Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [changes in endocrine status in cancer patients and the effect of chemotherapy on endocrine functions]. | changes in serum growth hormone (gh) concentrations before and after trh injection, oral glucose tolerance test (ogtt), l-dopa, cb-154 and lhrh administration were investigated in 78 gastric, 32 hepatocellular, 23 colonic, 20 pulmonary, 11 esophageal, 9 pancreatic and 9 other types of cancer patients. in addition, the effect of 5-fu or tegafur on thyroid function was studied in 39 gastric, 12 colonic, 8 hepatocellular, 5 pancreatic and 8 other types of cancer patients. the results obtained were ... | 1987 | 2436576 |
| serum hormones and seminal parameters in males with thyroid disturbance. | semen characteristics and serum levels of the hormones t3, t4, ft4, tsh, fsh, lh, prolactin and testosterone have been measured in five men with thyroid disorders (two patients had primary hypothyroidism, two had graves disease and one had subacute thyroiditis). alteration in thyroid status negatively affects semen quality, more markedly in hypothyroidism than in hyperthyroidism. treatment of patients improves the quality of semen. | 1987 | 2446532 |
| immunosuppressive effects of isoprinosine in man: a comparison to chlorambucil effects in multiple sclerosis. | immunological and clinical functions were studied over a 2 year period in conjunction with a placebo controlled trial of isoprinosine and chlorambucil in 21 patients with exacerbating remitting multiple sclerosis. laboratory and clinical evaluations were performed at 3 month intervals and during relapses. in placebo-treated patients, the decrease in circulating t8+ cells was maximum during relapses, t lymphocyte function was impaired, and five of the six patients experienced clinical worsening. ... | 1987 | 2446758 |
| production and detection of coliphage t4 endonuclease v polyclonal and monoclonal antibodies using staphylococcal protein-a hybrid proteins. | to facilitate the production of antibodies against endonuclease v, a pyrimidine dimer-specific dna glycosylase produced in bacteriophage t4-infected escherichia coli, we constructed plasmids containing protein-a-endonuclease v fusion genes under control of the e. coli tac promoter. induction with isopropyl-beta-d-thiogalactopyranoside produced large amounts of fusion proteins, which could easily be purified on human igg agarose columns. the affinity-purified fusion proteins were injected into ra ... | 1987 | 2446955 |
| chronic relapsing course of encephalomyeloradiculopathy in a 6-year-old boy. | we present a young child with an illness characterized by remissions and exacerbations and signs of disseminated lesions in the central nervous system. visual evoked response and brainstem auditory evoked potential gave abnormal findings showing lesions also of the optic nerves and brainstem. the csf igg index was slightly elevated and myelin basic protein was high. there was also intrathecal antibody production against different viruses, the highest against measles. the hla type was a3, b7, dr2 ... | 1987 | 2447517 |
| effects of oral erythrosine (2',4',5',7'-tetraiodofluorescein) on thyroid function in normal men. | erythrosine (er), a tetraiodinated derivative of fluorescein, is a coloring agent widely used in foods, cosmetics, and pharmaceutical products. because of its high iodine content and previous reports demonstrating an inhibitory effect of erythrosine on hepatic 5'-monodeiodination, we studied the effects of this compound on thyroid function and serum and urinary iodide concentrations in normal subjects. thirty normal men, equally divided into three treatment groups, each received a 14-day course ... | 1987 | 2447681 |
| [demonstration and ontogenesis in the rat of a serum protein analogous to human thyroxine binding globulin]. | it has been reported evidence based on equilibrium binding, electrophoretic, immunoelectrophoretic studies, that the rat possesses a major high affinity thyroid hormone binding protein, with an electrophoretic mobility and binding properties similar to those of the human thyroxine binding globulin (tbg). it is shown that in the sera of postnatal developing animals, between 3 and 21 days, the thyroxine (t4) and the triiodothyronine (t3) binding activities increase up to 10 times over adult or foe ... | 1987 | 2448017 |
| preparation and separation of the glucuronide and sulfate conjugates of thyroxine and triiodothyronine. | an enzymatic method for synthesis of labelled thyroxine glucuronide (t4g) and triiodothyronine glucuronide (t3g) from labelled thyroxine (t4) and triiodothyronine (t3) is presented. the synthetic glucuronides are completely digested by beta-glucuronidase, with recovery of the parent t4 or t3. they have distinctive elution patterns on hplc and on sephadex g25 chromatography, and can be clearly separated from t4 and t3 as well as from synthetic t4 sulfate (t4s) and t3 sulfate (t3s). on lh 20 chrom ... | 1987 | 2448134 |
| monoclonal antibodies to human thyroglobulin as probes for thyroglobulin structure. | a panel of monoclonal antibodies (mabs) against human thyroglobulin (htg) was obtained by somatic fusion of the nonsecreting myeloma cell line p3x66 ag8/0 and spleen cells of balb/c mice immunized with purified htg. antibody secreting clones were selected by solid phase enzyme immunoassay and analyzed for cross-reaction with tg from several animal species. twelve out of 15 mabs cross-reacted with both rat and mouse tg and 11 mabs cross-reacted with bovine tg. the cross-reaction with mouse tg par ... | 1987 | 2433129 |
| a serine proteinase inhibitor produced by an htlv i virus-transformed human t lymphocyte line. | a previous report from our laboratory indicated that a proteinase inhibitor is produced by rabbit t lymphocytes. we now report that a human t cell line, c91/pl, produces a proteinase inhibitor which inhibits the enzymatic activity of trypsin and kallikrein. this newly identified proteinase inhibitor (lpi 1) did not inhibit the enzymatic activity of four other serine proteinases (thrombin, plasmin, chymotrypsin, or pancreatic elastase), a thiol proteinase (papain), or a carboxyl proteinase (pepsi ... | 1987 | 2433346 |
| the t4 epitope is not required for a normal replicative cycle of human immunodeficiency virus. | 1987 | 2433359 | |
| human t clones reactive to the sexual stages of plasmodium falciparum malaria. high frequency of gamete-reactive t cells in peripheral blood from nonexposed donors. | malarial gametocytes, which are taken up by mosquitoes during a blood meal, develop in the gut of the mosquito into gametes. gametes and gametocytes contain the target antigens of transmission-blocking immunity. here, we show that the peripheral blood of nonexposed donors contains plasmodium falciparum gamete-reactive t cells at frequencies ranging from 1/300 to 1/4000. studies on long-term clones demonstrated that these cells often recognized antigens shared between gametes and asexual stage pa ... | 1987 | 2431058 |
| abrogation of staphylococcal enterotoxin a-induced suppressor cell activity by the anti-tac monoclonal antibody. | human mononuclear cells stimulated with staphylococcal enterotoxin a (sea) for 2-6 days significantly suppress [3h]thymidine incorporation and reduce the levels of interleukin 2 (il-2) and interferon (ifn) in culture medium when added to fresh, polyclonally activated mononuclear cells. the inhibitory capacity of the cells correlates well with the expression of il-2 receptors. lymphocytes obtained 3 days after stimulation with sea, when the il-2 receptor expression is high, are more potent inhibi ... | 1987 | 2433737 |
| charcoal-dextran treatment of fetal bovine serum removes an inhibitor of human cfu-megakaryocytes. | serum has been shown to have an inhibitory effect on the growth of human megakaryocyte colony-forming units (cfu-meg), both in the methylcellulose and plasma clot systems. charcoal-dextran (cd) treatment of fetal bovine serum (fbs), a method that is known to adsorb free hormones and various other substances from their protein-bound counterparts, has been used for the improvement of human erythroid colony growth. we now report a cd treatment of fbs to improve human cfu-meg growth, using a modifie ... | 1987 | 2434353 |
| histopathology and immunohistology of htlv-iii/lav related lymphadenopathy and aids. | fifty-nine lymph node biopsies from homosexual men with serum antibodies to htlv-iii/lav were the subject of a detailed histopathological and immunohistochemical study. the histological findings were correlated to the patients clinical status, and the t4/t8 ratios in blood and lymph nodes. four histological patterns predominated and were defined as follicular hyperplasia (fh), follicular fragmentation (ff), follicular atrophy (fa), and follicular depletion (fd). immunocytochemical studies indica ... | 1987 | 2435107 |
| developmental therapeutics and the acquired immunodeficiency syndrome. | patients with the acquired immunodeficiency syndrome (aids) die of overwhelming infections as a consequence of the destruction of the t4 subset of lymphocytes. approaches to the treatment of aids have involved attempts to reestablish immune competence as well as treat opportunistic infections. the discovery of the human t-lymphotropic virus type iii, which causes aids, has provided a specific target for screening antiviral drugs. there are many potential screening targets, from surface-binding p ... | 1987 | 2435201 |
| increased density of class i major histocompatibility complex antigens and decreased density of t-cell differentiation antigens in the early stages of t-cell activation. | major histocompatibility complex (mhc) antigens and t-cell differentiation antigens on activated t cells play a central role in t-cell interactions. in the present study, we have analyzed time courses of both quantity and density of the t-cell differentiation antigens, cd3 (t3), cd4 (t4), and cd8 (t8), as well as mhc antigens, on the cell surface of t cells, and made correlated measurements of dna content with the surface antigen quantity as well with rna content and cell size following activati ... | 1987 | 2435684 |
| human mumps virus-specific cytotoxic t lymphocytes: quantitative analysis of hla restriction. | to obtain quantitative information about the use of hla antigens as restriction element by antiviral cytotoxic t lymphocytes (ctl), we have analyzed precursors of human mumps virus-specific ctl by limiting dilution. ctl generated by restimulation of peripheral blood t lymphocytes with autologous mumps virus (mv)-infected stimulator cells were restricted by autologous hla class i antigens, and derived from the t4-8+ population. they were specific for mv and did not lyse autologous target cells in ... | 1987 | 2435685 |
| radiation therapy of anal epidermoid carcinoma. | between 1978 and 1984, 44 consecutive patients with anal epidermoid carcinoma were either given radiation therapy alone (cloacogenic type) or in combination with bleomycin (squamous type). the patients with small tumors (t1-t2) were treated to 65 gy or 60 gy + bleomycin directly, whereas patients with moderately advanced tumors (t3) were treated to the same radiation dose, only if no evidence of residual disease existed after 50-55 gy (40-45 gy + bleomycin); if a palpable tumor still remained 3 ... | 1987 | 2435686 |
| functional analysis of t lymphocyte subsets in antiviral host defense. | the role of different t cell subsets in antiviral host defense was investigated by treating thymectomized c57bl/6 and cba/j mice with monoclonal rat anti-lyt-2 or anti-l3/t4 igg 2b antibodies 14 and 10 days before infection. this treatment depleted the respective t cell subsets to undetectable levels in peripheral blood when assayed by immunofluorescence. in mice treated with anti-lyt-2, induction of cytotoxic t cells was reduced to less than 1 to 2% after intravenous infection with armstrong st ... | 1987 | 2435794 |
| dna synthesis arrest sites at the right terminus of rat long interspersed repeated (line or l1rn) dna family members. | an approximately equal to 150-bp gc-rich (approximately equal to 60%) region is at the right end of rat long interspersed repeated dna (line or l1rn) family members. we report here that one of the dna strands from this region contains several non-palindromic sites that strongly arrest dna synthesis in vitro by the prokaryotic klenow and t4 dna polymerases, the eukaryotic alpha polymerase, and amv reverse transcriptase. the strongest arrest sites are g-rich (approximately equal to 70%) homopurine ... | 1987 | 2436148 |
| immunocompetent cells of fixed drug eruption. | the positive provocation test reactions of the skin of six patients with fixed drug eruption (fde) were studied from timed skin biopsies taken between 2 hours and 9 days after the appearance of fde. monoclonal antibodies to the following immunocompetent cell surface epitopes were used: t3, t4, t6, t8, t9, m1, ia1, drc, leu7 and b cell. the dermal infiltrate comprised 60-80% of t lymphocytes at all the times studied. cells with t4 and t8 epitopes were displayed in similar numbers. a transient dec ... | 1987 | 2436411 |
| [induction chemotherapy of carcinomas of the oral cavity and oropharynx. feasibility study, levels and factors of response to treatment. apropos of 135 cases]. | induction chemotherapy is now frequently included in therapeutic protocol for carcinoma of oral cavity and oropharynx. frequency of objective responses and predictive factors for favorable responses to therapy were evaluated in 135 patients. in 45% of cases an objective tumoral regression was noted (with 6% total regression). combined cddp-5 fu produced responses in 65% of cases, whereas the combinations of cddp-blm-vcr-mtx and of cddp-blm-vds produced values of 35 and 41% respectively. small tu ... | 1987 | 2448863 |
| correlation between systemic lupus erythematosus and t4 epitope phenotype. | three groups of black subjects (systemic lupus erythematosus patients, patients with nonrheumatic disease, and normal subjects) were screened for the expression of the t4 epitope, as recognized by the monoclonal antibody okt4. we found that the t cell subsets within each group were similar, regardless of the t4 epitope phenotype (intact, intermediate, or deficient). in the subgroup of jamaican subjects, there was an association between systemic lupus erythematosus and the t4 epitope-intermediate ... | 1987 | 2449224 |
| studies on b lymphoid tumors treated with monoclonal anti-idiotype antibodies: correlation with clinical responses. | monoclonal anti-idiotype antibodies can be made which are exquisitely specific for b lymphocytic malignancies. we have conducted a clinical trial in which some patients' tumors regressed after infusion of such antibodies. here, we evaluated characteristics of the antibodies, the tumors, and the patients to determine which features best correlated with the clinical response. neither the isotype of the murine antibodies, nor their avidity were predictive of clinical outcome. the specific epitope t ... | 1987 | 2431729 |
| immunologic studies of subjects with asthma exposed to formaldehyde and urea-formaldehyde foam insulation (uffi) off products. | there has been concern in recent years as to the health hazards of exposure to potentially carcinogenic or immunotoxic substances in the environment. this study was done to determine the effects of exposure to urea-formaldehyde foam insulation (uffi) off products on various hematologic and immunologic parameters in subjects with asthma: complete blood count and differential, erythrocyte sedimentation rate, lymphocyte subpopulations (e-rosetting, t3, t4, t8, b73.1, and fc receptor positive lympho ... | 1987 | 2437172 |
| mapping of human apolipoprotein b antigenic determinants. | a minimum of 16 epitopes which provide a group of topographical markers to study the conformation of apolipoprotein (apo) b have been mapped in relation to elements of the sequence of apo b-100. six of these epitopes are identified by monoclonal antibodies (mabs) directed against low density lipoprotein (ldl) apo b, while at least 10 others react with mabs obtained by immunization with delipidated and solubilized apo b. five epitopes which are also expressed on apo b-48 have been assigned to the ... | 1987 | 2437897 |
| carrier rna enhancement of recovery of dna from dilute solutions. | a study of the use of carrier rna to improve precipitation of dna from dilute solutions was conducted to define the conditions which optimize dna recovery. replicate samples containing labeled pbr322 and increasing concentrations of commercially-available torula yeast rna were ethanol precipitated at -20 degrees c for 1 h in microfuge tubes obtained from various manufacturers. nucleic acids were pelleted by centrifugation for either 5 or 30 min, dried and resuspended. although recovery was not i ... | 1987 | 2437909 |
| myelin basic protein and proteolipid protein reactivity of brain- and cerebrospinal fluid-derived t cell clones in multiple sclerosis and postinfectious encephalomyelitis. | t cells were directly cloned from autopsied ms brain plaque tissue and reactivity was measured with the major encephalitogenic neuroantigens, myelin basic protein (mbp), and proteolipid protein (plp). control clones were simultaneously derived from the blood. the proportion of t4+ and t8+ t cell clones from the brain tissue differed from that of peripheral blood t cell clones derived at the same time, suggesting that the clones were not derived from the peripheral blood. none of 57 brain-derived ... | 1987 | 2438352 |
| lunar cycles of coho salmon, oncorhynchus kisutch. ii. scale amino acid uptake, nucleic acids, metabolic reserves and plasma thyroid hormones. | [14c]glycine uptake by scales in vitro was measured in coho salmon (oncorhynchus kisutch walbaum) smolts at different times during several semi-lunar cycles. there was a clear cyclical pattern of glycine uptake during the semi-lunar period. evidence for semi-lunar cycles of liver and muscle rna:dna ratios, carcass water content, haematocrit, and plasma triglyceride, glucose and cholesterol levels was also found in coho salmon parrs. plasma l-thyroxine (t4) levels exhibited a cyclical pattern dur ... | 1987 | 2438366 |
| suppressor and novel mutants of bacteriophage t4 trna(gly). | we have isolated a weak uga suppressor of phage t4 trna(gly) in which the anticodon is changed from ucc to uca. two secondary mutants lacking suppressor activity are atypical in accumulating trna(gly). both mutations change the t stem of the cloverleaf model. one involved a g to a change at the 5' base position of the middle base-pair; the second involves a c to u change at a constant base position next to the t loop. the precursor rnas of the mutants were cleaved in vitro with the catalytic rna ... | 1987 | 2438421 |
| [multidisciplinary treatment of maxillary cancer]. | cases of maxillary cancer treated at our institute can be divided into two groups. in the first group (1971-1982, n = 85), we treated maxillary cancer by preoperative intraarterial infusion of 5-fu and linac x-ray irradiation (60 gy/6 weeks), followed by maxillectomy. in the second group (1982-1986, n = 32), we further combined intraarterial or intravenous chemotherapy using cddp preoperatively or postoperatively depending upon the stage of the cancer. five-year survival rate was 64.7% in the fi ... | 1987 | 2439014 |
| escherichia coli endonuclease iii is not an endonuclease but a beta-elimination catalyst. | the oligonucleotide [5'-32p]pdt8d(-)dtn, containing an apurinic/apyrimidinic (ap) site [d(-)], yields three radioactive products when incubated at alkaline ph: two of them, forming a doublet approximately at the level of pdt8da when analysed by polyacrylamide-gel electrophoresis, are the result of the beta-elimination reaction, whereas the third is pdt8p resulting from beta delta-elimination. the incubation of [5'-32p]pdt8d(-)dtn, hybridized with poly(da), with e. coli endonuclease iii yields tw ... | 1987 | 2439070 |
| humoral and cellular immune abnormalities in adult hemophiliacs followed over a 2-year period. | immune function was assessed in 93 adult hemophiliacs in the 2 years prior to htlv-iii antibody testing of blood donors and routine heat treatment of coagulation factor concentrates. parameters of humoral and cellular immunity studied included serum complements, immunoglobulins, immune complexes, blood cell counts, lymphocyte subsets, lymphocyte transformation, 2-5a synthetase, serology for htlv-iii and hepatitis b, skin tests, and clinical status. htlv-iii seropositivity was significantly more ... | 1987 | 2439226 |
| activation of t cells by cross-linking an anti-cd3 antibody with a second anti-t cell antibody: mechanism and subset-specific activation. | binding of anti-cd3 antibody bma030-f(ab')2 to t cells is not able to induce t cell proliferation even after additional cross-linking by plastic-bound goat anti-mouse ig antibodies (panning) and addition of either interleukin (il) 1 or il2. in search for agents able to complement the signals provided by bma030-f(ab')2, several antibodies directed against cd2, cd4, cd5, cd6 and cd8 were used. neither of these caused t cell proliferation either by themselves or when simply added together with bma0 ... | 1987 | 2439351 |
| photoaffinity labeling of t4 bacteriophage 32 protein. | with a view toward the determination of nucleic acid binding domains and sites on nucleic acid helix-destabilizing (single strand-specific) proteins (hdps), we have studied the interactions of the copolymer polynucleotide photoaffinity label, poly(adenylic, 8-azidoadenylic acid), (poly(a,8-n3a] with the t4 bacteriophage hdp, 32 protein. poly(a,8-n3a) quenched the intrinsic tryptophan fluorescence of 32 protein in a manner similar to that observed with other polynucleotides, and the effect could ... | 1987 | 2439510 |
| absence of okt4 antigen epitope: report of a case in a brazilian population. | during the study of 162 normal subjects we found an 8 month-old black girl without previous illnesses, especially infections, whose lymphocytes did not react with okt4 monoclonal antibody. however the cd4 subset was present, since the number of lymphocytes which reacted with leu 3a and t4 was normal. in addition the percentage of fc mu+ lymphocytes, k cell activity and pha response were normal. the proportion of the other lymphocyte subsets did not differ from normal controls. four first degree ... | 1987 | 2441061 |
| [decreased synthetic activity as a possible cause of the death of escherichia coli bacteria during amino acid starvation]. | the work is concerned with studying the breakdown of proteins and rna when a polyauxotrophic escherichia coli strain is incubated in a salt solution without amino acids, phosphorus, nitrogen and glucose at 43 degrees c as well as the ability of starving bacterial cells to recommence protein and rna synthesis (also in the course of phage t4 infection) and to reproduce bacteriophages t4, lambda and ms2. within the first two hours of the incubation, 12% of proteins and 40% of rna break down to acid ... | 1987 | 2441235 |
| treatment of patients with stage iv cancer: do the ends justify the means? | most experienced head and neck surgeons recommend aggressive treatment--including radical surgery--for patients with resectable stage iv cancers. yet, given the poor overall cure rate of 15% and the deformity and disability often associated with treatment, one of the most frequently asked questions at our conference on tumors was: are we really helping these patients? we found little data in the relevant literature to answer this or other questions. are there subgroups with a better outlook? wha ... | 1987 | 2441340 |
| anticonvulsant medication use and circulating levels of total thyroxine, retinol binding protein, and vitamin a in children with delayed cognitive development. | circulating thyroxine (t4), retinol binding protein (rbp), and vitamin a were measured in conjunction with nutritional status assessment of 707 cognitively delayed children, ages 3.0-9.0 y. twenty percent were receiving anticonvulsant (ac) medication. t4 was lower and rbp and vitamin a were higher (p less than 0.0001) among ac than non-ac subjects. molar ratios of vitamin a:rbp did not differ between the two groups nor did intakes of protein or vitamin a. lower t4 and higher rbp were found among ... | 1987 | 2441590 |
| immunohistology of adenolymphoma (warthin's tumour): evidence for a role of vascularization in the organization of the lympho-epithelial structure. | the epithelial, vascular and lymphoid components present in two cases of adenolymphoma (warthin's tumour) were characterized by immunohistochemistry on frozen sections. epithelial cells were positive for cytokeratin and keratin and were in close contact with numerous factor viii related antigen-positive capillary vessels, running parallel to the epithelial basement membrane. the capillary vessels were in communication with high endothelial post-capillary venules which were often lined by hla-dr- ... | 1987 | 2442085 |
| immunohistology of anaplastic thyroid carcinoma. a study of 43 cases. | forty-three cases of large cell anaplastic thyroid carcinoma were examined with various antisera. four histological patterns were identified: spindle cell, giant cell, trabecular and squamous. in 38 cases the epithelial origin was demonstrated with various epithelial markers: 11 cases stained positively for thyroglobulin, 19 for t3 and/or t4, 35 for fat globule membrane antigens, 28 for keratin, 29 for lactoferrin, and one for calcitonin. five cases were negative for all epithelial markers but c ... | 1987 | 2442086 |
| interrupted thymidylate synthase gene of bacteriophages t2 and t6 and other potential self-splicing introns in the t-even bacteriophages. | southern hybridization analyses of procaryotic dna from escherichia coli, lambda bacteriophage, and t1 to t7 phages were carried out. the hybridization probes used consisted of dna restriction fragments derived from the t4 phage intron-containing thymidylate synthase gene (td) and short synthetic oligodeoxynucleotides defining specific exon and intron regions of the gene. it was shown that intact as well as restricted dna from the t-even phages hybridized not only to both t4 phage td intron- and ... | 1987 | 2442142 |
| immunoelectron microscopic demonstration of thyroglobulin and thyroid hormones in rat thyroid gland. | we have developed a post-embedding immunogold technique for electron microscopic localization and quantitation of thyroglobulin (tg), thyroxine (t4), and triiodothyronine (t3) in rat thyroid. labeling for tg was located on rough endoplasmic reticulum, golgi apparatus, exocytotic vesicles, luminal colloid, colloid droplets, and lysosomes, whereas labeling for thyroid hormones was located on luminal colloid, colloid droplets, and lysosomes. we tested different procedures of fixation, dehydration, ... | 1987 | 2442241 |
| effects of fetal thyroid states on rna, dna, and tubulin content in neonatal rat brain. | 3,5-dimethyl-3'-isopropyl-l-thyronine (dimit)-induced fetal hyperthyroid rats showed a marked accumulation of intracellular tubulin content in the cerebral cortex, hypothalamus, and cerebellum at 1 day old. when the fetuses were transplacentally radio-thyroidectomized with the administration of [131i]-na, the brain weight was not changed at 1 day old. the dna content was not affected by the radio-thyroidectomy (tx), but intracellular rna concentration (per dna) was increased in the hypothalamus ... | 1987 | 2442441 |
| low natural killer syndrome: clinical and immunologic features. | twenty-three patients with low natural killer syndrome (lnks), 7 males and 16 females, are reported here. these lnks patients had an age range from 14 to 77 years, with a median of 36.5 years. lnks is a newly proposed category of immune disorders, being characteristically diagnosed by lowered nk cell activity against k562 target cells as a definite laboratory abnormality, in association with general clinical symptoms of remittent fever and uncomfortable fatigue, persisting without explanation fo ... | 1987 | 2442602 |
| synthesis and application of derivatizable oligonucleotides. | to investigate protein-dna interactions, we have synthesized a versatile phthalimide-protected 5-(3-aminopropyl)-2'-deoxyuridine nucleoside probe. the modified residue was incorporated into deoxyoligonucleotides by automated synthesis. the standard oligonucleotide workup also exposed the pendent amino group, which was found to react with either fluorescent labelling agents or, as detailed below, a photoactivatable cross-linking agent. in the dark, a strand with a photolabile group adjacent to th ... | 1987 | 2442728 |
| histological and immunological features of primary kaposi's sarcoma: evaluation before and after chemotherapy. | forty-one patients with primary kaposi's sarcoma (ks) have been evaluated clinically, histologically and immunologically at the time of diagnosis. there was no correlation between histological and immunological features. moreover, the disease did not appear to be related to particular hla phenotypes. the t4/t8 ratio was augmented. leu 7+ cells were also significantly increased. the last 15 patients who received chemotherapy were recently reevaluated after treatment and an increase in b lymphocyt ... | 1987 | 2442933 |
| screening of thyroid function in the hospitalized aged. | when a thyroid test was made on all the patients coming to a geriatric department of a hospital, markedly more new cases of hypothyroidism (17 as against 2), and also somewhat more new cases of hyperthyroidism (34 as against 21) were found than when the thyroid test was used only on clinical suspicion of thyroid dysfunction. the authors consider a thyroid test (t4?) worth taking systematically in all aged patients admitted to hospital. | 1987 | 2443092 |
| selective inhibition by methoxyamine of the apurinic/apyrimidinic endonuclease activity associated with pyrimidine dimer-dna glycosylases from micrococcus luteus and bacteriophage t4. | the uv endonucleases [endodeoxyribonuclease (pyrimidine dimer), ec 3.1.25.1] from micrococcus luteus and bacteriophage t4 possess two catalytic activities specific for the site of cyclobutane pyrimidine dimers in uv-irradiated dna: a dna glycosylase that cleaves the 5'-glycosyl bond of the dimerized pyrimidines and an apurinic/apyrimidinic (ap) endonuclease that thereupon incises the phosphodiester bond 3' to the resulting apyrimidinic site. we have explored the potential use of methoxyamine, a ... | 1987 | 2443160 |
| characterization of lymphocyte subpopulations in human cord blood using the immunogold staining technique. | lymphocyte subpopulations in human cord blood have been examined using monoclonal antibodies, visualized with immunogold. the proportions of t11, t4, t8, and b1 cells in cord blood are very similar to values in adult peripheral blood. some evidence of lymphocyte immaturity in cord blood is suggested by the presence of 12% calla-positive cells and the sum of t4 and t8 cells significantly exceeding the number of t11 cells; however, there were no tdt-positive cells. the presence of calla-positive l ... | 1987 | 2443385 |
| [acute antiarrhythmia treatment with amiodarone and blood levels of thyroid hormones]. | amiodarone has a good antiarrhythmic effect administered either acutely or chronically. since the antiarrhythmic effect of chronically administered amiodarone has been thought to be dependent on a depression of thyroid function, we studied the peripheral hormonal pattern of 10 euthyroid patients with ventricular arrhythmias who had been responsive to the acute intravenous administration of the drug (10 mg/kg). during the first 12 hours following the drug administration, reverse t3, free t3 and f ... | 1987 | 2443414 |
| active transport of iodothyronines into human cultured fibroblasts. | thyroid hormone uptake into human cultured fibroblasts was studied using 2-min incubations with labeled iodothyronines. the results indicate the presence of an active t4 uptake process with two saturable sites with apparent km values of 1.9 and 141 nm, respectively, and an active t3 uptake process with two saturable sites with km values of 29 and 650 nm. the uptake of both hormones was energy dependent, i.e. inhibited by kcn or by incubation of the cells in the absence of glucose. by analogy wit ... | 1987 | 2443527 |
| structure and transcription termination of a lysine trna gene from xenopus laevis. | termination of rna polymerase iii transcripts commonly occurs at clusters of t residues. a t4 tract located 72 base-pairs beyond a lysine trna gene from xenopus laevis serves as an efficient termination site for the trna(lys) precursors synthesized from this gene in homologous cell-free extracts. nucleotides following this t tract influence the extent of read-through transcription in vitro, but in a way that differs from xenopus 5 s rna termination. only approximately 50% of the transcripts init ... | 1987 | 2443712 |
| recognition of a purified mr-40,000 organ-specific immunogen of human lung cancer by class ii-restricted t-cells. | an mr-40,000 polypeptide (p40) was purified from a lung cancer cell line on the basis of its antigenicity with leukocytes from lung cancer patients in an in vitro immunological assay of leukocyte adherence inhibition. here, the cells and mechanism responsible for recognizing the purified p40 organ-specific cancer neoantigen (osn) were studied. buffy coat leukocytes from lung cancer patients showed a bell-shaped dose response with a peak response at 0.75 micrograms/assay. mononuclear cells showed ... | 1987 | 2443737 |
| an unsuccessful attempt to isolate human immunodeficiency virus (hiv) from patients with primary immunodeficiency diseases. | isolation of the human immunodeficiency virus (hiv) was performed in 5 seronegative patients with primary immunodeficiency diseases (idds) who had no or little ability to produce antibodies and received a large amount of intravenous gammaglobulin (ivg) for a long period. in this study cultured cells were considered to be infected if the following 2 criteria were met: (1) they produced reverse transcriptase (rt) activity in the supernatant fluid and (2) expressed viral antigens. under experimenta ... | 1987 | 2444016 |
| evidence that 2,3,7,8-tetrachlorodibenzo-p-dioxin and thyroid hormones act through different mechanisms in human keratinocytes. | it has been proposed [j. d. mckinney, j. fawkes, s. jordan, k. chae, s. oatley, r. e. coleman, and w. briner (1985). environ. health perspect. 61, 41-53] that 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) produces toxic responses through persistent occupancy of nuclear thyroxine (t4) receptors, and that maintenance of receptor occupancy by supraphysiologic concentrations of thyroid hormones mimics tcdd toxicity [l. h. hong, j. d. mckinney, and m. i. luster (1987). biochem. pharmacol., 36, 1361-1365 ... | 1987 | 2444020 |
| [formation of ion channels in the escherichia coli cytoplasmic membrane after exposure to bacteriophages t4 and lambda]. | the effects of phage t4 and lambda on the ion permeability of the e. coli cytoplasmic membrane were studied. it was shown that the phage-induced depolarization of the membrane is coupled with a simultaneous increase in a transmembrane ph gradient. hence, the total value of the proton-motive force remains unchanged at moderate multiplicity of infection. the rise in the ph gradient occurs due to an increase in the activity of the redox h+-pump of the e. coli membrane. analysis of the temperature d ... | 1987 | 2444271 |
| evaluation of t4 and t3 binding kinetics in the thyroxine binding globulin abnormality of australian aborigines. | low serum total t4 associated with subnormal concentrations of thyroxine binding globulin (tbg) has been reported in up to 40% of euthyroid australian aborigines. it has been suggested that these subjects show both diminished concentration of tbg and reduced tbg affinity for t4 (sarne et al., 1985). we have compared 12 euthyroid aborigines with low t4 (total t4 44 +/- 5 nmol/l) and aborigines with normal t4 (t4 99 +/- 9 nmol/l, n = 12) using measurements of free t4 and t3 by equilibrium dialysis ... | 1987 | 2444367 |
| anomalous binding characteristics of human thyroxine binding globulin due to a dilution-dependent separation artefact. | contrary to the accepted view, a recent study using sephadex column separation suggested that thyroxine binding globulin (tbg) binds t4 and t3 with similar affinity, but with a much larger capacity for t4 than t3. we have evaluated this finding by comparing this separation method with equilibrium dialysis, taking account of the effect of serum dilution with each method. estimates of free t4 fraction by equilibrium dialysis (with magnesium chloride precipitation) were valid over a wide range of s ... | 1987 | 2444368 |
| erythrocyte ouabain binding in patients receiving thyroxine. | thyroid hormone action at the cellular level was investigated in euthyroid women who were receiving replacement thyroxine, and whose plasma t4 levels were above the upper reference limit for healthy subjects. there is evidence that erythrocyte sodium pump sites are reduced in number in patients with hyperthyroidism. these sites were measured by the ouabain binding capacity. plasma t4, free t3 and tsh were also measured, the latter by a high sensitivity fluoroimmunoassay. three groups of women we ... | 1987 | 2444369 |
| ribavirin pharmacodynamics in high-risk patients for acquired immunodeficiency syndrome. | ribavirin was administered orally in escalating doses for 2 or 4 weeks to 15 symptom-free, human immunodeficiency virus seropositive homosexual men with generalized lymphadenopathy. reverse transcriptase activity was inhibited during therapy when steady-state plasma concentrations were greater than 6 mumol/l. these concentrations were achieved with 1200 or 2400 mg/day for 2 weeks or a loading dose of 2400 mg/day for 3 days followed by 600 mg/day for 4 weeks. drug accumulation occurred at all dos ... | 1987 | 2444379 |
| rapid androgenic stimulation of alpha 2u-globulin synthesis in the perfused rat liver. | hepatic synthesis of alpha 2u-globulin and its mrna in the male rat is dependent on androgen, glucocorticoid, t4, insulin, and gh. some of these hormones may act directly on the liver, while others may influence alpha 2u-globulin synthesis through indirect physiological changes. in the present study the specific role of androgen in the synthesis of alpha 2u-globulin was examined in an in vitro liver perfusion system. the addition of 5 alpha-dihydrotestosterone to the medium perfused through live ... | 1987 | 2444430 |
| independent regulatory influence of growth hormone on the hepatic synthesis of alpha 2u-globulin. | administration of gh through sc injections to hypophysectomized male rats induces the hepatic mrna for alpha 2u-globulin (a male-specific urinary protein) from an undetectable level to 43.4% of the normal male level. the same treatment administered to hypophysectomized-gonadectomized rats and androgen-insensitive tfm rats induces alpha 2u-globulin mrna to a level of only 5-10% of that in the normal male. however, none of these types of animals shows an appreciable response when gh is administere ... | 1987 | 2444431 |
| bacteriophage t4 anticodon nuclease, polynucleotide kinase and rna ligase reprocess the host lysine trna. | host trnas cleaved near the anticodon occur specifically in t4-infected escherichia coli prr strains which restrict polynucleotide kinase (pnk) or rna ligase (rli) phage mutants. the cleavage products are transient with wt but accumulate in pnk- or rli- infections, implicating the affected enzymes in repair of the damaged trnas. their roles in the pathway were elucidated by comparing the mutant infection intermediates with intact trna counterparts before or late in wt infection. thus, the t4-ind ... | 1987 | 2444436 |
| thyroid function and thyrotropin levels in rabbits immunized to produce antibodies against thyroid hormones. | various parameters of thyroid function were studied in 27 rabbits, out of which 10 were immunized to produce antibodies against triiodothyronine (t3), 9 against thyroxine (t4) and 8 were normals. estimations of t3, t4, free t4 (ft4) and thyrotropin (tsh) in blood, qualitative and quantitative analysis of iodoamino acids in serum, protein bound iodine-131 (pb131i), butanol extractable iodine-125 (be125i) and measurement of the disappearance rates of 125i-labelled t3 and t4 from plasma were done. ... | 1987 | 2444567 |
| neutralisation of hiv isolates by anti-idiotypic antibodies which mimic the t4 (cd4) epitope: a potential aids vaccine. | polyclonal anti-idiotypic antibodies were raised in mice against anti-leu3a, a mouse monoclonal anti-human t4 (cd4) antibody that blocks the in-vitro binding of human immunodeficiency virus (hiv) to the cd4 molecule. the anti-idiotypes recognized anti-leu3a but not okt4, an anti-human t4 antibody that does not inhibit hiv binding to cd4. the anti-idiotypes specifically reacted with the hiv envelope glycoprotein in solid-phase immunoassays. more importantly, the anti-idiotypes neutralised three d ... | 1987 | 2444842 |
| [lymphocytes: recent findings]. | in the last ten years, considerable progress has been made in our understanding of the physiology of the lymphoid system. these include advances in knowledge of the biochemical nature of the receptors used by b and t lymphocytes in antigen recognition, the origin of the diversity of specificity exhibited by these receptors, and the crucial role played by growth factors in the cellular proliferation elicited during immune response. moreover, the biological significance of the existence of two sub ... | 1987 | 2445021 |
| evaluation of thyroid function in children with undiagnosed short stature in north india. | fifty-five children with short stature were investigated for the aetiology of short stature with special reference to hypothyroidism. clinical and laboratory parameters including anthropometry were determined to exclude any chronic systemic disorders. thyroid function tests such as thyroxine (t4) and thyroid stimulating hormone (tsh) estimation by radioimmunoassay, radioactive iodine uptake and thyroid scan, using 131i and perchlorate discharge test, were performed. in addition, growth hormone w ... | 1987 | 2445271 |
| immunologic dysregulation in a patient with familial hemophagocytic lymphohistiocytosis. | a 6-year-old jewish iranian girl with familial hemophagocytic lymphohistiocytosis (fhlh) is described. the course of the disease fluctuated with partial initial response to antibiotics, steroids, and supportive treatment. subsequent cytotoxic treatment, including vp-16, velban (vinblastine sulfate), and methotrexate (mtx) controlled the disease for a few months but the child died with a clinical picture of meningocephalitis 1.5 years later. benign-looking lymphohistiocytic infiltrates with varyi ... | 1987 | 2445462 |
| somatosensory evoked potentials and auditory brain-stem responses in congenital hypothyroidism. ii. a cross-sectional study in childhood. correlations with hormonal levels and developmental quotients. | we report the results of somatosensory evoked potentials (seps) and auditory brain-stem responses (abrs) done in 48 congenital hypothyroid (ch) treated children, early detected and aged 18 months, 3 and 5-9 years of age. we report also the results of sep and abr done in 9 3-year-old ch children, before and after a 1 month therapy interruption to reassess the thyroid status. the more frequent abnormalities were increased wave i latencies for 18 month and 3-year-old ch children. in some children, ... | 1987 | 2445544 |
| thyroid angiogenesis: endotheliotropic chemoattractant activity from rat thyroid cells in culture. | thyroid enlargement in response to chronic hypersecretion of tsh reflects the coordinated growth of both parenchyma and stroma. because wollman et al. observed in propylthiouracil-fed rats that enlargement and remodeling of thyroid capillaries were strictly localized around follicles, they hypothesized that growth of perifollicular blood vessels is stimulated by angiogenic factors secreted by neighboring follicular epithelial cells. in support of this hypothesis, we report that media conditioned ... | 1987 | 2445558 |
| identification of the sequence required for expression of the 2h4 epitope on the human leukocyte common antigens. | the anti-2h4 antibody, which subdivides the t4+ population of human t lymphocytes into t4+, 2h4+ suppressor-inducer cells and t4+, 2h4- helper cells, recognizes an epitope on a subset of the human leukocyte common antigens (lcas). lcas are a family of cell surface glycoproteins generated from a single gene by the differential usage of three exons near the nh2-terminus. using cdna clones corresponding to four of the different forms of lca molecules, extracellular domains of the lca molecules were ... | 1987 | 2445891 |
| measurements of serum-free thyroid hormone concentrations by ultrafiltration--a comparison with equilibrium dialysis and mathematical calculation. | an ultrafiltration method (uf) for measuring free thyroxine (ft4) and free triiodothyronine (ft3) using the diaflow ym membrane (centricon-10) is described. the results are compared with those by equilibrium dialysis (ed) and also by mathematical calculations derived from t4, t3, and binding protein concentrations. the precision with the uf method was excellent. the normal ranges of ft4 and ft3 by the three methods are all comparable. there was a high degree of correlation of ft4 or ft3 results ... | 1987 | 3275093 |
| detection of t8 (suppressor/cytotoxic) lymphocytes in human ovarian follicular fluid. | clear ovarian follicular fluid and the follicular flush (bloody) of aspirated follicles from 18 women undergoing oocyte retrieval for in vitro fertilization were tested for the presence of t-lymphocyte subpopulations with the use of a panel of monoclonal antibodies in indirect immunofluorescence assay. a sample of peripheral venous blood from each patient was run as a control. there was no difference in the percentage of cells positive for the various markers between bloody follicular fluid (i.e ... | 1987 | 3491766 |
| characterization of immunologic function in homosexual men with persistent, generalized lymphadenopathy and acquired immune deficiency syndrome. | a syndrome of persistent, generalized lymphadenopathy (pgl), related to the acquired immune deficiency syndrome (aids), has been described in homosexual men. to further characterize and correlate the immunologic status of patients with pgl with those in aids, we studied spontaneous and pokeweed mitogen (pwm)-induced igg synthesis by b-cells, t-cell subsets in peripheral blood (pb), natural cytotoxicity (nc), and interleukins (il)-1 and il-2 production in 39 homosexual patients (21 pgl; 13 aids; ... | 1987 | 3492262 |
| interleukin 2 production and responsiveness in individuals with acquired immunodeficiency syndrome and the generalized lymphadenopathy syndrome. | proliferative responsiveness to, and production of, interleukin 2 (il-2) was determined in 9 homosexually active men with the acquired immunodeficiency syndrome (aids) and in 28 homosexually active men with the persistent generalized lymphadenopathy syndrome (pgl). all were seropositive for antibody to human t-lymphotrophic virus type iii/lymphadenopathy-associated virus (htlv-iii/lav). purified t lymphocytes from individuals with aids and pgl had a significantly decreased (p less than 0.01) pro ... | 1987 | 3492294 |
| monoclonal antibodies can enhance the biological activity of thyrotropin. | in this work we demonstrate that monoclonal antibodies (mabs) to tsh can enhance the biological actions of tsh in vivo. hypopituitary snell dwarf mice were injected with tsh (25, 50, or 100 mu/day) alone or complexed with mab-gc73 once per day for 5 days; control animals received pbs. radioactive sulfate (35so4(2-)) was also injected on the fifth day and animals were killed 20 h later. thyroids were removed for histology, blood taken for t4 estimations by ria, and 35so4(2-) uptake into costal ca ... | 1987 | 3492367 |
| suppression of peripheral blood natural killer cell activity by excess thyroid hormone. | natural killer (nk) cells were assessed in patients with hyperthyroxinemia due to graves' disease or treatment with thyroxine (t4). cytolytic activity was measured with 51cr-labeled k562 tumor cells and nk enumeration was by flow cytometry using nkh-1 monoclonal antibody to identify the relevant surface marker. activity was uniformly decreased in association with hyperthyroxinemia, regardless of the underlying pathology; however, there was no reduction in the number of nkh-1+ cells. nk activity ... | 1987 | 3492513 |
| effect of t4 count and cofactors on the incidence of aids in homosexual men infected with human immunodeficiency virus. | we prospectively evaluated potential markers and cofactors for the acquired immunodeficiency syndrome (aids) in 86 homosexual men who were seropositive for human immunodeficiency virus antibodies. during three years of follow-up, 19 men developed aids. risk of aids was clearly predicted by the total number of circulating okt4-positive lymphocytes (t4 count) at enrollment, while the corresponding t8 count was unrelated to subsequent aids development. subjects in manhattan had a higher risk of kap ... | 1987 | 3491911 |
| peripheral blood t-cell subpopulations in the very low birth weight (less than 1,500-g) infant. | the development of monoclonal antibodies to cell-surface antigens has provided method for characterizing distinct subpopulations of t-cells. in the present study we have quantified peripheral blood t-cell subpopulations in premature infants born weighing less than 1,500 g (1123 +/- 223 g) and ranging in gestational age from 25 to 32 weeks. the relative proportion of t4 cells in the very low birth weight (vlbw) infants was markedly higher at 1 week and 1 month of age (mean +/- sem; 67.5 +/- 4.1 a ... | 1987 | 3492138 |
| evaluation of circulating malignant cells provides prognostic information in cutaneous t cell lymphoma. | peripheral blood lymphocyte morphology was evaluated prospectively by light microscopy of blood smears and e rosette preparations in 160 patients with cutaneous t cell lymphoma (ctcl). blood involvement was related to the type of cutaneous t-stage, being present in 90% of patients with erythroderma (t4), 27% of those with cutaneous tumors (t3), 9% of those with generalized (t2), and 0% of those with limited skin plaques (t1). untreated patients with blood involvement (38 of 105) had a higher fre ... | 1987 | 3493044 |
| lymphocyte subpopulations, activation phenotypes, and spontaneous proliferation in tuberculous pleural effusions. | the state of lymphocyte activation in tuberculous pleural effusions was studied. the proportion of cells at and beyond the g1 phase of the cell cycle displaying interleukin-2 receptor, transferrin receptor or gp 40/80 glycoprotein in avidin-biotin-peroxidase complex (abc) staining was 6 +/- 2 percent, 8 +/- 3 percent, and 14 +/- 4 percent of all pleural fluid mononuclear cells, respectively. these findings imply that only a fraction of pleural fluid lymphocytes is activated in tuberculosis. the ... | 1987 | 3493118 |
| an acute granular lymphoid leukemia with unusual cytochemistry and immunologic phenotype. | a 15-year-old mexican boy presented with an l2 type acute lymphoid leukemia (all). occasional granulated and hand mirror cells were observed, and enzyme histochemistry showed the blasts to be positive for acid phosphatase, beta glucuronidase, and alpha napthyl butyrate esterase. immunologic studies showed the cells to stain for terminal deoxynucleotidyl transferase and common all antigen and to react with b4 (90 per cent), b1 (60 per cent), and b2 (37 per cent) monoclonal antibodies. t-cell-asso ... | 1987 | 3493198 |
| an antiviral t-cell clone defines a functional supertypic specificity shared by different hla-dr molecules from dr2-short, drw11, and drw13 haplotypes. | an influenza virus-specific hla class ii-restricted human t4+ clone (ij) allows us to define a new functional supertypic hla class ii specificity shared by three different haplotypes. influenza a virus-infected antigen-presenting cells of these three haplotypes, hla-dr2 short, drw11, and drw13, are able to stimulate ij cells. the same precise viral specificity is seen in all three cases. proliferation inhibition experiments using hla-specific monoclonal antibodies demonstrate that hla-dr product ... | 1987 | 3493215 |
| skin test responsiveness to four new tuberculins in patients with chronic obstructive airways disease receiving short term high doses of, or long term maintenance treatment with, prednisolone: clinical appearances and histometric studies. | the response to skin testing with tuberculins extracted from various species of mycobacteria was studied in 49 patients from dundee with chronic obstructive airways disease. seventeen had never been treated with steroids (group 1), 17 were receiving short term high doses of prednisolone (group 2) and did not have impaired synacthen tests; 15 were receiving long term maintenance treatment and did have impaired synacthen tests (group 3). erythematous and indurated reactions were seen in a few pati ... | 1987 | 3493262 |
| serum free thyroxine and thyroxine-binding globulin concentrations in early phase of subacute thyroiditis. | serum total thyroxine (t4), total triiodothyronine (t3), t4-binding globulin (tbg), free t4(ft4) and free t3(ft3) concentrations and the t3-uptake(t3-u) value were estimated in 11 patients with subacute thyroiditis, and compared with the same parameters in 11 patients with graves' disease, whose serum t4 concentrations were similar to the former group. seven patients with subacute thyroiditis, who were treated with dicrofenac sodium alone, were investigated as to the sequential changes in serum ... | 1987 | 3131131 |
| cloning and purification of a unique lysozyme produced by bacillus phage phi 29. | a dna fragment of the bacteriophage phi 29 chromosome, encoding the entire sequence of phi 29 gene 15, has been cloned into the escherichia coli expression vector pplc245 under the control of the phage lambda major leftward promoter, pl. upon heat induction, a protein with an apparent molecular mass of 26 kda was overproduced. the molecular mass of this protein corresponds to the 28 kda predicted for the product of gene 15 from its nucleotide sequence. the overproduced protein has been purified ... | 1987 | 3469652 |
| oligonucleotides with rapid turnover of the phosphate groups occur endogenously in eukaryotic cells. | endogenous oligonucleotides were found in trichloroacetic acid extracts of hamster lung fibroblasts and tetrahymena cells. peaks of radioactivity that eluted with retention times similar to oligonucleotide markers (5- to 50-mer) were found by hplc in cells labeled briefly with 32pi. only minute amounts of uv-absorbing material were detected, consistent with a rapid turnover of phosphate groups. the 32p-labeled material also migrated as oligonucleotides on 20% polyacrylamide gels; it was not hydr ... | 1987 | 3470767 |
| prevalence, clinical significance, and strain specificity of neutralizing antibody to the human immunodeficiency virus. | a semiautomated microtiter assay has been developed to quantitate neutralizing antibody to the human immunodeficiency virus. this assay has been found to be highly specific. forty-four sera that were negative by enzyme-linked immunosorbent assay (elisa) were tested under code: 42 were negative (less than 1:2), and 2 had titers of 1:2. by contrast, of 178 sera positive by western blot, 92.7% had detectable neutralizing antibody, and 12.5% had titers greater than or equal to 1:128. neutralizing an ... | 1987 | 3474300 |
| [the evaluation of determination of mb, t3, t4 by radioimmunoassay in burned patients]. | 1987 | 3151629 | |
| isolation of pure and functionally active t4 and t8 cells by positive selection with antibody-coated monosized magnetic microspheres. | 1987 | 3152700 | |
| development of natural killer cells in human thymocyte culture: regulation by accessory cells. | in vitro culture of human thymocytes resulted in the development of cells with natural killer (nk) activity and the acquisition of a pan-nk antigen (nkh1) by a large number of thymocytes. the ability to kill the nk-sensitive target, k562, was restricted to thymocytes expressing the nkh1 antigen. all nkh1+ thymocytes displayed a mature t cell phenotype, t3+t11+t8+t4-. both the acquisition of nk activity and the development of cells with the nkh1+ phenotype could be suppressed by culturing thymocy ... | 1987 | 3495443 |
| some aspects of "deep lung" cellular immunity in chronic bronchitis before and after therapy with tiopronin. | fifteen patients suffering from obstructive chronic bronchopneumonia in a clinical steady-state phase received 0.6 g of tiopronin once a day during 10 days per os. the lymphocyte subsets (okt3, okt4 and okt8) have been identified in peripheral blood and in bronchoalveolar lavage liquid (bal) before and after treatment and phagocytosis has been evaluated in pulmonary alveolar macrophages (pam) (phagocytosis index, phagocytosis percentage, superoxide-ion production). a highly significant improveme ... | 1987 | 3495498 |
| chronic vaginal candidiasis in women with human immunodeficiency virus infection. | twenty-four percent (7/29) of human immunodeficiency virus-infected women seen at walter reed army medical center between 1983 and 1986 presented with a history of chronic refractory vaginal candidiasis. in addition to vaginal candidiasis, all patients were found to have oral thrush on physical examination, severe t-helper cell depletion (mean t-helper cell count of 90), and markedly decreased t4/t8 ratios (mean, 0.3); six of seven women were anergic by delayed-type hypersensitivity skin testing ... | 1987 | 3495672 |
| effects of enterally fed epidermal growth factor on the small and large intestine of the suckling rat. | epidermal growth factor (egf) has been shown to be present in the milk of several species, including the rat, and to have gastrointestinal effects when given parenterally or orally in pharmacologic doses. we investigated the effect of enteral egf in physiologic doses on the small intestine and colon of suckling rats. serum thyroxine (t4) levels were also measured. rats were gavage-fed by hand with an artificial formula with or without added egf every 3 h from 11 to 14 days of age. intake was adj ... | 1987 | 3495822 |
| hiv antibody status and immunological abnormalities in polish haemophiliacs. | sera of 520 multitransfused haemophiliacs were examined for antibody to hiv; 447 patients had haemophilia a and 73 had haemophilia b. in 382 patients with haemophilia a and in 62 with haemophilia b solely polish-made blood products were used for replacement therapy. the remaining haemophiliacs had also received imported clotting factor concentrates prior to the investigation. only 8 patients (haemophilia a - 7, haemophilia b - 1) developed anti-hiv and all of them had been exposed to commercial ... | 1987 | 3495899 |
| natural history of human immunodeficiency virus infections in hemophiliacs: effects of t-cell subsets, platelet counts, and age. | serial t-cell subsets and platelet counts were determined in a cohort of 84 hemophiliacs in whom time of seroconversion for human immunodeficiency virus (hiv) antibody could be ascertained. an abrupt decrease in the number of t-helper (t4) cells was seen in 9 patients 12 to 24 months before the acquired immunodeficiency syndrome (aids) was diagnosed (p = 0.0007 compared with those who did not develop aids). thrombocytopenia also was associated with an increased risk for aids (p = 0.02), as was o ... | 1987 | 3496028 |
| in vitro leukemic cell differentiation in metal-depleted media. | lymphocytes of children with t cell and pre-b cell acute lymphocytic leukemia (all) were cultured in media with different concentrations of fe, cu and zn to detect a possible effect of these ions on leukemic cells. thymidine uptake was elevated in non-mitogen stimulated all cells and increased after mitogen stimulation, whereas intracellular ferritin, fe and cu, elevated before culture, decreased thereafter; pre-b all cells, positive only for cytoplasmic mu chains, became positive for surface im ... | 1987 | 3496041 |
| exogenous triiodothyronine activates bone remodeling. | the effect of exogenous triiodothyronine (t3) as an activator of bone remodeling was investigated by measuring biochemical marker levels. fourteen people received 100-160 micrograms of t3 for 1 week and were followed for a total of 10 weeks. serum t3 increased markedly during the first week, whereas serum tsh and serum thyroxine (t4) decreased. bone resorption was stimulated during the first week as evaluated from serum calcium and renal excretion of calcium, phosphate, and hydroxyproline. serum ... | 1987 | 3496108 |