Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [magnetic resonance imaging: early detection of involvement of the central nervous system in acquired immunodeficiency syndrome]. | central nervous system (cns) involvement, whether primary by the human immunodeficiency virus--hiv--itself, or secondary (toxoplasmosis or lymphoma) is remarkably frequent in aids, in 40 to 70% of cases, depending upon the author. in order to study the natural history of this illness, a cohort of 25 asymptomatic seropositive patients have been established. every 6 months these patients undergo biological and clinical examinations, as well as magnetic resonance brain scans. after two examinations ... | 1988 | 3142638 |
| 3,5,3'-triiodothyroacetic acid minimizes the pituitary thyrotrophin secretion in patients on levo-thyroxine therapy after ablative therapy for differentiated thyroid carcinoma. | the aim of this study was to examine the influence of 3,5,3'-triiodothyroacetic acid (triac) on serum thyrotrophin (tsh) concentration in 25 patients on levo-thyroxine (l-t4) therapy after ablative therapy for differentiated thyroid carcinoma. the daily l-t4 intake amounted to 2.6 +/- 0.7 micrograms/kg body weight (+/- sd). serum tsh concentration was determined by means of a sensitive radioimmunometric method (lower detection limit 0.03 mu/l). the median (means) basal tsh concentration under l- ... | 1988 | 3142712 |
| normal cellular uptake of thyroxine from serum of patients with familial dysalbuminemic hyperthyroxinemia or elevated thyroxine-binding globulin. | to determine whether thyroid hormone-binding proteins in serum, particularly albumin, facilitate the transfer of t4 into human tissues, we studied cellular t4 uptake (ct4) by human liver (hep g2) cells from medium containing serum from subjects with familial dysalbuminemic hyperthyroxinemia (fdh) and acquired and familial t4-binding globulin (tbg) excess and patients with normal t4-binding to albumin and normal tbg concentrations. serum from nine subjects with fdh whose mean serum total t4 (tt4) ... | 1988 | 3142912 |
| thyroid functions in patients with various chronic liver diseases. | in order to clarify an alteration in thyroid functions in patients with chronic liver diseases, serum total and free thyroxine (t4, ft4), total and free triiodothyronine (t3, ft3), total reverse t3 (rt3), thyrotropin (tsh), thyroxine-binding globulin (tbg) concentrations, and t3 uptake (t3u) were measured by radioimmunoassays in 53 patients with chronic hepatitis (ch), 24 patients with compensated liver cirrhosis (lc), 17 patients with hepatocellular carcinoma associated with lc (hcc), and 40 no ... | 1988 | 3143545 |
| clinical utility and cost-effectiveness of sensitive thyrotropin assays in ambulatory and hospitalized patients. | in either an ambulatory or a hospitalized patient setting, a normal serum sensitive thyrotropin (tsh) value is strongly suggestive of euthyroidism if the patient has intact hypothalamic-pituitary function and is not receiving drugs known to suppress pituitary tsh secretion. in stable ambulatory patients, an abnormal sensitive tsh value is strongly suggestive of clinical or subclinical thyroid hormone excess or deficiency, which can be confirmed by a free thyroxine (t4) index (ft4i) and evaluatio ... | 1988 | 3143866 |
| long-term effects of thyrotropin-releasing hormone and histidyl-proline diketopiperazine on the maturation of homeothermia and mitochondrial enzyme activities in neonatal rats. | to elucidate the mechanism by which trh and its metabolite, histidyl-proline diketopiperazine (cyclo(his-pro], act on the maturation of homoiothermy, the chronic effects of intrathecal administration of the peptides on body temperature, serum thyroid hormone levels, and mitochondrial energy-producing enzyme activities were examined in neonatal rats. the two peptides or an equimolar mixture of both were injected intrathecally at a dose of 3, 6 and 9 nmol for 7 consecutive days during the 1st, 2nd ... | 1988 | 3144109 |
| effects of aging on the pituitary-thyroid axis in the dog. | the effects of advancing age on basal serum concentrations of thyroxine (t4) and thyrotropin (tsh), on t4 responses to tsh, and on tsh responses to thyrotropin-releasing hormone (trh) were studied in beagle dogs. a total of 27 female dogs belonging to four age-groups were used: prepubertal (11.4 +/- 0.2 (sd) weeks), adult (2.1 +/- 0.3 years), middle-aged (6.5 +/- 0.2 years), and old (12.4 +/- 0.3 years). there was no significant difference between serum t4 concentrations of prepubertal (4.3 +/- ... | 1988 | 3144459 |
| multiple roles of dna ligase at the replication fork. | the loss of superhelical turns from a covalently closed duplex dna exposed to bacteriophage t4 dna ligase in the presence of amp and mg2+ has recently been found to be gradual and not sudden (montecucco, a. and ciarrocchi, g. (1988) nucleic acids res. 16, 7369-7381). in this paper, we show that the amp-dependent dna relaxation catalyzed by human and e. coli dna ligases also takes place according to a step-wise mechanism. dna relaxation is inhibited by pyrophosphate, by atp (or nad in the case of ... | 1988 | 3145019 |
| toxic potential of non-ortho and mono-ortho coplanar pcbs in commercial pcb preparations: "2,3,7,8-t4 cdd toxicity equivalence factors approach. | 1988 | 3145047 | |
| a unitary basis for different hairy-wing mutations of drosophila melanogaster. | hairy-wing (hw) mutations are caused by modifications of the achaete-scute complex (as-c) which promote development of extra sensory organs on the cuticle of drosophila melanogaster. we show that the extreme hw49c allele contains an inversion with a breakpoint within the as-c, while the weak hw685 allele is associated with a terminal deletion of the x chromosome which removes the achaete region of the as-c. in both cases, foreign dna in contact with the breakpoints presumably enhances expression ... | 1988 | 3145198 |
| [behavior of the basal and stimulated serum level of thyroid stimulating hormone and determination of thyroxine, thyroxine- binding capacity and free thyroxine index in females with chronic uremia]. | in 11 female patients with chronic uraemia at the age of 20 to 47 years (average age 33.1 years) the basal and the thyrotropin releasing hormone-(trh-) stimulated thyroid gland stimulating hormone-(sh-) secretion were investigated, in addition to this the parameters of the thyroid gland total thyroxin (t4), thyroxin binding capacity (tbc) as well as the free thyroxin-index (ft4-i). in 2 women the investigations were repeated after kidney transplantation. the determination of tsh and t4 was carri ... | 1988 | 3145649 |
| immunological studies in focal epilepsy. | serum immunoglobulins, t-lymphocyte subsets and hla investigations were carried out in 24 patients with focal, mainly temporal lobe, epilepsy and in 30 of their first degree relatives. the mean serum level of iga was significantly decreased in the epileptic probands compared with controls. in the relatives, there was a significant decrease in mean igm levels. the epileptic group had significantly fewer circulating t4 "helper" lymphocytes (absolute and percent) and an increased percentage of t8 " ... | 1988 | 3146208 |
| effects of cold exposure or trh on the serum tsh levels in the iron-deficient rat. | normal and iron-deficient rats were exposed to cold at 4 degrees c for 1 hr or 5 hrs and the serum tsh, t3 and t4 levels were compared with those in rats kept at room temperature (20 degrees c). there was a rise in serum tsh, t3 and t4 levels in response to 1 hr and 5 hrs of cold exposure in normal, but not in iron-deficient rats. although pituitary tsh contents were lower in iron-deficient rats, the increases in serum levels of tsh following administration of trh were similar in both normal and ... | 1988 | 3146537 |
| the pituitary-thyroid axis in acromegaly. | the pituitary-thyroid axis of 12 acromegalic patients was evaluated by measurement of the serum concentrations (total and free) of thyroxine (t4), triiodothyronine (t3) and reverse t3 (rt3) and thyrotropin (tsh), growth hormone (gh) and prolactin (prl) before and after iv stimulation with thyrotropin releasing hormone (trh). using an ultrasensitive method of tsh measurement (irma) basal serum tsh levels of the patients (0.76, 0.07-1.90 miu/l) were found slightly, but significantly (p less than 0 ... | 1988 | 3146541 |
| thyroid function in patients maintained on lithium. | to determine the clinical significance of thyroid function abnormalities in patients maintained on lithium, the authors evaluated the relationships of thyroid function tests to clinical response to lithium and side effects from lithium in 20 outpatients meeting dsm-iii criteria for major affective disorder. no significant relationships were found between baseline thyroid function tests and clinical response. thyroxine (t4) and triiodothyronine uptake ratio (t3ur) within the normal range were fou ... | 1988 | 3146759 |
| [a case of neurofibromatosis with spinal dural ectasia and vertebral body scalloping: a case report]. | a 41-year-old man noticed colorless cutaneous nodules all over his body at the age of 24. recently the nodules increased markedly in number and several large nodules were removed at the dermatological clinic of our hospital. the histological diagnosis of the nodules was neurofibroma. he was then referred to our neurosurgical clinic for neurological examinations to rule out involvement of the central nervous system. he showed no abnormality in neurological examination except for hyper-reflexia of ... | 1988 | 3147405 |
| elevation of 2',5'-oligoadenylate synthetase activity and hla-i associated beta 2-microglobulin in response to recombinant interferon-gamma administration in chronic hbeag-positive hepatitis. | we have analyzed the immunomodulatory effect of 5 and 2 mu of recombinant interferon-gamma (rifn-gamma) administered to 8 carriers of hbsag with histologically proven chronic active liver disease. after the rifn-gamma administration, t8 lymphocyte subsets showed a significant decrease (basal vs. 4 weeks, p less than 0.05) and t4/t8 ratios were higher than the basal values in 6/8 patients. serum levels of the hla class i-associated beta 2-microglobulin increased significantly in all patients with ... | 1988 | 3148006 |
| [dynamic thyroid function after the surgical treatment of patients with diffuse toxic goiter]. | the time course of thyroid function and hypophyseal thyrotropic activity were investigated in 182 patients with diffuse toxic goiter of severe and moderately severe forms of thyrotoxicosis before operation and in short- and long-term periods after operation using radioimmunoassay in a hospital and outpatient clinic. the determination of blood t3, t4 and tsh at the height of clinical manifestations of thyrotoxicosis and before operation was shown to reflect the gravity of disease and could serve ... | 1988 | 3148927 |
| anorexia nervosa with elevated serum tsh. | low or normal serum tsh concentration is common during fasting and in patients with anorexia nervosa. we report here four patients with anorexia nervosa who had elevated serum tsh, low t3 and low free t4 levels when the initial diagnosis was made. also, an appearance of tsh peak in response to trh was delayed, but t3 responsiveness to trh was normal. all patients were clinically euthyroid with negative serum thyroid autoantibodies and without goiter. following weight gain, basal levels of serum ... | 1988 | 3148959 |
| the effects of trh on plasma thyroid hormone levels of rainbow trout (salmo gairdneri) and arctic charr (salvelinus alpinus). | plasma levels of l-thyroxine (t4) and 3,5,3'-triiodo-l-thyronine (t3) were measured in 2- to 4-year-old rainbow trout (salmo gairdneri) and arctic charr (salvelinus alpinus) intraperitoneally injected with 0.7% nacl alone (controls) or 0.7% nacl containing synthetic thyrotropin-releasing hormone (trh). blood was obtained from terminally sampled fish or from serially sampled fish cannulated in the dorsal aorta. in trout, trh (1 microgram/g body wt) significantly raised plasma t4 to maximal values ... | 1988 | 3149247 |
| thyrotropin-releasing hormone enhances event-related brain potentials and growth hormone release in man. | changes in contingent negative variation (cnv), a brain-evoked potential related in arousal, as well as in serum triiodothyronine (t3), thyroxine (t4), thyroid-stimulating hormone (tsh), prolactin (prl), cortisol and growth hormone (gh) levels were recorded in 12 male volunteers receiving either thyrotropin-releasing hormone (trh) or saline infusion. only during trh administration an increase in cnv (p less than 0.02) and, 30 min later, in gh (p less than 0.05) occurred; thyroid hormones and prl ... | 1988 | 3149400 |
| acceleration of the internalization of ligand-bound membrane dna by thyroxine treatment in macrophages. | the localization of membrane dna and the binding and internalization of dnase-colloidal gold complex were examined in mouse peritoneal macrophages in presence and absence of thyroxine (t4). preexposure to the hormone for 5 to 10 min caused no change relative to the control but preexposures for 30, 60, and 90 min accounted for an increase in the ligand binding capacity, aggregation of the dnase-gold particles on the cell surface, and appearance thereof in coated pits, coated vesicles, smooth vesi ... | 1988 | 3149441 |
| a 3,3',5-triiodothyronine autoantibody (igg, lambda) in a case of hashimoto's thyroiditis. | a 19-year-old japanese woman with hashimoto's thyroiditis appeared to be hypothyroid by clinical and laboratory criteria. however, the serum levels of total t3 and free t3 as measured by solid-phase radioimmunoassay were not compatible with the clinical features and other laboratory data, therefore the presence of serum t3 autoantibodies was suspected. gel filtration analysis (sephacryl s-300) of acid-treated serum trace-labeled with 125i-t3 revealed binding to high and intermediate molecular fr ... | 1988 | 3149557 |
| effect of thymic extract 'thymostimulin' on the incidence of infections and myelotoxicity during adjuvant chemotherapy for breast cancer. | a clinical trial was made to evaluate the effectiveness of thymostimulin (tst), a bovine thymic extract, in preventing infections in breast cancer patients treated with polychemotherapy. fifty-one women treated with adjuvant cmf were randomly divided into two groups: 25 patients received tst plus chemotherapy and 26 patients were subjected to chemotherapy only. periodic clinical and laboratory checks were performed for at least three months to monitor the occurrence of infections and variations ... | 1988 | 3150630 |
| the role of a highly sensitive amplified enzyme immunoassay for thyrotrophin in the evaluation of thyrotroph function in hypopituitary patients. | using a highly sensitive amplified enzyme-linked immunoassay (aeia) for thyrotrophin (tsh), we have assessed the ability of a single basal measurement of tsh to predict the subsequent response of tsh to trh in a group of 11 patients with known pituitary pathology and some evidence of hypopituitarism. all patients were clinically euthyroid. basal levels of aeia-tsh ranged from less than 0.2 mu/l ('undetectable') to 0.9 mu/l; within this range there was no correlation with the subsequent tsh level ... | 1988 | 3150823 |
| effect of simultaneously administered iopanoic acid and thyrotropin releasing hormone (trh) on thyroid hormone metabolism in euthyroid subjects. | the cause of serum t4 elevation after iopanoic acid administration was searched in euthyroid subjects. 3 gr iopanoic acid was given per os to 9 healthy volunteers (group i), in other 5 volunteers a trh test was performed prior to iopanoic acid administration (group ii). in group i tsh, t4, ft4i and rt3 levels increased, in group ii t3 and ft3i decreased, but no tsh, t4, ft4i increase was seen, obviously, because of the exhaustion of pituitary tsh content by trh. on the ground of the findings t4 ... | 1988 | 3150834 |
| humoral and cellular parameters of the immune system of cebus apella monkeys. cross reactivity between monkey and human immunoglobulins. | the humoral and cellular immunological parameters of the new world non-human primate cebus apella were analysed. the study included: serum protein immunoelectrophoretic analysis; cross reactivity between monkey and human immunoglobulins by immunoprecipitation, elisa and indirect immunofluorescence tests; immunoglobulin quantitation by radial immunodiffusion; and assays with peripheral blood lymphocytes involving tests for e and eac rosettes and detection of surface markers (surface immunoglobuli ... | 1988 | 3150887 |
| thyrotrophin-releasing hormone induces growth hormone secretion in adult hypothyroid fowl. | while thyrotrophin-releasing hormone (trh) stimulated growth hormone (gh) secretion in adult anesthetized cockerels, the gh response was blocked in anesthetized birds pretreated with thyroxine (t4) or triiodothyronine (t3). moreover, whereas gh secretion in conscious adult birds was poorly responsive to trh stimulation, conscious birds made hypothyroid by goitrogen pretreatment (with propylthiouracil, methimazole, or thiourea) were responsive to trh challenge. basal circulating gh concentrations ... | 1988 | 3130285 |
| cultured human bone marrow-derived mast cells, their similarities to cultured murine e-mast cells. | homogeneous populations of human mast cells were differentiated and grown by culturing bone marrow cells in the presence of conditioned medium derived from lectin-stimulated human peripheral blood mononuclear cells. the cells obtained were similar in ultrastructure, proteoglycan type and lipid products generated upon calcium ionophore a23187, and immunological activation to the murine e-mast cells (e-mc) differentiated in culture containing il-3. fluorescence analysis revealed that the human e-m ... | 1988 | 3130309 |
| [pericarditis and acquired immunodeficiency syndrome]. | in order to evaluate the frequency and clinical features of pericarditis caused by the hiv virus, 17 patients (mean age 28 years) presenting with pericarditis were investigated at the university clinics of mont-amba (zaïre), between january, 1985 and december, 1986. the clinical diagnosis of aids had been made on the basis of the who criteria. an elisa test, a tuberculin test and a t4-lymphocyte count were performed in all patients. cardiovascular explorations were limited to electrocardiography ... | 1988 | 3130820 |
| the normal scalp topography of the middle latency auditory evoked potential pa component following monaural click stimulation. | the scalp topography of the middle latency auditory evoked potential (mlaep) pa component following left and right ear click stimulation was investigated in 15 normal hearing and neurologically intact right-handed subjects. an unbiased reference was employed. the pa component showed a broad voltage field that was recorded maximally at the cz and fz leads regardless of which ear was stimulated. a broad negative voltage field that occurred coincident in time with the pa component was recorded post ... | 1988 | 3274960 |
| amiodarone inhibits t4 and t3 secretion but does not affect t4 deiodination to t3 in perfused dog thyroid lobes. | amiodarone interferes with thyroid hormone homeostasis in several ways. in the present study we assessed if amiodarone has direct effects on thyroid secretion of t4 and t3 and on intrathyroid t4 deiodination to t3 as studied in perfused dog thyroid lobes. 10(-4) mol/l amiodarone inhibited the tsh stimulated t4 secretion to 73.0 +/- 8.3 per cent of control (mean +/- se, n = 4, p less than 0.05) and t3 secretion to 68.3 +/- 6.2 per cent of control (p less than 0.05). amiodarone did not alter intra ... | 1988 | 2484889 |
| reverse t3 and modulators of the calcium messenger system rapidly decrease t4-5'-deiodinase ii activity in cultured mouse neuroblastoma cells. | neural t3 neogenesis is modulated by the enzyme t4-5'-deiodinase type ii (t4-5'-dii). hypothyroidism increases the activity of rat pituitary and cerebral cortex enzyme activity. mouse neuroblastoma cells (nb41a3) incubated in thyroid hormone deficient medium also show a significant increase in t4-5'-dii activity. this response is rapidly (less than 30 minutes) reversed by reverse t3 (rt3) suggesting a mechanism independent of nuclear t3 receptor binding or new protein synthesis. this report deta ... | 1988 | 2484895 |
| lymphocyte subpopulations and lymphokine production in children with constitutional aplastic anemia. | the expression of lymphocyte surface markers as well as the production of interleukin-2 (il-2) and interferon-gamma (ifn) by mitogen-stimulated peripheral blood mononuclear cells (mnc) have been studied in five children with constitutional aplastic anemia. a significantly reduced t4/t8 ratio was found and two of five patients also had a reduced percentage of b cells. one patient had a high percentage of hla-dr positive t8+ cells, very suggestive of a high degree of circulating activated t suppre ... | 1988 | 2484652 |
| genes within genes: independent expression of phage t4 intron open reading frames and the genes in which they reside. | the td, nrdb, and suny introns of bacteriophage t4 each contain a long open reading frame (orf). these orfs are preceded by functional t4 late promoters and, in the case of the nrdb intron orf, a functional middle promoter. expression of phage-encoded intron orf-lacz fusions indicates that these t4 genes are highly regulated. the lack of translation of these orfs from early pre-mrnas can be accounted for by the presence of secondary structures that are absent from the late rnas. because translat ... | 1988 | 2467840 |
| symmetric transcription of bacteriophage t4 base plate genes. | dot-blot and northern-blot experiments, using strand-specific rna probes, show that part of the bacteriophage t4 dna that codes for six of the base plate structural genes (gp 51, 27, 28, 29, 48 and 54), is transcribed in vivo from both dna strands. the r dna strand transcripts contain sequences which are translated into structural proteins. antisense l strand rna is about 100 fold less abundant than rna molecules transcribed from the r dna strand. | 1988 | 2468563 |
| stability of group i intron rna in escherichia coli and its potential application in a novel expression vector. | intron rna excised from the primary transcript of the phage t4 td gene was found to be unusually stable in vivo. in contrast to the average half-life of about 1.5 min for a typical escherichia coli mrna at 37 degrees c, the half-life of the excised group-i td intron ranged from 12 to 19 min for the linear form and from 22 to 33 min for the circular form. a 631-nucleotide region of the intron that is not essential for splicing was replaced by the chloramphenicol acetyltransferase (cat) structural ... | 1988 | 2468580 |
| alpha-subunit secretion in men with idiopathic hypogonadotropic hypogonadism. | we studied the regulation of glycoprotein hormone alpha-subunit secretion in four men with idiopathic hypogonadotropic hypogonadism due to presumed gnrh deficiency. immunoreactive alpha-subunit was present at low but usually detectable levels in blood samples drawn at 10- to 20-min intervals for 12-24 h; however, no characteristic pattern of pulsatile alpha-subunit secretion was found. serum from each man was examined by gel filtration chromatography. each sample tested contained an immunoreacti ... | 1988 | 2448330 |
| antigenic determinants on thyroglobulin: comparison of the reactivities of different thyroglobulin preparations with serum antibodies and t cells of patients with chronic thyroiditis. | to delineate the antigenic determinants on thyroglobulin (tg) recognized by serum autoantibodies and peripheral blood t cells from patients with chronic thyroiditis, we studied the reactivities of three different tg preparations, i.e. enzyme-digested tg fragments, physically or chemically denatured tg, or tg with differing iodine contents. human tg was digested with staphylococcal v8 protease, and the fragments were separated by high performance liquid chromatography. the autoantibodies reacted ... | 1988 | 2450103 |
| human epidermal keratinocytes in culture convert thyroxine to 3,5,3'-triiodothyronine by type ii iodothyronine deiodination: a novel endocrine function of the skin. | cultured human keratinocytes converted t4 to t3 by type ii iodothyronine deiodination. homogenates of keratinocytes cultured from neonatal foreskin or adult arm skin had similar mean t4 5'-deiodinating activities. conversion of t4 to t3 by intact cells was demonstrable in cultures from neonatal and adult donors. only phenolic ring deiodination occurred in the cultured cells and their homogenates, the apparent michaelis constant for t4 was 12 nmol/l, and t4 and rt3 each inhibited 5'-deiodination ... | 1988 | 2450104 |
| induction cytotoxic chemotherapy: the significance of apparent complete clinical regression after using the price hill schedule a protocol for squamous carcinoma of the head and neck. | a small group of 46 previously untreated patients out of about 260 in all who have received price hill schedule a cytotoxic chemotherapy, achieved apparent complete clinical regression (accr) of their disease before local treatment. forty patients were classified t3 or t4, over half having clinically positive nodes at the start of treatment. a determinate survival rate of 70 per cent disease free at three years was obtained and 74 per cent achieved quality relief of all symptoms. two patients de ... | 1988 | 2465362 |
| induction of the heat shock regulon of escherichia coli markedly increases production of bacterial viruses at high temperatures. | production of bacteriophages t2, t4, and t6 at 42.8 to 44 degrees c was increased from 8- to 260-fold by adapting the escherichia coli host (grown at 30 degrees c) to growth at the high temperature for 8 min before infection; this increase was abolished if the host htpr (rpoh) gene was inactive. others have shown that the htpr protein increases or activates the synthesis of at least 17 e. coli heat shock proteins upon raising the growth temperature above a certain level. at 43.8 to 44 degrees c ... | 1988 | 2446014 |
| the influence of a double-stranded hindrance on dna synthesis performed by dna polymerase alpha, t4 dna polymerase, dna polymerase i (klenow fragment) and amv reverse transcriptase. | the influence of a double-stranded region on dna synthesis performed by a series of dna polymerases on a single-stranded template was studied. two types of double-stranded hindrances were employed: a stable hairpin formed by the template alone and a region formed by the template and an extraneous oligonucleotide complementary to the template. while t4 and calf thymus alpha dna polymerases are strongly arrested at the beginning of either of the two double-stranded hindrances, the klenow fragment ... | 1988 | 2449362 |
| thymus in myasthenia gravis. isolation of t-lymphocyte lines specific for the nicotinic acetylcholine receptor from thymuses of myasthenic patients. | the thymus is believed to play a central role in the pathogenesis of myasthenia gravis (mg). according to a previous hypothesis, mg is initiated within the thymus by immunogenic presentation of locally produced nicotinic acetylcholine receptor (achr) to potentially autoimmune t cells. data of 10 consecutive mg patients demonstrate two critical features of mg thymuses that support the concept of intrathymic activation of autoreactive, achr-specific lymphocytes. morphologically, the thymuses showe ... | 1988 | 2449461 |
| the mechanisms of action of e. coli endonuclease iii and t4 uv endonuclease (endonuclease v) at ap sites. | treatment of dna containing ap sites with either t4 uv endonuclease or with e. coli endonuclease iii followed by a human class ii ap endonuclease releases a putative beta-elimination product. this result suggests that both the t4 endonuclease and e. coli endonuclease iii class i ap endonucleases catalyze phosphodiester bond cleavage via a lyase- rather than a hydrolase mechanism. indeed, we have not detected a class i ap endonuclease which hydrolytically catalyzes phosphodiester bond cleavage. w ... | 1988 | 2449657 |
| cuucgg hairpins: extraordinarily stable rna secondary structures associated with various biochemical processes. | the mrna of bacteriophage t4 contains a strikingly abundant intercistronic hairpin. within the 55 kilobases of known t4 sequence, the hexanucleotide sequence cttcgg is found 13 times in the dna strand equivalent to mrna sequences. in 12 of those occurrences, the sequence is flanked by inverted repeats predictive of rna hairpins with uucg in the loop. avian myeloblastosis virus reverse transcriptase, which can traverse hairpins of larger calculated stability, terminates efficiently at these cuucg ... | 1988 | 2449689 |
| antiviral effect of recombinant gamma interferon in chronic hepatitis b virus infection: a pilot study. | a pilot study has been designed in order to determine the tolerance and effectiveness of recombinant interferon gamma (rifn-gamma) in chronic hepatitis b virus (hbv) infection. eight hbsag, hbeag, hbv-dnap and hbv-dna-positive patients were randomly assigned to one of two groups. in group 1, 0.25 mg of rifn-gamma/m2 body surface was administered daily for 28 days to 4 patients. in group 2, 0.10 mg of rifn-gamma was administered to 4 patients under the same conditions. all the patients concluded ... | 1988 | 2452123 |
| interferons (ifns) and tumor necrosis factors (tnfs) in t cell-mediated immune responses against alloantigens. i. influence on the activation of resting and antigen-primed t cells. | the aim of this study was to investigate the influence that endogenous ifns released in response to antigenic or viral stimuli has on recognition of alloantigens in mlc. results indicated that both the magnitude and the kinetics of response can be modified by ifns. neutralizing antibodies with specificity for ifn-gamma inhibit early and enhance late proliferative responses in mlc. addition of physiological concentrations of ifn-gamma enhanced both early and peak proliferation, whereas ifn-alpha ... | 1988 | 2452129 |
| the lit gene product which blocks bacteriophage t4 late gene expression is a membrane protein encoded by a cryptic dna element, e14. | escherichia coli lit(con) mutations cause a severe inhibition of gene expression late in infection by bacteriophage t4 owing to the overproduction of one, and possibly two, proteins (c. kao, e. gumbs, and l. snyder, j. bacteriol. 169:1232-1238, 1987). one or both of these proteins interact, either directly or indirectly, with a short sequence about one-quarter of the way into the major capsid protein gene of t4, and the inhibition occurs when this late gene of the virus is expressed. in this rep ... | 1988 | 2452152 |
| [immunological profile of whipple's disease evolving over a period of 17 years]. | this report describes an immunological study made on a 58 years old patient with a whipple disease diagnosed in 1969 and treated with different antibiotics. all attempts to stop the antibiotherapy resulted in reappearance of clinical symptoms. further, this patient suffered anguillulosis infection in 1954 and this persists despite thiabendazole therapy, as shown by periodical creeping lunear dermatitis (larva currens). laboratory investigations displayed low igm levels and lack of cutaneous reac ... | 1988 | 2452591 |
| hiv-1 expression by t8 lymphocytes after transfection. | acquired immune deficiency syndrome (aids) is an immunosuppressive disease associated with the depletion of t4 lymphocytes. recently, an hiv-1 genome was molecularly cloned and shown to be fully infectious in vitro by transfection experiments. in this study, we show that hiv-1 can be transfected into t4 and t8 lymphocyte subpopulations and viral proteins and infectious virus particles are observed in both short- and long-term cultures. in addition, transfected t8 cells can be maintained in cultu ... | 1988 | 2452649 |
| activation of t lymphocytes by immobilized monoclonal antibodies to cd3. regulatory influences of monoclonal antibodies to additional t cell surface determinants. | the effect of soluble or immobilized mab directed at various additional surface proteins on the proliferation of highly purified t4 cells induced by two immobilized mab to cd3, okt3 and 64.1, was examined. high density 64.1 stimulated nearly all t4 cells to enter and progress through the cell cycle. maximal t4 cell proliferation required stimulation with immobilized 64.1 throughout the length of the incubation and was not effected by any of the additional soluble or immobilized mab employed. in ... | 1988 | 2452835 |
| dependence of m1 rna substrate specificity on magnesium ion concentration. | we have constructed a plasmid expressing e. coli m1 rna, the catalytic rna subunit of ribonuclease p, under the control of a phage t7 promoter. the active m1 rna species synthesized in vitro by t7 rna polymerase from this vector was reacted with the trna(gln) - trna(leu) precursor rna (band k) encoded by phage t4. only the trna(leu) moiety of this dimeric precursor rna contains the 3' terminal c-c-a sequence common to all trnas. we observed that protein-free m1 rna was capable of processing the ... | 1988 | 2453026 |
| hypothyroidism increases substance p concentrations in the heterotopic anterior pituitary. | the regulatory effects of thyroid hormone on adenohypophysial substance p (sp) were studied in heterotopically implanted anterior pituitaries. three or four anterior pituitaries from 21-day-old rat pups were implanted under the renal capsule in 175- to 200-g adult rats. the donor and recipient animals were sex matched. one week after implantation, animals were thyroidectomized or sham operated. a separate group of animals received daily t4 treatment (1.5 g/100 g, ip). after 2 weeks, the native a ... | 1988 | 2453352 |
| the organization of titin filaments in the half-sarcomere revealed by monoclonal antibodies in immunoelectron microscopy: a map of ten nonrepetitive epitopes starting at the z line extends close to the m line. | mabs specific for titin or nebulin were characterized by immunoblotting and fluorescence microscopy. immunoelectron microscopy on relaxed chicken breast muscle revealed unique transverse striping patterns. each of the 10 distinct titin antibodies provided a pair of delicate decoration lines per sarcomere. the position of these pairs was centrally symmetric to the m line and was antibody dependent. the results provided a linear epitope map, which starts at the z line (antibody t20), covers five d ... | 1988 | 2453516 |
| immunohistochemistry of lymphocytes and langerhans' cells in long-lasting allergic patch tests. | a long-lasting allergic patch test is a "normal" allergic patch test that remains positive for weeks or months. an immunohistochemical study of immunocompetent cells in the skin in this rare type of patch tests was performed. most inflammatory cells were t11 positive t-lymphocytes. the majority of these cells were of the helper/inducer phenotype (t4+), but a relative increase of t8+ cells as compared to the initial (1-2d) stages of allergic patch tests was observed. t6+ langerhans' cells (lcs) w ... | 1988 | 2453989 |
| anti-cd4 and anti-cd2 monoclonal antibody infusions in subjects with multiple sclerosis. immunosuppressive effects and human anti-mouse responses. | the immunologic responses to anti-t4 and anti-t11 mab infusions were assessed in subjects with chronic progressive multiple sclerosis as part of phase i clinical studies. eight subjects received five daily infusions (0.2 mg/kg/day) of either anti-t11 (cd2) or anti-t4 (cd4) murine mab. it was found that in vivo anti-t cell mab infusions specifically suppress in vitro measurements of the human immune response; anti-t11 mab blocked t cell activation via the cd2 srbc-binding protein, and anti-t4 mab ... | 1988 | 2454256 |
| neoadjuvant chemotherapy in previously untreated patients with advanced head and neck squamous cell cancer. | between may 1981 and january 1986, 130 consecutive patients with advanced untreated head and neck squamous cell cancer (hnscc) received neoadjuvant chemotherapy (nac) with two or three courses of the cabo combination (methotrexate 40 mg/m2 intravenously (iv) on days 1 and 15; cddp 50 mg/m2 iv on day 4; bleomycin 10 mg iv on days 1, 8, and 15; and vincristine 2 mg iv on days 1, 8, and 15 every 3 weeks) prior to surgery and/or radiotherapy. of the 123 patients evaluable for response to chemotherap ... | 1988 | 2454721 |
| characterization of ion channels involved in the penetration of phage t4 dna into escherichia coli cells. | the hypothesis of a channel-mediated transport of phage dna into escherichia coli cytoplasmic membrane has been formulated for a long time. in this paper, we present experimental evidence in favor of this proposal. we have analyzed the kinetics of the k+ efflux induced by t4 phage and ghosts (phage depleted of dna) using a potassium selective electrode. we show that the k+ efflux is not catalyzed by the k+ transport systems. the km of k+ efflux is the same for phage and ghosts. the rate of k+ ef ... | 1988 | 2454920 |
| origin of sympathetic and sensory innervation of the elbow joint in the rat: a retrograde axonal tracing study with wheat germ agglutinin conjugated horseradish peroxidase. | after injection of wheat germ agglutinin conjugated horseradish peroxidase (wga-hrp) into the elbow joint of adult rats, labeled neurons were found in the stellate and the t2-t4 ganglia of the ipsilateral sympathetic trunk, and also in dorsal root ganglia at the c4-t4 levels. most labeled sympathetic cells, 90% or more, were located in the stellate ganglion. the sensory innervation to the joint originated mainly from the dorsal root ganglia at the levels of c7-t1. | 1988 | 2454963 |
| degradation of structurally modified dnas by bleomycin group antibiotics. | bleomycin-mediated dna strand scission has been shown to be diminished at certain sequences in proximity to 5-methylcytidines. we have investigated the molecular basis of this observed diminution using selective bleomycin (blm) modifications at the c-terminus. of the four different bleomycin congeners investigated, only bleomycin a2 and bleomycin bapp were substantially affected by cytidine methylation. we have also examined the effect of other dna modifications on bleomycin-mediated strand scis ... | 1988 | 2455539 |
| the effect of altered thyroid function on serum fructosamine concentrations. | the effect of altered thyroid function on serum fructosamine concentrations was investigated in 31 untreated hyperthyroid patients, 18 short-term hypothyroid patients (i.e., 20 days after withdrawal of thyroid hormone suppressive therapy for thyroid cancer), 7 untreated long-term hypothyroid patients, and 25 age-matched normal controls. no differences in serum fructosamine concentrations were observed between hyperthyroid patients and normal controls; conversely, serum fructosamine concentration ... | 1988 | 2455610 |
| an okazaki piece of simian virus 40 may be synthesized by ligation of shorter precursor chains. | it is generally accepted that an aphidicolin-sensitive dna polymerase elongates the eucaryotic rna primer (irna) into a mature okazaki piece reaching ca. 200 nucleotides. yet, as shown here, nascent dna chains below 40 nucleotides accumulated in simian virus 40 (sv40) dna replicating in isolated nuclei in the presence of aphidicolin. these products resembled precursors of longer okazaki pieces synthesized in the absence of aphidicolin (termed here dna primers) in size distribution, lagging-repli ... | 1988 | 2455822 |
| the cd4 receptor is complexed in detergent lysates to a protein-tyrosine kinase (pp58) from human t lymphocytes. | the cd4 (t4) antigen is a cell-surface glycoprotein that is expressed predominantly on the surface of helper t cells and has been implicated in the regulation of t-cell activation and in the associative recognition of class ii antigens of the major histocompatibility complex. in addition, the cd4 antigen appears to serve as a receptor for the human immunodeficiency virus (hiv). an important question has been whether the cd4 receptor is linked to an intracellular mediator that could regulate the ... | 1988 | 2455897 |
| characterization of thyroid hormone effects on na-k pump and membrane potential of cultured rat skeletal myotubes. | the purpose of this study was to characterize the effects of thyroid hormone on the na-k pump and resting membrane potential (em) of rat skeletal myotubes in culture. myotubes were obtained from fetal (19-21 day) or neonatal rats (1-2 day) by serial trypsinization and maintained in culture for up to 10 days. cells were treated with t4 or t3 on day 6 or 7, and measurements were made of em, [3h]ouabain binding, and ouabain-sensitive 86rb uptake at various times thereafter. hormone treatment increa ... | 1988 | 2456206 |
| [a case of chemical hyperthyroidism induced by antiarrhythmic agent amiodarone]. | amiodarone, an antiarrhythmic agent, is known to occasionally induce alterations in thyroid function because of its iodine content and ability to inhibit t4 5'-monodeiodination. we herein describe the drug-induced chemical hyperthyroidism in a diabetic patient with ventricular premature beats. a 46-year-old man with well controlled diabetes mellitus revealed neck swelling during a 4 months' treatment with amiodarone for his frequent occurrence of ventricular premature beats. physical findings we ... | 1988 | 2456231 |
| detection of an epitope, not required for polymerization, that is conserved between e.coli dna polymerases i and iii and bacteriophage t4 dna polymerase. | monoclonal antibodies directed against the alpha subunit of the dna polymerase iii holoenzyme (1) of e. coli were tested for cross-reactivity with a variety of polymerases. we found that one monoclonal antibody bound to e. coli dna polymerase i as well as to dna polymerase iii. a weaker, but specific, interaction was also detected with t4 dna polymerase. we exploited the proteolysis procedure developed by setlow, brutlag and kornberg (2) to determine which domain of dna polymerase i contained th ... | 1988 | 2456526 |
| the immunological profile in sickle cell anaemia: a study of patients in the arab peninsula. | recurrent and often serious infections are common in sickle cell anaemia. the predisposing causes are multiple, and immune abnormalities are frequently blamed. in this study, immune complexes, lymphocyte subpopulations, immunoglobulins and complement were determined in 40 arabs with sickle cell anaemia to ascertain some aspects of their immunological profile. immunoglobulins were found to be either normal or high, c3 and c4 values mostly clustered at the lower level of normal with a few values b ... | 1988 | 2456728 |
| analysis of cleavage products of dna repair enzymes and other nucleases. characterization of an apurinic/apyrimidinic specific endonuclease from mouse plasmacytoma cells. | we have developed a strategy by which the nature of phosphodiester bond breaks produced by various dna-repair endonucleases and also other nucleases, can be characterized. a purified apurinic/apyrimidinic (ap) specific endonuclease from a permanently established mouse plasmacytoma cell-line (mpc-11) has been examined with respect to the exact incision site generated at the baseless site. by the aid of enzymatic treatment with calf intestinal phosphatase, the 3'-phosphatase activity of t4-polynuc ... | 1988 | 2457203 |
| effect of preoperative iodine in patients with graves' disease controlled with antithyroid drugs and thyroxine. | thirty-four patients with graves' disease, first rendered euthyroid with antithyroid drugs (atd) then given supplementary thyroxine (t4), were randomly allocated to three treatment groups. in group i atd and t4 were stopped 10 days before partial thyroidectomy and the patients were treated with lugol's iodine alone. in group ii the patients were treated up to the time of operation with atd and t4 alone. in group iii atd and t4 were continued until the day of operation, but the patients also rece ... | 1988 | 2457351 |
| characterization of human t8+ suppressor and contrasuppressor cells, separated by the lectin vicia villosa. | the helper function of human t4+ cells acting on autologous b cells, in the presence of monocyte-enriched cells (mec), has been studied using the hapten-carrier conjugate dnp-streptococcal antigen (dnp-sa). the antibody response can be suppressed by t8+ cells, but selection of a vicia villosa lectin-non-adherent (t8vv-) subset enhances the suppressor function. the t8 vicia villosa lectin-adherent (t8vv+) cells are not suppressive, rather they inhibit the t8vv- cell suppressor function. sequentia ... | 1988 | 2450835 |
| differential regulation of interleukin 2 (il-2) receptor expression on antigen-specific human t cell clones by il-2. | a panel of human t cell clones bearing exclusively the t4 (helper) phenotype and demonstrating specificity to a well-characterized soluble glycoprotein antigen (185,000 dalton streptococcal antigen, sa) is described. after having been cultured in exogenous interleukin 2 (il-2) for 7 days in the absence of the specific antigen, two of the clones, namely sa1.4 and sa1.23, show stronger proliferative responses to the ligand as compared to the other t4 clones. analysis of both the high- and low-affi ... | 1988 | 2450841 |
| acute and late toxicity associated with sequential bleomycin-containing chemotherapy regimens and radiation therapy in the treatment of carcinoma of the nasopharynx. | between 1975 and 1984, 33 patients with squamous cell carcinoma of the nasopharynx received adjuvant chemotherapy before and/or after definitive radiotherapy at ut m. d. anderson hospital. the favored chemotherapy regimens during this time were bcmf (bleomycin, cyclophosphamide, methotrexate, and 5-fu) and pmb (cisplatinum, methotrexate, and bleomycin). total radiation doses to the primary site averaged 65 gy for t1 and t2 lesions and 70 gy for t3 and t4 lesions. neck nodes were given boost trea ... | 1988 | 2450858 |
| activation of human t4 cells by cross-linking class i mhc molecules. | these studies examined whether cross-linking class i mhc molecules results in functional or biochemical responses in human t4 cells. the initial studies demonstrated that cross-linking class i mhc molecules either by culturing highly purified t4 cells with immobilized mab to class i mhc ag or reacting the t4 cells with mab to class i mhc ag and then cross-linking the mab with goat antimouse ig (gamig) enhanced t4 cell proliferation induced by an immobilized mab to cd3, okt3. more-over, immobiliz ... | 1988 | 2450913 |
| ten-year results of randomized trial comparing radiotherapy and concomitant bleomycin to radiotherapy alone in epidermoid carcinomas of the oropharynx: experience of the european organization for research and treatment of cancer. | this trial of treatment for head and neck carcinoma was initiated in 1973 by the european organization for research and treatment of cancer. its purpose was to investigate the value of single-agent chemotherapy with bleomycin (blm) given during the course of a conventional treatment by external radiotherapy (rt) compared to treatment by external rt alone. in this randomized study, we compared treatment results in 2 groups of patients with squamous cell carcinoma of the oropharynx (t2, t3, and t4 ... | 1988 | 2451135 |
| human immunodeficiency virus replication in human brain cells. brief report. | human immunodeficiency virus (hiv), the etiologic agent of aids, was found to infect and replicate in human brain cells. the extent of hiv replication was minimal in human brain-derived cells in comparison to t4 lymphoid cells. these results suggest that direct infection of glial/neuronal cells by hiv may contribute to the cns dysfunction frequently observed in hiv infected individuals. | 1988 | 2451491 |
| sequence-specific recognition and cleavage of dna by metallobleomycin: minor groove binding and possible interaction mode. | the dnase i cleavage-inhibition analysis shows binding sites of approximately 2 or 3 base pairs--in particular, 5' n-g-c sequences--for the green-colored coiii and fully oxidized feiii complexes of bleomycin. the apparent binding constant of the bleomycin-coiii complex is quite similar for glucosylated and nonglucosylated phage t4 dnas, whereas poly[d(i-c)] clearly gives a smaller binding constant than does poly[d(g-c)]. in contrast to the covalent attachment of the guanine n-7 with aflatoxin b1 ... | 1988 | 2451823 |
| regional specificities of monoclonal anti-human apolipoprotein b antibodies. | the usefulness of monoclonal antibodies as probes of protein structure is directly related to knowledge of the structures and locations of the epitopes with which they interact. in this report we provide a detailed map of 13 epitopes on apob-100 defined by our anti-apob monoclonal antibodies based on current information on the amino acid sequence of apob-100. to localize antibody specificities to smaller regions along the linear sequence of the apob-100 molecule we used a) thrombin- and kallikre ... | 1988 | 2457642 |
| protein synthesis and quantitative morphology in thyroid tissue from hyperthyroid patients after preoperative treatment with antithyroid or beta-adrenergic antagonist drugs. | the rate of protein synthesis was measured in thyroid tissue obtained from three groups of patients undergoing thyroid surgery: group i (n = 18), hyperthyroid patients preoperatively treated with an antithyroid drug and t4; group ii (n = 11), hyperthyroid patients preoperatively treated with a beta-adrenergic antagonist drug; and group iii (n = 9), euthyroid patients operated on for multinodular goiter or adenoma. quantitative morphology was studied in the resected thyroid tissue from the patien ... | 1988 | 2458376 |
| functional consequences of the arabinosylcytosine structural lesion in dna. | cytosine arabinoside (arac) is a potent antileukemic agent that is misincorporated into dna in the course of its action. we have developed a chemical synthetic method that allows site-specific introduction of arac into synthetic dna oligomers. we describe here the utilization of these oligomers as primer/template substrates for in vitro dna synthesis reactions and as fragments for dna ligation. these studies were undertaken to investigate the manner in which sites of arac misincorporation consti ... | 1988 | 2458756 |
| antiproteases are increased in bronchoalveolar lavage in interstitial lung disease. | the present study evaluates different cellular and soluble components in the bronchoalveolar lavage (bal) from patients with interstitial lung disease. we observed an increased t4/t8 lymphocyte ratio in bal but not in blood from 24 patients with active pulmonary sarcoidosis compared to sixteen normal individuals and to eleven patients with inactive pulmonary sarcoidosis. seven patients with hypersensitivity pneumonitis had a normal t4/t8 ratio. in the active sarcoidosis and hypersensitivity pneu ... | 1988 | 2458961 |
| comparative removal of pyrimidine dimers from human epidermal keratinocytes in vivo and in vitro. | we have compared the kinetics for repair of uv-induced cyclobutane pyrimidine dimers in the dna of keratinocytes in human skin and in cell culture. a small area of the buttocks of volunteers was exposed to uvb-irradiation and biopsies were taken at various time intervals. epidermal keratinocytes in culture from the same subjects were exposed to uvc with doses chosen to elicit comparable yields of dimers in cellular dna. the initial density of pyrimidine dimers and the kinetics of their removal w ... | 1988 | 2459263 |
| lipolysis of ldl with phospholipase a2 alters the expression of selected apob-100 epitopes and the interaction of ldl with cells. | to assess the effects of perturbing the surface of low density lipoprotein (ldl) on the conformation of apob-100, ldl (d 1.030-1.050 g/ml) isolated from normal subjects were treated with phospholipase a2 (pl-a2) for 0.5 to 15 min. the resulting p-ldl and concurrent control ldl (c-ldl) incubated without pl-a2 were isolated by gel permeation chromatography. approximately 50% of ldl-phosphatidylcholine was hydrolyzed in 2 min and approximately 85% in 5 min. lysophosphatidylcholine compounds (lpc) a ... | 1988 | 2459282 |
| a novel procedure for selective cloning of noti linking fragments from mammalian genomes. | a novel procedure has been developed for selective cloning of noti linking fragments from mammalian genomes. since the majority of the noti sites in mammalian genomes are considered to be localized in so-called htf (hpaii tiny fragment) islands, an htf library was constructed as an initial step to enrich the noti sites. the plasmid dnas were isolated en masse from the htf library and digested with noti. linearized plasmid dnas derived from noti linking clones were efficiently separated from undi ... | 1988 | 2459661 |
| [changes in the hormone content of the blood during chronic electrical stimulation of the midbrain]. | the levels of hormones of the hypophysis (acth, tth, cth), thyroid gland (t3, t4), adrenal glands (epinephrine, norepinephrine, cortisol) and pancreas (insulin, glucagon) increased during a prolonged electrostimulation of the reticular nucleus of mesencephalon in rabbits. administration of the substance p returned the hormonal levels to normal. | 1988 | 2460380 |
| the different effect of alpha and gamma interferons and interleukin 2 on the expression of cd2, cd3, cd4 and cd8 antigens in comparison to histocompatibility antigens of human lymphocytes. | the effects of alpha- and gamma-interferons (ifn-alpha, -gamma) and of interleukin 2 (il-2) on the expression of certain differentiation antigens were compared with those of major histocompatibility antigens on human lymphocytes. ifn-gamma and ifn-alpha in high doses significantly increased the expression of t11 (cd2) differentiation antigen, but did not affect the expression of t4 (cd4), t8 (cd8), t3 (cd3) and leu-7 antigens (hnk-1). both natural and recombinant ifn-alpha and -beta apparently i ... | 1988 | 2460402 |
| xenopus transcription factor iiia-dependent dna renaturation. | kinetic and titration analyses are used to elucidate the mechanism by which xenopus transcription factor iiia (tfiiia), a protein required for 5 s rna synthesis by rna polymerase iii, promotes dna renaturation. tfiiia promotes 50% renaturation of complementary strands (303 bases) in 45 s. analyses of the renaturation kinetics indicate the rate-limiting step in this tfiiia-dependent reaction is first order. tfiiia-dependent dna renaturation is a stoichiometric rather than a catalytic process. the ... | 1988 | 2460459 |
| rapid and automated tetrazolium-based colorimetric assay for the detection of anti-hiv compounds. | a rapid, sensitive and automated assay procedure was developed for the in vitro evaluation of anti-hiv agents. an htlv-i transformed t4-cell line, mt-4, which was previously shown by koyanagi et al. (1985) to be highly susceptible to, and permissive for, hiv infection, served as the target cell line. inhibition of the hiv-induced cytopathic effect was used as the end point. the viability of both hiv- and mock-infected cells was assessed spectrophotometrically via the in situ reduction of 3-(4,5- ... | 1988 | 2460479 |
| the effects of lesions of superior temporal gyrus and inferior parietal lobe on temporal and vertex components of the human aep. | we recorded auditory evoked potentials (aeps) to 1 khz tone bursts in controls and patients with unilateral lesions centered in posterior superior temporal gyrus and adjacent caudal inferior parietal lobule (stg) or in rostral inferior parietal lobule (ipl). controls generated a vertex maximal n94 (n1b) and p200 (p2) and additional p45, n78 and n127 temporal aep components (p45, n1a, n1c). similar to prior reports, in controls the n1a was most prominent over the left temporal lobe and the p45 wa ... | 1988 | 2461284 |
| [ambivalent effect of thyroxine on the expression of gonadotropin genes in normal and orchidectomized rats]. | in order to examine the role of thyroid hormones on pituitary gonadotropin synthesis and release, normal, thyroidectomized and orchidectomized male rats were treated in parallel by daily injections of thyroxine (10 micrograms/10 g). pituitary levels of alpha, lh beta, fsh beta and tsh beta mrna were determined using two complementary approaches: 1) cell-free translation of pituitary mrna and quantitative immunoprecipitation of labeled subunit precursors, and 2) hybridization with [32p]-labeled c ... | 1988 | 2462206 |
| evaluation of thyroid gland activity by hormone assays and flow cytometry in rats. | spontaneous variations in the activity of thyroids and changes induced by methimazole, phenobarbital and 2,4--diaminoanisole sulfate were assessed in rats by repeated measurements of serum t3, t4 and tsh concentrations and cell cycle analysis in isolated nuclei of the thyroids. methimazole caused a decrease in t3 and t4, and elevation in tsh, reduction of cells in g1 and increase in the percentage of cells in s and g2 phases. with phenobarbital t4 concentrations were decreased, but a mitogenic e ... | 1988 | 2462517 |
| t4 to t2 resistivity transition and superconducting fluctuations in disordered vn films. | 1988 | 9946920 | |
| clinical evaluation of thyroid function in racing pigeons (columba livia domestica). | reference values for basal thyroxine (t4) concentrations in racing pigeons are presented for use in clinical pathology. values for basal plasma t4 concentrations in healthy racing pigeons over the four seasons were 6-35 nmol/litre. significant differences were seen between seasons. to differentiate between primary hypothyroidism and other causes of lowered plasma t4 concentrations the optimum dose and sampling times for a tsh stimulation test in this species were determined. a convenient applica ... | 1988 | 18766667 |
| spinal anesthesia with pentazocine for total abdominal hysterectomy. | intraspinal pentazocine, 1.5 mg/kg, produced sufficient analgesia and moter block in 50 cases of abdominal total hysterectomy. pentazocine, a derivative of benzomorphans, shares some common characteristics with local anesthetics in the chemical structure. both agents have an aromatic ring and an intermediate chain, in common, which connects the former to nitrogen moiety, and the local anesthetic action was anticipated. the onset of the sensory block was 3.2 +/- 0.9 (mean +/- sd) min, and the max ... | 1988 | 15235841 |
| feline hyperthyroidism--a review. | feline hyperthyroidism is the name given to the multisystemic manifestation of excessive concentrations of circulating thyroid hormones, thyroxine (t4) and tri-iodothyronine (t3). the most common cause is a functional thyroid adenoma involving one or both thyroid lobes. both multinodular adenomatous hyperplasia and multinodular adenomatous goitre are acceptable descriptions for the histopathological findings. thyroid adenocarcinoma rarely causes hyperthyroidism in the cat, and when present, seld ... | 1988 | 16031447 |
| genomic diversity of zairian hiv isolates: biological characteristics and clinical manifestation of hiv infection. | we describe here several african isolates of hiv, compare them to u.s.-european prototype isolates and to each other, correlate the number of isolates with serological results, and provide insights into the disease spectrum associated with hiv infection in africa. three of 25 healthy zairian donors and 54 of 87 zairian patients selected for specific pathology and hospitalized in the internal medicine department of the university clinic of kinshasa, zaire, were hiv positive over a six month perio ... | 1987 | 2451928 |
| [determining the triiodothyronine uptake and its derivative values-t4, t3 and tbg indices-based on labeled t3 binding by a mixture of activated charcoal and cellulose]. | 1987 | 2457494 | |
| seasonal peculiarities of thyroxine influence on the way of serotonin metabolism in the pineal gland of the rat. | in intact wistar pubertal male rats held on ld 8:16 (winter) and 16:8 (summer) pineal melatonin (m) production and other pineal indoles content serotonin (s), 5-methoxytriptamine (5-mt), n-acetylserotonin (n-as), 5-hydroxyindoleacetic acid (5-hiaa) and 5-methoxyindoleacetic acid (5-miaa) were investigated in basic conditions and after 10-days t4 administration. the results suggest, that intact control in winter comparing with summer demonstrates high m, n-as, 5-hiaa and 5-miaa level, but low 5-m ... | 1987 | 2449798 |
| [change in serum thyroid hormone levels in patients with acute myocardial infarction]. | it has been reported that there is a decrease in the serum concentration of thyroid hormones in non-thyroidal illness. in the present study we made serial measurements of serum concentration of thyroid hormones [triiodothyronine (t3), thyroxine (t4), free triiodothyronine (ft3), free thyroxine (ft4), reverse triiodothyronine (rt3)], thyroid stimulating hormone (tsh) and thyroxine binding globulin (tbg) in 10 patients with acute myocardial infarction (ami, grade i, according to the classification ... | 1987 | 2435582 |
| [studies on cytosol thyroid hormone binding proteins in the rat liver: part i. stability and binding characteristics of thyroid hormone binding proteins]. | many studies demonstrated the presence of cytosolic thyroid hormone binding proteins (cthbps) in various tissues, but the physiologic significance of these cthbps is not clear, partially because of the lack of information about the physicochemical properties of cthbps as purified forms. since the difficulty in isolating these cthbps is considered to be due to instability during the various procedures for their isolation, studies on the stability of cthbps of rat liver were performed using a char ... | 1987 | 2435583 |