Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| differential effects of prostaglandin synthetase inhibitors on prostaglandin e2 binding and on prostaglandin- or cholera toxin-induced cyclic amp accumulation in the rabbit uterus. | cyclic 3',5'-nucleotide phosphodiesterase (pde) activity in rabbit uterine homogenate was inhibited by indomethacin (10 mug/ml; 66% inhibition) or flufenamic and (10 mug/ml; 60%). indomethacin (100 mug/ml) reduced uterine prostaglandin e2 (pge2) content by 80%, but potentiated the stimulatory action of purified cholera toxin (choleragen; 800%) and of exogenous pge2 (140%) on cyclic amp accumulation, probably through its inhibitory effect on cyclic amp destruction. these findings suggest that end ... | 1976 | 187046 |
| [subcspsular blastema of the adrenal cortex of the swine (sus scrofula domestica) after continuous acth long-term application]. | 1976 | 187085 | |
| transfer of parental immunity to infectious laryngotracheitis in chicks. | the transfer of parental immunity to infectious laryngotracheitis was appraised by measuring serum antibody levels in 150 chicks from the day of hatch up to five weeks. the breeder flock which had received primary vaccination at eight weeks and a booster at 20 weeks transferred high antibody levels which fell markedly within two weeks and remained constant thereafter. chicks whose parents were vaccinated at 20 weeks only, had low antibody levels throughout. these low levels, in either group of c ... | 1976 | 187298 |
| [symptomatology of diseases caused by c1. perfringens]. | 1976 | 178949 | |
| [antibodies to viral antigens in rheumatoid arthritis]. | 1976 | 179153 | |
| viral diseases of cats: current concepts. | 1976 | 179185 | |
| [the diuretic action of morphine in rats]. | 1976 | 179647 | |
| [role of bioenergetic changes in the mechanism of action of nonsteroid anti-inflammatory agents]. | in experiments on rats the antiphlogistic action of sodium mephenaminate and salicylate was found to be more pronounced under conditions of ovalbumin than that of dextran inflammation. the antiphlogistic effect of sodium salicylate was greater than that of mephenaminate in ovalbumin inflammation; this correlated with a more marked drop in the content of lactic acid in the blood and with a more complete elimination of uncoupling of oxidative phosphorylation in the hepatic mitochondria than in cas ... | 1976 | 179649 |
| arginyl-trna synthetase from baker's yeast. purification and some properties. | arginyl-trna synthetase from baker's yeast (saccharomyces cerevisiae, strain 836) was obtained pure by a large-scale preparative method, which involves four chromatographic columns and one preparative polyacrylamide gel electrophoretic step. the enzyme has a high specific activity (9000 u/mg) and consists of a single polypeptide chain of molecular weight approximately 73000 as determined by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate. amino acid analysis of the e ... | 1976 | 179818 |
| biochemical and mechanical effects of phenylephrine on the heart. | injections of phenylephrine into isolated perfused guinea pig hearts increased cyclic amp and phosphorylase a. reserpine pretreatment or propranolol decreased or abolished the phenylephrine-induced biochemical changes without affecting the contractile response. phentolamine, on the other hand, shifted the phenylephrine dose-response curve for contractility to the right without affecting the other parameters. the biochemical effects of phenylephrine are apparently due to the release of noradrenal ... | 1976 | 179825 |
| [radioreceptor assay of gonadotropin]. | 1976 | 180307 | |
| sodium-stimulated alpha-aminoisobutyric acid transport by membrane vesicles from simian virus-transformed mouse cells. | uptake of alpha-aminoisobutyric acid, by membrane vesicles derived principally from the plasma membrane and endoplasmic reticulum of mouse 3t3 cells transformed by simian virus 40, is stimulated by sodium chloride. both in the presence and absence of na+ uptake is time-dependent and osmotically sensitive. the na+-stimulated uptake is inhibited by other amino acids. the kinetics of transport of alpha-aminoisobutyric acid are shown to be biphasic both in whole cells and in the membrane vesicles. o ... | 1976 | 180527 |
| [characteristics and function of cholera toxin molecule (author's transl)]. | 1976 | 194285 | |
| intercellular immunological controls and modulation of cyclic amp levels. some doubts. | we have re-examined two sets of observations put forward to support the hypothesis that rises in camp levels induced by vasoactive amines and prostaglandins are involved in the intercellular control of immunological and inflammatory processes. (1) this hypothesis is said to be supported by the fact that cholera toxin, which raises camp levels in lymphoid tissue in vivo, is immunosuppressive. however, we found that cholera toxin inhibited antibody production only if given in doses causing gross d ... | 1976 | 194828 |
| alteration in macromolecular glycosylation of transformed cells mediated by cholera toxin and dibutyryl adenosine 3':5'-cyclic monophosphate. | 1976 | 191314 | |
| pancytopenia and "caillary leak syndrome" with infectious mononucleosis. | a 19-year-old man with documented infectious mononucleosis presented with pancytopenia and a megaloblastic bone marrow. he developed a "capillary leak syndrome" with an expanded plasma volume of 9,290 ml and normal right heart and pulmonary artery pressures. the patient had a dramatic recovery after corticosteroid therapy. | 1976 | 175508 |
| positive inotropic activity of cholera enterotoxin on the embryonic chick heart. | the characteristics of the positive inotropic effect of different preparations of cholera toxin on the isolated 4-day-old embryonic chick heart were investigated. crude (cct) as well as partially purified and purified (pct)preparations of cholera toxin were shown to have positive inotropic activity. contractility was increased within 1 minute and reached a maximum at approximately 40 minutes after exposure to toxin. activities of all three toxin preparations were abolished by heating, their effe ... | 1976 | 177757 |
| functional incorporation of ganglioside into intact cells: induction of choleragen responsiveness. | nctc 2071 cells are unable to synthesize the monosialoganglioside gm1. when grown in chemically defined medium these cells contained no detectable gm1 and did not accumulate 3': 5'-cyclic amp in response to choleragen. incubation of the cells with [3h]gm1 permitted quantification of ganglioside uptake which was dependent on time and concentration of [3h]gm1 in the medium. responsiveness to choleragen was demonstrated with binding of as few as 17,000 molecules of [3h]gm1 per cell; a maximal respo ... | 1976 | 177969 |
| pregnancy interrupting effects of some bacterial toxins. | embryotoxic properties of shigella dysenteriae and clostridium perfringens toxins, of e. coli endotoxin, v. cholerase and e. coli enterotoxins were compared in mice. e. coli endotoxin has embryotoxic effects at all stages of pregnancy. e. coli enterotoxin v. cholerae enterotoxin and shigella dysenteriae toxin are most effective mainly at earlier stages of pregnancy. clostridium perfringens toxin has no embryotoxic effect. | 1976 | 178014 |
| monolayer cultures of normal adult rat adrenocortical cells: steroidogenic responses to nucleotides, bacterial toxins and antimicrotubular agents. | monolayer cultures of normal rat adrenocortical cells were treated with agents which stimulate steroidogenesis by y-1 adrenal tumour cells. choleragen was active, whereas cyclic nucleotides other than cyclic amp, bacterial endotoxins and antimicrotubular agents were inactive. | 1976 | 178525 |
| activation of intestinal mucosal adenylate cyclase by shigella dysenteriae i enterotoxin. | because the mechanism whereby shigella dysenteriae i enterotoxin induces intestinal secretion is unclear, the effect of this toxin on adenylate cyclase activity in rabbit ileal mucosa was studied under various in vitro and in vivo conditions. activation of adenylate cyclase by shigella enterotoxin was observed only when substrate (atp) concentrations above the km of adenylate cyclase were employed. these concentrations of atp are greater than those required to demonstrate activation of adenylate ... | 1976 | 178569 |
| effects of cholera enterotoxin, glucagon, and dibutyryl cyclic amp on rat liver alkaline phosphatase, bile flow, and bile composition. | cholera enterotoxin, 45 mug per 250 g body weight, administered intravenously to rats, caused a 6-fold rise in the activity of liver alkaline phosphatase in 12 hr. there was no change in bile volume or in the concentration or total bile content of na+, k+, hco3-, or cl- for 36 hr after the administration of cholera toxin. however, bile phospholipid output fell markedly from a control level of 15.0 +/- 1.0 mumol per 6 hr to a low level of 4.0 +/- 1.2 mumol per 6 hr in the 12- to 18-hr collection, ... | 1976 | 176082 |
| immune host response to corneal grafts sensitized to herpes simplex virus. | experiments were performed to study the fate of corneal grafts sensitized to herpes antigens when grafted to hsv-sensitized hosts. nonsensitized grafts in systemically sensitized hosts remained clear, whereas all locally sensitized grafts in systemically sensitized hosts became opaque within ten days. the stroma showed severe lymphocytic and plasma cell infiltration and neovascularization. one-half of the corneas from systemically sensitized hosts grafted to eyes previously infected opacified in ... | 1976 | 176129 |
| activation of adenylate cyclase by cholera toxin in rat liver homogenates. | the effect of cholera toxin on adenylate cyclase from rat liver has been studied in a broken cell preparation. the activation of the enzyme in this in vitro preparation requires the addition of nicotinamide adenine dinucleotide (nad) to the incubation medium and the presence of cell components other than the membrane-bound adenylate cyclase. once the activation of the cyclase is produced, the effect persists despite repeated washing or solubilization of the enzyme. the effect can be obtained wit ... | 1976 | 176181 |
| mechanism of activation adenylate cyclase in vitro by polymyxin-released, heat-labile enterotoxin of escherichia coli. | heat-labile enterotoxic material released from escherichia coli by polymyxin b activates the adenylate cyclase of pigeon erythrocyte ghosts in a time- and concentration-dependent manner. the activation requires nicotinamide adenine dinucleotide, adenosine triphosphate, and another component of the erythrocyte supernatant. the active species has a molecular weight of about 23,000-24,000 daltons, is inhibited by antibodies to the toxin of vibrio cholerae, and is not irreversibly denatured by sodiu ... | 1976 | 176279 |
| mode of action of vibrio cholerae enterotoxin in cultured adrenal tumor cells. | the effects of vibrio cholerae enterotoxin on steroidogenesis and on formation of adenosine 3':5'-cyclic phosphate (cyclic amp) in two adrenal tumor cell lines were compared. steroidogenesis was half-maximal at concentrations of 1 ng of cholera toxin/ml in the mutant os-3 cells and 3 ng of cholera toxin/ml in the parent y-1 cells. at the end of an 8-hr incubation, toxin-induced formation of cyclic amp in the mutant cell line was reduced by 90%. a molar ratio of gm1 ganglioside (galactosyl-n-acet ... | 1976 | 176280 |
| multiple roles of erythrocyte supernatant in the activation of adenylate cyclase by vibrio cholerae toxin in vitro. | peptide a1 of vibrio cholerae toxin, nicotinamide adenine dinucleotide, adenosine triphosphate, and a soluble protein present in erythrocyte supernatant are required for the activation of pigeon erythrocyte ghost adenylate cyclase but are not required to maintain the activated state. the compounds are all required simultaneously, and when all are added to ghosts, adenylate cyclase activity increases at a linear rate without delay. under optimal conditions significant activation of cyclase is giv ... | 1976 | 176281 |
| cholera toxin and the adenylate cyclase-activating signal. | studies with chemically modified cholera toxin derivatives showed that all treatments that decreased the ability of toxin to bind to mouse thymus cells or to polystyrene-coupled gm1 ganglioside caused a concomitant reduction in the toxin's ability to increase adenosine 3':5'-cyclic phosphate (cyclic amp) in thymus cells and skin vascular permeability in rabbits. dissociation of the h (heavy) and l (light) subunits abolished the biologic activity without inhibiting receptor binding, as did treatm ... | 1976 | 176282 |
| interaction of vibrio cholerae toxin with sarcoma 180 cell membranes. | three discrete phases are discernible in the activation, by vibrio cholerae toxin, of adenylate cyclase in fragments of sarcoma 180 cell membranes. in the first, or preparatory, phase the toxin must be exposed to dithiothreitol or nicotinamide adenine dinucleotide (nad) in the absence of the membranes. in the second phase, the prepared toxin is dissociated to yield a macromolecular cyclase-activating factor (mcaf) in the presence of the membranes. in the third phase, membrane basal adenylate cyc ... | 1976 | 176283 |
| orphan airlift. enteric pathogens isolated from vietnamese children immigrating to the united states. | isolation studies for bacterial and parasitic agents were carried out on stool specimens from vietnamese infants at the time of their mass airlift to the united states. one or more bacterial pathogens were found in 49% of the 367 stool specimens cultured. the isolates included enteropathogenic escherichia coli (161), shigella (16), salmonella (15), but no salmonella typhi or vibrio cholerae. parasites identified in 88 stool specimens included giardia lamblia (10), ascaris lumbricoides (7), and e ... | 1976 | 176481 |
| inhibition of enterotoxin-induced intestinal secretion by the polypeptide antibiotic, polymyxin. | 1976 | 176498 | |
| structure and function of cholera toxin and hormone receptors. | the enterotoxin from vibrio cholerae is a protein of 100,000 mol wt which stimulates adenylate cyclase activity ubiquitously. the binding of biologically active 125i-labeled choleragen to cell membranes is of extraordinary affinity and specificity. the binding may be restricted to membrane-bound ganglioside gm1. this ganglioside can be inserted into membranes from exogenous sources, and the increased toxin binding in such cells can be reflected by an increased sensitivity to the biological effec ... | 1976 | 176537 |
| [the effect of multiple injections of acth (alone and combined with sodium ribonucleinate) on incorporation of p32-orthophosphate into the mitochondrial rna of the liver, spleen and heart of white rats]. | 1976 | 176651 | |
| nitrogenase of azotobacter chroococcum. kinetics of the reduction of oxidized iron-protein by sodium dithionite. | the kinetics of the reduction of oxidized fe-protein of nitrogenase from azotobacter chroococcum by sodium dithionite were studied by stopped-flow and rapid-freezing e.p.r. (electron-paramagnetic-resonance) spectroscopy. the appearance of the gav. = 1.94 e.p.r. signal (0.24 electron integrated intensity/mol) was associated with a one-electron reduction by so2--with k greater than 10(8)m-1-s-1 at 23 degrees c. a value of k = 1.75s-1 was obtained for the rate of dissociation of s2o42- into 2so2-- ... | 1976 | 180978 |
| endonucleolytic cleavage of murine leukemia virus 35s rna by microsome-associated nuclease. | 1976 | 180996 | |
| phosphorylation of the active, a1 component of cholera toxin by protein kinase. | cholera toxin, an activator of adenylate cyclase in a wide variety of cells, is a substrate for the phosphotransferase reaction catalyzed by purified cyclic adenosine 5'-monophosphate dependent bovine cardiac muscle protein kinase and the protein associated with human erythrocyte membranes. phosphorylation occurs when the toxin is dissociated with 5-20 mm dithiothreitol and is restricted to the a1 or "adenylate cyclase activating" subunit of the toxin. | 1976 | 181050 |
| in vitro anamnestic antibody response: differential cellular and calcium requirements for induction by antigen and regulation by cyclic amp. | 1976 | 181149 | |
| characteristics of immunity induced by neuraminidase-treated lymphosarcoma cells in c3h (mtv+) and c3h (mtv-) mice. | the immunogenicity of 6c3hed lymphosarcoma ascites tumor cells was greatly enhanced upon treatment with vibrio cholerae neuraminidase. as a result, mammary tumor virus (mtv)-free c3h mice which had been repeatedly immunized with the treated cells resisted a challenge of 10(6) untreated viable cells inoculated i.p., whereas all control animals died after receiving less than 10 lymphosarcoma tumor cells. in contrast, there was no increase in the refractoriness to challenge when mtv-infected c3h mi ... | 1976 | 181351 |
| interferon action: role of membrane gangliosides. | 1976 | 181911 | |
| trophoblastic pseudotumor of the uterus: an exaggerated form of "syncytial endometritis" simulating a malignant tumor. | twelve cases of a hitherto unrecognized pseudotumorous trophoblastic invasion of the myometrium are analyzed. human chorionic gonadotropin (hcg) was identified by an immunoenzyme technique in the cytoplasm of the invasive cells. the lesion may be localized and only superficially invasive or deeply invasive and have a gross appearance suggesting a neoplasm in the excised uterus. in either case, this process has been confused with various types of malignant tumors, most often choriocarcinoma, from ... | 1976 | 182351 |
| the effects of cholera toxin on the adrenal weight in hypophysectomized rats. | the effects of cholera on adrenal weight in hypophysectomized rats were investigated, in an attempt to demonstrate an acth-like, adrenal trophic effect of the toxin. the results suggested that the toxin probably exerts is acth-like action on the adrenal via adenylate cyclase. cholera toxin was also shown to have a thermolytic action, similar to that of acth, probably due to stimulation of adrenal glucocorticoid secretion. | 1976 | 182572 |
| similarity of responses of cultured sertoli cells to cholera toxin and fsh. | the effects of cholera toxin on the responses of cultured sertoli cells were compared with those elicited by follicle-stimulating hormone (fsh), and n6o2'-dibutyryl-3',5'-cyclic amp (bu2camp). addition of fsh or cholera toxin increased camp levels. subsequently there was greater rates of conversion of testosterone to 17beta-estradiol, formation of androgen-binding protein (abp), and incorporation of [3h]thymidine into dna by sertoli cells prepared from testes of immature rats and cultured in the ... | 1976 | 182580 |
| stimulatory and inhibitory effects of cyclic amp on pancreatic glucagon release from monolayer cultures and the controlling role of calcium. | when glucagon release from monolayer cultures of newborn rat pancreas was measured over four hours in media containing 2.5 mm ca++, a significant cyclic amp-related inhibition of release was observed. this was noted whether intracellular cyclic amp levels were raised by the addition of exogenous cyclic amp or dibutyryl cyclic amp, by phosphodiesterase inhibition with theophylline, or by the stimulation of adenylate cyclase with cholera toxin. the inhibition was concentration dependent for cyclic ... | 1976 | 182608 |
| augmented immunogenicity of tumor cell membranes produced by surface budding viruses: parameters of optimal immunization. | membranes prepared from tumor cells infected with surface budding viruses are much more immunogenic than membranes from uninfected tumor cells. factors affecting immunization with membranes from virus-infected tumor cells were studied. preparations made with influenza virus were clearly superior to those prepared with vesicular stomatitis virus (vsv). membranes infected with vsv were maximally immunogenic at a dose equivalent to a 10% cell pack whereas influenza-virus-infected membranes were imm ... | 1976 | 182646 |
| inflammatory cells in solid murine neoplasms. i. tumor disaggregation and identification of constituent inflammatory cells. | mechanical and enzymatic methods of disaggregating tumors were studied with the goals of (1) minimizing cell losses while (2) maintaining functional and surface membrane markers needed to objectively identify inflammatory cells (ic)1 in resultant suspensions. application of the principles and methods described makes accurate estimation of the percentage of each ic type present in neoplasms possible for the first time. compared to purely mechanical means of disaggregating tumors, all enzyme mixtu ... | 1976 | 182647 |
| comparison of jc and bk human papovaviruses with simian virus 40: dna homology studies. | studies were performed to ascertain the relationship of human papovavirus jc to bk virus and to simian virus 40 (sv40) by further restriction endonuclease analysis and by dna-dna competition hybridization on membrane filters. form i dna extracted from two new isolates from cases of progressive multifocal leukoencephalopathy of human papovaviruses that were jc-like in their antigenic properties were found to yield restriction endonuclease fragmentation patterns similar to those of prototypic jc v ... | 1976 | 183019 |
| redistribution of immunoglobulin receptors on human neutrophils and its relationship to the release of lysosomal enzymes;. | release of the lysosomal enzyme beta-glucuronidase from human neutrophils was induced by igg or its fc fragment, aggregated by immune precipitation or by coating on latex particles. such release was inhibited when the cells were preincubated with free igg or fc fragments; f(ab')2 fragments were ineffective in both inducing and inhibiting beta-glucuronidase release. neutrophils incubated with igg or fc fragments, when challenged with anti-igg antibody, released lysosomal enzymes without the relea ... | 1976 | 183051 |
| increase of glucagon receptors in hyperthyroidism. | 1976 | 183145 | |
| cyclic amp and growth of ehrlich ascites tumor cells. lack of cyclic amp elevation in nutritionally deprived cells and mechanism of retardation of growth by dibutryl cyclic amp. | cyclic amp levels in ehrlich ascites tumor cells changed little after deprivation of cells of essential nutrients, serum, glucose and amino acids, deprival of each of which leads to marked inhibition of growth and protein synthesis. cyclic amp levels also changed little after the addition of these nutrients to deprived cells. thus cyclic amp is not likely to be the intracellular mediator for growth regulation by these three nutrients. elevation of cyclic amp levels for short periods by exposure ... | 1976 | 183828 |
| [adenylate cyclase system of the liver]. | 1976 | 183838 | |
| cytomegalovirus hepatitis in an artificial kidney unit. | serum hepatitis is a dreaded risk in connection with regular dialysis treatment (rdt). liver damage, however, can be cuased by other diseases, such as infection with cytomegalovirus (cmv). two cases in our artificial kidney unit revealed signs of liver damage with increased liver enzyme activity. case 1, a woman, was on rdt after an unsuccessful renal transplantation, and case 2, a man, belonged to the staff. serum hepatitis was initially suspected in both cases, but repeated examinations of the ... | 1976 | 184060 |
| effect of disinfectants on variola virus in cell culture. | twenty kinds of disinfectants were examined for ability to inactivate variola virus. cytopathic effect and plaque formation on monolayer cultures of an established monkey kidney cell line were used as indicators of virus inactivation. a micromethod using microplate cultures, and not requiring a co2 incubator, was adopted. the procedures were straightforward, showing good reproducibility. among the compounds tested, several were found to be superior because of the minimum concentrations required ... | 1976 | 184735 |
| surface antigens on hela cells persistently infected with hvj (sendai virus). | surface antigens of helahvj cells, a cell line persistently infected with hvj, were studied by fluorescent antibody staining. after absorption with concentrated hvj virions and hela cells, anti-helahvj antiserum was able to demonstrate specific surface fluorescence on helahvj cells, while this serum no longer reacted with original hela cells nor with hvj virions. during cytolytic infection of hela cells with hvj, this specific surface antigen appeared at an early stage of infection prior to the ... | 1976 | 184762 |
| a cyclic adenosine 3':5'-monophosphate-mediated effect of cholera toxin on high-molecular-weight glycoprotein species of malignant cells. | a comparison of the pronase-sensitive glycosylated species detectable under permissive and nonpermissive conditions by normal rat kidney cells transformed by a temperature-sensitive derivative of rous sarcoma virus reveals relative decreased labeling of high-molecular-weight glycosylated species under conditions that allow the expression of transformation, in medium supplemented either with 0.5% calf serum or with human alpha2-macroglobulin, 100 mug/ml. exposure of the cultures to cholera toxin ... | 1976 | 184943 |
| msh stimulates adenylate cyclase and tyrosinase in cultivated melanoma cells in the presence of cytochalasin b. | 1976 | 185075 | |
| the syrian hamster: a reproducible model for studying changes in intestinal fluid secretion in response to enterotoxin challenge. | syrian hamsters respond in a predictable and reproducible manner to intragastric administration of purified cholera enterotoxin by intraluminal accumulation of fluid in the small bowel, cecum, and proximal colon. in the majority of animals this process is self limiting, and recovery occurs with full reabsorption of intestinal fluid by 30 to 35 h. the secretory response to 75 mug of cholera toxin has been defined, and the model was utilized to study the inhibitory effects of indomethacin, polymyx ... | 1976 | 185149 |
| biosynthesis and function of gangliosides. | gangliosides are unique acidic glycolipids that are selectively concentrated in the plasma membrane of cells. surface labeling studies have demonstrated that at least a portion of the oligosaccharde chain of gangliosides extends beyond the hydrophe) is imbedded in the membrane bilayer. it is becoming increasingly apparent that gangliosides participate in the internalization of environmental signals elicited by cholera toxin and glycoprotein hormones such as thyrotropic hormone and chorionic gona ... | 1976 | 185697 |
| stimulation of adenylate cyclase in washed pigeon erythrocyte membrane with cholera toxin and its subunits. | 1976 | 185963 | |
| subunit structure of cyclic gmp-dependent protein kinase from guinea pig fetal lung: dissociation of holoenzyme by cyclic gmp and histone. | 1976 | 186069 | |
| [effect of propranolol and trimepranol on the activity of carboxylic esterase, acid and alkaline phosphomonoesterases and adenosinetriphosphatase in the human thyroid gland]. | 1976 | 186198 | |
| the activation of adenylate cyclase by cholera toxin: possible interaction with the nucleotide regulatory site. | the application of cholera toxin to intact cells causes a stimulation of adenylate cyclase activity. the effect is characterized by a lag period followed by a progressive rise in enzyme activity over several hours. only a few minutes' exposure to the toxin is required to produce effects lasting over several days. stimulation of adenylate cyclase by cholera toxin in broken cell preparations requires the presence of nicotinamide-adenine dinucleotide (nad) and an unidentified component of the cytos ... | 1976 | 186240 |
| adenylate cyclase in foetal rat testes and its stimulation by lh and naf. | 1976 | 186308 | |
| cyclic amp control by prostaglandin e1 in non-parenchymal liver cells. | 1976 | 186314 | |
| effect of estrogen on gene expression in the chick oviduct. correlation between nuclear-bound estrogen receptor and chromatin initiation site for transcription. | the [3h]estradiol exchange assay was used to characterize the nuclear estrogen receptor from the chick oviduct. after diethylstilibestrol (des) treatment (14 days), the oviduct nuclei contained estrogen receptors that manifested high affinity (kd = 2 x 10(-9)m) and low capacity binding (4000 to 5000 sites/cell) for estradiol. des and estradiol competed significantly for [3h]estradiol binding, while testosterone and progesterone were ineffective. these binding sites were found in the oviduct and ... | 1976 | 173720 |
| antibody-dependent cell-mediated cytotoxicity to target cells infected with type 1 and type 2 herpes simplex virus. | the phenomenon of antibody-dependent cell-mediated cytoxicity (adcc) has been extended to include target cells acutely infected with herpes simplex type 1 virus (hsv-1) or herpes simplex type 2 virus (hsv-2) in an in vitro system that employs immune human serum and human blood mononuclear cells. the cytotoxic reaction was detectable after 1 hr of incubation and was complete between 4 and 8 hr. the amount of adcc noted was directly proportional to the logarithm(10) of the effector: target cell ra ... | 1976 | 173757 |
| effects of chronic uremia, hemodialysis, and renal transplantation on plasma lipids and lipoproteins in man. | since quantitative and qualitative alterations in plasma lipoproteins may provide insights into mechanism(s) of altered lipid transport in renal failure, whole plasma triglyceride (tg) and cholesterol (chol) concentrations and lipoprotein neutral lipids and composition were examined in patients with chronic renal failure (undialyzed and dialyzed) and following successful renal transplantation. both uremic groups demonstrated increased tg (p less than 0.001) and normal chol in whole plasma and in ... | 1976 | 173766 |
| activation of renal erythropoietic factor by phosphorylation. | renal erythropoietic factor (ref) serves as a substrate for a cyclic nucleotide-dependent protein kinase. the phosphorylation of ref is associated with increased erythropoietic activity. conversely, ref isolated from hypoxic rats is a poor phosphate acceptor. these findings and the presence of a positive correlation between erythropoietin levels and urinary excretion of adenosine 3',5' monophosphate (cyclic-amp) in anemic individuals suggest that the cyclic-amp-protein kinase system plays an imp ... | 1976 | 173768 |
| interaction of ultrasound with neoplastic tissue. ii. systemic effects after local sonic irradiation. | local sonic irradiation was applied to subcutaneously implanted furth-columbia rat wilms' tumor. the weight and the rate of tritiated thymidine uptake were measured in host organs distal to the application field. kidney and spleen weights were inhibited by the wilms' tumor, and sonication of the tumor removed all or part of this inhibition. liver weight was increased after sonication of tumor-bearing rats but not in nontumor-bearing rats. this may have been a response to tumor-specific substance ... | 1976 | 174264 |
| association of herpes simplex virus type 1 dna with host chromosomal dna during productive infection. | 1976 | 174293 | |
| increased cyclic adenosine 3',5'-monophosphate content in guinea pig ileum after exposure to staphylococcus aureus delta-toxin. | to compare staphylococcus aureus delta-toxin with cholera toxin, which is known to increase cellular cyclic adenosine 3',5'-monophosphate (camp), studies were undertaken to determine the effect of delta-toxin on the camp content of guinea pig ileum maintained in vitro. concentrations of delta-toxin as low as 0.40 mug/ml increased camp levels in guinea pig ileum after 2 h of incubation. histological damage was seen in ileum exposed for 2 h to delta-toxin concentrations of 100 mug/ml. as little as ... | 1976 | 175015 |
| immunological properties of bovine serum lipoproteins and chemical analysis of their protein moieties. | four classes of bovine serum lipoproteins were isolated by precipitation with dextran sulfate, ultracentrifugation, and preparative electrophoresis on polyacrylamide gel. very low density lipoprotein (d less than 1.019 g/ml) was related immunologically to low density lipoprotein-two (d 1.039 to 1.060 g/ml) and high density lipoprotein (d 1.060 to 1.210 g/ml) was related immunologically to low density lipoprotein-one (d 1.019 to 1.039 g/ml), but the two pairs were immunologically distinct. the ma ... | 1976 | 175102 |
| ultrastructure of mycoplasma-like organisms purified from clover phyllody-affected plants. | 1976 | 175171 | |
| translation of oncogenic virus rna in xenopus laevis oocytes. | 1976 | 175294 | |
| [age dependent frequency of cytoplasmic antibodies]. | in a random sample of so-called healthy blood donors under and over 60 years of age (n=196 of 10,593 donations by about 4254 donors) the frequency of antibodies (antibodies against smooth muscles, mitochondria, microsomes, brush border of proximal rat renal tubuli, connective tissue, and parietal cells) detectable by immuno-fluorescence was determined and compared with the incidence thereof in various groups of diseases. in general, two frequency peaks (up to 30 and over 50 years of age) were ob ... | 1976 | 139091 |
| biochemical actions of sympathomimetic drugs which overcome cycloheximide-induced amnesia. | earlier investigations of sympathomimetic drugs overcoming the amnesic action of cycloheximide (cxm) in day-old chickens were extended to biochemical studies in vitro. the effects of amphetamine, norepinephrine, alpha and beta noradrenergic stimulants and receptor blockers on na+/k+ atp'ase activity in total homogenate of chicken forebrain were investigated. norepinephrine and the beta stimulant, isoprenaline significantly stimulated the activity of this enzyme, while the beta blocker, propranol ... | 1976 | 138858 |
| cryopreservation of lymphocytes for use in in vitro assays of cellular immunity. | human lymphocytes can be cryopreserved for the purpose of performing in vitro tests of cellular immunity. a systematic study of the conditions for freezing and recovering cells has shown that there is wide flexibility in cell concentrations of cryoprotective agent and the temperature and rate of dilution however, have definable optima. cells frozen and thawed under optimal conditions retain their reactivity in mlc and can be used for sequential studies of immune responsiveness. approximately 70% ... | 1976 | 130427 |
| dynamics of sulfonylurea-induced insulin release from the isolated perfused rat pancreas. | in the isolated perfused rat pancreas various sulfonylurea drugs were tested with a basal glucose level of 1 mg/ml in the perfusion buffer and were found to cause a biphasic insulin response. novocs 476, a new and potent sulfonylurea, and glibenclamide qualitatively differed from tolbutamide, glibornuride, glipizide, and glisoxepide, which were all alike in terms of the relationship between first and second phases of insulin release. | 1976 | 134920 |
| [bdellovibrio bacteriovorus as a factor in the self-purification of river water]. | 1976 | 134929 | |
| dysplasia of the tricuspid valve in the dog and cat. | dysplasia of the tricuspid valve in 14 dogs and 13 cats was studied. the clinical, electrocardiographic, radiographic, hemodynamic, angiocardiographic, and pathologic findings were reviewed in each species. alterations of the tricuspid valve complex included long, thick septal leaflets adhered to the septum; absent or short, stout fused chordae tendineae; hypertrophic fused papillary muscles; insertion of papillary muscles directly into the lateral leaflets; incomplete development of the valvula ... | 1976 | 134984 |
| [on the problem of atypical dysentery bacilli (author's transl)]. | 1976 | 135237 | |
| transplantation of hymenolepis diminuta into naive, immune and irradiated mice. | almost 100% of 7- to 10-day-old hymenolepis diminuta became established when surgically transplanted from donor mice into the duodenum of naive recipient mice. transplanted tapeworms survived 8-12 days, by which time they had survived much longer in total than they would have done in the donor. mice previously immunized by a primary infection rejected transplants within 4 days. sub-lethal irradiation (550 rad) delayed rejection by immune mice but such mice still rejected worms much more quickly ... | 1976 | 135242 |
| liver plasma membrane enzyme activities following glutaraldehyde fixation. | the wachstein-meisel atpase histochemical method has been previously used to demonstrate the ultrastructural localization of this enzyme in both whole liver and isolated plasma membranes following fixation in glutaraldehyde. in the present study biochemical assay, of liver plasma membrane enzymes following fixation in cold 2.5% glutaraldehyde showed that approximately 40% of mg2+-atpase, but only 4% of (na+-k+)-atpase activity remained in membranes from either control or anit-treated rats. in ad ... | 1976 | 135243 |
| cerium nitrate: a new topical antiseptic for extensive burns. | the wounds of 60 burned patients were treated topically with cerium nitrate, which was applied either as a cream or in aqueous solution. cerium nitrate has a potent antiseptic effect in human burn wounds, especially against gram negative bacteria and fungi. pseudomonas aeruginosa was recovered from the wounds infrequently and never predominated. fungi were practically never found. no patient treated with cerium developed a necrotizing wound infection. analysis of the detailed bacteriological dat ... | 1976 | 135364 |
| suppressor mechanisms in neonatally acquired tolerance to a gross virus-induced lymphoma in rats. | tolerance to a highly immunogenic gross virus-induced tumour in wistar/furth rats (c58nt)d was produced by neonatal infection of the rats with the virus. these rats failed to reject the tumours when challenged 8 weeks after virus inoculation and to mount the appropriate cell-mediated immune response to the tumour. the mechanisms involved were studied in vivo by adoptive transfers into sub-lethally irradiated rats of tumour cells mixed with spleen cells and/or sera from normal, tolerant, or tumou ... | 1976 | 135385 |
| effect of testosterone administration on the epiphyseal cartilage of hypophysectomized rats. | for the study of the mechanism of action of testosterone histological, carbohydrate-, and enzyme histochemical investigations were carried out on the epiphyseal cartilage of (1) hypophysectomized rats treated with testosterone [(thx group); (2)] hypophysectomized rats without hormonal treatment (hx group), and (3) intact, untreated control rats. the results were compared with the data obtained in a previous experiment in which intact rats were treated with testosterone (t group). the experiments ... | 1976 | 135467 |
| effect of vibrio cholerae neuraminidase on the generation of cell-mediated cytotoxicity in vitro. | vibrio cholerae neuraminidase (vcn))12.5 units/2 x 10(6) cells/ml) continuously present for a standard 5-day mlc will significant (p less than 0.02) increase the cytotoxic activity generated by a given number of responding spleen cells without reducing the specificity. heat-inactiviated vcn produced no such augmentation. this augmented cytotoxicity could be reproduced by preincubating (1 hr) the responding spleen cells with vcn (25 units/2.5 x 10(6) cells/ml) before addition of stimulating splee ... | 1976 | 136474 |
| tobacco, auxiliary etiologic factor of cancer of the larynx and hypopharynx]. | 1976 | 136491 | |
| induction of an autologous immune-complex glomerulonephritis in the rat by intravenous injection of heterologous anti-rat kidney tubular antibody iv: effect of injection of hgcl2 prior to the antibody. | autologous immune-complex glomerulonephritis developed in rats injected s.c. with hgcl2 2 days before the injection of anti-tubular fraction 3 antibody. the glomerulonephritis was progressive and characterized by granular deposition of igg and c-3, with proteinuria from the eighth week onwards. granular densities and severe glomerular basement membrane changes were observed when the experiment was terminated after 9 months. a possible mechanism of the glomerular lesion is discussed. | 1976 | 136979 |
| [reversal of the depressive power of cyclophosphamide on the anti-infectious defense of the mouse by means of a somatic antigen from bacillus subtilis]. | in the experimental conditions reported the cyclophosphamide increases the pathogenic effect of escherichia coli in mice. treating the animals with a somatic antigen of bacillus subtilis, reverses the aggravating effect of cyclophosphamide on the experimental infection. similar results are obtained through the parenteral and the rectal routes. this antigen does not limit either the leucopenient effect of cyclophosphamide nor its blocking effect on the synthesis of sheep red blood cells antibody ... | 1976 | 137039 |
| hyperkeratosis of the palms and soles associated with internal malignancy and elevated levels of immunoreactive human growth hormone. | 1976 | 137091 | |
| studies of the histochemistry, ultrastructure, motor innervation, and regeneration of mammalian intrafusal muscle fibres. | 1976 | 137430 | |
| [newly detected relationships between antibacterial activity and structure of some chalocones]. | 1976 | 130785 | |
| crosslinking of membranes: the effect of dimethylsuberimidate, a bifunctional alkylating agent, on mitochondrial electron transport and atpase. | 1976 | 130833 | |
| cleavage of thrombosthenin a by thrombin. evidence for the existence of two types of bovine platelet actin. | bovin platelet actin prepared by spudich's method (spudich, j. a. (1972) cold spring harbor symp. quant. biol. 27, 585-594) separated into two peaks on a sephadex g-200 column. the actin of both peaks had a mol. wt. of 42 000 on sodium dodecyl sulfate-polyacrylamide gel and activated myosin atpase, although in a quantitatively different manner. actin eluted in the first peak (probably an oligomeric form) was not polymerized in 2 mm mgcl2 and 0.05 m kcl, while that of the second peak went through ... | 1976 | 130929 |
| [spontaneous peripheral proteolysis/1st communication: method and results of clinical examinations (author's transl)]. | with a modificated astrup-fibrin plate-method also an inhibition of proteolysis can be registrated. in various medical areas a spontaneous peripheral proteolysis had been found, especially so in chronical bacterial infections. | 1976 | 132940 |
| [study of the incorporation of radioactive zinc in saccharomyces cerevisiae aldolase]. | saccharomyces cerevisiae aldolase concentrates from the culture medium containing znso4 a large amount of zn which becomes a component part of the enzyme molecular structure. this was made evident by adding to the culture medium 65znso4 and measuring the radioactivity of the aldolase extracted by a phillips single channel analyzer. | 1976 | 133445 |
| evidence of a (na+, k+) atpase activity in human circulating lymphocytes. | 1976 | 134750 | |
| [afferent projections to the locus coeruleus nucleus in the cat. study by the horseradish peroxidase technic]. | using a retrograde tracer technique with horseradish peroxidase, we have revealed some afferent projections to the locus coeruleus complex from the contralateral pontine tegmentum, raphe nuclei, substantia nigra, nucleus of the solitory tract, dorsal motor nucleus of the vagus and other regions of the ponto-bulbar reticular formation as well as from hypothalamic and preoptic areas. | 1976 | 134760 |
| [turnover of free adenine nucleotides of the isolated and perfused rabbit heart]. | the turnover of free adenine nucleotides was studied in the perfused rabbit heart by the decline in radioactivity 3 h 30 after administration of a single pulse of tritiated adenine. the radioactivity of the perfusate was followed during the experiments. about 7% of the labelled adenine nucleotides were renewed by hour and 1,5% of the radioactivity was lossed by hour in the perfusion fluid. | 1976 | 134762 |
| [motricity of the female genital apparatus in mammals. hormonal correlations]. | the motility and the pharmacological reactivity of the uterine tract of mammals are linked with the ovarian cycle and the gestation. in the birds, the study of general scheme of the hormonal mechanism and the uterine motility involued in the ovulation, the egg formation and the uterine dilatation can be used as an experimental model of the mammalian gestation. | 1976 | 134763 |