Publications

role of cyclic am in steroidogenesis in leydig cells: discrepancies' between effects of luteinizing hormone and cholera toxin.		1977	190030
intestinal secretion.		1977	190182
effects of cholera exotoxin on fc receptor activity of lymphoid cells and mononuclear phagocytes.	the effect of cholera exotoxin and aminophylline on fc receptors in a murine lymphoid-cell line and in rabbit pulmonary alveolar macrophages has been investigated. although both agents elevated intracellular cyclic amp levels in macrophages and lymphoid cells, the effects on fc receptor expression were distinct. cholera toxin at 10 microng/ml reversibly inhibited fc-receptor activity in the murine lymphoid cell line. in contrast, cholera toxin at 10 microng/ml or 0-01 microng/ml was ineffective ...	1977	191391
the number of receptors for beta-melanocyte stimulating hormone in cloudman melanoma cells is increased by dibutyryl adenosine 3':5'-cyclic monophosphate or cholera toxin.	cultured cloudman melanoma cells exposed either to dibutyryl 3':5'-cyclic amp and theophylline or to cholera toxin bind significantly more 125i-labeled beta-melanocyte stimulating hormone (msh) and fluorescein-labeled msh than untreated cells. msh binds to melanoma cells in the g2 phase of the cell cycle. the stimulation of msh binding by dibutyryl cyclic amp results from an increase in the number of msh receptors per g2 cell and, to a lesser extent, from an increase in the number of g2 cells. t ...	1977	191818
cholera toxin requires oxidized nicotinamide-adenine dinucleotide to activate adenylate cyclase in purified rat liver plasma membranes.	activation of adenylate cyclase in isolated rat liver plasma membranes by cholera toxin was demonstrated. the activation requires the presence of nad+ and atp and is irreversible.	1977	192202
synthesis and uptake of gangliosides by choleragen-responsive human fibroblasts.	human fibroblasts, cultured in medium containing 10% fetal calf serum, responded dramatically to choleragen with an increase in cyclic adenosine monophosphate content to greater than 48 times basal levels. analysis of these cells for gangliosides indicated that the major ganglioside was n-acetylneuraminylgalactosylglucosylceramide (gm3) with trace amounts (less than or equal to 100 pmol/mg of protein) of other gangliosides including gm1, the putative choleragen receptor. although the cells conta ...	1977	192274
enzymatic and chemical oxidation of gangliosides in cultured cells: effects of choleragen.	cell surface glycolipids of normal human fibroblasts and nctc2071 cells (transformed mouse fibroblasts) were labeled by incubating the intact cells with either galactose oxidase or sodium periodate, followed by reduction of the oxidized sugar residues with nab3h4. in intact human fibroblasts, incorporation of 3h was increased with increasing time of exposure to galactose oxidase prior to treatment with nab3h4. following limited exposure to galactose oxidase, more label was incorporated into the ...	1977	192275
arylamidases of rat liver and chemically induced hepatomas. ii. preparation and characterization of monospecific antisera against two distinct arylamidase-active antigens.	monospecific antisera were prepared against the most prominent arylamidase (alpha-aminoacyl-peptide hydrolase (microsomal), ec 3.4.11.2) active antigen in plasma membranes (the plasma membrane arylamidase) and lysomal content (the lysosomal content arylamidase), respectively. plasma membrane extract and lysosomal content were allowed to react in crossed immunoelectrophoresis against their homologous antisera. the electrophoretic plates were washed extensively, dried and subsequently stained for ...	1977	192299
the origin of acth cells in rat anterior pituitary.		1977	211898
effects of yunnan bai yao on blood platelets: an ultrastructural study.	the effects of yunnan bai yao on blood platelets were studied at an ultrastructural level. this herbal drug is found to cause the release of platelet constituents which accounts for its hemostatic effects. the fine structural changes of platelets in response to this drug are also described.	1977	204448
the influence of crude cottonseed oil in the feed on the blood and egg yolk lipoproteins of laying hens.	lipovitellin, very low density lipoproteins (vldl), low density lipoproteins (ldl), high density lipoproteins (hdl), and proteins of d greater than 1.20 were isolated from blood plasma and egg yolks obtained from hens fed a normal diet or one containing 2.5% of crude cottonseed oil. the amounts and compositions of each fraction were determined. hen blood plasma and egg yolk vldl and ldl obtained from hens fed a normal diet contained similar levels of lipid, and the fatty acid compositions of tho ...	1977	203909
closure of the human umbilical artery: a physiological demonstration of burton's theory.	30 umbilical cords of normal full-term infants were examined immediately after birth for closure of the arteries. multiple constrictions were observed in the arteries at birth, which increased in number and degree with time. between the constrictions there were segments of dilatations with trapped blood. cutting through the constrictions in the cords clamped later than 1 min after birth did not result in leakage of blood from the adjoining dilated segments which contained trapped uncoagulated bl ...	1977	264063
epidemiological analysis of factors influencing rate of progress in echinococcus granulosus control in new zealand.	the factors influencing the rate of progress in echinococcus granulosus control in new zealand were analysed by hydatid control area using stepwise multiple regression techniques. the results indicated that the rate of progress was related positively to initial e. granulosus prevalence in dogs and the efficiency with which local authorities implemented national control policy, and negatively to the maori proportion in the local population and the number of dogs per owner. problems in analysis of ...	1977	265340
isopeptide linkage between nonhistone and histone 2a polypeptides of chromosomal conjugate-protein a24.	chromosomal protein a24 has a unique structure inasmuch as it contains histone 2a and a nonhistone polypeptide the sequence of which has been partially determined. comparative analysis of the ninhydrin-insensitive amino-terminal tryptic peptides of protein a24 and histone 2a and a quantitative analysis of their carboxyl-terminal amino acid indicated that protein a24 has two amino termini and one carboxyl terminus. the amino acid sequence analysis of tryptic peptide 17' of protein a24: (see text) ...	1977	265581
chromosome banding patterns of 49 cases of chronic myelocytic leukemia.		1977	266114
thermotaxis by pseudoplasmodia of dictyostelium discoideum.	the temperature dependence of migration rate and of the thermotactic sensitivity of pseudoplasmodia of dictyostelium discoideum has been measured. migration rate increases with temperature to 20 degrees, is temperature insensitive from 20 degrees to 27.5 degrees, and decreases with temperature to 29 degrees, above which point migration ceases. however, pseudoplasmodia formed from cells grown at 23.5 degrees are thermotactic only from 22 degrees to 27.5 degrees. thus, a temperature dependence of ...	1977	266720
the representation of motor-unit action-potentials on skin-surface electromyograms of the masseter and temporal muscles in man.		1977	266875
unwinding protein specific for mrna translation fractionated together with rabbit reticulocyte initiation factor 3 complex.	experiments with a rabbit reticulocyte cell-free system dependent on the addition of initiation factor 3 (eif-3) and mrna were carried out. in this system, using ribosomal subunits, aug(u)(n) can direct polyphenylalanine synthesis in the absence of eif-3 at 3 mm mgcl(2). globin mrna was not translated under similar conditions; its translation requires the addition of eif-3. moreover, the maximal rate of globin synthesis was achieved when the molar ratio of eif-3 to ribosomes was approximately 1. ...	1977	267926
role of perivascular sympathetic nerves in autoregulation of cerebral blood flow and in blood-brain barrier functions.		1977	268889
an autoradiographic study of the sensory innervation of teeth. ii. dental pulp and periodontium.	autoradiography of axoplasmically transported proteins revealed that both dental pulp and periodontium receive sensory innervation from the trigeminal ganglion in the form of plexuses and free and organized sensory receptors, including apparent corpuscular ones. corpuscular receptors in the pulp suggest this tissue may be responsive to modalities other than pain.	1977	269163
ultrastructural localization of cell membrane gm1 ganglioside by cholera toxin.	an immunoelectron microscopic method is described for sensitive high-resolution visualization of tissuebound cholera toxin. the principle is to incubate cells or tissue sections with toxin and then to localize the bound toxin with toxin-specific peroxidase (donor:hydrogen-peroxide oxidoreductase; ec 1.11.1.7)-conjugated antibody and enzyme substrate. thin sections are examined for electron-opaque precipitates in a transmission electron microscope. because of the specific binding of the toxin to ...	1977	269432
loss of growth control and differentiation in the fu-1 variant of the l8 line of rat myoblasts.	a nonfusing variant, fu-1, of the l8 line of rat myoblasts was isolated and characterized with respect to its growth in vitro and developmental properties. comparative analyses of density-dependent inhibition of growth, serum requirements, cell adhesiveness, colony formation in soft agar, and hexose transport in l8 and fu-1 cells support the conclusion that the fu-1 cells are transformed. in addition, fu-1, but not l8, cells promote the development of tumors in athymic nude mice. fu-1 cells also ...	1977	269441
comparative cariogenicity of streptococcus mutans strains isolated from caries active and caries resistant adults.		1977	270487
in vitro enamel demineralization by streptococcus mutans in the presence of salivary pellicles.	salivary pellicles developed on extracted teeth favorably affected the degree and nature of enamel demineralization when the teeth were incubated in vitro with either of two pure strains of cariogenic streptococcus mutans. the mechanism responsible for this protection may relate to the permselective properties of these salivary pellicles.	1977	270509
effect of galactose oxidase, with and without prior sialidase treatment, on the viability of erythrocytes in circulation.	previous studies have shown that sialidase-treated mammalian erythrocytes were rapidly eliminated from circulation. in contrast, chicken asialoerythrocytes remained fully viable. this investigation was undertaken to ascertain the reason for this difference in behavior as well as to determine the extent of the similarity of the physiological mechanism for the elimination from circulation of asialoglycoproteins and mammalian asialoerythrocytes. to that end, erythrocytes from dogs, rabbits, and chi ...	1977	270664
participation of providence senior citizens in the swine flu inoculation program.		1977	270786
binding of atp to progesterone receptors: properties and functional significance of this interaction.		1977	281180
absorption tests on cold lymphocytotoxins: preliminary report.	tests were carried out to absorb cold lymphocytotoxic antibodies on different types of cells. these included lymphocytes and neuraminidase-treated lymphocytes from healthy donors, thymocytes from children undergoing open heart surgery, fetal thymocytes, human brain and cultured lymphocytes. the results showed these antibodies to be absorbed on some types of cells and eluted from others. the best absorption was obtained with fetal thymocytes and brain tissue.	1977	302062
analysis of lymphocyte surface antigen expression by the use of variant cell lines.		1977	302193
sensitivity of the vibrios to ultraviolet-radiation.	the ultraviolet-inactivation kinetics of a number of strains of vibrio cholerae (classical), vibrio cholerae (el tor), nag vibrios and vibrio parahaemolyticus were investigated. statistical analyses revealed significant differences between any two of the four types of vibrio in respect to their sensitivity to u.v.	1977	302245
immunosuppression in man: suppression by macrophages can be mediated by interactions with regulatory t cells.		1977	302274
[incidence of infant tuberculosis in germany during the first year after cessation of unselected bcg vaccination (author's transl)].	at the end of may 1975, bcg vaccine was withdrawn from the market in the federal republic of germany, because a newly introduced vaccine had given rise to untoward reactions. neonatal vaccination practically ceased. the results of a survey by questionnaire, undertaken on behalf of the vaccination committee of the german society for social paediatrics, are reported. from 1.9.1975 to 31.8.1976 the incidence of tuberculosis in the first year of life had already doubled compared with the reported an ...	1977	302786
cell-mediated immunity to tumor antigen in marek's disease: susceptibility of effector cells to antithymocyte serum and enhancement of cytotoxic activity by vibrio cholerae neuraminidase.	spleen cells from chickens inoculated 7 to 8 days previously with marek's disease virus were cytotoxic for 51cr-labeled cells of a marek's disease lymphoblastoid cell line (msb-1 line) in a 4-h in vitro cytotoxic assay. the cytotoxic activity of spleen cells was inhibited by pretreatment with antithymocyte serum and complement, but not with complement alone or in combination with anti-bursa cell serum or normal preimmune serum. the conclusion was that the effector cell in the above cytotoxic ass ...	1977	302827
human b lymphocytes in giemsa stained preparations.	a mixed antiglobulin procedure has been developed to provide simultaneous study of cell surface immunoglobulin and detailed cytological features in giemsa stained preparations. the percentage of surface immunoglobulin bearing lymphocytes, monocytes and granulocytes changed dramatically when lymphocytes were incubated at 37 degrees c, or following sheep erythrocyte rosette enrichment procedures.	1977	302844
natural cell-mediated immunity to lymphoma cells. i. characteristics of effector cells in a cytostasis assay in vitro.	spleen cells from normal, nonimmune, cba or (cba x akr)f1 mice markedly and rapidly inhibited the incorporation of [3h]thymidine by two different t-cell lymphomas in an in vitro cytostasis assay. these were the i-529 lymphoma of spontaneous akr origin and the moloney murine leukemia virus-induced yac lymphoma of a mouse origin. spleen cells were the most efficient inhibitors for both types of target cells, whereas lymph node cells were much less active and thymus cells showed little or no activi ...	1977	302867
[cellulitis and bacteremia caused by haemophilus influenza group b].		1977	302886
freezing of rat lymphocytes. iv. in vitro and in vivo responses of t-cells.	rat lymph node lymphocytes have been frozen-thawed with 10% dimethyl sulphoxide as cryoprotectant. the present study shows that these cells respond well in t-cell functional assays, such as stimulation with phytohaemagglutinin, as responder cells in the mixed lymphocyte reaction and in the local graft-versus-host assay. the magnitude of the responses obtained with frozen-thawed cells was some 70-80% of corresponding fresh cells.	1977	303436
ontogeny of myelopoietic precursor cells in the chicken embryo.	the agar culture technique which enables enumeration of myeloid precursor cells was used to establish the time of appearance and further development of these cells in chick embryos. yolk mesenchyme is the largest producer of precursor cells from day 6 to day 17 of incubation. in circulating blood precursor cells probably from the yolk appear at day 9 and stay at fairly high levels till day 17. spleen and bone marrow have high percentage of precursor cells from day 10 till hatching, but afterward ...	1977	303449
induction in b2/b2 chickens of immunity to transplantable carcinogen-induced fibrosarcomas mediated by t-cell monocyte cooperation: role of delayed hypersensitivity to unrelated antigens.		1977	303451
neuraminidase- and trypsin-induced exposure to membrane receptors for igg and igm molecules on human peripheral blood lymphocytes.	brief incubation of human peripheral blood lymphocytes with vibrio cholerae neuraminidase (vcn) or trypsin revealed hidden membrane receptors for igg and igm molecules. the hidden receptors were found on t lymphocytes as shown by double-label and mixed rosetting experiments and by studies of t-enriched populations. although igm receptors were undetectable on freshly isolated lymphocytes, a mean of 17.1% of vcn-treated lymphocytes rosetted with ox erythrocytes coated with igm antibody (ea-igm). p ...	1977	303965
[histochemistry of lipids and carbohydrate in the rat skin. the sebaceous gland and its duct].		1977	305621
[pharmacologic properties of ortho-ethoxy-benzamide].		1977	305744
x-linked recessively inherited non-specific mental retardation. report of a large family.	a large kindred is described in which 22 males and 3 females show non-specific mental retardation with impaired speech. an x-linked recessive is the most likely mode of inheritance of this condition. similar families have been described in the literature, characteristic physical abnormalities are absent and performance i.q. tends to be higher than verbal i.q. this possible heterogenous condition may be a major individual cause of mental deficiency in males, and may account for the excess of male ...	1977	305754
partial duplication 5q syndrome: phenotypic similarity in two sisters with identical karyotype (partial duplication 5q33 leads to 5qter and partial deficiency 8p23 leads to pter).	two sisters with statomotor developmental retardation microcephaly, hydrocephalus internus and externus without signs of pressure, heart defect (ventricular septal defect), early pulmonary resistance and characteristic facial changes were found to have the same unbalanced karyotype with partial trisomy 5q3300 leads to 5qter and partial monosomy 8p2300 leads to 8pter, derived from a balanced reciprocal paternal translocation: 46,xy,t(5;8)(q3300;p2300). the older girl was tested for the erythrocyt ...	1977	305758
prevention of implantation by [d-trp6-lh-rh in the rat: comparative study with the effects of large doses of hcg on pregnancy.	effect of large doses of [d-trp6]-lh-rh and hcg on implantation of fertilized ova was examined in the rat. subcutaneous administration of 6ug[d-trp6]-lh-rh per day from days 1 to 5 of pregnancy by an implanted minipump completely prevented the implantation of fertilized ova associated with a dramatic reduction of plasma progesterone on days 4 and 8, with a slight reduction of estradiol on day 4. there was no balooning of the uterus in the exploratory laparatomy on days 8 and 14. administration o ...	1977	350578
pattern of haemagglutination and haemolysis tests in v. cholerae biotype el tor recently isolated from howarah hospital.		1977	350772
antibodies in human serum and milk induced by enterobacteria and food proteins.	ingestion of escherichia coli o83 bacteria by adults resulted in a transient irregular colonization leading to a serum antibody response in only four out of 14 cases examined. in all of three pregnant women, however, iga antibodies against e. coli o83 antigen were released from colostral cells after similar bacterial ingestion although no serum antibody response was noted. the findings indicate a link between the antigenic exposure to the gut and secretory antibodies of the iga class, presumably ...	1977	346319
studies on immunologic cross-reactions of enterotoxins from escherichia coli and vibrio cholerae.		1977	342403
immunological properties of vibrio cholerae lipopolysaccharides.	immunological effects of wall lipopolysaccharide (lps) preparations obtained from vibrio cholerae inaba 569b, ogawa nih 41 and nag 4715 strains by the hot phenol-water procedure were examined in mice. although these lps lack kdo, which are basic components of the core region of most gram-negative lps, they still have potencies as b-cell mitogens, adjuvants, immunosuppressants, polyclonal b-cell activators and phagocytic stimulants for macrophages. the activities of these v. cholerae lps on murin ...	1977	342874
acute gastro-enteritis (a clinical and bacteriological study).		1977	344805
immune function in patients with multiple myeloma (delayed cutaneous reactivity and lymphocytes bearing receptors for sheep, human and mouse erythrocytes).	immunologic function was evaluated in 27 patients with multiple myleoma (mm). when tested for their ability to develop delayed hypersensitivity to panel of skin test antigens, 2 out of 15 such patients were found to be anergic. the property of human peripheral blood t lymphocytes to form rosettes with sheep [total e and active or early rosette-formimg cells (rfc)] and human erythrocytes (h-rfc) was also studied. in addition, rosette formation with mouse erythrocytes (m-rfc) was investigated as a ...	1977	307801
the lipid content of epididymal spermatozoa of rattus norvegicus.	1. seven major lipids of rat spermatozoa from the caput, corpus and cauda epididymidis were separated and quantitated by tlc. 2. spermatozoa from the caput epididymidis had a significantly greater (p less than 0.05) content of phospholipid, cholesterol, cholesterol ester and free fatty acid than those from the cauda epididymidis. 3. spermatozoa from the corpus epididymidis had a significantly greater (p less than 0.05) content of monoglyceride than those from the caput epididymidis and a greater ...	1977	318275
[immunoblastic sarcoma (reticulum-cell sarcoma) of the gastro-intestinal tract in a case of dermatitis herpetiformis duhring (author's transl)].	in a 43-year-old man who for 20 years was known to have dermatitis herpetiformis there co-existed, since 1974, a "dermatogenic enteropathy" with gastro-intestional symptoms. several small-intestine biopsies demonstrated severe partial to subtotal villous atrophy with crypt hyperplasia, as seen in gluten-sensitive enteropathy. at post-mortem there was an immunoblastic sarcoma - so-called reticulum-cell sarcoma - in the upper jejunum and abdominal lymph-nodes, which was presumably the immediate co ...	1977	318987
induction of escherichia coli and vibrio cholerae enterotoxins by an inhibitor of protein synthesis.	enterotoxigenic escherichia coli or vibrio cholerae 569b (inaba) grown in the presence of the antibiotic lincomycin, an inhibitor of protein synthesis, produced elevated levels of heat-labile enterotoxin or choleragen, respectively, as assayed by both vascular permeability factor and capacity to elicit fluid accumulation in rabbit ileal loops. this induction of enterotoxin did not reflect either a coupling of lincomycin resistance with increased enterotoxigenicity or an effect of lincomycin on c ...	1977	319055
temperature-sensitive r plasmid obtained from naturally isolated drug-resistant vibrio cholerae (biotype el tor).	a temperature-sensitive fi(-) r plasmid, pjy1, which confers resistance to chloramphenicol, streptomycin, and sulfonamide, was found in a drug-resistant strain of vibrio cholerae (biotype el tor) isolated in the philippines in 1973. the r plasmid is temperature sensitive for transferability but not for stability in the hosts. pjy1 is stably maintained either in v. cholerae or in escherichia coli at a range of culture temperatures from 27 to 42 degrees c. compatibility tests with reference r plas ...	1977	319746
[bovine renal tuberculosis in a farmer--contribution to the relationship between human and bovine tuberculosis].		1977	319986
comparison of the virulence of o:9,12 and o:4,5,12 salmonella typhimurium his+ transductants for mice.	the purpose of these experiments was to determine whether qualitative differences in the o repeat unit of the lipopolysaccharide of pathogenic salmonella species affected virulence for mice. o:4,5,12 and o:9,12 sister his+ transductants were derived from a virulent salmonella typhimurium. fermentation markers were introduced by transduction to differentiate these strains, and the strains were used as mixed challenge to cf1 and c57b1/6j mice. liver and spleen cultures of the mice of various inter ...	1977	321351
use of fluorescent antibody in studies of immunity to cholera in infant mice.	infant mice 8 days of age were infected orally with virulent, motile, classical or el tor strains of vibrio cholerae and with nonmotile mutants of low virulence derived from the same strains. at intervals of 8 and 12 h postinfection, frozen thin sections of the ileum were prepared, stained with fluorescein isothiocyanate-labeled rabbit anti-vibrio antibody, and examined with the fluorescence microscope. the motile organisms were present in larger numbers, especially at 12h, and had penetrated th ...	1977	321353
new culture medium for the presumptive identificaion of candida albicans and cryptococcus neoformans.	a new medium composed of tween 80, oxgall, caffeic acid, and davis agar (toc) that provides for the rapid presumptive identification of candida albicans and cryptococcus neoformans is described herein. c. albicans is differentiated from other yeasts by the sequential production of germ tubes and chlamydospores. in a comparison with cormeal agar control plates, there was an increase of chlamydospore-forming strains of c. albicans (97.1% versus 87.2%) and a decrease in the time required for chlamy ...	1977	321472
diarrhea due to escherichia coli in the rabbit: a novel mechanism.	a strain of escherichia coli o15 (rdec-1) isolated from several rabbits with diarrhea was examined to determine (1) whether the strain could produce diarrhea when administered by the orogastric route to other rabbits and (2) whether this strain was invasive or enterotoxigenic. strain rdec-1 produced diarrhea in 48 of 62 rabbits when given by the orogastric route in doses that ranged from 1.5 x 10(2) to 4 x 10(10) bacteria. the organism did not give a positive result in the 18-hr ileal loop or se ...	1977	321703
characterization of the chinese hamster ovary cell assay for the enterotoxins of vibrio cholerae and escherichia coli and for specific antisera, and toxoid.	the morphologic change in chinese hamster ovary (cho) cells that is associated with increased intracellular concentrations of adenosine 3':5'-cyclic phosphate (cyclic amp) provided a highly sensitive assay for the heat-liable enterotoxins of vibrio cholerae and escherichia coli. the similarity between the responses of cho cells and those of intestinal mucosa to enterotoxins was demonstrated by the inhibition of these effects by the ganglioside galactosyl-n-acetylgalactosaminyl [sialosyl] lactosy ...	1977	323378
the role of chemotaxis in the ecology of bacterial pathogens of mucosal surfaces.		1977	323724
[history of microbiology in japan (6)].		1977	325250
reduction of reactivity of escherichia coli enterotoxins by intestinal mucosal components.	incubation studies involving rabbit and piglet small intestinal mucosal components and escherichia coli and vibrio cholerae enterotoxins were conducted at 37 and 4 degrees c. mucosal homogenate cytosol from rabbits did not significantly alter the reactivities of either cholera enterotoxin (ct) or e. coli labile enterotoxin (lt). however, mucosal homogenate cytosol from piglets was capable of neutralizing lt, though it had no effect on e. coli stable enterotoxin. lt became bound to piglet and rab ...	1977	326677
solubilization of chromatin with heparin and the isolation of nuclear membranes.		1977	327198
cholera old and new.		1977	327479
a bactericidal effect for human lactoferrin.	streptococcus mutans and vibrio cholerae, but not escherichia coli, were killed by incubation with purified human apolactoferrin. concentrations of lactoferrin below that necessary for total inhibition resulted in a marked reduction in viable colony-forming units. this bactericidal effect was contingent upon the metal-chelating properties of the lactoferrin molecule.	1977	327545
recent developments in diarrheal diseases.	diarrheal diseases result from two different processes: toxin elaboration by pathogens such as vibrio cholerae and some strains of escherichia coli and invasion of tissue, eg, by shigellae and salmonellae. intestinal motility serves as a normal cleansing mechanism of the intestine, and drugs that decrease this motility may facilitate replication of pathogens and their attachment to or invasion of the intestinal tissue. therapy should not be aimed at suppressing the symptom of diarrhea. it is now ...	1977	329247
[role of antibiotics in the ecology of cholera vibrios].		1977	329747
[disinfecting effect of fixatives in histoenzymatic analysis of vibrio cholerae].	data are presented on the disinfecting action of fixative fluids on the cholera causative agents in the freshly frozen sections from the tissues and organs of animals infected with cholera. the results obtained offered a possibility of drawing a conclusion on the harmlessness of the histoenzymatic studies on the material infected with cholera vibrio.	1977	331762
[trial of the toxins of the sonne microbe and cholera vibrio in an isolated loop of mouse small intestine].	enterotoxic properties of neuro-and endotoxins of sonne dysentery bacillus, of cholerogen, and bowen's cholera vibrio endotoxin were determined on a model of an isolated loop of the mouse small intestine. in definite doses all the mentioned preparations could induce dilatation of the isolated loop of the mouse small intestine. in case of sonne bacillus neurotoxin this property correlated with the toxicity of the preparation for the animals and failed to depend on the virulence and biochemical re ...	1977	331786
the problems of oral immunisation.		1977	332136
identification and treatment with a long-acting sulphonamide of 15 carriers of vibrio cholerae el tor serotype ogawa, during the 1973 cholera epidemic in naples.	during the cholera epidemic in 1973, nine contact carriers of vibrio cholerae el tor serotype ogawa were identified in a family from naples as well as six others coming from different parts of the city or its surroundings. all the subjects were admitted to the quarantine ward of the 'cotugno hospital' (naples) in which they remained continuously for 14 or 16 days. during this time these bacteriologically confirmed carriers were treated orally with a long-acting sulphonamide (sulphamethoxypyrazin ...	1977	332466
microtiter solid-phase radioimmunoassay for detection of escherichia coli heat-labile enterotoxin.	the development of a microtiter solid-phase radioimmunoassay for the detection of vibrio cholerae enterotoxin and heat-labile escherichia coli enterotoxin is described. the test is based on the immunological similarity between v. cholerae toxin and e. coli heat-labile toxin. the assay is easy to perform, quantitative, and at least as sensitive and specific as the y-1 adrenal cell system.	1977	332626
algorithms in the diagnosis and management of exotic diseases. xxvi. cholera.		1977	333039
bismuth subsalicylate inhibits activity of crude toxins of escherichia coli and vibrio cholerae.	the inhibition of the activity of enterotoxins by bismuth salts was assessed in laboratory models. in the y-1 adrenal cell tissue culture system, a commercial preparation containing bismuth subsalicylate reduced the activity of crude toxin from vibrio cholerae by 10(4)-fold as compared with the activity of controls. similar results were obtained with use of the adult rabbit ligated intestinal loop model. the preparation failed to affect crude escherichia coli or cholera toxin activity once these ...	1977	335003
systemic effect of vibrio cholerae and escherichia coli enterotoxins on plasma glucose and alkaline phosphatase.		1977	336525
enzyme-linked immunosorbent assay for detection of escherichia coli heat-labile enterotoxin.	the development of an enzyme-linked immunosorbent assay (elisa) for the detection of heat-labile escherichia coli enterotoxin is described. the assay, which is based on the immunological similarity between vibrio cholerae toxin and heat-labile e. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. the elisa system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat ...	1977	336638
cross-neutralization reactions of escherichia coli and vibrio cholerae enterotoxins as studied by rabbit skin inoculation test.	neutralization of enterotoxins of vibrio cholerae 569 b and escherichia coli 10407 by antitoxins to v. cholerae 569 b, e. coli 334, 408-3 and 10407 was studies by intradermal inoculation test in the rabbit. neutralization of v. cholerae enterotoxin by homologous as well as heterologous antisera of e. coli was observed, except that there was no neutralization of the enterotoxin by antiserum to e. coli 408-3 enterotoxin. neutralization of e. coli enterotoxin to a varied extent by homologous as wel ...	1977	337760
[new methods of laboratory diagnosis of plaque and cholera].		1977	339614
enterotoxin antibodies in relation to diarrhoea in swedish soldiers in cyprus.	the development of antibodies to escherichia coli heat-labile enterotoxin (lt) and its relationship to diarrhoea was studied in two swedish battalions (one in the winter, wb, and one in the summer, sb) of the united nations peace-keeping force in cyprus. whereas only 6-15% of the soldiers had detectable levels of anti-lt antibodies in their sera before leaving sweden, as many as 69% of the sb and 43% of the wb soldiers who developed diarrhoea in cyprus acquired significant titres of enterotoxin ...	1977	340076
the agglutination reactions of cholera vibrios.	the present study with 11 strains of vibrio using single-dose and hyperimmune antisera confirmed earlier observations on the cross-reactivity of the flagellar (h) agglutinating antigens of cholera and nag vibrios. the effect of several variables on the agglutinating sensitivity of cell suspensions was determined by measuring the reaction rate in the presence of constant o- or h-antibody. the variables investigated were culture conditions, antigen dilution, reaction temperature, formalin fixation ...	1977	340734
cholera: the relevance of the irrelevant.		1977	341599
cholera toxin, ganglioside receptors and the immune response.	cholera toxin activates plasma membrane adenylate cyclase in all mammalian cell types. the structure-function relationship of the toxin has recently been clarified, and the cell membrane receptor identified. this information has made cholera toxin the "agent of choice" for studies in many biological systems of the possible regulatory role of adenylate cyclase/cyclic amp. this article describes briefly our current knowledge about the toxin and its receptor. it then reviews recent research which h ...	1976	192668
differential effects of prostaglandin synthetase inhibitors on phosphodiesterase activity and choleragen-induced cycly amp production in rabbit uterus.		1976	190187
lytic action of polymyxin b on the liposomes prepared from vibrio cholerae phosphilipid.		1976	188755
[cyclic nucleotides and intestinal function].		1976	189126
[effect of 2,6-diaminopurine resistance mutations on adenine and adenosine assimilation by cells of an adenine-dependent strain of escherichia coli k-12].	in purine-requiring strain of escherichia coli k-12 defective in purine nucleoside phosphorylase (pur, pup) mutants (designated apt) have been obtained that are resistant to 2,6-diaminopurine on guanine-containing medium and incapable to utilize adenine for their growth at 42degreesc, but they are still sensitive to the analogue and can utilize adenine at 28degreesc. it has been shown that the introduction of the corresponding apt mutations in the genome of adenine-requiring strains impaired the ...	1976	187528
role of active sodium transport in brown fat thermogenesis.		1976	187559
spin-labeled stearates as probes for microenvironment of murine thymocyte adenylate cyclase-cyclic adenosine 3':5'-monophosphate system.	the interaction of various spin-labeled compounds with the murine thymocyte adenylate cyclase-cyclic amp system was investigated. electron paramagnetic resonance spectra from spin-labeled compounds were used to calculate the order parameter, s, and indicated that the thymocyte plasma membrane is a relatively rigid structure. increasing concentrations of spin-labeled stearates, but not their corresponding methyl esters, resulted in increased membrane fluidity, partial lysis, and concomitant compl ...	1976	187588
hydrolysis of nicotinamide adenine dinucleotide by choleragen and its a protomer: possible role in the activation of adenylate cyclase.	choleragen and the isolated a protomer catalyzed the hydrolysis of nad to adp-ribose and nicotinamide. the protein with nadase activity (nad nucleosidase; nad glycohydrolase, ec 3-2-2-5) migrated on polyacrylamide gels with choleragen, and chromatographed on bio-gel p-60 columns with the a protomer. the nadase activity of choleragen and of the a protomer was increased markedly in acetate and phosphate buffers, and enhanced over 10-fold by dithiothreitol in high concentration. nad hydrolysis was ...	1976	188038
effects of choleragen on hormonal responsiveness of adenylate cyclase in human fibroblasts and rat fat cells.	choleragen increases cyclic amp content of confluent human fibroblasts. maximally effective concentrations of isoproterenol and prostaglandin e1 also induce large increases in cyclic amp content of human fibroblasts and in confluent cultures the effect of prostaglandin e1 is much greater than that of isoproterenol. after incubation with choleragen, the increment in cyclic amp produced by 2 mum isoproterenol is increased and approaches that produced by5.6 mum prostaglandin e1. although the concen ...	1976	188460
histochemistry of oxidative enzymes in experimental allergic encephalomyelitis.	the histoenzymic pattern of oxidative enzymes (g3pd, idh, sd, g6pd,hbd, nadph: dehydrogenase) was investigated in experimental allergic encephalomyelitis (eae) produced in rats according to paterson [13]. the results obtained lead to following conclusions: (1) the neuroglia, including the white matter oligodendroglia of immunized rats, exhibits increased oxidoreductase activities; (2) the neuroallergic reaction induces some stimulation of the oxidoreductive metabolism of oligoden-droglia; (3) th ...	1976	186370
microtubule assembly in cultivated greene melanoma cells is stimulated by dibutyryl adenosine 3':5'-cyclic monophosphate or cholera toxin.	both dibutyryl cyclic amp (dbcamp) and cholera toxin promote the formation and elongation of processes of cultivated greene hamster melanoma cells. the formation and maintenance of these processes, which contain many microtubules, are sensitive to colcemid and vinblastine. tubulin was measured by [3h]colchicine binding and by acrylamide gel electrophoresis. we found that dbcamp or cholera toxin increases the ratio of polymerized to unpolymerized tubulin but not the total amount of tubulin per ce ...	1976	186462
plasma 16 beta-hydroxydehydroepiandrosterone in normal and pathological conditions in man.	plasma 16beta-hydroxydehydroepiandrosterone (16 beta-oh-dhea) levels in normal subjects and patients with certain pathological conditions have been evaluated using radioimmunoassay of the steroid. plasma 16 beta-oh-dhea levels in normal subjects rose sharply during adolescence and then declined slowly throughout adult life: 192 +/- 54 (se) pg/ml between 7 and 11 yrs., 395 +/- 22 pg/ml between 15 and 19 yrs, 330 +/- 29 pg/ml between 20 and 39 yrs., 291 +/- 35 pg/ml between 40 and 59 yrs., and 124 ...	1976	186475
the release of endogenous amino acids from the rat visual cortex.	the release of endogenous taurine, gaba, glycine, aspartate, glutamate, glutamine and alanine from the rat visual cortex was measured using a cortical cup technique. the electrocorticogram (ecog) was monitored throughout most experiments. 2. spreading depression, evoked by the dropwise placement of 10% kcl solution on to the brain outside the cup was associated with a significant increase in the release of gaba and glutamine but a marked fall in that of glutamate. the evoked release of gaba and ...	1976	186588
proteolytic enzyme activity in the granulation tissue of the human burn wound.	a procedure for adsorbing enzymes from the human burn wound onto solid sheets of substrate is described. using this technique, low levels of enzyme activity with chymotrypsin-like specificity can be demonstrated in the wound approximately 2 weeks after injury. this activity disappears at about 5 weeks after the burn. the enzyme activity corresponds with the clinical experience for the time course of natural loss of the burn eschar. a trypsin-like enzyme of very low level activity is present in t ...	1976	186629
effects of cholera exotoxin on the mitochondrial morphology of lymphoid cells and mononuclear phagocytes.		1976	186665
[behavior of certain parameters of lipid and energy metabolism. 3. relationships between cyclic 3',5'-adenosine monophosphate and acetyl coenzyme a in liver of growing rats deduced from model experiments with diets differeing in fat content].	schemes of the lipid and the pyruvate metabolism serve to show that a great part of the enzymes which intervence in the metabolic pathways and are associated with the formation and the consumption of acetyl coenzyme a may be regulated by cyclic 3',5'-adenosine monophosphate (camp) in the sense of activation or inhibition. the camp increase in the liver, which has been demonstrated in the present study for a diet containing 25% of fat, opens the metabolic pathway to the formation of acetyl coenzy ...	1976	186712
[infections transmitted in swimming pools].	public swimmingpools can be the source of infections due to micro-organism such as mycobacterium balnei, adeno and enteroviruses, the virus of plantar warts and molluscum contagiosum, the tric-agent of swimmingpool-conjonctivitis and pathogenic fungi. the transmission of trichomonas vaginalis is considered unlikely-water of pools, supposed to present satisfactory qualities by standard controls, was found to contain pathogenic staphylococci and pseudomonas aeruginosa. effective preventive measure ...	1976	186978