingomyelinase was effectively extracted cultured fibroblasts homogenized in 1% glycine. the average specific activity was 24.6 nmol substrate hydrolyzed per hr per mg protein. cultured cells from two cases of niemann-pick disease type a and one case of type b had markedly reduced enzyme activity, whereas, in type e cells, total activity was twice normal. sphingomyelinase was resolved by isoelectric focusing into three peaks of activity (i-ii), where i and ii were the major forms. species i ha ...	1975	172849
mechanism of action of pituitary growth hormone.		1975	172992
neuronal control of neurochemical processes in the basal ganglia.		1975	173060
pathways of lipoprotein metabolism: integration of structure, function and metabolism.		1975	173155
prostaglandin e in cholera toxin-induced intestinal secretion. lack of an intermediary role.	prostaglandin e1 (pge1) and cholera enterotoxin stimulate small-intestine mucosal adenylate cyclase and intestinal secretion of water and electrolytes. the previous suggestion that pge may mediate cholera-toxin effects was explored in these studies. closed rabbit jejunal loops were injected in vivo with cholera toxin and compared to similar loops in the same animal injected with buffer. loop mucosal homogenates and intestinal secretions were analyzed by radioimmunoassay for camp and pge concentr ...	1975	173182
an investigation of the involvement of adenosine 3':5'-cyclic monophosphate in steroidogenesis by using isolated adrenal cell column perfusion.	the involvement of cyclic amp in corticosteroidogenesis was investigated by using isolated adrenal cell column perfusion. steroids were produced in response to 0.5, 1.0 and 5.0 mg of cyclic amp/ml. analysis of the shape of the response curves indicated an inverse relationship between rate of onset of steroid production and dose. a further increase in steroid production during the washout period after the 5 mg/ml dose was considered to indicate an intracellular inhibitory effect of cyclic amp. re ...	1975	173285
the photogeneration of superoxide by isolated photoreaction center from rhodospirillum rubrum.		1975	173311
antibody-dependent lymphocyte mediated cytotoxicity mechanism and modulation by cyclic nucleotides.		1975	173470
variations of cyclic nucleotide monophosphate levels during spontaneous uterine contractions.	oscillations on the concentrations of cyclic nucliotides occurred during spontaneous rhythmic contractions in rat uterus. the levels of cyclic gmp and cyclic amp were highest at the beginning of the contraction respectively the relaxation.	1975	173575
[studies on the physiopathology of cholera in bari].	during a small epidemic of cholera in south italy in summer of 1973, the infection showed some peculiarities and differences in comparison with cholera classic picture of asiatic areas. in this paper epidemiological, pathogenetic, clinical and biochemical data of patients admitted to the institute of infectious diseases of bari are reported. the age of the majority of patients (over 50) is an important factor for understanding cause of infection; but also preexistent basal diseases are responsib ...	1975	180912
[localization of genome rna synthesis in sendai virus].	localization of sendai virus 50s rna in ehrlich ascitic carcinoma cells was studied. at 48 hours postinfection virus-specific 50s rna was found in the nucleus after 30 min exposure to 3h-uridine, and its amount increased after 1-hour exposure to the precursor reaching 18% of the total nucleus virus-specific rna. after 2-hour or longer exposure to the precursor the amount of 50s rna in the nuclei decreased considerably and in the cytoplasm in this gradient region a considerable radioactivity appe ...	1975	179216
[health aspects of the study of bdellovibrio bacteriovorus as a natural factor in the self-purification of reservoirs].		1975	179245
[the biological role of estriol].	it was demonstrated on the uteri of women and guinea pigs that estriol (in vitro) possessed a marked affinity to the estradiol-binding system of human and guinea pig uteri; the activity of steroid-receptor interaction of estriol in vitro constituted 9.4% for guinea pigs and 17% for man in relation to the estradiol activity. administration of estriol to guinea pigs in vivo in a dose of 0.25-0.5 mg led to a sharp reduction of the estradiol-binding capacity of the receptor system of the uterus. it ...	1975	179639
therapy in an intracerebral murine glioma model, using bacillus calmette-guérin, neuraminidase-treated tumor cells, and 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea.	the s.c.-propagated murine glioma, gl-26, was established in tissue culture. the tissue culture line, with a doubling time of 36 hr, was used as the common source for all tumor cells. suspensions of the tumor cells were transplanted intracerebrally in mice to produce an anaplastic ependymoblastoma. in vitro 51-cr cytotoxicity assays did not detect any cellular immunity against gl-26 tumor cells in animals bearing either s.c. or i.c. tumors, indicating that the tumor itself is not highly immunoge ...	1975	234790
microbial interference with host defence mechanisms.		1975	235083
natural antibodies in healthy adults.	the serum of 100 adults living in budapest was examined for isohaemagglutinin titre with haemaglutination, for staphylococcal-antitoxin titre with haemolysis inhibition and for bacterial antibody titre against 17 different groups of bacteria with passive haemagglutination. antibody levels in males, except for certain bacterial antibodies, were somewhat lower than in females. the antibody titres, especially in men, decreased gradually from 20 to 50 years of age and were usually lower in rh negati ...	1975	235827
resolution, purification and characterization of the orthophosphate releasing activities from fracture callus calcifying cartilage.	callus calcifying cartilage alkaline phosphatase was resolved by deae-cellulose column chromatography into two distinct phsophatase activities. the phosphatase activity which was eluted first from the column, (phosphatase i), was active towards a variety of phosphate esters, sodium pyrophosphatase and several linear polyphosphates, while the second phosphatase activity , (phosphatase ii), was active toward simple phosphate esters but not towards sodium pyrophosphate and linear oligo or polyphosp ...	1975	238599
purification and characterization of two activities of the intracellular dextransucrase from leuconostoc mesenteroides nrrl b-1299.	dextransucrase (sucrose: 1,6-alpha-d-glucan 6-alpha-glucosyltransferase ec 2.4.1.5) activity of leuconostoc mesenteroides nrrl b-1299 cells has been purified by adsorption on hydroxyapatite, followed by chromatographies on deae-cellulose and deae-sephadex. the enzyme activity was readily separated into two principal forms of the enzyme, i and ii, by deae-cellulose chromatography. both enzymes i and ii were purified to an electrophoretically homogeneous state in which the relative enzyme activiti ...	1975	238637
intestinal villus and crypt cell responses to cholera toxin.	adenylate cyclase activity was measured in rat small intestinal villus and crypt cells after in vivo and in vitro exposure to cholera toxin. the increase in intestinal adenylate cyclase induced by cholera toxin in vivo appeared to be largely confined to the villus cell with the largest increase observed for upper villus cells. crypt cell adenylate cyclase was not responsive to cholera toxin. no response could be demonstrated for isolated villus or crypt cells incubated with cholera toxin in vitr ...	1975	238900
methods for analyzing percentage lipid of ground beef and beef-soy blends.		1975	239047
factors influencing the excretion and relative physiological availability of pethidine in man.	factors influencing the urinary excretion of pethidine, norpethidine and pethidine n-oxide have been examined. the proportion of a dose of pethidine excreted unchanged or as norpethidine depends on the urinary ph and the route of administration. older people appear to metabolize more of the drug and therefore excrete less unchanged pethidine. the rate of excretion of pethidine in acid urine is directly proportional to the plasma pethidine concentration and under these conditions the relative phy ...	1975	239115
the role of dopamine and noradrenaline in temperature control of normal and reserpine-pretreated mice.	drugs with the common property of stimulating dopamine receptors, have been tested for their effects on core temperature in control and reserpine-pretreated mice. apomorphine, amantadine, amphetamine, l-dopa and atropine all produced a fall in mouse oesophageal temperature, their efficacy correlating with their ability to activate central dopamine receptors. amphetamine and l-dopa had a biphasic effect the initial fall being followed by a rise. in reserpine-pretreated mice only amphetamine, apom ...	1975	239116
cholinergic modification of abnormal involuntary movements induced in the guinea-pig by intrastriatal dopamine.		1975	239121
the alpha-adrenoceptor blocking effects of a new benzodioxane.		1975	239130
[clinical effects of lividomycin on respiratory tract infections, primarily wet cases of bronchiectasis (author's transl)].	patients with various respiratory infections, primarily wet cases of bronchiectasis, were treated with lividomycin (lvdm) at a dose of 1 g/day for about a week. the results obtained are as follows: 1. of the 15 patients with refractory bronchiectasis who had previously been treated with various other antibiotics, 11 patients (73.3%) responded to lvdm. although there was no patient who responded to lvdm excellently, good cures were achieved in 5 pateints and fair cures in 6 patients. 2. in bronc ...	1975	239263
glutathione conjugates as metabolites of benz[a]anthracene.	1. [3h]benz[a]anthracene is converted into water-soluble metabolites by microsomal plus soluble fractions of rat-liver in the presence of nadph and glutathione. chromatography on sephadex g25 gave four radioactive peaks; the first contained hydrocarbon or hydrocarbon derivatives bound to soluble protein while the other three peaks contained glutathione conjugates of hydrocarbon metabolites. 2. conjugates formed when either of the benz[a]anthracene metabolites, 5,6-dihydro-5,6-dihydroxybenz[a]ant ...	1975	239491
boric acid tolerant vibrio cholerae: biological and physical properties.	two boric acid tolerant variants were developed by passage in boric acid containing media from a mouse virulent parent vibrio cholerae strain. the variants were stable, exhibited increased acid resistance, were less virulent to mice, but protected against challenge with the mouse virulent parent strain.	1975	239521
new concepts in meat processing.		1975	239547
properties of the cholera phage pl 163/10.	vibrio cholerae phage pl 163/10, belonging to mukherjee's group i, gave clear plaques with surrounding halos of overall diameters varying between 1 to 4 mm when plated on a lawn of host v. cholerae ogawa 154. it was fairly stable in the ph range 6-11. its thermal inactivation was characterised by half lives of 39, 12, 4.5 and 1.0 minutes at 55, 60, 65 and 70 degree c respectively. the thermodynamic parameters deltah, deltaf and deltas were determined at these temperatures. the phange was resista ...	1975	239574
the "otitis-prone" condition.	we studied the incidence of exudative otitis media in 488 patients followed up from birth. forty-nine percent (240) of the patients had their initial episode of otitis media in the first year of life and only 12% (56) in the second year of life. thereafter, the incidence decreased steadily. patients having six or more episodes of otitis media before age 6 were termed "otitis prone". fifty-seven such patients were observed and this condition was found to be significantly related to the onset of o ...	1975	239591
method for detecting small numbers of vibrio cholerae in very polluted substrates.	a method is presented for the indirect detection of vibrio cholerae by the multiplication of two specific bacteriophages: phih74/64 for el-tor vibrios, and phage group iv (mukerjee) for classical vibrios. the product to be examined is seeded in alkaline tryptone water for enrichment, as in the classical method, and is then incubated for 6 h at 37 c. thereafter, a loopful is transferred to each of two nutrient broth (ph 9) tubes. one of these receives a drop of phage phih74/64; the other receives ...	1975	239631
[characteristics of a mycobacterium mucosum culture oxidizing cholic acid].	some physiologo-morphological and biochemical characteristics were studied with the culture of mycobacterium mucosum 1210 which can assimilate cholic acid as a sole source of carbon. this culture is capable of spontaneous variability. four variants of the culture were obtained which possessed different rates of the transformation of cholic acid. the variant which formed slimy colonies of pale-yellow colour was the most active. some morphological modifications of the cells were investigated in th ...	1975	240106
[michaelis constants of neuraminidases of pathogenic and apathogenic microorganisms (author's transl)].	the km-values of neuraminidases from different pathogenic and apathogenic microorganisms have been determined on low and high molecular substrates. the substrate specificity and the affinity to the different types of substrates in relation to the pathogenicity of the microorganisms are discussed.	1975	241169
aryl hydrocarbon hydroxylase in liver nuclei of c3h/he and dba/2 mice.	aryl hydrocarbon hydroxylase activity in liver nuclei from c3h/he mice is apparently increased by the administration of 3-methylcholanthrene, but the enzyme activity from the dba/2 mice is not. on the other hand, by treatment of the mice with phenobarbital sodium, the increased activity in liver nuclei was observed in both strains of mice. there are approximately the same levels of the apparent km for benzo[alpha]pyrene in liver nuclei from both strains of mice even after treatment with 3-methyl ...	1975	241681
assay and properties of glutathione-s-benzo(a)pyrene-4,5-oxide transferase.		1975	242265
accelerated cell death produced by a cholera cytotoxin on isolated epithelial cells from rabbit ileum.	villus and crypt cells were removed from rabbit ileum by a modification of a method previously described. villus cells were isolated by vibration of everted bowel segments in citrate buffer, and crypt cells were removed by the expansion of these segments with air and simultaneous vibration in citrate buffer. several culture media were tested for the maintenance and/or culture of these cells. two defined media, eagle's minimum and medium 199, were unsatisfactory and led to rapid cell death. the p ...	1974	4549039
the transfer of endogenous and exogenous radioisotopically labelled nicotine to mainstream cigarette smoke and its absorption into the blood of anaesthetized cats.		1974	4521016
an outbreak of cholera in australia due to food served in flight on an international aircraft.	an outbreak of cholera occurred in november 1972 among passengers on an aircraft that had flown from london to sydney. the infection was confined to economy-class passengers and the available evidence indicates that it was due to a dish of hors d'oeuvres served on the aircraft between bahrain and singapore. although one person died, the infection was generally mild, and almost half of those infected were symptomless. there was a significant difference between the immunization status of persons w ...	1974	4526408
carbohydrate inhibition studies of the naturally occurring human antibody to neuraminidase-treated human lymphocytes.		1974	4528455
cholera toxin and cell growth: role of membrane gangliosides.	the binding of cholera toxin to three transformed mouse cell lines derived from the same parent strain, and the effects of the toxin on dna synthesis and adenylate cyclase activity, vary in parallel with the ganglioside composition of the cells. tal/n cells of early passage, which contain large quantities of gangliosides g(m3), g(m2), g(m1), and g(dla), as well as the glycosyltransferases necessary for the synthesis of these gangliosides, bind the most cholera toxin and are the most sensitive to ...	1974	4530298
affinity chromatography of a serotonin binding protein from rat brain.		1974	4531140
chromosome study and sex determination of chang human conjunctival cell line.		1974	4531478
neuraminidase and concanavalin a in the experimental immunotherapy of cancer.		1974	4536589
human t lymphocyte receptors for sheep erythrocytes: conditions for binding including inhibition by cytochalasin b.		1974	4544364
the cytotoxic reactivity and sialic acid content of human lymphoid cells.		1974	4545917
studies on toxinogenesis in vibrio cholerae. ii. an vitro test for enterotoxin production.	an elek test for enterotoxin-producing strains of vibrio cholerae is described. thirty-five out of 37 strains of classical v. cholerae produced positive reactions, but only 1 of 69 e1 tor vibrio strains was reactive. tox(-) mutants of v. cholerae were also unreactive in the elek test.	1974	4587384
effect of imidazole-4-carboxamide, 5-(3,3-dimethyl-1-triazeno) on immunity in patients with malignant melanoma.		1974	4588545
effect of imidazole-4-carboxamide, 5-(3,3-dimethyl-1-triazeno) on immunity in patients with malignant melanoma.		1974	4588545
persistence and dissemination in the community of r-factors of nosocomial origin.		1974	4590005
alterations in the immunogenicity and antigenicity of mammalian erythrocytes following treatment with neuraminidase.		1974	4591391