Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [role of metal ions in the catalytic activity of aspergillus oryzae aminopeptidase]. | the paper deals with the role of metals in the catalytic action of asp. oryzae aminopeptidase. cobalt ions are more specific activators than mn2+ and mn2+ and evoke its maximal activity. sinergic activation of co2+ in combination with mn2+ and mg2+ was not found in contrast to some aminopeptidases of animal origin. activation of the enzyme with cobalt chloride depends on temperature. by the 10th minute of incubation the activation reaches its maximum: 650 and 900% at 20 and 40 degrees c, respect ... | 1979 | 106499 |
| aflatoxin produced by five species of aspergillus on rice. | 1979 | 114865 | |
| differential excision from dna of the c-8 deoxyguanosine reaction products of n-hydroxy-2-aminofluorene and n-acetoxy-n-acetyl-2-aminofluorene by endonuclease s1 from aspergillus oryzae. | calf thymus dna was modified in vitro with [g-3h]n-hydroxy-2-aminofluorene and [g-3h]n-acetoxy-n-acetyl-2-aminofluorene and the nuclease s1 digestion was studied under identical conditions. the ratios of the maximum reaction rate (v) and the michaelis constant (km), v/km, indicate that 2-aminofluorene(af)-modified dna is hydrolyzed 3 times more slowly than n-acetyl-2-aminofluorene(aaf)-modified dna under similar reaction conditions. the af-modified dna was slightly more susceptible to partial di ... | 1979 | 476609 |
| [kinetic parameters of superhelical dna cleavage by endonuclease s1]. | major kinetic parameters of endonuclease s1 were determined on superhelical bacteriophage pm2 dna and on relaxed nicked circular pm2 dna. at 37 degrees and 0,25 m nacl, the michaelis constants were respectively 1.7 . 10(-8) m and 1 . 10(-9) m, and catalytic constants were respectively 0.36 sec-1 and 1.2 . 10(-2) sec-1. the inhibition of the enzyme reaction by its product was detected. | 1979 | 503058 |
| [immunological studies on allergic bronchopulmonary aspergillosis--probably induced by aspergillus oryzae (author's transl)]. | 1979 | 541917 | |
| [formation of volutin inclusions in the mycelium of aspergilli growing on media with hydrocarbons]. | 1979 | 440149 | |
| [nuclease from aspergillus oryzae, specific for single-stranded regions of nucleic acids]. | a single-stranded specific nuclease has been purified from amyloryzine obtained from the mould fungi aspergillus cryzae. the nuclease under study resembles the enzymes described in the literature in its ability to hydrolyze single-stranded nucleic acids. however, the enzyme essentially differs from previously known nucleases in some catalytic properties, particularly in its ability for degradation of poly a. it has been shown that the enzyme also hydrolyzes the synthetic dinucleotide ptpt to mon ... | 1979 | 465607 |
| [isolation and some properties of homogenous nuclease s1 from alpha-amyloryzin]. | a purification method for isolating homogeneous single-strand specific nuclease s1 from alpha-amyloryzin has been developed. the yield was about 16% and purification factor--9000. nuclease s1 thus obtained was proved to be free of contaminations of any others nucleolytic enzymes. it is shown for the first time that ribo- and deoxy-dinucleosidemonophosphates are hydrolyzed by nuclease s1 to form 5'-nucleotides with ph optimum for apa equal to 4.6. | 1979 | 547181 |
| studies on the inhibitory effects of zinc heptanoate on microorganisms. | inhibitory effect of zinc heptanoate was observed on different cultures of bacteria and fungi. growth of all the bacteria was inhibited by the compound. greatest inhibition was seen in the case of staphylococcus albus, streptococcus pyogenes, shigella dysenteriae, shigella sonnei, shigella flexneri, salmonella typhi, s. paratyphi a, s. paratyphi b, vibrio cholerae, corynebacterium diphtheriae, and e. coli whereas least inhibition was found in the case of staphylococcus aureus. in triethanolamine ... | 1979 | 553835 |
| multimolecular substrate reactions catalyzed by caabohydrases. aspergillus oryzae alpha-amylase degradation of maltooligosaccharides. | aspergillus oryzae alpha-amylase degrades maltooligosaccharides by other pathways besides simple glycosidic bond scission. the utilization of the alternate pathways increases with the concentration of substrate implicating a multimolecular substrate mechanism. reducing-end labeled and uniformly labeled maltooligosaccharides were used to elucidate these alternate degradation mechanisms. condensation followed by hydrolysis is not a significant pathway. transglycosylation is concluded to occur, but ... | 1978 | 307963 |
| mapping of deoxydi- and trinucleotides liberated by the action of endonucleases from the silkworm and aspergillis oryzae. | both silkworm nuclease and nuclease o of aspergillus oryzae mycelia hydrolyse dna endolytically to di- and trinucleotides terminating in 5'-phosphate. these oligonucleotides were fractionated first be deae-cellulose chromatography with 7 m urea into the respective isoplithic groups and then analysed for the composition and isomerism: each group was labeled 5'-terminally by the [gamma-32p]atp-polynucleotide kinase reaction and then electrophoresed monodimensionally for the dinucleotides and two-d ... | 1978 | 656423 |
| [role of cyclobutane pyrimidine dimers in uv-denaturation of dna]. | 1978 | 739994 | |
| the influence of charged matrix surfaces on the thermostabilizing effect of calcium ions on immobilized fungal alpha-amylase. | the stabilizing effect of calcium ions on fungal alpha-amylase (ec 3.2.1.1) immobilized on a polystyrene anion exchanger (p+ amylase) was investigated and compared to the behaviour of soluble amylase. moreover, gamma-(1,4-benzoquinone-2-yl)-aminopropyl silica-amylase (si(n) amylase) as a conjugate with weakly basic amino groups and gamma-succinamidopropyl silica amylase (si- amylase) as a conjugate with free carboxyl groups were applied for comparison. depending on the calcium ion concentration ... | 1978 | 749472 |
| rate equation for amylase-catalyzed hydrolysis, transglycosylation and condensation of linear oligosaccharides and amylose. | 1978 | 632229 | |
| induction and repair of strand breaks and 3'-hydroxy terminals in the dna of mouse brain following gamma irradiation. | dna was isolated from mouse brain after in vivo gamma-ray irradiation, treated with endonuclease s1 from aspergillus oryzae if necessary, and analysed further by alkaline and neutral sucrose gradient centrifugation. in parallel, its template activity was determined by dna polymerase (ec 2.7.7.7, enzyme a of klenow from escherichia coli) assay as described previously. similar experiments were performed with cultured mouse leukaemia cells (l5178y) irradiated in vitro at 0 degrees c. irradiation in ... | 1978 | 718924 |
| specific site of action for single-strand specific nuclease on the double-stranded circular dna intermediates of an avian rna tumor virus. | circular viral dna intermediates obtained from the quail tumor line, qt6, at 1 day after infection, were opened at one specific location by the single-strand specific nuclease, s1, of aspergillus oryzae. this site was no longer accessible to the s1 nuclease when circles were first opened at another location with a restriction endonuclease. | 1978 | 215777 |
| [production of mutants with an increased alpha-amylase synthesis]. | a mutant characterized by elevated biosynthesis of alpha-amylase was obtained as a result of a three-stage induced selection using nitroso compounds. changes of mutagens in the course of selection stages and the establishment of their effective doses causing the maximum accumulation of mutations yielded the mutant which produced 2.5 times more alpha-amylase than the parent strain of aspergillus oryzae 762. the induced variability of the mutant can be registered on a solid growth medium and provi ... | 1978 | 309059 |
| repetitive attack by aspergillus oryzae alpha amylase. | the action of aspergillus oryzae alpha amylase on reducing-end, and uniformly radiolabeled maltotriose through maltodecaose has been studied. the enzyme is found to hydrolyze more than a single glycosidic bond during enzyme-substrate encounters. the extent of this repetitive attack is quantitated. | 1978 | 306286 |
| [alpha-amylase activity in lysosomes of aspergillus oryzae (author's transl)]. | the alpha-amylase of mycelial cells of aspergillus oryzae exists in a particular form in 8000 g pellet. the lysosomal localization of acid phosphatase is confirmed by electron microscopy. the purification of lysosomes by discontinuous gradient of sucrose in d2o shows that alpha-amylase activity is bound to these particles. | 1978 | 306932 |
| difference spectroscopic study of the interaction between taka-amylase a and substrates. | 1978 | 306996 | |
| studies on the substrate specificity of taka-amylase a. xiii. preparation of 6-deoxy-6-iodomaltooligosaccharides and their inhibitory action against taka-amylase a1. | o-alpha-d-glucopyranosyl-(1 leads to 4)-o-6-deoxy-6-iodo-alpha-d-glucopyranosyl-(1 leads to 4)-d-glucopyranose (6'-mt), o-alpha-d-glucopyranosyl-(1 leads to 4)-6-deoxy-6-iodo-d-glucopyranose (6-m), and o-6-deoxy-6-iodo-alpha-d-glucopyranosyl-(1 leads to 4)-d-glucopyranose (6'-m) were prepared and their inhibitory action against taka-amylase a [ec 3.2.1.1, alpha-1, 4-glucan 4-glucanohydrolase, aspergillus oryzae] was investigated. the inhibitor constants of 6'-mt and 6'-m were 10 mm and 54 mm, re ... | 1978 | 306997 |
| model for carbohydrase action. aspergillus oryzae alpha-amylase degradation of maltotriose. | aspergillus oryzae alpha-amylase catalyzes degradation of oligosaccharides by a variety of pathways. we present here a quantitative study of the degradation of maltotriose by this amylase. our results lead to a scheme involving multiple transglycosylation reactions and shifted binding due to simultaneous binding of two substrate molecules. the scheme is able to account for the diverse body of information collected for the enzyme. the effect of substrate concentration on the products of maltotrio ... | 1978 | 307964 |
| a study of the mechanism of action of taka-amylase a1 on linear oligosaccharides by product analysis and computer simulation. | the action pattern and mechanism of the taka-amylase a-catalyzed reaction were studied quantitatively and kinetically by product analysis, using a series of maltooligosaccharides from maltotriose (g3) to maltoheptaose (g7) labeled at the reducing end with 14c-glucose. a marked concentration dependency of the product distribution from the end-labeled oligosaccharides was found, especially with g3 and g4 as substrates. the relative cleavage frequency at the first glycosidic bond counting from the ... | 1978 | 308947 |
| [acid-stable and acid-unstable alpha-amylases of the mold fungi aspergillus]. | acid-sable alpha-amylase of asp. niger and acid-unstable, alpha-amylase of asp. oryzae were studied. it was demonstrated, that beside being a more acid-stable properties, alpha-amylase asp. niger has increased thermal stability as compared to alpha-amylase asp. oryzae. the molecular weights of acid-stable alpha-amylase and acid-unstable alpha-amylase are 58 000 and 51 000, respectively. the amino acid composition, and the c- and n-terminal amino acids of both forms of alpha-amylases were determi ... | 1978 | 31203 |
| presence and partial characterization of internal acid protease of aspergillus oryzae. | the presence and partial characterization of the internal acid protease (ec 2.4.23.6) of aspergillus oryzae has been investigated. although the majority of the acid protease is external and present in the culture filtrate, a significant amount of the active enzyme is firmly bound to the cells; it is not released by repeated extraction of cells with 0.9% sodium chloride but is liberated into the soluble fraction during disruption of cells. the internal acid protease, as well as the external one, ... | 1978 | 29561 |
| how much is secondary structure responsible for resistance of double-stranded rna to pancreatic ribonuclease a? | 1. double-stranded f2 sus11 or qbeta rnas, resistant to bovine pancreatic rnaase a in 0.15 m nacl/0.015 m sodium citrate (ssc), are quickly and completely degraded at 10-fold lower ionic strength (0.1 x ssc) under otherwise similar conditions. at this ionic strength the secondary structure of double-stranded rna is maintained, as judged by the following: (a) the unchanged resistance of double-stranded rna and dna, under similar low ionic strength conditions, to nuclease s1 from aspergillus oryza ... | 1978 | 26405 |
| purification and characterization of the two molecular forms of membrane acid protease from aspergillus oryzae. | two forms (m1 and m2) of the membrane-bound acid protease of aspergillus oryzae have been purified by extraction with triton x-100, washing with cold acetone, and repeated gel filtration on bio-gel a-15 m in the presence and absence of triton x-100. the purified membrane enzymes, m1 and m2, moved as a single band in acrylamide gel electrophoresis and had apparent molecular weights of 150 000 and 60 000, respectively, as estimated by sodium dodecyl sulfate/acrylamide gel electrophoresis. these tw ... | 1978 | 25177 |
| immobilization of alpha-amylase on porous glass and silochrome. | immobilized forms of alpha-amylase from aspergillus oryzae were prepared on the porous glass and silochrome by the glutaraldehyde method. an addition of calcium ions at a concentration of 0.05 m to the reaction mixture during immobilization stabilized the enzyme activity. ph optimum of the insoluble form of alpha-amylase was 5.8 and that of the soluble form was 4.7. storage of the insoluble enzyme as water suspension in 0.015 m cacl2 at 4 degrees c for six months and twenty times repeated specif ... | 1978 | 24839 |
| on a rabbit hyperlipemia induced by a fungic galactomannane peptide. | i.v. injection into rabbits of a fungic galactomannane peptide isolated from the culture medium of aspergillus oryzae induced the apparition, 20 h later, of an hypertriglyceridemia, with a concomittant decrease of about 70% of the post-heparin lipoprotein lipase activity. the same effect had been obtained earlier with a carbohydrate-rich fraction purified from a crude papain preparation. the 2 fractions are compared. | 1978 | 738437 |
| properties of limit dextrinase of aspergillus oryzae. | 1978 | 753749 | |
| [experiences with substitution therapy using a new pancreatic enzyme of plant origin]. | the indication field of nortase, a combination of microbial lipolytic and proteolytic enzymes, comprises the replacement therapy of maldigestion and insufficiency of pancreas. its efficacy and tolerance were tested in 100 patients in an open study under the conditions of general practice. during the 15-day treatment the following symptoms were evaluated: anorexia, flatulence, pressure and pain in the epigastrium, nausea after the meals, belching, pyrosis, the quality of feces and the body weight ... | 1978 | 700583 |
| isolation of amylolytic system of aspergillus oryzae on deae amylum. | studying isolation technique with deae amylum the method was found to be suitable for isolation of alpha-amylase. after determination of optimal conditions sorption curves were illustrated, from which the amount of deae amylum needed for 100% enzyme sorption from solutions of various activity and origin can be calculated. preparation obtained shows high temperature stability and can be used in food industry and agriculture or after its elution in pharmacy. at the end of the paper laboratory isol ... | 1977 | 846561 |
| chemical properties of the polysaccharides associated with acid protease of aspergillus oryzae grown on solid bran media. | 1977 | 881411 | |
| s1 nuclease from aspergillus oryzae for the detection of dna damage and repair in the gamma-irradiated intracerebral rat gliosarcoma 9l. | 1977 | 905506 | |
| degradation of nucleic acids in cell lysates by s1 nuclease in the presence of 9 m urea and sodium dodecylsulfate. | single-strand-specific nuclease s1 from aspergillus oryzae is shown to degrade dna and rna in lysates of hela cells in the presence of 9 m urea and sodium dodecylsulfate. free dodecylsulfate inhibits s1 nuclease. however, if the detergent is complexed with proteins prior to the addition of the enzyme, s1 nuclease can degrade nucleic acids at dodecylsulfate concentrations which would inhibit the enzyme completely if no other proteins were present. in lysates prepared from hela cells by treatment ... | 1977 | 908332 |
| specific hydrolysis of rabbit globin messenger rna by s1 nuclease. | s1 nuclease isolated from aspergillus oryzae has been used to investigate the secondary structure of rabbit globin messenger rna (mrna). the enzyme, which is specific for single stranded nucleotides, digests globin mrna to a limited extent, with 65-75% of the mrna nucleotides resistant to digestion under mild conditions. this limited digestion is not due to enzyme inactivation, but rather to the normal activity of the single-strand nuclease. the reaction was studied as a function of temperature, ... | 1977 | 909777 |
| a rapid direct assay for the determination of the separate activities of the three arylsulphatases of aspergillus oryzae. | 1. the three arylsulphatases of aspergillus oryzae exhibit pronounced kinetic differences and substrate specificities. arylsulphatase i hydrolyses all substrates tested, whereas arylsulphatase iii will not hydrolyse tyrosine o-sulphate or phenolphthalein disulphate. arylsulphatase ii does not hydrolyse p-nitrophenyl sulphate or phenolphthalein disulphate at appreciable rates in the absence of added phenolic compounds. phenols such as tyramine increase the rate of hydrolysis of these substances b ... | 1977 | 597235 |
| differential repression of arylsulphatase synthesis in aspergillus oryzae. | 1. the activities of the three arylsulphatases (arylsulphate sulphohydrolase, ec 3.1.6.1) of aspergillus oryzae produced under a variety of repressing and non-repressing conditions were determined. 2. these enzymes exhibit different sensitivities to repression by inorganic sulphate. 3. arylsulphatase i, but not arylsulphatases ii and iii, exhibits a transient de-repression in the early growth phase in sulphate media. 4. when the fungus is cultured in repressing media and subsequently transferred ... | 1977 | 597236 |
| isolation and amino-terminal sequence analysis of a new pancreatic trypsinogen of the african lungfish protopterus aethiopicus. | the purification and characterization of three pancreatic trypsinogens a1, a2, and a3, from the african lungfish, protopterus aethiopicus, is reported. these zymogens are activated by trypsin, by enterokinase, by an acid protease from aspergillus oryzae, and by autoactivation. the three trypsinogens contain the same amino-terminal amino acid sequence, beginning with the activation peptide leu-pro-leu-glu-asp-asp-lys-. like the activation peptide of the previously characterized trypsinogen b [ree ... | 1977 | 911766 |
| natural plant enzyme inhibitors. v. a trypsin/chymotrypsin inhibitor from alocasia macrorhiza tuber. | a trypsin/chymotrypsin inhibitor was isolated from the tubers of alocasia macrorhiza by extraction at ph 7.6, heat treatment at 80 degrees c, ammonium sulphate precipitation and successive column chromatography on cm-cellulose, deae-sephadex a-50 and sephadex g-100. the inhibitor was pure by cellulose acetate electrophoresis. the molecular weight was approximately 32 000 as determined by gel filtration on sephadex g-100. the inhibitor acted on bovine trypsin, human trypsin and bovine chymotrypsi ... | 1977 | 911861 |
| optimal conditions of alpha-amylase production by aspergillus oryzae in liquid media. | the alpha-amylase secretion in a mineral culture medium containing starch and glucose follow the lysis of mycelium. this lysis seems to result from the hydrolysing action of dextranase and levulanase on cell wall. cell lysis and amylase secretion are greatly enhanced by ph elevation of culture medium (optimal ph 8,8). in such conditions of production the amylase is not stable but can be stabilized by addition of starch. a method is described using ph and starch content modifications, which allow ... | 1977 | 23718 |
| isolation and properties of leucine aminopeptidase from aspergillus oryzae. | homogenious leucine aminopeptidase is purified from "oryzine"--mixture of enzymes produced by surface culture of asperigillus oryzae using treatment with activated characoal, followed by deae-cellulose and hydroxylapatite chromatographies, biogel p-100 gel-filtration and polyacrylamide-gel electrophoresis. the enzyme has ph optimum 9.0 and the molecular weight 37500 as estimated by gil-filtration through sephadex g-100 (superfine) and sds-polyacrylamide gel electrophoresis. leucine aminopeptidas ... | 1977 | 19097 |
| preparation and properties of a new dnase from aspergillus oryzae. | a dnase present in commercial preparations of aspergillus oryzae alpha-amylase was purified 1550-fold in 25% yield by acetone precipitation and by chromatography on diethylaminoethyl- and carboxymethylcellulose. the enzyme was isolated free of contaminating rnases and dnases. the molecular weight of the enzyme determined by gel filtration on sephadex g-100 was 48 000, while a molecular weight of 58 000 was determined for the single band observed upon polyacrylamide gel electrophoresis in sodium ... | 1977 | 19042 |
| rapid induction of alpha-amylase by nongrowing mycelia of aspergillus oryzae. | a rapid induction system for synthesis of alpha-amylase by the funga aspergillus oryzae m-13 was established. the mycelia were prepared from 20-h cultures grown on a peptone-glycerol medium and starved for 5 h; maltose was the optimum inducer tested. during h 1 of induction, formation of both intra- and extracellular alpha-amylases occurred at an almost identical rate (70 to 80 microgram/g of cells-h) without a detectable lag period. after a 1-h induction period, a remarkable increase in the ext ... | 1977 | 18989 |
| properties of chymotrypsin proteinase from aspergillus oryzae. | chymotrypsin-type proteinase is detected in the proteolytic system of asp. oryzae. the action of it and chymotrypsin is shown to depend on formaldehyde. hydrolysis of substrates, p-nitrophenyl acetate (p-npa) and n-benzoyl-tyrosine methyl ether (btme), by both preparations is almost the same. the obtained activity ph-optimum for the studied proteinase esterolytic activity is located in the alkaline zone as well as for crystalline chymotrypsin (substrate p-npa). it concerns ph of both enzymes sta ... | 1977 | 18830 |
| gluconic acid forming enzymes in aspergillus niger (author's transl). | at least three gluconic acid forming enzymes were identified in cell-free extracts of aspergillus niger: glucose oxidase (ec 1.1.3.4), a glucose dehydrogenase (ec 1.1.99.10), and an enzyme or a mixture of enzymes catalyzing the cleavage of 6-phosphogluconate into gluconate and inorganic phosphate. 2,6-dichlorphenolindophenol was one of the hydrogen acceptors in vitro of the glucose dehydrogenase. some properties of this enzyme (km values, ph-dependence, substrate and hydrogen acceptor specificit ... | 1977 | 16416 |
| extracellular acid protease of aspergillus oryzae grown on liquid media: multiple forms due to association with heterogeneous polysaccharides. | the acid protease (ec 2.4.23.6) that is produced extracellularly when aspergillus oryzae is grown on liquid media has been isolated and characterized. the enzyme was purified by precipitation with tannic acid, chromatography on duolite a-2, and gel filtration on sephadex g-100. the last step yielded four active components, with varying molecular weights ranging from 42,000 to 60,000. two of them, designated e1 and e1a, with molecular weights of 60,000 and 55,000, respectively, were heterogeneous ... | 1977 | 15987 |
| mechanism of conjugation and recombination in bacteria. xvii. further evidence for single-stranded regions in recipient dna during conjugation in escherichia coli k-12. | the secondary structure of recipient dna mated with hfr strain was investigated by cscl density gradient fractionation. after 45 min of hfrh64 x 3h-f-ab1157 mating one-fourth of the radioactive recipient dna was recovered as a single-strand but only after shearing of cell lysates prior to centrifugation. this heavier than native dna fraction of radioactive material (obtained after the first centrifugation) was degraded by single-strand specific nuclease s from aspergillus oryzae. these findings ... | 1977 | 67752 |
| evidence for the presence of membrane-bound forms of acid protease in aspergillus oryzae. | 1977 | 13792 | |
| isolation of amylolytic system of aspergillus oryzae by sorption on deahp amylum. | conditions of effective sorption of amylolytic enzyme from a solution or from fermentative liquid on deahp amylum were studied. isolating action is in a direct dependence on the relation between activity and amount of deahp amylum, the curve of this dependence was illustrated. the enzyme can be released by elution or adsorbate can be used in a pulverised from. in the conclusion of the work laboratory isolation technique is described. | 1977 | 15220 |
| use of amylum derivatives for isolation of amylolytic enzymes. | in a leading article literature is reviewed concerning isolation of amylolytic enzymes by adsorption on differently modified starches, resp. on other adsorbents. deae amylum, deahp amylum and deae-sephadex a 25 were found to be most suitable adsorbents. the other adsorbents examined did not reach claimed parameters. | 1977 | 15219 |
| release of escherichia coli dna from membrane complexes by single-strand endonucleases. | treatment of gently prepared lysates of escherichia coli with single-strand-specific endonuclease (si or from mung beans) results in the release of about 90% of the dna from membranes, as determined by the m band technique. the released dna has an average molecular weight of about 1.2 x 10(8). data obtained with endonuclease s1 fit a mathematical model in which substrate sites are at or near membrane attachment sites. data obtained with pancreatic deoxyribonuclease or x-rays fit a model for doub ... | 1977 | 331316 |
| [drug treatment of meteorism. results of a double blind test]. | 1977 | 333264 | |
| studies on the restoration of the activities of ribonucleases by polyamines in the presence of various ribonuclease inhibitors. | the effect of polyamines on ribonucleases in the presence of various inhibitors (poly(g), heparin, and rat liver rnase inhibitor) has been studied. bovine pancreatic rnas a and a ribonuclease from horse submaxillary gland (rnase hs) were inhibited by the inhibitors, but rnase t1 and rnase m were not inhibited. polyamines were found to restore the activites of rnase a and rnase hs inhibited by poly(g) or heparin but not those activities inhibited by rat liver rnase inhibitor. when poly(u) and pol ... | 1977 | 405377 |
| simple and rapid colorimetric method for the microdetermination of alpha amylase. | 1977 | 412348 | |
| characterization of dna used to assay sera for anti-dna antibodies; determination of the specificities of anti-dna antibodies in sle and non-sle rheumatic disease states. | commercial 14c-labeled kb cell dna, widely used to assay sera for anti-dna antibodies, was chromatographed on benzoylated-naphthoylated-deae-cellulose (bndc) and on hydroxyapatite (hap). on bndc, only 25% of the 14c label eluted with 1 m nac1 (kb fraction i) characteristic of ds-dna. fifty-five percent of the label eluted with 50% formamide-1 m nac1 (kb fraction ii) characteristic of ss or denatured dna. on hap, however, none of the 14c label eluted with 0.2 m phosphate buffer as anticipated for ... | 1977 | 300090 |
| studies on the respiratory system of aspergillus oryzae. v. some properties of the respiratory system of mitochondria from mycelia grown in the presence of chloramphenicol. | presence of chloramphenicol in the growth medium for mycelia of aspergillus oryzae was without effect on the oxidative activity, respiratory control, or p/o ratio of isolated mitochondria. the mitochondria oxidized krebs cycle intermediates even in the presence of cyanide at the concentration markedly inhibiting the normal mitochondrial oxidation. however, the p/o ratio during the mitochondrial oxidation decreased by about 1.0 on addition of cyanide. the c-type cytochromes, shown to occur in lar ... | 1977 | 199770 |
| inside 45s ribonucleic acid. | 1977 | 202281 | |
| aspergillus oryzae (nrrl strain 1988) | 1977 | 867035 | |
| kinetic studies of the phenol sulphate-phenol sulphotransferase of aspergillus oryzae. | arylsulphatase ii of aspergillus oryzae exhibits both hydrolytic and sulphotransferase activities. the kinetic data suggest the formation of an intermediate covalent enzyme-sulphate complex with transfer of sulphate from donor to acceptor proceeding via a ping pong mechanism. the unusual kinetic behaviour when 2-hydroxy-5-nitrophenyl sulphate is the substrate is also consistent with this mechanism. | 1977 | 588253 |
| release of peptide-bound sialic acid from landschütz ascites-tumour cells by proteinase 1 of aspergillus oryzae [proceedings]. | 1977 | 598591 | |
| mycotoxins of aspergillus oryzae strains for use in the food industry as starters and enzyme producing molds. | 1977 | 613924 | |
| mechanism of amylase action on glucoside starch bonds. | functional groups of glucoamylase and alpha-amylase from asp. awamori, alpha-amylase from asp. oryzae and alpha- and beta-amylases from barley malt are identified. kinetic curves of the activity dependency on ph, values of ionization heats and photooxidative inactivation draw to the conclusion that carboxyl-imidazole system enters into the active site of the enzymes. a hypothetic mechanism of hydrolysis of alpha-1,4-glucoside bond in starch molecule by alpha- and beta-amylases and of alpha-1,4- ... | 1976 | 14726 |
| characterization of beta-galactosidase from a special strain of aspergillus oryzae. | beta-galactosidase ec 3.2.1.23 was isolated from a partially purified preparation obtained from cultured cells of a special strain of aspergillus oryzae, rt 102 (ferm-p1680). the enzyme preparation gave a single protein band on polyacrylamide gel electrophoresis and was free from alpha-galactosidase, alpha- and beta-mannosidase, alpha- and beta-n-acetylhexosaminidase, and protease activities. the beta-galactosidase was capable of acting on aryl beta-galactosides, lactose, and lactosides. it also ... | 1976 | 14118 |
| purification and characterization of the two molecular forms of aspergillus oryzae acid protease. | the isolation and partial characterization of the acid proteases a1 and a2 (ec3.4.23.6) from aspergillus oryzae grown on solid bran culture are described. the purified preparations were essentially homogeneous by several criteria including sedimentation analysis and polyacrylamide gel electrophoresis. the physiochemical properties of the proteases a1 and a2 were as follows (in the order: a1, a2): molecular weight: 63 000 & 32 000; sedimentation coefficient s20, w: 3.93 and 3.16 s; diffusion cons ... | 1976 | 8138 |
| enzymatic determination of nonrandom incorporation of 5-bromodeoxyuridine in rat dna. | secondary cultures of normal rat embryo cells were synchronized by a double thymidine block and pulsed with 10(-7) m 5-[3h]bromodeoxyuridine (brdurd) or 10(-7) m[3h]thymidine during an entire s phase (7.5 h). to examine the pattern of [3h]thymidine, dna was immediately extracted and purified at the completion of the s phase, cscl density gradient centrifugation revealed that substitution for thymine by bromouracil was less than 7%. single-strand specific nucleases obtained from aspergillus oryza ... | 1976 | 133713 |
| [isolation and properties of the acid carboxypeptidase of aspergillus oryzae]. | caboxypeptidase with ph optimum 4--5 for peptide substrates is isolated from "oryzine", the enzyme mixture produced by aspergillus oryzae, by means of successive salt fractionation, sephadex g-75 gel filtration, chromatography on amberlite irc-50, hydroxylapatite, deae-cellulose and polyacrylamide gel electrophoresis. its molecular weight, as determined by means of polyacrylamide gel electrophoresis in the presence of sodium dolecylsulphate, was found to be 37 000. the enzyme has a broad substra ... | 1976 | 942547 |
| aspergillus oryzae (nrrl strain 1988): a clarification. | 1976 | 996551 | |
| renaturation of bacteriophage lambda dna. determination of the optimal renaturation conditions using a single-strand-specific dnase and alkaline-sucrose-gradient assay system. | reannealed hybrid molecules of wild-type bacteriophage lambda dna were prepared in aqueous solutions of formamide at a variety of nacl concentrations at both room temperature ( 22 degrees c) and 37 degrees c. treatment of the hybrid dna molecules with the single-strand-specific nuclease s1 from aspergillus oryzae followed by alkaline sucrose gradient sedimentation was used to monitor the extent and fidelity of hybridization. the optimal renaturation conditions at room temperature were found to b ... | 1976 | 1248479 |
| [hydrolysis of insoluble bone collagen by crystalline alpha-amylase from aspergillus oryzae]. | the paper deals with hydrolysis of bone collagen thrice recrystallized alpha-amylase. the enzyme action was estimated by the amount of released glycopeptides, free carbohydrates, alpha-amine groups and total nitrogen in the soluble part of the hydrolyzate. the protease admixture in the alpha-amylase preparation was found by means of the aspergillus oryzae protease. the data obtained testify to the fact that hydrolysis of collagen under the effect of crystalline alpha-amylase occurs only due to t ... | 1976 | 1021920 |
| structure of cerebroside in aspergillus oryzae. | structural studies on cerebroside isolated from aspergillus oryzae were carried out using mainly gas-liquid chromatography-mass spectrometry. the major component fatty acids were 2-hydroxystearic and 2-hydroxy-trans-octadecenoic acid; branched 17?-methyl nonadecasphingadienine isomers were the predominant long-chain bases. the component sugar was only glucose. the structure of the major cerebrosides was assumed to be n-2'-hydroxystearoyl-1-o-glucosyl-17?-methyl sphingadienine and n-2'-hydroxyoct ... | 1976 | 1009131 |
| proteinase inhibitors from cereal grains. | 1976 | 1012027 | |
| effect of brinase (protease i from aspergillus oryzae) on the elimination of fibrin from the lungs and pulmonary damage in rats with induced intravascular coagulation and inhibited fibrinolysis. | 1976 | 1006629 | |
| aspergillus oryzae meningitis. | in a patient with only meningitis, a septate hypha was seen in a langhans giant cell, and the rarely pathogenic aspergillus oryzae was cultured from the cerebrospinal fluid. serologic results confirmed the diagnosis. the patient responded to therapy with amphotericin b and flucytosine. | 1976 | 946540 |
| [degradation of the human igg molecule by a fungal fraction with leucineaminopeptidase activity. preliminary results]. | 1976 | 819165 | |
| studies on lipids of natto. | 1976 | 815306 | |
| [peptidases of preparations of proteolytic enzymes from several species of bacteria, molds and actinomycetes]. | the activity of carboxy- and aminopeptidases of enzymic preparations obtained from bacteria bacillus subtilis 1 m, 17, bacillus mesentericus 100/26, 100/56, 16; pseudomonas aeruginosa 1/7a, 1/4; bacillus thermophilicus s. sp. nov b-22, moulds aspergillus oryzae 1-746, aspergillus flavus 716 and actinomycete streptomyces griseus 32-13 was studied. in the bacterial preparations the proteolytic activity was 1.5-2.5 times lower than in the fungal and actinomycete preparations. the carboxypeptidase a ... | 1976 | 825850 |
| fungus-fermented soybeans benefit the life cycle of japanese quail (coturnix coturnix japonica). | feeding quail chicks diets containing soybeans fermented with two cultures of aspergilli (a. oryzae n.r.r.l. 451 and a. oryzae n.r.r.l. 506) resulted in significantly superior weight gains (p less than 0.05) through a 4-week growth period and confirmed previous observations made with identical cultures in broiler studies. subsequent hen-day egg production and egg size were changed little by diets containing fermented soybeans. the numerical increases in fertility and hatchability were not signif ... | 1976 | 945574 |
| quantitative analysis of the action of taka-amylase a on maltotriose. | the action of taka-amylase a from asp. oryzae was studied quantitatively by the product analysis method using unlabeled maltotriose and maltotriose labeled at the reducing end as substrates. it was found that the ratio of the unlabeled products, maltose (g2) and glucose (g1) exceeded unity, and that the ratio of the labeled products, g2/g1 was strongly dependent on the initial substrate concentrations. the results can only be explained by a transglycosylation or condensation mechanism or both. a ... | 1976 | 977557 |
| [production of immobilized alpha-amylase and its properties]. | active immobilized alpha-amylase was obtained when applying ae-cellulose chromatography and glutaric dialdehyde. the time of the enzyme interaction with the carrier, amount of glutaric dialdehyde necessary for binding, optimal enzyme: carrier ratio as well as the methods for desiccation of the immobilized amylase preparations were specified. conditions are selected for alpha-amylase stabilization with the presence of glutaric aldehyde. immobilized amylase as compared to free enzyme is shown to b ... | 1976 | 982618 |
| fermentative production of limit dextrinase by aspergillus oryzae. | 1976 | 992794 | |
| attachment of thioglycosides to proteins: enhancement of liver membrane binding. | thioglycosides of d-galactose, d-glucose, n-acetyl-d-glucosamine, and d-mannose were covalently attached to aspergillus oryzae alpha-amylase, hen's eggs lysozyme, and bovine serum albumin by amidination, diazo coupling, and amide formation. the binding of the newly formed glycoproteins (neoglycoproteins) to rabbit liver membranes was measured, using asialoorosomucoid as a reference. attachment of d-galactosides by any of the three methods enhanced binding by several orders of magnitude. coupling ... | 1976 | 963013 |
| [effect of the storage conditions on the viability of fungi]. | 1976 | 950926 | |
| subsite mapping of enzymes. application of the depolymerase computer model to two alpha-amylases. | in the preceding paper (allen and thoma, 1976) we developed a depolymerase computer model, which uses a minimization routine to establish a subsite map for a depolymerase. in the present paper we show how the model is applied to experimental data for two alpha-amylases. michaelis parameters and bond-cleavage frequencies for substrates of chain lengths up to twelve glucosyl units have been reported for bacillus amyloliquefaciens, and a subsite map has been proposed for this enzyme [thoma et al. ( ... | 1976 | 999630 |
| conformation of mammalian 5.8 s ribosomal rna: s1 nuclease as a probe. | 1976 | 1001452 | |
| the structure of kinetoplast dna. 2. characterization of a novel component of high complexity present in the kinetoplast dna network of crithidia luciliae. | 1. degradation of highly purified kinetoplast dna (kdna) networks with restriction endonucleases yields "extra" bands in agarose gels that are absent from digests of mini-circles. each of the five endonucleases tested, i.e. alui, hapii, ecori, hsu and hindii + iii, yields a unique set of "extra" bands. the "extra" bands consist of linear dna; they are not mini-circle oligomers and their added molecular weight, calculated from mobility in gels, are around 2 x 10(7). double digests with two restri ... | 1976 | 1278151 |
| aflatoxin production by a variant of aspergillus oryzae (nrrl strain 1988) on cowpeas (vigna sinensis). | aflatoxin b1, b2, g1, and g2 are produced when a variant of aspergillus oryzae (nrrl strain 1988) is grown on cowpeas or rice. the present study indicates that a strain of aspergillus oryzae approved for use in food processing is variable and the resulting variant, unlike the parent strain, has a propensity to produce aflatoxin. | 1976 | 1273594 |
| the in situ formation of dna-dna duplexes of drosophila virilis satellite dna. | the dna of drosophila virilis brains and imaginal discs was labeled in vitro to a specific activity of 6 x 10(-5) dpm/mug, using an organ culture medium. the dna was fractionated on neutral and alkaline csc1 gradients and the heavy strands of satellite i annealed in situ to denatured polytene chromosomes from squash preparations of larval salivary glands. nuclease s1 from aspergillus oryzae was used to digest the unpaired ssdna, resulting in a distinct labeling of the alpha-heterochromatin in th ... | 1976 | 1253642 |
| nuclease s1 cleavage and the primary structure of mitochondrial dna. | the single-strand-specific nuclease s1 from aspergillus oryzae rapidly converts superhelical mitochondrial dna (african green monkey cells, vero atcc; ccl 81) into nicked circular dna. these nicked mitochondrial dna molecules contain two nicks, one in each strand. the phosphodiester backbones are cleaved during this reaction at or near sites that are alkali-labile. in a second slow reaction the circular mitochondrial dna is converted into a linear duplex dna. permutation tests indicate that this ... | 1976 | 1261542 |
| purification and properties of the nuclease inhibitor of aspergillus oryzae and kinetics of its interaction with crystalline nuclease o. | a nuclease inhibitor found in the mycelia of aspergillus oryzae has been purified 158,000-fold by ammonium sulfate precipitation, chromatography on sephadex g-75, deae-sephadex a-50 and bio-gel p-60 columns, preparative disc electrophoresis on acrylamide gel, and electrofocusing in ampholite. the purified inhibitor is nearly homogeneous as judged by disc electrophoresis. it shows a typical ultraviolet absorption curve for protein, and the inhibitory activity is inactivated by chymotrypsin. the i ... | 1976 | 1262346 |
| sepcific fragmentation of dna heteroduplex molecules of two bacteriophage lambda mutants with endonuclease si from aspergillus oryzae. | heteroduplex dna molecules of two bacteriophage mutants (lambda b2 and lambda i434ct68) were obtained by the method of molecular hybridization. these heteroduplexes possessed two types of loops formed as a result of: a) deletion in one of the dna strands; and b) substitution of a dna fragment for nonhomological one. the digestion of heteroduplexes with single-stranded specific nuclease si from aspergillus oryzae produced two fragments at 37 degrees c and three ones at 55 degrees c. the separatio ... | 1976 | 1272803 |
| visualization of leucineaminopeptidase activity after acrylamide gel electrophoresis. | 1976 | 962113 | |
| protein and amino acid changes in peanut (arachis hypogaea l.) seeds infected with aspergillus oryzae. | 1976 | 1245677 | |
| reversal of fungitoxicity of 8-quinolinols and their copper (ii) bischelates. i. amino acids and related compounds. | the effect of amino acids and related compounds on the toxicity of 8-quinolinols and their copper (ii) bischelates to aspergillus oryzae (atcc 1011) was studied. none of the compounds tested except the thiol-containing compounds, cysteine, cysteamine, glutathione, and n-acetylcysteine reversed the inhibitory action of 8-quinolinol but not that of 5-iodo-8-quinolinol or bis (8-quinolinolato) copper (ii). it appears that the mechanism(s) of fungitoxicity of 8-quinolinol is different from that of 5 ... | 1975 | 1116048 |
| specific hydrolysis of the cohesive ends of bacteriophage lambda dna by three single strand-specific nucleases. | procedures have been worked out for aspergillus nuclease s1 and mung been nuclease to quantitatively cleave off both of the 12-nucleotide long, single-stranded cohesive ends of lambdadna. this cleavage is indicated by the almost complete elimination of the repair incorporation of radioactive nucleotides by dna polymerase into the digested dna. with s1 nuclease, cleavage was complete at 10 degrees as well as at 30 degrees. under the conditions for quantitative cleavage of the single-stranded regi ... | 1975 | 1141222 |
| [action of leucine aminopeptidase 1 and 2 (aspergillus oryzae 410) on various synthetic peptides (author's transl)]. | by use of di- or tripeptides as substrates, lap 1 and lap 2, 2 fractions from aspergillus oryzae hydrolyze oligopeptides that possess leucine as n-terminal amino acid. lap 1 fraction also hydrolyzes the histidyl bond. both fractions have no activity towards peptides as glutathion, gly-pro-ala; they have low or no activity towards tyrosyl and tryptophanyl bond. | 1975 | 1157844 |
| triacetonamine formation in fungal extracts. | 1975 | 1159172 | |
| in vitro recognition of carcinogen-induced local denaturation sites native dna by s1 endonuclease from aspergillus oryzae. | 1975 | 1161018 | |
| purification and some properties of the soluble and membrane-bound adenosine deaminases of micrococcus sodonensis atcc 11880 and their distribution within the family micrococcacea. | in micrococcus sodonensis and some other micrococcus species, adenosien deaminase is present both as a membran-bound and a soluble enzyme; the membran-bound adenosine deaminase can be extracted with n-butanol, and may account for up to 5% of the total cellular adenosine deaminase activity. in a number oc comparative tests, no differences between the two enzyme forms could be found, thus they are believed to be similar molecular species; the purified membran-bound or soluble enzyme had a molecula ... | 1975 | 1168529 |
| a chemical and biological assessment of aspergillus oryzae and other filamentous fungi as protein sources for simple stomached animals. | 1975 | 1172167 |