Publications

comparison between enzyme-linked immunosorbent and i.f. assays in the serological diagnosis of hiv infections.	a rapid, sensitive indirect immunofluorescence assay based on the use of acetone-fixed virus producing molt t4/lav cells was adapted for the detection of anti-hiv antibodies. at the same time, 1486 serum samples, collected by the aids surveillance and study centre of modena, were tested by elisa and ifa: the percentage of agreement of both assays was of 99.66%. such datum suggested that ifa could be used as a serological assay for screening and confirmatory purposes.	1988	3280951
b lymphocyte function in patients with rheumatoid arthritis: impact of regulatory t lymphocytes and macrophages--modulation by antirheumatic drugs.	the present work analyses b lymphocyte functions in vitro in patients with rheumatoid arthritis (ra). the impact of gold salts and penicillamine on human b lymphocyte function in vitro is discussed. synovial fluid monocytes/macrophages increased both the polyclonally induced and the antigen-induced blood lymphocyte proliferation and increased the numbers of immunoglobulin-secreting blood b lymphocytes generated by pokeweed mitogen (pwm), a t cell-dependent polyclonal activator. the lymphostimula ...	1988	3282810
escherichia coli mutations that prevent the action of the t4 unf/alc protein map in an rna polymerase gene.	bacteriophage t4 has the substituted base hydroxymethylcytosine in its dna and presumably shuts off host transcription by specifically blocking transcription of cytosine-containing dna. when t4 incorporates cytosine into its own dna, the shutoff mechanism is directed back at t4, blocking its late gene expression and phage production. mutations which permit t4 multiplication with cytosine dna should be in genes required for host shutoff. the only such mutations characterized thus far have been in ...	1988	3282983
an in vitro assay for frameshift mutations: hotspots for deletions of 1 bp by klenow-fragment polymerase share a consensus dna sequence.	the fidelity of in vitro dna synthesis catalyzed by the large fragment of dna polymerase i was examined. the templates, specifically designed to detect shifts to the +1 or to the -1 reading frame, are composites of m13mp8 and bacteriophage t4 riib dna and were designed to assist in the identification of the types of frameshifts that are the specific consequence of dna polymerization errors. in vitro polymerization by the klenow fragment produced only deletions, rather than the mixture of duplica ...	1988	3282984
[a case of subacute thyroiditis associated with acute hepatitis].	we report one case of subacute thyroiditis associated with acute hepatitis, which is histopathologically diagnosed. a 43-year-old woman visited our hospital with chief complaints of fever, sore throat and anterior neck pain. thyroid gland was found to be swollen and tender. laboratory findings gave high esr and positive test for crp. high values of t3, t4 and rt3u indicated that the patient had hyperthyroidism. however no autoantibodies against thyroglobulin and microsome were found. high activi ...	1988	3283015
resting metabolic rate and postprandial thermogenesis in highly trained and untrained males.	the purpose was to compare the resting metabolic rate (rmr) and thermic effect of a meal (tem) in exercise trained and untrained individuals. tem was measured for 180 min and blood samples were drawn for determination of plasma insulin, glucose, triiodothyronine (t3) and thyroxine (t4). results indicated that highly trained subjects demonstrated a higher rmr when expressed in kilocalories per minute and per kilogram fat-free weight (ffw) than do untrained subjects. tem was lower in trained (55.8 ...	1988	3284328
effect of ph on the base-mispairing properties of 5-bromouracil during dna synthesis.	we have utilized an electrophoretic assay of misincorporation to investigate the possibility that ionization of 5-bromouracil (bu) may play a role in its mispairing during dna synthesis in vitro. we examined the effects of increasing ph on the relative rates of formation of bu.g and t.g mispairs during chain elongation catalyzed by various dna polymerases. for the klenow fragment of escherichia coli dna polymerase i, increasing ph facilitated bu.g mispair formation (relative to t.g mispairing) w ...	1988	3284589
glucose tolerance and pancreatic hormones in thyroidectomized and thyroid hormone-injected cockerels.	the effect of surgical thyroidectomy and of t4/t3 injections on basal and glucose-induced concentrations of plasma insulin and glucagon has been investigated in 20-week-old domestic chickens. birds injected daily (im) for 2 weeks with t4/t3 (50 micrograms/day) had marginally lower fasting glucose concentrations whereas thyroidectomy had no effect. glucose tolerance to an intravenous injection of glucose (0.5 g/kg) was impaired in t4/t3 injected animals although the peak hyperglycemia was identic ...	1988	3284781
paracortical immunoregulatory subpopulations in lymph nodes from homosexual men with persistent generalized lymphadenopathy.	homosexual patients with persistent generalized lymphadenopathy usually show an abnormal expansion of paracortical t8+ lymphocytes relative to t4+ cells in lymph nodes with reactive follicular hyperplasia (rfh). this study was designed to characterize further the paracortical lymphocyte population in homosexual men with reactive follicular hyperplasia, because t8+ lymphocytes are antigenically and functionally heterogeneous. frozen lymph node tissue from 10 patients was evaluated. monoclonal ant ...	1988	3284806
alterations in immunologic measurements in patients experiencing early hepatic allograft rejection.	we compared lymphocyte subsets, the t4:t8 ratio, and assays of lymphocyte function in 11 recipients of hepatic allografts who showed signs of early graft rejection (less than 3 weeks after transplant) with 13 patients who had no evidence of graft rejection. both patient groups had similar values for lymphocyte subsets, lymphocyte transformation in the presence of phytohemagglutinin, and in vitro immunoglobulin synthesis prior to allografting. nonrejectors had a decline in both t3 and t4 cells on ...	1988	3285538
determination of acquired immunodeficiency syndrome (aids) after renal transplantation.	diagnosing the acquired immunodeficiency syndrome (aids) in transplant recipients can be difficult due to the patient's medication-induced immunosuppressed state. we report two renal allograft recipients who acquired hiv infection at the time of transplantation and later went on to develop multiple opportunistic infections. careful documentation of hiv antibody status of the donor and recipient, when available, the nature of immunosuppressive therapy used, the type of infections and their timing ...	1988	3285670
site-specific oligonucleotide-directed mutagenesis using t4 dna polymerase.	a simple and efficient mutagenesis procedure is described which uses both the 3'----5' exonuclease and 5'----3' polymerase activities of t4 dna polymerase. different types of mutation-deletion, insertion, and substitution-can be introduced into the dna in a single reaction. the technique uses recombinant m13 single-stranded dna and two complementary dna oligonucleotides to target and control the extent of deletions catalyzed by t4 dna polymerase. the second oligonucleotide not only directs ligat ...	1988	3286164
effect of androgen and thyroid hormones on renin-1 messenger ribonucleic acid levels in mouse submandibular gland.	the synthesis of renin and other biologically active polypeptides in the granular convoluted tubule cells of the mouse submandibular gland (smg) is regulated by androgen and thyroid hormones. in this study genetically hypothyroid (hyt/hyt) mice carrying a single renin structural gene (ren-1) were used to investigate the mechanism of hormonal action in mouse smg. treatment of female mice with 5 alpha-dihydrotestosterone (dht) and/or thyroxine (t4) enhanced renin-1 activity and increased renin-1 m ...	1988	3286326
primary structure of t4 dna polymerase. evolutionary relatedness to eucaryotic and other procaryotic dna polymerases.	bacteriophage t4 gene 43 codes for the viral dna polymerase. we report here the sequence of gene 43 and about 70 nucleotides of 5'- and 3'-flanking sequences, determined by both dna and rna sequencing. we have also purified t4 dna polymerase from t4 infected escherichia coli and from e. coli containing a gene 43 overexpression vector. a major portion of the deduced amino acid sequence has been verified by peptide mapping and sequencing of the purified dna polymerase. all these results are consis ...	1988	3286635
dna unwinding and inhibition of t4 dna ligase by anthracyclines.	the ability to alter dna tertiary structure of ten anthracycline derivatives whose antitumor potency is known was studied by an assay that makes use of nicked circular dna and bacteriophage t4 dna ligase. this assay allows the detection of tertiary structure alterations caused by dna binding of both intercalating and non-intercalating drugs. the determination of these events can be obtained at different temperatures in the range of activity of dna ligase. the results indicate that anthracyclines ...	1988	3287337
characterization of canine triiodothyronine (t3) autoantibodies and their effect on total t3 in canine serum.	a study of 3,5,3'-l-triiodothyronine autoantibody (t3 aa) in 18 dogs revealed an average apparent affinity constant for t3 of 2.24 +/- 1.78 x 10(10) m-1, an average t3 binding capacity of 639.3 +/- 666.5 ng/dl and a low thyroxine (t4) cross-reactivity (less than 1%) in all samples tested. a valid radioimmunoassay (ria) procedure which involved heat treatment of samples for 1 hr at 70 degrees c and assay on sephadex minicolumns was developed for measuring t3 in the presence of t3 aa. total t3 was ...	1988	3287386
ergothioneine, histidine, and two naturally occurring histidine dipeptides as radioprotectors against gamma-irradiation inactivation of bacteriophages t4 and p22.	bacteriophages p22, t4+, and t4os (osmotic shock-resistant mutant with altered capsids) were diluted in 0.85% nacl and exposed to gamma irradiation (2.79 gy/min) at room temperature (24 degrees c). t4+ was more sensitive to inactivation than was p22, and the t4os mutant was even more sensitive than t4+. catalase exhibited a strong protective effect and superoxide dismutase a weaker protection, indicating that h2o2 or some product derived therefrom was predominant in causing inactivation of plaqu ...	1988	3287426
[current status of radiotherapy following ablative and breast-sparing treatment of breast cancer].	after a total mastectomy in cases of a high risk of loco-regional recurrence the postoperative irradiation of the chest wall is indicated in the following situations: inflammatory type of carcinoma, tumor stage t3-t4, extended multifocal and multicentric primary tumor. after radical axillary surgery even in patients with positive nodes irradiation is not necessary except in cases where all axillary nodes are involved or with invasion of the axillary tissue. the supra- and infraclavicular lymph d ...	1988	3287443
[radiotherapy in the treatment of carcinomas of the base of the tongue. analysis of the literature and personal experience].	carcinoma of the base of the tongue is usually treated with radiation therapy. after a review of the literature, the findings are reported of 129 patients with squamous cell carcinoma of the base of the tongue treated in 1979-1983, with a minimum of a 3-year follow-up: 8 t1, 40 t2, 60 t3 and 21 t4 were found; only 48 cases were n0. advanced stages (stage iii and stage iv) were 83% of the total. external photon irradiation (cobalt-60) was used in all cases; 33 patients underwent chemotherapy or s ...	1988	3287497
exercise and the immune response.	a growing number of reports have become available which implicate infectious disease with reduced performance in athletes. the immune system consists of both nonspecific and specific components geared to control infections. adaptive immunity functions through both antibody-mediated and cell-mediated compartments to establish and maintain long term immunity to infectious agents. evidence is accumulating to support the view that physical exercise can lead to modification of the cells of the immune ...	1988	3287548
ph-adjusted 2-chloroprocaine for epidural anesthesia in patients undergoing postpartum tubal ligation.	the effect of added sodium bicarbonate on 2-chloroprocaine epidural anesthesia in women having postpartum tubal ligation was compared to the commercial product in a double-blind, randomized study. the rationale for the comparison was the reported hastened onset of anesthesia when the ph of bupivacaine and lidocaine are raised. anesthetic solutions were prepared no more than 20 minutes before use by adding 1 ml sterile 8.4% sodium bicarbonate or 1 ml sterile saline to 27 ml commercial product ( ...	1988	3288009
site-directed mutagenesis of the t4 endonuclease v gene: role of tyrosine-129 and -131 in pyrimidine dimer-specific binding.	t4 endonuclease v incises dna at the sites of pyrimidine dimers through a two-step mechanism. these breakage reactions are preceded by the scanning of nontarget dna and binding to pyrimidine dimers. in analogy to the synthetic tripeptides lys-trp-lys and lys-tyr-lys, which have been shown to be capable of producing single-strand scissions in dna containing apurinic sites, endonuclease v has the amino acid sequence trp-tyr-lys-tyr-tyr (128-132). site-directed mutagenesis of the endonuclease v gen ...	1988	3288278
the neuropathology of the acquired immune deficiency syndrome (aids). a review.	the nervous system has been involved in the majority (at least 75%), of cases of acquired immune deficiency syndrome (aids) examined postmortem, but the pathogenetic mechanisms involved are not well understood. the predominant pathological process is opportunistic infection secondary to the decrease of t-helper (t4) cells and includes toxoplasmosis, encephalitis due to cytomegalovirus and progressive multifocal leucoencephalopathy. on the other hand, mycoses (mainly cryptococcosis) are relativel ...	1988	3288294
evaluation of a new strategy for detection of thyroid dysfunction in the routine laboratory.	we assessed the use of a new strategy for detecting thyroid disorders, utilizing a sensitive assay for concentrations of thyrotropin (tsh) and free thyroid hormone in serum as follow-up tests. of 1279 patients who were not on thyroxin (t4) replacement treatment, 82% could be classified as euthyroid and would require no further tests. in patients who were on t4 replacement, 41% fell into the euthyroid category and would require no further tests. using this strategy to replace our existing strateg ...	1988	3288375
mutant 16s ribosomal rna: a codon-specific translational suppressor.	we have isolated an unusual codon-specific translational suppressor in escherichia coli. the suppressor resulted from a spontaneous mutation in a chromosomal gene during a selection for suppressors of the auxotrophic nonsense mutation trpa(uga211). the suppressor allows readthrough of uga mutations at two positions in trpa and at two sites in bacteriophage t4. it does not, however, suppress amber (uag) or ochre (uaa) mutations that were tested in both genomes, some of which were at the same posi ...	1988	3288986
promotive effect of 4,4'-methylenebis(n,n-dimethyl)benzenamine on n-bis(2-hydroxypropyl)nitrosamine-induced thyroid tumors in wistar rats.	the promotive effect of 4,4'-methylenebis(n,n-dimethyl)benzenamine (mdba) on 2-stage thyroid tumorigenesis induced by n-bis(2-hydroxypropyl)nitrosamine (dhpn) in wistar male rats was investigated. animals were given i.p. injections of dhpn, and then a basal diet containing 0.0375% mdba for 19 weeks. in addition, localization of the thyroid-stimulating hormone (tsh) in the pituitary gland was investigated. levels of tsh and t4 in serum were measured by radioimmunoassay (ria). the addition of mdba ...	1988	3289735
insulin effect on thyroid hormone uptake in rat skeletal muscle.	previous work has indicated that thyroid hormone entry into cells includes an energy-dependent, saturable process. in this study we investigated the effect of insulin on t3 uptake in rat skeletal muscle. intact soleus muscles were preincubated for 30 minuted at 37 degrees c, ph 7.4, in modified krebs-ringer bicarbonate buffer in the presence or absence of insulin and then for 60 minutes after adding 50 pmol/l [125i] t3. the results showed a stimulatory effect of insulin that was half maximal at ...	1988	3290621
the sonographical and functional sequelae of de quervain's subacute thyroiditis: long-term follow-up.	fifty-three patients with subacute thyroiditis (sat) were seen during the acute stage of the disease. hla-bw 35 was positive in 33 out of 39 tested patients. at first presentation, all examined patients (n = 23) had ultrasound abnormalities (generalized hypodensity, single or multiple hypodense areas). serum t4 and/or t3 were increased in 24/52, free t4 in 11/23, and the tsh response to trh was flat in 8/11 patients. six of 12 in whom volumetry was performed had goitres. thirty-seven patients we ...	1988	3291524
efficient synthesis of a supercoiled m13 dna molecule containing a site specifically placed psoralen adduct and its use as a substrate for dna replication.	we report a simple method for the in vitro synthesis of large quantities of site specifically modified dna. the protocol involves extension of an oligonucleotide primer annealed to m13 single-stranded dna using part of the t4 dna polymerase holoenzyme. the resulting nicked double-stranded circles are ligated and supercoiled in the same tube, producing good yields of form i dna. when the oligonucleotide primer is chemically modified, the resultant product contains a site-specific lesion. in this ...	1988	3291944
thyroxine and testosterone transcriptionally regulate renin gene expression in the submaxillary gland of normal and transgenic mice carrying extra copies of the ren2 gene.	expression of the mouse renin genes (ren1 and ren2) in the submaxillary gland of female mice has been analyzed following administration of thyroxine (t4) or dihydrotestosterone (dht). both hormones appear to act independently on mrna accumulation which increases about 5 fold over basal level. in vitro transcription assays in isolated nuclei demonstrate that both hormones act at the transcriptional level. the effects of dht and t4 were also analyzed in transgenic mice obtained by microinjection o ...	1988	3292291
an intensive, five course, alternating combination chemotherapy induction regimen used in patients with advanced, unresectable head and neck cancer.	progress in the treatment of advanced squamous cell cancer (scc) of the head and neck (h & n) has included the development of combination chemotherapeutic regimens that achieve impressive complete response (cr) rates using two to three courses therapy with minimal toxicity and good patient tolerance. further improvements in these results will require intensification of induction regimens. therefore, a five course, alternating combination chemotherapy induction trial was initiated in an attempt t ...	1988	3292714
length and shape variants of the bacteriophage t4 head: mutations in the scaffolding core genes 68 and 22.	the shape and size of the bacteriophage t4 head are dependent on genes that determine the scaffolding core and the shell of the prohead. mutants of the shell proteins affect mainly the head length. two recently identified genes (genes 67 and 68) and one already known gene (gene 22), whose products are scaffold constituents, have been investigated. different types of mutants were shown to strongly influence the proportion of aberrantly shaped particles. by model building, these shape variants cou ...	1988	3292792
[fluorescent immunoassay of thyroxine in blood serum].	a method of solid-phase fluoroimmunoassay of thyroxine (t4) on polystyrene 96-microtiter plates using coproporphyrin iii-labeled antibodies was developed; 0.02 ml of blood serum without preliminary treatment was required. thimerosal was added to lessen the t4-binding effect of the serum on the results of t4-binding effect of the serum on the results of the immunoassay. a calibration curve was obtained to determine t4 covering the entire physiological range of concentrations (from 3 to 21 microgr ...	1988	3293030
[immunological studies in hypersensitivity pneumopathies].	in immunological investigations in hypersensitivity pneumonia some have a diagnostic value for the clinician but many others are essentially of interest in research and in analysing the pathophysiology. unfortunately, skin tests with the responsible antigens are often positive in patients but also in exposed subjects without the lung disease. studies on peripheral blood require a search for ige immediate hypersensitivity which is rarely positive and above all a search for precipitating antibodie ...	1988	3293129
thyroid hormone effects on neonatal thermogenesis.	it is clear that thyroid hormones modulate non-shivering heat production of the newborn mammal. while some obvious thyroid hormone effects on thermogenesis can be demonstrated in adult, cold-acclimated or hibernating animals, these findings cannot be directly applied to the newborn, in which changes in amount and composition of brown adipose tissue, as well as sympathetic and thyroid system maturational events, are occurring. thyroid hormones do influence the prenatal development and subsequent ...	1988	3293226
fine-needle aspiration biopsy in the monitoring of liver allografts. i. correlation between aspiration biopsy and core biopsy in experimental pig liver allografts.	we have used allogeneic pig liver transplants to investigate the structure of inflammation in acute liver allograft rejection. an inflammatory episode of acute cellular rejection was observed in 9/10 allografts in nonimmunosuppressed recipients, when monitored with simultaneous fine-needle aspiration biopsies (fnab) and core needle biopsies (nb). the intensity of inflammation in fnab was quantitated using the corrected increment method and correlated with nb findings. in fnab, all inflammatory e ...	1988	3293284
fine-needle aspiration biopsy in the monitoring of liver allografts. ii. applications to human liver allografts.	serial fine-needle aspiration biopsies (fnab) were used for clinical monitoring of human liver allografts. nine liver allograft recipients were monitored with fnab at 1-3 day intervals. no complications were recorded. all patients underwent at least 1 inflammatory episode of acute rejection; altogether 11 episodes, all reversible, were recorded. the inflammatory infiltrate consisted mainly of lymphoid cells, including lymphoid blasts, with minor involvement of monocytes, monoblasts, and macropha ...	1988	3293285
[endocrine reaction pattern: midazolam-fentanyl anesthesia versus inhalation anesthesia].	in a randomised controlled study in 16 orthopaedic patients, the influence of midazolam-fentanyl-n2o/o2 anesthesia (group a) resp. halothane-n2o/o2 anesthesia (group b) on the plasma concentrations of the endocrine parameters acth, aldosterone, cortisol, 17-dhea, insulin, prolactin, t3, t4, tbg (thyroxine bounded globuline) as well as adrenaline, noradrenaline, and dopamine was investigated. additionally the metabolites glucose, lactate, free glycerin, and acetacetate were measured. beside prola ...	1988	3293479
dna strand exchanges.	biochemical and electron microscopic studies of the strand exchange reactions catalyzed by the reca protein of escherichia coli and the uvsx protein of t4 phage reveal that these reactions proceed in three distinct steps. the first step, termed joining, involves the assembly of reca (or uvsx) protein onto a single-stranded dna (ssdna) molecule and the subsequent search for homology with a double-stranded dna (dsdna) partner and formation of a stable synapsis. in the second step (envelopment/exch ...	1988	3293912
cloning, overexpression and purification of the terminase proteins gp16 and gp17 of bacteriophage t4. construction of a defined in-vitro dna packaging system using purified terminase proteins.	terminases of double-stranded dna bacteriophages are required for packaging and generation of terminii in replicated concatemeric dna molecules. genetic evidence suggests that these functions in phage t4 are carried out by the products of genes 16 and 17. we cloned these t4 genes into a heat-inducible ci repressor-lambda pl promoter vector system, and overexpressed them in escherichia coli. we developed an in-vitro dna packaging system, which, consistent with the genetic data, shows an absolute ...	1988	3294420
effect of different total parenteral nutrition fuel mixes on small intestinal growth and differentiation in the infant miniature pig.	insulin has been proposed as an important factor in the regulation of growth and differentiation of the small intestine. in the newborn miniature pig, we induced significant physiologic increases in serum insulin and the insulin/glucagon ratio without altering serum glucose, beta-hydroxybutyrate, glucagon, cortisol, t3, and t4 using glucose-based total parenteral nutrition (tpn) in one group (group g) compared with a combination of glucose and fat in another group (group g/f). control animals we ...	1988	3131182
thyroxine and triiodothyronine in plasma and thyroids of the neotenic and metamorphosed axolotl ambystoma mexicanum: influence of trh injections.	circulating levels of t3 and t4, as well as t3 and t4 content of the thyroid glands were measured by radioimmunoassay in the neotenic and metamorphosed axolotl ambystoma mexicanum. in the two experiments which were performed plasma t4 concentrations were more elevated in metamorphosed axolotls, especially in the first experiment (2.12 +/- 0.40 ng/ml vs. 369 +/- 30 pg/ml). t3 plasma values which were only estimated in the second experiment were about five times higher in metamorphosed animals (63 ...	1988	3131185
thyroxine surge in metamorphosing flounder larvae.	the tissue concentration of thyroxine (t4) in larval flounder (paralichthys olivaceus) was studied at various stages of their metamorphosis using a specific radioimmunoassay developed for samples obtained by quantitative extraction of the hormone from fish eggs and larvae. t4 concentrations were below the limit of detection in extracts of fertilized eggs and larvae until the end of prometamorphosis. several dorsal fin rays continued growing from premetamorphosis until the end of prometamorphosis ...	1988	3131186
the effects of the aids epidemic on the safety of the nation's blood supply.	present safeguards for the blood supply consist of three tiers of protection: donor deferral based on a donor's history of risk factors, confidential exclusion of blood units from donors with self-admitted risk factors, and testing of the blood itself. before the discovery of the aids virus in 1983 and 1984, there was no specific test relevant to aids that could be used to help improve the safety of the blood supply. the first step was intensified efforts, based on what was then known of the epi ...	1988	3131813
results of irradiation in squamous cell carcinoma of the soft palate and uvula.	out of a series of 235 patients presenting with tumours of the soft palate at the institut curie, between 1958 and 1980, 146 cases were analysed to evaluate the results of radical radiation therapy. seventy patients (48%) had advanced t3-t4 disease and 40 patients (27%) had clinically involved metastatic nodes. all patients had a minimum follow-up of 5 years. in 103 cases, megavoltage x-ray therapy was employed. for 43 patients, presenting with small or moderately advanced tumours, a combination ...	1988	3131842
requirements for phytohaemagglutinin activation of resting pure cd4+ and cd8+ t cells.	we have utilized a new method for obtaining highly purified cells using positive selection by immunomagnetic separation to study the conditions required for phytohaemagglutinin (pha) activation of pure t4 and t8 cells. in the presence of accessory cells (ac), a comparable proliferative response was obtained in the two subsets. in the absence of ac, pha induced low levels of interleukin 2 (il-2) receptor expression as well as responsiveness to il-2 in both t4 and t8 cells. if ac or 12-o-tetradeca ...	1988	3131868
[method for the determination of protein binding of thyroid gland hormones and the detection of antibodies to t4 and t3].	more than 99% of circulating thyroid hormones are bound with defined capacity and affinity to transport proteins such as thyroxine binding globulin, albumin and prealbumin. variants of albumin and/or prealbumin, often familial, will bind t4 and sometimes t3 with increased affinity causing higher tt4 and tt3 values respectively. in determining ft4 and ft3 by analogue tracer its binding to protein variants will also lead to falsely raised free parameters. thus the possibility of a false diagnosis ...	1988	3132024
pituitary and peripheral hormone responses to t3 administration during antarctic residence.	very little is known regarding hormonal adaptation in human subjects who are exposed to the extremes of temperature and light that are found in polar latitudes. we have previously reported a 50% elevation in the serum thyrotropin (tsh) response to thyrotropin-releasing hormone (trh), a fall in serum total triiodothyronine (t3) and free t3 (ft3), and no change in serum total thyroxine (t4) or free t4 (ft4) after 42 wk of antarctic cold exposure. to differentiate between central and peripheral mec ...	1988	3132047
site-directed mutagenesis of the t4 endonuclease v gene: role of lysine-130.	the dna sequence of the bacteriophage t4 denv gene which encodes the dna repair enzyme endonuclease v was previously constructed behind the hybrid lambda promoter olpr in a plasmid vector. the olpr-denv sequence was subcloned in m13mp18 and used as template to construct site-specific mutations in the denv structural gene in order to investigate structure/function relationships between the primary structure of the protein and its various dna binding and catalytic activities. the lys-130 residue o ...	1988	3132202
[serum thyrotropin concentrations in patients with sheehan's syndrome: reevaluation of the usefulness in the diagnosis].	serum concentrations of thyroid-stimulating hormone (tsh) were determined, using a highly sensitive immunoradiometric assay (irma), in 10 patients with sheehan's syndrome. serum tsh levels in these patients were from 2.3 to 9.0 microu/ml, with the mean of 6.4 +/- 2.3(sd) microu/ml, and the data were similar to those measured by a conventional ria method. the levels of serum tsh in these patients were normal or even higher than those of healthy women (1.8 +/- 1.3 microu/ml). after supplement ther ...	1988	3132405
thyroid function in severely traumatized patients with or without head injury.	the pattern of thyroid function changes following severe trauma was assessed prospectively in 35 patients during the first 5 days after injury. patients were divided into 2 groups to evaluate the effect of head injury: group i, patients with severe head injury; group ii, patients with multiple injuries without head injury. the results demonstrate a low t3 and low t4 syndrome throughout the study, with decreases in both total and free levels of t3 and t4, normal or increased rt3 levels, and norma ...	1988	3132788
suppressed plasma prolactin response to thyrotropin-releasing hormone in hyperthyroidism reproduced by thyroxine but not by triiodothyronine administration to normal subjects.	in 10 hyperthyroid women studied in the follicular phase of the menstrual cycle, basal plasma prl was normal, but prl release after trh was significantly suppressed compared with that in 11 control women. the suppressed prl response to trh was not explained by changes in serum estradiol or sex hormone-binding globulin. it recovered after treatment of hyperthyroidism. when normal women were treated with t4 (0.5 mg daily for 6 to 10 days), their mean serum free t4 level increased to about 70% of t ...	1988	3132791
t4+ cell production of interferon gamma and the clinical spectrum of patients at risk for and with acquired immunodeficiency syndrome.	to fully characterize the relationship between the clinical manifestations of human immunodeficiency virus infection and t4+ cell defects, we determined t4+ cell number and interferon gamma (ifn-gamma) production in 238 patients. for asymptomatic homosexuals, patients with acquired immunodeficiency syndrome (aids)-related complex (arc), and patients with fully established aids, clinical status correlated linearly with both t4+ cell number and t4+ cell-derived (antigen-stimulated) ifn-gamma secre ...	1988	3132901
effect of thyroid status and paraventricular area lesions on the release of thyrotropin-releasing hormone and catecholamines into hypophysial portal blood.	trh is a potent stimulator of pituitary tsh release, but its function in the physiological regulation of thyroid activity is still controversial. the purpose of the present study was to investigate trh and catecholamine secretion into hypophysial portal blood of hypothyroid and hyperthyroid rats, and in rats bearing paraventricular area lesions. male rats were made hypothyroid with methimazole (0.05% in drinking water) or hyperthyroid by daily injections with t4 (10 micrograms/100 g bw). untreat ...	1988	3133200
changes in the profiles of circulating white blood cells, corticosterone, t3 and t4 during the initiation of humoral immunity in immature male chickens.	eight-week-old male white leghorn chickens were injected intravenously with either 0.1 ml of brucella abortus (ba) or saline. blood samples were collected before injection, 1 hr, 3 hr, 6 hr, 12 hr, 24 hr, and then daily for the following 6 days after the injection. total white blood cells (wbc), differential counts and corticosterone (cs), t3 and t4 levels were measured in all blood samples. these data represented the results obtained from the primary response. to obtain data for the secondary r ...	1988	3133254
luteinizing hormone-releasing hormone as a potent stimulator of the thyroidal axis in ranid frogs.	plasma concentrations of t4, measured by radioimmunoassay, were raised significantly 2 and 4 hr after intravenous injection of synthetic luteinizing hormone-releasing hormone (lhrh) in rana ridibunda (1 and 10 micrograms on 2 consecutive days) and in rana esculenta (10 micrograms). a dose of 1 microgram lhrh was not so effective as 50 micrograms synthetic thyrotropin-releasing hormone (trh) when injected in rana ridibunda in november. however 10 micrograms lhrh was equipotent to 50 micrograms tr ...	1988	3133281
thyrotropic and peripheral activities of thyrotrophin and thyrotrophin-releasing hormone in the chick embryo and adult chicken.	the influence of an intravenous injection of thyrotrophin-releasing hormone (trh) and bovine thyrotrophin (tsh) on circulating levels of thyroid hormones and the liver 5'-monodeiodination (5'-d) activity is studied in the chick embryo and the adult chicken. in the 18-day-old chick embryo, an injection of 1 microgram trh and 0.01 i.u. tsh increase plasma concentrations of triiodothyronine (t3) and of thyroxine (t4). trh, however, preferentially raises plasma levels of t3, resulting in an increase ...	1988	3133299
investigation of the performance of the es 600 enzymun-test system. a multicentre study.	the es 600 sample-selective multibatch analyser was subjected to a multicentre evaluation in six laboratories in accordance with eccls guide-lines. during the 3-month trial, five enzymun-test diagnostics (t4, tbg, digoxin, cea and tsh)1) were measured at 25 degrees c. the study yielded the following results: 1. the within-series and between-series precision were very good, with mean cv's of approx. 3% and 7% respectively. 2. recovery of the target values for three control sera was in the range + ...	1988	3133447
decreased thyroidal response to thyrotropin in type ii diabetes mellitus.	the responses to trh and bovine tsh (btsh) were compared in 19 men with uncontrolled type ii diabetes mellitus and eight healthy control subjects. baseline serum tsh, t3 and t4 were similar in both groups and the rise of serum tsh, t3 and t4 following the intravenous (iv) administration of trh (500 micrograms) was not significantly different. diabetic subjects showed a blunted response to the subcutaneous (sc) administration of btsh (5 u) when their maximal serum t3 and t4 values were compared w ...	1988	3133539
analysis of human peripheral blood t lymphocytes proliferating in response to sensitizing antigens: effect of interleukin-2 (il-2)-responsive bystander cells.	the contribution of interleukin-2 (il-2)-responsive bystander cells to the proliferative responses of human peripheral blood t lymphocytes to antigens used for sensitization such as purified protein derivative (ppd), tetanus toxoid (t t) and influenza virus was investigated. marked proliferation of the unfractionated peripheral blood mononuclear cells (pbmc) was observed following stimulation with these antigens to which the individuals were known to have been sensitized previously. depletion of ...	1988	3134300
serum thyroid hormones, thyrotropin and thyroxine binding globulin during prolonged strength training.	the effects of progressive strength training for 24 weeks on maximal strength and pituitary-thyroid function were studied in 21 males during the training and during the following detraining period of 12 weeks. maximal strength increased greatly (p less than 0.001) in the first 20 weeks, followed by a plateau phase in the last 4 weeks of training. maximal strength decreased greatly (p less than 0.001) during the detraining period. the concentrations of serum total (t4) and free thyroxine (ft4) de ...	1988	3135185
thyroid hormone regulation of messenger ribonucleic acid encoding thyrotropin (tsh)-releasing hormone is independent of the pituitary gland and tsh.	cellular levels of mrna encoding pro trh in the rostral paraventricular nucleus are reduced by thyroid hormones. to determine whether this regulatory effect of thyroid hormones requires a functional pituitary gland or, specifically, tsh, we examined the effect of t3 on protrh mrna in hypophysectomized, thyro-parathyroidectomized male rats with or without bovine tsh replacement. hypophysectomy plus thyro-parathyroidectomy reduced serum t4 and tsh to undetectable levels in all animals and elevated ...	1988	3135488
epithelial hla-dr expression and lymphocyte subsets in gastric mucosa in type b chronic gastritis.	lymphocyte subpopulations (b, t4, t8), monocytes/macrophages (leum5, kim5) and hla-dr antigen expression were studied immunohistochemically in frozen sections from 32 antral and 37 fundal biopsies of type-b chronic gastritis. aberrant hla-dr antigen expression in epithelial cells of gastric mucosa was found in all cases closely related to mononuclear infiltrates. epithelial hla-dr expression and mononuclear infiltrates were practically absent in foci of intestinal metaplasia. these findings sugg ...	1988	3135657
letterer-siwe disease: immunohistochemical evidence for a proliferative disorder involving immature cells of langerhans lineage.	the morphological, ultrastructural and immunophenotypic properties of histiocytosis-x (h-x) cells were investigated in a lymph node involved by letterer-siwe (l-s) disease. h-x cells were t6+ (cd1a), s-100+, t4+ (cd4) and hla-dr+; in addition they were consistently t11+ (cd2) and were stained by antibodies directed against receptors for transferrin (t9), c3bi (okm-1/cd11b), igg-fc (leu-11/cd16) and interleukin-2 (il-2r/cd25). on immunostained cytosmears, t6+ cells were highly polymorphic and a p ...	1988	3135661
prolactin in patients with major depressive disorder and in healthy subjects. i. cross-sectional study of basal and post-trh and postdexamethasone prolactin levels.	prolactin (prl) levels were investigated in patients with major depressive illness and in healthy subjects. basal and postdexamethasone levels were measured in 27 patients, and levels after thyrotropin-releasing hormone (trh) stimulation (delta prl) were measured in 22 patients. basal and delta prl were also determined in 64 age- and sex-matched healthy subjects. both basal and postdexamethasone prl levels were normal in depressed patients, with the postdexamethasone levels in particular showing ...	1988	3135847
anterior pituitary type ii thyroxine 5'-deiodinase activity is not affected by lesions of the hypothalamic paraventricular nucleus which profoundly depress pituitary thyrotropin secretion.	bilateral destruction of the hypothalamic paraventricular nuclei (pvn) produced a profound depression of plasma tsh and the median eminence trh concentration in hypothyroid rats. anterior pituitary type ii iodothyronine 5'-deiodinase (5'-d) activity was consistently lower but not significantly different in sham- and pvn-lesioned rats. treatment with suboptimal replacement doses of 0.15 and 0.75 micrograms t4/100 g bw.day produced a graded depression of plasma tsh in the pvn (p less than 0.02), b ...	1988	3136010
[thyroid hormone metabolism in nonthyroidal illnesses. ii. a new method for measuring serum thyroid hormone binding inhibitor and its clinical application].	it is well known that serum t3 and t4 concentrations are often decreased in patients with nonthyroidal illnesses (nti). the purpose of this study was to elucidate the possible role of serum thyroid hormone binding inhibitor (thbi) in lowering serum t4 in patients with nti using the originally developed method for measuring thbi. 125i.t4 and 0.05 micromilligram of pooled normal serum was applied to the sephadex g.25 column equilibrated in advance by 0.1n naoh, and 4 micromilligram of 0.075m veron ...	1988	3136041
platelet mao activity, trh test, and dexamethasone suppression test in depressed patients.	platelet mao activity, trh test and dst results were compared in 23 normal subjects and 13 depressed patients. patients as a group were older in age, had higher levels of ft4-index and t3-uptake, and lower levels of mao activity. there was no association between thyroid hormones, including tsh response, and mao activity. five patients were dst positive. they showed a higher average age and a higher t3-uptake value than dst negative patients. serum t4 and mao activity levels were normal in both g ...	1988	3136464
studies of transformer repair workers exposed to pcbs: ii. results of clinical laboratory investigations.	thirty-eight transformer repairmen currently exposed to polychlorinated biphenyls (pcbs), 17 former transformer repairmen, and 56 comparison workers not known to be exposed to pcbs were studied. measurements were made of serum liver function tests, gamma-glutamyl transpeptidase (ggt), lipid profile, thyroid function tests, and other serum biochemistry; hemoglobin; white cell count; 24-hour excretion of delta-aminolevulinic acid, porphyrins, 17-hydroxycorticosteriods and 17-ketosteroids; sperm co ...	1988	3136647
differential binding of thyroxine and triiodothyronine to acidic isoforms of thyroid hormone binding globulin in human serum.	the differential availability of thyroxine (t4) and 3,5,3'-triiodothyronine (t3) to liver from the circulating thyroid hormone binding globulin (tbg)-bound pool suggests that the two thyroid hormones may bind to different tbg isoforms in human serum. in the present study, the binding of [125i]t4 and [125i]t3 to human serum proteins was investigated by using slab gel isoelectric focusing and chromatofocusing. in normal human male serum, [125i]t4 was localized to four isoforms of tbg called tbg-i, ...	1988	3136801
tsh response to trh and haloperidol response latency in psychoses.	thyroid-stimulating hormone (tsh) response to thyrotropin-releasing hormone (trh) was measured in 19 acutely psychotic (dsm-iii schizophrenia, 7; schizophreniform, 2; schizoaffective, 3; affective, mood-incongruent psychosis, 5; manic, mood-congruent psychosis, 2) drug-free patients prior to systematic trials of lithium and/or haloperidol. tsh response was not associated with sex, age, baseline t4, or baseline tsh. a reduced tsh response was associated with affective diagnosis and was a signific ...	1988	3136807
the influence of diabetes mellitus on thyrotropin response to thyrotropin-releasing hormone in untreated acromegalic patients.	impairment of thyrotropin (tsh) response to thyrotropin-releasing hormone (trh) has been documented in patients with uncontrolled diabetes mellitus (dm). in acromegalic patients, however, there have been no data regarding tsh secretion studied taking the existence of dm into consideration. therefore, we investigated the tsh response to trh [expressed as tsh increment (delta tsh)] in 14 untreated acromegalic patients, who did not show the suprasellar extension of adenoma, divided into two groups ...	1988	3137252
[bacteriophages of pseudomonas putida containing single-stranded canonical dna breaks].	it was shown that bacteriophage tf as well as bacteriophages phi p4/40, phi p25/42, phi p23/40 and phi p6/40, which are specific to different p. putida strains, contain the single strand breaks in their dna. the breaks are localized in one strand of dna molecules and are repairable with t4 dna ligase. bacteriophage tf has no detectable dna homology with phi p4/40, phi p25/42, phi p23/40 and phi p6/40 bacteriophages. all the phages studied have no relation with other known pseudomonas phages. bac ...	1988	3137462
amp-dependent dna relaxation catalyzed by dna ligase occurs by a nicking-closing mechanism.	in the presence of amp and mg2+, a covalently closed duplex dna containing negative superhelical turns was treated with dna ligase isolated from bacteriophage t4-infected e. coli. this resulted in the gradual and not sudden loss of superhelical turns as for example in the case of type i dna topoisomerase. all dna products remain covalently closed. since t4 enzyme-mediated dna relaxation is inhibited by both pyrophosphate and by atp this suggests that dna relaxing and dna joining activities proba ...	1988	3137526
[basal and trh stimulated tsh secretion and determination of total thyroxine (t4). thyroxine-binding capacity and free thyroxine index (ft4-i) in patients with chronic uremia].	in 11 patients with chronic uremia both the basal and trh stimulated tsh levels and t4, tbc and ft4-i were determined. the investigations were repeated in 2 cases after renal transplantation. tsh and t4 in serum were determined by ria, tbc by radio reagent assay. ft4-i was calculated. in 8 patients the basal tsh levels were in the normo- and in 3 in the hypothyreotropic range. in 9 patients the response to trh was adequate. there were deviations from the physiological range in 7 patients for t4 ...	1988	3137736
immunohistochemical studies on intrahepatic lymphocyte infiltrates in chronic type b hepatitis, with special emphasis on the activation status of the lymphocytes.	to study the immunopathogenesis of chronic type b hepatitis, we used a panel of monoclonal antibodies to characterize the intrahepatic mononuclear cell infiltrates in 39 patients with various forms of chronic hepatitis induced by hepatitis b virus (hbv) and, for comparison, in 10 with non-a, non-b (nanb)-induced chronic hepatitis. in portal areas, the okt4:okt8 ratio reversed because of the relative increase of okt8+ cells. the mean intraportal t4:t8 ratio was significantly lower in hbv-associat ...	1988	3137808
direct determination of free thyroxin in undiluted serum by equilibrium dialysis/radioimmunoassay.	we have designed a re-usable dialysis cell and a complex dialysis buffer, with which undiluted serum samples can be dialyzed with minimal changes in their serum matrix. dialysate thyroxin (free t4) is then measured by a sensitive ria for t4. the range of reportability was 2-128 ng/l, the normal range was 8-27 ng/l, and the interassay cv was 7%. free t4 concentrations in various disorders were as follows: hyperthyroidism, 32-478 ng/l; in both excess thyroxin-binding globulin (tbg) and familial dy ...	1988	3138040
new one-step enzyme immunoassay for free thyroxin.	this enzyme immunoassay for free thyroxin (ft4) involves simultaneous incubation of sample and thyroxin conjugated to horseradish peroxidase (ec 1.11.1.7) in antibody-coated tubes at room temperature. the test can be performed in 90 min. it is not influenced by variations in concentrations of thyroxin-binding globulin (tbg), thyroxin-binding prealbumin, or albumin. with increased concentrations of non-esterified fatty acids, the measured ft4 equivalent concentrations increase immediately, indica ...	1988	3138045
effects of trh on hormone release from pituitaries of the lizard, anolis carolinensis.	the ability of thyrotropin-releasing hormone (trh) to stimulate thyrotropin (tsh) from pituitaries of adult male lizards, anolis carolinensis, was tested in vivo and in vitro. tsh output by pituitaries in vitro was determined by bioassay of the incubation medium using in vitro t4 output by thyroids from the same lizards. pituitaries incubated without trh had no detectable tsh secretion during two consecutive (2 or 3 hr) periods of incubation. incubation in 10 or 100 ng/ml trh for 2 or 3 hr signi ...	1988	3138157
regression after 50 gy as a selection for therapy in advanced laryngeal cancer.	radiation alone for advanced laryngeal cancer will result in an initial local control rate of 50%. when a local recurrence is diagnosed, only 50% will be successfully salvaged by surgery. to identify patients with a high chance of local control with radiation alone in advanced laryngeal cancer, the clinical response following radiotherapy was assessed a few days after 50 gy/5 weeks. in patients with t-stage reduction or greater than 50% tumor regression radiotherapy was continued, if not, laryng ...	1988	3138211
radiation therapy of the nasopharyngeal cancer and its prognostic factors.	39 patients with the regional nasopharyngeal cancer (npc) underwent radiation therapy from 1975 through 1984 in the department of radiology, chiba university hospital. a retrospective study was carried out to find out prognostic factors of these npc patients. radiation therapy was performed under megavoltage condition. 31 patients were given more than 50 gy. 41% of the whole had t4 extension and 69% had lymph node metastasis. the pathological slides were reviewed and classified by the proposal o ...	1988	3138767
effects of low dose oral iodide supplementation on thyroid function in normal men.	previous studies have demonstrated that short-term oral iodide administration, in doses ranging from 1500 micrograms to 250 mg/day, has an inhibitory effect on thyroid hormone secretion in normal men. as iodide intake in the usa may be as high as 800 micrograms/d, we investigated the effects of very low dose iodide supplementation on thyroid function. thirty normal men aged 22-40 years were randomly assigned to receive 500, 1500, and 4500 micrograms iodide/day for 2 weeks. blood was obtained on ...	1988	3139337
decreased growth hormone (gh) response to oral clonidine in endemic cretinism: effect of l-t3 therapy.	as gh secretion is dependent upon thyroid hormone availability, the gh responses to clonidine (150 micrograms/m2) and the tsh and prl response to trh were studied in eight endemic (ec) cretins (3 hypothyroid, 5 with a low thyroid reserve) before and after 4 days of 100 micrograms of l-t3. five normal controls (n) were also treated in similar conditions. both groups presented a marked increase in serum t3 after therapy (n = 515 +/- 89 ng/dl; ec = 647 +/- 149 ng/dl) followed by a decrease in basal ...	1988	3139739
free thyroxine values in dried blood spots on filter paper in newborns are related to both gestational age and birth body weight.	the results of free thyroxine (ft4) measurements in dried blood spots on filter paper in 744 euthyroid newborns (616 at term, 128 preterm), 10 newborns with congenital hypothyroidism and 4 euthyroid newborns with congenital tbg deficiency are reported. ft4 was measured by column adsorption chromatography of free hormone followed by radioimmunoassay in the eluate. ft4 values averaged 24 +/- 0.2 pmol/l (mean +/- se) in euthyroid newborns, 23.0 +/- 0.9 pmol/l in euthyroid newborns with tbg deficien ...	1988	3139742
initial characterization of a lymphokine pathway for the immunologic induction of tumor necrosis factor-alpha release from human peripheral blood mononuclear cells.	under endotoxin-free conditions, unstimulated human pbmc do not release tnf-alpha, as measured in a sensitive assay with 51cr release in 6 h from actinomycin d-treated wehi 164 cells. ifn-gamma alone at less than or equal to 10,000 u/ml is insufficient to elicit tnf-alpha release. similarly, the lymphokine-rich supernatant of pbmc stimulated by allogeneic cells is also insufficient to induce tnf-alpha release in a short term assay. however, when pbmc are first primed with ifn-gamma for 48 h and ...	1988	3139748
hypothalamic hormones that release growth hormone stimulate hepatic 5'-monodeiodination activity in the chick embryo.	plasma gh, tri-iodothyronine (t3), thyroxine (t4) and liver 5'-monodeiodination (5'-d) activity were measured in 18-day-old chick embryos injected with thyrotrophin-releasing hormone (trh) and human pancreatic growth hormone releasing factor (hpgrf). injections of 0.1 and 1 microgram trh and 1.5 micrograms hpgrf increased the concentration of plasma gh while injection of 15 micrograms hpgrf had no effect. concentrations of plasma t3 were raised after injection of trh or hpgrf. injections of trh ...	1988	3139815
[anorexia nervosa in male adolescents. ii. psychoneuroendocrinologic findings].	female patients with anorexia nervosa (a.n.) are characterized by distinct endocrine features probably due to hypothalamic pituitary dysfunctions. there is only a limited number of case reports available on patients with a.n.; mostly with few data on hormones. in six male patients with a.n. we examined basal and stimulation values of several hormones performing three pituitary function tests. basal and stimulated values of luteinizing hormone (lh) and of follicle stimulating hormone (fsh) after ...	1988	3139910
paracoccidioidomycosis in the region of botucatu (state of são paulo, brazil). evaluation of serum thyroxine (t4) and triiodothyronine (t3) levels and of the response to thyrotropin releasing hormone (trh).	t4, t3 and tsh serum levels were measured in 25 patients with paracoccidioidomycosis. thyroid t3 reserves were measured on the basis of the increase in t3 (delta t3) 2 h after intravenous injection of 200 micrograms trh, and pituitary tsh reserves were measured on the basis of tsh increase (delta tsh) 20 min after the same injection. twenty healthy volunteers with no history of thyroid disease were used as controls. when the two groups were compared, the following results were obtained: (a) ther ...	1988	3140015
flavonoid effects on transport, metabolism and action of thyroid hormones.	flavonoids are potent non-toxic ith deiodinase inhibitors in microsomal membranes, and intact rat hepatocytes. flavonoids are specific high affinity competitors for l-t4-binding to human tbpa, weaker antagonists in the rt3-5'deiodinase reaction, and very poor inhibitors of t3 binding to the nuclear t3 receptor. optimized flavonoid antagonists exhibit an ith bio-isosteric structure and conformation. the t4-binding site of htbpa is a useful "receptor model" for type i deiodinase in computer aided ...	1988	3140249
pituitary-thyroid interaction: effects of thyroid hormone, non thyroidal illness and various agents on tsh secretion.	recent developments in thyroid hormone metabolism have helped to understand the complex events which characterize the regulation of tsh secretion. plasma t3 concentration as well as intrapituitary t3 generation from t4, exert a profound effect on tsh synthesis and release. pituitary type ii deiodinase differs from type i deiodinase found in other tissue such as liver and kidney, and in fact different conditions such as hypothyroidism and hyperthyroidism affect these enzymes in opposite direction ...	1988	3140559
3,3',5-l-triiodothyronine but not l-thyroxine can block the induction of tyrosinase by imidazole in cultured b16 melanoma cells.	3,3',5-l-triiodothyronine (t3) can inhibit tyrosinase activity in b16/c3 melanoma cells in culture and block the induction of that enzyme by imidazole (endocrinol. 119, 2118, 1986). the current study examined the effect of thyroxine (t4) on tyrosinase activity. proliferating cultures were exposed to either t3 (0-500 nm) or t4 (0-50,000 nm) in addition to imidazole (10 mm). imidazole induced enzyme activity by approximately 3-fold and t3 could block that induction with a maximal inhibition occurr ...	1988	3140808
abnormalities in thyroid function associated with chronic therapy with methadone.	we studied 145 clinically euthyroid patients on maintenance methadone therapy for narcotic withdrawal, to characterize abnormalities in thyroid-function tests induced by methadone. about a third had increased total thyroxin (t4) and total triiodothyronine (t3) concentrations in serum. the mean concentrations of t3, t4, and thyroxin-binding globulin (tbg) in serum were significantly greater (p less than 0.001 each) in these patients than in a euthyroid control group. there was a corresponding dec ...	1988	3141082
age-related changes in the response of hepatic succinic dehydrogenase activity to thyroxine and thiourea in lizards.	intraperitoneal injection of thyroxine (t4; 1 microgram/g body weight on alternate days, seven doses) to male garden lizards of three different age groups caused a stimulation of hepatic succinic dehydrogenase activity, the degree of response being higher in the old (63%) than in middle-aged (39%) and young (31%). percent inhibition of enzyme activity following thiourea treatment (0.05 mg/g body weight on alternate days, three doses) was higher in middle-aged lizards (24%) than in young (10%) an ...	1988	3141248
thyroid hormone response to thyrotropin releasing hormone after cold treatment during pre- and postnatal development in the domestic fowl.	the purpose of the present study was to compare the effect of periodic cooling during the establishment of a functional pituitary-thyroid axis at days 11-14 of incubation and at other developmental stages, on the subsequent thyroid hormone response to thyrotropin releasing hormone (trh). in the first and second experiment chick embryos were cooled for 6 hr/day to 30 degrees c from day 11 till 14 and from day 15 till 18 respectively, whereas control groups were incubated throughout at 37.8 degree ...	1988	3141256
prolactin and thyroid status in prepubertal children with mild to moderate obesity.	prolactin (prl) response to thyrotropin releasing hormone (trh) in 21 prepubertal children with mild to moderate obesity was compared with that in 21 normal prepubertal children (controls). basal prl levels were normal but the mean peak prl response and mean increment in prl levels following trh administration were significantly lower in prepubertal obese children (p less than 0.001). the mean prl responses to trh were significantly impaired at all time intervals in prepubertal obese boys and gi ...	1988	3141493
lack of acute d-amphetamine effects on thyrotropin release.	case reports have suggested that amphetamine abuse causes excessive secretion of thyrotropin (tsh) and thyroxine (t4). such an amphetamine-induced effect might be noradrenergic-mediated in the hypothalamus. the current controlled study examined oral d-amphetamine effects on the hypothalamic-pituitary-thyroid axis in normal humans. no acute effects were seen on tsh, t3 or t4 levels. d-amphetamine elevated cortisol levels at 180 min, as previously reported.	1988	3141826
thyroid and liver trophic changes in rats secondary to liver microsomal enzyme induction caused by an experimental leukotriene antagonist (l-649,923).	thyroid hyperplasia and/or hepatomegaly were observed in a 14-week oral toxicity study with l-649,923, a leukotriene antagonist, at doses of 50 and 150 mg/kg/day. in a 16-day study, l-649,923 caused an increase in plasma tsh and hepatic enzyme induction, but did not affect plasma t3 and t4 levels. light microscopy and ultrastructural examination of the liver and thyroid showed changes indicative of hepatic enzyme induction and increased stimulation of the thyroid by tsh. because other hepatic en ...	1988	3142097
thyroid hormones and thyroxine-binding globulin in relation to liver function and serum testosterone in men with alcoholic cirrhosis.	in 73 euthyroid male patients with histologically verified alcoholic cirrhosis, thyroid hormones, thyroxine-binding globulin (tbg) and testosterone concentrations (total, non-protein- and non-shbg-bound) were studied in relation to each other and to the degree of liver dysfunction. serum concentrations of triiodothyronine (t3) decreased significantly (p less than 0.05) and thyroid-stimulating hormone (tsh) increased with progressing liver dysfunction. serum concentrations of tetraiodothyronine ( ...	1988	3142221
serum concentrations of thyroxine and 3,5,3'-triiodothyronine in dogs before and after administration of freshly reconstituted or previously frozen thyrotropin-releasing hormone.	concentrations of serum thyroxine (t4) and 3,5,3'-triiodothyronine (t3) were determined after the administration of freshly reconstituted thyrotropin-releasing hormone (trh), reconstituted trh that had been previously frozen, or thyrotropin (tsh) to 10 mature dogs (6 greyhounds and 4 mixed-breed dogs). thyrotropin-releasing hormone (0.1 mg/kg) or tsh (5 u/dog) was administered iv; venous blood samples were collected before and 6 hours after administration of trh or tsh. concentrations of the t4 ...	1988	3142321
influence of stationary and moving textured backgrounds on the response of visual neurons in toads (bufo bufo l.).	previous studies have shown that configurational prey recognition in common toads is performed by feature-analyzing functional units consisting of assemblies of connected neurons such as retinal (classes r2, r3), tectal [classes t5(1), t5(2), t5(3)], and pretectal (class th3) cells. in the present paper, effects of textured backgrounds on the response of these neurons to a configurational moving stimulus have been tested quantitatively. (1) in all investigated neurons, neither the overall activa ...	1988	3142636