Publications

[influence of microflora on the metabolism of l-threonine at the level of the rat digestive tract].	intestinal tissues are found to incorporate l threonine 14cu at faster rate in the absence of microflora. most of the radioactivity of the digestive contents occurs in the tca soluble compounds, except in the coecum and large intestine of the conventional rats where the microflora synthetized its own proteins from the labelled digestive material. free threonine is the main labelled soluble compound in the conventional rats digestive contents. in the other hand, in the germfree rats digestive con ...	1976	134804
[blood on filtration paper for determination of rubella haemagglutination-inhibiting antibodies (author's transl)].		1976	134837
glucocorticoid and starvation effect on glycosaminoglycans in vascular connective tissue. biochemical studies on repair processes in rabbit aorta.	male rabbits were injured by a single mechanical dilatation injury of aorta and then injected with prednisone 2 mg/kg or saline for 14 days or subjected to starvation. the biosynthesis of the sulfated glycosaminoglycans as evaluated by the uptake of 35s-sulfate and the content of the glycosaminoglycans were measured on the intima-media layer of the descending thoracic aorta. the results indicate that prednisone may inhibit the biosynthesis of heparan and/or dermatan sulfate while starvation incr ...	1976	134864
a stabilised tris(hydroxymethyl)aminomethane adduct in reduced collagen.	the reduction of collagen with sodium [3h] borohydride in the presence of tris buffer results in the stabilization of a schiff base adduct which is formed between allysine residues and tris(hydroxymethyl)aminomethane. the reduced, radioactive derivative of this adduct has been identified in hydrolyzates or reduced collagen. it elutes before hydroxylysine on an amino acid analyzer column close to the position of dihydroxylysinonorleucine. similar artifacts may occur when aldehydes present in or a ...	1976	134871
the subunits of rabbit-muscle phosphofructokinase. a search for sequence repetition.	rabbit muscle phosphofructokinase uniformly carboxymethylated with iodo[2-14c]acetate consists of subunits with a molecular weight of 80 000 +/- 5000. the subunit polypeptide chain contains 16 and 52 residues respectively of cysteine and arginine and, contrary to previous results, peptide mapping experiments gave no indication that phosphofructokinase chains yield fewer than the expected numbers of cysteine and arginine containing peptides. to test further for the possible occurrence of repeat s ...	1976	134894
secretory iga against enterotoxins in breast-milk.	a pool of colostrum from guatemalan mothers (guatemalan colostrum)) obtained 2-4 days post partum inhibited the induced fluid accumulation in rabbit ileal loops when incubated with vibrio cholerae or escherichia coli enterotoxin. there was a linear relationship between the quantity of colostrum used and the protection achieved. pools of guatemalan breast-milk obtained 15-30 days post partum and north american breast-milk had the same effect when tested with e. coli and v. cholerae enterotoxins, ...	1976	73692
experimental cancer immunotherapy: modification of tumor cells to increase immunogenicity.	firmly established transplantable c3h/hej mammary carcinomas can be inhibited by host challenge with vibrio cholerae neuraminidase (vcn)-treated tumor cells. the effect is totally immunospecific, even vcn-treated tumors bearing shared mammary tumor virus (mtv) antigen cannot induce the regression. thus, vcn is capable of increasing the immunogenicity of the private, unique-unshared tumor antigens on mammary carcinomas; vcn is incapable of increasing the immunogenicity of the shared mtv-associate ...	1976	68699
[methodical recommmendations for conduction of the hemagglutination test with erythrocytic cholera enterotoxic diagnostic agent (eceda)].		1976	66381
a serial study of pregnancy proteins in primigravidae.	the plasma concentrations from four 'pregnancy proteins' and three steroid hormones have been measured throughout pregnancy in 15 primigravidae. two of the proteins, human placental lactogen (hpl) and pregnancy-specific beta1-glycoprotein (psbetag), are specific for pregnancy and correlate well with the stage of gestation. it is suggested that measurement of psbetag may be useful in assessing feto-placental wellbeing. neither of the pregnancy-associated proteins, steriod-binding beta-globulin (s ...	1976	57799
[ultrastructural analysis of preparations impregnated with silver salts].	the work was devoted to the ultrastructural analysis of the neurohistological preparations. sections of the tissue from the precardial parts of the pulmonary and caval dog veins were impregnated with silver salts after campos and embedded in the araldite by a special method. electronmicroscopi studies showed reduced silver adsorbed by the tissue of the impregnated preparations to exhibit a granular structure (the granules were 30-400 a in size). the largest of them were revealed in the axoplasm ...	1976	55281
fetal syphilis in the first trimester.	evidenc of first-trimester fetal syphilis was sought in the products of conception in a therapeutic abortion clinic. during two collection periods of one week, five patients with serologic and clinical data consistent with recent syphilitic infection were identified. their conceptuses were carefully examined by silver and immunofluorescent stains for the presence of treponema pallidum. two of these five conceptuses (9 and 10 weeks' gestation) were found to contain t. pallidum by these methods. t ...	1976	56895
differential mechanisms of increased alpha 1-fetoprotein production in rats following carbon tetrachloride injury and partial hepatectomy.	possible differences in the mechanisms of increased alpha1-fetoprotein (afp) production following carbon tetrachloride (ccl4) intoxication and partial hepatectomy were studied with 5-week-old rats at the time of sacrifice. the maximum level of serum afp reached in 4 days after a single dose of ccl4 was much higher than that after partial hepatectomy, although the incorporation of [3h]thymidine into liver dna increased nearly to the same extent by either of these treatments. in the remnant after ...	1976	58715
induction of tryptophanase in nitrofurantoin resistant vibrio el tor.		1976	59523
amino acid metabolism and protein synthesis in streptomycin resistant vibrio el tor.	metabolic activities in relation to protein synthesis and amino acid utilization are altered in vibrio el tor after development of resistance towards streptomycin. efficiency of in vivo and in vitro protein synthesis is markedly reduced in streptomycin resistant vibrio el tor. the rate of incorporation of 14c-amino acids into protein, uptake of 14c-valine and oxidation of certain amino acids are also altered.	1976	59524
small fragments from the a subunit of cholera toxin capable of activating adenylate cyclase.	exposure of cholera toxin to membrane particles prepared from sarcoma 180 cells gives rise to a variety of fragments which are capable of activating adenylate cyclase [atp:pyrophosphate-lyase (cyclizing), ec 4.6.1.1]. a major component of these fragments has an apparent molecular weight in the 8,000-10,000 range. the smallest stimulatory fragment has a molecular weight of approximately 1400. the small size of the fragments is confirmed by sephadex gel filtration, in the presence of either sodium ...	1976	60760
[production and use of a cholera antigenic erythrocyte diagnostic agent].		1976	60852
macrophage plasminogen activator: modulation of enzyme production by anti-inflammatory steroids, mitotic inhibitors, and cyclic nucleotides.	plasminogen activator production by cultured mouse peritoneal macrophages can be modulated in vitro by low concentrations of various pharmacologically active molecules. glucocorticoid hormones and their synthetic derivatives, as well as cholera toxin, colchicine, and vinblastine markedly inhibit production of this enzyme without affecting other important macrophage functions. the effect of glucocorticoids is of particular interest, both because their relative in vivo anti-inflammatory potencies ...	1976	61067
reverse plaque formation by hog cholera virus of the gpe-strain inducing heterologous interference.	a simple and rapid plaque procedure was developed for the assay of hog cholera virus (hcv) of a particular strain, gpe-, based on its intrinsic interference with vesicular stomatitis virus (vsv) on the primary swine testicle cells and on an established swine kidney cell line; the procedure is called the reverse plaque formation (rpf) method. the plaques were produced as colonies of hcv-infected cells which were vsv-sensitive, disintegrated cell sheet. these plaques became visible after 15 to 20 ...	1976	61176
[rapid method of determination of sensitivity of vibrio cholerae to antibiotics].		1976	61330
is cholera primarily water-borne.		1976	62178
cholera, copepods, and chitinase.		1976	62179
[antigenic and serologic properties of yersinia enterocolitica].		1976	62475
cholera is primarily waterborne in bangladesh.		1976	63781
[the effect of increased intracranial pressure and subarachnoid hemorrhage on the sympathetic vasoconstrictor nerve activity (author's transl)].		1976	64942
effect of cholera enterotoxin preparations on cutaneous response in rabbit under varied conditions.	enterotoxic activity of two preparations obtained from vibrio cholerae, b-53-6 inaba and b-53-10 ogawa was tested in ligated ileal loops of rabbit. the biologically active enterotoxic preparations were further used to study the permeability reaction in rabbit skin. cutaneous response tended to be linear only with higher concentrations of the toxin and showed maximum blueing intensity between 16 and 24 hrs of intracutaneous inoculation. exposure of enterotoxin preparations to elevated temperature ...	1976	13591
the arrangement of subunits in cholera toxin.	cholera toxin consists of five similar b subunits of apparent molecular weight about 10 600 and one a subunit (29 000) consisting of two peptides (a1 23 000-24 000 and a2 about 5500) linked by a single disulfide bond. each b subunit also contains one internal disulfide bond which is readily reduced but is protected from carboxymethylation unless the reduced subunits are heated in urea. tyrosine residues in a1 and in b subunits are readily iodinated, but the intact b assembly does not react with ...	1976	3214
properties of cholera toxin- and naf-stimulated adenylate cyclase from mouse thymocytes.	kinetic parameters of mouse thymocyte adenylate cyclase activity were determined. naf and cholera toxin stimulated adenylate cyclase. stimulation by either agent did not change the ph or mg2+ optima relative to control (unstimulated cyclase). the km value for atp of adenylate cyclase stimulated by naf was significantly reduced from control. by contrast, cholera toxin treatment did not change the km relative to control. adenylate cyclase, when stimulated by naf, had an optimum for mn2+ alone, or ...	1976	3217
interaction of cholera toxin and toxin derivatives with lymphocytes. ii. modulating effects of cholera toxin on in vivo humoral and cellular immune responses.	the in vivo effects of cholera toxin on lymphoid organ structure and function in mice were investigated. it was found that within a day following intravenous injection of 1 mug of toxin, thymus as well as spleen weight decreased but the animals remained healthy. histological studies suggested that the involution of lymphoid organs was due to cell death. injection of cholera toxin into adrenalectomized mice was lethal within 36 h. in these animals no decrease in lymphoid organ weight was noted. t ...	1976	5378
comparative study of the proteinograms of vibrios and of closely related microorganisms by means of polyacrylamide gel disc electrophoresis.	proteinograms of 112 strains of vibrios and closely affiliated microorganisms were studied by disc electrophoresis in polyacrylamide gel. up to 25 protein peaks with definite mobility coefficients were revealed. the influence of the culture medium on the protein spectrum of the microbes was found. the frequency of peak formation was of great significance for the differentiation of the microbes under study. the quantitative characteristics of the peak area could not be used for differentiation.	1976	7908
molecular weight analysis of the polypeptides of cholera phage pl 163/10 by polyacrylamide gel electrophoresis.	cholera phage pl 163/10, belonging to mukerjee's group i, was purified by alternate cycles of low and high speed centrifugation. gel electrophoresis revealed the presence of four polypeptide chains of respective molecular weights of 10,370+/-515 (a), 30,000+/-1,303 (b), 40,000+/-1,049(c) and 64,000+/-2,433 (d) daltons. electrophoresis of the sample alkylated with iodoacetic acid resolved the presence of only one polypeptide chain of an average molecular weight of 10,310+/-565 daltons. the polype ...	1976	9816
use of thyrotropin and cholera toxin to probe the mechanism by which interferon initiates its antiviral activity.	thyrotropin (10 mum) inhibited the antiviral activity of interferon. when added after interferon, thyrotropin (tsh) had no effect on antiviral activity. there was also no inhibition of interferon action in cells washed with medium between incubations with tsh and interferon. 125i-labeled tsh and 125i-labeled cholera toxin could bind to preparations of mouse l-cell plasma membranes. the binding was specific in that it was prevented by unlabeled thyrotropin or cholera toxin, but not by insulin, gl ...	1976	10573
antibacterial activity of ambodryl and benadryl.		1976	11202
effect of caffeine on the motility, viability, oxygen consumption and glycolytic rate of ejaculated human normokinetic and hypokinetic spermatozoa.	in this report the effect of caffeine, ibmx and cholera toxin on ejaculated human spermatozoa was tested. it was found that caffeine was the most effective drug, and that cholera toxin was ineffective. caffeine stimulated motility, preserved viability and increased the glucose, fructose utilization and lactate production of sperm in whole semen and washed sperm. oxygen consumption however was decreased. these results of the normokinetic ejaculates were defined as a basis for comparison with the ...	1976	12119
gas levels and biochemical changes in cerebrospinal fluid after experimental brain injury: its effect on cerebral circulation and cerebral metabolism as well as its management.	1. after brain damage experimentally induced by balloon compression, liquor of the cistern shows a metabolic acidosis with significantly increased lactate level and l/p quotient, and statistically distinct decrease of ph, oxygen tension, and bicarbonate level. 2. there is a considerable disproportion between the changes of acid-base metabolism, gas tension, and metabolic products in cerebrospinal fluid compared with much less expressed disturbances in the blood. 3. the advanced edematous phase s ...	1976	15353
intestinal myoelectric activity in response to live vibrio cholerae and cholera enterotoxin.	the myoelectric response of the rabbit ileum was studied in response to live vibrio cholerae culture, a whole cell lysate of cholera, and the purified enterotoxin. each cholera preparation produced a series of highly organized migrating action potential complexes (mapc). an mapc was defined as action potential discharge with a duration of 2.5 s or longer, followed by similar activity on at least one other consecutive electrode site. the mean and modal onset time of mapc activity occurred 4 h aft ...	1976	932212
in vitro production of ige by human peripheral blood lymphocytes: effect of cholera toxin and beta adrenergic stimulation.	peripheral blood lymphocytes from two human donors with elevated serum ige concentrations were maintained in short term tissue culture preparations. repeated culture preparations demonstrated that ige was produced in vitro in amounts that could be measured by the double antibody radioimmunoassay technique. the amount of ige produced by replicate cultures of cells from a single bleeding of the donor was similar when the cultures were simultaneously prepared. in contrast, ige production by the sam ...	1976	932435
suppression of growth of vibrio cholerae in the intestine of immunized mice.	in ligated intestinal loops of actively immunized adult mice, growth of v. cholerae 569b was suppressed approximately seven fold when compared to bacterial growth in non-immune animals. similarly, growth of v. cholerae 569b was reduced in mice immunized with a hybrid vibrio strain, ncv569b-165, which shares only flagella antigens with v. cholerae 569b. immunofluorescence studies of intestinal loop contents and intestinal sections indicated that, in non-immune mice, vibrios coated the intestinal ...	1976	934024
interaction of cholera toxin and ganglioside g(m1).	the exotoxin produced by vibrio cholerae is rapidly and firmly bound to the outer membrane of mammalian cells. with simple in vitro and in vivo methods and very pure gangliosides and allied glycolipids we have demonstrated that the monosialosylganglioside gm1 is the natural receptor for the cholera toxin. this ganglioside binds the toxin with a high affinity and inactivates it. the inactive derivative, choleragenoid toxoid has the same affinity to gm1 as the toxin. ganglioside gm1 was isolated f ...	1976	937147
the role of gangliosides in the action of cholera toxin.		1976	937148
stimulated flow of pancreatic and biliary secretions after intestinal exposure to cholera toxin.	1. duodeno-jejunal intestinal loops in rats were perfused with cholera toxin and the pancreatic and biliary secretory responses studied. 2. intraluminal cholera toxin induced a significant flow of pancreatic and biliary fluid and sustained protein (enzyme) secretion in pancreatic juice, in addition to the expected stimulation of mucosal secretion. 3. intravenously injected cholera toxin failed to elicit a secretory flow from the pancreas and biliary tract. 4. the time-course of the enhanced secr ...	1976	939069
[a new method of isolating pure cultures of the cholera vibrio. iii].		1976	941603
cholera toxin inhibits interferon action.		1976	942430
structure and specificity of gangliosides.		1976	945662
[choleragen and neuraminidase production by vibrio in vitro].	in vitro studies demonstrated a high degree of the rank correlation between the synthesis of cholera exotoxin and neuraminidase by cholera vibrios (v. cholerae 569b). the appearance of these biochemically-active materials proved to depend on the growth phase of the microbial population. a possibility of cooperation between them in the pathogenesis of cholera is suggested.	1976	947378
[model of a chronic vibrio cholerae carrier based on gnotobiotic rats].	germ-free monoflora (contaminated with nonpathogenic spore-bearing bacillus) and common albino rats (ofa) were infected with v. cholera el tor, of ogava and inaba serological types (6 milliard microbial cells per 1.5 ml of physiological solution per rat). approximately one week after the infection the number of vibrios reached hundreds of millions per 1 g of feces and persisted at this level for over 100 days (observation period). newborn rats were infected by natural way from adult vibrio carri ...	1976	947380
inactivation of the biological activities of the thermostable direct hemolysin of vibrio parahaemolyticus by ganglioside gt1.	biological activities of the thermostable direct hemolysin produced by vibrio parahaemolyticus, such as its hemolytic activity and lethal activity, were inhibited by neuraminidase-sensitive gangliosides, of which gt1 ganglioside was the most inhibitory. neuraminidase-resistant gangliosides did not affect the activities of the hemolysin. results showed that horse erythrocytes, which are resistant to the hemolysin, do not contain the neuraminidase-sensitive gangliosides gt1 and gd1a. therefore, we ...	1976	947841
uptake and metabolism of gangliosides in transformed mouse fibroblasts. relationship of ganglioside structure to choleragen response.	nctc 2071 cells, transformed mouse fibroblasts, when grown in chemically defined medium, are deficient in gangliosides and do not respond to choleragen. the cells lack two biosynthetic enzymes, cmp-sialic acid:lactosylceramide sialyltransferase and udp-galactose:gm2 (galnac-[acneu]-gal-glc-ceramide) galactosyltransferase, which are required for ganglioside synthesis. following uptake of ganglioside gm1 (gal-galnac-[acneu]-gal-glc-ceramide) from the medium, the cells respond to choleragen; howeve ...	1976	947892
[polyvalent diagnostic cholera phage].	a polyvalent diagnostic bacteriophage for rapid identification (in 6-10 hours) of cholera vibrio of both biotypes has been constructed of virulent e1 tor phages and recommended for laboratory practice. polyvalent diagnostic bacteriophage is strictly specific, inactive against the nonagglutinating vibrios, microbes of enteric group and those closely affiliated.	1976	948962
[study of the fractional makeup of diagnostic nutrient media for isolating vibrio cholerae].	a study was made of the fractional composition of peptones used for preparation of solid nutrient media. it was shown that the best growth of the vibrio on these media was obtained with the use of highly split peptones. examination of fractional composition of peptone by gel-filtration and fractionation with various precipitants is recommended for rational working out of nutrient media for cultivation of cholera vibrio.	1976	948963
effect of growth temperature and culture age on the lipid composition of vibrio cholerae 569b (inaba).	the relative amounts of the major phospholipids (phosphatidylethanolamine, phosphatidylglycerol and lyso-phosphatidylethanolamine) and fatty acids in vibrio cholerae 569b (inaba) varied with growth temperature and between exponential and stationary phases of growth.	1976	950557
[study of the ultrastructure of various representatives of the genus vibrio in relation to toxicogenesis].		1976	950927
a bioptic study of gastrointestinal mucosa in cholera patients during an epidemic in southern italy.	a histological biopsy study of gastric and jejunal mucosa of eight acute cholera patients during an epidemic in southern italy was carried out. the study demonstrated in all patients an intact epithelial lining of gastric and jejunal mucosa, a moderate degenerative process of enterocytes, presence of inflammatory lesions manifested by edema, vascular congestion, mononuclear cell infiltrate of lamina propria, and discharge of goblet-cells mucus. these changes reverted to normal in a few days. the ...	1976	952275
[the dehydrogenation of butandiol by "vibrio parahaemolyticus" and other vibrios (author's transl)].	the butandiol dehydrogenase was examined in 64 vibrios isolated in togo 1970/71. this enzyme was found in all of the not halophilic strains and in the species vibrio alginolyticus, whereas the species v. parahaemolyticus exhibited as well dehydrating as not dehydrating strains. the butandiol dehydrogenase could be a good marker for epidemiologic analysis in characterizing v. parahaemolyticus strains isolated from different sources, especially if no antisera are available.	1976	952445
a case of cholera in kingston, ont.	a case of cholera occurred in kingston, ont. in 1974 in a traveller from south africa. treatment, based on an understanding of the pathophysiology of cholera diarrhea and the mechanism of action of the vibrio cholerae enterotoxin on gastrointestinal fluid loss, consisted of correcting the severe loss of fluid and electrolytes and the metabolic acidosis, as soon as the patient could tolerate taking fluids orally, further fluid replacement consisted increasingly of oral administration of glucose a ...	1976	953912
laboratory investigation and infection control of cholera in kingston, ont.	vibrio cholerae, biotype el tor, was isolated in a hospital laboratory in kingston, ont. in 1974. confirmation and complete identification by the ontario regional and provincial public health laboratories was obtained within 3 days. institution of well established infection-control and public health measures prevented spread of the infection within the hospital and the community.	1976	953913
cholera: synopsis of clinical aspects and principles of treatment.	cholera varies greatly in clinical severity; the mortality of untreated severe cholera may be as high as 60% the main clinical feature is dehydration; fluid lost in the stools may amount to 60/. rehydration is the cornerstone of treatment. the amount of fluid required is approximately 10% of body weight in severe dehydration and 5 to 8% in moderate dehydration. fluid therapy, which must be individualized, may be successful on its own, but chemo-therapy shortens the duration of illness. tetracycl ...	1976	953914
bacterial proteinaceous products (bacteriocins) as cytotoxic agents of neoplasia.	several bacteriocins, bacterial proteinaceous antibiotics, are shown to markedly inhibit the division of various established (neoplastic) mammalian cell lines. the bacteriocins tested originated from escherichia coli, pseudomonas aeruginosa, vibrio cholerae, and vibrio eltor. using exponentially growing l60t mouse fibroblasts, the inhibitory effect was concentration dependent, and a growth inhibitory unit, equivalent to cytotoxic index 50, was established. expression of toxicity as a function of ...	1976	953984
effect of cholera toxin on intramembranous particles of a murine lymphoid cell line.		1976	954857
resistance of purified cholera toxin to enzymatic treatment with pancreatic elastase and papain.	treatment of cholera toxin with pancreatic elastase and papain in vitro showed a high resistance of the toxin molecule to these enzymes, under non-denaturing conditions or in the presence of 2 m urea. these experiments support the hypothesis of a particularly stable molecular structure of the toxin, as an explanation of its activity in the intestinal lumen where the pancreatic proteases are active.	1976	955031
possibilities of immunization against cholera and related enterotoxic enteropathies.	scientifically controlled field studies have established that parenterally administered killed vibrio vaccines or somatic antigen preparations offer only limited degrees of protection in certain population groups and have made it obvious that new approaches to the immunoprophylaxis of cholera are needed. it has now also been established that the symptoms of cholera result from the action of the cholera enterotoxin (choleragen) on the epithelial cells of the small intestine. immulogically relat ...	1976	955264
chemical composition & fatty acid pattern of lipopolysaccharides from vibrio cholerae.		1976	955660
role of mg2+ & fe3+ on the reversal of ni2+ toxicity in inaba serotypes of vibrio cholerae & vibrio eltor.		1976	955689
the effects of sodium ricinoleate on small intestinal function and structure.	the mechanism of hydroxy fatty acid-induced secretion was investigated in perfused hamster small intestine in vivo. sodium ricinoleate at an 8-mm concentration resulted in not only secretion of water and sodium, but an increase in intestinal clearance of inulin and a 16,000 mol wt dextran as well. a concentration of ricinoleate (2 mm) which did not affect water transport, however, did not alter intestinal permeability. ricinoleate-induced intestinal secretion was also accompanied by increased mu ...	1976	956372
cholera toxin-peroxidase: changes in surface labeling of glioblastoma cells with increased time in tissue culture.	cholera toxin coupled to peroxidase yielded a highly specific ultrastructural marker of plasma membrane monosialogangliosides. studies with cultures of brain and brain tumors suggested that long-term culture of tissue in monolayers results in eventual loss of surface monosialogangliosides.	1976	959817
cholera toxin-peroxidase: changes in surface labeling of glioblastoma cells with increased time in tissue culture.	cholera toxin coupled to peroxidase yielded a highly specific ultrastructural marker of plasma membrane monosialogangliosides. studies with cultures of brain and brain tumors suggested that long-term culture of tissue in monolayers results in eventual loss of surface monosialogangliosides.	1976	959818
[patterns in the body's immune response to administration of el tor vibrio cholera toxin to experimental animals].	a study was made of some regularities attending the mechanisms of antibody production in the organism of experimental animals after the administration of el tor cholera vibrio toxin. as revealed, the indices of the immune response depended on the chosen model, the method of its determination, the method of administration and the amount of the toxin administered. dynamics of the primary and secondary responses of the immunocompetent cells of mouse spleen was determined. with the action of the el ...	1976	961236
[identity of the h-antigen in cholera and nag vibrios].		1976	961255
[effect of cholerogen on tissue culture cells].	a study was made of various series of cholerogen on the cultures of human continuous cells of normal (f1, rh) and tumour nature (hela). cholerogen proved to produce a marked toxic action on the cultures of f1, rh and hela cells causing a reduction of the number of living cells, depression of mitotic activity, a reduction of the intensity of staining on the sum total protein and rna, a reduction of the activity in the cells of acid phosphatase and succinic dehydrogenase, and also a reduction of p ...	1976	961265
fluorometric assay of sialic acid in the picomole range: a modification of the thiobarbituric acid assay.		1976	962090
photoreactivating property of the vibrio cholerae cell system.	ultraviolet-inactivated chlora phage pl163/10 underwent a maximum of abot 36% of photoreactivation within the host v. cholerae cells. uv-inactivated but non-infected v. cholerae cells also underwent the same degree of photoreactivation.	1976	963629
demonstration of neuraminidase activity in human blood serum and human milk using a modified, radioactively labelled alpha1-glycoprotein as substrate.	n-acetylneuramini acid of alpha1-glycoprotein was oxidized with a small molar excess of periodate and reduced with tritium-labelled borohydride. by this method about 50% of the n-acetylneuraminic acid was converted to its radioactively labelled c8-analog and 25% to its c7-analog. using this modified alpha1-glycoprotein as substrate, minimum neuraminidase concentrations of 10(-18) units/ml, related to the activity of neuraminidase from vibrio cholerae, could be determined. neuraminidase activity ...	1976	964915
enhancement by sodium chloride of the selectivity of thiosulfate citrate bile salts sucrose agar for isolating vibrio cholerae biotype el tor.	in this study we utilized the salt-tolerant characteristics of vibrios to develop a more selective medium by addition of nacl to thiosulfate citrate bile salts sucrose (tcbs) agar. the effect of adding salt to tcbs agar varied greatly among brands of tcbs agar and between lots of the same brand. the addition of salt at concentrations as high as 1.5% (2.5% total nacl) caused the inhibition of growth of three species of commonly encountered normal bowel flora and one strain of classical vibrio cho ...	1976	965476
[epidemiological characteristics of cholera e1-tor].		1976	968590
[isolation and biological characterisitcs of l-forms of cholera vibrios].		1976	969878
effects of cholera toxin on cellular and paracellular sodium fluxes in rabbit ileum.	the diarrhea observed in patients with cholera is known to be related to secretion of water and electrolytes into the intestinal lumen. however, the exact mechanisms involved in these secretory processes have remained unclear. although it is clear that purified toxin acts on epithelial cell metabolism, its activity on na+ transport across intestinal mucosa is equivocal: reported either to prevent net na+ absorption or to cause net secretion of na+ from serosa to mucosa. since total transmural na ...	1976	971435
cell-mediated immunity to vibrio cholerae with ribonucleic acid-protein fractions of v. cholerae l-form lysates.	different l-form lysate vaccines of vibrio cholerae serotypes ogawa and inaba and their combination along with ethyl alcohol-precipitated ribonucleic acid (e-rna) and phenol-extracted rna (p-rna) fractions of v. cholerae ogawa lysates were tested for production of cell-mediated immunity. both e-rna and p-rna fractions induced an increase in leukocyte migration inhibition, macrophage migration inhibition, and macrophage aggregation. they also induced delayed hypersensitivity in rabbits. more cons ...	1976	971951
vibrio cholerae adherence and colonization in experimental cholera: electron microscopic studies.	colonization of the intestinal epithelium by vibrio cholerae was examined in two model systems, in ligated ileal loops of adult rabbits and in the patent gut of infant rabbits, using both scanning and transmission electron microscopy. time studies in the adult model showed a lag period of up to 1 h before the attachment of significant numbers of the vibrios. the bacteria appeared initially in small patches on the sides of the villi, predominantly along the transverse furrows. the number of adher ...	1976	971962
primary structure of the b subunit of cholera enterotoxin.		1976	976480
bacteriological studies of cholera in visakhapatnam region of andhra pradesh.		1976	977064
colonization of ascaris lumbricoides by v. cholerae.		1976	978375
immunotherapy and chemotherapy in children with neuroblastoma.	recent advances with immunotherapy in animal tumors suggested that trials with a combination of chemotherapy and immunotherapy in human malignant tumors might be worthwhile. a pilot program with vibrio cholera neuraminidase-treated tumor cells plus bcg was tested in 3 patients who had had chemotherapy for disseminated neuroblastoma. two of these children were in "complete remission" after radiation therapy and chemotherapy before the administration of immunotherapy. relapse occurred in 5-6 month ...	1976	978774
[non-agglutinable vibrios (nag-vibrios) and cholera (author's transl)].		1976	979518
[neuraminidase as a pathogenicity factor in microbial infections (proceedings)].		1976	983495
the cholera enterotoxin--robert koch revisited [proceedings].		1976	983499
activation by cholera toxin of adenylate cyclase solubilized from rat liver.	cholera toxin, or peptide a1 from the toxin, activates adenylate cyclase solubilized from rat liver with lubrol px, provided that cell sap, nad+, atp and thiol-group-containing compounds are present. the activation is abolished by antisera to whole toxin, but not to subunit b.	1976	985419
adhesive properties of vibrio cholerae: adhesion to isolated rabbit brush border membranes and hemagglutinating activity.	adhesion of vibrios to the small intestine may occur (i) by association of the bacteria with secreted mucus gel or (ii) by adherence of the bacteria to the surface of epithelial cells. in the present study, vibrios readily adhered to isolated brush border membranes obtained from rabbit intestinal epithelial cells. adhesion was temperature dependent and required the presence of divalent cations such as calcium. the agglutination of human o erythrocytes by vibrio cholerae was observed also, and th ...	1976	985804
adhesive properties of vibrio cholerae: nature of the interaction with isolated rabbit brush border membranes and human erythrocytes.	nonmotile vibrio mutants lacked the ability to adhere to rabbit intestinal brush border membranes and to agglutinate human group o erythrocytes, but motile revertant vibrios isolated from such strains expressed adhesiveness equivalent to that of the original parent. two possible explanations for the relation between vibrio motility and adhesion in these assays systems are (i) that the rate of adhesion depends on the rate of chance contact brought about by motility, and (ii) that the flagellum ei ...	1976	985805
biochemistry of vibrio cholerae virulence: purification of cholera enterotoxin by preparative disc electrophoresis.	procedures for cholera enterotoxin purification previously developed in this labarotory were not applicable to large-scale purification, and these methods resulted in low yields of pure toxin. an efficient scheme has been developed whereby pure cholera enterotoxin can be obtained from 6 to 8 liters of culture supernatant fluid. this method consists of concentration by membrane ultrafiltration followed by gel filtration and cation-exchange chromatography. pure cholera enterotoxin of high biologic ...	1976	987751
practical estimation of the actual laboratory diagnostics of cholera.		1976	990543
active and passive immunity to cholera toxin.		1976	992728
determination of molecular weight of vibrio cholerae genome dna from renaturation rate.		1976	992812
experimental development of antibiotic resistance in vibrio cholerae.		1976	992839
immunological control mechanism against cholera toxin: interference with toxin binding to intestinal receptors.	the immunological control mechanism against cholera toxin (ct) in the small intestine of rats was studied in vivo. ct binding to intestinal receptors was determined by injected radiolabeled ct into the loops of rat small intestine and subsequently separating purified microvillus membranes from mucosal scrapings of those loops. substantial radioactivity (10(5) cpm/mg of microvillus protein) was present in microvillus fractions of small intestine exposed to 125i-labeled ct compared to radioactivit ...	1976	992868
an investigation of alternatives to hog gastric mucin as virulence-enhancing agents in the cholera vaccine potency assay.		1976	993220
preventing insect diapause with hormones and cholera toxin.		1976	994697
[sensitivity to antibiotics of el tor cholera vibrions].		1976	999265
the immunologic control mechanism against cholera toxin. ii. stimulation of adenylate cyclase without fluid secretion.		1976	999681
cholera toxin and adenylate cyclase: properties of the activated enzyme in liver plasma membranes.	adenylate cyclase (ec 4.6.1.1) activity in mouse liver plasma membranes is increased fivefold when animals are pretreated with cholera toxin. the increase in activity is detectable within 20 min of an intravenous injection of the toxin. the response of the control and cholera-toxin-activated adenylate cyclase to hormones, gtp, and naf is complex. gtp causes the same fold stimulation of control and toxin-activated cyclase, but glucagon and naf remain the most potent activators of liver adenylate ...	1976	1000362
[case of contamination of the karst underground mineral-water stratum with vibrio cholerae].		1976	1001931
studies of the ligand binding to cholera toxin, i. the lipophilic moiety of sialoglycolipids.	the fixation of cholera toxin by ganglioside ggtet1 is dependent on the nature of the carbohydrate as well as the lipid moiety of the glycolipid. the role of the lipid in binding to the toxin investigated with synthetic ganglioside analogues (gangliosidoides). the interaction between glycolipid and toxin was followed by precipitate formation, by inhibition of toxicity and in polyacrylamide gel electrophoresis. for specific precipitation, an aliphatic hydrocarbon chain at least 14 c-atoms in leng ...	1976	1002130