Publications

hepatic iodothyronine 5-deiodinase activity in rana catesbeiana tadpoles at different stages of the life cycle.	conversion of t4 to t3 cannot be demonstrated in vivo in rana catesbeiana tadpoles until just before metamorphic climax, suggesting that 5'-deiodinase (5'd) activity is not present until this time. in the present study the role of 5-deiodinase (5 d) systems in the metabolism of t4 and t3 in the developing tadpole was examined. 5 d activity capable of converting t3 to 3,3'-diiodothyronine, and t4 to rt3 was present in hepatic microsomes from pre- and prometamorphic tadpoles, but it declined to un ...	1987	3496211
peripheral blood lymphocyte response to recombinant and non-recombinant interleukin 2 in previously treated patients with hodgkin's disease, long-time off-therapy.	13 patients with hodgkin's disease (hd) previously treated, 9 of whom were long-time (more than 2 yr) off-therapy, were studied for peripheral blood lymphocyte response to interleukin 2 and for lymphocyte subpopulations by means of in vitro cultures and monoclonal antibodies. the aim of the study was to ascertain the role played by interleukin 2 in the impaired cell-mediated immunity of hd patients. the results show a response of peripheral blood mononuclear cells of hd patients to either the t ...	1987	3496236
autoradiographic localization of t4 antigen, the hiv receptor, in human brain.	using a novel autoradiographic technique and a monoclonal antibody to t4, the entry protein for the aids virus hiv, we have determined the distribution of t4 in selected areas of the human brain. t4 was found throughout the human brain in a pattern similar to that seen in squirrel monkey and rat, thus demonstrating a conservation of both the antigen and the antigen distribution. t4 was especially abundant in emotion-mediating areas, such as the hippocampus, which are typically enriched with neur ...	1987	3496314
visualization of the homologous pairing of dna catalyzed by the bacteriophage t4 uvsx protein.	the uvsx gene product is essential for dna repair and general recombination in t4 bacteriophage. the ability of uvsx protein to catalyze the homologous pairing of single-stranded dna (ssdna) with double-stranded dna (dsdna) in vitro was examined by electron microscopic (em), nitrocellulose filter binding, and gel electrophoretic methods. optimal joining was observed at ratios of uvsx protein:ssdna of 2 nucleotides/protein monomer. at this level, the ssdna was fully covered by uvsx protein as see ...	1987	3496334
phenotypic analyses of mononuclear cells recovered from healthy and diseased human periodontal tissues.	mononuclear cells were recovered from the gingival tissues of normal individuals and from patients with periodontal disease. lymphocyte phenotypic markers were identified by immunofluorescence after reaction with monoclonal antibodies to t-cell subset markers. the normal tissues exhibited t4/t8 ratios almost identical to those in the peripheral blood. the diseased tissue cell ratios were significantly reduced, in both the adult periodontitis and the juvenile periodontitis groups (p less than 0.0 ...	1987	3496355
interleukin 2 induces human acute lymphocytic leukemia cells to manifest lymphokine-activated-killer (lak) cytotoxicity.	lymphokine-activated killer cells (lak) were originally distinguished from natural killers (nk) and cytotoxic t lymphocytes. recently, however, il 2-activated nk cells were suggested as the major source of lak reactivity in human peripheral blood (pbl). because certain t cell acute lymphoblastic leukemia (t-all) cells are phenotypically similar to lak precursors, we have asked whether these leukemic cells can be induced toward lak-cytotoxicity and express nk reactivity before stimulation. five o ...	1987	3496395
nonrandom development of immunologic abnormalities after infection with human immunodeficiency virus: implications for immunologic classification of the disease.	blood specimens from 165 intravenous drug users who were seropositive for the human immunodeficiency virus (hiv), from 158 seropositive homosexual men with lymphadenopathy, and from 77 patients with acquired immunodeficiency syndrome (aids) were assessed immunologically. immunologic parameters were analyzed by the guttman scalogram technique to determine if immunologic abnormalities occurred in a nonrandom pattern. the following four patterns emerged: (i) seropositivity for hiv with no immunolog ...	1987	3496603
enkephalins and immunity. ii: in vivo modulation of cell-mediated immunity.	body, thymus, and spleen weights, and cellular makeup of lymphoid tissues of rat were not affected to a great extent by intraperitoneal injections of met-enkephalin, leu-enkephalin, or naloxone. however, enkephalins induced a diminution of peripheral blood leukocytes and lymphocytes. in addition, met-enkephalin depleted the population of t4 helper/inducer lymphocytes. on the other hand, there was an increase of blood leukocytes and lymphocytes in naloxone-treated animals. arthus and delayed skin ...	1987	3496823
heterogeneity of large granular lymphocyte proliferations: morphological, immunological and molecular analysis in seven patients.	the clinical, morphological, immunological and molecular features of seven patients with a stable picture of chronic granular lymphocytosis, observed over a period of up to 4 years, were studied. mild splenomegaly was detected in one patient, while lymphoadenopathy and hepatomegaly were absent. surface marker analysis showed in five patients the common membrane phenotype of granular t-cell lymphocytosis (t3+, t4-, t8+, leu-7+); of the remaining two, one presented an unusual phenotype (t3+, t4+, ...	1987	3496916
biochemical studies on a cell surface determinant involved in t-cell proliferation.	previous studies have shown that a monoclonal antibody (th5.2) recognizes a cell-surface determinant which is involved in the proliferative capability of t cells. the work reported here demonstrates that the t-cell-surface antigen recognized by th5.2 is a glycoprotein of 55,000 to 60,000 molecular weight. the molecule shows a single molecular weight species upon reduction and denaturation, and it contains only a few percent of tunicamycin-sensitive carbohydrate structures. as shown in sequential ...	1987	3496975
[factors of humoral and cellular immunity in onchocercosis (results of studies conducted in senegal)].	in the course of onchocerca volvulus infection in subjects with from light to heavy microfilarial count in skin snips occurred important alterations concerning both humoral and cell-mediated immunity. serum examination for immunoglobulins showed high synthesis of igg, igm and ige. the level of igm was found to be correlated to the intensity of the infection, an important reduction of t4 cells was observed and is associated with hyporeactivity to in vitro stimulation to phytohemagglutinin a (pha) ...	1987	3496980
lymphocyte surface phenotypes in pernicious anemia.	because an immunological defect of an unknown nature is thought to be a factor in the pathogenesis of pernicious anemia, we studied lymphocyte surface phenotypes in 40 patients and compared them with 113 healthy controls. the only significant difference that emerged was in their slightly decreased number and proportion of surface immunoglobulin (lambda chain) -bearing cells. of specific interest, the numbers of okt4+ and okt8+ lymphocytes and the t4+/t8+ ratios were not significantly different f ...	1987	3497016
effect of thyroxine in vivo on malic enzyme activity in liver cytosol of propylthiouracil and alpha-amanitin treated rats.	the activity of rat liver malic enzyme (l-malate: nadp oxidoreductase/decarboxylating, mdh, e.c. 1.1.1.40) after t4 or t4 + alpha-amanitin treatment was investigated in relation to nuclear specific binding of t4. specific binding of 125i-t4 to hepatic nuclear sites in rats at 21 days after thyreoparathyreoidectomy (tx) was significantly increased (p less than 0.01) and was restored within 24 hours after a single dose of t4. equilibrium association constants ka estimated in all experimental group ...	1987	3497023
prostaglandin e acts at two levels to enhance colony formation in vitro by erythroid (bfu-e) progenitor cells.	the prostaglandin e (pge) enhancement of erythroid colony formation by human bone marrow erythroid progenitor cells (bfu-e) is mediated by a t8+ subset of lymphocytes. medium was conditioned by bone marrow and blood t-lymphocytes and t-lymphocyte subsets (t8+, t8-, t4+, and t4- cells) in the absence or presence of pge1 in order to determine if the cells could release a cell-free source of erythroid colony enhancing activity and what the conditions for this release would be. the t-lymphocyte cond ...	1987	3497050
defective in vitro growth of the hemopoietic progenitor cells in the acquired immunodeficiency syndrome.	in addition to immunologic derangement, hematological abnormalities have been reported in the majority of patients with acquired immunodeficiency syndrome (aids). in this study 15 patients with aids or aids-related complex (arc) were evaluated for the in vitro growth of hemopoietic progenitor cells. in all patients a significant reduction of growth (mean +/- sem) of colony-forming unit-granulocyte, erythrocyte, macrophage, (megakaryocyte) (cfu-gem) (1.2 +/- 0.3), burst-forming unit-erythroid (bf ...	1987	3497175
recombinant interleukin 4 promotes the growth of human t cells.	recently, we reported the isolation of a cdna clone that encodes a polypeptide which has b cell and t cell growth factor activities. the amino acid sequence of this polypeptide deduced from the nucleotide sequence of the cdna clone showed significant homology with mouse b cell stimulating factor-1. because of its multiple biologic activities, it was designated interleukin 4 (il-4). here we describe the effects of supernatants of cos-7 mouse cells transfected with the il-4 coding cdna clone in a ...	1987	3497196
t cell subsets in the blast cell population in phytohemagglutinin-stimulated peripheral blood in vitro and in rheumatoid arthritis synovial fluid in vivo.	the immunomodulatory t4/t8 ratio was studied in the total and activated lymphocyte populations by a method combining visualisation of 3h-thymidine incorporating blasts with autoradiography (ar) with simultaneous identification of the respective lymphocyte subsets using monoclonal antibodies in avidin-biotin-peroxidase complex (abc) staining. in rheumatoid arthritis synovial fluid the activated t4/t8 ratio (calculated from t cells in the s phase of the cell cycle) was significantly different from ...	1987	3497421
effect of recombinant interleukin-2 on peripheral blood mononuclear cells following antigen-specific bronchial provocation.	in a group of 18 patients with seasonal asthma and rhinitis acute bronchial provocation was performed with grass pollen antigens. blood samples were taken before and 15 minutes after the maximal clinical response. effect of recombinant interleukin-2 (il-2) on peripheral blood mononuclear cells (pbmcs) by tritiated thymidine uptake method was investigated. t4 and t8 subpopulations were also evaluated before and after acute challenge. the patients had a significantly higher responsiveness of their ...	1987	3497565
t-cell subsets and natural killer cell function with squamous cell carcinoma of the head and neck.	twenty-two patients with squamous cell carcinoma of the head and neck were studied to assess potential abnormalities in total t lymphocytes, t4/t8 ratios, and natural killer cell function. total t-cell counts were reduced for the entire group with the greatest reduction occurring in individuals with hypopharyngeal tumors and/or stage iv disease. helper to suppressor ratios were within the norm for the total group, but specific site variances were detected in patients with oral cavity and hypopha ...	1987	3497652
[characteristics of the molecular organization of bacteriophage t4 whiskers].		1987	3497790
lymphocyte migration in the rheumatoid synovial membrane.	there are impressive similarities between the lymphocyte populations in lymph node and synovial membrane: similar amounts and classes of immunoglobulins are synthesized; lymphoid aggregates, when present in the rheumatoid synovial membrane, resemble those of the lymph node paracortex in being composed predominantly of helper-inducer t4 cells; b cells which leave tall endothelial pcv together with the t cells, tend to segregate themselves from the t cell aggregates and migrate to areas rich in ma ...	1987	3497900
expansion of activated t-lymphocytes in patients treated with recombinant interleukin 2.	recombinant interleukin 2 (ril 2, cetus) was administered in escalating doses to 30 patients with advanced malignancy, including 14 patients with the epidemic form of kaposi's sarcoma, in 2 week treatment cycles as a 6 h i.v. infusion for 10 doses. the maximum tolerated dose was 2 x 10(6) u/m2, with dose-limiting toxicity consisting of fever, diarrhea, and thrombocytopenia. at a well-tolerated dose of 1 x 10(6) u/m2, serum levels of ril 2 of 30 u/ml were maintained for the duration of the infusi ...	1987	3498011
motility and infiltration capacity of lymphoid tumour cells: disturbance of motile behaviour in tcll-lymphocytes.	the capacity of t-cells from 6 normal individuals and 4 cases of tcll to develop motile forms and migrate within a collagen matrix was compared. in order to exclude possible inhibitory or cooperative cellular interactions the lymphocytes were plated at low density. only a small proportion of fresh t-lymphocytes from healthy individuals developed motile forms after plating on substrata of collagen or plastic. during a 2 day culture period on collagen 50 to 90% of the t enriched cells from separat ...	1987	3498044
translational repression: biological activity of plasmid-encoded bacteriophage t4 rega protein.	the rega protein of bacteriophage t4 is a translational repressor that regulates expression of several phage early mrnas. we have cloned wild-type and mutant alleles of the t4 rega gene under control of the heat-inducible, plasmid-borne leftward promoter (pl) of phage lambda. expression of the cloned rega+ gene resulted in the synthesis of a protein that closely resembled phage-encoded rega protein in biological properties. it repressed its own synthesis (autogenous translational control) as wel ...	1987	3498046
effect of thyrotropin storage on thyroid-stimulating hormone response testing in normal dogs.	the stability of reconstituted, refrigerated thyrotropin was evaluated. thyrotropin (tsh) was reconstituted at the start of the study and stored at 4 degrees c. a tsh stimulation test was performed in eight healthy, euthyroid dogs at weekly intervals for 1 month. in seven of eight dogs, there was no significant difference (p less than 0.05) between the post-tsh t3 concentrations and the post-tsh t4 concentrations for the duration of the study. for one dog, the post-tsh t4 concentration was below ...	1987	3506093
serum thyroid hormone concentrations fluctuate in cats with hyperthyroidism.	we measured serum thyroxine (t4) and 3,3',5-triiodothyronine (t3) concentrations in hyperthyroid cats (hourly for 10 hours in 14 cats, and daily for 15 days in seven cats) to assess fluctuation in thyroid hormone levels. over the 10-hour study period the coefficient of variation (cv) for serum t4 and t3 concentrations ranged from 6.4-22.6% (mean = 12.0 +/- 4.8%) and from 9.6-33.1% (mean = 17.5 +/- 6.3%), respectively. during the 15-day study period, cv for serum t4 ranged from 6.6-34.8% (mean = ...	1987	3506098
the role of cysteine oxidation in the thermal inactivation of t4 lysozyme.	wild-type t4 lysozyme contains unpaired cysteine residues at positions 54 and 97. to investigate the role these residues play in the thermal inactivation of the wild-type, we constructed a double mutant with these cysteines replaced with valine and serine. this molecule, t4 lysozyme (c54v/c97s), is more stable than the wild-type to inactivation at 70 degrees c at ph 6.5 and 8.0. guanidine hydrochloride reactivation experiments and sds-page on the inactivated products show that the wild-type is s ...	1987	3507692
use of site-directed mutagenesis to obtain isomorphous heavy-atom derivatives for protein crystallography: cysteine-containing mutants of phage t4 lysozyme.	five different cysteine-containing mutants of the lysozyme from bacteriophage t4 were used to explore the feasibility of using site-directed mutagenesis to generate isomorphous heavy-atom derivatives for protein crystallography. cysteines 54 and 97, present in wild-type lysozyme, can be readily reacted with mercuric ion to produce an excellent isomorphous heavy-atom derivative. mutants with an additional cysteine at position 86, 146, 153 or 157, or with cys 97 replaced by val, were engineered by ...	1987	3507694
amperometric enzyme-immunosensor based on ferrocene-mediated amplification.	coimmobilized glucose oxidase and anti-t4 antiserum are employed in the construction of a biosensor for thyroxine measurements. the resulting enzyme-immunosensor yields an amplified response to t4 with a detection limit of 15 nm.	1987	3507858
postnatal changes in circulating concentrations of growth hormone, somatomedin c and thyroid hormones in pigs.	plasma concentrations of growth hormone (gh), somatomedin (smc), thyroxine (t4) and triiodothyronine (t3) were determined from birth to post weaning in pigs (yorkshires). plasma samples were obtained from the smallest, median and largest piglet from 11 litters. no differences in the circulating concentrations of any of the hormones were observed between piglets of different sizes. however, there were changes in circulating concentrations of hormones during postnatal development. plasma concentra ...	1987	3507893
thyroid hormone alterations in patients with shock and injury.	serum concentrations of total t3 and t4, free t4, rt3, tsh, tbg and cortisol were measured on arrival in hospital in 33 adult injured patients, 26 of whom were received directly from the accident. serum cortisol levels and all thyroid indices, except tbg, were altered substantially by injury. compared with values from 57 healthy volunteers, statistically significant (p less than 0.01) decreases were found in the mean serum free and total t4 concentrations and the rt3 level. similarly significant ...	1987	3508880
modulation of gamma-aminobutyric acid uptake of rat brain synaptosomes by thyroid hormones.	the effects of thyroid hormones on gamma-aminobutyric acid (gaba) uptake were compared in homogenates and purified synaptosomes from rat cerebral cortex. l-triiodothyronine (t3) and l-thyroxine (t4), but not reverse t3(rt3), blocked gaba uptake in homogenates when hormone and labeled gaba were added to the system simultaneously. similar effects were obtained when homogenates were preincubated with hormone and washed to remove free hormone before addition of gaba. a competitive inhibitor of neuro ...	1987	3509068
[propofol in the surgery of the aortic bifurcation. comparison with isoflurane].	two groups of nine patients each, all undergoing surgery for aorto-bifemoral prosthesis, were studied. they were premedicated with 10 mg morphine and 1 mg flunitrazepam. a swan-ganz catheter was then inserted. cardiac output was measured by thermodilution. haemodynamic parameters were measured before induction (t0), 15 min after intubation (t1), 10 min after the start of the surgical procedure (t2), 5 min before clamping the aorta (t3), whilst the aorta was clamped (t4), and a few minutes after ...	1987	3498394
in vivo labeling of blood t cells: rapid traffic into cerebrospinal fluid in multiple sclerosis.	multiple sclerosis (ms) is an inflammatory disease of the central nervous system (cns). in ms patients being treated with anti-t11, a murine monoclonal antibody which recognizes the sheep red blood cell receptor, it was found that peripheral blood t cells were labeled in vivo by the antibody. furthermore, anti-t11 did not lyse cells or enter the cerebrospinal fluid (csf). in csf specimens obtained by serial lumbar punctures from patients with progressive ms who received five daily infusions of a ...	1987	3498435
iron-related disturbances of cell-mediated immunity in multitransfused children with thalassemia major.	immunological abnormalities have been observed in many haemophiliacs receiving clotting factor concentrates. to determine whether similar changes also occur after repeated blood transfusions we estimated t cell subsets and cutaneous delayed hypersensitivity (cdh) in 50 multitransfused children with beta-thalassemia major (beta-tm). all patients were also tested for anti-htlv-iii/lav antibodies. a diminished percentage of t lymphocytes (e-rosettes, t3+), and t4+ cells and a low t4/t8 ratio was fo ...	1987	3498564
abnormalities in the immune system of children with beta-thalassaemia major.	we have studied both the humoral and cell mediated immune systems of 23 children with beta-thalassaemia major. in children who had not been splenectomized, a 3-fold expansion in the number of circulating b cells and a modest polyclonal gammopathy was present. of these patients 70% had decreased numbers of circulating t4 cells; 83% were unresponsive to skin testing with candida albicans, and the majority had decreased lymphocyte proliferative responses in vitro. in children who had been splenecto ...	1987	3498580
lymphocyte subpopulations in donovanosis assessed by monoclonal antibodies and immunoglobulins.	the haematological profile and peripheral blood lymphocyte subpopulations of 36 patients with donovanosis were estimated by an indirect immunofluorescence technique using monoclonal antibodies. concentrations of serum immunoglobulins (igm, igg, and iga) were also estimated. the total leucocyte and lymphocyte counts were generally raised, as were total t lymphocyte, t4, and t8 counts. patients with the hypertrophic variant, however, had decreased numbers of t lymphocytes and t8 cells and increase ...	1987	3498678
expansion of a t lymphocyte subpopulation (cd3+,4-,8-) after immunodepression associated with disseminated histoplasmosis.	a major subpopulation of t lymphocytes bearing a high density of cd3 (t3/leu 4) with no detectable cd4 (t4/leu 3a) or cd8 (t8/leu 2a) was found in a patient with cardiomyopathy. previously the patient had developed disseminated histoplasmosis which had been associated with a profound anergic state. the proportion of cd3+ lymphocytes that bore the phenotype cd3+,4-,8- has been increasing in the absence of reactivation of the histoplasmosis. the cd3+,4-,8- lymphocytes bound anti-cd2 but did not bi ...	1987	3498697
unresponsiveness to skin testing with bacterial antigens in patients with haemophilia a not apparently infected with human immunodeficiency virus (hiv).	unresponsiveness to skin testing with ppd and tetanus toxoid was commonly seen in patients with haemophilia a but not infected with human immunodeficiency virus but was uncommon in controls. vaccination history indicated that the unresponsive patients had not been immunised in childhood. other tests of immune competence (skin tests with other antigens, lymphocyte stimulation with mitogens and antigens, and viral serology) showed that the haemophilia a patients had an adequate response to pathoge ...	1987	3498742
interleukins in chronic active hepatitis b. relationship with viral markers.	interleukin-2, a product of helper t cells, is essentially involved in the regulation of cell-mediated immunity. two monocyte-derived factors, interleukin-1 and prostaglandin e2, influence interleukin-2 synthesis with opposite actions. to analyse immunoregulatory function in hbsag-positive chronic active hepatitis, t cell subsets in peripheral blood and the levels of interleukin-2, interleukin-1 and prostaglandin e2 in supernatants from lectin- or lipopolysaccharide-activated peripheral mononucl ...	1987	3498750
hyperthyroxinemic mice have reduced natural killer cell activity. evidence for a defective trigger mechanism.	natural killer (nk) activity of peripheral blood lymphocytes from hyperthyroxinemic patients (graves' disease or thyroxine (t4)-treated) is severely depressed. in order to study the relationship of thyroid hormone to nk activity, a model for hyperthyroxinemia was induced in mice by addition of t4 to the drinking water. control mice were hypothyroid (fed propylthiouracil) or normal. serum t4 levels were elevated (within 2 wk) in mice fed thyroid hormone. six weeks after initiation of the diets, i ...	1987	3498760
effect of thyrotrophin on epidermal growth factor receptors in monolayer cultures of porcine thyroid cells.	primary cultures of porcine thyroid cells, grown as monolayers, showed saturable binding of epidermal growth factor (egf). scatchard analysis resolved the binding to a high-affinity/low-capacity site (dissociation constant = 0.17 nmol/l, maximal binding capacity = 1.67 pmol/10(6) cells) and a low-affinity/high-capacity site. preincubation of thyroid monolayers with tsh for 3 days caused an increase in binding of 125i-labelled egf due to an increase in the number of receptors, with the binding af ...	1987	3498782
isolation and reassembly of bacteriophage t4 core proteins.	the products of genes 22, 67 and 68, and the internal proteins ipi, ipii and ipiii, as components of the scaffolding core of the bacteriophage t4 prohead, have been isolated and purified by hydroxylapatite column chromatography. under conditions promoting reassembly in vitro, the proteins associated into elongated particles of practically constant width but variable length that we have called polycores. preliminary optical diffraction experiments indicate that polycores may have an ordered struc ...	1987	3498844
gene amplification mechanism for the hyperproduction of t4 bacteriophage gene 17 and 18 proteins.	bacteriophage t4 mutants hyperproducing gene 17 protein (hp17) have been isolated at high frequency by growing gene 17 amber mutants on ochre suppressor strains of escherichia coli. most mutants showed the co-hyperproduction of gene 18 protein, although one anomalous mutant hyperproduced a 60,000 mr partial polypeptide of gene 18. hybridization of t4 late rnas to cloned plasmid dna containing genes 17, 18 or control t4 genes revealed that approximately five times more gene 17 mrna and two to thr ...	1987	3498845
pyridoxine supplementation: effect on lymphocyte responses in elderly persons.	the effect of pyridoxine supplementation on lymphocyte responsiveness was investigated in 15 persons aged 65-81 y. eleven subjects received 50 mg/d pyridoxine hcl (pn). four subjects received a placebo. lymphocyte proliferation to t and b cell mitogens, lymphocyte subpopulations with monoclonal antibodies, and plasma pyridoxal 5'-phosphate (plp) were measured before and after 1 and 2 mo of supplementation. after 1 and 2 mo plasma plp levels increased by 195 +/- 88 nm and 201 +/- 84 nm, respectiv ...	1987	3499066
natural killer cell activity and lymphocyte function during and after coronary artery bypass grafting in relation to the endocrine stress response.	the effects of elective coronary artery bypass grafting (cabg) and the associated endocrine stress response on natural killer (nk) cell activity in peripheral blood, the distribution of lymphocyte subpopulations, and the phytohemagglutinin (pha)-induced lymphocyte transformation were studied in 20 patients anesthetized with either etomidate-high dose fentanyl (75-125 micrograms . kg-1) or midazolam-low dose fentanyl (less than 20 micrograms . kg-1). the endocrine response to surgery was measured ...	1987	3499099
2,000-centigray total lymphoid irradiation for refractory rheumatoid arthritis.	because toxicity with the use of 3,000 centigray (cgy) of total lymphoid irradiation (tli) was observed in an earlier study, 2,000-cgy treatments were delivered in a 2-portal format to 7 patients and in a modified 3-portal fashion to 6 patients, as part of a randomized, investigator-blinded trial of tli treatment for refractory rheumatoid arthritis. analysis of combined data from the 13 patients revealed statistically significant improvement in 5 clinical indicators of disease activity at the en ...	1987	3499154
alterations in t-lymphocyte subpopulations in patients with head and neck cancer. correlations with prognosis.	impaired cell-mediated immunity has been consistently demonstrated in patients with advanced head and neck squamous cancer (hnsc); however, the results of prior studies of correlations of cellular immune parameters with treatment outcome have been inconsistent, and routine assessment of immune parameters has been of limited clinical use. to determine the prognostic importance of alterations in the proportions of various t-lymphocyte subpopulations in the peripheral blood of patients with hnsc, l ...	1987	3499156
complement and anaphylatoxin responses to cross-clamping of the aorta. studies during general anaesthesia with or without extradural blockade.	complement activation and anaphylatoxin formation were studied in 28 patients undergoing aortic reconstructive surgery under general anaesthesia. in 12 of the patients an extradural catheter was placed in the l1-2 space and 2% mepivacaine was injected until blockade reached the t4 level. the remaining 16 patients were operated upon under general anaesthesia alone. complement activation was not obvious until aortic cross-clamping. the release of anaphylatoxin was less pronounced during cross-clam ...	1987	3499158
chromosome abnormalities in peripheral t-cell lymphoma.	we have studied neoplastic lymph nodes from six patients histologically, immunologically and cytogenetically. histologically all the cases were classified as peripheral t-cell lymphomas. these were subclassified as t-zone lymphomas in three, large cell 'pale cell' variant in one, large cell immunoblastic in one, and small cell, mycosis fungoides in one. two had features of angioimmunoblastic lymphadenopathy (aild). immunologically all cases expressed cd2 (okt11) and cd4 (t4). all six cases had c ...	1987	3499166
diagnostic and prognostic value of monoclonal antibodies in immunophenotyping of angioimmunoblastic lymphadenopathy/lymphogranulomatosis x.	immunophenotyping of frozen lymph node sections from seven patients with morphologically defined angioimmunoblastic lymphadenopathy (aild) was performed with a panel of 20 monoclonal antibodies (moab). moab for identification of dendritic reticulum cells showed remnants of follicular structures in all cases. in four cases these 'burnt out' follicular structures were associated with clusters of polyclonal b-cells. vascular proliferation and small amounts of intercellular collagenous fibrils were ...	1987	3499172
a possible cytotoxicity inducer role of 5/9 positive lymphocytes infiltrating the liver in cah patients.	the presence, in chronic active hepatitis (cah) patients, of an inflammatory infiltrate basically composed of t lymphocytes suggested the hypothesis that these lymphocytes could play a role in the pathogenesis of the disease. the aim of this study has been to characterize, in a group of carefully selected cah patients, the liver-infiltrating t lymphocyte, utilizing commercial monoclonal antibody (anti-leu 1, anti-leu 2a, anti-leu 3a) and 5/9 monoclonal antibody that recognizes a further lymphocy ...	1987	3499278
growth impairment caused by dietary nitrate intake regulated via hypothyroidism and decreased somatomedin.	the effect of 2 days or 6 weeks administration of the diet containing 3 per cent potassium nitrate on serum level of thyroxine (t4), triiodothyronine (t3), nitrate, methemoglobin and somatomedin activity was investigated in piglets aged 56 days. sufficient iodine intake by mothers prevented a decrease of t4 level in piglets after 2 days administration of nitrate. however, after 6 weeks of nitrate administration a striking decrease of t4 level was found (average level less than 10 nmol l-1) which ...	1987	3499306
immunoglobulin levels in haemophiliacs at hiv seroconversion and during follow up.	immunoglobulins and aminotransferases were followed in 66 haemophilia patients and 13 von willebrand patients over a six-year period. the results were correlated to hiv serology and lymphocyte subsets. elevated igg levels were found in 29/53 patients with haemophilia a, 2/13 with haemophilia b and in 0/13 with von willebrand's disease. elevated iga and igm levels were seen in 20% and 27% of the patients respectively, with a distribution similar to the elevated igg levels, except that elevated ig ...	1987	3499399
effect of altered thyroid hormone levels on hypothalamic-pituitary-adrenal function.	to determine whether alterations in serum thyroid hormone levels affect hypothalamic-pituitary-adrenal function, we measured the plasma immunoreactive (ir) acth and ir-cortisol responses to 1 microgram/kg bw ovine crh (ocrh) given iv in the late afternoon and the plasma ir-acth, ir-cortisol, and ir-11-deoxycortisol responses to 2 g metyrapone given orally at midnight in 10 athyreotic patients during t4 treatment and 1 month after stopping t4 when they were biochemically, but not clinically, hypo ...	1987	3499449
effect of alpha-interferon on the immune system of patients with hairy cell leukemia.	the role of in vivo administration of lymphoblastoid alpha-interferon (ifn-alpha) on the immune status of 12 patients with hairy cell leukemia (hcl) was studied. in most cases an increase in t3(cd3), t4(cd4), and t8(cd8) lymphocyte subsets was documented at the same time as hairy cells disappeared from the circulation. this led in most cases to an improved t4/t8 ratio. the effect of treatment with ifn-alpha was particularly evident on the natural killer cell compartment, which is often functiona ...	1987	3499543
phenotypic characterization of mononuclear cell infiltrate in chronic active hepatitis (cah) of different etiology.	the morphologic characterization of portal and lobular infiltrates of chronic active hepatitis (cah) was performed on liver biopsy sections from 18 patients: 6 hbsag-positive/anti-delta-negative with tissue signs of hepatitis b virus (hbv) replication, 6 hbsag-positive/anti-delta-positive with tissue signs of hepatitis delta virus (hdv) replication, and 6 hbsag-negative without clinical or serologic evidence of autoimmune disease. no statistically significant differences were found among all the ...	1987	3499652
cellular kinetics of delayed hypersensitivity test reactions to topical glucocorticosteroids.	the phenotypes of the infiltrating cells in 13 patients with delayed hypersensitivity to topical glucocorticosteroids (gcs) were studied from sequential biopsies of positive epicutaneous test reactions by using the avidin-biotin-complex (abc) technique. monoclonal antibodies were used to identify the cells with the following phenotypes: t3, t4/t4a, t6, t8, t9, t11, m1, ia1 (hla-dr), interleukin-2 receptor/t26a, and dendritic reticular cell. the cellular kinetics of gcs hypersensitivity reactions ...	1987	3499871
lymphocyte subpopulations in bronchoalveolar lavage in sjögren's syndrome. evidence for an expansion of cytotoxic/suppressor subset in patients with alveolar neutrophilia.	we initiated this study to determine the cellular composition and t-lymphocyte subpopulations of fluid from bronchoalveolar lavage from 15 patients with primary sjögren's syndrome (1ss), six patients with secondary sjögren's syndrome associated with primary biliary cirrhosis (2ss-pbc), eight patients with secondary sjögren's syndrome associated with collagen-vascular diseases (2ss-cvd), and 12 normal subjects. all were nonsmokers who were free of clinical pulmonary symptoms and had normal findin ...	1987	3500017
expression of natural killer cell markers in non-hodgkin's lymphomas.	one hundred forty-nine cases of non-hodgkin's lymphoma were studied with a panel of monoclonal antibodies, including antibodies to natural killer (nk) cells--anti-nkh1, anti-leu 7, and anti-leu 11b. there were 95 b-cell, 51 t-cell, and three null cell lymphomas. seventeen t-cell lymphomas (33 per cent) expressed nkh1, leu 7, and/or leu 11b. none of the b- or null cell lymphomas expressed the nk markers. in comparison with the nk-negative t-cell lymphomas, the nk-positive cases showed a predilect ...	1987	3500108
t lymphocyte control of human eosinophilic granulopoiesis. clonal analysis in an idiopathic hypereosinophilic syndrome.	the idiopathic hypereosinophilic syndrome (hes) comprises a heterogeneous group of disorders with unknown pathogenesis characterized by persistent peripheral blood and bone marrow eosinophilia and eosinophilic infiltrates of multiple organs leading to severe organ dysfunction. in the present study, t lymphocyte clones were randomly established from the blood of a patient with hes and propagated in culture with mitogen and interleukin 2. whereas 28 of 29 clones were able to stimulate myeloid colo ...	1987	3500229
activation and long-term proliferation of human cord blood t cells with human recombinant interleukin-2.	we report here the results of in vitro studies that demonstrate that purified human recombinant interleukin-2 (hril-2) is a potent stimulant for freshly isolated cord blood lymphocytes of healthy full-term infants. different culture parameters were studied to define the optimal conditions for eliciting maximal levels of activation. highest levels of hril-2-induced tdr[3h] uptake were recorded on day 7 for cultures containing 100 u hril-2/ml. results of comparative studies demonstrated that the r ...	1987	3500315
downregulation of cell surface molecules during noncytopathic infection of t cells with human immunodeficiency virus.	noncytopathic infection of human t-lymphoid cell line cr-10 with human immunodeficiency virus (hiv) (cem-n1t isolate) resulted in a gradual loss of cell surface receptors for okt4/okt4a (hiv receptor), okt8, okt3, and okt11 but not for okt9 (transferrin receptor) within 10 days after infection. surface receptor decline was accompanied by a rapid increase in hiv antigens and mrna expression. multireceptor downregulation was also observed in three t-lymphoid cell lines (mt-4, cem, and hbd-1) cytop ...	1987	3500327
aids and lymphadenopathy syndrome (las) patients display similar abnormal in vitro proliferation and differentiation of t-colony forming cells (t-cfc).	t-cell colonies were generated from the peripheral blood and bone marrow of 61 patients with acquired immunodeficiency syndrome (aids), 54 patients with persistent lymphadenopathy syndrome (las), 14 clinically normal male homosexuals, and 17 healthy heterosexuals. mononuclear cells were cultured in methylcellulose in the presence of il2-containing conditioned medium. the number of t-cell forming cells (t-cfc) from healthy male homosexuals and aids and las patients was significantly (p less than ...	1987	3500783
human epidermal growth factor concentrations in urine, but not in saliva and serum, depend on thyroid state.	to evaluate the effects of thyroid hormones on the concentration of epidermal growth factor (egf), we determined values for the immunoreactive egf concentration in the urine (u-iregf) of newborn infants with congenital hypothyroidism (n = 19), and in urine, saliva and serum of adult patients with hypothyroidism (n = 11) and hyperthyroidism (n = 8). the values were expressed as sd score (sds), i.e. deviation in sd units from their mean value of healthy subjects of the same age and sex. the sds of ...	1987	3501054
structure and expression of the human and mouse t4 genes.	the t4 molecule may serve as a t-cell receptor recognizing molecules on the surface of specific target cells and also serves as the receptor for the human immunodeficiency virus. to define the mechanisms of interaction of t4 with the surface of antigen-presenting cells as well as with human immunodeficiency virus, we have further analyzed the sequence, structure, and expression of the human and mouse t4 genes. t4 consists of an extracellular segment comprised of a leader sequence followed by fou ...	1987	3501122
local immune responses after intravesical bcg treatment for carcinoma in situ.	the lymphoid cellular infiltrates in the bladder biopsies from patients with carcinoma in situ before and after bcg intravesical therapy have been determined and characterised. this has been achieved using a panel of monoclonal antibody probes in an indirect immunoperoxidase technique. these studies have revealed the predominance of t-cells of the helper/inducer phenotype (t4+), beta-lymphocytes (b1+) and macrophages (leu m3+, 3.9+) in bladder biopsies after bcg therapy. hla.dr antigens were als ...	1987	3501324
exchange transfusion in premature newborns: effect of maturity on thyrotropin and thyroxine responses.	the effects of exchange transfusion on plasma t4 and tsh were studied in two groups of premature newborns to evaluate the effect of maturation on the reactivity of the pituitary-thyroid axis. in newborns with a birth weight between 1900-2500 g (gestational age 35.4 +/- 0.45 weeks) the responses of both hormones were essentially similar to those reported previously for mature newborns (a profound decrease during the procedure and an increase 24 h later). in a group of newborns with a birth weight ...	1987	3501980
thyroid disorders in the general population of hisayama japan, with special reference to prevalence and sex differences.	serum triiodothyronine (t3), thyroxine (t4), thyroid stimulating hormone (tsh) and thyroid autoantibodies (taa) to either microsome or thyroglobulin were measured in 1251 samples from the general population, 546 males and 705 females, aged 40 or over in hisayama, a japanese rural town. taa was positive in 7.7% of men and 15.0% of women, and the male/female ratio was 1:2. this ratio was markedly different from those in hospital patients with thyroid disorders where ratios such as 1:8 for chronic ...	1987	3501990
[immunological and trace element study in 50 children with various diseases caused by food allergens and aeroallergens].	the authors executed a study on the levels of t lymphocytes and of serum fe, zn and cu in 50 atopic children. the boys showed a various pathology who included the eczema, the laryngotracheobronchostenosis, the choanal obstruction and/or the adenoid's obstruction, abdominal pains, stomatitis, relapsing herpes, shock, neurosis. the t4/t8 relationship resulted lower in the 34.5% of subjects, higher in the 30.5% normal in the 35.5%. in the patients with hyper-ige the 28.2% of children showed this re ...	1987	3502023
[neurohormonal and immunological status of patients with autoimmune thrombocytopenic purpura].	a study was made of the relationship between hormonal (acth, cortisol, tsh, t3, t4, sth) and immune (e-rfc, eac-rfc, and immunoglobulins) shifts in the time course of therapy in 106 patients with autoimmune thrombocytic purpura. a significant decrease in the level of t3 and its direct correlation with e-rfc in exacerbation of disease combined with an increase in the levels of cortisol and igm were revealed. the treatment with glucocorticoids and immunosuppressants resulted in a more noticeable d ...	1987	3502295
t4 lymphopenia in human tuberculosis.	lymphocyte subpopulations in vitro in 13 patients with bacteriologically-proven tuberculosis and 12 matched controls, by immunofluorescence using monoclonal antibodies have been studied. active tuberculosis was associated with significant reductions in absolute numbers of total t (leu 4 or 1+), t4 (leu 3a+) and b (leu 12+) lymphocytes, but there were no significant differences in total t8 (leu 2a+) counts. in two patients, t4-lymphopenia was sufficiently profound to cause reversal of t4: t8 rati ...	1987	3502379
enhancement of cytotoxic and proliferative responses of lymphocytes from melanoma patients by incubation with monoclonal antibodies against ganglioside gd3.	previous studies have shown that monoclonal antibodies (m.ab) to the ganglioside gd3 may induce partial remissions in tumour growth in patients with melanoma. in vitro studies demonstrated that m.abs to gd3 may also enhance lymphocyte responses to phytohemagglutinin and interleukin 2 (il2). the present study extended these findings by showing that the il2-dependent proliferative and cytotoxic response of t cell clones derived from a melanoma patient and a patient with the vogt-koyanagi-harada sy ...	1987	3493846
characterization of virus-specific cytotoxic t cell clones from allogeneic bone marrow chimeras.	we established several h-2-restricted lymphocytic choriomeningitis virus (lcmv)-specific cytotoxic t cell clones from spleens of virus-primed c57bl/6 or c57bl/10 (h-2b) and b10.br (h-2k) mice and from allogeneic c57bl/10----b10.br and b10.br----c57bl/10 bone marrow chimeras. two t cell clones of h-2b origin and restricted to h-2b, 3 of h-2k origin and restricted to h-2k were compared with two clones each derived from the two types of chimeras. their surface phenotype was found to be lyt-2+, l3/t ...	1987	3493906
expression of the t4 molecule (aids virus receptor) by human brain-derived cells.	three human cell lines of astrocytic origin were evaluated for expression of a human t-lymphocyte surface glycoprotein, t4, which also serves as a cellular receptor for the human immunodeficiency virus (aids virus, hiv). t4 antigen was detected on the cell surface of 2 of these cell lines using monoclonal okt-4 antibody and flow cytometry. gene transcripts encoding the t4 molecule were detected by a ribonuclease protection assay in surface t4-positive and -negative cells. our results suggest tha ...	1987	3493919
t-lymphocyte subsets in primary lung cancer.	t-cell subsets in the peripheral blood of 63 primary lung cancer patients (23 with adenocarcinoma, 23 with squamous cell carcinoma and 17 with small cell carcinoma) and 24 normal healthy controls were determined by indirect immunofluorescence, using the monoclonal antibody reagents okt3, okt4 and okt8. correlations between t-lymphocyte subset values and stages or cell types of disease were sought. total lymphocytes in the patient group were decreased. however, no significant difference from cont ...	1987	3494139
ph1-positive cml-derived myeloid-monocytoid precursor cell line producing substance(s) that stimulates normal cfu-c.	a new ph1-chromosome positive cell line, kopm-28. was established from a patient with chronic myelogenous leukemia (cml) in blast crisis. kopm-28 cells were phenotypically immature: without azurophilic granules; negative for myeloperoxidase and positive for specific and nonspecific esterases. the nonspecific esterase reaction was intensified by tpa, and retinoic acid reinforced the specific esterase reaction without inducing morphological changes. kopm-28 cells were not phagocytic. the cells exp ...	1987	3494166
production of human pluripotent progenitor cell colony stimulating activity (cfu-gemmcsa) in patients with myelodysplastic syndromes.	the present study was aimed at assessing the possible relationship between the t-lymphocyte abnormalities and the stem cell dysfunction in myelodysplastic syndromes (mds), by investigating the production of specific stimulators of stem cell differentiation in such patients. conditioned media from peripheral blood mononuclear cells (pbmnc) of mds patients and healthy controls, prepared with or without phytohaemagglutinin (pha), were assayed for their capacity to stimulate the in-vitro formation o ...	1987	3494167
peripheral t cell lymphoma.	thirty-one chinese patients with peripheral t cell lymphoma (ptcl) were reviewed. using the modified japanese lymphoma group classification, there were nine (29%) of the pleomorphic type, 16 (52%) immunoblastic lymphadenopathy (ibl)-like, two (7%) t-zone lymphoma, and one (3%) lennert's lymphoepithelioid type. three (9%) were not classifiable. all were positive for t11 (e rosette receptor antigen). fifty-four percent (15 of 28) were positive predominantly for t4 (helper t cell) and 46% (13/28) f ...	1987	3494819
immune rosettes of t and b lymphocytes in infertile women with endometriosis.	we examined the cell-mediated immune response in women with pelvic endometriosis by quantitating their t and b lymphocytes. the study was conducted on heparinized peripheral blood and peritoneal fluid. the results were compared with those in a control group of women without evidence of endometriosis. each patient underwent laparoscopy as part of her infertility evaluation. the ability of t cells to form rosettes with sheep erythrocytes was used to estimate the percentage of total and active t ce ...	1987	3494842
relationship between thyroid hormones and nutrition in chronic renal failure.	a cross-sectional study was performed in 19 patients on haemodialysis and in 11 on continuous ambulatory peritoneal dialysis (capd) in order to investigate the relationship between thyroid hormones and nutritional status. t4, t3 resin uptake, t3, rt3 and tsh were measured by radio-immunoassay and compared with controls. nutritional status was assessed by measurements of blood proteins, albumin, gamma-globulin, transferrin, arm muscle circumference and triceps skinfold thickness. in haemodialysis ...	1987	3494956
[distribution of t-lymphocyte subpopulations in non-hodgkin's lymphomas].	percentage okt3, okt11+, okt4+ and okt8+ lymphocytes were measured in the peripheral blood of 16 patients with untreated non-hodgkin b lymphoma aged 37-63 (11 males and 5 females). patients were classified according to the histological type and clinical stage of the disease. in all cases, t3+, t4+, t8+ and t11+ levels were higher then in the controls but the okt4/okt8 ratio remained normal in 14 out of 16 cases.	1987	3494961
lymphocyte major histocompatibility complex-encoded class ii structures may act as sperm receptors.	human sperm and blood cells were cocultured in vitro to determine whether specific interactions occur between gametes and blood cells. evidence for cell type-specific sperm binding and penetration of lymphocytes is presented together with findings that suggest that either or both events involve major histocompatibility complex-encoded class ii molecules on lymphocytes and a sperm ligand that is immunoreactive with antibodies to t-cell surface antigen t4. involvement of hla-dr is suggested by the ...	1987	3494998
the role of t4 in immune function and dysfunction.		1987	3495278
thyroxine accelerates the differentiation of granular convoluted tubule cells and the appearance of epidermal growth factor in the submandibular gland of the neonatal mouse. a fine-structural immunocytochemical study.	the effects of the administration of thyroxine (t4) on the postnatal cytodifferentiation of granular convoluted tubule (gct) cells of the submandibular gland (smg) of lewiss-webster mice were studied by light and electron microscopy. from birth, mice of both sexes were injected daily with t4 (sc 0.4 micrograms/g bw) and were sacrificed 24 h after the last injection at 7, 9, 11, 14 and 21 days of age. control mice received vehicle only. in control mice, granulated striated duct (sd) cells were fi ...	1987	3495340
human t cell subset ratio quantitation by 51cr release: assessment of a new technique.	a method for t cell subset ratio quantitation is described. this technique makes use of monoclonal antibodies 66.1 (okt4 equivalent) and 51.1 (okt8 equivalent) in complement-dependent cytotoxicity. the percentages of 51cr release with these 2 reagents from peripheral blood lymphocyte targets were used to calculate the ratio. it is simple, inexpensive, and can be used for large numbers of samples, but is less accurate when compared with the microscopic technique in immunofluorescence. it may serv ...	1987	3495381
abnormal cellular and humoral immunity in childhood acute lymphoblastic leukemia in long-term remission.	the effects of multiagent chemotherapy were evaluated in 36 patients with acute lymphoblastic leukemia in long-term remission. decreased numbers of neutrophils, lymphocytes, t cells and t-cell subpopulations (t(m), t(g), t4 [helper] and t8 [suppressor]) were found. additional abnormalities were reduced numbers of natural killer (nk) cells, assayed using the myeloid/erythroid k562 cell line, and impaired immunoglobulin production by pokeweek mitogen (pwm)-stimulated, cultured mononuclear cells. p ...	1987	3493591
peripheral t cell lymphoma presenting as hypereosinophilia with vasculitis. clinical, pathologic, and immunologic features.	peripheral t cell lymphoma developed in two patients several years after an initial clinical presentation of eosinophilia, pruritic skin rash, and vasculitis with lymphocytic infiltrates. despite treatment with combination chemotherapy, the patients survived less than six months after the malignant lymphoma emerged. immunologic characterization of tumor cells demonstrated features characteristic of peripheral t cell lymphoma. t lymphocytes from one patient had the uncommon phenotype of t3-negati ...	1987	3493692
the selective activation of t8+ cells by neuraminidase and galactose oxidase is mediated by activated t4+ cells.	the response of highly enriched populations of human t8+ lymphocytes to the oxidative mitogenic enzymes neuraminidase (na) and galactose oxidase (go) was enhanced by nago-primed t4+ lymphocytes. no similar enhancement occurred when the cells were primed with phytohemagglutinin (pha). in the absence of subclass contamination (1%), the t8+ and t4+ cells responded equally to nago by the criterion of dna replication. the addition of a small number, 2-10%, of nago-t4+ cells to the nago-t8+ cells enha ...	1987	3494623
the effects of experimental hypo- and hyperthyroidism on blood viscosity and other blood parameters in the rat.	three groups of male sprague dawley rats received methimazole without or with na-thyroxine in drinking water (3 and 0.33 mg t4/l, respectively) to induce characteristic alterations of their thyroid status (hypothyroid, hyperthyroid, euthyroid). a fourth group served as an untreated control without any additive to the drinking water. with respect to the different thyroid status, the following changes in the blood parameters were found: increasing plasma-t3-levels caused a reduction in plasma visc ...	1987	3502771
[quality control of the radioimmunoassay of t3, t4 and tsh].		1987	3504636
prohead core of bacteriophage t4 can act as an intermediate in the t4 head assembly pathway.	bacteriophage t4 assembly was impaired in escherichia coli hdb3-1 at an incubation temperature below 30 degrees c. naked prohead cores (head scaffold) bound to the inner surface of the plasma membrane accumulated, and the major shell protein (gp23) precipitated into visible intracellular aggregates in the cytoplasm. shifting the temperature to 42 degrees c allowed newly synthesized gp23 to assemble around the accumulated cores. we conclude that synchronous assembly of the scaffold and shell is n ...	1987	3537341
graft vs graft reaction resulting in the elimination of maternal cells in a scid patient with maternofetal gvhd after an hla identical bone marrow transplantation.	in a young girl with a severe combined immunodeficiency, the presence of circulating maternal t lymphocytes was proven by hla typing. manifestations of skin graft vs host disease were associated with the persistence of maternal cells. the patient received an hla identical bone marrow transplantation from her brother without any conditioning. the bone marrow transplantation was quickly followed by a transient and dramatic increase in skin lesions associated with fever and the finding of a high nu ...	1987	3540121
t-lymphocyte subsets in renal allograft recipients treated with cyclosporine and azathioprine.	thirty-nine renal allograft recipients were prospectively studied to determine the quantitative effects of different immunosuppression protocols on t-cell subsets (total lymphocytes [t3], helper/inducer [t4] and suppressor/cytotoxic [t8]). eighteen patients were initially immunosuppressed with only azathioprine and prednisone but required subsequent treatment for rejection by the addition of antithymocyte globulin (atg) (upjohn, kalamazoo, mi) or conversion to cyclosporine. three of these patien ...	1987	3541801
hematologic engraftment and immune reconstitution posttransplantation with anti-b1 purged autologous bone marrow.	hematologic engraftment and immune reconstitution were examined in patients who received cyclophosphamide and total body irradiation therapy followed by infusion of autologous bone marrow purged with anti-b1 monoclonal antibody (moab) and complement as therapy for non-hodgkin's lymphoma. hematologic engraftment was prompt with return of greater than or equal to 0.5 x 10(3)/microl granulocytes and greater than or equal to 2 x 10(4)/microl platelets at a median of 26 and 29 days posttransplant, re ...	1987	3542080
analog vs non-analog free t4 assay: effect of sample dilution.		1987	3542293
paraventricular nucleus mediates prolactin secretory responses to restraint stress, ether stress, and 5-hydroxy-l-tryptophan injection in the rat.	the role of the paraventricular nucleus (pvn) in mediating acute stimulatory prl responses was investigated in conscious male rats. electrolytic lesions, verified histologically at autopsy, were stereotaxically made in the pvn region, and sham lesions were made in control rats. blood was obtained through a chronically indwelling catheter in the right atrium. pvn-lesioned (pvl) rats showed significantly lower t3 levels 1 week after surgery (less than 34.4 ng/dl) compared with sham (mean +/- sem, ...	1987	3542515
functions of isolated domains of methionyl-trna synthetase from an extreme thermophile, thermus thermophilus hb8.	methionyl-trna synthetase (metrs, 2 x 75 kda) was purified to homogeneity from an extreme thermophile, thermus thermophilus hb8. the polypeptide chain of metrs was cleaved by limited digestion with trypsin into four domains: t1 (29 kda), t2 (23 kda), t3 (14.5 kda), and t4 (7.5 kda), which were aligned in that order. metrs was also cleaved into similar fragments with a variety of other proteases. domains t1, t2, t3, and t4 were isolated by column chromatography. "tandem domain" t1-t2 (56 kda) is ...	1987	3542990
use of a single thyroxine test to evaluate ambulatory medical patients for suspected hypothyroidism.	previous recommendations for the use of thyroid function tests to diagnose patients with possible hypothyroidism have discounted the value of a total thyroxine (t4) test because many clinically hypothyroid patients have t4 levels that fall within the "normal" range. the authors examined the predictive value of a total t4 measurement in the evaluation of ambulatory general medical patients suspected of having hypothyroidism. pregnant patients and those who were taking medications that interfere w ...	1987	3543264
identification of two new bacteriophage t4 genes that may have roles in transcription and dna replication.	we have identified two bacteriophage t4 genes, 45.1 and 45.2, that map in the intergenic space between phage replication genes 46 (which encodes a recombination initiation protein) and 45 (which encodes a bifunctional protein required in replication and transcription). the existence of genes 45.1 and 45.2 had not been previously recognized by mutation analysis of the t4 genome. we cloned the t4 gene 45.1/45.2 segment, determined its nucleotide sequence, and expressed its two reading frames at hi ...	1987	3543399