Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| biotransformation of adenine and cytokinins by the rhizobacterium serratia proteamaculans. | approximately 60,000 microorganisms from saskatchewan soil were screened for growth on the cytokinin n6-benzyladenine (ba) as c source. a single isolate, identified as serratia proteamaculans, grew well on ba. the culture filtrates from s. proteamaculans were screened using reversed phase high performance liquid chromatography (rp-hplc) for the presence of secondary metabolites. the analysis revealed a major metabolite and its chemical structure was deduced as 8-hydroxy-n6-benzyladenine (8-ohba) ... | 2006 | 16860349 |
| engineering tce-degrading rhizobacteria for heavy metal accumulation and enhanced tce degradation. | many superfund sites are currently co-contaminated with organic pollutants such as trichloroethene (tce) and heavy metals. a promising strategy to address these mixed-waste situations is the use of tce-degrading rhizobacteria that will survive and thrive in soil heavily polluted with heavy metals. in this work, a gene coding for the metal-binding peptide, ec20, was introduced into rhizobacteria engineered for tce degradation, resulting in strains with both metal accumulation and tce degradation ... | 2006 | 16862598 |
| establishment of salvia officinalis l. hairy root cultures for the production of rosmarinic acid. | shoots of salvia officinalis, a medicinally important plant, were infected with agrobacterium rhizogenes strains atcc 15834 and a4 which led to the induction of hairy roots in 57% and 37% of the explants, respectively. seven lines of hairy roots were established in wp liquid medium under light and dark conditions. the transformed nature of the root lines was confirmed by polymerase chain reaction using rolb and rolc specific primers. transformed root cultures of salvia officinalis showed variati ... | 2006 | 16869492 |
| sennosides a and b production by hairy roots of senna alata (l.) roxb. | hairy roots of senna alata transformed with agrobacterium rhizogenes, strain atcc 15834 were induced and grown in half-strength murashige and skoog (ms) medium. effects of sucrose contents and hormones on the growth and sennosides a, b production were investigated. hairy roots cultured on hormone-free half-strength ms medium containing 5% sucrose under dark condition mostly stimulated the growth of hairy roots and increased the content of sennosides a and b yielding (169 +/- 4) and (34 +/- 3) mi ... | 2006 | 16869495 |
| harnessing the potential of hairy roots: dawn of a new era. | in the past two decades, hairy root research for the production of important secondary metabolites has received a lot of attention. the addition of knowledge to overcome the limiting culture parameters of the regulation of the metabolic pathway by specific molecules and the development of novel tools for metabolic engineering now offer new possibilities to improve the hairy root technique for the production of metabolites. furthermore, engineering hairy roots for the production of animal protein ... | 2006 | 16870285 |
| [chemical composition and immunochemical characteristics of the lipopolysaccharide of nitrogen-fixing rhizobacterium azospirillum brasilense cd]. | the chemical composition of the lipopolysaccharide of the associative diazotrophic rhizobacterium azospirillum brasilense cd has been studied. among the main components of the hydrophobic part of the lipopolysaccharide, we identified 3-hydroxytetradecanoic, hexadecenoic, 3-hydroxyhexadecanoic, hexadecanoic, octadecenoic, and nanodecanoic fatty acids; the carbohydrate part contained rhamnose, galactose, and mannose. polyclonal antibodies against the preparation under study were raised in rabbits. ... | 2006 | 16871806 |
| refinement of the citrus tristeza virus resistance gene (ctv) positional map in poncirus trifoliata and generation of transgenic grapefruit (citrus paradisi) plant lines with candidate resistance genes in this region. | citrus tristeza virus (ctv) is a major pathogen of citrus. a single dominant gene ctv present in the trifoliate relative of citrus, poncirus trifoliata confers broad spectrum resistance against ctv. refinement of genetic maps has delimited this gene to a 121 kb region, comprising of ten candidate ctv resistance genes. the ten candidate genes were individually cloned in agrobacterium based binary vector and transformed into three ctv susceptible grapefruit varieties. two of the candidate r-genes, ... | 2006 | 16830176 |
| insights into recognition of the t-dna border repeats as termination sites for t-strand synthesis by agrobacterium tumefaciens. | the recognition of the t-dna left border (lb) repeat is affected by its surrounding sequences. here, the lb regions were further characterized by molecular analysis of transgenic plants, obtained after agrobacterium tumefaciens-mediated transformation with t-dna vectors that had been modified in this lb region. at least the 24-bp lb repeat by itself was insufficient to terminate the t-strand synthesis. addition of the natural inner and/or outer border regions to at least the lb repeat, even when ... | 2006 | 16830227 |
| transformation of barley (hordeum vulgare l.) by agrobacterium tumefaciens infection of in vitro cultured ovules. | we report on a novel transformation procedure for barley by agrobacterium infection of in vitro cultured ovules. ovules of the cultivar golden promise were isolated a few hours after pollination and infected with the agrobacterium tumefaciens strain agl0 carrying the binary vector pvec8-gfp. the vector harboured a hygromycin resistance gene and the green fluorescence protein (gfp) gene. gfp-expressing embryos were isolated from the ovules, regenerated to plants and investigated by southern blot ... | 2006 | 16832622 |
| peddling the nitrogen cycle. | 2006 | 16835960 | |
| factors contributing to deletion within mungbean yellow mosaic virus partial dimers in binary vectors used for agroinoculation. | mungbean yellow mosaic virus-vigna (mymv) sequences cloned as partial dimers within the t-dna of a binary vector were deleted at a high frequency upon conjugal mobilization from escherichia coli into agrobacterium tumefaciens. this deletion involving the genome-length viral dna did not occur when the binary plasmid was inside e. coli and when the binary plasmid was introduced into agrobacterium by electroporation. deletions occurred in both dna a and dna b partial dimers. a minimum of 500-nt con ... | 2006 | 16837070 |
| genetic transformation of centaurium erythraea rafn by agrobacterium rhizogenes and the production of secoiridoids. | hairy roots of centaurium erythraea were obtained by infection with agrobacterium rhizogenes strain lba 9402. they spontaneously regenerated adventitious shoots in woody plant liquid medium without growth regulators. the shoots were grown continuously in murashige and skoog (ms) liquid or agar solidified media supplemented with 0.1 mg l(-1) indole-3-acetic acid and 1.0 mg l(-1) 6-benzylaminopurine. these shoots produced roots 4 weeks after transfer into agar-solidified ms medium without phytohor ... | 2006 | 16841219 |
| isolation and characterization of functional insertion sequences of rhizobia. | rhizobia are a group of bacteria that form nodules on the roots of legume host plants. the sequenced genomes of the rhizobia are characterized by the presence of many putative insertion sequences (is) elements. however, it is unknown whether these is elements are functional and it is therefore relevant to assess their transposition activity. in this work, several functional insertion sequences belonging to the is1256, is3, is5, is166, and is21 families were captured from rhizobium tropici, rhizo ... | 2006 | 16842354 |
| accumulation of glycinebetaine in rice plants that overexpress choline monooxygenase from spinach and evaluation of their tolerance to abiotic stress. | glycinebetaine (gb), a quaternary ammonium compound, is a very effective compatible solute. in higher plants, gb is synthesized from choline (cho) via betaine aldehyde (ba). the first and second steps in the biosynthesis of gb are catalysed by choline monooxygenase (cmo) and by betaine aldehyde dehydrogenase (badh), respectively. rice (oryza sativa), which has two genes for badh, does not accumulate gb because it lacks a functional gene for cmo. rice plants accumulate gb in the presence of exoge ... | 2006 | 16790464 |
| proteomic analysis of agrobacterium tumefaciens response to the vir gene inducer acetosyringone. | agrobacterium tumefaciens causes crown gall disease in a wide range of plants by transforming plants through the transfer and integration of its transferred dna (t-dna) into the host genome. in the present study, we used two-dimensional gel electrophoresis to examine the protein expression profiles of a. tumefaciens in response to the phenolic compound acetosyringone (as), a known plant-released virulence (vir) gene inducer. using mass spectrometry, we identified 11 proteins consisting of 9 know ... | 2006 | 16791832 |
| [effect of lox-sites of the cre lox recombination system on promoterless bar gene expression in transgenic plants]. | we demonstrate that localization of lox site between the right border of t-dna and promoterless bar gene (rb-lox-bar-) led to its highly efficient expression in transgenic plants of nicotiana tabacum and n. africana. plasmid vectors used in gene integration experiments contained neomycin phosphotransferase ii (npt ii) gene under nos promoter as well. transgenic plants were selected according to their capacity to grow on the medium with kanamycin and then they were tested on the selective medium ... | 2006 | 16792015 |
| detection of genetic modification in cured tobacco leaf: proficiency testing using polymerase chain reaction-based methods. | the cooperation centre for scientific research relative to tobacco (coresta; paris, france) "task force genetically modified tobacco-detection methods" investigated the performance of qualitative and quantitative methods based on the polymerase chain reaction (pcr) for the detection and quantitation of genetically modified (gm) tobacco. in the 4 successful rounds of proficiency testing, the cauliflower mosaic virus 35s rna promoter (camv 35s) and the agrobacterium tumefaciens nopaline synthase t ... | 2006 | 16792068 |
| a novel function for a ubiquitous plant enzyme pectin methylesterase: the enhancer of rna silencing. | co-agroinjection of nicotiana benthamiana leaves with the pectin methylesterase (propme) gene and the tmv:gfp vector resulted in a stimulation of virus-induced rna silencing (inhibition of gfp production, virus rna degradation, stimulation of sirnas production). conversely, co-expression of tmv:gfp with either antisense pme construct or with enzymatically inactive propme restored synthesis of viral rna. furthermore, expression of propme enhanced the gfp transgene-induced gene silencing accompani ... | 2006 | 16797009 |
| purification of coenzyme q10 from fermentation extract: high-speed counter-current chromatography versus silica gel column chromatography. | high-speed counter-current chromatography (hsccc) is applied to the purification of coenzyme q(10) (coq(10)) for the first time. coq(10) was obtained from a fermentation broth extract. a non-aqueous two-phase solvent system composed of heptane-acetonitrile-dichloromethane (12:7:3.5, v/v/v) was selected by analytical hsccc and used for purification of coq(10) from 500 mg of the crude extract. the separation yielded 130 mg of coq(10) at an hplc purity of over 99%. the overall results of the presen ... | 2006 | 16797569 |
| functional characterization of a novel hydrocarbonoclastic pseudomonas sp. strain pup6 with plant-growth-promoting traits and antifungal potential. | a novel hydrocarbonoclastic bacterium was isolated from rice rhizospheric soil using an enrichment culture technique. detailed taxonomic studies identified the organism, designated strain pup6, as a member of the genus pseudomonas. the bacterium grew in minimal medium amended with n-alkane members of hydrocarbons, n-dodecane (c12h26), n-hexadecane (c16h34), n-octadecane (c18h38), n-octacosane (c28h58); and petroleum fractions such as crude oil and lubricating oil when provided as sole carbon and ... | 2006 | 16797931 |
| agrobacterium-mediated transformation of cotton (gossypium hirsutum l. cv. zhongmian 35) using glyphosate as a selectable marker. | the most economically significant chinese cotton cultivar (gossypium hirsutum l. cv. zhongmian 35) was transformed via agrobacterium tumefaciens-mediated dna transfer. the aroa-m1 gene that confers resistance to the glyphosate was fused with a chloroplast-transit peptide of arabidopsis thaliana 5-enolpyruvyl-3-phosphoshikimate synthase (asp) and expressed in cotton plants under the control of a camv35s promoter. transgenic plants were directly selected on medium containing glyphosate. thirty-fou ... | 2006 | 16799756 |
| agrobacterium tumefaciens-mediated transformation of rhipsalidopsis gaertneri. | a protocol for agrobacterium tumefaciens-mediated genetic transformation of rhipsalidopsis cv. cb5 was developed. calluses derived from phylloclade explants and sub-cultured onto fresh callus induction medium over a period of 9-12 months were co-cultivated with a. tumefaciens lba4404. plasmid constructs carrying the nptii gene, as a selectable marker, and the reporter uida gene were used. transformed rhipsalidopsis calluses with a vigorous growth phenotype were obtained by extended culture on me ... | 2006 | 16799807 |
| metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase. | potato is a major staple food, and modification of its provitamin content is a possible means for alleviating nutritional deficiencies. beta-carotene is the main dietary precursor of vitamin a. potato tubers contain low levels of carotenoids, composed mainly of the xanthophylls lutein, antheraxanthin, violaxanthin, and of xanthophyll esters. none of these carotenoids have provitamin a activity. | 2006 | 16800876 |
| transgenic peas (pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (rubus idaeus) and stilbene synthase from grape (vitis vinifera). | the pea (pisum sativum l.) varieties baroness (united kingdome) and baccara (france) were transformed via agrobacterium tumefaciens-mediated gene transfer with pgptv binary vectors containing the bar gene in combination with two different antifungal genes coding for polygalacturonase-inhibiting protein (pgip) from raspberry (rubus idaeus l.) driven by a double 35s promoter, or the stilbene synthase (vst1) from grape (vitis vinifera l.) driven by its own elicitor-inducible promoter. transgenic li ... | 2006 | 16802117 |
| phylogeny of nodulation genes and symbiotic properties of genista tinctoria bradyrhizobia. | pairwise comparisons of genista tinctoria (dyer's weed) rhizobium noda, nodc, and nodz gene sequences to those available in databanks revealed their highest sequence identities to nodulation loci of bradyrhizobium sp. (lupinus) strains and rhizobia from other genistoid legumes. on phylogenetic trees, genistoid microsymbionts were grouped together in monophyletic clusters, which suggested that their nodulation genes evolved from a common ancestor. g. tinctoria nodulators formed symbioses not only ... | 2006 | 16802175 |
| assembly of synthetic locked chromophores with agrobacterium phytochromes agp1 and agp2. | phytochromes are photoreceptors with a bilin chromophore in which light triggers the conversion between the red-absorbing form pr and the far-red-absorbing form pfr. agrobacterium tumefaciens has two phytochromes, agp1 and agp2, with antagonistic properties: in darkness, agp1 converts slowly from pfr to pr, whereas agp2 converts slowly from pr to pfr. in a previous study, we have assembled agp1 with synthetic locked chromophores 15za, 15zs, 15ea, and 15es in which the c15=c16 double bond is fixe ... | 2006 | 16803878 |
| characterization of genes involved in erythritol catabolism in rhizobium leguminosarum bv. viciae. | a genetic locus encoding erythritol uptake and catabolism genes was identified in rhizobium leguminosarum bv. viciae, and shown to be plasmid encoded in a wide range of r. leguminosarum strains. a tn5-b22 mutant (19b-3) unable to grow on erythritol was isolated from a mutant library of r. leguminosarum strain vf39sm. the mutated gene eryf was cloned and partially sequenced, and determined to have a high homology to permease genes of abc transporters. a cosmid complementing the mutation (pcos42) ... | 2006 | 16804181 |
| expression of the autofluorescent protein, dsred2, in the recombinants of the ectomycorrhizal basidiomycete, suillus grevillei, generated by agrobacterium-mediated transformation. | recombinants were generated from the ectomycorrhizal basidiomycete, suillus grevillei, through agroinfection using a binary vector carrying the hygromycin b resistance and the autofluorescent protein, dsred2, markers. dsred2 was driven by a cis-regulatory region of the glyceraldeyde-3-phosphate dehydrogenase gene (gpd) from the wood-rotting basidiomycete, coriolus hirsutus, which contains promoters and 5' gpd sequences with first through fourth exons and expressed for the first time in suillus s ... | 2006 | 16804705 |
| transgenic acacia sinuata from agrobacterium tumefaciens-mediated transformation of hypocotyls. | transgenic herbicide tolerant acacia sinuata plants were produced by transformation with the bar gene conferring phosphinothricin resistance. precultured hypocotyl explants were infected with agrobacterium tumefaciens strain eha105 in the presence of 100 microm acetosyringone and shoots regenerated on ms (murashige and skoog, 1962, physiol plant 15:473-497) medium with 13.3 microm benzylaminopurine, 2.6 microm indole-3-acetic acid, 1 g l(-1) activated charcoal, 1.5 mg l(-1) phosphinothricin, and ... | 2006 | 16807750 |
| genetic transformation of peanut (arachis hypogaea l.) using cotyledonary node as explant and a promoterless gus::nptii fusion gene based vector. | we have generated putative promoter tagged transgenic lines in arachis hypogaea cv jl-24 using cotyledonary node (cn) as an explant and a promoterless gus::nptii bifunctional fusion gene mediated by agrobacterium transformation. ms medium fortified with 6-benzylaminopurine (bap) at 4mg/l in combination with 0.1 mg/l alpha -napthaleneacetic acid (naa) was the most effective out of the various bap and naa combinations tested in multiple shoot bud formation. parameters enhancing genetic transformat ... | 2006 | 16809856 |
| nodulation independent of rhizobia induced by a calcium-activated kinase lacking autoinhibition. | legumes, such as medicago truncatula, form mutualistic symbiotic relationships with nitrogen-fixing rhizobial bacteria. this occurs within specialized root organs--nodules--that provide the conditions required for nitrogen fixation. a rhizobium-derived signalling molecule, nod factor, is required to establish the symbiosis. perception of nod factor in the plant leads to the induction of ca2+ oscillations, and the transduction of this ca2+ signal requires dmi3 (refs 2, 3), which encodes the prote ... | 2006 | 16810256 |
| constitutive activation of two-component response regulators: characterization of virg activation in agrobacterium tumefaciens. | response regulators are the ultimate modulators in two-component signal transduction pathways. the n-terminal receiver domains generally accept phosphates from cognate histidine kinases to control output. virg for example, the response regulator of the vira/virg two-component system in agrobacterium tumefaciens, mediates the expression of virulence genes in response to plant host signals. response regulators have a highly conserved structure and share a similar conformational activation upon pho ... | 2006 | 16816192 |
| rnai knock-down of enod40s leads to significant suppression of nodule formation in lotus japonicus. | enod40 is one of the most intriguing early nodulin genes that is known to be induced very early in response to interaction of legume plants with symbiotic rhizobium bacteria, but its function in the nodulation process is still not known. lotus japonicus has two enod40 genes: ljenod40-1 is abundantly induced in very early stages of bacterial infection or nod factor application, whereas ljenod40-2 is abundantly expressed only in mature nodules. we generated transgenic lines of l. japonicus with an ... | 2006 | 16816411 |
| postgenomic scan of metallo-beta-lactamase homologues in rhizobacteria: identification and characterization of bjp-1, a subclass b3 ortholog from bradyrhizobium japonicum. | the diffusion of metallo-beta-lactamases (mbls) among clinically important human pathogens represents a therapeutic issue of increasing importance. however, the origin of these resistance determinants is largely unknown, although an important number of proteins belonging to the mbl superfamily have been identified in microbial genomes. in this work, we analyzed the distribution and function of genes encoding mbl-like proteins in the class rhizobiales. among 12 released complete genomes of member ... | 2006 | 16723554 |
| bases of biocontrol: sequence predicts synthesis and mode of action of agrocin 84, the trojan horse antibiotic that controls crown gall. | agrobacterium radiobacter k84, used worldwide to biocontrol crown gall disease caused by agrobacterium tumefaciens, produces an antiagrobacterial compound called agrocin 84. we report the nucleotide sequence of pagk84, a 44.42-kb plasmid coding for production of this disubstituted adenine nucleotide antibiotic. pagk84 encodes 36 orfs, 17 of which (agn) code for synthesis of or immunity to agrocin 84. two genes, agnb2 and agna, encode aminoacyl trna synthetase homologues. we have shown that the t ... | 2006 | 16731618 |
| in vitro regeneration and agrobacterium-mediated genetic transformation of euonymus alatus. | an in vitro plant regeneration method and an agrobacterium tumefaciens-mediated genetic transformation protocol were developed for euonymus alatus. more than 60% of cotyledon and 70% of hypocotyl sections from 10-day-old seedlings of e. alatus produced 2-4 shoots on woody plant medium (wpm) supplemented with 5.0 mg/l 6-benzylaminopurine (ba) plus 0.2 mg/l alpha-naphthalene acetic acid (naa), and 77% of shoots produced roots on wpm medium with 0.3 mg/l naa and 0.5 mg/l indole-3-butyricacid (iba). ... | 2006 | 16733742 |
| functional effects of amino acid substitutions within the large binding pocket of the phosphotriesterase opda from agrobacterium sp. p230. | the phosphotriesterase opda from agrobacterium sp. p230 has about 10-fold higher activity for dimethyl organophosphate (op) insecticides, than its homologue from flavobacterium sp. atcc27551, organophosphate hydrolase (oph). opda shows about 10% amino acid sequence divergence from oph and also has a 20 residue c-terminal extension. here we show that the difference in kinetics is largely explained by just two amino acid differences between the two proteins. a truncated form of opda demonstrated t ... | 2006 | 16734778 |
| synchronization, transformation, and cryopreservation of suspension-cultured cells. | we have recently described the selection of rapidly dividing arabidopsis cell suspension cultures mm1 and mm2d that provide a powerful platform for plant cell-cycle research. here we provide detailed protocols and procedures to achieve high levels of synchronization, either by starving the cell cultures of sucrose or by applying the toxin aphidicolin. cell-cycle activity during cell-cycle reentry (starvation-induced synchrony) or further cell-cycle progression (aphidicolin-induced synchrony) can ... | 2006 | 16739567 |
| in planta agrobacterium-mediated transformation by vacuum infiltration. | in planta agrobacterium-mediated transformation using vacuum infiltration results in transgenic arabidopsis thaliana without the use of sterile conditions or plant regeneration. plants are grown in pots, in standard potting mix. agrobacterium tumefaciens, carrying an appropriate plant transformation vector, is suspended in an infiltration medium that contains, at a minimum, sucrose and the surfactant silwet l-77. flower buds are immersed in the suspension of a. tumefaciens. the application of a ... | 2006 | 16739579 |
| transient expression assay by agroinfiltration of leaves. | an agrobacterium-mediated transient expression assay has been described for in vivo analysis of constitutive or inducible gene expression in arabidopsis plants. by simple infiltration of agrobacterium cells carrying appropriate gene constructs into arabidopsis leaves, transient expression assays can be performed within 3 d without using expensive instruments or complicated procedures. two days after agroinfiltration, arabidopsis plants can be treated with chemical inducers such as dexamethasone ... | 2006 | 16739580 |
| proteome changes after metabolic engineering to enhance aerobic mineralization of cis-1,2-dichloroethylene. | metabolically engineered escherichia coli has previously been used to degrade cis-1,2-dichloroethylene (cis-dce). the strains express the six genes of an evolved toluene ortho-monooxygenase from burkholderia cepacia g4 (tom-green, which formed a reactive epoxide) with either (1) gamma-glutamylcysteine synthetase (gshi, which forms glutathione) and the glutathione s-transferase isoilr1 from rhodococcus ad45 (which adds glutathione to the reactive cis-dce epoxide) or (2) with an evolved epoxide hy ... | 2006 | 16739990 |
| transgenic expression of gallerimycin, a novel antifungal insect defensin from the greater wax moth galleria mellonella, confers resistance to pathogenic fungi in tobacco. | a cdna encoding gallerimycin, a novel antifungal peptide from the greater wax moth galleria mellonella, was isolated from a cdna library of genes expressed during innate immune response in the caterpillars. upon ectopic expression of gallerimycin in tobacco, using agrobacterium tumefaciens as a vector, gallerimycin conferred resistance to the fungal pathogens erysiphe cichoracearum and sclerotinia minor. quantification of gallerimycin mrna in transgenic tobacco by real-time pcr confirmed transge ... | 2006 | 16740126 |
| proteins exported via the prsd-prse type i secretion system and the acidic exopolysaccharide are involved in biofilm formation by rhizobium leguminosarum. | the type i protein secretion system of rhizobium leguminosarum bv. viciae encoded by the prsd and prse genes is responsible for secretion of the exopolysaccharide (eps)-glycanases plya and plyb. the formation of a ring of biofilm on the surface of the glass in shaken cultures by both the prsd and prse secretion mutants was greatly affected. confocal laser scanning microscopy analysis of green-fluorescent-protein-labeled bacteria showed that during growth in minimal medium, r. leguminosarum wild ... | 2006 | 16740954 |
| crystallization and preliminary x-ray analysis of an exotype alginate lyase atu3025 from agrobacterium tumefaciens strain c58, a member of polysaccharide lyase family 15. | almost all alginate lyases depolymerize alginate in an endolytical fashion via a beta-elimination reaction. the alginate lyase atu3025 from agrobacterium tumefaciens strain c58, consisting of 776 amino-acid residues, is a novel exotype alginate lyase classified into polysaccharide lyase family 15. the enzyme was crystallized at 293 k by sitting-drop vapour diffusion with polyethylene glycol 4000 as a precipitant. preliminary x-ray analysis showed that the atu3025 crystal belonged to space group ... | 2006 | 16682783 |
| factors affecting the attachment of rhizospheric bacteria to bean and soybean roots. | the plant rhizosphere is an important soil ecological environment for plant-microorganism interactions, which include colonization by a variety of microorganisms in and around the roots that may result in symbiotic, endophytic, associative, or parasitic relationships within the plant, depending on the type of microorganisms, soil nutrient status, and soil environment. rhizosphere competence may be attributable to the differences in the extent of bacterial attachment to the root surface. we prese ... | 2006 | 16684104 |
| [adsorption mechanism of chromium cation by floc-type biosorbent zl 5-2]. | biosorption of cr(vi) by the novel biosorbent zl5-2 produced by agrobacterium sp. was studied and infrared spectra of native, absorbing and desorbing zl 5-2 were compared. the initial ph was important for biosorption. the optimum effect on cr(vi) biosorption was acquired within initial ph 0.5 to approximately 1.5, then with the ph increasing, the effect on cr6+ biosorption was reduced. the biosorption for cr(vi) was a quick process,only within 10 min 65.4% cr(vi) is biosorbed, and the biosorptio ... | 2006 | 16686202 |
| anomalous scattering analysis of agrobacterium radiobacter phosphotriesterase: the prominent role of iron in the heterobinuclear active site. | bacterial phosphotriesterases are binuclear metalloproteins for which the catalytic mechanism has been studied with a variety of techniques, principally using active sites reconstituted in vitro from apoenzymes. here, atomic absorption spectroscopy and anomalous x-ray scattering have been used to determine the identity of the metals incorporated into the active site in vivo. we have recombinantly expressed the phosphotriesterase from agrobacterium radiobacter (opda) in escherichia coli grown in ... | 2006 | 16686603 |
| o-acetylation of the terminal n-acetylglucosamine of the lipooligosaccharide inner core in neisseria meningitidis. influence on inner core structure and assembly. | o-acetylation is a common decoration on endotoxins derived from many gram-negative bacterial species, and it has been shown to be instrumental (e.g. in salmonella typhimurium) in determining the final tertiary structure of the endotoxin and the immunogenicity of the molecule. structural heterogeneity of endotoxins produced by mucosal pathogens such as neisseria meningitidis is determined by decorations on the heptose inner core, including o-acetylation of the terminal n-acetylglucosamine (glcnac ... | 2006 | 16687398 |
| molecular diversity of rhizobia nodulating the invasive legume cytisus scoparius in australia. | to contribute to the understanding of cytisus scoparius success at invading and establishing itself in australia. | 2006 | 16696670 |
| antioxidative enzymes from chloroplasts, mitochondria, and peroxisomes during leaf senescence of nodulated pea plants. | in this work the influence of the nodulation of pea (pisum sativum l.) plants on the oxidative metabolism of different leaf organelles from young and senescent plants was studied. chloroplasts, mitochondria, and peroxisomes were purified from leaves of nitrate-fed and rhizobium leguminosarum-nodulated pea plants at two developmental stages (young and senescent plants). in these cell organelles, the activity of the ascorbate-glutathione cycle enzymes ascorbate peroxidase (apx), monodehydroascorba ... | 2006 | 16698815 |
| reactive oxygen and nitrogen species and glutathione: key players in the legume-rhizobium symbiosis. | several reactive oxygen and nitrogen species (ros/rns) are continuously produced in plants as by-products of aerobic metabolism or in response to stresses. depending on the nature of the ros and rns, some of them are highly toxic and rapidly detoxified by various cellular enzymatic and non-enzymatic mechanisms. whereas plants have many mechanisms with which to combat increased ros/rns levels produced during stress conditions, under other circumstances plants appear to generate ros/rns as signall ... | 2006 | 16698817 |
| the role of phb metabolism in the symbiosis of rhizobia with legumes. | the carbon storage polymer poly-beta-hydroxybutyrate (phb) is a potential biodegradable alternative to plastics, which plays a key role in the cellular metabolism of many bacterial species. most species of rhizobia synthesize phb but not all species accumulate it during symbiosis with legumes; the reason for this remains unclear, although it was recently shown that a metabolic mutant of a nonaccumulating species retains the capacity to store phb in symbiosis. although the precise roles of phb me ... | 2006 | 16703322 |
| transformation of the entomopathogenic fungus paecilomyces fumosoroseus with agrobacterium tumefaciens. | to test the suitability of the agrobacterium tumefaciens-mediated transformation (amt) method with paecilomyces fumosoroseus, a fungal pathogen that causes diseases in a wide range of insects including whiteflies. | 2006 | 16706904 |
| identification of genistein-inducible and type iii-secreted proteins of bradyrhizobium japonicum. | flagellin is the bulk protein secreted by bradyrhizobium japonicum. for easier identification of minor protein fractions, the flagellin genes bll6865 and bll6866 were deleted. extracellular proteins of the corresponding mutant were purified and separated by 2d gel electrophoresis. several of the protein spots were detectable only after addition of genistein to the growth medium-genistein is an isoflavone secreted by soybean that activates the expression of genes encoding a type iii secretion sys ... | 2006 | 16707185 |
| constitutive expression exposes functional redundancy between the arabidopsis histone h2a gene hta1 and other h2a gene family members. | the arabidopsis thaliana histone h2a gene hta1 is essential for efficient transformation of arabidopsis roots by agrobacterium tumefaciens. disruption of this gene in the rat5 mutant results in decreased transformation. in arabidopsis, histone h2a proteins are encoded by a 13-member gene family. rna encoded by these genes accumulates to differing levels in roots and whole plants; hta1 transcripts accumulate to levels up to 1000-fold lower than do transcripts of other hta genes. we examined the e ... | 2006 | 16751347 |
| silencing the flavonoid pathway in medicago truncatula inhibits root nodule formation and prevents auxin transport regulation by rhizobia. | legumes form symbioses with rhizobia, which initiate the development of a new plant organ, the nodule. flavonoids have long been hypothesized to regulate nodule development through their action as auxin transport inhibitors, but genetic proof has been missing. to test this hypothesis, we used rna interference to silence chalcone synthase (chs), the enzyme that catalyzes the first committed step of the flavonoid pathway, in medicago truncatula. agrobacterium rhizogenes transformation was used to ... | 2006 | 16751348 |
| potential of a 16s rrna-based taxonomic microarray for analyzing the rhizosphere effects of maize on agrobacterium spp. and bacterial communities. | bacterial diversity is central to ecosystem sustainability and soil biological function, for which the role of roots is important. the high-throughput analysis potential of taxonomic microarray should match the breadth of bacterial diversity. here, the power of this technology was evidenced through methodological verifications and analysis of maize rhizosphere effect based on a 16s rrna-based microarray developed from the prototype of h. sanguin et al. (environ. microbiol. 8:289-307, 2006). the ... | 2006 | 16751545 |
| subterfuge and manipulation: type iii effector proteins of phytopathogenic bacteria. | diverse gram-negative bacteria deliver effector proteins into the cells of their eukaryotic hosts using the type iii secretion system. collectively, these type iii effector proteins function to optimize the host cell environment for bacterial growth. type iii effector proteins are essential for the virulence of pseudomonas syringae, xanthomonas spp., ralstonia solanacearum and erwinia species. type iii secretion systems are also found in nonpathogenic pseudomonads and in species of symbiotic nit ... | 2006 | 16753033 |
| the regulatory function of the upstream sequence of the beta-conglycinin alpha subunit gene in seed-specific transcription is associated with the presence of the ry sequence. | beta-conglycinin, a major component of seed-storage proteins in soybean, comprises three subunits: alpha, alpha', and beta. expression of these genes is spatially regulated in a stringent manner and occurs during seed development. to understand the mechanisms that control expression of the alpha subunit gene, we analyzed the nucleotide sequence of the 2.9-kb region upstream of the gene. the upstream sequence up to -1357 or a series of its 5'-deleted derivatives was fused to the beta-glucuronidas ... | 2006 | 16755137 |
| [transgenic wheat (triticum aestivum l.) with increased resistance to the storage pest obtained by agrobacterium tumefaciens--mediated]. | the transgenic wheat of improved resistance to the storage pest was production. we have introduced the cowpea trypsin inhibitor gene (cpti) into cultured embryonic callus cells of immature embryos of wheat elite line by agrobacterium-mediated method. independent plantlets were obtained from the kanamycin-resistant calli after screening. pcr and real time pcr analysis, pcr-southern and southern blot hybridization indicated that there were 3 transgenic plants viz. transformed- i, ii and iii (t- i, ... | 2006 | 16755923 |
| [induction of cucumber hairy roots and effect of cytokinin 6-ba on its growth and morphology]. | hairy roots of cucumis sativus l. could be incited directly from the cut edges of 10-day-old cotyledon explants after infection with the strain agrobacterium rhizogenes atcc15834 harboring agropine-type plasmid, pria4b for 5 days. it was observed that the percentage of rooting cotyledon explants was more than 90 % 10 days after infection. hairy roots could grow rapidly and highly branched on solid plant growth regulator-free ms medium. the pcr amplification of rol b genes and vir c gene showed t ... | 2006 | 16755937 |
| characterization of 'schizokinen'; a dihydroxamate-type siderophore produced by rhizobium leguminosarum iari 917. | the rhizobia comprise one of the most important groups of beneficial bacteria, which form nodules on the roots (rarely on the stems) of leguminous plants. they live within the nodules and reduce atmospheric nitrogen to ammonia, which is further assimilated by plants into required nitrogenous compounds. the rhizobia in return obtain nutrition from the plant. rhizobia are free-living soil bacteria and have to compete with other microorganisms for the limited available iron in the rhizosphere. in o ... | 2006 | 16758117 |
| biosynthesis and bioactivity of trichosanthin in cultured crown gall tissues of trichosanthes kirilowii maximowicz. | trichosanthin (tcs) from trichosanthes kirilowii maximowicz (t. kirilowii) can be used to treat choriocarcinoma. in this work, we established a novel system to produce tcs in crown gall tissues of t. kirilowii infected by agrobacterium tumefaciens c58 (a. tumefaciens). in the crown gall tissues, a nopaline synthase (nos) gene of a. tumefaciens was identified by polymerase chain reaction (pcr), and nopaline accumulation was confirmed by a high-voltage filter paper electrophoresis. furthermore, we ... | 2006 | 16758196 |
| roles for redox regulation in leaf senescence of pea plants grown on different sources of nitrogen nutrition. | leaf senescence and associated changes in redox components were monitored in commercial pea (pisum sativum l. cv. phoenix) plants grown under different nitrogen regimes for 12 weeks until both nodules and leaves had fully senesced. one group of plants was inoculated with rhizobium leguminosarum and grown with nutrient solution without nitrogen. a second group was not inoculated and these were grown on complete nutrient solution containing nitrogen. leaf senescence was evident at 11 weeks in both ... | 2006 | 16760420 |
| [soybean lectin as a component of a composite biopreparation involving bradyrhizobium japonicum 634b]. | the effects of the composite biopreparation bralec (involving the soybean-specific root nodule bacterium bradyrhizobium japonicum strain 634b and soybean lectin at concentrations of 500, 50, and 5 microg/ml as major components) on the development and functional activity of soybean-rhizobium symbiosis (development phases of one leaf, four true leaves, and budding) was studied. it was demonstrated that pretreatment of seed with this preparation stimulated the development of both the macro- and mic ... | 2006 | 16761578 |
| the poor growth of rhodospirillum rubrum mutants lacking pii proteins is due to an excess of glutamine synthetase activity. | the p(ii) family of proteins is found in all three domains of life and serves as a central regulator of the function of proteins involved in nitrogen metabolism, reflecting the nitrogen and carbon balance in the cell. the genetic elimination of the genes encoding these proteins typically leads to severe growth problems, but the basis of this effect has been unknown except with escherichia coli. we have analysed a number of the suppressor mutations that correct such growth problems in rhodospiril ... | 2006 | 16762025 |
| 14-3-3 mediates transcriptional regulation by modulating nucleocytoplasmic shuttling of tobacco dna-binding protein phosphatase-1. | tobacco dbp1 is the founding member of a novel class of plant transcription factors featuring sequence-specific dna binding and protein phosphatase activity. to understand the mechanisms underlying the function of this family of transcriptional regulators, we have identified the tobacco 14-3-3 isoform g as the first protein interacting with a dbp factor. 14-3-3 recognition involves the n-terminal region of dbp1, which also supports the dna binding activity attributed to dbp1. the relevance of th ... | 2006 | 16762921 |
| glyphosate-induced anther indehiscence in cotton is partially temperature dependent and involves cytoskeleton and secondary wall modifications and auxin accumulation. | yield reduction caused by late application of glyphosate to glyphosate-resistant cotton (gossypium hirsutum; grc) expressing cp4 5-enol-pyruvylshikmate-3-p synthase under the cauliflower mosaic virus-35s promoter has been attributed to male sterility. this study was aimed to elucidate the factors and mechanisms involved in this phenomenon. western and tissue-print blots demonstrated a reduced expression of the transgene in anthers of grc compared to ovules of the same plants. glyphosate applicat ... | 2006 | 16766672 |
| the use of the phosphomannose-isomerase/mannose selection system to recover transgenic apple plants. | a selection system based on the phosphomannose-isomerase gene (pmi) as a selectable marker and mannose as the selective agent was evaluated for the transformation of apple (malus domestica borkh.). mannose is an unusable carbon source for many plant species. after uptake, mannose is phosphorylated by endogenous hexokinases to mannose-6-phosphate. the accumulation of mannose-6-phosphate leads to a block in glycolysis by inhibition of phosphoglucose-isomerase, resulting in severe growth inhibition ... | 2006 | 16770626 |
| structural characterization of a k-antigen capsular polysaccharide essential for normal symbiotic infection in rhizobium sp. ngr234: deletion of the rkpmno locus prevents synthesis of 5,7-diacetamido-3,5,7,9-tetradeoxy-non-2-ulosonic acid. | many early molecular events in symbiotic infection have been documented, although factors enabling rhizobium to progress within the plant-derived infection thread and ultimately survive within the intracellular symbiosome compartment as mature nitrogen-fixing bacteroids are poorly understood. rhizobial surface polysaccharides (sps), including the capsular polysaccharides (k-antigens), exist in close proximity to plant-derived membranes throughout the infection process. spss are essential for bac ... | 2006 | 16772294 |
| transgenic wheat progeny resistant to powdery mildew generated by agrobacterium inoculum to the basal portion of wheat seedling. | to improve the transformation efficiency of wheat (triticum aestivum l.) mediated by agrobacterium tumefaciens, we explored the possibility of employing the basal portion of wheat seedling (shoot apical meristem) as the explants. three genotypes of wheat were transformed by a. tumefaciens carrying beta-1, 3-glucanase gene. after vernalization, the seeds to be transformed were germinated. when these seedlings grew up to 2 approximately 5 cm, their coleoptile and half of the cotyledon were cut out ... | 2006 | 16773333 |
| [raising fat content in transgenic rice by anti-pep gene transformation]. | gene for phosphoenolpyruvate carboxylase (pepcase) was incorporated in antisense orientation into genome of japonica rice variety xiushui 11 through agrobacterium tumefaciens-mediated transformation of mature seeds-derived embryogenic calli, and a total of 95 transgenic plants were regenerated, which were confirmed by histochemical detection of gus activity (fig.2), pcr assay (fig.3) and southern hybridization analysis (fig.4). the transgene was inherited by the t1 progeny and mendelian 3:1 segr ... | 2006 | 16775403 |
| expression of recombinant digitalis lanata ehrh. cardenolide 16'-o-glucohydrolase in cucumis sativus l. hairy roots. | the coding sequence for the digitalis lanata ehrh. cardenolide 16'-o-glucohydrolase was inserted downstream of the 35s promoter in the binary vector pbi121 resulting in plant expression vector pbi121cgh. cotyledon explants excised from 10-day-old seedlings of cucumis sativus l. were transformed using agrobacterium rhizogenes 15834 harbouring pbi121cgh. hairy roots were obtained from infected cotyledon explants in vitro 10 days after inoculation. pcr amplification of coding sequences for cgh i, r ... | 2006 | 16775721 |
| effect of heat and ph stress in the growth of chickpea mesorhizobia. | the development of rhizobial inoculants requires the selection of isolates that are symbiotically efficient as well as adapted to the local environmental conditions. our aim was to find indigenous chickpea rhizobia tolerant to adverse environmental conditions, such as temperature and ph. thirteen isolates of chickpea mesorhizobia from southern portugal were examined. tolerance to stress temperatures and ph was evaluated by quantification of bacterial growth at 20-37 degrees c and ph 5-9, respect ... | 2006 | 16775779 |
| the arabidopsis thaliana transcriptome in response to agrobacterium tumefaciens. | the pathogen agrobacterium tumefaciens infects a broad range of plants, introducing the t-dna into their genome. contrary to all known bacterial phytopathogens, a. tumefaciens lacks the hypersensitive response-inducing hrp genes, although it introduces numerous proteins into the plant cell through a type iv secretion system. to understand the timing and extent of the plant transcriptional response to this unusual pathogen, we used an arabidopsis 26,000-gene oligonucleotide microarray. we inocula ... | 2006 | 16776300 |
| a microarray-based detection system for genetically modified (gm) food ingredients. | a multiplex dna microarray chip was developed for simultaneous identification of nine genetically modified organisms (gmos), five plant species and three gmo screening elements, i.e. the 35s promoter, the nos terminator and the nptii gene. the chips also include several controls, such as that for the possible presence of camv. the on-chip detection was performed directly with pcr amplified products. particular emphasis was placed on the reduction of the number of pcr reactions required and on th ... | 2006 | 16786296 |
| sequence analysis of the 144-kilobase accessory plasmid psmesm11a, isolated from a dominant sinorhizobium meliloti strain identified during a long-term field release experiment. | the genome of sinorhizobium meliloti type strain rm1021 consists of three replicons: the chromosome and two megaplasmids, psyma and psymb. additionally, many indigenous s. meliloti strains possess one or more smaller plasmids, which represent the accessory genome of this species. here we describe the complete nucleotide sequence of an accessory plasmid, designated psmesm11a, that was isolated from a dominant indigenous s. meliloti subpopulation in the context of a long-term field release experim ... | 2006 | 16672515 |
| flavonoid-inducible modifications to rhamnan o antigens are necessary for rhizobium sp. strain ngr234-legume symbioses. | rhizobium sp. strain ngr234 produces a flavonoid-inducible rhamnose-rich lipopolysaccharide (lps) that is important for the nodulation of legumes. many of the genes encoding the rhamnan part of the molecule lie between 87 degrees and 110 degrees of pngr234a, the symbiotic plasmid of ngr234. computational methods suggest that 5 of the 12 open reading frames (orfs) within this arc are involved in synthesis (and subsequent polymerization) of l-rhamnose. two others probably play roles in the transpo ... | 2006 | 16672619 |
| agrobacterium-mediated transformation of petunia leaf discs. | many dicotyledonous and also several monocotyledonous plant species are susceptible to agrobacterium-mediated transformation. this current and well-established method has been used successfully with a large number of plant species to mediate gene transfer. this chapter describes an agrobacterium-mediated transformation method of petunia hybrida leaf discs, one of the first species that was routinely transformed using this method. | 2006 | 16673922 |
| transformation of maize via agrobacterium tumefaciens using a binary co-integrate vector system. | this chapter describes a stepwise protocol to achieve success in genetic transformation of maize using agrobacterium tumefaciens as a dna delivery system. researchers will be able to effectively transform immature embryos of hi-ii and related genotypes with this protocol. the outcome of the transformation process will be transgenic embryogenic callus tissue, transgenic plants, and transgenic progeny seeds. recommendations for molecular confirmation and evaluation of transgenic tissue/plants are ... | 2006 | 16673926 |
| methods for regeneration and transformation in eschscholzia californica: a model plant to investigate alkaloid biosynthesis. | eschscholzia californica cham. (california poppy) is a plant species that accumulates pharmacologically active alkaloids biosynthetically related to the morphinan alkaloids of papaver somniferum. this, in combination with the relative ease with which it is propagated in vitro, makes it a key model for benzylisoquinoline biosynthesis. transformation techniques are an important tool for these studies and for metabolic engineering attempts. agrobacterium mediated transformation techniques for this ... | 2006 | 16673930 |
| the rhizobium leguminosarum biovar trifolii anu794 induces novel developmental responses on the subterranean clover cultivar woogenellup. | the clover-nodulating rhizobium leguminosarum bv. trifolii anu794 initiates normal root-nodule development with abnormally low efficiency on the trifolium subterraneum cv. woogenellup. the cellular and developmental responses of woogenellup roots to the site- and dose-defined inoculation of green fluorescent protein (gfp)-labeled cells of anu843 (nodulation proficient) and anu794 was investigated using light, fluorescence, and confocal microscopy. strain anu794-gfp induced three primordia types ... | 2006 | 16673934 |
| biological significance and development of practical synthesis of biotin. | biotin (1), a water-soluble b series vitamin, distributes widely in microorganisms, plants, and animals. biosynthesis of 1 involves five steps sequence starting from pimelic acid. the last step, a transformation from dethiobiotin (dtb) to 1, includes an iron clusters-mediated radical process. the compound 1 is a cofactor of carboxylation enzymes and plays crucial roles in the metabolism of fatty acids, sugars, and alpha-amino acids. in addition to the increasing application to feed additives, re ... | 2006 | 16676358 |
| pigment shuffling in antenna systems achieved by expressing prokaryotic chlorophyllide a oxygenase in arabidopsis. | the organization of pigment molecules in photosystems is strictly determined. the peripheral antennae have both chlorophyll a and b, but the core antennae consist of only chlorophyll a in green plants. furthermore, according to the recent model obtained from the crystal structure of light-harvesting chlorophyll a/b-protein complexes ii (lhcii), individual chlorophyll-binding sites are occupied by either chlorophyll a or chlorophyll b. in this study, we succeeded in altering these pigment organiz ... | 2006 | 16574646 |
| transgenic medicago truncatula plants obtained from agrobacterium tumefaciens -transformed roots and agrobacterium rhizogenes-transformed hairy roots. | medicago truncatula, barrel medic, is a forage crop that has been developed into a model legume. the development of new transformation methods is important for functional genomic studies in this species. based on agrobacterium tumefaciens-mediated transformation of root explants, we developed an effective system for producing m. truncatula (genotype r108) transgenic plants. among the four a. tumefaciens strains (agl1, c58c1, eha105 and lba4404) tested, eha105 and agl1 were most effective in rege ... | 2006 | 16575594 |
| [analysis of dna homology and 16s rdna sequence of rhizobia, a new phenotypic subgroup, isolated from xizang autonomous region of china]. | based on the studies of numerical taxonomy, the seven rhizobial strains isolated from the root nodules of leguminous plants trigonella spp. and astragalus spp. growing in the xizang autonomous region of china constituted a new phenotypic subgroup, where wide phenotypic and genotypic diversity among legume crops had been reported due to complex terrain and various climate. the new phenotypic subgroup were further identified to clarify its taxonomic position by dna homology analysis and 16s rdna g ... | 2006 | 16579480 |
| identification of genomic islands in six plant pathogens. | genomic islands (gis) play important roles in microbial evolution, which are acquired by horizontal gene transfer. in this paper, the gis of six completely sequenced plant pathogens are identified using a windowless method based on z curve representation of dna sequences. consequently, four, eight, four, one, two and four gis are recognized with the length greater than 20-kb in plant pathogens agrobacterium tumefaciens str. c58, rolstonia solanacearum gmi1000, xanthomonas axonopodis pv. citri st ... | 2006 | 16581205 |
| optimization of transient agrobacterium-mediated gene expression system in leaves of nicotiana benthamiana. | here we report on a simple and reproducible system of agrobacterium-mediated transient gene expression assay that utilizes infiltration of young nicotiana benthamiana leaves. although some of the phenomena described in this paper have been already reported by other researchers, here we have further developed them. the highest level of transient gfp gene expression was detected in the youngest leaves of n. benthamiana infiltrated with a. tumefaciens strains agl0 and eha105 precultured in the pres ... | 2006 | 16582986 |
| rhizobium-mediated induction of phenolics and plant growth promotion in rice (oryza sativa l.). | qualitative and quantitative estimation of phenolic compounds was done through reverse phase-high performance liquid chromatography (rp-hplc) from different parts (leaf, stem, and root) of rice plants after inoculation with two rhizobial strains, rre6 (rhizobium leguminosarum bv. phaseoli) and anu 843 (r. leguminosarum bv. trifolii) and infection by rhizoctonia solani. on the basis of their retention time, the major phenolic acids detected in hplc analysis were gallic, tannic, ferulic, and cinna ... | 2006 | 16586021 |
| isolation and identification of natural endophytic rhizobia from rice (oryza sativa l.) through rdna pcr-rflp and sequence analysis. | three novel endophytic rhizobial strains (rre3, rre5, and rre6) were isolated from naturally growing surface-sterilized rice roots. these isolates had the ability to nodulate common bean (phaseolus vulgaris). polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) and sequencing of 16s rdna of these isolates revealed that rre3 and rre5 are phylogenetically very close to burkholderia cepacia complex, whereas rre6 has affinity with rhizobium leguminosarum bv. phaseoli. plant ... | 2006 | 16586025 |
| thymol derivatives from hairy roots of arnica montana. | five known thymol derivatives were isolated from roots of arnica montana transformed with agrobacterium rhizogenes lba 9402. the compounds were characterized by spectral methods. the pattern of thymol derivatives in light-grown hairy roots was slightly different from that in dark-grown ones. this is the first report on the presence of thymol derivatives in hairy roots of the plant. | 2006 | 16586074 |
| azorhizobium doebereinerae sp. nov. microsymbiont of sesbania virgata (caz.) pers. | thirty-four rhizobium strains were isolated from root nodules of the fast-growing woody native species sesbania virgata in different regions of southeast brazil (minas gerais and rio de janeiro states). these isolates had cultural characteristics on yma quite similar to azorhizobium caulinodans (alkalinization, scant extracellular polysaccharide production, fast or intermediate growth rate). they exhibited a high similarity of phenotypic and genotypic characteristics among themselves and to a le ... | 2006 | 16564956 |
| rhizotoxicity of cadmium and copper in soil extracts. | dissolved organic matter (dom) influences metal speciation in soil solutions and, hence, metal toxicity. root-elongation experiments were conducted to examine the effect of soil solution components, such as ca, h, and dom, on metal rhizotoxicity. a biotic ligand model (blm) was tested for its ability to predict the rhizotoxicity of cd and cu in soil extracts. it was hypothesized that the concentration of cd and cu bound to functional groups at the root surface estimated using a blm would be a be ... | 2006 | 16566153 |
| the production of species-specific highly unsaturated fatty acyl-containing lcos from rhizobium leguminosarum bv. trifolii is stringently regulated by nodd and involves the nodrl genes. | a proportion of the nod factors of some rhizobium leguminosarum bv. trifolii strains is characterized by the presence of highly unsaturated fatty acyl chains containing trans double bonds in conjugation with the carbonyl group of the glycan oligosaccharide backbone. these fatty acyl chains are c18:3, c20:3, c18:4, or c20:4 and have uv-absorption maxima at 303 and 330 nm. these nod factors are presumed to be important for host-specific nodulation on clover species. however, in wild-type r. legumi ... | 2006 | 16570652 |
| [hairy root induction and plant regeneration of crownvetch (coronilla varia l.) transformed by agrobacterium rhizogenes]. | an efficient system of genetic transformation and plant regeneration via somatic embryogenesis was established in crownvetch (coronilla varia l.) by infecting the segments of cotyledons and hypocotyls of 15d-old seedlings with agrobacterium rhizogenes strain 15834. hairy roots were produced directly from the wounded surface of the explants or via calluses on hormone-free murashige and skoog (ms) medium after infection by a. rhizogenes. transformed roots grew rapidly either on solid or liquid ms ... | 2006 | 16572849 |
| [studies on chemical constituents of hairy root of cassia obtusifolia]. | to study the chemical constituents in hairy roots of cassia obtusifolia. | 2006 | 16573002 |
| a colorimetric method for the assay of adp-glucose pyrophosphorylase. | 2006 | 16574055 | |
| efficient production of agrobacterium rhizogenes-transformed roots and composite plants for studying gene expression in coffee roots. | the possibility of rapid validation and functional analysis of nematode resistance genes is a common objective for numerous species and particularly for woody species. in this aim, we developed an agrobacterium rhizogenes-mediated transformation protocol for coffea arabica enabling efficient and rapid regeneration of transformed roots from the hypocotyls of germinated zygotic embryos, and the subsequent production of composite plants. the a. rhizogenes strain a4rs proved to be the most virulent. ... | 2006 | 16596429 |
| sequencing and heterologous expression of an epimerase and two lyases from iminodisuccinate-degrading bacteria. | recently, degradation of all existing epimers of the complexing agent iminodisuccinate (ids) in the bacterial strain agrobacterium tumefaciens by6 was proven to depend on an epimerase and a c-n lyase (cokesa et al., appl. environ. microbiol. 70:3941-3947, 2004). in the bacterial strain ralstonia sp. strain slrs7, a corresponding c-n lyase is responsible for the initial degradation step (cokesa et al., biodegradation 15:229-239, 2004). the ite gene, encoding the ids-transforming epimerase, and th ... | 2006 | 16597988 |
| diverse bacteria associated with root nodules of spontaneous legumes in tunisia and first report for nifh-like gene within the genera microbacterium and starkeya. | we characterized 34 endophytic bacterial isolates associated to root nodules collected from spontaneous legumes in the arid zone of tunisia by 16s rdna polymerase chain reaction (pcr)-restriction fragment length polymorphism, whole cell protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), 16s rdna and 16s-23s rdna internal transcribed spacer sequencing. phylogenetically, these isolates belong to the branches containing the genera inquilinus, bosea, rhodopseudomonas, para ... | 2006 | 16598639 |