Publications

deficient phytohemagglutinin-induced interleukin-2 activity in patients with inactive systemic lupus erythematosus is correctable by the addition of phorbol myristate acetate.	in systemic lupus erythematosus (sle) patients, the production of interleukin-2 (il-2) by blood t lymphocytes in response to stimulation with phytohemagglutinin (pha) either alone or with phorbol myristate acetate (pma) or ionomycin, a ca2+ ionophore, was examined. deficiency in pha-stimulated il-2 production by cells from sle patients was repaired by the addition of pma, but not ionomycin. pma alone did not stimulate il-2 production but, in concert with pha, induced il-2 synthesis. moreover, pm ...	1988	3260781
lymphocyte subsets in peripheral blood and pleural fluid.	we have examined the distribution of b and t lymphocytes, t-cells with helper/inducer (t4+) or suppressor/cytotoxic (t8+) phenotypes and a subset of cells with natural killer (nk) activity and positive for the leu 7 (hnk-1) surface antigen in peripheral blood and in lymphocyte-rich pleural effusions of patients with tuberculosis or malignancies (mesothelioma and lung cancer with pleural metastasis). in individual patients, the percentages of t lymphocytes were uniformly higher in pleural effusio ...	1988	3260873
immunoregulatory studies in patients with iga nephropathy.	numerous studies have evaluated t cell subsets and in vitro iga synthesis in patients with iga nephropathy. these reports have resulted in the hypothesis that defective regulation of iga synthesis is important in the pathogenesis of iga nephropathy. baseline immunologic measurements were performed in a clinically well characterized group of 19 pediatric and 13 adult (greater than or equal to 18 years) patients with no macrohematuria or intercurrent infection at the time of study. mean percentage ...	1988	3260958
hiv target cells: effect of their infection by hiv on the pathogenesis of aids.	pathogenesis of hiv infection and expression of retroviral proteins are gradually being elucidated. antibody to hiv is a marker of past or present viral infection. the virus can be isolated from cultured lymphocytes of seropositive but not seronegative patients. sero-epidemiological studies show that the majority of infected patients are asymptomatic carriers without biological sign of immune depression. some then show immune abnormalities such as a decrease of cd4 cells in the blood; some patie ...	1988	3260982
enhancement of gait restoration in spinal injured patients by functional electrical stimulation.	functional electrical stimulation (fes) was applied in 50 spinal cord injury patients in an effort to restore standing and walking. all patients in the program with lesions from t4-t12 were able to stand by means of fes. walking, utilizing a four-channel stimulator, was accomplished by 25 patients with lesions in the range from t4-t12. these 25 patients represent approximately 5% of all spinal cord injured patients treated in the authors' rehabilitation facility during this time period. after us ...	1988	3261218
development of a practical electrical stimulation system for restoring gait in the paralyzed patient.	a percutaneous functional neuromuscular stimulation (fns) system is being developed for restoring function in the lower extremities of spinal cord injured people with lesions between t4 and t11. limitations of the current system are electrode failure, muscle fatigue, cumbersome electrical hardware, and an inefficient user-machine interface. transforming this experimental system into a practical fns system requires achieving acceptable levels of safety, reliability, function, metabolic energy req ...	1988	3261221
tumour infiltrating lymphocytes in nasopharyngeal carcinoma.	tumour infiltrating lymphocytes were studied in 28 cases of undifferentiated carcinoma using a panel of monoclonal antibodies. for the t subsets we used okt4 (helper/inducer), 8 (suppressor/cytotoxic), 11 (pan t), 9 (transferrin receptor) and dako t6. for the b cells we used polyclonals igm, d, g and a. others were hnk-1 (natural-killer cells), thb-5 and w6/32 (hla-abc) and hla-dr. most of the lymphocytes were t cells and in 26 of 28 biopsies there were more t8 than t4 cells which could be seen ...	1988	3261567
t4/t8 ratio and absolute t4 cell numbers in different clinical stages of kaposi's sarcoma in aids.	thirty-seven men (36 homosexual or bisexual and one heterosexual) with epidemic kaposi's sarcoma and underlying hiv infection were followed up over a period of up to 32 months. fourteen patients (38%) died, with a median survival time of 7.2 months after the diagnosis of aids. seventeen patients (46%) presented with one or more opportunistic infections, mostly pneumocystis carinii pneumonia. eighteen patients (49%) had lymphadenopathy syndrome according to the definition of the cdc. using the la ...	1988	3261596
progressive cellular immune impairment leading to development of aids: two-year prospective study of hiv infection in drug addicts.	we have studied immunological, serological and clinical abnormalities in 264 hiv-positive and hiv-negative drug abusers. ninety percent of the 264 drug addicts (mean age 26 +/- 0.8 years) were found to have hiv antibodies and there was a significant increase (p less than 0.01) in the frequency of hiv antibody positivity with increasing duration of exposure to parenteral drug abuse. there was a very strong correlation between the progressive decline of the mean t4+ helper/inducer cells and t4+/t8 ...	1988	3261660
phenotypes and spontaneous cell cytotoxicity of mononuclear cells from patients with seronegative spondyloarthropathies: ankylosing spondylitis, psoriatic arthropathy and pauciarticular juvenile chronic arthritis--analysis of mononuclear cells from peripheral blood, synovial fluid and synovial membranes.	t-cell subpopulations and natural killer (nk) cells from peripheral blood, synovial fluid and synovial membranes from patients with seronegative spondyloarthropathies were investigated. thirty-four patients with ankylosing spondylitis, sixteen patients with psoriatic arthropathy and six patients with pauciarticular juvenile chronic arthritis were studied. all the patient groups had normal proportions of t4+ and t8+ cells as well as normal t4/t8 ratios in peripheral blood. in the synovial fluids ...	1988	3261676
binding site for human immunodeficiency virus coat protein gp120 is located in the nh2-terminal region of t4 (cd4) and requires the intact variable-region-like domain.	the external segment of the t4 (cd4) glycoprotein functions as the t-cell surface receptor for human immunodeficiency virus by binding the major viral coat protein (gp120) with relatively high affinity. to more precisely define the region of t4 involved in gp120 interaction, we used purified, soluble forms of t4 anchor-minus polypeptides (produced in a baculovirus system) in conjunction with proteolytic fragmentation, microsequencing, and a specific t4-gp120 binding assay. the results indicate t ...	1988	3261864
autogenous regulatory site on the bacteriophage t4 gene 32 messenger rna.	we have identified the binding site on the bacteriophage t4 gene 32 mrna responsible for autogenous translational regulation. we demonstrate that this site is largely unstructured and overlaps the initiation codon of gene 32 as previously predicted. co-operative binding of gene 32 protein to this site specifically blocks the formation of 30 s-trna(fmet)-gene 32 mrna ternary complexes and initiation of translation. the translational operator is bound co-operatively by gene 32 protein and this bin ...	1988	3262167
thermal denaturation of t4 gene 32 protein: effects of zinc removal and substitution.	gene 32 protein (g32p), the single-stranded (ss) dna binding protein from bacteriophage t4, is a zinc metalloprotein. the intrinsic zinc is one of the factors required for the protein to bind cooperatively to a ssdna lattice. we have used differential scanning calorimetry to determine how the thermodynamic parameters characterizing the denaturation of g32p are affected by removal or substitution of the intrinsic zinc. over a wide concentration range (1-10 mg/ml), the native zn(ii) protein unfold ...	1988	3262371
human recombinant interleukin-1 beta decreases plasma thyroid hormone and thyroid stimulating hormone levels in rats.	thyroid function was investigated in rats treated sc with a single injection of human recombinant interleukin-1 beta (hril-1). in 5 h 12.5 micrograms hril-1 decreased total serum t4 levels by 30 +/- 2% (p less than 0.01) and serum t3 levels by 35 +/- 4% (p less than 0.001). however free t4 and t3 fractions increased markedly within the first 140 min by 162 +/- 20% (p less than 0.001) and by 55 +/- 4% (p less than 0.001) resulting in a 88 +/- 20% increase in the free t4 concentration (p less than ...	1988	3262503
distribution of t cell subsets in follicular fluid.	examination of follicular fluid (ff) from in vitro fertilization patients revealed a significant difference in concentrations of lymphocytes and t cell subpopulations with increased oocyte maturation. a total of 111 follicles containing 82 oocytes were aspirated from 10 patients undergoing laparoscopic oocyte retrieval. ff from 61.3% of the follicles was classified as clear and 38.7% as bloody, based on gross and microscopic appearance. a mean of 1.78 x 10(6) lymphocytes/ml was obtained from per ...	1988	3262539
serum thymulin in human zinc deficiency.	the activity of thymulin (a thymic hormone) is dependent on the presence of zinc in the molecule. we assayed serum thymulin activity in three models of mildly zinc-deficient (zd) human subjects before and after zinc supplementation: (a) two human volunteers in whom a specific and mild zinc deficiency was induced by dietary means; (b) six mildly zd adult sickle cell anemia (sca) subjects; and (c) six mildly zd adult non-sca subjects. their plasma zinc levels were normal and they showed no overt c ...	1988	3262625
flavonoids specifically inhibit iodothyronine-deiodinase in rat hepatocytes.	functionally intact hepatocytes in suspension metabolize iodothyronines (t4, rt3) by 5'-deiodination. these ith-5h-deiodination reactions are inhibited by flavonoids. the structure-activity relationships are similar to those found for the 5'-d reaction using microsomal membranes as enzyme source. different effects of flavonoids on the 5'-deiodination reaction of t4 and rt3 point to additional effects, e.g. ith cellular uptake, transport and binding. estimation of gluconeogenesis from lactate by ...	1988	3262876
immune cells and the hypothalamic-pituitary axis in major depression.	to explore changes in immune cell status with changes in the hypothalamic-pituitary (hp) axis in 20 patients with major depression as compared with 20 age-, sex-, and race-matched control subjects, we examined peripheral blood mononuclear cells (pbmc) for total t-cells (t3), total b-cells (b1), two t-cell subsets (t4 and t8), and natural killer cells (nkh1), and we measured the plasma level of cortisol, adrenocorticotropic hormone (acth), growth hormone (gh), and prolactin (prl). the ratio of t4 ...	1988	3262880
aids as immune system activation: a model for pathogenesis.	epidemiological evidence has implicated the human immunodeficiency virus (hiv) as the etiological agent of the acquired immunodeficiency syndrome (aids) (barré-sinoussi et al., 1983; sarngadharan et al., 1984; kitchen et al., 1984; levy et al., 1984). primary infection with hiv is accompanied by an acute flu-like illness followed by a relatively long period of asymptomatic infection, the delayed appearance of lymphadenopathy and a progressive decline in immune responsiveness. eventually, signifi ...	1988	3263225
increase in antideoxyribonucleic acid antibody titer in postpartum aggravation of autoimmune thyroid disease.	serum antidouble stranded dna antibody levels were measured during pregnancy and after delivery in women who had postpartum exacerbations of graves' and hashimoto's diseases. the changes in serum anti-dna antibody levels closely paralleled those in the serum free t4 index, significantly increasing in the thyrotoxic phase 3-8 months postpartum in women with postpartum exacerbations of thyrotoxicosis due to graves' disease and 1-3 months postpartum in women with postpartum destructive thyrotoxicos ...	1988	3263380
effect of a light pulse during the dark on photoperiodic regulation of the rate of thyroxine-induced, spontaneous, and prolactin-inhibited metamorphosis in rana pipiens tadpoles.	since rana pipiens tadpoles injected with thyroxine (t4) early in the dark develop more slowly than those injected in the light, we studied the effect of giving a light pulse of 1 hr early in the dark. tadpoles injected under a 7.5-w red light bulb in a darkened room with 0.2 microgram t4 daily at 2200 hr went through metamorphosis faster on a 12l:3d:1l:8d cycle with a light pulse after injection than on a 12l:12d cycle without a light pulse, and even faster on a 12l:1.5d:1l:9.5d cycle with a li ...	1988	3263470
cellular regulation of anti-nrnp antibody synthesis is different from that of anti-dna antibody synthesis in patients with systemic lupus erythematosus.	the cellular regulation of anti-nuclear ribonucleoprotein (nrnp) antibody synthesis in patients with systemic lupus erythematosus (sle) was examined and compared with that of anti-double-stranded dna (dsdna) antibodies. in vitro antibody production by lymphocytes from sle patients with antibodies to either dsdna or nrnp alone was measured using dsdna-specific and nrnp-specific solid-phase radioimmunoassays (ria). lymphocytes of sle patients with only anti-dsdna antibodies and normal individuals ...	1988	3263692
a prospective study of hiv-infected haemophilic boys and the prognostic significance of immune and haematological abnormalities.	fifty-seven haemophilic boys have been studied prospectively determining t lymphocyte subsets and haematological parameters over a period of 26 months. clinical follow up of these patients has continued for a further 41 months. at the beginning of the study 21 boys were hiv antibody seropositive and seroconversion was documented in a further 9 during the study period, giving a 52.6% incidence of hiv seropositivity. it has been possible to define a group of patients with persistently falling t4 v ...	1988	3263713
t-cell subsets in the cerebrospinal fluid and blood of patients with multiple sclerosis.	the absolute numbers and ratios of helper/inducer (t4) and cytotoxic/suppressor (t8) t-cells were determined in cerebrospinal fluid (csf) and blood of patients with multiple sclerosis (ms) and various other neurologic diseases (ond). in patients with ms, the t4:t8 ratio was higher in both blood and csf, and the increase was significantly greater in csf than in blood. these findings were due to an increased proportion of t4-lymphocytes in the csf and to a decreased proportion of t8-cells in blood ...	1988	3264287
characterization of gp120 binding to cd4 and an assay that measures ability of sera to inhibit this binding.	there is evidence that the initial interaction between hiv-1 and the host that is essential for infection is the specific binding of the viral envelope glycoprotein, gp120, to the cd4 molecule found on certain t cells and monocytes. most individuals infected with hiv develop antibodies against the gp120 protein. although in vitro treatment of cd4+ t cells with mab to a specific epitope of the cd4 molecule (t4a) blocks virus binding, syncytia formation, and infectivity, it is unclear if antibodie ...	1988	3264307
age, cellular immunity and the hp axis in major depression.	1. to assess the effect of age on cellular immune status and the hp axis in patients with major depression, we examined peripheral blood mononuclear cells (pbmc) and measured the plasma level of four neurohormones. 2. in 36 subjects, decreasing t lymphocyte response to con a covaried with age. percent of lymphocytes labeled as t8 lymphocytes tended to decrease and t4/t8 ratio tended to increase with increasing age. 3. hamilton and beck scores were significantly different between the two sex and ...	1988	3265523
methimazole treatment of 262 cats with hyperthyroidism.	the efficacy and safety of the antithyroid drug methimazole were evaluated over a 3-year period in 262 cats with hyperthyroidism. in 181 of the cats, methimazole was administered for 7 to 130 days (mean, 27.7 days) as a preoperative preparation for thyroidectomy. the remaining 81 cats were given methimazole for 30 to 1,000 days (mean, 228 days) as sole treatment for the hyperthyroid state. after 2 to 3 weeks of methimazole therapy (10 to 15 mg/d), the mean serum thyroxine (t4) concentration decr ...	1988	3265728
increased level of soluble interleukin-2 receptor in advanced solid tumors: a preliminary study.	both activated normal and transformed lymphocytes produce not only cell-associated but also cell-free il-2r. evidence of high serum concentrations of il-2r appears to serve as a tumor marker in patients with lymphomas on the contrary, the role of soluble il-2r in solid neoplasms has still to be defined. this investigation was carried out to analyze soluble il-2r production in human solid tumors. the study included 35 patients with solid tumors (12 without and 23 with metastases), 58 healthy subj ...	1988	3266042
[the effect of nucleotides on the sedimentation properties of contracted sheath of bacteriophage t4].		1988	3266140
human hybridoma suppressor factor (hsf) inhibits il2 production in addition to suppressing immunoglobulin production.	the human thymus cell hybridoma, 8e-24, secretes a potent immunosuppressive factor(s), hsf, which inhibits polyclonal immunoglobulin (ig) production. our current studies reveal that this suppression is monocyte dependent in that its suppressive activity for ig production was not observed in monocyte-depleted lymphocyte cultures but was restored by addition of monocytes. the requirement for monocytes was equally satisfied by autologous or allogeneic monocytes or monocyte conditioned media. althou ...	1988	3266185
[treatment using lymphocytapheresis of severe forms of crohn's disease. preliminary results].	we report our experience in the treatment of severe crohn's disease by lymphocytapheresis (lctph). twelve patients with severe crohn's disease unresponsive to prednisone for two years were treated by twelve sessions of lctph over a three-week period. approximately 4.5 x 10(9) lymphocytes were removed at the end of each session, total lymphocyte removal per patient ranging from 1.9 to 10.7 x 10(8)/kg. sessions were well tolerated. clinical improvement was observed in all patients at the end of th ...	1988	3266835
in vivo depression of lymphocyte traffic in sheep by vip and hiv (aids)-related peptides.	core pentapeptides and an octapeptide (peptide t) computer deduced from amino acid sequences from vasoactive intestinal peptide (vip) and the 120 gp external envelope of the hiv (aids) virus and synthesized have been reported to have important in vitro and in vivo activity including inhibition of hiv binding to cd4 surface antigens of brain cells and lymphocytes and limitation of hiv infectivity. two of these core pentapeptides, peptide ttnyt (peptide t [4-8]) and peptide tdnyt (vip [7-11]), are ...	1988	3267011
cell-mediated immunity in patients with sickle cell anaemia.	homozygous sickle cell disease patients have an increased risk of developing severe infections, probably because of impaired immunity. cellular immunity was studied in thirty-two patients with s homozygous haemoglobin (ss) and compared to 32 a homozygous haemoglobin (aa) healthy subjects. a leukocytosis was observed but with a significant diminution of t4 and t8 proportions in sickle cell patients. b lymphocytes, concanavalin a, phytohaemagglutinin, and phorbol myristate acetate-induced lymphocy ...	1988	3267159
computerized quality control of radioimmunoassay in korea.	automated data processing and quality control of radioimmunoassays offer not only increased speed but also a more thorough and statistically rigorous analysis of results. an external quality assessment scheme for serum thyroxine, triiodothyronine and thyroid stimulating hormone (tsh) assays was performed in five nuclear medicine laboratories in korea to compare with the assay performances of the world health organization radioimmunoassay program. the required radioimmunoassay kits were supplied ...	1988	3267360
type of feeding does not influence thyroid hormone levels in premature infants.	thirty premature neonates (mean gestational age 31.8 +/- 3.90 weeks; mean birth weight 1569 +/- 236 g) were divided into three groups receiving breast milk, robolact and pre-aptamil formula feeding. the infants with and without neonatal idiopathic respiratory distress syndrome (irds) were separately evaluated. no effect of the type of feeding and of a previously experienced irds on the blood t3-, t4-, and ths-levels could be demonstrated at the age of 6 weeks.	1988	3269262
phenotypic and molecular heterogeneity in philadelphia chromosome-positive acute leukemia.	philadelphia chromosome-positive (ph1) acute leukemia is a heterogeneous subset of acute leukemia with a poor prognosis. we studied five patients to determine the potential for phenotypic and molecular heterogeneity. cellular characterization studies included light myeloperoxidase (l-mpo), terminal deoxynucleotidyl transferase (tdt), ultrastructural mpo (u-mpo), and immunophenotyping by flow cytometry using t11, t3, t4, t8, leu 1, b1, leu 12, hla-dr (la), calla (j5), okm1, my4, my7, my8, my9, an ...	1988	3334895
thyroxine 5'-deiodinase activity in pineal gland and frontal cortex: nighttime increase and the effect of either continuous light exposure or superior cervical ganglionectomy.	type ii t4 5'-deiodinase (5'-d) activity was studied in pineal gland, frontal cortex, and anterior pituitary of male rats. enzymatic activity was determined by the release of 125i using [3',5'-125i]t4 as substrate. daytime levels of 5'-d activity were maximal for anterior pituitary (346.2 +/- 142.5 fmol 125i released/mg protein.h), followed by pineal gland (61.5 +/- 8.7 fmol 125i released/mg protein.h) and frontal cortex (3.1 +/- 0.5 fmol 125i released/mg protein.h). twenty-four-hour fluctuation ...	1988	3335206
localization of 7b2, neuromedin b, and neuromedin u in specific cell types of rat, mouse, and human pituitary, in rat hypothalamus, and in 30 human pituitary and extrapituitary tumors.	the distributions of three novel peptides, 7b2, neuromedin b, and neuromedin u, in rat, mouse, and human pituitaries, rat hypothalamus, and 30 human pituitary tumors were investigated with immunocytochemistry. immunoreactivity for 7b2 was present in rat, mouse, and human gonadotropes, in intermediate lobe cells and posterior lobe nerve fibers in rats and mice, in rat hypothalamus (particularly in the median eminence), and in eight human pituitary gonadotropinomas. in gonadectomized rats, larger, ...	1988	3335208
dna repair synthesis in human fibroblasts requires dna polymerase delta.	when uv-irradiated cultured diploid human fibroblasts were permeabilized with brij-58 then separated from soluble material by centrifugation, conservative dna repair synthesis could be restored by a soluble factor obtained from the supernatant of similarly treated hela cells. extensive purification of this factor yielded a 10.2 s, 220,000-dalton polypeptide with the dna polymerase and 3'- to 5'-exonuclease activities reported for dna polymerase delta ii (crute, j. j., wahl, a. f., and bambara, r ...	1988	3335506
late sigma factor of bacteriophage t4. formation and properties of rna polymerase-promoter complexes.	bacteriophage t4 late gene promoters do not display sequence homology in the -35 region (christensen, a. c., and young, e. t. (1982) nature 299, 369-371), suggesting an unusual geometry of rna polymerase-promoter interaction. we have analyzed in vitro utilization of a late t4 promoter by rna polymerase reconstituted from e. coli core enzyme (e) and bacteriophage t4 late sigma factor (sigma gp55). the e sigma gp55 holoenzyme forms a stable promoter complex which lacks protein-dna contacts upstrea ...	1988	3335538
enhanced thyroid iodine metabolism in patients with triiodothyronine-predominant graves' disease.	some patients with hyperthyroid graves' disease have increased serum t3 and normal or even low serum t4 levels during treatment with antithyroid drugs. these patients with elevated serum t3 to t4 ratios rarely have a remission of their hyperthyroidism. the aim of this study was to investigate thyroid iodine metabolism in such patients, whom we termed t3-predominant graves' disease. mean thyroid radioactive iodine uptake was 51.0 +/- 18.1% ( +/- sd) at 3 h, and it decreased to 38.9 +/- 20.1% at 2 ...	1988	3335601
impairment of prednisolone disposition in patients with graves' disease taking methimazole.	this study was undertaken to determine the effect of methimazole on the pharmacokinetics of iv prednisolone in patients with graves' disease. twenty women were studied, including eight with severe infiltrative ophthalmopathy who had taken methimazole and t4 for at least 4 months, six with severe infiltrative ophthalmopathy who had undergone subtotal thyroidectomy and, therefore, required no antithyroid treatment, and six age-matched normal women. all were euthyroid. each women received 0.54 mg/k ...	1988	3335606
lipoproteins, lipolytic enzymes, and hormonal status in hypothyroid women at different levels of substitution.	serum lipoproteins and postheparin plasma lipoprotein lipase and hepatic lipase (hl) activities were determined in 23 hypothyroid women treated with graded doses of thyroxine (t4) (50, 100, and 150 micrograms/day), each given for 3 weeks. since the sex hormone-binding globulin (shbg) and thereby serum sex steroid concentrations are sensitive to thyroid status, we also measured serum testosterone, estradiol, and shbg at each time. stepwise t4 treatment resulted in gradual improvement in thyroid s ...	1988	3335609
isolation of bacteriophage t4 baseplate proteins p7 and p8 and in vitro formation of the p10/p7/p8 assembly intermediate.	two bacteriophage t4 proteins, p7 and p8, which are components of the phage baseplate have been purified to apparent homogeneity. p7 and p8 are the protein products of t4 genes 7 and 8. a plasmid has been constructed which contains approximately 5 kilobases of t4 dna, including genes 7 and 8, under the control of the tac promoter. induction of escherichia coli w3110iq cells containing this plasmid resulted in the production of functional p7 and p8. standard protein isolation procedures were used ...	1988	3336066
treatment results for simultaneous primary squamous cell carcinomas of the head and neck.	forty patients with simultaneous multiple primaries of the head and neck were treated with curative intent between june 1964 and april 1983. the primary sites were treated with radiation therapy alone or in combination with surgery. surgery was used to salvage local failures of radiation treatment. neck disease was treated with radiation therapy alone or with neck dissection added after completion of radiation therapy to the primary lesions. minimum follow-up was 2 years with a range of 2 to 20 ...	1988	3336266
influence of hormones on glycogen and glucose metabolism in embryonic chick intestine.	previous studies on the development of embryonic intestine in vitro have revealed a stimulation of epithelial differentiation by the hormones hydrocortisone (hc) and thyroxine (t4). to determine whether these hormones also influence epithelial metabolism, duodena from 14-day-old chicken embryos were cultured for 72 h in the absence of hormones (controls) or in the presence of 1 nm t4 or 1 microm hc. in control cultures, glycogen accumulated within the duodenal epithelium to the level found at 17 ...	1988	3337234
interdigitating cell sarcoma. a morphologic, immunohistologic, and enzyme-histochemical study.	a 58-year-old man presented with an unusual sarcoma of the cervical lymph node. the tumor also involved the mesenteric lymph node and jejunum. tumor cells possessed intracytoplasmic s100 protein, leu-3a (t4), and hla-dr antigens. the neoplastic cells also showed membranous atpase activity. leum1, t6, leu1, leu2a, b1, lysozyme, and immunoglobulin were not recognized. their fine structure was similar to that of interdigitating cells. these data are consistent with derivation from lymph node interd ...	1988	3338024
effect of lot-to-lot variability in filter paper on the quantification of thyroxin, thyrotropin, and phenylalanine in dried-blood specimens.	we prepared whole-blood pools to contain various concentrations of phenylalanine (phe), thyroxin (t4), and thyrotropin (tsh) and applied them to six different lots of schleicher & schuell grade 903 filter paper, two of which represented extremes for serum-absorbancy. individual measured t4 values showed minimal overlap among all pools for each individual filter-paper lot and for all lots combined, but phe values overlapped considerably among the high-concentration pools within and among lots. in ...	1988	3338186
effects of upper dorsal sympathectomy on esophageal motility in humans.	to evaluate the role of the sympathetic nervous system in modulating esophageal motility, esophageal manometry was performed on two groups of patients who underwent upper dorsal sympathectomy for relief of palmar hyperhydrosis. in six patients sympathectomy was done by the supraclavicular approach, with removal of t2 and t3 ganglia. manometry was performed before the operation and three weeks after it. in seven other patients sympathectomy was done by the axillary approach, with removal of t2-t4 ...	1988	3338363
preferential saturation of brain 3,5,3'-triiodothyronine receptor during development in fetal lambs.	the concentrations of t4 and t3 were measured in brain, liver, and lung of fetal lambs at 100 days gestational age. the highest concentrations of t4 were found in lung (26.8 ng/g). brain t4 (8.8 ng/g) was only 30% of lung t4. in contrast, higher concentrations of t3 were found in brain (1.8 ng/g) than in lung (0.39 ng/g) or liver (0.36 ng/g). nuclear t3 was 16% of the total t3 in brain and 44% of that in lung. the degree of saturation of the nuclear t3 receptor was estimated from the concentrati ...	1988	3338407
different response to maternal hypothyroidism during the first and second half of gestation in the rat.	female rats were mated and thyroidectomized (t) on the same day and divided into four groups. three groups were subsequently treated daily with 1.8 micrograms l-t4/100 g body wt: 1) for the first 12 days [t + t4 (i)]; 2) from the 12th day until death [t + t4 (ii)]; or 3) for the entire 21-day study [t + t4 (i + ii)]. the other t animals were maintained without treatment. (t), and another group of mated rats were sham operated (c). maternal body weight increase during gestation did not differ bet ...	1988	3338409
altered triiodothyronine metabolism in zucker fatty rats.	genetically obese zucker fatty rats require two autosomal recessive genes (fa/fa) to express the obese phenotype. the obese zucker rat (fa/fa) has decreased total and free serum t3 concentrations, but normal serum t4 concentrations, compared to those in their lean littermates. to elucidate the mechanism of these differences, we measured the mcr and production rate (pr) of t4 and t3 in the three genotypes of 4-month-old male zucker rats (fa/fa, fa/fa, and fa/fa). in addition, 5'-deiodinase activi ...	1988	3338415
thyroid, parathyroid, and salivary gland evaluations in patients exposed to multiple fluoroscopic examinations during tuberculosis therapy: a pilot study.	the prevalence of thyroid, parathyroid, and salivary abnormalities was determined in 91 women who received an average of 112 fluoroscopic chest examinations during pneumothorax treatment for tuberculosis more than 40 yr previously and in 72 women treated for tuberculosis by other modalities. thyroid abnormalities were determined by physical examination, scintiscans, and measurements of serum free t4 index, tsh, and thyroid microsomal antibodies. thyroid nodules were diagnosed in 7.7% of the expo ...	1988	3339110
hyperthyroid chorea in an elderly woman associated with sole elevation of t3.	a 72-year-old woman presented with chorea, mild tachycardia, and elevation of t3ria. t4 was not elevated until the third determination. chorea resolved with treatment of hyperthyroidism. this case represents the first case of t3 hyperthyroid chorea and the oldest patient with hyperthyroid chorea described in the literature. it demonstrates yet another subtle way in which thyroid disease can present in the elderly.	1988	3339233
rat transthyretin: effects of acute short-term food deprivation and refeeding on serum and cerebrospinal fluid concentration and on hepatic mrna level.	to gain some insight into the nutritional factors that affect the blood level of transthyretin (ttr) and its metabolism, we have investigated the response of rat ttr to 1, 2 and 3 d of fasting and to 24 h of fasting followed by refeeding. the observed changes were compared to the level of ttr in cerebrospinal fluid (csf) and to the amount of circulating thyroid hormones. dot hybridization of a hepatic mrna-cdna probe specific for ttr was used to measure the relative level of ttr mrna. serum ttr ...	1988	3339478
the effect of small increases in dietary iodine on thyroid function in euthyroid subjects.	dietary iodine intake in the united states is greater than that considered necessary for the maintenance of normal thyroid function. the administration of pharmacologic quantities of iodine (10 to 1,000 mg daily) to euthyroid subjects results in small decreases in the serum t4 and t3 concentrations and a compensatory increase in the basal and trh-stimulated serum tsh concentrations. studies were carried out to determine whether a far smaller increase in iodine intake would also affect thyroid fu ...	1988	3340004
thyroid hormone levels and cigarette smoking in baboons.	using a primate animal model, two studies were undertaken to examine the effects of cigarette smoking on thyroid hormone levels. in study 1, mean total triiodothyronine (total t3) and mean total thyroxine (total t4) levels were measured in two groups of baboons (papio cynocephalus) who were taught to smoke cigarettes using operant conditioning techniques. the smokers were divided into established and naive smokers according to pack-years of exposure. a control group of never-smoker baboons was i ...	1988	3340630
phage t4 dna codes for two distinct 10-kda proteins which strongly bind to rna polymerase.	radioactive proteins have been synthesized in vitro from a coupled transcription-translation system primed with bacteriophage t4 dna. the labeled proteins were chromatographed on rna polymerase-sepharose affinity columns in order to identify a protein which comigrates with the 10-kda anti-sigma subunit of t4-modified rna polymerase. when we primed the in vitro system with specific restriction fragments of t4 dna, we found that there seemed to be two widely separated genes which code for this pro ...	1988	3341116
[current status of the treatment of urothelial tumors].	cancer of the urinary bladder, renal pelvis and ureter is usually transitional cell carcinoma. one third of cases of urethral cancer are also transitional cell carcinoma. in planning the treatment for these urothelial cancers, the anatomic stage (ta-t4), the histologic grade (1-3), tumor multiplicity and tumor size are generally taken into account. superficial and low-grade tumors can usually be treated by transurethral resection. however, such patients run the risk of subsequent tumor recurrenc ...	1988	3341782
characterization of a cytosolic triiodothyronine binding protein in atrium and ventricle of rat heart with different sensitivity toward thyroid hormone levels.	cytosolic t3-binding protein (ctbp) has been identified in both the ventricle and atrium of adult rat hearts. its biochemical characteristics and concentration have been determined in the two tissues as a function of thyroid hormone level. in both tissues association and dissociation constants were, respectively, k+1 = 1.3 x 10(8) m-1/min and k-1 = 0.025 min-1. scatchard analysis of t3 equilibrium binding data revealed a single class of binding sites (ka = 3.8 x 10(8) m-1). the maximal binding c ...	1988	3342743
increased plasma clearance rate of thyroxine despite decreased 5'-monodeiodination: study with a peroxisome proliferator in the rat.	in euthyroid rats a 17-day treatment with nafenopin, a hypolipidemic agent and peroxisome proliferator, decreased serum total and free t4 concentrations to 32 +/- 5% and 62 +/- 8% (mean +/- sem; n = 10), respectively, with no change in serum t3 and tsh concentrations. in methimazole-treated rats infused with 3 nmol t4/day/100 g bw, the nafenopin inhibitory effect was not significantly different from that in euthyroid rats. nafenopin treatment had the following effects on peripheral t4 and t3 met ...	1988	3342745
the effect of thyroid dysfunction and fasting on placenta inner ring deiodinase activity in the rat.	the placenta contains iodothyronine 5-deiodinase activity (p5-dase) that probably acts on iodothyronines in the fetal circulation to convert t4 to rt3 and t3 to 3,3'-t2. since thyroid status and fasting have profound effects on iodothyronine deiodinases in other tissues, the present studies were performed to determine if these perturbations affected p5-dase. control and treated rats were mated and killed near term on the 20th day of gestation. p5-dase was determined in placenta homogenates enric ...	1988	3342754
effects of thyrotropin on the vascular conductance of the thyroid gland.	it is well established that tsh from the anterior pituitary is the principal stimulatory agent in the physiological regulation of the thyroid gland. chronic elevations of plasma tsh induce hyperplasia and hypertrophy of thyroid follicular cells and enlargement of blood capillaries. at low plasma tsh levels the thyroid gland atrophies. we have examined the vascular conductance (c = blood flow/mean arterial pressure) of the thyroid gland and several other tissues over a wide range of endogenous pl ...	1988	3342758
nasopharyngeal carcinoma: result of treatment with cis-diamminedichloroplatinum ii, 5 fluorouracil, and radiation therapy.	combined ct (cddp + 5fu) and rt were given to 28 patients with npc during july 1982-may 1985. two, 1, 4, and 21 were in stages i-iv (ajc), respectively. none had distant metastasis. four did not complete the planned treatment, and one each had more or fewer ct courses than planned. the median duration of follow-up of surviving patients was 29 months (19-52,x 31.1). objective response (cr + pr) at the primary lesion was 27/28 (96.4%), whereas cr was 23/28 (82%). cr + pr and cr of the regional nod ...	1988	3343153
serial determination of thyroxine concentrations in hyperthyroid cats.	serum thyroxine (t4) concentrations of 10 hyperthyroid cats were measured at hourly intervals between 9 am and 4 pm. in 5 cats, blood samples were obtained by jugular venipuncture; the remaining 5 cats had blood samples obtained from jugular catheters. over the 7-hour period, a significant temporal (diurnal) variation was not identified in the serum t4 concentrations of the cats (p greater than 0.01). the lowest mean serum t4 concentration (9.1 micrograms/dl) was measured at 3 pm and was 14.2% l ...	1988	3343177
characterization of the thyroid hormone transport system of isolated hepatocytes.	transport of 3,5-[3'-125i]triiodo-l-thyronine ([125i]t3) was studied in isolated rat liver hepatocytes. t3 transport was temperature-sensitive, the initial velocity of uptake, at low substrate concentration, was 60 times higher at 25 degrees c than at 0 degrees c. the activation energy of cellular uptake (26 kcal/mol) was different from that of binding to cytosolic proteins (6 kcal/mol), indicating that the latter was not the rate-limiting step. uptake obeyed simple michaelis-menten kinetics, wi ...	1988	3343226
retrospective study of prognostic importance of dna flow cytometry of urinary bladder carcinoma.	cellular dna content was determined by flow cytometry on routinely processed paraffin sections of 61 primary and untreated transitional cell carcinomas of the urinary bladder, and correlated with tumour grade and stage and clinical follow up. all 16 (25%) grade 1 carcinomas were diploid and all 11 (20%) grade 3 tumours were aneuploid. the 34 (55%) grade 2 carcinomas comprised 13 (40%) diploid and 21 (60%) aneuploid cases. among the 37 superficial carcinomas (stage ta and t1), 25 (65%) were diplo ...	1988	3343376
fifty cases of human immunodeficiency virus (hiv) infection: immunoultrastructural study of circulating lymphocytes.	the peripheral lymphocytes of 50 cases of human immunodeficiency virus (hiv) infection (13 of acquired immune deficiency syndrome (aids), 17 of aids related complex (arc), and 20 healthy carriers) were studied immunoultrastructurally. the prevalence of "tubuloreticular structures" and "tubular confronting cisternae" increased with the progression of the disease. numerous tubular confronting cisternae were noted in patients presenting with a high serum acid labile alpha-interferon values. the pat ...	1988	3343380
independent effects of liver disease and chronic alcoholism on thyroid function and size: the possibility of a toxic effect of alcohol on the thyroid gland.	in an autopsy study we found thyroid volume significantly decreased in alcoholics with liver cirrhosis as compared to matched controls: 15 ml (range, 7 to 37 ml) v 25 ml (range, 13 to 90 ml) (p less than .01). at the same time the amount of fibrosis of the thyroid glands was higher in the alcoholics as compared to the matched controls: 20% (range, 6% to 40%) v 12% (range, 6% to 23%) (p less than .01). in order to evaluate the relative importance of alcohol consumption and liver disease on thyroi ...	1988	3343931
anal margin carcinoma: 21 cases treated at the institut curie by exclusive conservative radiotherapy.	between 1962 and 1980, 21 patients with anal margin carcinoma were treated with exclusive radiotherapy. they were divided into 4 t1, 7 t2, 8 t3 and 2 t4 cases; only 3 cases presented with an n1 lymph node involvement (1 t2 and 2 t3). the tumor dose was 65 gy in 22 fractions and 55 days; the inguinal dose was 50 gy in 15 sessions and 50 days for prophylactic irradiation performed in 9/18 n0 patients, and 65 gy with a limited 15 gy boost for the 3 n1 cases. the results are: for t1, 4 patients aliv ...	1988	3344351
the relation of thyroid indices in the critically ill patient to prognosis and nutritional factors.	thyroid indices and nutritional assessment values were measured in 73 critically ill euthyroid patients within 48 hours of admission to the medical or surgical intensive care unit. significantly increased rates of mortality were observed among patients with decreased t3 or t4 levels or elevated t3ur or rt3 values. alterations in thyroid function tests were associated with changes in certain putative nutritional indices, including serum albumin and transferrin concentrations, triceps skin fold an ...	1988	3344451
agenesis of the corpus callosum, combined immunodeficiency, bilateral cataract, and hypopigmentation in two brothers.	we describe 2 brothers with a malformation syndrome consisting of agenesis of the corpus callosum, cutaneous hypopigmentation, bilateral cataract, cleft lip and palate, and combined immunodeficiency. the clinical history of both patients was characterized by severe psychomotor retardation, seizures, recurrent severe respiratory infections, and chronic mucocutaneous candidiasis. the children died of bronchopneumonia at age 2 and 3 years, respectively. immunological investigations showed, in one s ...	1988	3344762
the temperature of bupivacaine 0.5% affects the sensory level of spinal anesthesia.	three milliliters of plain bupivacaine 0.5% was injected intrathecally in two groups of 20 patients. group 1 received a solution that had been equilibrated to 37 degrees c, group 2 received a solution that had been equilibrated to 4 degrees c. patients were kept sitting for 3 minutes after injection. all observations were observer-blind. the differences between segmental levels of sensory loss between groups 1 and 2 (t4 and t9, respectively) and of temperature loss (t3 and t8, respectively) 10 a ...	1988	3344977
improvement of diaphragmatic function by a thoracic extradural block after upper abdominal surgery.	the effects on diaphragmatic function of a thoracic epidural block were assessed in 13 patients after upper abdominal surgery (uas). lung volumes and tidal changes in chest wall circumferences and gastric (delta pgas) and esophageal (delta pes) pressures were measured pre- and postoperatively. volume displacement of the abdomen divided by tidal volume (delta vab/vt) and delta pgas/delta pes were taken as indices of the diaphragmatic contribution to tidal breathing. these respiratory variables we ...	1988	3344992
development of young rats and rabbits exposed to a strong electric field.	body growth and circulating levels of hormones were assessed in young rats and rabbits exposed to a 50-hz electric field of 50 kv/m. eight-week-old male rats were exposed 8 h/day for 4 weeks and rabbits were exposed 16 h/day from the last 2 weeks of gestation to 6 weeks after birth. the body and the organ growth of exposed rats were not statistically different from those of sham-exposed controls. no important differences from controls were observed in plasma levels of corticosterone, tsh, acth, ...	1988	3345217
transfer of thyroxine from the mother to the rat fetus near term: effects on brain 3,5,3'-triiodothyronine deficiency.	it has recently been shown that thyroid hormones are transferred from the mother to the developing rat embryo early in gestation, before the onset of fetal thyroid function. we have now studied whether there is transfer of t4 from the mother to the fetus late in gestation when the fetal thyroid is impaired. normal and thyroidectomized females were mated, given a goitrogen [methimazole (mmi)], starting before the onset of fetal thyroid function and until term, alone or together with a constant in ...	1988	3345725
euthyroid hyperthyroxinemia due to a generalized 5'-deiodinase defect.	we studied an 11-yr-old girl with asymptomatic hyperthyroxinemia, who remained euthyroid and healthy for 5 yr of follow-up. besides having elevated serum t4 concentrations, her serum free t4 concentrations were consistently elevated, as measured by three different methods, including equilibrium dialysis and ultrafiltration. serum total and free t3 concentrations were in the low normal range, and serum 3,5-diiodothyronine (3,5-t2) levels were low, suggesting reduced 5'-deiodination of both t4 and ...	1988	3346351
sex hormone-binding globulin in the diagnosis of peripheral tissue resistance to thyroid hormone: the value of changes after short term triiodothyronine administration.	thyroid hormone is one of several factors that modulate the level of sex hormone-binding globulin (shbg) in serum. shbg levels are usually elevated in thyrotoxicosis and have been reported to be normal in a few patients with generalized resistance to thyroid hormone (grth). this study was designed to determine whether basal serum shbg levels or the shbg response to short term t3 administration could be used as an index of thyroid hormone action and thus serve as a test for the evaluation of pati ...	1988	3346353
human chorionic gonadotropin (hcg) and thyroid function in early human pregnancy: circadian variation and evidence for intrinsic thyrotropic activity of hcg.	to explore the diurnal variation and the relationship between serum hcg levels and thyroid function during pregnancy, 26 women with an uncomplicated early pregnancy were studied before and after interruption of pregnancy. the high serum hcg levels in early pregnancy were accompanied by an increase in serum thyroid hormone and a decrease in serum tsh levels. nevertheless, serum tsh exhibited diurnal variation similar to that in nonpregnant women. the nocturnal surge of tsh exceeded the daytime na ...	1988	3346361
the serum concentrations of t3, t4 and tsh in evaluating replacement therapy in primary hypothyroidism.		1988	3347327
developmental changes in rat thyroid responsiveness to thyrotropin administered by the subcutaneous and peroral route.	it has been demonstrated that orally administered thyrotropin (bovine, btsh) evokes an increase in circulating t4 and t3 levels in 15-day-old suckling rat pups, but not in weaned animals. because the feedback mechanisms of the hypothalamo-pituitary-thyroid axis change dramatically during the neonatal period, we chose to examine the efficacy of exogenous btsh in eliciting a thyrostimulatory response via the subcutaneous (sc) or peroral (po) route in rat pups at 5, 8, 12, and 15 days postpartum. s ...	1988	3347611
[role of magnetic resonance in the staging and post-chemotherapeutic follow-up of malignant maxillo-facial tumors].	ten patients with squamous cell carcinomas located in the maxillo-facial region were studied. in all cases the staging by computed tomography (ct) and magnetic resonance (mri) revealed metastatic lesions in stage t3-t4. all patients underwent polichemotherapy before surgery. mri proved to be more accurate in the staging of tumors in 3 cases only, while in 7 cases both techniques showed the same diagnostic accuracy. in the follow-up, mri allowed the evaluation of the effect of chemotherapy on the ...	1988	3347789
ontogeny of thyroid hormone effect on tissue 5'-monodeiodinase activity in fetal sheep.	most of the thyroxine (t4) in fetal mammals is deiodinated to the inactive metabolite, reverse triiodothyronine (rt3), via an iodothyronine 5-monodeiodinase in fetal tissues. maturation of the tissue 5'-monodeiodinase (mdi) enzymes required for conversion of t4 to active triiodothyronine (t3) in the rat, an altricial species, occurs in the postnatal period. to characterize fetal maturation of the enzymes for active t3 production in a precocial species, 5'-mdi activities were measured in liver, k ...	1988	3348392
acute hypothyroidism in a severely ill surgical patient.	a case of acute postoperative hypothyroidism in a 62-year old woman is presented. one month before emergency admission because of a perforated gastric ulcer the patient had normal thyroid function, despite removal of a thyroid adenoma 20 years earlier. following surgery the patient developed circulatory instability, renal insufficiency, hypothermia and immeasurable concentrations of thyroxine (t4) and triiodothyronine (t3). treatment with triiodothyronine was commenced but the patient died follo ...	1988	3349557
dna amplification in vitro using t4 dna polymerase.	we have evaluated in vitro dna amplification by polymerase chain reaction using either t4 dna polymerase or klenow fragment of escherichia coli dna polymerase i. both polymerases under optimal salt conditions permit efficient amplification of exon 3 of the hypoxanthine guanine phosphoribosyltransferase (hprt) gene from human genomic dna and from plasmid containing the hprt cdna. dna sequences amplified from human genomic dna, using two 20-nucleotide primers flanking the ends of the exon, showed ...	1988	3349906
deoxycytidylate hydroxymethylase gene of bacteriophage t4. nucleotide sequence determination and over-expression of the gene.	we describe two approaches to cloning and over-expressing gene 42 of bacteriophage t4, which encodes the early enzyme deoxycytidylate hydroxymethylase. in bochum a library of sonicated fragments of wild-type phage dna cloned into m13mp18 was screened with clones known to contain parts of gene 42. two overlapping fragments, each of which contained one end of the gene, were cleaved at a hincii site and joined, to give a fragment containing the entire gene. in corvallis a 1.8-kb fragment of cytosin ...	1988	3350013
effects of propylthiouracil (ptu) on the immunological status of the chicken.	effects of propylthiouracil (ptu) using different treatment schedules on immune functions in young male chicken were studied. antibody productions to sheep red blood cells (srbc) and brucella abortus (ba) were found to rise in the chicks treated with 0.1% and 1% ptu. furthermore, the chicks treated with 1% ptu exhibited a significantly greater reaction to phytohemagglutinin (pha) in skin test using the wing web than the control did. treatment of 5% ptu, however, inhibited the antibody production ...	1988	3350187
hyperfractionation for head and neck cancer.	between march 1978 and april 1984, 144 patients with 148 moderately advanced to advanced primary squamous cell carcinomas of the head and neck received treatment with curative intent with twice-a-day irradiation (120 cgy/fraction, 4-6 hour interfraction interval). eighty-eight percent of the patients had ajcc stage iii-iv cancers. one hundred and thirty-two patients received irradiation alone to the primary site with or without radical neck dissection, with surgery reserved for salvage. the tota ...	1988	3350719
hereditary and environmental influences on the variation of thyroid hormones in normal male twins.	heritability of the variation of the plasma total and unbound t4 (free t4), t3, t4-binding globulin (tbg), and tsh concentrations was investigated in 15 monozygotic and 15 dizygotic male twin pairs. the variability in plasma of total (58%) and free t4 (72%) concentrations was significantly less (p less than 0.01) in the twin pairs than in unrelated men. half of the variability of t3 (p less than 0.05), tbg (p less than 0.05), and tsh (p less than 0.05) was affected by influences shared by the tw ...	1988	3350909
effect of mouth rinsing with two polyvinylpyrrolidone-iodine mixtures on iodine absorption and thyroid function.	a prospective study was conducted to investigate the effect of long term therapy with two iodine-containing mouth rinses on thyroid function. two groups of subjects were treated daily for 6 months with either a 5% polyvinylpyrrolidone (pvpi)-1.5% h2o2 mixture (perimed) or a 5% pvpi-water mixture. thyroid function studies, serum iodine concentrations, and urinary iodine excretion were measured before treatment, at 6-week intervals during the 6-month treatment period, and 3 weeks after the last tr ...	1988	3350910
combined radiotherapy and chemotherapy in stage t3 and t4 nasopharyngeal carcinoma in children.	twelve children younger than 16 years affected by undifferentiated nasopharyngeal carcinoma (npc) with advanced primary tumor (t3, t4) were treated with chemotherapy consisting of adriamycin (adm [doxorubicin; adria laboratories, columbus, oh]), vincristine (vcr), and cyclophosphamide (cyc), and radiotherapy. preradiation chemotherapy produced partial responses in eight of ten evaluable patients. eleven of 12 patients achieved a complete response following radiotherapy. the actuarial 3-year cont ...	1988	3351559
concomitant therapy with infusion of cisplatin and 5-fluorouracil plus radiation in head and neck cancer.	we have combined cisplatin, 5-fluorouracil infusion, and radiation in an every-other-week schedule in a phase i-ii study of 44 patients with head and neck cancer to assess toxicity and response. ten patients were treated palliatively with 2 to 6 cycles of therapy, and 34 were treated curatively with a planned 7 cycles. of 34 patients treated curatively, all were initially controlled. three died during treatment (1 myocardial infarction, 1 bowel perforation, and 1 renal failure after amino-glycos ...	1988	3352779
new strategies for avoiding total laryngectomy in patients with head and neck cancer.	cisplatin-based chemotherapy has yielded high response rates in patients with head and neck cancer but has failed to improve the survival rates in randomized studies. thus, its place in the management of head and neck cancer remains unclear. we have been investigating whether the combination of chemotherapy and radical irradiation as an alternative to morbid surgery in selected patients might yield an improved quality of life without compromising survival. between january 1983 and december 1985, ...	1988	3352784
thyroxine inner ring monodeiodinating activity in fetal tissues of the rat.	we studied thyroxine (t4) inner ring monodeiodinating activity (5-ma) in various tissues of fetal, maternal, and adult male rats. tissue homogenates were incubated with 0.26 microm t4 in 0.1 m phosphate buffer (ph 7.4) containing 10 mm edta and 400 mm dithiothreitol (final volume 0.7 ml) for 10 min at 37 degrees c; the 3,3',5'-triiodothyronine (rt3) generated was measured by radioimmunoassay of ethanol extracts of incubation mixture and the result was corrected for rt3 degradation during incubat ...	1988	3353163
bladder cancer prognostic indices: analysis of 286 patients treated with radical radiation therapy.	from 1974 through 1982, 286 patients with histologically proven bladder cancer (tis 8, t1 62, t2 109, t3 86, t4 21 nxmo) were treated with external irradiation after transurethral resection. irradiation was given with 260 degrees arc technique photon beam from a cobalt unit; the tumor doses ranged from 52.5 to 65 gy in 21-50 days, with a tdf between 75 and 110. the analysis was conducted with the log rank test on the cumulative percent survival (cps) at 5 years. the study pointed out the importa ...	1988	3354067
vip and its homologues increase vascular conductance in certain endocrine and exocrine glands.	the effects of vasoactive intestinal peptide (vip) and related structural homologues on tissue vascular conductances were investigated in anesthetized male rats. vip, peptide histidine isoleucine (phi), secretin, growth hormone-releasing factor (ghrf), gastric inhibitory peptide (gip), or saline was infused intravenously over 4 min. tissue blood flows were measured during this time by use of 141ce-labeled microspheres. regional blood flows were normalized for any change in mean arterial blood pr ...	1988	3354661
spinal anesthesia and lumbar lordosis.	hyperbaric bupivacaine 0.5% (3.0 ml) was injected intrathecally in two groups of 20 patients. both groups of patients lay in the lateral position with their hips flexed at 90 degrees. in group f, the hip flexion was maintained for 5 minutes after turning supine. in group s, the hips were straightened before the patients were turned to the supine position. the technique of hip flexion to reduce the lumbar lordosis did not significantly limit the height of anesthetic blockade. the distribution of ...	1988	3354867
early cancer of the gastric remnant.	early carcinoma of the gastric remnant was diagnosed in 19 patients between january 1976 and january 1986. in all patients early cancer was suspected at endoscopy and confirmed by biopsy and histology. the main reason for endoscopic examination was diffuse epigastric pain suggestive of stump gastritis. the surgical procedure was stump gastrectomy. two of the 19 patients were not operated on because of advanced age. in contrast with the poor prognosis of patients with cancers of the gastric remna ...	1988	3356360
a study of pituitary thyrotropin, its subunits, and messenger ribonucleic acids in nonthyroidal illness.	we studied serum tsh, pituitary tsh and alpha and beta-subunits of tsh and their messenger ribonucleic acids (mrnas) in three models of nonthyroidal illness (nti) in the rat, ie diabetes mellitus (1 wk after 65 micrograms streptozotocin/g bw ip), turpentine oil-injection (8 to 48 hours after after a dose of 5 microliter/g bw sc), and complete fasting for 72 hours. euthyroid, hypothyroid (two months after thyroidectomy) and hyperthyroid rats (30 micrograms t4/d x 7, sc) were also studied for comp ...	1988	3357421
nucleotide and deduced amino acid sequence of bacteriophage t4 gene 12.		1988	3357780