Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| factors affecting human supragingival biofilm composition. i. plaque mass. | little is known about the factors that affect the microbial composition of supragingival biofilms. this study was designed to examine the relationship between total dna probe counts of supragingival biofilm samples, clinical parameters and supragingival biofilm composition. | 2009 | 18973540 |
| crystal structure of fada adhesin from fusobacterium nucleatum reveals a novel oligomerization motif, the leucine chain. | many bacterial appendages have filamentous structures, often composed of repeating monomers assembled in a head-to-tail manner. the mechanisms of such linkages vary. we report here a novel protein oligomerization motif identified in the fada adhesin from the gram-negative bacterium fusobacterium nucleatum. the 2.0 angstroms crystal structure of the secreted form of fada (mfada) reveals two antiparallel alpha-helices connected by an intervening 8-residue hairpin loop. leucine-leucine contacts pla ... | 2009 | 18996848 |
| the fusobacterium nucleatum outer membrane protein radd is an arginine-inhibitable adhesin required for inter-species adherence and the structured architecture of multispecies biofilm. | a defining characteristic of the suspected periodontal pathogen fusobacterium nucleatum is its ability to adhere to a plethora of oral bacteria. this distinguishing feature is suggested to play an important role in oral biofilm formation and pathogenesis, with fusobacteria proposed to serve as central 'bridging organisms' in the architecture of the oral biofilm bringing together species which would not interact otherwise. previous studies indicate that these bacterial interactions are mediated b ... | 2009 | 19007407 |
| characterization of coaggregation of fusobacterium nucleatum pk1594 with six porphyromonas gingivalis strains. | coaggregation is a key mechanism in biofilm formation. the aim of this study was to characterize the coaggregation of fusobacterium nucleatum pk1594 with six porphyromonas gingivalis strains in terms of kinetics and sugars inhibition. this coaggregation was quantitatively characterized by using a kinetic coaggregation assay. sugar inhibition profiles were also quantitatively defined. four types of interactions among these coaggregation partners were found: (1) fast coaggregation that was substan ... | 2009 | 19084124 |
| enhanced attachment of porphyromonas gingivalis to human fibroblasts mediated by fusobacterium nucleatum. | fusobacterium nucleatum and porphyromonas gingivalis are often co-isolated from endodontic infection sites. the present study tested the hypothesis that adhesin-mediated reactions might contribute to this phenomenon. f. nucleaum pk1594 and p. gingivalis pk1924 were selectively labeled with fluorochromes and allowed to adhere to human fibroblasts, either each strain alone or sequentially. the number of bacteria of each type adhering to individual fibroblasts was determined. sugar inhibition profi ... | 2009 | 19084131 |
| synergistic pathogenicity of porphyromonas gingivalis and fusobacterium nucleatum in the mouse subcutaneous chamber model. | porphyromonas gingivalis and fusobacterium nucleatum are often coisolated from sites of infection, such as suppurative apical periodontitis. the synergistic pathogenicity of mixed infection of p. gingivalis hg 405 with f. nucleatum pk 1594 was studied in the mouse subcutaneous chamber model in groups of seven animals. the minimal dose for p. gingivalis hg 405 that was required to infect 100% of the chambers was reduced by 1,000-fold when animals were inoculated in the same chamber with 1 x 10(9) ... | 2009 | 19084132 |
| oral bacteria modulate invasion and induction of apoptosis in hep-2 cells by pseudomonas aeruginosa. | pseudomonas aeruginosa is an important opportunistic bacterial pathogen, causing infections of the respiratory and other organ systems in susceptible hosts. p. aeruginosa infection is initiated by adhesion to and invasion of mucosal epithelial cells. the failure of host defenses to eliminate p. aeruginosa from mucosal surfaces results in p. aeruginosa proliferation, sometimes followed by overt infection and tissue destruction. there is growing evidence that associates poor oral health and respir ... | 2009 | 19041936 |
| tinidazole inhibitory and cidal activity against anaerobic periodontal pathogens. | the in vitro activity of tinidazole against anaerobic periodontal pathogens (25 prevotella buccae, 18 prevotella denticola, 10 prevotella intermedia, 6 prevotella melaninogenica, 5 prevotella oralis, 10 fusobacterium nucleatum and 8 veillonella spp.) was determined by agar dilution. mic(90) values (minimum inhibitory concentration for 90% of the organisms) were 8 microg/ml for veillonella spp., 4 microg/ml for p. intermedia, 2 microg/ml for p. buccae, 1 microg/ml for fusobacterium spp. and 0.5 m ... | 2009 | 19097755 |
| periodontal pathogen levels in adolescents before, during, and after fixed orthodontic appliance therapy. | this purpose of this study was to document and investigate changes in periodontal pathogen levels before, during, and after orthodontic treatment in adolescents. | 2009 | 19121507 |
| short-term clinical and microbiological evaluations of peri-implant diseases before and after mechanical anti-infective therapies. | the aim of this study was to evaluate the clinical and microbiological effects of mechanical anti-infective therapies for mucositis and peri-implantitis. | 2009 | 19126114 |
| the microbiota of acute apical abscesses. | as the breadth of bacterial diversity in the oral cavity has been deciphered by molecular studies, several newly identified species/phylotypes have emerged as potential pathogens. we hypothesized that many of these species/phylotypes could also be involved with the etiology of endodontic abscesses. abscess aspirates from 42 persons were analyzed for the presence of 81 species/phylotypes by means of a reverse-capture checkerboard hybridization assay. associations between the most frequently detec ... | 2009 | 19131319 |
| construction of an ileal reservoir in patients with a previous straight endorectal ileal pull-through. | a decade has passed since martin and associates presented the first series of endorectal ileal pull-through (pt) procedures with straight ileoanal anastomosis for treatment of ulcerative colitis and polyposis. during the ensuing years, several medical centers have reported their results using the pt. although some children have experienced good results, the majority of adults and children have had severe problems associated with high stool frequency, urgency, and incontinence. between 1977 and 1 ... | 2009 | 2839124 |
| construction of an ileal reservoir in patients with a previous straight endorectal ileal pull-through. | a decade has passed since martin and associates presented the first series of endorectal ileal pull-through (pt) procedures with straight ileoanal anastomosis for treatment of ulcerative colitis and polyposis. during the ensuing years, several medical centers have reported their results using the pt. although some children have experienced good results, the majority of adults and children have had severe problems associated with high stool frequency, urgency, and incontinence. between 1977 and 1 ... | 2009 | 2839124 |
| hope for aids vaccines. | 2009 | 2781299 | |
| [plastic surgery in otorhinolaryngology: reconstruction flaps in oncologic surgery]. | 2009 | 2701493 | |
| effects of early maturation on fetal growth. | while the association of young maternal age with low birthweight (lbw) is well known, the contribution of early menarche to this association has never been studied. we examined the effects of early menarche (less than or equal to 11 years) on lbw and its two major underlying causes: pre-term delivery and smallness-for-gestational-age (sga). results represent a narrow chronological age range, 17-18 years, of a larger geographically based cohort of 2789 pregnant adolescents. adjusted odds ratios ( ... | 2009 | 2782851 |
| allocation and the physician: the impacts of aging and aids. | 2009 | 2709175 | |
| 31p-nmr study on intracellular energy transport in muscle. | 2009 | 2798486 | |
| 31p-nmr study on intracellular energy transport in muscle. | 2009 | 2798486 | |
| transcardiac passage of thrombus--ultrasonographic visualization. | a patient is described in whom a right atrial thrombus was seen, by two-dimensional echocardiography, in the act of embolizing into the pulmonary circulation. | 2009 | 2702500 |
| [essential arterial hypertension in patients with non-insulin-dependent diabetes mellitus during and after balneotherapy followed-up for 6 years]. | the statistical correlations between body weight, glycemia, total lipids, cholesterol in 200 patients with essential hypertension, especially borderline essential hypertension, followed up for 6 years indicate that the complex environmental factors in slănic-moldova health resort associated with balneotherapy may induce a return to normal values of the above mentioned parameters. good results were also obtained in the patients with essential hypertension and non-insulin dependent diabetes mellit ... | 2009 | 2636767 |
| an unusual autopsy case of pyogenic liver abscess caused by periodontal bacteria. | pyogenic liver abscess (pla) formation is thought to originate from the transmission of infection via three major routes including the biliary tract, portal vein and hepatic artery. however, about 50% of pla cases are considered to be cryptogenic. here we report an unusual autopsy case of pla associated with periodontopathic bacterial infection. a 59-year-old female suddenly developed cardiopulmonary arrest and died. despite macroscopic and microscopic examinations, the infectious routes and sou ... | 2009 | 19762989 |
| kinetic characterization and quaternary structure of glutamate racemase from the periodontal anaerobe fusobacterium nucleatum. | cofactor-independent glutamate racemases (grs) that supply the d-glutamate required for biosynthesis of the peptidoglycan that encapsulates bacterial cells are attractive targets for the development of antibacterial drugs. recombinant gr from fusobacterium nucleatum (fngr), a gram-negative anaerobe involved in periodontal disease, was overproduced, purified, and characterized. unlike most other grs, fngr is a pseudosymmetric enzyme, catalyzing the racemization of glutamate enantiomers with simil ... | 2009 | 19772853 |
| differential ability of periodontopathic bacteria to modulate invasion of human gingival epithelial cells by porphyromonas gingivalis. | periodontitis is a polymicrobial infection caused by selected gram-negative bacteria including porphyromonas gingivalis. host cell invasion by p. gingivalis has been proposed as a possible mechanism of pathogenesis in periodontitis. the aim of the present study was to assess the influence of periodontopathogens on p. gingivalis invasion of gingival epithelial cells in polymicrobial infection. p. gingivalis was tested for its ability to invade a human gingival epithelial cell line ca9-22 in co-in ... | 2009 | 19818393 |
| fusobacterium bacteremia: clinical significance and outcomes. | fusobacterium bacteremia is uncommon, accounting for approximately 0.9% of patients with bacteremia. the objectives of this study were to evaluate the incidence and clinical significance of blood cultures positive for fusobacterium spp., risk factors for mortality, and the impact of antimicrobial therapy on clinical outcomes. | 2009 | 19949758 |
| toll-like receptor 2 and nalp2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells. | we previously reported that infection by fusobacterium nucleatum strongly induced the expression of both human beta-defensin 2 (hbd-2) and hbd-3 by gingival epithelial cells. the aim of this study was to characterize the pattern recognition receptors (prrs) and regulatory mechanisms involved in the induction of hbd-2 and -3 expression by f. nucleatum in gingival epithelial cells. immortalized human gingival epithelial hok-16b cells were infected with live or heat-killed f. nucleatum, and the exp ... | 2009 | 19103770 |
| transmission of periodontopathic bacteria from natural teeth to implants. | purpose: prevention of peri-implantitis is essential for the success of implant rehabilitation. infection by periodontopathic bacteria is a major cause of peri-implantitis. the aim of the present study was to identify the source of peri-implant colonization by periodontopathic bacteria. materials and methods: twenty-one patients with implants were enrolled in the study. subgingival plaque samples from the adjacent, occluding, and contralateral natural teeth were collected prior to second-stage s ... | 2009 | 20002682 |
| phenotypic and genotypic analyses of clinical fusobacterium nucleatum and fusobacterium periodonticum isolates from the human gut. | fusobacterium nucleatum is a gram-negative anaerobic rod that is part of the normal human microflora, and has also been associated with various infections. bacterial strains belonging to the species are typically heterogeneous in both phenotype and genotype, which can hinder their identification in a clinical setting. the majority of f. nucleatum isolates originate from oral sites, however the species is also a resident of the human gastrointestinal tract. the aim of this study was to compare f. ... | 2008 | 19114111 |
| tongue biofilm areal density and tongue coating index. | two methods have been employed to measure the amount of tongue biofilm in humans: (1) areal density of microbes (cfu cm(-2)) (tongue scrape from a known/measured area), or (2) a tongue-coating index, where a human judge assesses the amount of 'coating' by visual inspection. the two methods were compared, both on human volunteers and on artificial biofilms. fusobacterium nucleatum (fn), prevotella intermedia (pi), streptococcus mutans (sm) and peptostreptococcus micros (pm) were obtained from sto ... | 2008 | 21386152 |
| generation of indole/skatole during malodor formation in the salivary sediment model system and initial examination of the oral bacteria involved. | gram negative anaerobic microbial degradation of proteins, peptides and amino acids in saliva leads to production of oral malodor. volatile sulfur compounds (vscs) from cysteine and indole/skatole (i/s) from tryptophan are two major components of breath odor. in this study, salivary mixed bacteria and oral pure cultures were compared for their ability to produce odor from cysteine, tryptophan and salivary supernatant. the vsc malodor inhibitor, zinc, was used to help identify the malodor process ... | 2008 | 21386161 |
| [measurement of orthodontic appliance force using prototype sensors]. | in introductory remarks the most important biomechanical principles, which are relevant for the research, and conditions which must be satisfied presented in intraoral measuring of orthodontic forces were pointed out. first the aim of the research was defined, and then the investigation of mechanical properties of acrylics as a material for orthodontic appliances were carried out. the type of strain gauges, bonds and measuring system configurations, as well as measuring amplifiers and recording ... | 2008 | 2635572 |
| two cytochrome p-450 isoforms catalysing o-de-ethylation of ethoxycoumarin and ethoxyresorufin in higher plants. | the o-dealkylating activities of 7-ethoxycoumarin o-de-ethylase (ecod) and 7-ethoxyresorufin o-de-ethylase (erod) have been fluorimetrically detected in microsomes prepared from manganese-induced jerusalem artichoke tubers. cytochrome p-450 dependence of the reactions was demonstrated by light-reversed co inhibition, nadph-dependence, nadh-nadph synergism and by use of specific inhibitors: antibodies to nadph-cytochrome p-450 reductase, mechanism-based inactivators and tetcyclasis. apparent km v ... | 2008 | 2241905 |
| [changes in lymphocytes in acute suppurative-destructive lung diseases]. | a study of the content of lymphocytes and their populations, indices of intracellular viral involvement of the lymphocytes in 32 patients with acute purulent-destructive pulmonary diseases and 46 healthy persons revealed an increase of the percent of non-viable lymphocytes containing viral inclusions. viral involvement of lymphocytes is considered one the leading causes of increased lymphocyte death. | 2008 | 2588523 |
| [flumazenil]. | 2008 | 2493168 | |
| differential expression of the il-2 receptor subunits, p55 and p75 on various populations of primary peripheral blood mononuclear cells. | unstimulated pbl were examined for expression of il-2r subunits, il-2rp55 and il-2rp75, by two-color flow cytometric analyses using mab. nkh-1+ non-t non-b cells expressed il-2rp75 but not il-2rp55, and the il-2rp75 sites on purified nkh-1+ cells were determined to be 1630 sites/cell by binding of 125i-labeled tu27 mab specific for il-2rp75. in the cd4+ t cell population, il-2rp55+ cells were significantly detected, but little or marginally of the il-2rp75+ cells. however, il-2rp75+ cells were s ... | 2008 | 2584707 |
| recovery from coma that results as a complication of cardiac arrest followed by cardiopulmonary bypass. | many possible complications are associated with cardiopulmonary bypass. they are similar to the risks accompanying most surgical procedures and include stroke, renal failure, and death. this potential for complication increases when bypass exceeds 2 hours and rises sharply when pump time is prolonged more than 3 to 4 hours. one group of serious complications is major neurologic disorders. the risk of significant cerebral dysfunction, that is, severe focal stroke or coma, is about 1%, and this ri ... | 2008 | 2584045 |
| [clinical study of osteoporosis]. | 2008 | 2614196 | |
| [bacteriological analysis of subgingival plaque in adolescents]. | to investigate the changes in characteristics of periodontal pathogens in subgingival plaque of patients with puberty gingivitis and its relevance with clinical symptoms. | 2008 | 19134350 |
| [detection of periodontal pathogenic bacteria dna in coronary atheromatous plaques from patients underwent coronary artery bypass graft]. | the aim of this study was to observe the coexistence of periodontal bacteria dna (actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, tannerella. forsythensis) in coronary atheromatous plaques and subgingival plaques in patients scheduled for coronary artery bypass graft. | 2008 | 19099976 |
| fusobacterium nucleatum enhances invasion of human gingival epithelial and aortic endothelial cells by porphyromonas gingivalis. | invasion by porphyromonas gingivalis has been proposed as a possible mechanism of pathogenesis in periodontal and cardiovascular diseases. porphyromonas gingivalis have direct access to the systemic circulation and endothelium in periodontitis patients by transient bacteremia. periodontitis can be described as one of the predominant polymicrobial infections of humans. in the present study, p. gingivalis strains were tested for their ability to invade a human gingival epithelial cell line (ca9-22 ... | 2008 | 19049647 |
| coaggregation of treponema denticola with porphyromonas gingivalis and fusobacterium nucleatum is mediated by the major outer sheath protein of treponema denticola. | coagreggation of treponema denticola with either porphyromonas gingivalis or fusobacterium nucleatum was characterized and the role of the major outer sheath protein (msp) in the coaggregation process of these bacteria was evaluated. the msp of t. denticola was found to be able to bind to p. gingivalis and f. nucleatum cells and this binding could be inhibited by msp in a concentration-dependent manner. while sodium dodecyl sulfate polyacrylamide gel electrophoresis and periodic acid-schiff (pas ... | 2008 | 19054094 |
| proteolytic activities of oral bacteria on prommp-9 and the effect of synthetic proteinase inhibitors. | tissue reactions to bacteria lead to proinflammatory reactions involving matrix metalloproteinases (mmps). synthetic protease inhibitors may offer new possibilities to regulate bacterial proteases. we investigated proteolytic activities of certain periodontal bacteria, their effects on the latent prommp-9, and the effects of synthetic mmp inhibitors and a serine protease inhibitor pefabloc. the strains studied were porphyromonas gingivalis, prevotella intermedia, peptostreptoccus micros, prevote ... | 2008 | 19088890 |
| frequency, microbial interactions, and antimicrobial susceptibility of fusobacterium nucleatum and fusobacterium necrophorum isolated from primary endodontic infections. | this study assessed the prevalence and microbial interactions of fusobacterium nucleatum and fusobacterium necrophorum in primary endodontic infections from a brazilian population and their antimicrobial susceptibility to some antibiotics by the e-test. one hundred ten samples from infected teeth with periapical pathologies were analyzed by culture methods. five hundred eighty individual strains were isolated; 81.4% were strict anaerobes. f. nucleatum was found in 38 root canals and was associat ... | 2008 | 19026872 |
| streptococcus cristatus attenuates fusobacterium nucleatum-induced interleukin-8 expression in oral epithelial cells. | oral epithelial cells may be invaded by a polymicrobial intracellular flora, including pathogens together with commensals. various oral pathogens can induce the production of interleukin-8, a potent neutrophil chemotractant, in oral epithelial cells. evidence from the gut suggests that commensal species may modulate inflammatory responses to pathogens. the aim of this study was to examine the interleukin-8 responses of oral epithelial cells to an oral pro-inflammatory species, fusobacterium nucl ... | 2008 | 18942189 |
| participation of glutathione in the elimination of porphyromonas gingivalis in vivo. | glutathione is involved in immune responses such as cell proliferation and bactericidal activity. the aim of this study was to see whether glutathione influences the intraperitoneal elimination of porphyromonas gingivalis in fusobacterium nucleatum-immunized mice. | 2008 | 18954348 |
| naturally occurring phenolic antibacterial compounds show effectiveness against oral bacteria by a quantitative structure-activity relationship study. | natural and synthetic phenolic compounds were evaluated against oral bacteria. a quantitative structure-active relationship approach was applied to the germ-kill activity for a range of phenolic compounds. the lipophilicity and steric effects were found to be two key factors in determining germ-kill activity. the optimum lipophilicity, measured by the logarithm of the octanol/water partition coefficient, or log p, was found to be 5.5 for fusobacterium nucleatum , a gram-negative type of oral bac ... | 2008 | 19007234 |
| comparison of culture methods and multiplex pcr for the detection of periodontopathogenic bacteria in biofilm associated with severe forms of periodontitis. | conventional culture methods and multiplex pcr, both of which we have been used for a long time in our clinical microbiology laboratory, were compared for their ability to detect a selected panel of periodontopathic bacteria: aggregatibacter actinomycetemcomitans, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, and prevotella intermedia. tests were performed in a single subgingival sample taken from a periodontal diseased site with a probing depth eq ... | 2008 | 18843894 |
| photodynamic therapy as an adjunct to non-surgical periodontal treatment: a randomized, controlled clinical trial. | recent preclinical and clinical data have suggested a potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of this study was to evaluate the clinical and microbiologic effects of the adjunctive use of pdt to non-surgical periodontal treatment. | 2008 | 18771363 |
| identification of a methioninase inhibitor, myrsinoic acid b, from myrsine seguinii lév., and its inhibitory activities. | a methioninase inhibitor from myrsine seguinii was purified and identified as myrsinoic acid b. its inhibitory activities as to crude methioninase from periodontal bacteria such as fusobacterium nucleatum, porphyromonas gingivalis, and treponema denticola were determined. the ic50 values were 10.5, 82.4, and 30.3 microm respectively. | 2008 | 18776663 |
| [characterization and antibacterial effect of ag-nha-ntio2/polyamide 66 nanocomposite membrane on oral bacteria]. | undried silver-hydroxyapatite-titania (ag-nha-ntio2) nanoparticles slurry was used to make membrane with polyamide 66 (pa66) by co-polymerization method. the purpose of this study is to test the physical and chemical characteristics and antibacterial ability. | 2008 | 18780488 |
| [bacteremia after periodontal procedures]. | bacteremia frequently occurs after oral surgery and odontology procedures. periodontitis may affect the incidence and bacterial spectrum of bacteremia. periodontal disease may be a significant risk factor for the development of certain systemic diseases. this study has aimed to evaluate the frequency of aerobic and anaerobic bacteria in the bloodstream following scaling and root planing. | 2008 | 18792815 |
| the role of toll-like receptors 2 and 4 on reactive oxygen species and nitric oxide production by macrophage cells stimulated with root canal pathogens. | periapical lesions arise as a result of the activation and interaction of the host immune responses against root canal infection. recently identified toll-like receptors (tlr) seem to be involved in the recognition and development of immune responses against a myriad of microorganisms. however, very little information is available on the role of tlr in the induction of periapical lesions. | 2008 | 18793356 |
| rapid identification of oral anaerobic bacteria cultivated from subgingival biofilm by maldi-tof-ms. | to facilitate the identification of anaerobes cultivated from periodontal disease, whole cell bacterial identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) was evaluated. | 2008 | 18793359 |
| molecular identification and quantification of bacteria from endodontic infections using real-time polymerase chain reaction. | it was the aim of the present study to evaluate root canal samples for the presence and numbers of specific species as well as for total bacterial load in teeth with chronic apical periodontitis using quantitative real-time polymerase chain reaction (pcr). | 2008 | 18793361 |
| stimulation of epithelial cell matrix metalloproteinase (mmp-2, -9, -13) and interleukin-8 secretion by fusobacteria. | bacterial pathogens involved in periodontal diseases exert their destructive effects primarily by stimulating the host cells to increase their secretion of proinflammatory cytokines and matrix metalloproteinases (mmps). this study aimed to determine the epithelial cell matrix metalloproteinase and interleukin-8 (il-8) secretion upon exposure to fusobacteria. | 2008 | 18793368 |
| fusobacterial infections: clinical spectrum and incidence of invasive disease. | clinically significant infections caused by members of the genus fusobacterium are rare. we sought to describe the spectrum of clinical disease and epidemiology of these conditions presenting to an acute hospital over a five year period. | 2008 | 18805588 |
| effects of low-energy shock waves on oral bacteria. | we have recently demonstrated that extracorporeal shock-wave therapy (eswt) is effective in promoting the healing of dermal wounds and in regenerating alveolar bone lost through periodontal disease. the objective of the present study was to determine any antibacterial effect of eswt on oral bacteria. monoculture suspensions of 6 bacterial species were treated with 100 to 500 pulses of eswt at energy flux densities (efd) of 0.12 mj/mm(2), 0.22 mj/mm(2), and 0.3 mj/mm(2). following treatment, aliq ... | 2008 | 18809745 |
| olfactory perception of cysteine-s-conjugates from fruits and vegetables. | volatile sulfur compounds have a low odor threshold, and their presence at microgram per kilogram levels in fruits and vegetables influences odor quality. sensory analysis demonstrates that naturally occurring, odorless cysteine- s-conjugates such as s-( r/ s)-3-(1-hexanol)- l-cysteine in wine, s-(1-propyl)- l-cysteine in onion, and s-(( r/ s)-2-heptyl)- l-cysteine in bell pepper are transformed into volatile thiols in the mouth by microflora. the time delay in smelling these volatile thiols was ... | 2008 | 18811169 |
| [multiple brain abscesses of odontogenic origin caused by fusobacterium nucleatum]. | infection caused by the anaerobic gram-negative rod fusobacterium nucleatum belongs to endogenous infections. a 41-year-old man developed multiple brain abscesses of odontogenic origin. the main causes were poor oral hygiene and no dental check-ups. pus aspired from a site in the brain contained dna of the bacterium fusobacterium nucleatum. several teeth with periapical abscesses were extracted. initially, the patient was treated empirically with a combination of cefotaxime and metronidazole (18 ... | 2008 | 18821484 |
| periodontal bacterial profiles in pregnant women: response to treatment and associations with birth outcomes in the obstetrics and periodontal therapy (opt) study. | a recent clinical trial (obstetrics and periodontal therapy [opt] study) demonstrated that periodontal therapy during pregnancy improved periodontal outcomes but failed to impact preterm birth. the present study evaluated seven target bacteria, aggregatibacter actinomycetemcomitans (previously actinobacillus actinomycetemcomitans), porphyromonas gingivalis, treponema denticola, tannerella forsythia (previously t. forsythensis), prevotella intermedia, campylobacter rectus, and fusobacterium nucle ... | 2008 | 18834241 |
| analysis of the antimicrobial activity of local anaesthetics used for dental analgesia. | seven local anaesthetics and their active anaesthetic components [ultracaine d-s (articaine hydrochloride), carbostesin (bupivacaine hydrochloride), scandicaine (mepivacaine hydrochloride), xylonest (prilocaine hydrochloride), xylocaine (lidocaine hydrochloride), hostacaine (butanilicaine phosphate) and novocaine (procaine hydrochloride)] were tested for their antimicrobial activity against 311 bacterial strains from 52 different species and 14 candida albicans strains. the tested pathogens were ... | 2008 | 18065672 |
| effects of age and oral disease on systemic inflammatory and immune parameters in nonhuman primates. | this report evaluated systemic inflammatory and immune biomarkers in a cohort of macaca mulatta (rhesus monkeys) maintained as a large family social unit, including an age range from <1 year to >24 years. we hypothesized that the systemic host responses would be affected by the age, gender, and clinical oral presentation of the population, each contributing to inflammatory and immune responses that would reflect chronic oral infections. the results demonstrated that the prevalence and severity o ... | 2008 | 18448617 |
| ability of oral bacteria to induce tissue-destructive molecules from human neutrophils. | the induction of tissue-destructive molecules from neutrophils by periodontopathic bacteria has been suggested as one of the mechanisms of periodontal destruction. the aim of this study was to determine whether the ability to stimulate neutrophils is an authentic characteristic of periodontopathic bacteria. | 2008 | 18449962 |
| involvement of phospholipase d in regulating expression of anti-microbial peptide human beta-defensin-2. | human beta-defensin expression correlates with differentiation in oral epithelium, and calcium ion, an important regulator of epithelial differentiation, plays a critical role in regulation of human beta-defensin-2 (hbd-2) mrna expression. phospholipase d (pld) also regulates epithelial differentiation. therefore, we examined the role of pld in hbd-2 up-regulation by cell wall extract of fusobacterium nucleatum and phorbol 12-myristate 13-acetate (pma), two known hbd-2 activators. we found that ... | 2008 | 17986621 |
| quercetin in a lotus leaves extract may be responsible for antibacterial activity. | in the course of a search for chemotherapeutic agents inhibiting suspected peridontitis bacteria, extracted and purified substances from lotus leaf were identified by antimicrobial activity tests with use of the broth micro-dilution methods on 96-microwell plate. the antimicrobial activity of extracts was tested against five microorganisms, namely: actinobacillus actinomycetemcomitans y4, actinomyces viscosus 19246, porphyromonas gingivalis 33277, fusobacterium nucleatum 25586, and actinomyces n ... | 2008 | 18481022 |
| photodynamic treatment of endodontic polymicrobial infection in vitro. | we investigated the photodynamic effects of methylene blue on multispecies root canal biofilms comprising actinomyces israelii, fusobacterium nucleatum subspecies nucleatum, porphyromonas gingivalis, and prevotella intermedia in experimentally infected root canals of extracted human teeth in vitro. the 4 test microorganisms were detected in root canals by using dna probes. scanning electron microscopy showed the presence of biofilms in root canals before therapy. root canal systems were incubate ... | 2008 | 18498901 |
| the anti-endotoxic effects of the ksl-w decapeptide on escherichia coli o55:b5 and various oral lipopolysaccharides. | host responses following the recognition of bacterial lipopolysaccharide can range from acute inflammation to septic shock. the aim of this study was to evaluate the ability of the ksl-w decapeptide to bind to and block the endotoxic effects of lipopolysaccharide. | 2008 | 18503516 |
| [periodontitis and cardiovascular disease]. | the prevalence of periodontitis and cardiovascular disease (cvd) is high. a mixed infectious biofilm etiology of periodontitis is known but not fully established in cvd. cofactors; smoking habits, stress, ethnicity, genetics, socioeconomics and age contribute to both diseases. the objectives of this report are to summarize factors in regards to cvd and periodontitis that are clinically relevant. the hypothesis behind a relationship between the two conditions can be founded in (i) shared infectio ... | 2008 | 18517068 |
| in vitro evaluation of the effect of nicotine, cotinine, and caffeine on oral microorganisms. | the aim of this in vitro study was to evaluate the effects of nicotine, cotinine, and caffeine on the viability of some oral bacterial species. it also evaluated the ability of these bacteria to metabolize those substances. single-species biofilms of streptococcus gordonii, porphyromonas gingivalis, or fusobacterium nucleatum and dual-species biofilms of s. gordonii -- f. nucleatum and f. nucleatum -- p. gingivalis were grown on hydroxyapatite discs. seven species were studied as planktonic cell ... | 2008 | 18535638 |
| pcr-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth. | the aim of the present study was to evaluate the presence of the selected pathogens in samples from deciduous and permanent tooth root canals by using pcr method and to determine the association of these organisms with clinical symptoms. | 2008 | 18547832 |
| coaggregation between and among human intestinal and oral bacteria. | coaggregation is believed to facilitate the integration of new bacterial species into polymicrobial communities. the aim of this study was to investigate coaggregation between and among human oral and enteric bacteria. stationary phase cultures of 10 oral and 10 enteric species, chosen on the basis of numerical and ecological significance in their respective environments together with their ease of cultivation, were tested using a quantitative spectrophotometric coaggregation assay in all possib ... | 2008 | 18554305 |
| experimental evidence supports the abscess theory of development of radicular cysts. | the objective of this study was to experimentally induce inflammatory cysts in an animal model so as to test the hypothesis that radicular cysts develop via the "abscess pathway." | 2008 | 18554947 |
| [relationship between herpesviruses and periodontal pathogenic bacteria in subgingival plaque]. | to evaluate the prevalence rates of human cytomegalovirus(hcmv) and epstein-barr virus-1(ebv-1) in subgingival plaque and analyze the relationship between herpesviruses, periodontal pathogenic bacteria and periodontal clinical parameters in chinese patients with aggressive periodontitis(agp). | 2008 | 18560463 |
| rapid and simple detection of eight major periodontal pathogens by the loop-mediated isothermal amplification method. | loop-mediated isothermal amplification (lamp) was applied to develop a rapid and simple detection system for eight periodontal pathogens: aggregatibacter (actinobacillus) actinomycetemcomitans, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, treponema denticola and tannerella forsythia. primers were designed from the 16s ribosomal rna gene for each pathogen, and the lamp amplified the targets specifically and efficiently under ... | 2008 | 18565108 |
| differential gender effects of a reduced-calorie diet on systemic inflammatory and immune parameters in nonhuman primates. | dietary manipulation, including caloric restriction, has been shown to impact host response capabilities significantly, particularly in association with aging. this investigation compared systemic inflammatory and immune-response molecules in rhesus monkeys (macaca mulatta). | 2008 | 18565132 |
| effects of er:yag laser irradiation on biofilm-forming bacteria associated with endodontic pathogens in vitro. | with the development of dental laser delivery systems that can enter into the root canals, it is possible to use er:yag lasers to remove the residual biofilm associated with infected root canals. we examined their effects against biofilms made of actinomyces naeslundii, enterococcus faecalis, lactobacillus casei, propionibacterium acnes, fusobacterium nucleatum, porphyromonas gingivalis, or prevotella nigrescens in vitro. after er:yag laser irradiation with energy densities ranging between 0.38- ... | 2008 | 18570988 |
| comparative analysis of endodontic pathogens using checkerboard hybridization in relation to culture. | the purpose of this study was to detect bacterial species and to quantify the total number of bacteria from samples of infected root canals before (s1) and after chemo-mechanical preparation using 2% chlorhexidine (chx) gel as auxiliary chemical substance (s2) and after 7 days of intracanal dressing (s3) to compare microbial changes. | 2008 | 18582327 |
| periodontal status of patients with dentin dysplasia type i: report of three cases within a family. | dentin dysplasia type i (ddi) is a rare hereditary disturbance of dentin formation. it is characterized by clinically normal-appearing crowns; obliteration of pulp chambers; and short, blunted and malformed roots that are commonly associated with periodontal attachment loss (pal). in this context, we report three cases within a family with similar clinical and radiographic features of ddi but with differing microbiologic and periodontal conditions. | 2008 | 18597615 |
| biochemical characterization of a glycosyltransferase homolog from an oral pathogen fusobacterium nucleatum as a human glycan-modifying enzyme. | bacterial glycosyltransferases have drawn growing attention as economical enzymes for oligosaccharide synthesis, with their easy expression and relatively broad substrate specificity. here, we characterized a glycosyltransferase homolog (fnu_gt) from a human oral pathogen, fusobacterium nucleatum. bioinformatic analysis showed that fnu_gt belongs to the glycosyltransferases family ii. the recombinant fnu_gt (rfnu_gt) expressed in escherichia coli displayed the highest glycosylation activity when ... | 2008 | 18633282 |
| third molars and the efficacy of mechanical debridement in reducing pathogen levels in pregnant subjects: a pilot study. | to assess the impact of mechanical debridement (scaling and root planing), without adjunctive therapy, on reducing the numbers of periodontal pathogens detected in pregnant subjects with and without visible third molars. | 2008 | 18634941 |
| protein-lipid interactions with fusobacterium nucleatum major outer membrane protein foma: spin-label epr and polarized infrared spectroscopy. | foma, the major outer membrane protein of fusobacterium nucleatum, was expressed and purified in escherichia coli and reconstituted from detergent in bilayer membranes of phosphatidylcholines with chain lengths from c(12:0) to c(17:0). the conformation and orientation of membrane-incorporated foma were determined from polarized, attenuated total reflection, infrared (ir) spectroscopy, and lipid-protein interactions with foma were characterized by using electron paramagnetic resonance (epr) spect ... | 2008 | 18642853 |
| [study of adherence of periodontal pathogens to collagen-treated hydroxyapatite]. | the ability of oral bacteria to adhere to tooth surface is associated with their pathogenicity. the objective of this study was to compare the ability of 4 strains of periodontal pathogens attaching to collagen-treated hydroxyapatite (c-ha) beads in order to evaluate the ability of the main periodontal pathogens to form the biofilm on root surface. | 2008 | 18705525 |
| antiviral, antibacterial and vitamin c-synergized radical-scavenging activity of sasa senanensis rehder extract. | sasa senanensis rehder extract (se) showed slightly higher cytotoxicity against human squamous cell carcinoma cell lines and human glioblastoma cell lines, as compared with human oral normal cells (gingival fibroblast, pulp cell, periodontal ligament fibroblast), and was more cytotoxic to human myelogenous and t-cell leukemia cell lines. se showed a bacteriostatic effect on fusobacterium nucleatum and prevotella intermedia, but almost completely eliminated hydrogen sulfide (h2s) and methyl merca ... | 2008 | 18712174 |
| the influence of mineral trioxide aggregate on adaptive immune responses to endodontic pathogens in mice. | this study assessed the influence of mineral trioxide aggregate (mta) on adaptive immune responses. balb/c mice were immunized with heat-killed fusobacterium nucleatum (fn) in mta or other control adjuvants, and serum igg responses to fn were measured. either fn- or peptostreptococcus anaerobius (pa)-reactive memory t cells (tm) were preincubated in vitro with/without mta and restimulated with fn or pa. tm proliferation and cytokine production were assessed. compared with control groups, immunog ... | 2008 | 18718367 |
| endodontic microorganism susceptibility by direct contact test. | the aim of this study was to evaluate in vitro the duration of the antimicrobial effect of endodontic sealers by means of the direct contact test. the sealers tested were: endomethasone - septodont, endomethasone c-septodont, endion-voco, diaket-espe, pulp canal sealer-sybronendo, and ah26-dentsply detrey. the endodontopathic microorganisms (mo) confronted were: staphylococcus aureus (sa), candida albicans (ca), enterococcus faecalis (ef), prevotella intermedia (pi), porphyromonas gingivalis (pg ... | 2008 | 19177855 |
| effect of xylitol on an in vitro model of oral biofilm. | the aim of the present study was to examine whether xylitol, at different concentrations, inhibits the formation of an experimental model of oral biofilm. | 2008 | 19178100 |
| [anaerobic gram-negative bacilli involved in the etiopathogeny of the abscesses of superficial fascial spaces of the face and neck]. | the aim of this study was to isolate and identify at species level the strains of anaerobic gram-negative bacilli (gnb) from pus samples collected in patients with abscesses of fascial spaces of the face and neck. microscopy of gram-stained smears and cultures were performed in each specimen. the strictly anaerobic gnb strains were identified using the conventional methods of diagnosis and the rapid id 32 a system. in addition, the other strains isolated in association with these bacteria were i ... | 2008 | 20201270 |
| bacterial leakage along the implant-abutment interface of premachined or cast components. | in recent clinical studies, contamination of the inner parts of dental implants through bacterial penetration along the implant components has been observed. the aim of the present in-vitro study was to investigate leakage of fusobacterium nucleatum through the interface between implants and premachined or cast abutments. both premachined (n=10) and cast (n=10) implant-abutment assemblies were inoculated with 3.0 microl of microbial inoculum. the assemblies were completely immersed in 5.0 ml of ... | 2008 | 17931833 |
| effect of photo-activated disinfection on endodontic pathogens ex vivo. | to test the hypothesis that photo-activated disinfection (pad) has a bactericidal effect on pathogens inoculated in root canals, with emphasis on biofilm formation/destruction. | 2008 | 18081808 |
| quantitative analysis of association between herpesviruses and bacterial pathogens in periodontitis. | the development of human periodontitis may depend upon cooperative interactions among herpesviruses, specific pathogenic bacteria and tissue-destructive inflammatory mediators. this study sought to identify associations among human cytomegalovirus, epstein-barr virus and six putative periodontopathic bacteria in periodontitis lesions. | 2008 | 18086168 |
| stimulation of fusobacterium nucleatum biofilm formation by porphyromonas gingivalis. | bacterial infection is a major cause of periapical periodontitis. eradication of these microorganisms from apical lesions is essential to the success of endodontic treatment. the aim of this study was to clarify the molecular interaction between fusobacterium nucleatum, porphyromonas gingivalis and other microorganisms associated with periapical periodontitis. | 2008 | 18173791 |
| proteomic analysis of ampicillin-resistant oral fusobacterium nucleatum. | fusobacterium nucleatum represents one of the predominant anaerobic species in the oral microbiota. penicillin-resistant f. nucleatum have been isolated from intra- and extraoral infections. this study aimed to assess ampicillin resistance in f. nucleatum by investigating the synthesis of resistance-associated proteins. | 2008 | 18173796 |
| relationship between campylobacter rectus and periodontal status during pregnancy. | in a previous study, we showed that the growth of campylobacter rectus is stimulated by the presence of female sex hormones in the culture medium. in the present study, we examined the relationship between c. rectus levels in the saliva and the periodontal status of pregnant women. | 2008 | 18173799 |
| microflora in oral ecosystems in subjects with radiation-induced hyposalivation. | to analyse the microbial flora in specific oral sites in 13 dentate subjects, 6-8 months after completed radiation therapy (rt group) and in 13 matched controls. | 2008 | 18208474 |
| genetic and biochemical characterization of oxa-63, a new class d beta-lactamase from brachyspira pilosicoli bm4442. | brachyspira pilosicoli bm4442, isolated from the feces of a patient with diarrhea at the hospital saint-michel in paris, was resistant to oxacillin (mic > 256 microg/ml) but remained susceptible to cephalosporins and to the combination of amoxicillin and clavulanic acid. cloning and sequencing of the corresponding resistance determinant revealed a coding sequence of 807 bp encoding a new class d beta-lactamase named oxa-63. the bla oxa-63 gene was chromosomally located and not part of a transpos ... | 2008 | 18212108 |
| in vitro antioxidant potential of medicinal plant extracts and their activities against oral bacteria based on brazilian folk medicine. | this study aims to determine antibacterial activities of cocos nucifera (husk fiber), ziziphus joazeiro (inner bark), caesalpinia pyramidalis (leaves), aqueous extracts and aristolochia cymbifera (rhizomes) alcoholic extract against prevotella intermedia, porphyromonas gingivalis, fusobacterium nucleatum, streptococcus mutans and lactobacillus casei. the antioxidant activity and acute toxicity of these extracts were also evaluated. | 2008 | 18243157 |
| a case report of a pyogenic liver abscess caused by fusobacterium nucleatum in a patient with autosomal dominant polycystic kidney disease undergoing hemodialysis. | pyogenic liver abscess (pla) is a process with significant morbidity and mortality and is a rare complication in an aisled way in patients with autosomal dominant polycystic kidney disease (adpkd). in addition to hepatic cyst infection, intracystic hemorrhage is another complication seen in adpkd patients; however, the liver parenchyma itself remains normal. a pla located in normal liver tissue in these kinds of patients has not been previously reported. fusobacterium nucleatum is an anaerobic b ... | 2008 | 18257820 |
| smap29 congeners demonstrate activity against oral bacteria and reduced toxicity against oral keratinocytes. | cathelicidins are antimicrobial peptides found in epithelial and mucosal tissues as well as the secondary granules of neutrophils. smap29, a sheep cathelicidin, has differential antimicrobial properties against various pathogens, including periodontal organisms. the purpose of this study was to evaluate the antimicrobial properties and cytotoxicity of smap29, smap28, and three congeners (smap18a, smap18d, and smap14a). | 2008 | 18279175 |
| aggregatibacter actinomycetemcomitans serotype f o-polysaccharide mediates coaggregation with fusobacterium nucleatum. | intergeneric bacterial coaggregation may play an important role in plaque development. | 2008 | 18279180 |
| colonization pattern of periodontal bacteria in japanese children and their mothers. | the purpose of this study was to determine the time of infection by anaerobic gram-negative rods associated with periodontal disease, and to clarify their transmission from mother to child. | 2008 | 18302616 |