Publications

spontaneous mammary tumors in mongrel dogs. a relevant model to demonstrate tumor therapeutical success by application of neuraminidase-treated tumor cells.		1977	608530
toxicological studies with therapeutically-applicable vibrio cholerae neuraminidase.	neuraminidase was submitted to a preclinical toxicological investigation in short--and long--term experiments. the acute toxicity in mice, rats, and guinea pigs is low (ld 50 i.v.: mouse 30,000 units/kg, rat 44,000 units/kg, guinea pig 7,700 units/kg; s.c.: mouse, rat, guinea pig more than 30,000 u/kg). after i.v. injection into dogs, respiration is not at all and blood pressure only slightly altered. neuraminidase is pyrogenfree in the rabbit test. assays of antigenicity in guinea pigs and test ...	1977	608531
an investigation of cholora outbreak in raipur district.		1977	608722
carrier state in cholera.		1977	608772
an epidemiological approach to the study of the role of carriers and natural water sources in the maintenance of cholera infection in an endemic area in calcutta.		1977	608773
[non agglutinable cholera vibrios (nag) in sewage, riverwater, and seawater (author's transl)].		1977	610254
studies of the polysaccharide fraction from the cell wall lipopolysaccharides (o-antigens) of vibrio cholerae.		1977	612536
"vibrio cholerae" nag strains typable with cholera phages.		1977	616256
bacteriology and laboratory diagnosis of cholera.		1977	617670
[nonspecific infection in patients with tuberculosis of the urinary tract during antibiotic therapy].		1977	415177
[serologic investigations in a village of haute volta at the end of a cholera epidemic].		1977	579326
ecological study of vibrios in tiber, aniene and small rivers in the rome outskirts.		1977	579327
[ergonomy and nursing care: how to chose adequate equipment].		1977	242442
effectiveness of neuraminidase in experimental immunotherapy of two murine pulmonary carcinomas.	the effects of direct intratumoral inoculation with vibrio cholerae neuraminidase and inoculation of tumor-bearing mice with tumor cells incubated with neuraminidase in vitro were studied in c57bl/6 x dba/2 f1 mice bearing s.c.-transplanted, methylcholanthrene-induced pulmonary squamous cell or lewis lung carcinomas. the growth of the squamous cell tumor was more greatly inhibited by both treatments than was the lewis lung tumor. in the squamous cell tumor-bearing mice, both modes of neuraminida ...	1977	830425
activation of the nigrostriatal dopaminergic pathway by injection of cholera enterotoxin into the substantia nigra.	twenty-four hours after unilateral injection of cholera enterotoxin into the rat substantia nigra there is an increase, in the striatum on the injected side, of basal adenylate cyclase activity, 3,4-dihydroxyphenylacetic acid, and 3-methoxy-4-hydroxyphenylacetic acid. moreover, there is an increase of motor activity, and rats tend to circle contralateral to the side of the injection. injection of cholera enterotoxin into brain nuclei may be a useful procedure for pharmacologically activating sel ...	1977	831258
primary structure of cholera toxin beta-chain: a glycoprotein hormone analog?	the completed sequence of the beta-chain of cholera toxin (103 amino acid residues) was compared to the beta-chains of chorionic gonadotropin, thyrotropin, luteinizing, and follicle stimulating hormones. the overall chemical similarity of the toxin beta-chain to the hormones was not statistically different from random; however, a comparison of the first 40 residues of the toxin beta-chain to the glycoprotein hormones revealed a segment of the hormones which was significantly chemically similar. ...	1977	831277
trimethoprim-sulphamethoxazole in the treatment of cholera. comparison with tetracycline and chloramphenicol.	67 of the bacteriologically proved adult acute cholera patients have been examined in order to evaluate the efficacy of tm-smx in comparison with tetracycline and chloramphenicol in the eradication of vibrio cholerae from stools. our results demonstrated that all three drugs sterilized the stools of all patients within 3 days with the exception of one case of tm-smx's group, which had negative culture stools after 4 days. on the basis of our experience it can be emphasized that tm-smx can suppor ...	1977	832549
effect of the a and b protomers of choleragen on release of trapped glucose from liposomes containing or lacking ganglioside gm1.	liposomes containing trapped glucose were used to examine the interaction of the a and b protomers of choleragen with ganglioside gm1 and lipid model membranes. the b protomer (choleragenoid) was as effective as choleragen in causing release of trapped glucose from liposomes containing gm1; the a protomer did not release glucose from gm1 liposomes. neither choleragen nor the a or b protomers caused release of trapped glucose from glycolipid-free liposomes. anti-choleragen and complement, however ...	1977	833153
functional and metabolic properties of human asialofibrinogen.	the role of sialic acid in the functional and metabolic properties of purified human fibrinogen was investigated. fibrinogen treated with vibrio cholerae neuraminidase released 90 percent of its sialic acid without evidence of proteolysis, as indicated by the presence of intace a alpha, b beta, and gamma chains on sodium dodecylsulfate (sds)-polyacrylamide gels of the reduced asialoprotein. the thrombin and reptilase clotting times of human asialofibrinogen were shortened compared to those of no ...	1977	833473
the effect of anaerobiosis and bile salts on the growth and toxin production by vibrio cholerae.	environemntal conditions which might be present in the human intestinal lumen, such as anaerobiosis, a temperature of 37 degrees c and the presence of bile salts, were examined for their effects on the growth and toxin production by vibrio cholerae strains 569b and b1307 in syncase and in peptone water media. using aerobic conditions at 30 degrees c, which are commonly used for enterotoxin production, toxin (5 mug ml(-1)) and pleomorphic cells were detected during the exponential phase of the gr ...	1977	833574
interactions of intestinal lymph flow and secretion.	the relationship between lymph flow and intestinal secretion was studied in an isolated, vascularly perfused cat ileal preparation in which secretion was caused by three different means: 1) plasma dilution from a constant infusion of tyrode solution (2.5 ml/min per kg), 2) elevation of intestinal venous pressure to 30 mmhg, and 3) exposure to cholera toxin. in the plasma-dilution group, lymph flow attained a peak value of 38 times control at 60-90 min following the onset of the infusion, after w ...	1977	835698
primary structure of cholera toxin b-subunit.		1977	836280
effect of protonated 2,4,6-triaminopyrimidine, a tight junction blocker, on intestinal transport in dog ileum in vivo.	previous in vitro experiments suggest that protonated 2,4,6-triaminopyrimidine (tap+) inhibits passive na+ movement across tight junctions of various epithelial tissues. so far no evidence has been found that tap+ interferes with other mucosal transport processes. because blockage of the tight junctions would be a promising tool in studying intestinal transport physiology, the effect of tap+ was investigated in the dog ileum in vivo. when tap+ was added to a sodium-free mannitol solution, the tr ...	1977	838223
purification of flagellar cores of vibrio cholerae.	a procedure is described for the purification of the cores of flagella sheared from vibrio cholerae. v. cholerae is a monotrichous organism whose flagellar core (fc) is enclosed within a sheath. the purification procedure consists of several cycles of differential centrifugation and cesium chloride density-gradient ultracentrifugation in the presence of a neutral detergent, triton x-100. purity of the fc preparations is assessed by electron microscopy, polyacrylamide gel electrophoresis, and che ...	1977	838680
surface molecules of hematopoietic stem cells: requirement for sialic acid in spleen colony formation.	enzymatic treatment was used to test the function of some surface peptides and carbohydrates in hematopoietic spleen colony formation. proteases and most glycosidases had no effect on spleen colony formation, whereas treatment with vibrio cholerae neuraminidase reduced colonies by one-half. intact sialic acid (residues appear to play an important role in colony formation.	1977	841316
experimental toxigenicity of nag vibrios.	some strains of nag vibrios isolated from the stool of patients with diarrhoeal disease as well as from surface water caused an accumulation of fluid in the ligated rabbit gut loop. 5-fold concentrated sterile culture filtrates of some strains were found positive in this test as well. the volume of the accumulated fluid in gut loops injected with live cultures as well as with concentrated culture filtrates was apparently smaller than the volume accumulated after injection of non-concentrated v.c ...	1977	842178
studies of the ligand binding to cholera toxin, ii. the hydrophilic moiety of sialoglycolipids.	the binding between cholera toxin or its b-protein subunit and various ganglioside-related oligosaccharides was studied by equilibrium displacement dialysis. at low concentrations of ligand, the binding of monosialo-gangliotetraitol exceeded that of the parent ganglioside ii3neuacggose4-cer, the possible cell surface receptor for the toxin. the terminal galactose residue and an intact carboxyl group of the sialic acid moiety of monosialo-gangliotetraose were found to be necessary for strong bin ...	1977	844799
immunoglobulin and specific-antibody synthesis in vitro by enteral and nonenteral lymphoid tissues after subcutaneous cholera immunization.	an in vitro culture technique with synthesis of 14c-labeled protein has been used to study immunoglobulin and specific-antibody formation by spleen, mesenteric lymph nodes, peyer's patches, and small intestine of rabbits, which were immunized twice subcutaneously with vibrio cholerae lipopolysaccharide (lps) and enterotoxin; saline-injected rabbits served as controls. newly synthesized immunoglobulin g (igg), iga, and igm were quantitated by liquid scintillation after their isolation by means of ...	1977	844901
evaluation of surface components of vibrio cholerae as protective immunogens.	surface components of a motile inaba strain (ca401) were removed from washed cells by low-speed shearing. flagella contaminated with a vesicular material (designated as crude flagella [cfa1) were obtained by differential centrifugation of the shear fluid. vesicles were obtained from a nonflagellated mutant by the same procedure. homogeneous small vesicles were obtained in diminished yield from cscl gradients of cf preparations. treatment of cf with sodium deoxycholate removed the vesicular mater ...	1977	844905
neuraminidase and tumor immunotherapy.	preliminary results of first clinical studies with the enzyme neuraminidase call attention to a new kind of cancer treatment. this promising approach to tumor immunotherapy was entered into the clinical phase as a consequence of successful experimental studies in tumor-bearing mice, rats and dogs. in this review, the presently known and essential results of experimental and clinical studies on tumor immunotherapy by means of neuraminidase are presented as well as some necessary and critical cons ...	1977	846180
cholera in portugal, 1974.i. modes of transmission.	in april-november 1974, portugal had a cholera epidemic caused by vibrio cholerae el tor inaba with 2467 bacteriologically confirmed hospitalized cases and 48 deaths. most of the country was affected, with 17 of the 18 districts reporting cases. v. cholerae was isolated from 42 per cent of shellfish tested during the epidemic, and an epidemiologic study found that a history of consumption of raw or poorly cooked cockles was significantly more common among cholera patients than among paired contr ...	1977	848483
cholera in portugal, 1974. ii. transmission by bottled mineral water.	during a cholera epidemic, vibrio cholerae was isolated from two springs which supplied mineral water to a spa and to a commercial water bottling plant. epidemiologic investigation found that cholera attack rates were 10-fold greater among visitors to the spa than among non-visitors. a subsequent matched-pair case-control study which excluded persons who had visted the spa showed that a history of consumption of the bottled non-carbonated water was significantly more common among bacteriological ...	1977	848484
cholera on guam, 1974: epidemiologic findings and isolation of non-toxinogenic strains.	in august 1974, six cases of cholera occurred on guam. the index case had severe diarrhea and metabolic acidosis and died from pneumonia on the ninth day of illness; the other five cases had only mild to moderate diarrhea. fish caught in agana bay and home-preserved was found to be the vehicle most likely responsible for the cases. vibrio cholerae, el tor ogawa, was isolated from two patients, the guam sewerage system, and a river emptying into agana bay. v. cholerae, el tor inaba, was isolated ...	1977	848485
[effect of a polysaccharide-contaning preparation from el tor vibrions on the phagocytic activity of leukocytes and on the lysozyme titer].	the dynamics of changes in some indices of the natural immunity of mice treated with el-tor vibrio preparations in doses of 50 and 500 gemma/mouse was studied. the blood for analysis was taken from the mice 24 hours, 3, 7 and 10 days after the preparation administration. the results of the study showed that administration of the polysaccharide-containing preparations from el-tor vibrios in doses of 50 and 500 gemma/mouse was accompanied by some shifts in the regulatory mechanisms: suppression of ...	1977	848918
morphology of vibrio cholerae during enterotoxin production under anaerobic conditions.		1977	850292
epithelial cell extrusion during fluid transport in canine small intestine.	epithelial cell extrusion during fluid transport was studied under both in vitro and in vivo conditions. the rate of cell extrusion from the villus tips in vitro increased by about onefold in the villi with obstruction of lymph flow associated with the increase of lymph and tissue fluid pressure. when lymph pressure in the jejunal and ileal villi was increased to 6.4 +/- .2 and 12.3 +/- .5 mmhg, respectively, by injection of ringer solution into the central lacteals, fluid leaked out of the vill ...	1977	851184
preparation and characterization of armadillo submandibular glycoproteins.	the nine-banded armadillo (dasypus novemcinctus mexicanus peters) was chosen for this study so that a comparison could be made of the salivary mucus glycoproteins of an ancient mammalian species with those derived from previously studied, more highly evolved species. two mucus glycoproteins, armadillo submandibular glycoprotein a and armadillo submandibular glycoprotein b, were prepared from the armadillo submandibular gland by a modification of the method of tettamanti & pigman (1968) (arch. bi ...	1977	851423
selective isolation of vibrio cholerae neuraminidase using an immobilized 4-(nitrophenyl)oxamic acid.	n-(4-nitrophenyl)oxamic acid[1] (1) was coupled with sepharose 4b containing 1,6-diaminohexane as spacer group. this material was used as a specific adsorbent in the purification of vibrio cholerae neuraminidase. the enzyme was completely retarded and separated from the bulk of the protein when washed with 50mm sodium acetate buffer, ph 5.0. a stepwise increase of sodium chloride concentration from 1.0 to 2.0m was found to be necessary for a sharp elution of neuraminidase activity. the purificat ...	1977	852823
[comparative study of the physico-chemical properties of peptones used to the production of dry alkaline agar for cholera diagnosis].	the authors present the results of chemical and physico-chemical study of dry peptones (made in the ussr) used in the production of dry alkaline nutrient agar for the diagnosis of cholera, in comparison with peptones used for other purposes. the batches and types of peptones studied differed by the fractional peptide composition and by the physico-chemical characteristics. positive results were obtained in the biological test in using peptones with marked hydrolysis with the amine nitrogen conte ...	1977	855517
r plasmids from asian strains of vibrio cholerae.	five r plasmids transferred from asian strains of vibrio cholerae all proved to be members of compatibility group c. a non-self-transmissible plasmid, stable in v. cholerae, was mobilized for transfer to escherichia coli k-12 and found to be unstably inherited in that host. plasmids of group c and p transferred to a wild v. cholerae strain were stably inherited.	1977	856010
inhibition studies on vibrio cholerae neuraminidase.	a series of viral neuraminidase inhibitors showing no structural analogy to neuraminic acids have been tested to find whether they are effective inhibitors of v. cholerae neuraminidase, too. here we report the results obtained with the n-phenyloxamic acid derivatives 2 to 6 (r-nh-co-coor'; r = -c6h5no2, -c6h5oh, -c6h5nh2; r' = -h, -c2h5; see table 1) and with simple aromatic compounds structurally related to r, i.e. 4-nitroaniline (7), n-acetyl-4-nitroaniline (8), 4-nitrophenol (9), 2,4-dinitrop ...	1977	856712
antigenicity of cholera toxoid in humans.	a cholera toxoid was produced by heating of a purified cholera toxin and treatment of the resulting toxoid procholeragenoid with a formaldehyde solution. when given subcutaneously and intramuscularly to volunteers in doses of 3-100 microng, the toxoid produced no noteworthy side effects, but the injections produced a dose-dependent rise in level of toxin-neutralizing antibodies in serum. administration of toxoid in combination with whole-cell vaccine induced antitoxin levels that seemed to be hi ...	1977	856918
sucrose teepol tellurite agar: a new selective indicator medium for isolation of vibrio species.	sucrose teepol tellurite (stt) agar is a more successful medium in positive-recognition palting procedures than thiosulfate-citrate-bile salts-sucrose (tcbs) agar because stt agar yields higher isolation of typical colonies of vibrio cholerae, which directly agglutinate in antiserum to v. cholerae, from patients and contacts infected with this organism. stt agar has a simple composition and, like tcbs agar, needs no sterilization. stt agar is highly selective for v. cholerae, nonagglutinating vi ...	1977	856920
[cholera vibrio fractions. i. isolation of alkaline extracts of cholera vibrios and a study of their biological properties].	the authors suggest a well-reproducible method of preparation of a biologically-active fraction of an alkaline extract of cholera vibrio from the strain 569b of inaba serological type consisting in a preliminary triple removal of endotoxin (o-antigen) of the causative agent from the microbial cell suspension with trichloracetic acid with the subsequent extraction of the microbial mass and neutralization of the extract obtained capable of effective detection of the state of specific sensitization ...	1977	857521
a peripheral high molecular weight glycoprotein located at the surface of human platelets.		1977	858358
diagnostic antigens and antisera in tablet form.	diagnostic antigen suspensions and antisera can be prepared in tablet form with no loss in potency.	1977	858785
preparation of fixed antigenic, non-oncogenic and protective neoplastic cells.	transplantable spontaneous mammary adenocarcinoma and sarcoma p 1798 cells were incubated with vibrio cholera neuraminidase (vcn), then fixed cells demonstrated ability to exclude trypan blue dye and to immunized animals and produce cytotoxic sera of high titers. the fixed neuramindase-treated tumor cells became non-oncogenic and protected the host against high doses of fresh untreated homologous tumors.	1977	862758
anti-inflammatory effect of berberine in rats injected locally with cholera toxin.		1977	863481
studies on streptomycin dependent mutants of vibrio cholerae.		1977	863486
cell-mediated immunity after oral immunization with ribonucleic acid-protein fractions of vibrio cholerae l-form lysates.	oral administration of a single dose of ribonucleic acid-protein fraction of lysates of vibrio cholerae subtype ogawa l-forms induced an increase in cell-mediated immunity in rabbits. this was demonstrated by an increase in leukocyte migration inhibition in peripheral blood leukocytes, in macrophages migration inhibition, and in microbicidal activity against listeria monocytogenes in peritoneal macrophages obtained from orally immunized rabbits. increased cell-mediated immunity was induced mainl ...	1977	863514
altered guanine nucleotide hydrolysis as basis for increased adenylate cyclase activity after cholera toxin treatment.		1977	863902
[cholerogenicity of l-forms of cholera vibrios].	dutta and habbu's method was applied to comparative study of cholerogenic properties of the l-strains of cholera vibrios and their initial bacterial variants. in difference from the initial strains, l-forms of cholera vibrios possessed no cholerogenic properties. a possibility of reversion of the stable l-form of cholera vibrios into bacterial form in vivo was revealed. this reversion was accompanied by the restoration of the cholerogenic properties and the change of the biotype of the cholera v ...	1977	868409
[reversion of l forms of non-agglutinating vibrios and biological characteristics of the revertant cultures].		1977	868410
amino sugar contents and phage inactivating properties of lipopolysaccharide from cholera and el tor vibrios.	cholera and el tor lipopolysaccharide (lps) were identical in respect of chemical composition except that the hexosamine content was significantly lower and galactosamine was absent in el tor lps. mukerjee's group iv cholera phage phi149 was inactivated by cholera lps but was resistant to el tor lps.	1977	869403
intestinal fluid accumulation induced by oral challenge with vibrio cholerae or cholera toxin in infant mice.	the diarrheal response of orally inoculated infant mice to viable vibrio cholerae and purified cholera toxin was quantitated by means of a fluid accumulation (fa) ratio. the fa ratio is defined as the gut weight/remaining body weight. fa ratios were determined in relation to time of exposure and dose. onset of fluid accumulation with viable cells of strains ca401 and 569b occurred 8 h postinoculation and reached a near maximum of 16 h. a dose of 4 x 10(6) colony-forming units of strain ca401 was ...	1977	870427
migrating action potential complex of cholera: a possible prostaglandin-induced response.	distal ileal loops of new zealand white rabbits exposed to cholera toxin demonstrated the presence of a highly organized myoelectric pattern defined as the migrating action potential complex (mapc). we investigated the mechanism by which cholera enterotoxin stimulates mapc activity. certain anti-inflammatory drugs have altered the secretory component of cholera diarrhea. we investigated effects of these anti-inflammatory drugs on the mapc. indomethacin, 5.0 mg/kg iv, abolished all mapc activity. ...	1977	871161
vcn-releasable sialic acid and gangliosides in human neuroblastomas.	the ganglioside pattern and vcn-releasable sialic acid residues of six human neuroblastomas were studied. there was no correlation between vcn-releasable sialic acid and prognosis. the ganglioside patterns were more complex in those cases with an expected good prognosis as reflected by the presence of trisialoganglioside. the complexity of the ganglioside pattern did not always correlate with the histologic grade of the tumor. these results suggest the ganglioside pattern may serve as a chemical ...	1977	874729
lipopolysaccharides of vibrio cholerae--chemical and immunological properties--.		1977	875239
action of some pancreatic enzymes on vibrio cholerae.	the action of pancreatic amylase, trypsin, lipase, and whole pancreatin was tested on five strains of bibrio cholerae. amylase did not act on any strain in concentrations to 10,000 iu/ml whereas trypsin increased vacuolization and lipase enhanced retraction of the protoplasm particularly in 2 of the 5 tested vibrio strains. pancreatin caused damage hoth of the cell wall and the cytoplasm. it is suggested that these enzymes may play a role in the defense of the body against cholera vibrios.	1977	875887
recent advances in etiology, pathophysiology, immunology of pediatric cholera in relation to treatment and prevention.		1977	876664
stimulation by immune complexes of mucus release from goblet cells of the rat small intestine.	immune complexes (bovine serum albumin with rat antibodies to bovine serum albumin) formed in twofold antibody excess were injected into the duodenum of normal rats. in comparison to controls injected with antigen only, there was a marked increase in the percentage of disrupted goblet cells (an index of mucus release) in segments from the intestine of rats exposed for 3 hours to immune complexes in vivo. similarly, there was a significant increase in 35s-labeled mucus recovered by filtration of ...	1977	877559
incidence and elimination of r plasmids in vibrio cholerae.	of 124 strains of vibrio cholerae, 32 were multiply resistant to antibiotics. this resistance appeared to be determined by r plasmids on the basis of their effective elimination by sodium dodecyl sulfate, acridine orange, ethidium bromide, and ultraviolet radiation.	1977	879755
heterogeneity of purified cholera toxin.	the heterogeneity of vibrio cholerae toxin, obtained from culture filtrates in homogeneous form by gel filtration and preparative disc gel electrophoresis has been studied. by means of disc electrophoresis on polyacrylamide gel cholera toxin was separated into three forms designated i (5%), ii (15%) and iii (80%). the toxic activity, amino acid content and molecular weight of the three forms were similar. the difference so far observed between the various electrophoretic fractions is a differenc ...	1977	884132
macrophage plasminogen activator: induction by concanavalin a and phorbol myristate acetate.	the synthesis and secretion of plasminogen activator by cultured macrophages can be induced and stimulated by concanavalin a and by phorbol myristate acetate, and inhibited by such agents as glucocorticoids, mitotic inhibitors and compounds affecting camp metabolism. by the manipulation of stimulatory and inhibitory influences, enzyme production can be modulated continuously over a 200 fold range. in the same way, the proportion of cells that secrete detectable levels of enzyme can be varied fro ...	1977	884740
enhanced non-electrolyte uptake in vitro by the dog jejunal mucosa in the cholera-toxin-induced secretory phase.	a characteristic secretory response in the dog jejunumis observed following three hours' perfusion with cholera toxin. when this mucosa in the secretory phase is examined in vitro, the steady-state accumulation of phenylalanine and beta-methyl-glucoside is considerably enhanced. since the initial rates of influx of these substrates are unchanged and the tissue ion contents are the same as normal, it is concluded that the increase in steady-state uptake may be attributed to a change in the rate o ...	1977	886482
study of intestinal immunity against v. cholerae: role of antibody to v. cholerae haemagglutinin in intestinal immunity.	cell-bound haemagglutinin as an adhesive factor of vibrio cholerae has been partially purified from e1 tor vibrios using 0.05 m cyclohexylaminopropane sulfonic acid buffer ph 9.5 and gel filtration column chromatography. rabbits were immunized with the precipitin complexes of the haemagglutinin and its antibody. the antiserum is tested for the protective ability against the oral challenge with vibrio cholerae strain of which the haemagglutinin has been prepared. the results indicate definite pro ...	1977	887992
simple method for purifying choleragenoid, the natural toxoid of vibrio cholerae.	choleragenoid, a nontoxic aggregate of the b subunit of cholera toxin, has been purified from concentrated culture filtrates in a single step by ion-exchange chromatography on phosphocellulose or other cation-exchange resins. this procedure is far simpler than others currently used to isolate choleragenoid and yields a preparation essentially free from nucleic acid, lipopolysaccharide, toxin, and other proteins present in the crude culture filtrates. the purified choleragenoid retained the speci ...	1977	892899
immune response of the intestinal mucosa to cholera toxoid.	the intestinal immune response to cholera toxoid was studied in dogs and rats. oral or intraperitoneal priming followed by duodenal boosting with toxoid reulted in antitoxin-containing plasma cells (acc) in jejunal lamina propria of rats. priming and boosting by the intraperitoneal route alone induced almost no jejunal response. lamina propria acc were derived largely from migrating immunoblasts, which appeared earlier among thoracic duct lymphocytes. protection of dogs after repeated subcutaneo ...	1977	894085
enterotoxicity of aeromonas hydrophila.	live cells and cell-free culture supernates of 50 strains of aeromonas hydrophila isolated from diarrhoeic and healthy human faeces, drinking water, sewage, the river ganges and faeces from domestic animals caused accumulation of fluid in ligated ileal loops of adult rabbits. the amount of fluid produced was comparable to that of a toxigenic strain of vibrio cholerae. three of the strains gave positive reactions only after two passages in ileal loops of rabbits. inocula of about 10(3) viable cel ...	1977	894697
interaction of vibrio cholerae el tor and gut mucosa in ligated rabbit ileal loop experiment.	light and electron microscope studies in ligated rabbit gut loops showed that cholera vibrios el tor attached themselves to the enterocytes and multiplied, most probably on the intestinal epithelium surface. but they did not penetrate deeper and were unable to cause destruction of the epithelium or marked inflammation. ultrastructural manifestations of both apocrine and merocrine secretion of the enterocytes were observed. in addition, the enterocytes showed signs of generally enhanced activity. ...	1977	895212
[interactions of the cholera vibrion and its toxins with the body on the ultrastructural level].		1977	895581
[classification of acute bacterial enteropathies].	the first part considers pathogenic microorganisms (vibrio cholerae and parahaemolytic vibrio, clostridium welchii, enteropathogenic e. coli, shigella, salmonella, other enterobacteria and pseudomonas. yersinia, simply enterotoxic staphylococcus and that producing acute enteritis) and the process of infection (formation of a surface link without endocellular penetration with elaboration of hexotoxins, formation of a surface link with subsequent intracellular penetration, submucosa penetration). ...	1977	896078
cholera toxin crystals suitable for x-ray diffraction.	large crystals of the cholera toxin were grown; their dimensions, symmetry (p21), order, and resistance to radiation make them ideally suited for a high-resolution x-ray structure determination. there is one molecule (approximately 84,000 daltons) per asymmetric unit, and therefore the lattice reveals no molecular symmetry. two distinct bioassays indicate that the protein from dissolved crystals retains full biological activity.	1977	897666
[electron microscopic study of the structure of cholera bacteriophage serotype ii].	the methods of electron microscopy and optic difraction were used to determine the main parameters of the structural organization of cholera bacteriophage of serotype ii. the virions of the phage under study were found to consist of a capsid of icosahedral shape and 77.4 x 66.1 nm in size, formed of 252 morphological subunits and having the cavity volume of 76.3 x 10(3) nm3. a process of 94.8 nm in length adheres to the capsid, with a shell capable of contractions, of 85.6 nm in length, 20.6 nm ...	1977	898900
[role of adrenal cortex hormones in the realization of the biological action of cholerogen].	experiments were conducted on albino mice, guinea pigs, and rabbits. the role played by glucocorticoids in the realization of the biological action of cholerogen--cholera exotoxin (edema of the limbs in mice and the skin test after craig in guinea pigs and rabbits) was studied. tissue sensitivity of these animals to the action of cholerogen diminished under the effect of stress factors (intramuscular injection of formalin, intraperitoneal injection of cholerogen, immobilization, hunger, laparato ...	1977	899424
[comparison of the dna polynucleotide sequence of cholera so-called non-agglutinating vibrios].		1977	899451
[restoration of the agglutinability of cholera vibrios in the bodies of guinea pigs].		1977	899452
cross linking of deoxyribonucleic acid in furazolidone treated vibrio cholerae cell.		1977	901562
[the 7th cholera pandemic].		1977	904730
vibrio cholerae, vibrio parahaemolyticus, and other vibrios: occurrence and distribution in chesapeake bay.	vibrio cholerae was isolated at several locations in chesapeake bay in fall 1976 and spring 1977. strains induced fluid accumulation in rabbit ileal loops and positive activity in y-1 adrenal cells. vibrio cholerae, vibrio parahaemolyticus, and related vibrios show a spatial and temporal distribution characteristic of vibrio species in an estuary. the vibrio cholerae strains isolated from chesapeake bay represent serotypes other than o-group i--that is, so-called nonagglutinable vibrios--and are ...	1977	910135
mitogenic action of neuraminidase.	a new effect of ncv on lymphocytes is demonstrated. this property is the capacity to act as a mitogen in and of itself. the possible mechanisms of this phenomenon are discussed.	1977	923734
transformation of vibrios with r factors from enterobacteria.		1977	924579
pathogenic mechanisms of a non-agglutinable vibrio cholerae strain: demonstration of invasive and enterotoxigenic properties.	a non-agglutinable vibrio cholerae strain isolated from the blood of a child with kwashiorkor and fever was shown to have the potential to invade as well as to produce a toxin resembling cholera toxin.	1977	924682
comparative antigenicity testing of cholera vaccines by application of hog gastric mucin and ferric ammonium citrate.		1977	925044
assay of cholera vaccine potency.		1977	925045
susceptibilities of bacteria against chemotherapeutics under different conditions.		1977	926525
age related differences in binding of concanavalin a to plasma membranes of isolated neurons.	neurons isolated from the lateral vestibular nucleus of young adult and senescent fischer-344 rats were incubated with fluorescamine-labelled concanavalin a (fl-con a) alone, or following incubation in trypsin or vibrio cholerae neuraminidase. they were then observed and photographed. microdensitometric analysis of fluorescence micrographs showed that senescent rat neurons were significantly more fluorescent than those from young adult rats. additionally, either patches or caps of fl-con a were ...	1977	930848
antigenicity of a spontaneous murine mammary adenocarcinoma during in vitro cultivation.		1977	23597
evaluation of the effectiveness of receptor destroying enzyme preparations.	a procedure has been developed for testing receptor destroying enzyme (rde) preparations used to remove nonspecific inhibitors before carrying out haemagglutination inhibition tests with influenza virus. four criteria should be taken into account: (1) titre of rde, usually used to indicate the activity of the preparation; (2) neuraminidase activity of the preparation, determined biochemically, which partially corresponds to the rde titre; (3) direct demonstration of the complete removal of nonsp ...	1977	23666
light-activated calcium release from sonicated bovine retinal rod outer segment disks.	calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. the amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insensitive to the internal trapped calcium concentration. the results are obtained using a flow system in which the disk membrane vesicles are adsorbed on glass particle supported by a ...	1977	14680
possible subdivisions among alpha-adrenoreceptors in various isolated tissues.	the ratio (expressed in log10 units) of the equieffective concentrations of (+)- and (-)-noradrenaline has been measured in a variety of isolated tissues in the presence of cocaine (1 x 10(-5) m), tropolone (3 x 10(-5) m) and (+/-)-propranolol (5 x 10(-7) to 5 x 10(-5) m). the values obtained fall into 3 distinct and statistically different groups. firstly, a high group comprising (mean +/- s.e.) mouse vasdeferens (2-78 +/- 0-04), rabbit duodenum (2-91 +/- 0-07) and ileum (2-86 +/- 0-05). second ...	1977	15074
ph measurements in the vascular wall of dogs with electrically induced thrombosis of the femoral artery.	electrically induced thrombosis is often used for pathophysiologic and therapeutic experiments. the ph value of the vascular wall was measured to approach the features of this thrombosis. after inducing thrombosis by direct current, ph value of the intima of femoral artery was found decreased. the anode was inserted in the lumen. as to the behavior of the ph value, electric thrombosis and clinical thrombosis are similar. electric thrombosis therefore seems to be valuable for comparing investigat ...	1977	15352
effect of gangliosides and substrate analogues on the hydrolysis of nicotinamide adenine dinucleotide by choleragen.	choleragen and its a protomer catalyzed the hydrolysis of nad to adp-ribose and nicotinamide. nadase activity was inhibited by gangliosides gm1 (galactosyl-n-acetylgalactosaminyl-n-acetylneuraminyl-galactosylglucosylceramide), gm2 (n-acetylgalactosaminyl-n-acetylneuraminyl-galactosylglucosylceramide), gm3 (n-acetylneuraminyl-galactosylglucosylceramide), and gd1a (n-acetylneuraminylgalactosyl-n-acetylgalactosaminyl-e1n-acetylneuraminyl-galactosylglucosylceramide). these gangliosides also increase ...	1977	13371
purification and properties of the methane mono-oxygenase enzyme system from methylosinus trichosporium ob3b.	1. a three-component enzyme system that catalyses the oxidation of methane to methanol has been highly purified from methylosinus trichosporium. 2. the components are (i) a soluble co-binding cytochrome c, (ii) a copper-containing protein and (iii) a small protein; the mol. wts. are 13 000, 47 000 and 9400 respectively. the cytochrome component cannot be replaced by similar cytochrome purified from pseudomonas extorquens or by horse heart cytochrome c. 3. the stoicheiometry suggests a mono-oxyge ...	1977	15544
hormonal control of zinc uptake and binding in the rat dorsolateral prostate.	the zinc uptake in the dorsolateral prostate of rats was studied after different hormonal manipulations. orchiectomy reduced the uptake of 65zn. administration of estradiol benzoate to orchiectomized rats doubled the 65zn uptake, a phenomenon which was not observed in orchiectomized-adrenalectomized rats. adrenalectomy in orchiectomized rats had no effect on the concentration of radioactivity beyond the castration-induced decrease. a prolactin release inhibitor, 6-methyl-8-erogelenylacetamide, r ...	1977	15955
alpha-isopropylmalate synthase from alcaligenes eutrophus h 16 i. purification and general properties.	alpha-isopropylmalate (ipm) synthase, the first enzyme in the biosynthesis of l-leucine, was purified to a specific activity of 12 micronmole/min x mg protein from the valine-isoleucine double auxotrophic mutant a-81 of the hydrogen bacterium alcaligenes eutrophus h16. the activity in crude extracts of derepressed cells was 0.106 micronmoles of isopropylmalate formed per min and per mg protein. gel electrophoresis and regel electrophoresis of the isolated main band resulted in several distinct b ...	1977	16576
purification and properties of a membrane-associated, folate-binding protein from lactobacillus casei.	a folate-binding protein has been solubilized from lactobacillus casei by treatment of membrane preparations with triton x-100 in the presence of 3hfolate. the protein-folate complex was purified 100-fold and recovered in a 22% yield by adsorption and elution from microgranular silica (quso g-32), followed by passage through sephadex g-150. when subjected to sodium dodecyl sulfate/polyacrylamide gel electrophoresis, the purified preparations showed only a single, protein-staining band whose mole ...	1977	16917
adenylate cyclase permanently uncoupled from hormone receptors in a novel variant of s49 mouse lymphoma cells.	a novel variant of the s49 mouse lymphoma has been selected from wild-type cells by growth in medium containing the beta-adrenergic agonist terbutaline and inhibitors of cyclic nucleotide phosphodiesterase. in contrast to the situation in the wild-type clone, synthesis of adenosine 3':5'-monophosphate (cyclic amp) is not stimulated by beta-adrenergic agonists or by prostaglandin e1 either in intact variant cells or in membrane preparations of such clones. however, basal and naf-stimulated activi ...	1977	17119
studies in acid-base balance. i. effect of alkali therapy in newborn dogs with mechanically fixed ventilation.	the effect of rapid or slow infusion of hypertonic sodium bicarbonate on acid-base balance and serum osmolality was studied in 36 acidotic newborn dogs. respiratory acidosis and hypoxia were produced by mechanically fixed hypoventilation. one group of animals breathed 100% o2 to prevent hypoxemia. rapid infusion of hco3- in acidotic and hypoxic animals resulted in only a transient (1 minute) and small (0.05 ph units) elevation of arterial ph followed by a continuous fall, resulting in a lower ph ...	1977	17663
cyclic guanosine-3',5'-monophosphate--mediator of the action of the hormaone erythropoietin.		1977	18327