Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| contribution to the standardization of influenza neuraminidase inhibition tests, based on enzyme- antienzyme kinetic studies. | 1. from enzyme-antienzyme kinetic studies a method for the determination of anti-n2-neuraminidase antibodies has been developed. this procedure is applicable in routine diagnosis. 2. considering the present conditions, the reversibility of the virus neuraminidase antigen-antineuraminidase antibody complex has been proved. the inhibition must be described as a non-competitive one. 3. as a reference value for comparative immunity estimations the relative antibody concentration t150 is defined as t ... | 1975 | 1126576 |
| kinetics of absorption of toxin of vibrio cholerae. | autoradography of rabbit intestine exposed in vivo to purified tritiated toxin of vibrio cholerae showed that pharmacologically important amounts of toxin can be rapidly absorbed despite the relatively large size of the toxin molecule and its affinity for binding to the brush border. cholera toxin and tritiated nontoxic and tritiated nontoxic control substances of comparable size followed a similar time course in spreading down the surface of the villus, and with respect to the villus greater th ... | 1975 | 1127253 |
| dopamine-like effects of cholera toxin in the central nervous system. | 1975 | 1128681 | |
| comparative immunotherapeutic effect of concanavalin a and neuraminidase-treated cancer cells. | 1975 | 1129845 | |
| modifying the immunogenicity of cell membrane antigens. tumors and transplants. | the immunogenicity of an antigenic cell is a distinctly different property from its antigenic composition. two clinical problems might well be beneficially affected by modifying the immunogenicity of cell membrane antigens: can the immunogenicity of tumor-specific antigens on malignant cells be increased so that effective immunity to the tumor will lead to its destruction? conversely, can the immunogenicity of histocompatibility antigens on grafted organs be minimized so that they will survive a ... | 1975 | 1130323 |
| antitumor immune response following injection of neuraminidase-treated sarcoma cells. | mice inoculated with mca-10 sarcoma cells which had previously been incubated with vibrio cholerae neuraminidase (vcn) demonstrated a significantly lower tumor incidence (9/26) than mice injected with untreated sarcoma cells (10/10) or sarcoma cells incubated with heat-inactivated neuraminidase (28/29) p less than .05. rechallenge of nontumor-bearing mice from the vcn group with untreated sarcoma cells resulted in a low tumor incidence (4/11), indicating that these mice had developed systemic im ... | 1975 | 1130852 |
| rat pancreas adenylate cyclase. iii. its role in pancreatic secretion assessed by means of choleara toxin. | 1. the role of adenylate cyclase in rat pancreas is further investigated by means of cholera toxin, which is known to activate the enzyme in several tissues. 2. cholera toxin activates rat pancreatic adenylate cyclase in vitro upon preincubation of tissue slices with the toxin for more than 30 min, but not when it is merely present during the enzyme assay. the maximal effect is reached after 90 min pre-incubation. the half-maximally activating concentration is 3.5 mu-g/ml upon pre-incubation for ... | 1975 | 1131364 |
| antitumour immunity--1. differential response of neuraminidase-treated and x-irradiated tumour vaccine. | 1975 | 1132396 | |
| isoelectric focusing of isoenzymes of human liver alpha-l-fucosidase. | 1975 | 1132498 | |
| sensitivity of cholera and el tor vibrios to cold shock. | 1975 | 1133577 | |
| radiation-sensitive pyrimidine auxotrophs of ustilago maydis. i. isolation and characterization of mutants. | the relationship between uv sensitivity and pyrimidine auxotrophy has been examined. fourteen pyrimidine-requiring mutants have been classified on the basis of genetic complementation and utilization of biosynthetic intermediates and have been assigned to at least four loci. all the mutants studied were sensitive to uv, although the degree of sensitivity varied both between loci and amongst alleles at the same locus. a double mutant strain carrying pyrimidine mutants at two loci was only as sens ... | 1975 | 1134511 |
| dog immunoglobulins. ii. the antibacterial properties of dog iga, igm and igg antibodies to vibrio cholerae. | secretory iga antibodies to vibrio cholerae were purified from the parotid saliva and mammary secretions of locally and orally immunized dogs using gel filtration, ion-exchange chromatography and anti-immunoglobulin immuno-absorbents. igm and igg antibodies were isolated from serum by gel filtration and ion-exchange chromatography. iga antibodies proved to have minimal, if any, activity in direct killing of bacteria in the presence of complement or in the promotion of phagocytosis. the minimal a ... | 1975 | 1140826 |
| antimicrobial--resistance of vibrio cholerae biotype eltor. | 1975 | 1141793 | |
| sialyl residues in hepatitis b antigen: their role in determining the life span of the antigen in serum and in eliciting an immunological response. | hepatitis b surface antigen was adsorbed to insolubilized sialic acid-specific haemagglutinin isolated from the haemolymph of limulus polyphemus. treatment of the antigen with vibrio cholerae neuraminidase (ec 3.2.1.18) resulted in the release of sialic acid and in an increase of the isoelectric point from ph 4-35 (for subtype ad) or 4-9 (for subtype ay) to ph 5-45. treated, but not untreated, antigen incorporated [14-c]-sialic acid when incubated at 37 degrees c with sialyl transferase (ec 2.4. ... | 1975 | 1141861 |
| proceedings: cell-wall composition of aerobic cutaneous "diphtherios" organisms. | 1975 | 1142405 | |
| isolation of dna polymerase gamma from an adenovirus 2 dna replication complex. | the major dna polymerase in a nuclear membrane complex that is capable of synthesizing viral dna sequences in vitro has been purified about 900-fold from adenovirus 2-infected kb cells. the enzyme was characterized as belonging to the class of mammalian dna polymerases (dna polymerase gamma) that can utilize poly(a) with oligo(dt) as template primer. | 1975 | 1142477 |
| [cholera (review of current studies)]. | 1975 | 1145318 | |
| alkaline phosphatase isoenzymes of human kidney and urine. | kidney and urinary alkaline phosphatases have been studied by starch gel electrophoresis. kidney extracts have shown individual variations and the patterns obtained with cortex and medulla have been clearly different. there are in urine and kidney extracts, phosphatases which share similar properties with regard to electrophoretic mobility, non-susceptibility to treatment with neuraminidase and inhibition by l-phenylalanine. | 1975 | 1149249 |
| ageing in vivo and neuraminidase treatment of rabbit erythrocytes: influence on half-life as assessed by 51cr labelling. | old and young rabbit erythrocytes, separated by centrifugation, contained different respective activities of glucose-6-phosphate dehydrogenase, pyruvate kinase and acetylcholinesterase, and different quantities of stromal sialic acid. a systematic study of the survival rate of young and old erythrocytes incubated with different amounts of vibrio cholerae neuraminidase is described. the half-life of intact old erythrocytes is significantly shorter than that of young erythrocytes with a similar si ... | 1975 | 1150154 |
| letter: isolation of vibrio cholerae. | 1975 | 1150893 | |
| cyanogen condensations as a route to 3-amino-2-imino-1,3-benzothiazin-4-ones with cns depressant potential. | seven new 3-amino-2-imino-1,3-benzothiazin-4-ones, prepared from the condensation of cyanogen and 2-mercaptobenzhydrazides, were evaluated in a neuropharmacological mouse profile. cns depression was observed in several members of the class. | 1975 | 1151723 |
| relationship of brain morphine levels to analgesic activity in acutely treated mice and rats and in pellet implanted mice. | the relationship of brain morphine concentration, determined fluorometrically, to tail-flick activity was investigated after acute and chronic morphine treatment of mice and acute treatment of rats. brain morphine levels were quantitatively related to the analgesic effect on acute administration, with levels of 100 and 140 ng/g of tissue corresponding to the ed50 in mice and rats, respectively. over a 90-minute time course after acute s.c. injection, the analgesic effect of morphine in the tail- ... | 1975 | 1151736 |
| comparative antihypertensive effects and tissue distribution of beta adrenergic blocking drugs. | the precise mechanism of the antihypertensive action of beta adrenergic blocking drugs is not known. both peripheral and central sites of action have been proposed. the comparative antihypertensive actions and tissue distribution of propranolol, pindolol and sotalol were investigated in both normotensive sprague-dawley and spontaneously hypertensive rats. from recordings of concurrent changes in blood pressure and heart rate it was observed that oral or subcutaneous administrations of both propr ... | 1975 | 1151751 |
| effects of nerve growth factors from mouse salivary glands and snake venom on the sympathetic ganglia of neonatal and developing mice. | 1. quantitative histological analysis has been made of the effects of nerve growth factor (ngf) from mouse submaxillary gland and from the venom of vipera russelli on superior cervical ganglia of neonatal mice. 2. the hypertrophic and hyperplastic effects reported by other workers have been confirmed. 3. the hypertrophic effect arises from an increase in the rate at which the sympathetic neurones attain their mature size. the size at maturity is never exceeded. 4. the hyperplastic effect arises ... | 1975 | 1151776 |
| the effect of calcium ions on the binomial statistic parameters which control acetylcholine release at synapses in striated muscle. | a study has been made of the effects of changing [ca]o and [mg]o on the binomial statistic parameters p and n which control the average quantal content (m) of the synaptic potential due to acetylcholine release. 2. when [ca]o was varied in the range 0-1 to 1-0 mm, p increased as the first power of [ca]o whereas n increased as the third power of [ca]o. 3. increasing [mg]o depressed both p and n, however variations of [ca]o in the presence of high [mg]o did not significantly change the power relat ... | 1975 | 1151780 |
| convergence in a thermal afferent pathway in the rat. | 1. in anaesthetized rats, unit activity was recorded in the afferent somatosensory pathway leading from the scrotum. recording sites were in the dorsal horn near the entry zone of the scrotal nerve, in the ventrobasal complex of the thalamus and in the somatosensory (si) cortex. during recording, the temperatures of the left and right sides of the scrotum were varied independently. 2. almost all (64/67) the units in dorsal horn, thalamus and cortex responding specifically to scrotal temperature ... | 1975 | 1151788 |
| proceedings: effects of histamine, 2-methylhistamine and 4-methylhistamine on blood pressure and vascular resistance in the cat. | 1975 | 1151865 | |
| human monocyte metabolism: male vs. female. | 1975 | 1151943 | |
| femoral percutaneous catheterization in infants and small children for cerebral angiography. | an improved technique of selective cerebral angiography for infants and small chicken is described. the needle is inserted approximately 45 degrees to the skin in order to avoid subintimal placement of the tip. after good backflow is established, the guidewire is advanced to a position in the descending aorta. a no. 3 polyethylene catheter and 21-gauge scalp vein needle were most successful. | 1975 | 1153751 |
| further evidence for cross-linking as a protective factor in experimental cholera: properties of antibody fragments. | enzymic fragments of igg antibody were tested for their protective abilities in the infant mouse cholera model. f(ab')2 retained the full protective activity of the parent igg molecule despite losses in complement fixation and opsonic functions. fab' and fab fragments also contained significant protective activity but at a level of only 10% of the intact igg or f(ab')2. self-recombinant univalent f(ab')2 also contained about 10% of the protective activity of the divalent f(ab')2 parent molecule. ... | 1975 | 1159322 |
| antibody cross-linking as a factor in immunity to cholera in infant mice. | several antibody preparations were tested for their ability to reduce adsorption of vibrio cholerae to isolated intestinal epithelial cells, and this ability was related to agglutination and protective activity in infant mice. the results demonstrate that (1) the reduction in adsorption of v. cholerae to epithelial cells correlates with the degree of agglutination for given antibody preparation; (2) intact tantibodies protect infant mice from cholera only at concentrations that agglutinate the b ... | 1975 | 1159323 |
| proceedings: observations on the cholera enterotoxin (choleragen). | 1975 | 1160198 | |
| [effect of infant foods enrichment with iron salts on hemoglobin and hematocrit values and serum iron levels in young infants]. | 1975 | 1160233 | |
| dietary fats and properties of endoplasmic reticulum: i. dietary lipid induced changes in composition of microsomal membranes in liver and gastroduodenal mucosa of rat. | rats were fed for four weeks with different lipid diets to determine the effects on the endoplasmic reticulum membranes of the liver and on the postmitochondrial supernatant fraction of the gastroduodenal mucosa. the diets contained cholesterol, cacao butter, olive oil, and these in combination. the results showed that dietary lipids were able to modify the composition of the hepatic endoplasmic reticulum and, to a lesser extent, that of postmitochondrial fraction of gastroduodenal mucosa. cacao ... | 1975 | 1160521 |
| interrelationship between configurational state and metabolism in mitochondria. | 1975 | 1163126 | |
| socio-economic and environmental factors and human health example of cholera el tor in manila. | the environmental health factors relevant to the occurrence of cholera in an urban milieu (manila) have been studied. cholera has been found to be a disease significantly more prevalent in the areas with lowest environmental condition. association have been found between the disease incidence and several socioeconomic variables: population density, water and sewer connections each per 1000 population and squatter/slum dweller density. the correlation between cholera incidence and population dens ... | 1975 | 1163167 |
| antibacterial mechanisms in the intestine. elimination of v. cholerae from the gastrointestinal tract of adult mice. | 1975 | 1164261 | |
| antibacterial mechanisms in the intestine. elimination of v. cholerae from the intestines of infant mice and the role of antibody. | 1975 | 1164262 | |
| binding of cholera toxin by various tissues. | under certain conditions, it is possible to confirm the observation by peterson (1974) that the cholera toxin-binding capacities of tissues from brain and colon mucosa, and from liver and small intestine mucosa, are comparable. binding of toxin by all tissues except brain is very variable, but is roughtly proportional to their content of the toxin-binding ganglioside galactosyl-n-acetylgalactosaminyl (sialosyl) lactosyl ceramide. it appears that some toxin-binding sites of the mucosa of the smal ... | 1975 | 1165119 |
| comparative morphology of genital cones of genus ancylostoma dubini, 1843. | male worms of ancylostoma braziliense, a. ceylanicum, a. kusimaense, a. malayanum, a. duodenale, a. caninum, a. tubaeforme, agriostomum vryburgi and cyclodontostomum purvisi have the external appendages beside the anogenital aperture. these anogenital structures are morphologically similar and are assumed to be homologous among the species with three pairs of teeth or more. in hookworms with two pairs of teeth, the anogenital structures are complex. anogenital features can be used as a taxonomic ... | 1975 | 1166351 |
| specific effect of neuraminidase on blastogenic response of sensitized lymphocytes. | the blastogenic response of sensitized lymphocytes from guinea-pigs to 'de novo' antigens (klh, hch and ppd) was ehhanced by bcn treatment in twenty-one of twenty-three instances. in contrast, no effect of vcn on nonsensitized guinea-pig lymphocyte response to these antigens, or to mumps antigen, was noted in any of thirty-four instances, these findings indicate that the enhancement effect of vcn is specific for sensitized lymphocytes. heating vcn at 100 degrees for 10 minutes completely abolish ... | 1975 | 1168168 |
| in vitro depression of cellular immunity by friend virus leukemic spleen cells. | 1975 | 1168547 | |
| preparation of high-quality iodine-125-labelled pituitary luteinizing hormone for radioimmunoassay. | high quality pituitary luteinizing hormone labelled with 125-i was obtained after separating out the more heavily iodinated fractions, through starch gel electrophoresis, using the cathodal component (fraction 1) which was further purified on sephadex g-100, giving an almost pure 125-i-labelled luteinizing hormone preparation, presenting excellent immunoreactivity and low levels of damage on incubation in plasma. the quality control of the steps of the technique was done with plasma-coated talc ... | 1975 | 1168555 |
| combined staining of protein-bound sulphydryl groups and dna in polyacrylamide model systems. | a model system of polyacrylamide films containing protein and dna has been used to examine the feasibility of combining the dihydroxydinaphthyldisulphide (ddd)-diazonium salt procedure for localizing protein-bound sulphydryl groups with the feulgen technique for dna to make possible the direct measurement of both these parameters simultaneously. optimun conditions for the sulphydryl group reaction require reduction of the protein-containing films in 10% aqueous ammonium sulphide for 3 hr at 50 d ... | 1975 | 1168630 |
| transport and storage of serotonin by thrombin-treated platelets. | repeated thrombin treatment of washed platelets prepared from rabbits can decrease the serotonin content of the platelets by about 80%. when these platelets are deaggregated they reaccumulate serotonin but their storage capacity for serotonin is reduced by about 60%. if thrombin-pretreated platelets are allowed to equilibrate with a high concentration of serotonin (123 mu m), they release a smaller percentage of their total serotonin upon further thrombin treatment, in comparison with the percen ... | 1975 | 1168649 |
| motility of the oviduct and uterus of the cow during the oestrous cycle. | recordings of electrical activity of the oviduct and uterus were obtained during three oestrous cycles in cows fitted with an extra-cellular multi-electrode assembly. the stages of the cycle were identified by the appearance of the cervico-vaginal secretions and changes in the peripheral plasma level of progesterone were determined by radioimmunoassay. a gradual transition from local non-propagating electrical activity to propagating electrical activity with increase in the duration of contracti ... | 1975 | 1168710 |
| hl-a w27 in psoriatic arthropathy. | forty subjects with psoriatic arthritis (pa) underwent hl-a tissue typing. hl-a w27 was significantly increased in psoriatic spondylitis (35%), whereas statistical significance was not reached in patients with peripheral arthritis alone, although w27 was present in a frequency greater than in controls. psoriatic spondylitis (often asymptomatic) occurred in 57% (23 of 40) of our pa patients. of interest, psoriatic spondylitis was more often w27 negative than w27 positive; nevertheless, w27-positi ... | 1975 | 1169069 |
| on the role of sialic acid in the rheological properties of mucus. | the importance of sialic acid in the rheological properties of mucus has been investigated. both bovine cervical mucus, which is a gel, and the structural glycoprotein derived from it were studied before and after treatment with neuraminidase which selectively cleaves terminal sialic acid residues. the storage modulus, viscosity and circular dichroism spectrum were all essentially changed after removal of the sialic acid. these results would indicate that removal of sialic acid does not affect t ... | 1975 | 1169073 |
| [the effect of neuraminidase on the corpus luteum of the pregnant mouse. an ultrastructural study]. | 1975 | 1170110 | |
| cell contact-dependent ganglioside changes in mouse 3t3 gibroblasts and a suppressed sialidase activity on cell contact. | certain enzyme activities for synthesis and degradation of gangliosides and the chemical quantity and incorporation of radioactivity from [14c] galactose into gangliosides have been studied in 3t3 cells and their transformed counterparts at various cell population densities. the chemical quantity of and the incorporation of radioactivity into gd1a ganglioside increased at the early stage of cell contact ("contact response" of ganglisoide), whereas response was not detectable in transformed 3t3 c ... | 1975 | 1170879 |
| relation of the neuraminidase-revealed antigens of human lymphocytes and erythrocytes. | 1975 | 1171544 | |
| interaction of furazolidone with dna. | dn forms a complex with furazolidone producing thereby a quenching and a bathochromic shift of the drug absorption pattern. the binding isotherm was a non-linear one indicating involvement of more than one binding process in the formation of the furazolidone - dna complex. the furazolidone - dna complex inhibited digestion of dna by dnaase and stabilized dna against thermal strand separation by a significant degree. | 1975 | 1174533 |
| metabolic actions of vasopressin, glucagon and adrenalin in the intact rat. | metabolic effects of vasopressin, glucagan and adrenalin were compared, in intact rats, especially in regard to time courses of effects. hyperglycaemia was transient in response to vasopressin, prolonged following adrenalin, and, suprisingly, was not discernible after glucagon, except in response to a very large dose. vasopressin decreased and adrenalin increased, the plasma free fatty acid concentration; both hormones decreased the triacylglycerol level. muscle glycogen concentrations, measured ... | 1975 | 1182200 |
| cholera toxin effects on cell growth accompanied by selective alterations in metabolite uptake and modification of cell surface proteins. | exposure of chinese hamster ovary cells to cholera toxin at or below mug levels causes a marked morphological changes and increased adhesion and orientation of the cells. such changes are paralleled by alterations in surface proteins as indicated by the cholera toxin-mediated modifications detectable by lactoperoxidase-catalyzed radioiodination of outer proteins. mild tryptic treatment of cells prelabeled with [3h]glucosamine revealed a different kinetics of release of external glycoproteins in ... | 1975 | 1182696 |
| the organized distribution of acrosomal proteinase within the acrosomes of rabbit spermatozoa. | acrosomal proteinase was found to be present in a highly organized distribution in the acrosomes of rabbit spermatozoa, using cytochemistry. this distribution consists of at least six linear loops of evenly spaced proteinase granules, which run diagonally across the flat side of the sperm head in a criss-crossing pattern. two additional loops may be present, one encircling the tip of the spermatozoon in the region of the acrosome reaction, and the other at the posterior end of the acrosome. the ... | 1975 | 1183631 |
| viability of vibrio cholerae biotype el tor and of cholera phage on vegetables. | different vegetables were contaminated with sewage water containing large amounts of el tor vibrios, and the viability of these vibrios was studied under defined conditions of storage. during the dry season (summer) in jerusalem, the el tor vibrios survived for up to 24 hr on parsley, 24 to 30 hr on tomatoes and carrots, 24 to 48 hr on cucumbers, peppers and okra and two to three days on lettuce, when the vegetables were stored at room temperature. the survival time was longer during the rainy s ... | 1975 | 1184361 |
| metabolism of [5-t]fructose by isolated liver cells. | glucose formed from [5-t]fructose in rat hepatocytes contains about 10 to 30% tritium. this does not appear to be due to fructose metabolism via hexokinase since neither the initial presence of glucose, nor wide variations in the original fructose concentration, have much effect on the relative labeling of glucose versus water from [5-t]fructose. comparison of the t:14c ratios in glucose produced from [u-14c, 5-t]fructose and d-[u-14c, 2-t]glyceraldehyde indicate that there is tritium retention ... | 1975 | 1184583 |
| effects of cholera toxin on adenylate cyclase. studies with guanylylimidodiphosphate. | similarities exist between the properties of adenylate cyclase after stimulation by cholera toxin and after stimulation by guanylylimidodiphosphate (gpp-(nh)p). thus a strong stimulation is achieved by both agents, the stimulation is essentially irreversible, the action of certain hormones is enhanced and the enzyme can be solublized with lubrol px in the activated state. because of these similarities the interaction of cholera toxin and gpp(nh)p on adenylate cyclase was examined. it was found t ... | 1975 | 1184756 |
| microbiological examination of ship sewage for the presence of vibrio cholerae. | 1975 | 1191809 | |
| pharmacodynamic action of antygen vi. | 1975 | 1191811 | |
| selective inhibition of cholera toxin- and catecholamine-stimulated lipolysis by blocking agents. | vibrio cholerae enterotoxin stimulates lipolysis in rat epididymal fat cell suspensions. like hormones this toxin increases adenylate cyclase activity, raising levels of cyclic adenosine 3',5'-monophosphate (camp), which activates a cellular lipase. using specific blocking agents, we studied the responses to the adrenergic lipolytic hormones epinephrine, norepinephrine, and isoproterenol, and to cholera toxin. all stimulators were used at 100 x threshold dose. propranolol (34 mum), a beta blocki ... | 1975 | 1193734 |
| laryngeal structure following microcauterization. | the paper supports and reaffirms the objectives of contemporary laryngology in the treatment of cancer of this organ. these objectives are, first and foremost, the eradication of the tumor, and second, the preservation of function. a relatively recent contribution to the "state of the art" has been the development of the field of microlaryngology. the introduction of the surgical microscope has provided the laryngologist with better means to judge the type, localization and extent of neoplastic ... | 1975 | 1195967 |
| comparative in vivo distribution of opiate agonists and antagonists by means of double isotope techniques. | 1975 | 1196002 | |
| synchronization of the fission yeast schizosaccharomyces pombe using heat shocks. | 1975 | 1196141 | |
| pharmacological investigations on elaeocarpus ganitrus. | 1975 | 1197422 | |
| immunotherapy with neuraminidase-treated tumor cells after radiotherapy. | 1975 | 1197655 | |
| [influence of qualitatively different diets on the reproductive capacity and function of the adrenal cortex in rats with experimental toxic hepatitis]. | the effect of a diet rich in protein and the group b vitamins on the function of the ovaries and adrenal cortex, as well as on the reproductive capacity of animals with experimental affection of the liver produced by administration of carbon tetrachloride was studied on 115 mongrel albino female-rats. a high-standard semisynthetic diet devised at the institute of nutrition of the ams of the ussr and three isocaloric experimental diets calorifically enriched by 20, 50 and 100 per cent and contain ... | 1975 | 1199012 |
| [changes in the lungs of white mice under the action of cholera exotoxin]. | 1975 | 1199577 | |
| isolation of single cell nuclei from human epidermis for cytophotometric dna--measurements. | diluted acetic acid was applied to dissociate human epidermis into its constituents. a simple procedure to gain isolated single cells for cytophometric determination of their nuclear dna content is described. the method is suited for single cell cytophotometric measurements and for cytofluorimetric flow systems likewise. the losses of feulgen--stainable nuclear dna during preparation were studied in dependence on the incubation time of the cells in acetic acid. the kinetics of extraction of nucl ... | 1975 | 1200706 |
| location and the primary structure around the disulfide bonds in cholera toxin. | 1975 | 1201095 | |
| dermo--epidermal adhesion and its effect on epidermal structure in the mouse. | evidence is presented that dermo--epidermal adhesion is low in mouse ear. consequently the pressure caused by mitotic activity in the basal epidermal layer is readily relieved by cell extrusion distally and the number of basal cells per unit skin area remains low. the rule then is that the number of distal cells per unit skin area must be equally low, and the epidermis is therefore thin. conversely, dermo--epidermal adhesion is high in mouse sole-of-foot epidermis, and the mitotic pressure leads ... | 1975 | 1201191 |
| an improved method of assessing topical corticosteroid activity. | topical application of ointment bases causes varying degrees of epidermal thickening in guinea-pigs. this is reproducible and can be accurately measured. suppression of these changes was produced by addition of beta-methasone 17-valerate, fluocinolone acetonide, fluocinonide and hydrocortisone acetate. the inhibitory effect of these steroids was related to the type of corticosteriod, its concentration and the ointment base. the effect was still evident at extremely high dilutions of the steroids ... | 1975 | 1201192 |
| pure red cell aplasia: studies on an igg serum inhibitor neutralizing erythropoietin. | a new type of igg serum inhibitor in adult pure red cell aplasia (prca) has been investigated. this inhibitor is directed against circulating erythropoietin (ep) (prca type b), rather than the erythroid marrow (prca type a). thus, the igg inhibitor, after interaction with ep in solution, is precipitated together with ep by addition of goat anti-human gamma-globulins. pre-therapy prca serum, although apparently devoid of ep, shows considerable ep activity following acidification and boiling. the ... | 1975 | 1201224 |
| binding of vitamin b12--rat transcobalamin ii and free vitamin b12 to plasma membranes isolated from rat liver. | when dialysed rat serum which contains a single, low molecular weight binder for vitamin b12, rat transcobalamin ii (rat tc-ii), was labelled in vitro with 57co-vitamin b12 and then incubated at 30 degrees c (ph 7-5) with vesicles of highly purified plasma membranes separated from microsomal fractions of rat liver by density gradient centrifugation, the 57co-vitamin b12-rat tc-ii complex bound to high affinity sites on the vesicles via a specific (binding after correction for 'non-specific' bind ... | 1975 | 1201244 |
| ultrastructural studies of the bone marrow in sickle cell anaemia. ii. the morphology of erythropoietic cells and their response to deoxygenation in vitro. | electron microscopic studies of bone marrow aspirates obtained from patients with homozygous sickle cell anaemia (hbss) were fixed immediately without attempts to deoxygenate the samples. erythroblasts and normoblasts in these preparations were devoid of haemoglobin polymers or other indications of sickling. furthermore, the nucleated erythroid cells from sickle-cell patients presented an ultrastructural morphology indistinguishable from that of identically-processed erythroid cells in marrow sa ... | 1975 | 1201249 |
| comparative study of the smooth muscle contractor activity of airborne dusts and of dustiness in cotton, flax, and jute mills. | a bioassay technique using isolated guinea-pig ileum was employed to compare the smooth muscle contractor activity of various dusts from mills in which the prevalence of byssinosis was known. the activity of dust from a mill spinning a coarse grade of cotton was several times greater than that in dust from a mill processing a fine grade of cotton. there was a similar order in the difference of the prevalence of byssinosis in these mills. however, the activities of fine cotton, flax, and jute dus ... | 1975 | 1201255 |
| immunoglobulin synthesis and antibody content in the small intestine of the rabbit. | the concentration of immunoglobulin and specific antibody in the serum and the intestinal fluid and the rate of synthesis of immunoglobulin in the small intestine was measured in normal and immunized rabbits. iga was found to be the predominant immunoglobulin in the intestinal fluid. iga and igg were secreted at rates of 4-3 mug/cm/hr and 1-3 mug/cm/hr respectively. specific anti-vibrio cholerae antibodies in the intestine were found mainly in the iga class after oral immunization. | 1975 | 1204251 |
| pinocytic activity in neuraminidase treated ameba. | 1975 | 1205516 | |
| comparison of the rate of absorption and proteolysis of [14c]choleragen and [14c]bovine serum albumin in the rat jejunum. | [14c]choleragen was used to study the rate of disappearance of choleragen enterotoxin from the jejunum of rats. [14c]bovine serum albumin (bsa) was studied in a similar manner. almost one-third of the labeled toxin had disappeared from the intestine after 6 h. its rate of disappearance was the same in germfree rats as in conventional rats. the rate of proteolysis of [14c]choleragen and [14c]bsa by intestinal mucodal lysosomal enzymes was also studied. neither was significantly degraded by neutra ... | 1975 | 1205622 |
| rapid and reliable preparation of macroaggregated albumin suitable for lung scintigraphy. | 1975 | 1205665 | |
| protein reagent modification of cholera toxin: characterization of effects on antigenic, receptor-binding and toxic properties. | the effects of protein modification procedures on the biologically most important properties of cholera toxin, i.e. the toxic activity, the gm1 receptor-binding capacity and the antigenic (antibody-fixing) properties, have been studied quantitatively using microgram amounts or less of toxin protein. most of the 24 group-specific reagents used had either no inhibitory effect on the toxic or the combination of gm1-binding and antibody-fixing properties of cholera toxin, or they had a concomitant i ... | 1975 | 1206372 |
| growth inhibition of vibrio cholerae by d-camphor. | 1975 | 1206376 | |
| abortifacient effects of vibrio cholerae exo-enterotoxin and endotoxin in mice. | to study antifertility properties of microbial toxins, exoenterotoxin and endotoxin from vibrio cholerae were injected intravenously into mice at different times during pregnancy. the two substances induced termination of pregnancy, but the patterns of abortifacient activity were different. exotoxin terminated pregnancy in mice when administered between days 4 and 10 of gestation, but abortifacient activity was reduced in animals more than 10 days pregnant; exogenous progesterone did not protect ... | 1975 | 1206628 |
| [utilization of serological methods in the retrospective diagnosis of cholera and in detecting vibrion carriers]. | sensitivity and specificity of the three serological methods were studied comparatively: the vibriocidal test, the reaction of bacterial agglutination and of indirect hemagglutination, with the use of erythrocytes sensitized with the vibrio lyzate, cholera species o-antigen and cholerogen. investigations were conducted with the blood sera of cholera patients, vibrio carriers and contacts. vibriocidal test proved to be the most sensitive; its data correlated with the results of bacterial agglutin ... | 1975 | 1210898 |
| [role of neuraminidase from cholera vibrions in the pathogenesis of experimental cholera]. | a study was made of a possible effect of neuraminidase of cholera vibrios on cholera pathogenesis. it was shown that in intraintestinal injection of cholera vibrios of the el tor biotype to nursling rabbits neuraminidase could be revealed in their intestine 5 to 8 hours after the infection. addition of neuraminidase to the weakly cholerogenic strain cholera vibrios intensified its cholerogenic action in infection of the animals. the antineuraminidase serum administered to the infected rabbits pr ... | 1975 | 1210912 |
| a salmonella california contamination of a turkey feed concentrate. | 1975 | 1212606 | |
| selective chemical changes of gonadotropic hormones by means of neuraminidase from vibrio cholerae. | experiments indicate that neuraminidase from vibrio cholerae selectively cleaves sialic acid derivate from fsh, but leaves the lh sialic acid derivative. by performing a specific test for lh - the oaad test - we found that the lh activity is not influenced by the presence of neuraminidase. the fsh activity is destroyed by neuraminidase but the enzyme itself influences the ovarian weight, resulting in a weight decrease observed 48 hours after injection. before or after this "critical time" no dec ... | 1975 | 1212991 |
| [comparative study of the epidemiology of cholera and sensitivity to antibiotics of strains collected during the epidemiological period in 1971 and 1972 in wilaya de constantine]. | 1975 | 1213515 | |
| studies on the so-called nag vibrios. | 1975 | 1213771 | |
| studies on bacteriology of cholera infection in varanasi. | 1975 | 1213804 | |
| [changes in the composition of intracellular lipids and hydrocarbons in candida tropicalis in the absence of growth]. | the fatty acid composition of lipids and the composition of hydrocarbons were studied in candida tropicalis cultivated on a medium with propionic acid and incubated in the conditions of starvation and on a medium containing glucose-1-6-14c but no nitrogen. intracellular fatty acids with an odd number of carbon atoms were found to be easily metabolized by yeast cells. the content and composition of intracellular hydrocarbons were very labile and underwent intensive transformation caused by change ... | 1975 | 1214607 |
| [antimicrobic properties of facultative anaerobes isolated from oil-bearing wells]. | 1975 | 1214635 | |
| [formation of fatty acids by aspergillus ochraceus 15b growing on a hexadecane containing medium]. | 1975 | 1214637 | |
| [electron microscopic study of double infection induced by potato virus x and potato virus y in nicotiana glutinosa]. | 1975 | 1214639 | |
| [salmonellosis carrier state]. | 1975 | 1214656 | |
| [electron microscopy of cholera vibrios from the intestinal contents of infected suckling rabbits]. | electron microscopic study of the cells of classic, el tor and nag-vibrios showed them to be no different by the structure type from the cells of ofter gram-negative bacteria. a characteristic peculiarity of the cholera vibrios revealed after their passage through the intestine of nursling rabbits was the presence of microcapsules and protrusions of the areas of the wall membranous apparatus. | 1975 | 1217357 |
| recent studies on genetic recombination in vibrio cholerae. | 1975 | 1219899 | |
| interbiotype conversion of cholera vibrios by action of mutagens. | 1975 | 1219900 | |
| the antibody basis of local immunity to experimental cholera infection in the rabbit ileal loop. | 1975 | 1219901 | |
| bacteriology at the periphery of the cholera pandemic. | 1975 | 1219904 |