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studies on the antigenic relationship between an isolate (127) from the egg drop syndrome 1976 and a fowl adenovirus.birds infected with fowl adenovirus type 1 (fav-1) developed precipitating antibody to fav-1 but not to haemagglutinating virus 127 isolated from flocks with a depressed egg production syndrome. once the precipitating antibody to fav-1 was no longer detectable, the birds were then infected with 127 virus. they developed precipitating and haemagglutination-inhibiting antibody to 127 virus. they also developed precipitating antibody to the adenovirus group antigen. birds infected only with 127 vir ...197818770415
procedures for the haemagglutination and the haemagglutination inhibition tests for avian infectious bronchitis virus.various aspects of production, storage and stability of the haemagglutination (ha) activity of infectious bronchitis virus (ibv) were studied. from the results obtained, a standard procedure for the production of ibv, ha and the ha and haemagglutination inhibition (hi) tests is suggested. the main points of the suggested procedure are: (1) the virus should be concentrated but not purified; (2) the virus should be treated with phospho-lipase c type 1 (plc), at a final concentration of one unit/ml ...197718770308
influence of maternal antibodies on vaccination of chicks of different ages against infectious bronchitis.in chicks from immune hens levels of antibody to infectious bronchitis (ibv) measured by the neutralisation test (ni values) decreased linearly with age. in 1-day-old chicks ni values were high, whereas they were zero in 30-days-old birds. vaccination of 1-day-old chicks with high ni values by the conjunctival and intranasal routes with h 120 vaccine virus resulted 4 weeks later in an immunity that was as good as that obtained by vaccination of 20- and 15-days-old birds with lower levels of mate ...197718770311
[respiratory diseases of the fowl:principles of histopathological diagnosis].groups of specific pathogen free chickens were inoculated with five strains of newcastle disease virus, one strain of adenovirus, four strains of infectious bronchitis virus, one strain of mycoplasma gallisepticum and with infectious bronchitis virus together with mycoplasma gallisepticum. lungs and tracheas were taken at regular intervals for histopathological examination. a diagnosis could be based on tracheal lesions at different periods of their development. newcastle disease was distinguish ...197718770313
the agar-gel precipitin response to the h120 and h52 vaccines of infectious bronchitis virus.the respiratory and agar-gel precipitin response of chickens to h120 and h 52 vaccines of infectious bronchitis virus (ibv) were studied. on aerosol exposure of 3-weeks-old chicks to h120, slight respiratory symptoms were observed in 30% while 70% produced precipitins. eleven-weeks-old chicks inoculated intratracheally with h52 alone showed severe symptoms and 100% produced precipitins. similar birds previously vaccinated with h120 showed no symptoms on challenge with h52 but 55% produced precip ...197718770322
incidence of pasteurella haemolytica in the respiratory tract of apparently healthy chickens and chickens with infectious bronchitis. characterisation of 213 strains.the tracheal flora of healthy chickens consisted mainly of gram-positive microorganisms. a shift to a predominantly gram-negative flora, mainly due to escherichia, was observed in chickens with respiratory symptoms caused by infectious bronchitis. most of the chickens in which e. coli formed a predominant part of the tracheal flora also showed a positive serological reaction to mycoplasma gallisepticum. a raised incidence of pasteurella haemolytica with increase in age was noted both with regard ...197718770338
serological studies on flocks showing depressed egg production.a serological investigation was undertaken of flocks with depressed egg production. no obvious correlation was found between antibody to adenovirus, infectious bronchitis virus or infectious bursal disease virus and this syndrome. no antibody was detected to newcastle disease virus, mycoplasma gallisepticum or in most cases to mycoplasma synoviae. a relationship was found between the development of antibody to a haemagglutinating virus, 127, and depressed egg production. a retrospective survey o ...197718770349
studies on avian infectious bronchitis virus in new zealand. i. serotypes. 1977208028
studies on avian infectious bronchitis virus in new zealand. ii. properties of four serotypes at a low and a high passage level. 1977208029
studies on avian infectious bronchitis virus in new zealand. iii. efficacy of an attenuated live infectious bronchitis vaccine of new zealand origin in broiler birds. 1977208030
unexpected isolation of a newcastle disease virus. 1977207254
unexpected isolation of a newcastle disease virus. 1977207255
the purification and polypeptide composition of avian infectious bronchitis virus.purification of egg-grown infectious bronchitis virus (ibv) by sucrose density gradient centrifugation alone, or sucrose density gradient centrifugation plus ph 8.0 treatment, concanavalin a precipitation or metrizamide density gradient centrifugation, failed to produce any differences in the virus polypeptide pattern following polyacrylamide gel electrophoresis in the presence of sds(sds-page). sds-page of purified ibv on 7.5% acrylamide gels separated 16 polypeptides which were detectable by s ...1977205761
influence of environment on airsacculitis: effects of relative humidity and air temperature on broilers infected with mycoplasma synoviae and infectious bronchitis.mycoplasma synoviae (ms) obtained from broiler chickens condemned for airsacculitis was used to determine the influence of air temperature and relative humidity on the severity of airsacculitis produced experimentally. infectious bronchitis virus was administered to 3-week-old broilers 5 days before aerosol exposure to ms broth cultures, producing extensive airsacculitis within 21-day study periods. high (31-32 c), medium (19-24 c), and low (7-10 c) air temperatures were studied in conjection wi ...1977194567
comparative ultrastructural studies on endemic (balkan) nephropathy and chicken embryo nephritis caused by infectious bronchitis virus. i. endemic (balkan) nephropathy. 1977196485
comparative ultrastructural studies on endemic (balkan) nephropathy and chicken embryo nephritis caused by infectious bronchitis virus. ii. chick embryo nephritis. 1977196486
genome of infectious bronchitis virus.techniques are described for the growth and rapid purification of the avian coronavirus infectious bronchitis virus (ibv). purified ibv has a sedimentation coefficient of 320s and a buoyant density of 1.22 g/ml in sucrose-deuterium oxide equilibrium gradients. ibv rna extracted by proteinase k in the presence of sodium dodecyl sulfate and further purified by phenol extraction and gradient centrifugation is single stranded and has a sedimentation coefficient of 64s, as determined by isokinetic gr ...1977198590
[respiratory diseases in poultry caused by viruses--occurrence and diagnosis (author's transl)].the highly intensive conditions, that economic necessity has forced upon the poultry industry, have resulted in strongly changed environmental conditions and management which combined with the use of a constantly increasing number of live vaccines has highly complicated clearing up the etiology in diseased flocks. this is true not least as far as respiratory diseases concerns, which thereby often run an atypical course. a review, however, not complete, is given of the occurrence and diagnostic p ...1977198739
[sensitivity of japanese quail, embryos and embryonal tissue culture to infection with several avian viruses].the sensitivity of the biological system japanese quail-embryo-quail fibroblast cultures to some common fowl viruses (newcastle disease virus, infectious laryngotracheitis and infectious bronchitis viruses) was studied. japanese quails, their embryos and embryo cell cultures were found to be sensitive to newcastle disease virus infection. the virus reproduction was accompanied by death of embryos and destruction of cell culture. japanese quails are sensitive to infectious laryngotracheitis virus ...1977198966
studies on the structure of a coronavirus-avian infectious bronchitis virus.when avian infectious bronchitis virus (ibv) is fixed in formaldehyde, negative stain is able to penetrate the particle and an internal component is visualized. this component is seen as a tongue or flask shaped structure attached at one point to the outer virus membrane. a model yielding transmission patterns similar to the virus has been made. gradient centrifugation studies on ibv reveal that the rnp is associated with the internal sac.1977199696
biological properties of avian coronavirus rna.rna with a sedimentation coefficient of 64s was isolated from infectious bronchitis virus, an avian coronavirus. the sna contained a polyadenylic acid tract and was found to be infectious.1977199697
the polypeptide composition of avain infectious bronchitis virus particles.egg grown avian infectious bronchitis virus (ibv) centrifuged on sucrose density gradients was found to consist of a major virus peak of density 1.17 to 1.18 g/cm(3). three different ibv strains were examined and no morphological differences were detected between virus particles of different densities or from different strains. the polypeptides of the different density virus particles from the three ibv strains were analysed on polyacrylamide gels. in all cases 7 polypeptides were observed, alth ...1977200202
[economic effectiveness of specific preventive measures against infectious bronchitis of chickens]. 1977201081
small infective material coexistent with, but distinct from, avian infectious bronchitis virus. 1977201199
polypeptides of the surface projections and the ribonucleoprotein of avian infectious bronchitis virus.purified avian infectious bronchitis virus was digested with bromelain (0.7 mg/ml), and the surface projections were removed. polyacrylamide gel electrophoresis of the polypeptides from these bromelain-treated particles showed that vp1, vp2, and vp5 were missing from the seven polypeptides. vp1 to vp7, that were present in untreated virus preparations. milder bromelain treatment (0.07 mg/ml) left visible surface projections and polypeptides comprising vp1 and vp2 intact, but removed vp5. thus, t ...1977201778
isolation of avian infectious bronchitis virus from experimentally infected chickens.virus was recovered from the faeces of chickens infected at three or four weeks of age for more than 20 weeks after infection with commercial vaccines or with the t strain of avian infectious bronchitis virus (ibv). virus was not recovered from the trachea, liver, spleen, bursa or kidneys of t strain infected birds longer than 29 days after infection at which point ibv was recovered from the bursa of a single infected bird. in a subsequent experiment ibv was recovered from the caecal lymph nodes ...1977203987
a study of two avian adenovirus serotypes alone or in combination with avian infectious bronchitis in day-old chicks. 1977190275
gumboro disease and infectious bronchitis. 1977190759
effect of infectious bursal disease on the response of chickens to mycoplasma synoviae, newcastle disease virus, and infectious bronchitis virus.at 35 days of age, chickens which as 1-day-old chicks were inoculated with the infectious bursal disease virus (ibdv) had significantly lower antibody titers against mycoplasma synoviae, newcastle disease virus, and infectious bronchitis virus than did those never inoculated with ibdv. the ibdv also had a marked effect on the development of air-sac lesions. birds infected with ibdv that were later inoculated with m synoviae (day 14), newcastle disease virus (days 14 and 28) experienced an increa ...1977190926
correlation of cytopathic effect, fluorescent-antibody microneutralization, and plaque reduction test results for determining avian infectious bronchitis virus antibodies.a microneutralization fluorescent-antibody (mfa) test was effective in determining the level of antibodies to avian infectious bronchitis virus. a comparison of the mfa test with the cytopathic effect microneutralization (mnt) test and 50% plaque reduction (pr) test resulted in positive correlations that were significant (p less than 0.001). the pr test was more sensitive than either the mfa or the mnt test, but there was no significant difference between the sensitivities of the mfa and mnt tes ...1977192758
presence of infectious polyadenylated rna in coronavirus avian bronchitis virus. 1977193262
the characterisation of the virion rna of avian infectious bronchitis virus. 1977193735
[differentiation of type d virus from continuous human cells (il'in-bykovskiÄ­ virus) and mason-pfizer monkey virus by the antigens of the viral envelopes].for differentiation of ilvin-bykovsky virus (ibv) and monkey meson-pfeizer virus (m-pmv) the method of virus neutralization with antibodies against the envelope virus antigen was used. the viruses were cultivated in similar human embryo cells. the results of the virus neutralization were determined by presence or absence of the gs-antigen in the infected cells. the antiserum to m-pmv envelope antigens did not neutralize the ibv antigen. it has been concluded that ibv and m-pmv differ by their en ...1977409444
[infectious bronchitis and colibacteriosis in chickens]. 1976194387
[infectious bronchitis of chickens in azerbaijan]. 1976194388
avian infectious bronchitis vaccine: primordial or derived virus?vaccine formulations of virulent, modified/attenuated, or inactivated mono- or multivalent types of chicken embryo-propagated avian infectious bronchitis virus (ibv) at different passage levels and administered by non-parenteral routes have been used for immunization of chickens against the disease. two doses of vaccines are generally used: 1) high level virus of lessened virulence, antigenicity and immunogenicity as "derived" from its parental virus via serial passage in and selective adaptatio ...197660263
some immunological aspects of a recent australian isolate of infectious bronchitis virus.an infectious bronchitis virus, designated g48, isolated from birds during an outbreak of nephritis in a previously vaccinated broiler flock, overcame the resistance induced in birds vaccinated with 2 commercially available vaccines. birds vaccinated with the a isolate of infectious bronchitis resisted challenge with this new virus. cross neutralisation studies revealed that the new virus was serologically distinct from the 4 viruses tested. homologous antiserum to g48 did not neutralise the oth ...197662570
[studies on the serological differentiation of field virus isolates and the protective action of vaccines in infectious bronchitis]. 1976174542
comparison of a microneutralization test in cell culture and virus neutralization test in embryonated eggs for determining infectious bronchitis virus antibodies.a microneutralization test (mnt) system utilizing cytopathic effect end points was effective in determing neutralization indexes for infectious bronchitis virus antibodies. the system is reproducible within 1 index unit at the 95% level of probability. comparison of the mnt to tests in eggs resulted in a positive correlation (b =0.81), which was significant (p greater than 0.01). the quantitative dose-response relationship of the mnt is linear (p greater than 0.005), with the 95% prediction limi ...1976176174
the use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virus.a study has been made of the use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis (aib) virus from both naturally and experimentally infected chickens. six strains of aib virus were investigated, 3 of which had been isolated from natural outbreaks of disease. two of the virus isolations from the outbreaks of aib were made directly into tracheal organ cultures without passage in embryonated eggs. organ cultures prepared from 20-day-old embryos were use ...1976176965
heterogeneity of infectious bronchitis virus grown in eggs.egg-grown infectious bronchitis virus, strain beaudette, was concentrated and centrifuged on sucrose density gradients to separate the virus into five peaks with densities of 1.144, 1.160, 1.172, 1.191 and 1.218 g/cm3. all peaks retained infectivity, complement fixation activity and were labelled with 3h-uridine. morphologically the densest peak consisted of very large virus particles and amorphous material, the other peaks consisted of mainly intact particles although small differences in size ...1976176969
identification of seven isolants of infectious bronchitis virus by interference with the b-1 isolant of newcastle disease virus.seven isolants of infectious bronchitis virus (ibv) interfered with the development of hemagglutinins (ha) by the b-1 isolant of newcaslte disease virus (ndv) in eggs previously inoculated with these ibv isolants. the i50 [defined as the minimum amount of ibv needed to produce, upon inoculation in embryonating chicken eggs (ece), ha interference in 50% of these ece reioculated 10 hr later with 10(2,41 eid50 (ha) of the b-1 strain of ndv] was less than 1 eid50. the interference was considered spe ...1976176988
new strains of infectious bronchitis virus isolated in maine.twelve ibv isolants from diseased chickens in maine were studied by serum-neutralization tests in chicken tracheal culture. six isolants were neutralized by mass-type antisera. the serum-neutralizing characteristics of six other isolants from vaccine breaks differed from any of the standard ibv viruses and appear to comprise three new strains, maine 209, 212,and 246. the data provide additional evidence that strains of ibv responsible for disease probably vary from region to region and that the ...1976176989
cross-protection studies with a holland strain (noblis h-52) of infectious bronchitis virus. 1976176990
immunity to infectious bronchitis virus from spray vaccination with derivatives of a holland strain.study was made of the protection of chickens from spray vaccination with different modifications of a holland (h) strain of infectious bronchitis virus (ibv). noted in each trial were the virus-serum-neutralizing antibody titers induced and the virulence of the vaccine virus at different passage levels. protection against respiratory signs was good from challenge with a homologous virus serotype as well as with 5 heterologous ibv isolates. this occurred from vaccinating day-old chicks possessing ...1976176991
the isolation and some biological properties of a variant avian infectious bronchitis virus.a virus isolated from a respiratory disease in chickens was identified as infectious bronchitis virus (ibv). by serum neutralisation tests the virus was shown to be serologically distinct from the massachusetts type but to possess a one-way relationship with the connecticut type. experimentally, it caused a relatively mild respiratory disease in baby chicks. vaccination of young chicks with h-120 massachusetts ibv vaccine afforded a high degree of protection against challenge with the isolate. s ...1976179183
growth kinetic studies of avian infectious bronchitis virus in tracheal organ cultures.an egg-adapted vaccine strain (h120) and an organ culture-passaged field strain (hv-10) of avian infectious bronchitis (aib) virus were propagated in tracheal organ cultures and their growth kinetics examined using nine-day-old embryonated fowl eggs and chick tracheal explants for virus assay. when the h120 strain was assayed in embryonated eggs, titres were approximately log10 2-0 id50 (50 per cent infectious dose) per ml higher than when assays were performed in tracheal explants. the hv-10 st ...1976180581
vaccination and revaccination with a holland (h) strain of infectious bronchitis virus.one-day-old chickens possessing maternally derived immunity to massachusetts-type infectious bronchitis virus (ibv) were vaccinated by the eyedrop route with a holland (h) ibv strain of the massachusetts type. vaccination was done with the 72nd and 92nd passages in chicken embryos. attenuation of the virus was done in the authors' laboratory. four weeks postvaccination the immunity was challenged with 8 different ibv serotypes. a broad spectrum of protection from respiratory signs was obtained i ...1976180959
strains of infectious bronchitis virus on the delmarva peninsula and in arkansas.the constant-virus variable-serum neutralization test in chicken tracheal cultures was used to serotype infectious bronchitis virus (ibv) isolants from the delmarva peninsula and arkansas. of 106 isolants obtained from delmarva in 1971-1974, 93 were found to be the jmk strain, 5 massachusetts (mass), 5 connecticut (conn), 2 a new strain designated maryland (md) 27, and 1 a new strain designated md 31. of 26 isolants obtained from arkansas between october, 1971, and february, 1973, 17 were arkans ...1976180960
variant infectious bronchitis virus isolated from indiana chickens.infectious bronchitis virus (ibv) associated with a catarrhal tracheitis, sudden decline in egg production, and reduced shell quality was isolated from an indiana white leghorn breeder flock. it was found to be serologically different from massachusetts, connecticut, iowa 97, iowa 609, florida, arkansas 99, jmk, holte, gray and se 17 ibv serotypes. two different massachusetts vaccine strains protected chickens from respiratory signs but not against virus infection using the isolant for challenge ...1976181740
letter: protection by ibv vaccine. 1976181893
local immunity in newcastle disease: some recent experiments.following primary exposure by the ocular route to lentogenic newcastle disease virus (ndv) the lacrymal fluid, saliva and tracheal washes of three-week old specific pathogen free chickens acquired specific virus-neutralizing activity which considerably exceeded transudation of circulating antibody. all three secretions contain iga which, at least in saliva, accounted for 85% of its activity, the remanider being due to igg. antibody in secretions limited, but did not prevent, reinfection of the ...1976182586
the proposed international standard for infectious bronchitis vaccine (live).an h-120 strain of infectious bronchitis virus was prepared in a form suitable to serve as an international standard for infectious bronchitis vaccine (live). accelerated degradation studies carried out on this vaccine showed it to be of suitable stability to serve as a standard. results from a collaborative assay involving ten laboratories demonstrated that the proposed standard reduced the variation in virus titrations both within and between laboratories. this assay also showed that firstly ...1976182587
comparative tests on safety and potency of iba vaccines.during a comparative test on the pathogenicity and immunogenicity of a non-adapted and an embryo-adapted ida virus, following observations were made: 1) the non-adapted virus remained pathogenic as determined by weight loss and bursal lesions. clinical signs and mortality did not occur. a change in the virulence did not occur during back passages; 2) the immunosuppressive effect of the non-adapted virus was diminished by maternal antibodies; 3) the embryo-adapted virus produced little pathogenic ...1976182590
[the influence of infectious bronchitis virus on egg production, fertility, hatchability and mortality rate in chickens (author's transl)].in 35 flocks comprising almost 100,000 white plymouth rock birds producing hatching eggs, infectious bronchitis (ib) caused a decline in production which appeared to be connected with the age of the birds, when the outbreak started (table i). in outbreaks of ib in pullets 3-4 weeks before they are ready to lay there is a considerable risk of delay of production and a generally too low yield (fig 1). egg laying birds, 25-34 weeks of age, suffered a drop in production from 20-80% (on an average 40 ...1976183182
serologic differences between strains of infectious bronchitis virus from new zealand, australia, and the united states.the antigenic relationships of strains of infectious bronchitis virus from new zealand, australia, and the united states were evaluated by a virus-neutralization procedure similar to that used in typing human rhinoviruses. the four previously described new zealand serotypes were antigenically distinct from all the other strains tested.1976183648
isolation of infectious bronchitis virus from broiler chickens in chile.an infectious-bronchitis-like virus was isolated from 6-week-old broiler chickens unvaccinated with infectious bronchitis virus (ibv). the isolate produced lesions in embryonating chicken eggs (ece) and clinical signs in birds characteristic of ibv. serum-neutralization and agar-gel diffusion tests demonstrated the serological relationship of this isolate to ibv.1976183652
organ culture studies on the efficiency of infection of chicken tissues with avian infectious bronchitis virus.long-term organ cultures of a range of tissues collected from specific pathogen-free chickens were employed to determine their susceptibility, and their capacity for subsequent virus production, following inoculation with avian infectious bronchitis (aib) virus. when inoculated with approximately 2-0 log10 median ciliostatic doses (cd50) of a classical highly egg-adapted vaccine strain (h120) of aib virus, 9 of 23 tissues were shown to be susceptible, namely the nasal turbinates, trachea, air sa ...1976183805
the histopathology of infectious bronchitis in fowls infected with a nephrotropic "t" strain of virus.the histopathology of the infectious bronchitis caused by the cumming "t" strain of virus is described in fowls exposed to infection by an aerosol method. desquamation of the ciliated and glandular epithelium throughout the trachea was seen 24 hours after exposure to virus. this was followed by rapid proliferation presumably of residual basal cells with the production of a stratified undifferentiated epithelial covering. small areas of the tracheal submucosa showed lymphocytic infiltration by th ...1976186001
morphogenesis of avian infectious bronchitis virus in primary chick kidney cells.primary chick kidney cells were infected with avian infectious bronchitis virus (ibv) and examined by electron microscopy. virus particles entered the cells by viropexis and distinction could be made between engulfment by cell processes (phagocytosis) and entry by micropinocytosis in coated transport vesicles. virus maturation occurred by budding into either the cisternae of the endoplasmic reticulum or cytoplasmic vacuoles, and evidence was obtained to suggest that the viral surface projections ...1976187144
electron microscope observations on the entry of avian infectious bronchitis virus into susceptible cells.infectious bronchitis virus was observed to enter cells of chicken chorioallantoic membrane by viropexis. there was no support for the suggestion that entry took place by fusion of viral and plasma membranes. the results of electron microscopy showed that virus attachment occurred both at 4 degrees and at 37 degrees c. viropexis was not observed until the preparations were warmed. similar results were obtained using chicken kidney cells. quantitative data obtained from a plaque counting system e ...1976187152
immunosuppressive effects of the infectious bursal agent and relationships to other poultry diseases. 1976220631
local immunity in the respiratory tract of the chicken. i. transudation of circulating antibody in normal and virus-infected birds.three-week-old chickens were given sheep erythrocytes or bovine serum albumin intravenously. seven days later their tears and saliva possessed low levels of antibody to those antigens. concurrent infection with lentogenic newcastle disease virus (ndv) caused a significant increase in transuded antibody in those fluids. in chickens with circulating antibody to ndv, induced by parenterally administered inactivated vaccine, respiratory infection with heterologous infectious bronchitis virus resulte ...19761027720
stability of live, freeze-dried virus vaccines.stability of live, freeze-dried vaccines is a major factor for successful vaccinations. vaccines may be stored at -20 degrees c or less for long periods without loss of activity and this storage does not affect stability during subsequent storage. storage in the refrigerator for long periods is harmless for certain very stable vaccines such as influenza, rubella, ndv, marek's. other vaccines, such as ibv, show a significant loss in titer after 2 years. the routine use of an accelerated stability ...19761030427
changes in the harderian gland of the chicken following conjunctival and intranasal infection with infectious bronchitis virus in one- and 20-day-old chickens.three days after conjunctival and intranasal infection with ib vaccine virus a great increase in the number of plasma cells together with an increase in vascularization was observed in the stroma of the harderian gland of chickens vaccinated either at one day of age or 20 days old. from 7 days to 21 days after vaccination a pronounced formation of follicles occurred which were composed of lymphocytes. birds exposed to diluted allantoic fluid showed a limited increase of plasma cells only; follic ...197618777328
preliminary evaluation of the haemagglutination and haemagglutination inhibition tests for avian infectious bronchitis virus.four of the 9 strains of infectious bronchitis virus which were concentrated and treated with phospholipase c type 1 showed haemagglutination activity. these strains, holte, massachusetts 41 (m41), h120 and connecticut, were distinguishable by the haemagglutination inhibition (hi) test but showed much closer relationships than could be detected by the plaque reduction (pr) test. the four haemagglutinating strains were used to compare the hi and pr titres of 17 anti sera prepared against referenc ...197618777337
observations on the effects of the h52 and h120 vaccine strains of infectious bronchitis virus in the domestic fowl.the pathogenicity of h120 and h52 infectious bronchitis vaccine viruses was compared in one-day-old and 12-weeks-old chickens. both viruses produced moderate respiratory symptoms. both were also nephrotropic but whereas h52 was accompanied by gross and microscopic kidney changes, which probably accounted for the mortality which occurred, h120 produced no change in the kidney and no mortality. both viruses caused gross and microscopic changes in the bursa of fabricius.197618777342
dropped egg production, soft shelled and shell-less eggs associated with appearance of precipitins to adenovirus in flocks of laying fowls.two outbreaks of dropped egg production and production of soft shelled and shell-less eggs are described. the outbreaks were selected from a larger number of flocks with similar problems in the field. the drop in egg production was closely correlated with the appearance of precipitins to adenovirus in the laying birds. birds were also infected with mycoplasma synoviae. a number of other infectious causes of production problems could be excluded in these outbreaks. in particular infectious bronch ...197618777355
immune response to infectious bronchitis virus. 19751092219
the ribonucleic acid of infectious bronchitis virus.analysis of the nucleic acid of infectious bronchitis virus by sds polyacrylamide gel electrophoresis revealed an rna of molecular weight 9.0 times 10-6 daltons. the rna was shown to have a sedimentation coefficient of 50.19751119945
characterization of a new infectious bronchitis virus isolate. iii. cell-culture adaptation of clark 333.the clark 333 strain of infectious bronchitis virus (ibv) was substantially resistant to primary chicken cell-culture adaptation. more than 40 serial embryo passages were required before the virus would produce cytopathic alterations upon cell-culture inoculation. the cytopathic effect was characteristic of the effect of reported for ibv. adaptation was not accomplished by alternating serial passages in embryo and cell-cultured systems. a careful monitoring of cell-culture fluid infectivity by e ...19751120038
the neutralizing characteristics of strains of infectious bronchitis virus as measured by the constant-virus variable-serum method in chicken tracheal cultures.the constant-virus variable-serum neutralization test in chicken tracheal organ cultures was employed in cross-neutralization tests with the following infectious bronchitis viruses: massachusetts 41, connecticut, iowa 97, iowa 609, holte, jmk, clark 333, se 17, florida, and arkansas 99. these viruses all proved serologically distinct by this method. chickens immunized with the connecticut strain and later challenged with arkansas 99 were not protected against it.19751120040
single and mixed infections of avian infectious bronchitis virus and mycoplasma gallisepticum.comparative studies of the clinical signs, pathological changes, multiplication of the pathogens and serological responses were made of groups of chickens infected with either or both avian infectious bronchitis virus and mycoplasma gallisepticum. electron microscopic examination of thin sections of infected tracheas showed that the multiplication of m. gallisepticum was greatly enhanced in the tracheas of chickens which had been previously or simultaneously infected with avian infectious bronch ...19751126565
ph stability studies with avian infectious bronchitis virus (coronavirus) strains.a comparison of 17 infectious bronchitis virus strains, using the same test procedure and assay system, demonstrated that stability at an acid ph is a variable characteristic of the avian coronaviruses.1975234539
immunity to avian infectious bronchitis.avian infectious bronchitis is recognized clinically as a respiratory disease in its only natural host,the chicken, but the virus is disseminated throughout other systems by a viraemia with localization especially in the kidney and oviduct. the sensitivity or instability of the antigenic and immunogenic properties of the virus under laboratory stress or natural influences complicates the selection of seed virus for vaccines. modified or attenuated active virus vaccines induce greater protection ...1975805074
in vitro cultivation of cells from the chorioallantoic membrane of chick embryos.by treatment of chorioallantoic membranes from embryonated eggs with collagenase and hyaluronidase before the conventional application of trypsin cells could be grown in culture which supported growth of a large variety of myxoviruses, herpesviruses, avian reoviruses and the infectious bronchitis virus of chickens. the cultures could be used for sensitive plaque assays and neutralization tests.1975165393
effect of infectious bronchitis vaccination on the performance of live newcastle disease vaccine. 1975166478
use of the indirect fluorescent antibody method for detecting antibodies to infectious bronchitis virus in chickens serum. 1975167061
letter: infectious bronchitis in vaccinated poultry. 1975167505
serological responses of broiler-type chickens, with and without newcastle disease and infectious bronchitis vaccine, to experimental infection with mycoplasma synoviae by foot pad, air sac and aerosol.serum plate (sp) and tube agglutination (ta) reactions and geometric mean mycoplasma synoviae (ms.) hemagglutination-inhibition (hi) titers were determined on chickens infected at 21 days of age with ms. by foot pad, air sac and aerosol. one-half had been given newcastle disease (nd) and infectious bronchitis (ib) vaccine in the water at 20 days of age, and the other half had not been vaccinated with nd and ib vaccine. blood was taken at 3, 4, 6 and 8 weeks after ms. infection. geometric mean hi ...1975168564
two new isolants of infectious bronchitis virus with polyvalent immunogenicity.the immunizing capacity of two new field isolants of avian infectious bronchitis virus (ibv) was tested in vivo. chickens vaccinated with either isolant resisted challenge with 6 serotypes of ibv, including some of the types most commonly isolated from chickens. the two new isolants, identified herein as california "g" and california "s," appear to be immunologically different from each other and from the other 6 types tested. the polyvalent immunogenicity of both g and s is noteworthy, and pote ...1975168851
identification of infectious bronchitis virus by interference with the b-1 isolant of newcastle disease virus. waxing and waning of interference.the massachusetts and the connecticut types of infectious bronchitis virus (ibv) were identified by interference in embryonating chicken eggs (ece) with the production of hemagglutinin by the b-1 isolant of newcastle disease virus (ndv). this interference test appears to be specific because the above interference was eliminated by adding type-specific anti-ibv serum to the ibv-ndv system; however, interference was not detectable when fowlpox virus (fpv) and infectious laryngotracheitis virus (lt ...1975168852
morphological changes of chicken tracheas and tracheal organ cultures infected with avian infectious bronchitis virus studied in scanning electron microscope.chicken tracheas infected in vitro in organ culture and in vivo with avian infectious bronchitis virus (ibv) were studied by scanning electron microscopy. in in vitro infection in organ culture the columnar epithelial cells lining the tracheal ring lost their cilia and rounded up in 48 hr. there were numerous elevations and holes on the infected cell surface. subsequently the cells sloughed off. in in vivo infection the tracheal epithelial cells lost their cilia in 4 days. these cells without ci ...1975169800
serotyping of avian infectious bronchitis viruses by the virus-neutralization test.the antigenic relationships of 12 strains of infectious bronchitis virus (ibv) were evaluated by a virus-neutralization procedure similar to that used in typing human rhinoviruses. such a procedure consists of reciprocal neutralization tests performed by reacting 32-320 eid50 or plaque-forming units of virus with 20 antibody units of antiserum. eight serologic groups were identified by chicken embryo assay, and 4 by plaque-reduction (90%). in general, serologic groupings were not distinct but re ...1975169804
haemagglutination by avian infectious bronchitis virus-a coronavirus.the haemagglutinating ability of three strains of ibv was investigated. it was shown that whereas strain beaudette had no detectable haemagglutinin, both connecticut and massachusetts agglutinated red cells of various species. the haemagglutinin of connecticut was detectable after sucrose gradient purification whereas that of massachusetts required both the purification step and incubation with the enzyme phospholipase c to reveal it. the agglutination could be inhibited by specific antisera. so ...1975170378
vaccination of young chickens with live vaccine against some new zealand isolates of infectious bronchitis virus. 1975170569
comparative growth kinetic studies on avian infectious bronchitis virus in different systems. 1975171289
the polypeptide composition of avian infectious bronchitis virus.avian infectious bronchitis virus grown in ovo was purified by differential centrifugation and isopycnic sedimentation in density gradients. the purified virus was analysed by sds polyacrylamide gel electrophoresis and found to comprise up to sixteen polypeptides, four of which were glycopeptides. bromelain treatment of the particles removed three polypeptides and two glycopeptides.1975174524
effect of ph on the growth and cytopathogenicity of avian infectious bronchitis virus in chick kidney cells.the growth of avian infectious bronchitis virus (ibv) in chick kidney cells at different ph values in the range 6.0-9.0 demonstrated that although the virus was released at a much faster rate at the higher ph values the titre tended to drop more quickly. at the acid ph values the virus was released more slowly but reached a maximum titre similar to that at the higher ph values and showed only minimum reduction in infectivity up to 49 hours post inoculation. the stability of virus in tissue cultu ...19752145
avian infectious bronchitis: characteristics of the virus and antigenic types. 197547725
potential for polyvalent infectious bronchitis vaccines.numerous antigenic types and subtypes of infectious bronchitis virus (ibv) have been isolated and characterized from field epornitics of infectious bronchitis (ib). thus obvious failures in obtaining the necessary protection from vaccination have been documented and they underscore the urgent need for vaccines which stimulate a broader spectrum of protection from heterologous ibv challenge than is presently obtained. if polyvalent ib vaccines are to be employed, assurance must be provided that v ...197547726
[differentiation of haemophilus strains isolated from chickens. iii. pathogenicity tests in chickens].pathogenicity tests of 6 isolates of the haemophilus-group i (h. paragallinarum) and 8 isolates of the haemophilus-group ii isolated from chickens in the federal german republic and one strain of h. parainfluenzae isolated from man were carried out in 6-week old spf-chickens. infectious coryza (coryza contagiosa gallinarum) could be produced in chickens with all strains of group i after experimental inoculation and by contact exposure. at necropsy 3 weeks post inoculation (p.i.) organisms of gro ...197518777310
some observations on the propagation of avian infectious bronchitis virus in tissue culture. 19744207765
neutralizing and precipitating antibodies against infectious bronchitis virus in chickens. 19744208959
avian infectious bronchitis: demonstration of serum igg- and igm-neutralizing antibody by sucrose density-gradient centrifugation and mercaptoethanol reduction. 19744215411
local immunity to avian infectious bronchitis in tracheal organ culture. 19744368876
isolation of avian viruses from the eggs of raillietina tetragona and ascaridia galli. 19744376077
the effect of the suppression of circulating antibody on resistance to the australian avian infectious bronchitis virus. 19744419569
interferon induction by different strains of infectious bronchitis virus. 19744423767
nephrosis in laying chickens caused by massachusetts-type infectious bronchitis virus. 19744450393
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