Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| influence of tonicity and ph on lacrimation and ocular drug bioavailability. | 1978 | 29087 | |
| effects of psychotropic drugs on the spinal ventral root reflexes in cats. a comparison of the depressant actions in intact and spinal preparations (author's transl). | effects of the typical psychotropic drugs such as neuroleptics, tricyclic antidepressants and benzodiazepines on the monosynaptic reflex (msr) and polysynaptic reflex (psr) were investigated using intact and spinal cats. drugs used were chlorpromazine, levomepromazine, perphenazine, prochlorperazine, droperidol, haloperidol, amitriptyline, imipramine, diazepam and flurazepam. neuroleptics depressed the ventral root reflexes markedly to slightly in both preparations. the inhibitory effects of the ... | 1978 | 29826 |
| red cell aging. ii. anomalous electrophoretic properties of neuraminidase treated human erythrocytes. | desialylation of human red blood cells (rbc) by vibrio cholerae neuraminidase (vcn) was found to produce cells with electrophoretic properties which were inconsistent with the view of simple loss of n-acetylneuraminic acid (nana) as the sole effect of vcn treatment. modification of human rbc with 50--350 u vcn/10(10) rbc for one hour at 37 degrees c releases 90-100% of the nana and produces a progressive decrease towards zero in their electrophoretic mobilities when measured in 0.15 m nacl (ph 7 ... | 1978 | 29906 |
| the regulation of intracellular calcium by mitochondria. | 1978 | 30378 | |
| effect of endogenous and exogenous progesterone on human endometrial enzymes. | activities of 9 enzymes were determined biochemically in the endometrium. in trial i (five women) 25 mg progesterone were injected i.m. on day 9 of the cycle; and endometrial biopsy taken 24 hours later was compared with endometrium from day 10 and day 21, taken in two untreated cycles from the same volunteers. similarly, in trial ii (five women) 50 mg progesterone were injected on day 9, biopsy taken on day 11 and compared with days 11 and 21 from untreated cycles. the specific activites of lac ... | 1978 | 30706 |
| blockade by n-methylhydroxylamine of activation of guanylate cyclase and elevations of guanosine 3',5'-monophosphate levels in nervous tissues. | hydroxylamine and n-methylhydroxylamine prevented the activation of soluble guanylate cyclase by the endogenous activator as well as by nitroso compounds such as n-methyl-n'-nitro-n-nitrosoguanidine or nitroprusside, while other derivaties of hydroxylamine were ineffective. hydroxylamine and n-methylhydroxylamine did not alter the basal guanylate cyclase activity of purified enzyme preparations. kinetics analysis indicated that n-methylhydroxylamine competes with n-methyl-n'-nitro-n-nitrosoguani ... | 1978 | 31192 |
| (exo, exo)-2-aryltropane-3-carboxylic esters, hypoglycemic agents with accompanying analgesic activity. | (exo, exo)-2-aryltropane-3-carboxylic esters of types 6, 7, and 10 lower circulating blood glucose levels by 60--80%. this activity is accompanied by an analgesic activity roughly equal to that of codeine. both of these activities reside in the 1r enantiomer and extensive structure-activity studies failed to separate them. the specific opioid antagonist nalorphine blocks the analgesic activity but does not diminish the hypoglycemic action. conformational integrity afforded by the ethylene bridge ... | 1978 | 31482 |
| [the behaviour of some histochemical reactions in testicle tissue of a white rat as a result of kaptan action (author's transl)]. | 1978 | 94984 | |
| [cellular response in the mouse to two antigenic extracts from "vibrio cholerae" (author's transl)]. | at the cellular level the primary response in balb/c mice to an antigen prepared from a strain of vibrio cholerae ogawa was studied by a localized vibriolytic plaque procedures which permits the enumeration of immunocytes. the antibody response to a single injection with identical doses of antigen was comparatively studied by both intraperitoneal and subcutaneous immunization. the highest titre was noted two weeks after the intraperitoneal and three days after the subcutaneous injection. inhibit ... | 1978 | 568906 |
| isolation of a bacillus subtilis 168 derivative sensitive to defective bacteriophage pbsx. | a derivative of bacillus subtilis strain 168 sensitive to defective bacteriophages pbsx and pbsz has been isolated. this particular strain, rub824, carries the genetic information for defective bacteriophage pbsx. | 1978 | 415153 |
| effect of oral pancreatic enzymes on the course of cholera in protein-deficient vervet monkeys. | the effect of pancreatic extract on the course of cholera infection in protein-deficient vervet monkeys (cercopithecus aethiops) was studied. eleven animals were made protein deficient by diet. thier intestinal pancreatic enzyme concentration decreased as they became protein depleted. these animals were then challenged with vibrio cholerae. four control animals given a standard diet were similarly challenged. immediately after challenge, 6 of the protein-deficient monkeys were given a highly pur ... | 1978 | 415928 |
| brucellosis in elk i. serologic and bacteriologic survey in wyoming. | incidence of brucellosis in elk (cervus canadensis) on two winter feedgrounds in wyoming was examined over a 5-year period by testing serum samples using the standard plate agglutination (spt) buffered brucella antigen (bba), rivanol (riv) and complement fixation (cft) tests. thirty-one percent of 1,165 elk were positive by defined criteria. considering each test individually, only 29% (106) of 370 positive sera would have been classified as reactors by the spt, 83% (307) by the bba test and 86% ... | 1978 | 416232 |
| hypercomplexities in the visual cortex. | 1978 | 416357 | |
| isolation of nontoxinogenic mutants of vibrio cholerae in a colorimetric assay for cholera toxin using the s49 mouse lymphosarcoma cell line. | a mouse lymphosarcoma (s49) cell line that is growth-inhibited by agents that elevate intracellular concentrations of adenosine 3':5'-cyclic phosphate was used in a sensitive and convenient colorimetric assay for cholera toxin. s49 cells suspended in dulbecco's modified eagle's minimal essential medium containing 10(-5)--10(-6) m ro 20-1724, an analogue of 4-(3,4-demethoxybenzyl)-2-imidazolidinone and a phosphodiesterase inhibitor, were growth-inhibited by subnanogram concentrations of cholera t ... | 1978 | 580785 |
| nucleic acids hydrolysis by vibrio cholerae. | 1978 | 581570 | |
| antibiotic resistance of vibrio cholerae: special considerations of r-plasmids. | studies on the transmission of r plasmid by conjugation between enterobacteria and vibrio or related bacteria were reviewed. the majority of the reports confirmed successful transmission from enterobacteria to vibrio cholerae and related species, although the transmission frequencies were extremely low and the transmitted r plasmid was very unstable except for thermosensitive kanamycin plasmid and usual r plasmid coexisting with p plasmid. strains of v. cholerae and aeromonas liquefaciens as wel ... | 1978 | 581573 |
| secretory iga antibodies to enterobacterial virulence antigens: their induction and possible relevance. | 1) milk and salivary s-iga antibodies are via the homing of iga producing cells from the peyer's patches closely connected with antigenic stimuli in the intestine. this explains the presence in human milk of s-iga antibodies against e. coli o and k antigens, v. cholerae and shigella o antigens, e. coli and v. cholerae enterotoxins. these secretory antibodies can be induced by intestinal exposure and boosted by parenteral vaccination. 2) preliminary data suggest that the iga response in the urina ... | 1978 | 369311 |
| adhesion and chemotaxis as determinants of bacterial association with mucosal surfaces. | 1) chemotaxis of cholera vibrios facilitates the association of these bacteria with the mucosal surface. 2) mucosal extracts can block the chemotactic receptors on the bacterial surface and thereby retard the association of chemotactic bacteria with the mucosal surface. 3) chemotactic cholera vibrios grow more efficiently than non-chemotactic mutants in germfree mice and in isolated intestinal loops of rabbits. conversely, non-chemotactic mutants grow more efficiently in infant mice. 4) chemotax ... | 1978 | 369312 |
| [the role of interhuman contacts in the transmission of cholera, epidemics of 1832 and 1854]. | by mapping the evidence of excess mortality month by month for cholera epidemics of 1832 and 1854, which were the most deadly and most typical of their kind in france, the authors are able to provide a kinetic description of the propagation of the asian disease throughout the whole of france. traditionally it has been assumed that hydric anademia explains, for the most part, the paths taken by the disease and its varying intensity, but the importance of direct interhuman contamination is demonst ... | 1978 | 369727 |
| immunological studies on the ammonium sulphate fractions of vibrio cholerae cell lysate. | 1978 | 369995 | |
| [reactogenicity and immunological effectiveness of an oral cholera chemical vaccine in a limited controlled experiment with human revaccination]. | oral cholera chemical vaccine in the doses tested (2 and 3 tablets) proved to be areactogenic, harmless and immunologically effective in a controlled limited trial in 150 volunteers. by the results of titration of specific antitoxins and vibriocidal antibodies in the blood serum, as well as of coproantibodies a dose of 2 tablets was chosen as the optimal one. | 1978 | 371293 |
| [role of normal microflora in the treatment of vibrio carrier state in rat gnotobionts]. | materials on the study of interactions between vibrio cholera el tor and some indigenous bacteria of human intestinal tract, which proved to be active antagonists in vitro, are presented in this work. experiments were conducted on gnotobiotic rats (fisher), using various combinations and variants of the order of the microorganism administration; there were revealed no antagonistic relations between virbrio cholerae el tor and escherichia coli m-17, lactobacillus fermenti, lactobacillus plantarum ... | 1978 | 352437 |
| [suture of vessels in microsurgery (author's transl)]. | 1978 | 352482 | |
| preparation of acid-fast microscopy smears for proficiency testing and quality control. | a method is presented for preparing smears for proficiency testing and quality control in acid-fast microscopy. the work was prompted by the increased demand for acid-fast bacilli positive smears with characteristic microscopic appearance and among-smear uniformity. | 1978 | 353070 |
| effects of eustachian tube ligation on auditory nerve responses to clicks. | auditory nerve responses to condensation and rarefaction clicks were recorded from the external ear canal of cats using a closed acoustic system. repeated control recordings from both ears formed a baseline for each of four animals used in this study. after a baseline had been established, the eustachian tube on one side was ligated and serial recordings of n1 responses were performed for up to 140 days postligation. by comparing the shift which occurred in the n1 latency-vs-click level plots af ... | 1978 | 414648 |
| glycosylation of endogenous lipids and proteins by preparations of chicken embryo fibroblasts. | glycosylation of endogenous phosphoisoprenyl lipids and membrane-associated proteins was shown to occur in preparations of chicken embryo fibroblasts incubated with gdp[14c]mannose and udp-n-acetylglucosamine. the two preparations used were cells released from the culture dishes by buffered saline containing edta and crude membranes from those cells. both beta-mannosyl-phosphoryldolichol and oligosaccharide-phosphoryl lipids with five to eight sugar residues were labelled under the conditons emp ... | 1978 | 414785 |
| determination of corynebacterium diphtheriae toxigenicity by a colorimetric tissue culture assay. | chinese hamster ovary (cho) cell cultures in microtiter wells are sensitive to growth inhibition and killing by picogram quantities of diphtheria toxin. in the absence of biologically active toxin, the cho cell culture produces sufficient acidic metabolites to change the phenol red ph indicator from pink to yellow within 56 h. in the presence of 10 pg of toxin per well, growth inhibition can be observed microscopically within 24 h. diphtheria toxin can be qualitatively assayed from culture super ... | 1978 | 415068 |
| the effects of severe keratitis on corneal transmission. | an investigation into the effects of keratitis on corneal light transmission is presented. transmission data from 9 diseased corneas are compared to those of 22 normal corneas in the 0.3-0.8 micron spectral range. rhesus monkey corneas were used in the study. several color photographs illustrate varieties of idiopathic keratitis in rhesus monkeys. | 1978 | 415084 |
| respiratory syncytial virus infection in adults: clinical, virologic, and serial pulmonary function studies. | we prospectively studied 10 previously healthy adults who developed an acute respiratory illness while working in an infants' ward during a community outbreak of respiratory syncytial virus infection. in addition to clinical and viral evaluation, total respiratory resistance before and after carbachol aerosol inhalation was measured. all 10 subjects had respiratory syncytial virus infection documented by viral isolation, and all developed pronounced cough, nasal congestion, and fever. eight subj ... | 1978 | 415653 |
| connective tissue composition of aortas from non-human primates. a comparative study. | connective tissue composition of aortas from several non-human primate species has been studied in an effort to relate collagen, elastin, ang glycosaminoglycan (gag) content to species susceptibility to atherosclerosis. among the species studied the baboon contained the highest content of gag in the aorta. while the distribution of individual gag varied from species to species, heparan sulfate (hs) was the highest gag in aortas from most of the species. the ratio of hs to chondroitin sulfates (c ... | 1978 | 415748 |
| slow virus e malattie croniche del sistema nervoso centrale. | the purpose of this report was to detect whether some subacute or chronic human neurological diseases are significantly associated with traditional virus like papovavirus and paramixovirus and non conventional agents (scrapie-like) or non classified (multiple sclerosis associated agent or msaa). in this connection for m.s. the data concerning the ethiological factors paramixovirus or mssa are presented and discussed and it is hypothesized the virus mechanism in the pathogenesis of m.s. | 1978 | 415749 |
| effect of age, weight and adequacy of zinc intake on the balance between alkaline ribonuclease and ribonuclease inhibitor in various tissues of the rat. | 1. deficiency of zinc inhibits growth and also increases the activity of alkaline ribonuclease in certain tissues of the rat (prasad & oberleas, 1973). zn could influence ribonuclease activity by direct effects on the enzyme or its natural inhibitor, or non-specifically as occurs when growth rate is affected by various other factors. these possibilities were studied. 2. alkaline ribonuclease was shown to be inhibited by zn in vitro, but the concentrations of zn required were so high that the enz ... | 1978 | 415757 |
| evaluation of a surgical method for immobilizing the eye of an alert monkey. | one eye of a macaque monkey was immobilized by severing the third, fourth, and sixth cranial nerves. the residual movements of the operated eye were measured by repeatedly mapping the position of a visual receptive field. movements of several degrees were caused by contraction of the orbital musculature during the closure blink. the amount of movement was dramatically reduced by paralysis of the superficial orbital muscles with a local anesthetic. side effects of surgical immobilization include ... | 1978 | 415895 |
| ribitol and d-arabitol catabolism in escherichia coli. | in escherichia coli c, the catabolism of the pentitols ribitol and d-arabitol proceeds through separate, inducible operons, each consisting of a dehydrogenase and a kinase. the ribitol operon is induced in response to ribulose, and the d-arabitol operon is induced in response to d-arabitol. each operon is under negative control. the genes of the ribitol and d-arabitol operons are very closely linked and lie in a mirror image arrangement, rtlb-rtla-rtlc-atlc-atla-atlb, between metg and his on the ... | 1978 | 350825 |
| selective inhibition of klebsiella aerogenes growth on pentoses by pentitols. | selective inhibition of growth by pentitols was observed when klebsiella aerogenes m-7 which could not utilize pentitols was grown on pentoses. d-arabitol inhibited the growth on d-arabinose as a sole carbon source, but had no effect on the growth on l-arabinose, d-xylose, and d-ribose. similarly, l-arabitol inhibited the growth on d-arabinose and l-arabinose, ribitol inhibited the growth on d-arabinose and l-arabinose, and xylitol inhibited the growth on d-xylose. from the following reasons, we ... | 1978 | 350845 |
| analogs of the dnab gene of escherichia coli k-12 associated with conjugative r plasmids. | the dnab266(am) mutation in escherichia coli k-12 is an amber mutation such that strains carrying this mutation are not viable in a sup+ strain. with five different r plasmids, it has been possible to construct viable r+ derivatives of this amber mutant and show that the plasmids themselves do not carry amber suppressors. this is interpreted as evidence for the presence of dnab analog genes associated with these plasmids. plasmid-positive strains carrying these genes often showed some degree of ... | 1978 | 350846 |
| electron microscope heteroduplex studies of sequence relations among plasmids of escherichia coli: structure of f100, f152, and f8 and mapping of the escherichia coli chromosomal region fep-supe-gal-attlambda-uvrb. | the genetic and physical structures of commonly used f-prime factors carrying the galactose region of the escherichia coli chromosome were analyzed. deletions in the chromosomal dna sequences in the f-prime factors were found to be frequent events. a genetic method was developed to reconstruct the original f-prime factors from deletion variants. heteroduplex analysis of the reconstructed f-prime factors confirmed the derivation of the f-prime factors f100 and f152, from the same hfr, and finally ... | 1978 | 350847 |
| rich culture medium for the radiochemical labeling of proteins and nucleic acids. | yeast extract was treated with tyrosine decarboxylase and used to prepare a rich, complex medium virtually free of tyrosine. the medium supported maximal growth rates for escherichia coli prototrophs, as well as for defined and undefined auxotrophs. it has made possible the efficient radiochemical labeling of cells growing optimally in complex medium and the characterization of mutants with undefined requirements. similarly prepared media may be useful for the study of fastidious organisms and o ... | 1978 | 350856 |
| n10-formyltetrahydrofolate is the formyl donor for glycinamide ribotide transformylase in escherichia coli. | glycinamide ribotide transformylase from escherichia coli was obtained free of n5,n10-methenyltetrahydrofolate cyclohydrolase activity by deae-cellulose chromatography. in reaction mixtures containing this enzyme preparation in potassium maleate buffer, ph 7.2, no detectable interconversion of n5,n10-methenyltetrahydrofolate occurred. upon addition of glycinamide ribotide, n-formylglycinamide ribotide was formed when n10-formyltetrahydrofolate was present; no formylation occurred in the presence ... | 1978 | 350869 |
| assay method for vibrio cholerae and escherichia coli enterotoxins by automated counting of floating chinese hamster ovary cells in culture medium. | as chinese hamster ovary (cho) cells on plastic proliferate, many cells float off into the medium instead of piling up after they form a monolayer. fewer cells were floating in the medium when cho cells were incubated with cholera toxin at a concentration as low as 10 pg/ml. the toxin increased the adhesiveness of the cells forming confluent monolayers so that the floating cells accumulated on the adherent monolayers. on the basis of this finding, a simple, quantitative assay method for cholera ... | 1978 | 350897 |
| influenza as a problem in immunology. | 1978 | 351051 | |
| sequential expression of immunoglobulin on developing mouse b lymphocytes: a systematic survey that suggests a model for the generation of immunoglobulin isotype diversity. | paired immunofluorescent staining with antibodies specific for the major isotypes of mouse immunoglobulin was used to study the ontogenetic expression of diversity of cell surface immunoglobulin. the first b lymphocytes to emerge, derived from cytoplasmic igm+ precursors, express sigm exclusively. between birth and 3 days of age separate populations of sigm+ b lymphocyte acquire a second isotype: sigd, one of the subclasses of sigg, or siga. at 3 days, all splenic b lymphocytes that bear sig or ... | 1978 | 351064 |
| [affinity modification of escherichia coli ribosomes in the region of the mrna-binding center by a heptauridylate analog bearing a chemically active group at the 3' end]. | 4-(n-2-chloroethyl-n-methylamino)-benzaldehyde acetyl derivative (rcl-derivative) of hepatauridylic acid was used to localize the structure organizing the mrna-binding site of ribosomes. this derivative; like a free oligonucleotide, stimulates the binding of [14c]phenylalanyl-trna to ribosomes and effectively alkylates ribosomes, mainly the 30s subunit within the specific complex. the alkylation being completely inhibited by preincubation with polyuridylic acid, suggests that the chemical altera ... | 1978 | 351372 |
| lymphoblast transformation in rats convalescent from infection with plasmodium berghei. | 1978 | 355194 | |
| a clinical and bacteriological study of acute diarrhoea in adults. | 1978 | 355830 | |
| [analysis of the thermostability of the hybrid dna molecules of microorganisms as a means of increasing the resolution of the molecular hybridization technic]. | a comparative study of termostability of microorganisms dna was performed in order to increase the resolution of the method of molecular hybridization. molecular hybridization was carried out and the curve of hybrid dna duplexes distribution, acccording to termostability of two groups of microorganisms, related to strains echerichia coli b1 and vibrio cholerae eltor 18647, were obtained. it was determined that the form of the curves is specie specific for the microorganisms investigated but ther ... | 1978 | 355865 |
| cholera and related diarrhoeas ('turista'). | 1978 | 357986 | |
| radial passive immune hemolysis assay for detection of heat-labile enterotoxin produced by individual colonies of escherichia coli or vibrio cholerae. | a rapid screening test has been developed to detect heat-labile enterotoxin produced by individual colonies of escherichia coli or vibrio cholerae growing on media solidified with agar. the applicability of this method for isolating tox mutants of e. coli and v. cholerae has been demonstrated. | 1978 | 359590 |
| reovirus-like agent and enterotoxigenic escherichia coli infections in pediatric diarrhea in the philippines. | of 82 children hospitalized with diarrhea in the philippines during january-june 1976, 14 (17%) had infections due to a reovirus-like agent as determined by detection of viral particles in stools by electron microscopy (12 [15%] of 82) and/or by a rise in titer of antibody to the serologically related nebraska calf diarrhea virus (eight [20%] of 39). escherichia coli producing heat-labile enterotoxin were found in six (7%) of 82 ill children and two (4%) of 49 healthy control children, while e. ... | 1978 | 359725 |
| enterotoxicity of the so-called nag vibrios. | 1978 | 361004 | |
| immunological cross-reactivity between a heat-labile enterotoxin(s) of escherichia coli and subunits of vibrio cholerae enterotoxin. | heat-labile enterotoxin from escherichia coli is immunologically related to both subunits, a and b, of cholera enterotoxin as demonstrated by neutralization and immunodiffusion. | 1978 | 361573 |
| effects of injected iron and siderophores on infections in normal and immune mice. | the fate of virulent and avirulent strains of salmonella typhimurium in untreated and iron-injected mice and in transferrin-containing media demonstrated a direct relationship between bacterial virulence and the ability of bacteria to acquire transferrin-bound iron. effects of injected iron on the development of infections with virulent and avirulent bacterial strains were determined in normal and immune mice by determinations of bacterial numbers in tissue homogenates and the mortality of infec ... | 1978 | 365761 |
| failure of immunotherapy with neuraminidase-treated tumor cell vaccine in mice bearing established 3-methylcholanthrene-induced sarcomas. | c3h/hej mice bearing mc-80 fibrosarcomas were given immunotherapy consisting of multiple injections of a vibrio cholerae neuraminidase (vcn)-treated tumor cell vaccine at a site remote from the established tumor. in five separate experiments we were unable to show either partial or complete tumor regression or prolongation of survival for vaccine-treated mice compared to appropriate controls. further, the use of bcg in addition to vcn-treated tumor cells failed to show any therapeutic efficacy. ... | 1978 | 621758 |
| quantitative measurements of cholera enterotoxin in cultures of toxinogenic wild-type and nontoxinogenic mutant strains of vibrio cholerae by using a sensitive and specific reversed passive hemagglutination assay for cholera enerotoxin. | a sensitive and specific reversed passive hemagglutination (rpha) assay for cholera enterotoxin has been developed. equine anti-choleragenoid antibodies purified by immunoadsorption were covalently coupled to formalinized sheep erythrocytes, using bis-diazotized benzidine, and the antitoxin-sensitized erythrocytes were shown to agglutinate specifically in the presence of cholera enterotoxin. in a microtiter rpha assay system, the smallest quantity of enterotoxin that caused hemagglutination was ... | 1978 | 624584 |
| suppression of pathogenicity by p and v plasmids in vibrio cholerae. | in experimental models of cholera, some strains of vibrio cholerae harbouring p or v or both plasmids were markedly less pathogenic than isogenic strains devoid of these plasmids. this effect was due to decreased production of toxin by p+, v+ or p+v+ bacteria compared with their parents. | 1978 | 632803 |
| effect of trimethoprim-sulphamethoxazole on vibrio clearance in cholera (el tor): a comparative study. | the efficacy of trimethoprim-sulphamethoxazole (tmp-smx) has been compared with that of tetracycline and chloramphenicol in 175 bacteriologically confirmed cases of cholera admitted to the infectious diseases hospital delhi. vibrio cholerae, biotype el tor, serotype ogawa, were isolated from all the patients. tmp-smx showed greater in vitro inhibition and earlier eradication from the intestinal tract and is recommended as a suitable vibriocidal agent against cholera. | 1978 | 635974 |
| on the significance of sialic acid in high affinity 5-hydroxytryptamine uptake by synaptosomes. | synaptosomes isolated from rat brain cortex incorporated [14c]5-hydroxytryptamine at 37 degrees c with high affinity. an apparent transport constant of kt = 50nm was found. the high affinity uptake was decreased by treatment of synaptosomes with neuraminidase from vibrio cholerae or clostridium perfringens prior to incubation with [14c]5-hydroxytryptamine. the inhibition was related to the amount of sialic acid released, with a ki value of 3.5 micrometer. a non-competitive type of inhibition was ... | 1978 | 640586 |
| some interesting isolates from a diagnostic laboratory. | citrobacter koseri, plesiomonas shigelloides, edwardsiella tarda, yersinia enterocolitica, alkalescens dispar, vibrio parahaemolyticus, and vibrio alginolyticus were seven interesting microorganisms isolated recently in our diagnostic laboratory. | 1978 | 641196 |
| non-cholera vibrio infections in the united states. clinical, epidemiologic, and laboratory features. | non-cholera vibrios are organisms that are biochemically indistinguishable from vibrio cholerae but do not agglutinate in vibrio 0 group 1 antiserum. since 1972 there has been a dramatic increase in the number of these organisms referred to the center for disease control for identification. clinical, epidemiologic, and laboratory data were analyzed for 26 of 28 patients with isolates identified between january 1972 and march 1975. thirteen (50%) of the isolates were obtained from feces of patien ... | 1978 | 646241 |
| [morphological and immunomorphological changes in the intestines and regional lymph nodes of gnotobiotic rats--chronic carriers of vibrio cholerae el-tor]. | in the course of chronic carrier state of el-tor vibrio cholerae gnotobiotic rats develop an immunomorphological reaction in the mucous membranes of the small and large intestines, peter's patches, and mesenterial lymph nodes consisting in activation of reactive centers of follicles, hyperplasia of reticular cells, and an increase in the number of pyroninophilic plasma cells. the vibrio cholerae caused no pathological changes in enterocytes and other cells but its inoculation was accompanied by ... | 1978 | 646664 |
| [a patient with cholera in the netherlands]. | 1978 | 652083 | |
| the critical role of iron in host-bacterial interactions. | the ability of potential pathogens to acquire iron in a host is an important determinant of both their virulence and the nature of the infection produced. virulent gram-negative bacteria are capable of acquiring sufficient iron from the host because their virulence (for chick embryos) is unaffected by exogenous iron. avirulent mutants which are apparently limited in their ability to acquire iron could be isolated from the virulent strains. the lethality of these mutants was significantly enhance ... | 1978 | 659605 |
| [selected chapters from clinical bacteriology. ii. cholera]. | 1978 | 662828 | |
| [enteropathogenicity of hemolysing el tor vibrios]. | the authors studied the enteropathogenic properties of 11 strains of hemolysing e1 tor vibrios, of which 8 in enteric administration to suckling rabbits caused no death of the animals, and 3 caused the animal death with the phenomena of diarrhea, but without any typical cholerogenicity syndrome. in case of administration with mucine the pathogenic properties were revealed in 6 strains more. use of strains grown on media with starch for the infection led, in individual cases, to the manifestation ... | 1978 | 665017 |
| [effect of sulfide water from natural springs on the properties of vibrino cholerae]. | the authors studied the properties of e1 tor cholera vibrios isolated from sulfide water of the natural sources. there was demonstrated under experimental and natural conditions the influence of ecological conditions of sulfide water on such vibrio properties as cholerogenicity, sensitivity to diagnostic and typing phages, hemolytic activity and the value of the hemolysin-destructive factor. a short-liver stay of cholera vibrios in sulfide water was accompanied by some reduction of their virulen ... | 1978 | 665018 |
| [el tor vibrios with different enteropathogenicities]. | the authors summed up the results of study of enteropathogenicity of 270 strains of the el tor vibrios isolated the last 11 years. over 2 000 nursling rabbits were used. el tor vibrios were divided by their enteropathogenic properties into three categories: 1) the highly enteropathogenic (cholerogenic) ones causing the death of all the biotest nursling rabbits with a characteristic syndrome of cholerogenicity, and failing to lyse sheep erythrocytes; 2) enteropathogenic ones, causing death of som ... | 1978 | 665027 |
| [search for an optimal medium for culturing the cholera vibrio using a method of mathematical experimental design]. | 1978 | 665047 | |
| neuraminidase gangliosides interactions. | 1978 | 665377 | |
| purification of cholera toxin and its subunits: new methods of preparation and the use of hypertoxinogenic mutants. | cholera toxin was obtained in pure form by fractionation on two phosphocellulose columns successively. cholera toxin and choleragenoid were quantitatively and selectively adsorbed to the first column in 10 mm phosphate buffer, ph 7.0, and were subsequently eluted with buffer of high ionic strength. the toxin was then separated from choleragenoid on the second column by chromatography at ph 8.3. the toxin obtained was highly active and pure as judged by electrophoresis, isoelectric focusing, and ... | 1978 | 669812 |
| sialic acid release by vibrio cholerae sialidase (neuraminidase) from the outer surface of neural cells is greatly raised by halothane. | 1978 | 671010 | |
| [the choice of optimal sensitizing dose of the antigen in the preparation of cholera erythrocytic diagnostic agent, with consideration to the avidity of antibodies under study]. | the authors studied the comparative antigenic activity in the indirect hemagglutination test (ihat) of erythrocytic diagnostic agents sensitized with various doses of cholera o-antigen. a definite dependence of the ihat results on the avidity degree of the antibodies determined in the serum was demonstrated. to ascertain the maximal levels of highly avid antibodies (in the hyperimmune cholera sera) it is possible to use antigen doses exceeding the optimal sensitizing ones. in examination of less ... | 1978 | 676601 |
| structural studies of a specific polysaccharide isolated from non-agglutinable vibrio. | the purified, specific polysaccharide from vibrio cholera type nag, nv 384, o-antigen, 2a, 2b human, contains glucose (5.14%), galactose (4.21%), mannose (64.8%), xylose (3.16%), arabinose (1.98%), fucose (1.50%), mannuronic acid (14.3%), phosphate (0.32%), 2-amino-2-deoxy-d-glucose (2.9%), and 2-amino-2-deoxy-d-galactose (1.0%). various reactions have shown that the material comprises a phosphoric diester-linked polysaccharide containing mainly ( 1 leads to 2)-linked mannopyranose residues that ... | 1978 | 679219 |
| isolation and characterization of protease-deficient mutants of vibrio cholerae. | mutants of vibrio cholerae that were deficient in protease production were isolated by picking clones form gelatin or casein plates which showed reduced zones of proteolysis. all mutants showed reduced ability to degrade complex proteins (casein and gelatin), and those tested were deficient in ability to degrade chicken egg ovomucin. some of the mutants demonstrated a decrease in neuraminidase activity. almost all mutants showed a dramatic loss of virulence in the infant mouse, although toxin wa ... | 1978 | 681794 |
| [isolation and properties of cholera vibrio neuraminidase]. | 1978 | 685200 | |
| vibriocidal antibody titer rise after rectal or anal administration of vibrio cholerae in dogs. | in many developing countries and ablution with surface water is a daily habit. this water is heavily contaminated with microbes, raising the possibility that and contamination may lead to immune responses. this hypothesis was experimentally tested in 21 dogs by anal or rectal application of vibrio cholerae suspension v. cholerae was recovered from rectal swab cultures for a mean of one day after rectal contamination and 2.5 days after anal application (p less than .02). significant increases occ ... | 1978 | 685952 |
| the 4-amino sugars present in the lipopolysaccharides of vibro cholerae and related vibrios. | brief treatment, with 10 m hydrochloric acid, of the lipopolysaccharides of vibrio cholerae and related organisms led to the release of 4-amino-4, 6-dideoxy-d-mannose (d-perosamine) and 4-amino-4-deoxyl-l-arabinose. these sugar are highly unstable and undergo complex changes in neutral and basic media. of the strains examined, the lipopolysaccharides from representatives of the inaba group contain only d-perosamine, while those of the ogawa biotype have 4-amino-4-deoxyl-l-arabinose as an additio ... | 1978 | 687663 |
| effect of immunization by bcg and phytohaemagglutinin-p on the kinetics of antibody response to v. cholerae. | 1978 | 689711 | |
| intestinal distribution of vibrio cholerae in orally infected infant mice: kinetics of recovery of radiolabel and viable cells. | kinetics of distribution of vibrio cholerae in the gastrointestinal tract of orally challenged infant mice were examined by determining recovery of input dose from the whole gut and from individual segments of stomach, upper bowel, and lower bowel. the strains studied were 569b, ca401, and vb12 (a rough ca401). recovery was determined as a percentage of either input radiolabel using 35s-labeled cells or input colony-forming units. we found clearance of radiolabel and viable cells from the stomac ... | 1978 | 689734 |
| mannose-sensitive haemagglutinins in adherence of vibrio cholerae eltor to intestine. | 1978 | 690600 | |
| protective antitoxic cholera immunity in mice: influence of route and number of immunizations and mode of action of protective antibodies. | an adult mouse model has been elaborated for studies of experimental cholera (vibrio cholerae enterotoxin-induced intestinal secretion) and protective antitoxic immunity in either ligated small bowel loops or intact small intestine. mice of different inbred strains varied markedly in intestinal susceptibility to toxin, c57b1 being the most sensitive strain tested. fluid accumulation started within 1 h after the inoculation of toxin and was maximal after 5 h, whereafter recovery gradually ensued. ... | 1978 | 696335 |
| stability of r plasmids belonging to different incompatibility groups in vibrio cholerae "eltor". | twenty two resistance plasmids belonging to fourteen incompatibility groups have been transferred by conjugation into two sensitive strains of vibrio cholerae "eltor". only five plasmids were stably inherited in these strains: four of these belong to group incc, and one to group incj. all other plasmids were lost at high frequency when the bacteria were grown in drug-free medium. these facts might explain why very few multiresistant strains of v. cholerae have been isolated so far but do not exc ... | 1978 | 697261 |
| genetic transformation of enterobacteria & vibrios to streptomycin resistance. | 1978 | 700786 | |
| vibrio cholerae neuraminidase susceptible n-acetyl neuraminic acid (sialic acid) levels in human leukaemia. | 1978 | 700974 | |
| single-dose doxycycline for cholera. | to determine the efficacy of single-dose doxycycline in the treatment of cholera, we carried out a randomized prospective trial in 65 patients. treatment consisted of either a single dose of 200 mg of doxycycline (or 4 mg/kg in patients less than 15 years old) or multiple doses of doxycycline, 500 mg over 4 days (or 10 mg/kg in patients less than 15 years old). there were no differences between the groups in the volumes of intravenous fluid required, volumes of diarrheal stool, or durations of d ... | 1978 | 708024 |
| induction of a mucosal antitoxin response and its role in immunity to experimental canine cholera. | the induction of a jejunal antitoxin response was studied in dogs immunized with cholera toxin or toxoid. single doses of toxoid given subcutaneously (s.c.) or of toxin given intraluminally (i.l.) were each effective in priming the mucosal immune system, whereas toxoid given i.l. was much less effective. in contrast, toxin and toxoid given i.l. were each effective as booster antigens. the local secondary response was rapid and brief, the peak occurring at about 7 days after i.l. boosting and dec ... | 1978 | 711314 |
| genetic mapping of mutations in independently isolated nontoxinogenic mutants of vibrio cholerae. | conjugal mating experiments were performed between donor strains of vibrio cholerae carrying the vibrio sex plasmid p and recipient strains lacking the p plasmid. donor and recipient genotypes differed with respect to toxinogenicity (tox), nutritional requirements, and antibiotic susceptibilities. recombinants carrying selected donor and recipient markers were produced at low frequencies in conjugal matings. mapping of tox markers was accomplished by scoring for the frequency of coinheritance of ... | 1978 | 711315 |
| prevalence of non-cholera vibrios in cavum nasi and pharynx of ducks. | investigations among ducks on ten different farms showed a high prevalence of vibrio cholerae in cavum nasi and pharynx after the ducks were admitted to the open field. in no case was vibrio cholerae isolated from ducks which had never been outside the houses. at least six serovars were isolated, 0:54 being the most prevalent. all the strains isolated produced a distinctive cytotoxic effect in yi adrenal cells and caused fluid accumulation in rabbit gut loops. migratory birds were incriminated a ... | 1978 | 716918 |
| [animal study on radioactive antigenic fractions isolated from v.cholerae. i. the ch 1 + 2 fraction]. | the ch 1 + 2 fraction has been marked by means of culture of vibrio cholerae ogawa hk1 on a synthetic medium containing leucine h3. the antigen distribution has been then studied before and after vaccination with the same non-marked antigen in normal and axenic mice, at the same time by the demonstration of radioactivity and radioimmunofluorescence in intestine, spleen, liver, kidney and thymus. whichever the administration route, intestine and spleen are first stimulated, then more intensively ... | 1978 | 719845 |
| desialylation of glycoconjugates using immobilized vibrio cholerae neuraminidase. preparation, properties and use of the bound enzyme. | neuraminidase from vibrio cholerae was immobilized on sepharose 4b using the cyanogen bromide technique. the properties of the bound enzyme were found to be similar to those of the soluble form, except for an appreciably improved stability on storage at equivalent dilution and a reduction in recovered activity. evidence was obtained that the binding of large molecular weight substrates to the bound enzyme is modified due to the immobilized state of the enzyme. the use of the enzyme gel for desia ... | 1978 | 721069 |
| [morphologic variability of cholera vibrios]. | 1978 | 726740 | |
| [viability and properties of cholera vibrios cultivated in mineral water]. | samples of mineral water from thermal spas of "matsesta" health resorts were infected with cholera vibrio strains. in nonsterile mineral water diluted with distilled water 1 : 2, 1 : 4, and 1 : 8 and stored at 37 degrees c survival periods of cholera vibrio constituted 289 days for classic biotype, and 413 days for el tor biotype. in boiled mineral water stored at 20-24 degrees c this period was 1429 days for both biotypes. prolonged cultivation of cholera vibrios in mineral water had no effect ... | 1978 | 726747 |
| glycine-containing lipopolysaccharide isolated from vibrio cholerae 4715 (nag). | 1978 | 729130 | |
| study of lactose teepol agar in diagnosis of cholera. | 1978 | 730265 | |
| isolation and phenotypic characterization of virulence-deficient mutants of vibrio cholerae. | mutants of vibrio cholerae was isolated on the basis of reduced ability to induce diarrhea in orally challenged infant mice. nitrosoguanidine-treated clones were screened for low fluid accumulation ratios in individual mice, and presumptive mutants were confirmed in additional mouse tests. mutants were examined for alterations in phage type, motility, toxin production, proteolytic activity, neuraminidase production, amylase production, morphology, growth requirements, carbohydrate fermentations, ... | 1978 | 730346 |
| role of motility in experimental cholera in adult rabbits. | the role of motility in the pathogenesis of cholera was evaluated in ligated ileal loops of adult rabbits. four strains of vibrio cholerae (including both inaba and ogawa serotypes of both classical and el tor biotypes) were compared with their aflagellated, but fully toxigenic and prototrophic, isogenic derivatives as to their ability to produce fluid accumulation in the rabbit gut. the nonmotile mutants required an at least 100-fold-higher dose than their respective wild-type strains to produc ... | 1978 | 730361 |
| survival and multiplication of vibrio cholerae in the upper bowel of infant mice. | the survival and multiplication of vibrio cholerae strains of varying virulence in the upper bowel of orally challenged infant mice early in infection has been examined. analysis of changes in the apparent specific activity (counts per minute per colony-forming unit) of the cell population after 4 h compared with the inoculum indicated that strain ca401 established a viable, multiplying cell population, whereas strains vb12 (a rough variant) and 569b were subject to host bactericidal and bacteri ... | 1978 | 730364 |
| cholera: possible infection from aircraft effluent. | this paper presents the hypothesis that some cases of cholera might be due to effluent discharge from aircraft. the theoretical case is borne out by inspection of data on the physical conditions pertaining between high altitudes and ground level. a study of the distribution of isolated outbreaks and single cases of disease and their relation to major airline routes showed a reasonable correspondence. sporadic outbreaks of cholera in europe between 1970 and 1975 were found to lie within the fligh ... | 1978 | 731020 |
| sialic acid: a specific role in hematopoietic spleen colony formation. | vibrio cholerae neuraminidase (vcn) treatment of donor bone marrow cells results in a reduction in the number of hematopoietic colonies (cfus) formed in the spleens of lethally irradiated mice. treatment of marrow cells with sodium periodate under mild conditions, known to preferentially oxidize sialic acid, also reduced cfus while subsequent potassium borohydride reduction restored cfus to 80% of control levels. innoculum viability as measured by in vitro incorporation of tritiated precursors i ... | 1978 | 732308 |
| plasmid-induced loss of virulence in vibrio cholerae. | 1978 | 732874 | |
| [occurrence and ecology of nag vibrios in surface waters (author's transl)]. | 1978 | 735570 |