Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| lipolytic action of cholera toxin on fat cells. re-examination of the concept implicating gm1 ganglioside as the native membrane receptor. | the possible role of galactosyl-n-acetylgalactosaminyl-[n-acetylneuraminyl]-galactosylglucosylceramide (gm1) ganglioside in the lipolytic activity of cholera toxin on isolated fat cells has been examined. analyses of the ganglioside content and composition of intact fat cells, their membranous ghosts, and the total particulate fraction of these cells indicate that n-acetylneuraminylgalactosylglucosylceramide (gm3) represents the major ganglioside, with substantial amounts of n-acetylgalactosamin ... | 1976 | 1002701 |
| [the protective and immunodepressive activity of cell fractions of a cholera-like vibrio]. | a study was made of the protective and immunodepressive activity of the sytoplasmic fractions of a cholera-like vibrio. ribosomal fraction proved to possess more marked protective and immunodepressive properties than the soluble cytoplasmic fraction. | 1976 | 1007718 |
| [a study of immunological efficacy of the oral chemical cholera vaccine on experimental animals]. | three fractions isolated from the culture fluid of the 569b cholera vibrio strain were studied. the fractions differed by the extent of purification, and the content of the toxid and the o-antigen. in intraintestinal application to rabbits all of them caused formation of antitoxins and vibriocidal antibodies in the blood serum. the immunizing dose of the preparations in intraintestinal administration exceeded the dose required for subcutaneous application. fraction i should be used for producing ... | 1976 | 1007720 |
| [a comparative study of ultrastructural organization of the nonagglutinating vibrios and the causative agents of cholera]. | a common plan of ultrastructural organization was revealed in a comparative study of the ultrastructure of the nonagglutinating el tor and cholera vibrios: the presence of a three-layer cell wall and cytoplasmic membrane, the presence of vesicles on the external layer of the cell wall or between the cell wall and the cytoplasmic membrane; identity of the intracytoplasmic membranous structures; the presence of intracytoplasmic inclusions in the form of granules of various size and electron densit ... | 1976 | 1007727 |
| [experimentally induced cholera in guinea pigs. i. elaboration of the method of infection]. | the method of intrapulmonary infection of guinea pigs was suggested for the assessment of the virulent properties of cholera vibrios. addition into the diluent of 10% peptone, 10% gelatine and 0.05% agar-agar led to the reduction of ld50 by over 1000 times. a specific infectious process coursing in an acute generalized form with bacteriemia and affection of the small intestine developed in the infected animals. the majority of the animals perished in 1 to 2 days. | 1976 | 1007732 |
| sequence of events in the activation of adenylate cyclase by cholera toxin. | the toxin of vibrio cholera causes fluid secretion from the small intestine by stimulation of adenylate cyclase and elevation of intracellular cyclic amp concentrations. the toxin is a protein composed of subunits responsible for binding to cell membranes and a subunit responsible for the activation of adenylate cyclase. the binding subunits (b) are non-covalently bonded to the active subunit (a). the latter is composed of two polypeptides a1 and a2 linked by a disulphide bridge. exposure of the ... | 1976 | 1009263 |
| spectrophotometric studies of base composition & helix-coil transition of vibrio cholerae dna. | 1976 | 1010578 | |
| effects of noxious stimulation of the skin on transmission through the spinocervical tract [proceedings]. | 1976 | 1011101 | |
| study of genetic relationships among marine species of the genera beneckea and photobacterium by means of in vitro dna/dna hybridization. | strains representative of species of the marine genera beneckea and photobacterium were used as reference standards in in vitro dna/dna competition experiments. within a given species, strains were found to be related by over 80% competition. (competition was defined as the amount of radioactive dna displaced by heterologous dna relative to the amount displaced by homologous dna.) on the basis of interspecies competition values (expressed as averages), the following groupings could be made: 1. " ... | 1976 | 1015934 |
| [inhibiting effect of cholera vibrio neuraminidase in rauscher mouse leukemia]. | a study was made of the inhibitory effect of neuraminidase of cholera vibrio in mouse rauscher leukemia. it was shown that the processing of cells of the spleen of mice suffering from leukemia with neuraminidase (in a dose of 50 units/ml and more) significantly inhibited the capacity of these cells to induce leukemia in their injection to balb/c mice. in the mentioned concentration neuraminidase injected repeatedly parenterally produced no therapeutic effect in rauscher leukemia. | 1976 | 1016719 |
| antibiotic sensitivity pattern of v. cholerae strains isolated in 1974. | 1976 | 1017856 | |
| [small outbreak of cholera in the province of orense]. | 1976 | 1019557 | |
| adjuvant immunochemotherapy of murine neoplasms. | 1976 | 1019835 | |
| synergistic lymphocyte stimulation to tumor--specific antigens after immunization with modified tumor cells. | 1976 | 1019838 | |
| serological response and serum immunoglobulin levels in volunteers immunized with cholera toxin. | 1976 | 1022695 | |
| [role of local factors of immunity with regard to cholera infection. i. localization and multiplication of vibrios in ligated small intestine loops of non-immunized rabbits and newborn animals]. | 1976 | 1023438 | |
| [improvement in the method of obtaining monospecific sera to soluble antigens]. | 1976 | 1023445 | |
| sensitivity of vibrio group of organisms to minocycline--a new tetracycline derivative. | 1976 | 1024085 | |
| [long-term survival of e1 tor cholera vibrios in naturally contaminated sewage]. | el tor cholera vibrios of ogava serological type were revealed in the sewage of the locomotive shed for 15 months. in experiment with an oil catcher in naturally infected sewage el tor vibrios survived 36 days, in storage of this sewage at the laboratory--39 days, in the artificially infected sewage of a settlement and of a milk plant--2 and 11 days, respectively, in the oil and disel fuel--14 months. consequently, el tor vibrio can survive in the sewage with a high oil product content for a lon ... | 1976 | 1024446 |
| [phage typing of cholera vibrios with different sets of phages]. | a comparative study was made of two sets of mukerjee and drozhevkina-arutyunova's bacteriophages in typing 514 strains of the el tor vibrios and 45 strains of clasic biotype. it was shown that the mukerjee or drozhevkina-arutyunova's phages could be used for the typing of cholera vibrios. the phages of the latter set prove to detect more phage types (18 against 11); they determine both the phage type and the biotype at the same time. the typing of cholera vibrios of both biotypes is possible, an ... | 1976 | 1024447 |
| [reactogenicity and immunologic effectiveness of cholerogen-anatoxin. feasibility of revaccination with cholerogen-anatoxin, determination of optimal doses and times for administration]. | the authors discussed the results of study of the titre of vibration antibodies and antitoxins one, three and five months after the vaccination of adults with an optimal vaccination dose of cholerogen toxoid (24oo bu). comparison of the immunological information obtained at the above mentioned periods showed the expediency of revaccination three months after the vaccination. immunogenicity of the three doses of the cholerogen-toxoid tested (1200, 2400, 3600 bu) displayed no statistically signifi ... | 1976 | 1024451 |
| positional distribution of fatty acids in phosphatidylethanolamine of vibrio cholerae 569 b (inaba) at different growth conditions. | 1976 | 1024965 | |
| comparative studies on the enzymes of different strains of vibrio cholerae & vibrio parahaemolyticus. | 1976 | 1024973 | |
| inhibition of adp-induced platelet aggregation as a possible test for evaluation of the enterotoxigenicity of some enterobacteria. preliminary study]. | some culture filtrates or enterotoxin preparations from enterobacteria that activate the adenylate cyclase system (vibrio cholerae, lt fraction from escherichia coli and klebsiella pneumoniae, shigella dysenteriae type 1) exibit an inhibiting effect on adp-induced platelet aggregation, while other enterotoxin preparations not effective on adenylate cyclase system, don't interfere with this model. the a. propose the platelet aggregation as cellular assay to detect enterotoxin fractions effective ... | 1976 | 1028645 |
| humoral reponse to neuraminidase-treated tumor cells. | l1210 leukemia cells grew progressively and caused tumor deaths in all recipient mice. however, when these cells had been treated with vibrio cholerae neuraminidase (vcn) prior to injection, tumor deaths did not occur. both untreated and vcn-treated l1210 cells elicited a humoral response, as manifested by an increasing percent of cells in the spleen and peritoneal cavity, with various types of membrane-associated immunoglobulins. progressive tumor growth was associated with a large percent of p ... | 1976 | 1032896 |
| [clinical and therapeutic study of cholera in dahomey. (133 clinical cases)]. | 1976 | 1036472 | |
| interactions of choleragenoid and gm1 ganglioside with enterotoxins of vibrio cholerae and escherichia coli in cultured adrenal cells. | the heat-labile enterotoxins of vibrio cholerae and escherichia coli induce morphologic changes and steroidogenesis in clonal lines of adrenal tumor cells in tissue culture; these effects are preventable by prior incubation of either toxin with gm1 ganglioside (galactosyl-n-acetylgalactosaminyl [sialosyl] lactosyl ceramide; ggnslc) but are not preventable by prior incubation of adrenal cells with choleragenoid. choleragenoid, however, is capable of interfering with the ability of gm1 ganglioside ... | 1976 | 768385 |
| isolation and properties of heat-labile enterotoxin(s) from enterotoxigenic escherichia coli. | various techniques have been applied to the detection of skin reactivity associated with heat-labile escherichia coli enterotoxin in fermenter-grown cultures of enterotoxigenic strains in syncase medium and in trypticase soy broth. isolated products that were homogeneous, as determined by disc electrophoresis and sodium dodecyl sulfate gel electrophoresis, differed immunologically and in physicochemical characteristics depending on the strain and medium used, even though the products had similar ... | 1976 | 768386 |
| purification of heat-labile enterotoxin from escherichia coli o78:h11 by affinity chromatography with antserum to vibrio cholerae toxin. | concentrated culture filtrate of escherichia coli o78:h11, strain h10407, was applied to an affinity column prepared with igg antibodies to the toxin of vibrio cholerae. elution of the retained material with 3 m kcns yielded a nonenterotoxic protein that precipitated with antiserum to v. cholerae toxin and had three major protein components on sodium dodecyl sulfate gels. after treatment with 2-mercaptoethanol, two protein components were observed. elution of the affinity column with 5 m guanidi ... | 1976 | 768387 |
| activation of heat-labile escherichia coli enterotoxin by trypsin. | trypsin-treated, cell-free filtrates derived from enterotoxigenic escherichia coli, strain h197 (o78:h11), exhibited a fourfold or greater increase in heat-labile vascular permeability factor activity and a 10-fold or greater increase in the ability to stimulate secretion of growth hormone by cultured rat pituitary cells. in contrast, trypsin-treated filtrates were not different from untreated filtrates in their ability to elicit a secretory response in ligated rabbit intestinal loops. however, ... | 1976 | 768388 |
| radiation therapy and immunotherapy: the value of immunotherapy in the control of local and regional cancer. | 1976 | 769945 | |
| [cholera]. | 1976 | 772354 | |
| [so-called mucoid forms of vibrio cholerae]. | the authors present materials indicating that examination of nonagglutinating vibrios in the agglutination reaction with cholera sera after boiling, according to sakazaki recommendation (1971), did not provide recognition of mucoid forms of cholera vibrio and its differentiation from cholera-like vibrios of other serological groups. it is suggested that one should take into consideration morphological peculiarities of the culture, stability of agglutination with cholera o-serum and the results o ... | 1976 | 773060 |
| cellular events involved in the true primary immune response of splenocytes in vitro. | an antibody response showing characteristics of a "true" primary response was readily induced in vitro with splenocytes from normal non-immunized animals cultured with strongly immunogenic vibrio cholerae somatic antigens. prior studies have shown that the response to vibrios in intact animals appeared to be a true primary response since no pre-existing antibody forming cells were present in non-immunized animals and the antigen induced response, following a lag of two days, resulted thereafter ... | 1976 | 773113 |
| [use of media with antibiotics for isolating resistant forms of the cholera vibrios]. | higher stability of resistance to tetracycline in the polyresistant strain of v. eltor under conditions of macroorganism as compared to nutrient media was found experimentally. to increase isolation of the resistant forms of the cholera vibrio it was recommended to use agar with tetracycline or other antibiotics depending on the particular case in addition to the routine media. | 1976 | 776072 |
| vibrio cholerae infection and immunity in mice. | lyophilized cultures of v. cholerae 569b slowly lose their virulence for neonatal and adult mice during long term storage. following a single passage in orally infected 6-day old mice, a highly virulent strain (designated 569b/mp) was isolated. this organism causes rapidly fatal intestinal infections in 6-day old mice; large numbers can be isolated in pure culture from the intestinal fluid. freezing and storage at -60 degrees of dead animal provides a simple means of maintaining the high virulen ... | 1976 | 779744 |
| adhesive properties of vibrio cholerae: nature of the interaction with intact mucosal surfaces. | two companion papers in this series have characterized the interaction between vibrio cholerae and the surfaces of eukaryotic cells. the present paper reports studies of the association between vibrios or salmonella enteritidis and intact slices of intestinal tissue. a significant number of differences were noted in the characteristics of bacterial adhesion in these systems. the results are interpreted to indicate the presence of at least two receptors for vibrio adhesion on the mucosal surface ... | 1976 | 780274 |
| challenge of dogs with live enterotoxigenic escherichia coli and effects of repeated challenges on fluid secretion in jejunal thiry-vella loops. | dogs were evaluated as experimental models for the study of diarrheal disease produced by enterotoxigenic escherichia coli. although a suitable whole model for orogastric bacterial challenge could not be developed, chronic jejunal thiry-vella loops were used to study the secretory effects of multiple jejunal challenges with enterotoxin of either vibrio cholerae or e. coli. the heat-stable and heat-labile e. coli enterotoxins could be differentiated clearly in this model. sequential weekly challe ... | 1976 | 781145 |
| efficacy of a live oral cholera vaccine in human volunteers. | a live attenuated oral cholera vaccine has been given to volunteers with complete safety. the vaccine strain appeared to multiply significantly in intestinal contents and to result in appreciable vibriocidal antibody response. subsequent challenge with virulent inaba vibrios demonstrated substantial protection from clinical illness and from vibrio shedding. the possibility of reversion to toxin production is discussed. | 1976 | 782960 |
| immunological probes into the mechanism of cholera toxin action. | the use of antibodies to specific cell surface proteins or to ligands which interact with cell surface receptors is a powerful tool for analyzing the properties of membrane proteins and the consequences of specific cell surface ligand-receptor interactions. two central observations concerning membrane structure and function, - the diffusibility of membrane proteins (1) and ligand-triggered modulation of specific receptors (2), have derived from the use of antibodies to analyze the properties of ... | 1976 | 786864 |
| differential biological characteristics of vibrio cholerae and escherichia coli enterotoxins. | 1976 | 786870 | |
| isolation of skin permeability factors from culture filtrates of salmonella typhimurium. | engerotoxins isolated from vibrio cholerae and toxigenic escherichia coli cause permeability alterations in rabbit skin. firm induration and erythema are observed within 18 to 24 h, and visualization of the reaction may be enhanced by intravenous injection of pontamine sky blue dye. two skin permeability factors (pf) have been found in culture filtrates of salmonella typhimurium. a rapid acting factor, produced optimally in brain heart infusion broth at 37 degrees c by numerous salmonella specie ... | 1976 | 786890 |
| effect of enterotoxins of vibrio cholerae, escherichia coli, and shigella dysenteriae type 1 on fluid and electrolyte transport in the colon. | experiments were designed to determine whether the enterotoxins of vibrio cholerae, escherichia coli, and shigella dysenteriae type 1 alter the movement of fluid and electrolytes in the rat cecum. net secretion of water and sodium were observed after incubation of 1.67 x10(-5) mug of purified cholera toxin (choleragen)/ml for 18 hr or of 50 mug/ml for 3 hr. the effect of choleragen on cecal transport of water and electrolytes was related to the dose. in addition, choleragen increased cecal mucos ... | 1976 | 787442 |
| blast crisis of chronic myeloid leukaemia (cml). ii. cell surface marker analysis of "lymphoid" and myeloid cases. | fourteen cases of philadelphia chromosome (ph1) positive chronic myeloid leukaemia in blast transformation have been investigated using cell surface markers. morphologically eight cases were lymphoid and the remainder myeloid in appearance. all cases were negative with surface markers for thymocytes and t and b lymphocytes. five of the lymphoid cases reacted with an antiserum specific for acute lymphoid leukaemia )all) of non-t non-b type and were also weakly reactive with a lymphocyte reactive ... | 1976 | 788772 |
| correlations between humoral immunity and successful chemotherapy-immunotherapy. | experiments were designed to evaluate the characteristics of the humoral immune response induced by active immunotherapy, both specific (neuraminidase-treated tumore cells) and nonspecific (bacillus calmette-guérin organisms), in the l1210-c57bl/6 x dba/2f tumor-host system. tumor burden was minimized with chemotherapy (1,3-bis-(2-chloroethyl)-1-nitrosourea) prior to immunotherapy. a marked increase in the concentration of serum immunoglobulins (immunoglobulin m, immunoglobulin g, and immunoglob ... | 1976 | 788896 |
| neutralizing antibodies against escherichia coli and vibrio cholerae enterotoxins in human milk from a developing country. | by means of the adrenal cell assay and the rabbit small-bowel loop technique enterotoxin-neutralizing antibodies were demonstrated in all investigated milk samples from severely undernourished pakistani women but, with a single exception, not in milk from swedish mothers. the antibodies belonged to the iga and igg classes as observed in an enzyme-linked immunosorbent assay, which also revealed secretory component on specific enterotoxin antibodies, showing that the iga antibodies were primarily ... | 1976 | 790543 |
| [effect of cholera vibrio and escherichia coli enterotoxins on human thrombocyte aggregation induced by adenosine diphosphate]. | 1976 | 790824 | |
| effects of doxycycline in actively purging cholera patients: a double-blind clinical trial. | in 51 actively purging cholera patients the efficacy of doxycycline, a long-acting tetracycline, was compared with a placebo and tetracycline hydrochloride. seventeen patients who were given doxycycline at the recommended dose of 2 mg/kg at the beginning of the study, at 12 h, and at the repeated dose once daily purged a mean volume of 5.1 liters of stool and received an average of 5.7 liters of intravenous fluid. nineteen patients receiving the placebo purged 10.1 liters of stool and received 9 ... | 1976 | 791107 |
| differential expression of cell surface binding sites for cholera toxin in acute and chronic leukaemias. | binding of purified cholera toxin to cell surface receptors has been visualized by an indirect immunofluorescence procedure. normal nucleated cells from blood, bone marrow and lymphoid tissues, express these receptors with the possible exception of erythroid precursors. cells from patients with chronic lymphoid or myeloid leukaemias have a normal receptor expression but acute leukaemic cells showed a marked deficiency in cholera toxin binding. insertion of purified gm ganglioside into membranes ... | 1976 | 791345 |
| the problem of bacterial diarrhoea. | the reported incidence of "pathogenic" bacteria, as judged by serotype, in the stools of children with acute diarrhoea has varied from 4 to 33% over the last twenty years. techniques such as tissue culture provide a means for detecting enterotoxin-producing strains of bacteria, strains which often do not possess "pathogenic" serotypes. "pathogenicity" requires redefinition, and the aetiological importance of bacteria in diarrhoea is probably considerably greater than previous reports have indica ... | 1976 | 791597 |
| taking science where the diarrhoea is. | with attack rates exceeding two episodes per year in the young diarrhoea with attendant dehydration is by far the major single killer in the developing world. an invariable accompaniment of the more insidious and chronic protein-energy malnutrition (pem), diarrhoea is itself an acute form of malnutrition: fluid-electrolyte malnutrition (fem). scientific attention to fem has focused heavily on mechanisms of pathogenesis and disordered physiology, often to the neglect of preventive and effective c ... | 1976 | 791598 |
| the nature and action of cholera toxin. | cholera diarrhoea is due to the action of a toxin that acts on all animal cells by stimulating the enzyme adenylate cyclase, which catalyses the production oc cyclic amp from atp. in intestinal brush border cells raised cyclic amp levels result in increased secretion of chloride ions, leading to fluid accumulation in the gut. escherichia coli produces a similar toxin. the receptor for cholera toxin on the cell membrane appears to be a complex containing the ganglioside ggnslc (or gm1). cholera t ... | 1976 | 791600 |
| [method for the quantitative determination of penicillin acylase activity by the formation of phenylacetic acid]. | the quantitative method for determination of penicillinacylase activity is described. the method is based on detection of phenylacetic acid (paa) formed during hydrolysis of benzylpenicillin. paa is extracted with toluol and nitrated with potassium nitrate solution in concentrated sulphuric acid followed by reduction of nitrophenylacetic acid into aminophenylacetic acid with zinc powder. aminophenylacetic acid interacts with p-dimethylaminobenzaldehyde in acid medium forming a coloured compound ... | 1976 | 793510 |
| [determination of the activity of vibrio cholerae antibodies in human and animal sera by means of the bactericidal test]. | 1976 | 794601 | |
| [enterobacterial enterotoxins]. | 1976 | 795228 | |
| analysis of human leukaemic cells using cell surface binding probes and the fluorescence activated cell sorter. | cell surface binding fluorescent ligands have been used to distinguish between different types of leukaemic cells and between leukaemic cells and their presumed normal counterparts or progenitors. binding of these probes was evaluated using the fluorescence activated cell sorter (facs) which provides both rapid, objective and quantitative recording of fluorescent signals from individual cells plus physical separation of cells of particular interest. binding sites for cholera toxin (monosialogang ... | 1976 | 795743 |
| studies on the effect of phytagglutinins on some members of enterobacteriaceae. | extracts of twenty seeds were tested against twenty six organisms belonging to klebsiella, proteus, salmonella, shigella and cholera species. extracts of eleven seeds showed agglutinating activity against twenty four various organism. s. typhi and kl. aerogenes did not react against any lectin. on the basis of the results it was possible to differentiate various shigella and salmonella organisms by various seed extracts. different isolates of cholera organisms of same serotype and phage type sho ... | 1976 | 796502 |
| [practical interest of a micromethod for neuraminidase treatment of lymphocytes in transplantation immunology (author's transl)]. | vibrio cholerae neuraminidase treatment increases the cell sensitivity to complement and antibodies cytotoxic action. this property can be applied to the microlymphocytotoxicity technic for antibodies study in dialysed and kidney transplanted patients and for pretransplantation cross-matches. the enzymatic treatment usually employed needs a great deal of lymphocytes submitted in a second step to antibodies cytotoxic action. but this method appeared difficult to be routinely applied. we developed ... | 1976 | 796790 |
| [possible use of the platelet as the cell system for the control of enteropathogenicity of e. coli and of other enterobacteria. preliminary approach]. | after an introduction concerning the assays employed in vitro and in vivo to determine enterotoxigenic activity of e. coli, the a. suggests a new in vitro model as a preliminary approach based upon the influence of lt enterotoxin of escherichia coli on platelet aggregation (inhibitory effect) as expression of stimulation of adenylatecyclase activity evoked by this fraction and eventually other cholera-like fractions released from enterobacteria. | 1976 | 797394 |
| adhesion of cholera phage to glass surfaces at high inactivation temperatures. | decimal dilutions of cholera phage heated in test tubes at the temperature range of 65 degrees to 70 degrees showed an erratic behaviour in that the residual counts had no relationship to the quantity of phage originally present in the tubes. if the contents of the heated tubes were decanted off and the empty tubes washed repeatedly with broth, the recovery of phage from successive washings of the tubes was much higher than what would be expected on the basis of the simple dilution effect of was ... | 1976 | 798584 |
| comparative study of reactions and serological response to cholera vaccines in a controlled field trial conducted in the ussr. | this article presents the results of a comparative study of the reactogenicity and the serological response induced by a number of cholera vaccines. conducted in the ussr on 998 adults aged 18 years and over, the study covered whole-cell heat-killed and formalin-inactivated cholera vaccines, whole-cell heat-killed el tor vaccine, and a new partially purified toxoid preparation proposed for the immunoprophylaxis of cholera-all administered by hypodermic syringe or jet injector. the most marked re ... | 1976 | 798636 |
| vibrio parahaemolyticus: aetiological agent of food poisoning. | after mentioning the principal morphological, cultural, physiological, biochemical, enzyme and serological characteristics of vibrio parahaemolyticus (a facultative halophilic bacterium and the aetiological agent of food poisoning caused by the consumption of fish products) a description is given of its ecological aspects, geographical distribution, and potential enteropathogenicity for man, with general information on its isolation and identification. | 1976 | 798718 |
| [manifestation of intestinal immunity to vibrio cholerae]. | 1976 | 799289 | |
| [microagglutination reaction with labeled cholera vibrios]. | 1976 | 799370 | |
| [cell envelopes and lipopolysaccharides of gram-negative bacteria (author's transl)]. | 1976 | 800276 | |
| [future perspective in the use of disinfectants]. | the effects of different combinations of quaternary ammonium compounds (sanitized, sanigard) on faecal and soil organisms demonstrate the usefulness of these products (dilution 1/100,000). a contact time of 30 seconds to 4 minutes is sufficient for bacteriocidal activity. materials treated with these compounds retain their bacteriocidal activity after 10 washes with boiling water and for over a month with normal usage. the disinfection of a door handle contaminated with vibrio cholerae or salmon ... | 1976 | 801260 |
| immunological studies in murine osteosarcoma. immunogenicity, growth kinetics, and immunotherapy. | a transplantable murine osteosarcoma is described. following transplantation into a syngeneic mouse the tumor grows rapidly and kills the mouse with pulmonary metastases simulating human osteosarcoma. a cell-mediated antibody response is evoked in the host mouse as demonstrated by in vivo and in vitro tests. the number of pulmonary metastases may be decreased with adjunctive immunotherapy following excision of the primary tumor. immunotherapeutic materials include bcg and isologous cells treated ... | 1976 | 1063129 |
| immunotherapy of human leukemia with neuraminidase-modified cells. | 1976 | 1063908 | |
| comparative study of alkaline phosphatase activity in lymphocytes, mitogen-induced blasts, lymphoblastoid cell lines, acute myeloid leukemia, and chronic lymphatic leukemia cells. | alkaline phosphatase [orthophosphoricmonoester phosphohydrolase (alkaline ph optimum), ec 3.1.3.1] purified from a burkitt lymphoma cell line (daudi) and moloney-virus-induced murine leukemia (yac) showed unique catalytic properties in substrate specificity and inhibition by cysteamine-s-phosphate. it migrated on polyacrylamide gel electrophoresis in a single activity band. alkaline phosphatase with similar properties was found in several human lymphoblastoid cell lines, in chronic lymphatic leu ... | 1976 | 1064015 |
| elucidation of hydrocarbon structure in an enzyme-catalyzed benzo[a]pyrene-poly (g) covalent complex. | the carcinogen, benzo[a]pyrene, was covalently attached to poly (g) by liver microsomes from rats pretreated with 3-methylcholanthrene. the complex was hydrolyzed with enzymes or base and products were isolated by sephadex chromatography. absorbance and fluorescence spectra of the products fit that of red-shifted pyrene aromatic system and suggest that metabolism has occurred at the 7-, 8-, 9-, and 10-positions of the hydrocarbon. benzanthracene or chrysene fluorescence were not observed in thes ... | 1976 | 1064016 |
| immune markers in adult acute leukemia. | three cases of adult acute leukemia were examined for immunologic markers. incubation of leukemic cells with anti-d (igg)-coated erythrocytes was used for the identification of monocytes; sheep erythrocytes were employed for t-cells and fluorescein-conjugated anti-mixed immunoglobulins for b-cells. it was shown that igg receptors (monocytes) were present on immature cells with predominant monocytic components; t- and b-cell markers were absent in the mono- and myelocytic leukemias. | 1976 | 1065217 |
| inhibition of calcium phosphate precipitation by human salivary secretions. | 1976 | 1065265 | |
| [induction of phagocytosis by immunotherapy against leukemic leukocytoblasts]. | since 1975, the authors have been working in immunochemotherapy in acute myeloblastic leukemic adult patients. autologous leukemic leucocytoblasts have been cultured with vibrio cholerae neuraminidase (nvc). thereafter, neoplastic cells were radiated with cobalt bomb and frozen to--70degreesc and afterwards 5 x 10(6) of these leucoblasts were intradermally injected to each patients every eight days. all patients received between six to ten injections. so far, ten such patients have been followed ... | 1976 | 1066088 |
| immune responses in cholera vaccinated persons. | 1976 | 1066435 | |
| choleragen-mediated release of trapped glucose from liposomes containing ganglioside gm1. | 125i-labeled choleragen was bound to liposomes containing galactosyl-n-acetylgalactosaminyl-(n-acetylneuraminyl)-galactosylglucosylceramide (gm1), but not in large amounts to ganglioside-free liposomes nor to those containing n-acetylneuraminylgalactosylglucosylceramide (gm3), n-acetylgalactosaminyl-(n-acetylneuraminyl)-galactosylglucosylceramide (gm2), or n-acetylneuraminylgalactosyl-n-acetylgalactosaminyl-(n-acetylneuraminyl)-galactosylglucosylceramide (gd1a). choleragen released trapped gluco ... | 1976 | 1068459 |
| therapeutic effectiveness of neuraminidase-treated tumor cells as an immunogen in man and experimental animals with leukemia. | the immunogenicity of leukemia l1210 in dba/2 ha and 6c3hed lymphosarcoma tumor cells in c3h/f mice was significantly increased after treatment with v. cholerae neuraminidase. dba/2 ha and c3h/f mice repeatedly immunized with neuraminidase-treated tumor cells rejected subsequent challenge of 10(7) or 10(6) untreated tumor cells, respectively. based on the 51cr microcytotoxicity assay, both strains of mice showed strong complement-dependent antibody titers and cell-mediated immunity. sera and spl ... | 1976 | 1069551 |
| adjuvant immuno- and/or chemotherapy with neuraminidase-treated autogenous tumor vaccine and bacillus calmette-guérin for head and neck cancers. | 1976 | 1069553 | |
| inter-relationship between autoregulation of glomerular filtration rate, tubuloglomerular feedback and juxtaglomerular renin activity in normotensive and hypertensive rats. | 1. reduction of renal perfusion pressure from 133 mmhg to 117 mmhg in control rats did not induce a significant change of kidney glomerular filtration rate (gfr) or nephron gfr determined in distal tubules. in contrast, nephron gfr measured in proximal tubular segments (ngfr-p) fell significantly. 2. qualitatively the same response of filtration rate to changes of arterial blood pressure was found in the chronically clipped kidneys of goldblatt hypertensive rats after acute removal of the clip. ... | 1976 | 1071583 |
| nutritional status: a determinant of severity of diarrhea in patients with cholera. | the severity of diarrhea and nutritional status were measured in a prospective study of 97 patients hospitalized with cholera in dacca, bangladesh. ninety-five percent of both adults and children were below their respective medians in weight as related to height; greater than 15% of each group showed second-degree protein-calorie malnutrition. duration of diarrhea, but no volume of stool per hour, was prolonged by 30%-70% in those adults and children suffering from more severe malnutrition. the ... | 1976 | 820813 |
| lectin and cholera toxin binding to guinea pig tumor (104c1) cell surfaces before and after glycosphingolipid incorporation. | 1976 | 822849 | |
| preparation of a purified antigenic cholera toxoid. | purified cholera enterotoxin was prepared by methods described by finkelstein and lo spalluto (1970). this toxin was detoxified by treatment with heat and formaldehyde. heating cholera toxin at 60 c for 25 min resulted in the formation of a polymer named procholeragenoid by finkelstein et al. (1971). the weak toxic activity of this product was removed by treatment with formalin. no residual toxicity could be demonstrated in formalinized procholeragenoid by the rabbit ileal loop assay and the hig ... | 1976 | 823107 |
| development of a purified cholera toxoid. iii. refinements in purification of toxin and methods for the determination of residual somatic antigen. | the addition of an ultrafiltration step to the purification procedures previously described for cholera toxin (rappaport et al., (1974) permitted the preparation of highly purified antigenic toxoids essentially free of somatic antigen(s). the purity of such toxoids is established: (i) by the absence of more than about one part limulus amebocyte lysate (lal)-positive endotoxin per 10(5) parts toxoid and (ii) by the inability of the toxoids to elicit a significant rise in rabbit vibriocidal antib ... | 1976 | 823117 |
| [role of l-forms of bacteria and mycoplasma in the etiology and pathogenesis of several acute and chronic diseases]. | 1976 | 823732 | |
| [cholera]. | 1976 | 824378 | |
| an evaluation of commercial thiosulphate citrate bile salt sucrose agar (tcbs). | 1976 | 825501 | |
| malnutrition and susceptibility to infection with vibrio cholerae in vervet monkeys (cercopithecus aethiops). 1. induction of protein, b-vitamin complex and calorie malnutrition. | five groups of eight vervet monkeys (cercopithecus aethiops) were fed regular monkey chow, low protein (lp), low b-vitamin complex (lbv), low protein - low b-vitamin complex (lb-lbv), or protein - low calorie (lp-lc) diet, respectively, for eight weeks. hematocrit values, hemoglobin, bun, total protein, albumin, riboflavin and body weight decreased, the ratio of dispensable/indispensable amino acids increased in all animals receiving a deficient diet. total globulin, igg and igm remained unchang ... | 1976 | 826634 |
| malnutrition and susceptibility to infection with vibrio cholerae in vervet monkeys (cercopithecus aethiops). 2. response of vervet monkeys on protein, b-vitamin complex and calorie-deficient diets to infection. | five groups of vervet monkeys (cercopithecus aethiops) were fed regular monkey chow, low protein (lp), low b-vitamin complex (lbv), lp-lbv, or low protein- low calorie (lp-lc) diets, respectively. after eight weeks the animals were infected with cholera vibrios. igg, iga and igm increased in the sera of all animals after the challenge but the avidity of the immunoglobulins was lower in vervets on lp diets. diarrhea and excretion of vibrios lasted longer in animals consuming less protein. | 1976 | 828672 |
| [the cholera syndrome - pathogenesis and pathogens (author's transl)]. | the same pathomechanism underlies both classical cholera and the enteritides due to nonagglutinating (nag) vibrios and a few types of e. coli. a heat-labile enterotoxin activates the adenocyclase. this stimulates the cells of the small intestine to secrete ions and water, the immune reaction in the intestine is retarded and the production of gastric juice is reduced. neuraminidase potentiates these reactions by opening additional enterotoxin receptors. endotoxin is a further pathogenetic factor. ... | 1976 | 814420 |
| escherichia coli enterotoxin: purification, partial characterization, and immunological observations. | enterotoxin, a diarrhea-inducing protein elaborated by pathogenic escherichia coli strains, was isolated from the supernate of fermenter cultures of e. coli strain p263, a porcine enteropathogen. purification involved chromatography and preparative isotachophoresis. the resulting product appeared to be pure according to immunoelectrophoretic, disc electrophoretic, ultracentrifugal, and immunologic criteria. the enterotoxin had an apparent molecular weight of 102,000 daltons, and its isoelectric ... | 1976 | 815447 |
| chemistry of cholera toxin: the subunit structure. | the toxin of vibrio cholerae was separated into two subunits by gel filtration on sephadex g-75 in 5% formic acid. the subunits were designated a and b. amino acid analysis indicated that subunit b corresponded to choleragenoid. renatured subunit b was found to be antigenically identical to the whole molecule, whereas renatured subunit a was not. on reduction and s-carboxymethylation with [2-14c] iodoacetate in 8 m urea, subunit a separated into two polypeptides of unequal sizes, a1 and a2, with ... | 1976 | 815448 |
| size and structure of the cholera toxin molecule and its subunits. | cholera toxin (choleragen) dissociated into two types of subunit with molecular weights estimated to be 28,000 daltons (a) and 11,000 daltons (b); this dissociation was effected by gel filtration at acid ph with or without urea. subunit a could be separated into two fragments, a1 (23,000 daltons) and a2 (about 2,500 daltons), after reduction and alkylation. choleragenoid (68,000 daltons) appeared to be a polymerized form of subunit b. a-specific antigen was found in choleragen as well as in a1 a ... | 1976 | 815449 |
| the subunits of cholera toxin: structure, stoichiometry, and function. | the toxin of vibrio cholerae dissociates into subunit a and an aggregate of subunit b (choleragenoid); the dissociation is rapid under denaturing conditions and slow at neutral ph. subunit a has a molecular weight of 27,000 daltons (measured by sedimentation equilibrium or gel chromatography) and has two polypeptide chains (mol wt, approximately 22,000 and 5,000 daltons) joined by disulfide bonds. the molecular weight of subunit b in 6 m guanidine hydrochloride is 14,000 daltons when determined ... | 1976 | 815450 |
| ampicillin-resistant hemophilus sp. | 1976 | 1082793 | |
| [influence of substances that change the serotonin level of the brain on the analgesic effect of promedol in rats]. | in tests with determination of pain reaction in rats registered by their squeak and arising in reponse to a single electric irritation of the tail evidence was obtained to the effect that substances both lowering the serotonin content in the brain (p-chlorphenylalanine, reserpine) and raising it (5-oxytryptophan, pargilin) cause a fall of the threshold, viz. 5-oxytryptophan and pargilin display antagonism to the analgetic effect of trimeperidine hydrochloride, whereas p-chlorphenylalanine, and i ... | 1976 | 1082823 |
| epithelial regeneration of transposed intestine after high doses of x-irradiation. | the regeneration capacities of normal and transposed small bowel epithelium were compared in rats after applying high doses of x-irradiation. it has been shown that the potency of the mucosa to regenerate is much higher than assumed and that the mucosa can regenerate after single doses varying from 2000-5000 r. even in the villus epithelium and in flat epithelium covering infiltrates of the lamina propria cells survive, which are still able to resume proliferative activity several days after irr ... | 1976 | 1083838 |
| photobiological activity of furazolidone. | 1976 | 1086295 | |
| a streptomycin-sensitive revertant of a streptomycin-dependent strain of vibrio cholerae. | a study was made of a streptomycin-sensitive revertant of a streptomycin-dependent strain of v. cholerae. the revertant, designated as strain kb599, appears to be avirulent in animal experimental models. however, no evidence of multiplication was found in infant mice. | 1976 | 1086742 |
| intestinal immunization with soluble bacterial antigens: the example of cholera toxoid. | the studies described are aimed at a better understanding of the intestinal immunological system and its role in protection against enteric infection. the cellular kinetics of the intestinal immune response to cholera toxoid were studied in rats and the protection afforded by toxoid immunization was studied in dogs. memory was demonstrated in the gut immune system. plasma cells containing iga antitoxin appeared in large numbers in gut lamina propria when intraduodenal boosting followed either in ... | 1976 | 1086763 |
| membrane expression of thy-1,2 and gm1 ganglioside on differentiating t lymphocytes. | c3h mouse bone marrow cells were separated by discontinuous bsa gradient centrifugation. marrow cells from the 17 to 19%, 19 to 21% to 23%, 23 to 25%, 25 to 27% interfaces and the cell pellet were treated with thymic factor (tf) or with vibrio cholerae neuraminidase (vcn), followed by anti-thy-1,2 and anti-gm1 ganglioside antisera. antigens determined by anti-thy-1.2 or anti-gm1 were expressed either with tf or vcn within a 30-min incubation. cells expressing these antigens after vcn or tf treat ... | 1976 | 1086862 |
| surface molecules of cultured human lymphoid cells. | cell surface molecules of cultured human lymphoid cells were selectively labeled by lactoperoxidase-catalyzed iodination and examined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. two major iodinated species with apparent mol. wts. of 27 000 and 35 000 daltons were detected on autoradiographs of the labeled proteins of human lymphoid cell lines believed to be of thymus-independent (b) cell origin. neither molecule was detected on putative thymus-dependent (t) l ... | 1976 | 1087235 |