Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [study of immunologic parameters in parenteral drug addicts (pda) with condylomata acuminata]. | a study is made of the state of cell and humoral immunity in parenteral drug addicts both with and without genital warts. the results reveal alterations in the lymphocyte subpopulations, with a decrease in the t-helper/t-suppressor (t4+/t8+) ratio of both groups of patients. these alterations were more pronounced in those patients with genital warts (p less than 0.05). the alterations in humoral immunity, expressed as a hypergammaglobulinemia igg and igm, were similar in both groups. none of the ... | 1988 | 2904516 |
| impaired ability of t4+ lymphocytes in the active stage of congenital hypoplastic anemia to promote in vitro growth of blood erythroid burst-forming units (bfu-e). | 1988 | 2904723 | |
| histamine-induced regulation of il-2 synthesis in man: characterization of two pathways of inhibition. | histamine at molar concentrations ranging from 10(-5) to 10(-7) exerted an inhibitory effect upon il-2 synthesis by peripheral blood mononuclear cells in normal man; two pathways of inhibition are described. the first pathway was found to alter the t4 lymphocytes which, in the system used in this study, synthesized nearly 90% of the total il-2 production and had no suppressive activity. this suggests that histamine can act at the level of il-2-producing cells. the second pathway of inhibition wa ... | 1988 | 2905136 |
| [aids. excessive decrease in t4 lymphocytes]. | 1988 | 2905322 | |
| a randomized placebo-controlled trial of recombinant human interferon alpha 2a in patients with aids. interferon alpha study group. | we performed a randomized, double-blind, placebo-controlled trial to assess the tolerance and efficacy of recombinant human interferon alpha 2a (roferon a) in patients with acquired immunodeficiency syndrome (aids) without kaposi's sarcoma. a total of 67 patients were enrolled in five medical centers from october 1983 through april 1986, and received either placebo, 3 million units, or 36 million units of interferon alpha three times a week for 12 weeks. there were no significant differences in ... | 1988 | 2905740 |
| cellular immunity and the hypothalamic-pituitary axis in major affective disorder: a preliminary study. | to assess cellular immune status and the hypothalamic-pituitary axis in patients with major depression, we examined peripheral blood mononuclear cells and measured the plasma levels of four neurohormones. eleven patients with major depression had increased % of t4 lymphocytes and decreased concanavalin (con a) stimulated t lymphocyte proliferation when compared with 11 age-, sex-, and race-matched control subjects. percent of total lymphocytes labeled as all t lymphocytes, all b lymphocytes, and ... | 1988 | 2905815 |
| [prognostic significance of the t4 lymphocyte count in patients infected with cdc class ii and iii hiv]. | from 1985 to 1987 101 patients infected with the human immunodeficiency virus (hiv) were seen at our outpatient clinic and prospectively analyzed in a cohort study. we evaluated the follow-up in 41 patients of cdc classes ii and iii with regard to the number of t-helper lymphocytes (t4) and clinical progression. the patients consisted of 24 i.v. drug addicts, 11 homosexuals and 6 persons with other or multiple risk factors for hiv infection. patients with a t4 count of less than 400/mm3 at the f ... | 1988 | 2905830 |
| mutagenicity of topoisomerase-active agents in bacteriophage t4. | recently, the antitumor agent 4'-(9-acridinylamino)-methanesulfon-m-anisidide (m-amsa) was shown to revert a frameshift mutant of t4 (rfc11), and its mutagenicity was shown to be mediated by t4 dna topoisomerase ii [ripley et al.: j mol biol 200: 665-680, 1988]. here we report dose-response data on the mutagenicity and toxicity of m-amsa in t4 rfc11. we find that m-amsa is among the most potent frameshift mutagens observed in t4, inducing a 10-fold increase in mutant frequency in the absence of ... | 1988 | 2905838 |
| [electron microscopic immunohistochemistry in the diagnosis of human lymphotropic viral diseases]. | immunohistochemistry is finding an ever increasing application for electron microscopic diagnosis of human viral diseases. certain progress has been made in the use of peroxidase-dab/oso4 as an electron-dense marker, and colloid gold. the paper discusses immunohistochemistry application for electron microscopic detection of such lymphotropic viruses as vsv (virus of vesicular stomatitis cultured in mink's lung cells), htlv-1 (virus of human t4-cell c91pl lymphoma culture) and htlv-iii/hiv-1 (vir ... | 1988 | 2905847 |
| lymphocytes subset in hyperandrogenic women with polycystic ovarian disease. | the correlation between the sex steroids and the immune system is already known but the relationships between testosterone, progesterone, estradiol, cortisol and the immune system are still controversial. the authors have studied lymphocytes subset in ten women with polycystic ovarian disease (pcod); it was observed: 1) a decrease of t8 in 64% and of natural killer activity (nk) in 60% of patients, and an increase of t4/t8 ratio in 55%; 2) a significant negative correlation between androstenedio ... | 1988 | 2906494 |
| [cell membrane changes and skin diseases]. | biological membranes are laminar bilayers of lipoids with thermolabile biophysical properties. changing temperatures not only result in altered polarity and permeability of the membranes, but also in changes of the various membrane receptors (e.g. histamine and beta-adrenergic receptors) and the cell makers. in healthy persons, lower temperatures (25 degrees) lead to elevated suppressor properties, and the t4 ratio is below 1.0. at 37 and 41 degrees c, there is a growing number of helper cells, ... | 1988 | 2907212 |
| [effect of various beta-adrenolytic drugs and calcium channel blockers (nifedipine) on serum levels of t3 and t4 in patients with hyperthyroidism and simple goiter]. | 1988 | 2907475 | |
| hematologic and immunologic changes in hairy cell leukemia patients treated with alpha interferon. | we prospectively evaluated hematologic and immunologic changes in fourteen hairy cell leukemia patients treated with alpha interferon. prompt disappearance of circulating hairy cells, and recovery of peripheral blood counts was observed in all patients whereas only four patients had complete disappearance of hairy cell infiltrates from their bone marrow. immunologic changes included a statistically significant increase of nk cell activity in the whole group and a slight decrease in circulating t ... | 1988 | 2907963 |
| monocyte defect causes decreased automlr in multiple sclerosis patients. | we have recently demonstrated that peripheral blood monocytes from patients with multiple sclerosis (ms) have a defect in stimulating autologous and allogeneic t lymphocytes. this defect was found to correlate with disease activity. we report here that t4+ cells from ms patients proliferate weakly in the autologous mixed lymphocyte reaction (automlr). furthermore, we provide direct proof that the depressed t4+ cell proliferation is due to the monocyte functional (stimulatory) defect. | 1988 | 2908171 |
| [comparison of the action kinetics of alcuronium and vecuronium by electromyographic monitoring]. | twenty-eight asa i or asa ii adults undergoing microsurgery were anaesthetized according to a standard protocol using droperidol, phenoperidine and thiopentone followed by enflurane. the patients were randomly assigned to two homogeneous groups: the first group (n = 14) received 0.2 mg.kg-1 alcuronium, whereas the second group (n = 14) received 0.08 mg.kg-1 vecuronium. there was no reinjection of either drug and curarization tapered off spontaneously. neuromuscular monitoring was begun once anae ... | 1988 | 2894789 |
| defective expression of the 2h4 molecule after autologous mixed lymphocyte reaction activation in systemic lupus erythematosus patients. | previous studies demonstrated that patients with active systemic lupus erythematosus (sle), especially those with active renal disease, had a marked reduction in t4+2h4+ suppressor inducer cells in their peripheral blood. however, it was puzzling to find that active sle patients without renal diseases often had normal percentages of t4+2h4+ cells. in the present study, we attempted to determine whether active sle patients bearing normal percentages of t4+2h4+ cells had a defect in their expressi ... | 1988 | 2971675 |
| packaging and transduction of non-t3 dna by bacteriophage t3. | a defined in vitro system for packaging t3 dna also packaged other linear dnas, including t4, lambda, and plasmid dnas. the packaging capacity was determined to be 40 kb (kilobase pairs) by measuring the packaged length of t4 dna. packaged lambda and plasmid dnas were injected into host cells to form plaques and transductants, respectively. the yield of transducers increased by using artificially ligated plasmid oligomers. the t3 mutant in gene 3 endonuclease (t3 3-) packaged plasmid dna during ... | 1988 | 2972112 |
| distinct phenotypic composition of diffuse interstitial and perivascular focal infiltrates in renal allografts: a morphometric analysis of cellular infiltration under conventional immunosuppressive therapy and under cyclosporine a. | phenotypic analysis of interstitial mononuclear cell infiltrates was undertaken in 40 transplant renal specimens obtained from 38 patients in order to assess the influence of immunosuppressive therapy. thirteen patients were given conventional immunosuppressive treatment (azathioprine and prednisone) and the other 25 received cyclosporine. the immunostaining was performed using seven antileucocyte antibodies by alkaline phosphatase-anti-alkaline phosphatase method. interstitial infiltrates were ... | 1988 | 2972428 |
| human thymic dendritic cells. characterization, isolation and functional assays. | the phenotypic analysis of human thymic dentritic cells (dc) in culture and in purified suspensions has been studied with light and electron microscopic (em) immunolabelling techniques. using a series of monoclonal antibodies (mab) and a protein a-gold technique, we demonstrated that dr- and t6-positive cultured dc strongly bind the 9.3f10 mab, an anti-dr-related antibody produced against human blood dc, and weakly express the t4 antigen, a membrane marker shared by langerhan's cells (lc). on th ... | 1988 | 2972601 |
| dendritic cell-lymphocyte clusters that form spontaneously in rheumatoid arthritis synovial effusions differ from clusters formed in human mixed leukocyte reactions. | lymphocytes cluster about dendritic cells (dc) spontaneously in 48 h cultures of rheumatoid arthritis synovial fluid (ra sf) mononuclear cells and in peripheral blood autologous or allogeneic mixed leukocyte reactions. in the latter case, the clusters are predominantly cd4+ t cells (t4/t8 greater than 5) and with time progress in blastic cells that express il-2 (tac) and/or transferrin (t9) receptors. in contrast, the clusters in ra sf cultures have a t4/t8 ratio of less than 1 and a majority of ... | 1988 | 2972742 |
| t4+ (helper phenotype) chronic lymphocytic leukemia: case report with liver failure and literature review. | t-cell lymphomas and leukemias are a heterogeneous group of neoplasms found in each anatomic compartment of the t system (marrow, thymus, and various peripheral tissues) and that have varying phenotypic expressions. histopathologic features of the thymic and peripheral t neoplasms do not fit into a clearly defined pattern, and clinical expressions of t neoplasms are likewise variable. this report describes a 60-year-old man with "chronic" lymphocytic leukemia of t4+ (helper) phenotype. rapid det ... | 1988 | 2972899 |
| presence of t-cytotoxic suppressor and leucocyte common antigen positive cells in alzheimer's disease brain tissue. | cells expressing leucocyte common antigen (lca) as well as t-cytotoxic-suppressor (t8) and t-helper-inducer (t4) antigens were detected in significant numbers in the hippocampus and temporal cortex of alzheimer but not normal brain tissue. leucocytes and reactive microglia strongly expressed lca, as well as the class ii major histocompatibility complex (mch) glycoprotein hla-dr, but astrocytes stained negatively. such staining indicates the immunocompetent status of microglia, as well as their p ... | 1988 | 2972943 |
| [hormonal determination of the differentiation of striated skeletal muscle in urodele amphibians]. | in the urodelan amphibian pleurodeles waltlii, spontaneous external metamorphosis was correlated with an increase in the serum level of thyroxine (t4). within the same period, a change occurred in the myofibrillar atpase profile of the dorsal skeletal muscle; fibres of larval type were gradually replaced by transitional fibres (type iic), then by adult fibres of the types i, iia, and iib. likewise, a myosin isoenzymic transition was observed. in larval animals, myosin electrophoresis revealed 3 ... | 1988 | 2973102 |
| formation of d loops by the uvsx protein of t4 bacteriophage: a comparison of the reaction catalyzed in the presence or absence of gene 32 protein. | the uvsx protein of t4 bacteriophage will catalyze the formation of d loops between linear single-stranded dna (ssdna) and homologous supercoiled double-stranded dna (dsdna) in the absence of t4 gene 32 protein (gp32). this reaction requires one monomer of uvsx protein per three nucleotides of ssdna so that the ssdna is completely covered with uvsx protein. under these conditions, high rates of atp hydrolysis are observed, and one-third of the products are joined paranemically. the reaction proc ... | 1988 | 2973808 |
| preferential formation of triiodothyronine residues in newly synthesized [14c]tyrosine-labeled thyroglobulin molecules in follicles reconstructed in a suspension culture of hog thyroid cells. | early processes of thyroid hormone (t4 and t3) synthesis in thyroglobulin molecules were studied using follicles reconstructed in a primary culture of hog thyroid cells under the influence of tsh. when the reconstructed follicles were incubated with 14c-tyrosine, thyroglobulin containing the labeled tyrosine was newly synthesized and in the presence of iodide, some of the labeled tyrosine residues were iodinated and coupled to produce labeled iodothyronines, t4 and t3. coupling efficiency, espec ... | 1988 | 2974002 |
| regulation of two nested proteins from gene 49 (recombination endonuclease vii) and of a lambda rexa-like protein of bacteriophage t4. | phage t4 gene 49, encoding recombination endonuclease vii, specifies, by initiation from an aug and an internal gug codon, two in-frame overlapping peptides (of 18 and 12 kd). the gene is transcribed early and late, albeit from different promoters. the sequence predicts that in long early transcripts, initiated far upstream of the coding sequence, the shine-dalgarno sequence of the first ribosome binding site can be sequestered in a hairpin and/or cleaved. these processes might reduce initiation ... | 1988 | 2974005 |
| crystallization and preliminary x-ray studies of t4 phage beta-glucosyltransferase. | crystals of the dna glucosylating enzyme beta-glucosyltransferase from phage t4 have been grown in the presence of uridine diphosphate glucose. the crystals are orthorhombic, space group p2(1)2(1)2 with a = 148.3 a, b = 52.6 a, c = 52.6 a. the assumption of one monomer of mr 40,000 per asymmetric unit gives rise to a vm of 2.56 a 3/dalton. the crystals diffract to beyond 2.7 a and are suitable for x-ray structure analysis. | 1988 | 2974086 |
| plasma atrial natriuretic peptide in states of altered thyroid function. | to examine a possible role of atrial natriuretic peptide (anp) in water and electrolyte disturbances associated with thyroid disorders, plasma anp levels were studied in patients with hyper- and hypothyroidism. in 5 of the 21 hyperthyroid patients, including two patients with atrial fibrillation and two patients with mild cardiomegaly, the plasma anp concentration was increased when compared to normal subjects. after treatment with methimazole or propylthiouracil, the plasma anp concentration fe ... | 1988 | 2974798 |
| protein content and thyroid hormone release in vitro by differentiated thyroid cancer cells in the presence of estradiol, dehydroepiandrosterone, polypeptidic hormones and pesticides. | the reactivity of thyroid cancer cells treated with estradiol (e) and dehydroepiandrosterone (dha) in the presence of other hormones (tsh, sth, insulin), myopeptide and some pesticides (aminotriazole--ata, atrazine--atz, prometryne--p and atc) proved to be active in the culture medium. protein synthesis in sclerosing folliculo-papillary cancer cells under the treatment applied is more markedly inhibited by dha than by e in the culture medium is compared to the control lots. tg1 and t3 release in ... | 1988 | 2975038 |
| oxygen diffusion distance in thyroxine-induced hypertrophic rabbit myocardium. | we studied the oxygen diffusion distance in the rabbit myocardium in untreated animals and in animals treated with thyroxine (t4) for 3 days and 16 days. the subepicardial and subendocardial regions were studied separately. sixteen days of t4 treatment results in significant cardiac hypertrophy. after 16 days, the percentage of the myocardium occupied by myocytes decreases as the volume of extracellular matrix increases. after 3 days, the number of myocyte mitochondria per unit volume of myocard ... | 1988 | 2975340 |
| [surface phenotypes of peripheral blood mononuclear cells in multiple sclerosis]. | using monoclonal antibodies the subpopulations of mononuclear cells were studied in peripheral blood in patients with multiple sclerosis in active phase of the disease. the results suggested the following conclusions: 1. in the active phase of multiple sclerosis (during exacerbations and slowly progressive course) a rise occurs in the proportions of ia-positive and m1-positive cells without changes in the proportions of t4 and t8 lymphocytes and in their mutual ratio. 2. no differences were obse ... | 1988 | 2975766 |
| [thyroid function and trisomy 21. tsh increase and rt3 deficiency]. | an excess of thyrotropin (tsh) with normal levels of tetraiodothyronine (t4) and of 3,5,3'-triiodothyronine (t3) was confirmed in the serum of 78 trisomy 21 children. a severe deficiency of 3,3',5'-triiodo-thyronine (rt3 or reverse t3) was observed and the decrease of the rt3/tsh ratio was highly significant. these new facts suggest that the rt3 deficiency plays a peculiar role in trisomy 21 (maybe through the regulation of one or few steps of monocarbons' metabolism). a systematic control of th ... | 1988 | 2975939 |
| immunophenotype of pulmonary cellular infiltrates in sheep with visceral caseous lymphadenitis. | pulmonary lesions associated with corynebacterium ovis were analyzed with an indirect immunoperoxidase staining technique using monoclonal antibodies. the predominant cells in abscess walls and surrounding lung parenchyma were large macrophages which expressed major histocompatibility complex (mhc) class ii molecules on their surfaces. t lymphocytes were prominent in the same sites in the naturally occurring lesions, and sbu-t4-positive ("helper/inducer") cells were the major subset of lymphocyt ... | 1988 | 2976546 |
| [dynamics of interleukin 2 production in patients with diffuse toxic goiter under the influence of treatment]. | the paper is devoted to the results of a study of il-2 production by t-helpers in the course of treatment of patients with diffuse toxic goiter (dtg); 30 patients and 30 healthy controls were investigated. the diagnosis of dtg was confirmed by modern methods including radioimmunoassays. a study of the blood level (relative and absolute) of t-lymphocytes in the patients before therapy, during 2 and 12 mos. of thimazol therapy and during remission of disease showed its significant decrease as comp ... | 1988 | 2976937 |
| neuroimmunomodulation in cancer patients: correlations between melatonin and beta-endorphin blood levels and t helper/suppressor ratio. | the pineal gland and opioid peptides play roles in the neuroendocrine control of immunity. both neuroendocrine and immune dysfunctions have been observed in cancer but the importance of the altered secretion of neurohormones in the immunoincompetence of cancer patients has never been investigated. this study concomitantly evaluated neuroendocrine and immune functions in 40 patients with early or advanced neoplastic disease. in each patient, melatonin and beta-endorphin blood levels and lymphocyt ... | 1988 | 2977397 |
| [hormone content of the blood in neurotic patients]. | radioimmunoassay was applied to study the hormones (corticotropin, cortisol, t3, t4, testosterone, prolactin, follicle-stimulating and luteinizing hormones) in 52 male neurotic patients and 120 healthy donors in arkhangelsk city, as compared to latitudinal mean values. in patients, prolactin, cortisol, thyroid hormones and gonadotropins were increased by 82.7%, 48.7%, 41%, 52.6%, respectively. patients also had lower production of acth, higher blood contents of t3, prolactin and testosterone. un ... | 1988 | 2977468 |
| [the role of structural changes in t4 bacteriophage tail proteins]. | conformational changes in bacteriophage tail proteins after heating and ionic strength alteration leading to dissociation of tail sheath have been studied using protein fluorescence, differential scanning microcalorimetry and electron microscopy methods. autonomous structural changes in tube-baseplate proteins have been revealed. they take place under the same conditions as those which release the bonds holding the sheath protein subunits to those of the tube in isolated sheathed tails. the conf ... | 1988 | 2977729 |
| the internal dynamics of gene 32 protein-dna complexes studied by quasi-elastic light scattering. | the hydrodynamic properties of large homodisperse single stranded dnas complexed with the helix destabilizing protein of phage t4, the product of gene 32 (gp32), have been measured. the results suggest a size of the binding site between 8 and 10 nucleotides/gp32 molecule, in reasonable agreement with earlier work on a complex between gp32 and single stranded 145 base dna. from static light scattering experiments it is concluded that the persistence length of these complexes is about 30 nm, disti ... | 1988 | 2978009 |
| evidence for contrasuppression in patients with crohn's disease. | patients with crohn's disease (cd) have elevated numbers of vicia villosa agglutinin (vva) binding cells in the peripheral blood. these cells represent a major subset of activated peripheral t cells. vva binding t lymphocytes express either the t8 or the t4 determinant on their cell surface. in contrast in normal controls only a minor subset of peripheral t cell expresses binding sites for vva. the majority of these cells coexpress t8. vva binding t cells display no helper activity. only in a su ... | 1988 | 2978206 |
| il-2 receptor and fc epsilon r2 gene activation in lymphocyte transformation: possible roles of atl-derived factor. | lymphocyte transformation/activation is accompanied by the induction of a variety of the inducible receptors associated with the activation antigens. the p55 chain of the interleukin-2 receptor (il-2r/p55) recognized by anti-tac monoclonal antibody (mab) is expressed on activated t and b cells as well as natural killer (nk) cells. il-2r/p55(tac) is constitutively expressed on t4(+) t cells transformed by human t-lymphotropic virus i (htlv-i), which is a causative agent for adult t-cell leukemia ... | 1988 | 2978620 |
| construction of an escherichia coli vector containing the major dna adduct of activated benzo[a]pyrene at a defined site. | the mutagenic and carcinogenic substance benzo[a]pyrene reacts with dna following activation to its corresponding 7,8-diol 9,10-epoxide (bpde), and the major dna adduct (bp-n2-gua) is formed when the c(10)-position of bpde reacts with the n2-position of guanine. it is unknown if this adduct is a premutagenic lesion in vivo. herein, the construction and characterization of an m13mp19-based, e. coli vector that contains bp-n2-gua located in the unique psti restriction endonuclease recognition site ... | 1988 | 2979726 |
| cellular immune mechanisms in the pathogenesis of crohn's disease. | crohn's disease is a chronic relapsing inflammatory disease of the intestine of unknown cause. it has been suggested that the disease may result from an abnormality of the immunological functions of the gut. recent advances in the study of the gastrointestinal immune system show that t cells in the intestinal mucosa are more activated, contain a higher proportion of t4 cells having the phenotypic and functional characteristics of helper-inducer cells, have greater capacity for il-2 production, a ... | 1988 | 2979807 |
| neonatal thyroid disease: differential expression in three successive offspring. | neonatal thyroid disease, manifested as either hyperthyroidism or hypothyroidism, has been described in the offspring of women with autoimmune thyroid disorders. we report clinical and laboratory observations in a mother with autoimmune thyroiditis who gave birth to three children with different forms of thyroid dysfunction. the first child was clinically normal, the second child had transient neonatal hyperthyroidism, and the third child had neonatal hypothyroidism. after the birth of the secon ... | 1988 | 2895120 |
| dibutyryl camp induction of type ii 5'deiodinase activity in rat brain astrocytes in culture. | dibutyryl camp treatment of cultured rat astrocytes results in the rapid appearance of t4 to t3 conversion catalyzed by type ii iodothyronine 5'deiodinase, without altering other deiodinating pathways. induction of enzyme activity was time-dependent with a lag period of 60 min, reaching plateau levels after 6-8 hours, and required continued synthesis of mrna and new protein. isoproterenol also induced t4 to t3 converting activity through beta-adrenergic receptor mediated interactions. these data ... | 1988 | 2895644 |
| neonatal t4+ lymphocytes: analysis of the expression of 4b4 and 2h4 antigens. | using monoclonal antibodies anti-2h4 and anti-4b4 we investigated by double immunofluorescence and immunorosette technique the presence of cells displaying the helper-inducer and the inducer of suppression phenotype among t4+ cord blood cells. the analysis of the estimated frequencies of subpopulations of t4+ cells shows that a high percentage of t4+ cord blood cells can coexpress both antigens in comparison to adult. furthermore the percentage of t4+ cells with helper-inducer phenotype (2h4-4b4 ... | 1988 | 2961493 |
| generation of cd8 (t8) cytotoxic cells has a preferential requirement for cd4+2h4- inducer cells. | cd8 (t8) cells are capable of both suppression and cytotoxicity. however, we have found that the activation of cd8 cytotoxic cells has a preferential requirement for a different cd4 (t4) subset from that previously reported for the activation of cd8 suppressor cells. we have recently characterized two monoclonal antibodies which subdivide cd4 cells into inducers of help for antibody production (cd4+ 4b4+) and inducers of cd8 mediated suppression (cd4+2h4+). we now report that cd4+4b4+2h4- cells ... | 1988 | 2962747 |
| cd11 molecule defines two types of suppressor cells within the t8+ population. | it has been shown that t8+ cells are comprised of functionally heterogeneous subpopulations such as suppressor, cytotoxic, and nk cells. in this report, we attempted to delineate the functional heterogeneity of t8 cells defined by anti-cd11 antibody (anti-mol). although allospecific cytotoxic activity was restricted to the t8+mol- subset, suppression of pwm igg synthesis could be elicited in both the t8+mol+ and the t8+mol- subset of cells. however, the mechanism of suppression was different in ... | 1988 | 2962748 |
| induction of helper and suppressor t cells by nonoverlapping determinants on the large protein antigen, beta-galactosidase. | the fine specificity of the t cell repertoire directed against t helper (th)-inducing and t suppressor (ts)-inducing determinants was examined with cyanogen bromide and tryptic peptides of escherichia coli beta-galactosidase (gz), a large tetrameric protein (monomer molecular weight = 116 kda). immunization with cyanogen bromide fragment 2 [cb-2, amino acids (a.a.) 3-92] induced both specific th and ts cells. study of the induction of these functionally opposite t cell subpopulations with trypti ... | 1988 | 2963778 |
| increased helper inducer and decreased suppressor inducer phenotypes in the rheumatoid joint. | cells isolated from the joints of patients with rheumatoid arthritis (ra) exhibit functional immune abnormalities, such as diminished suppressor activity, depressed response to mitogens, and enhanced immunoglobulin production. we sought to characterize the t lymphocyte subsets in the synovial fluid (sf) and peripheral blood (pb) of ra patients in an attempt to clarify the mechanism(s) responsible for these functional immune abnormalities. we used dual-immunofluorescence staining techniques with ... | 1988 | 2964240 |
| regulation of fructose 2,6-bisphosphate levels in cold-acclimated brown adipose tissue of rat. | the effects of cold exposure and t4 administration on fructose 2,6-bisphosphate levels, phosphofructokinase-2 and pyruvate kinase activities were examined in rat brown adipose tissue. cold adaptation (14 days) gave rise to a 2-fold increase in the amount of fructose 2,6-bisphosphate and phosphofructokinase-2 activity, and increased the pyruvate kinase activity 4-fold. if, in addition, the cold-acclimated rats were treated with t4, these parameters were again significantly enhanced. the effect on ... | 1988 | 2964380 |
| nucleotide sequence of the tail sheath gene of bacteriophage t4 and amino acid sequence of its product. | the nucleotide sequence of gene 18 of bacteriophage t4 was determined by the maxam-gilbert method, partially aided by the dideoxy method. to confirm the deduced amino acid sequence of the tail sheath protein (gp18) that is encoded by gene 18, gp18 was extensively digested by trypsin or lysyl endopeptidase and subjected to reverse-phase high-performance liquid chromatography. approximately 40 peptides, which cover 88% of the primary structure, were fractionated, the amino acid compositions were d ... | 1988 | 2964531 |
| treatment of the acquired immunodeficiency syndrome (aids) and aids-related complex with a regimen of 3'-azido-2',3'-dideoxythymidine (azidothymidine or zidovudine) and acyclovir. a pilot study. | on the basis of observation that acyclovir potentiates the in-vitro antiviral activity of 3-azido-2',3'-dideoxythymidine (also known as azidothymidine or zidovudine) against human immunodeficiency virus (hiv), we administered a regimen of azidothymidine and acyclovir to eight patients with the acquired immunodeficiency syndrome (aids) or aids-related complex. an oral regimen of 100 mg of azidothymidine and 800 mg of acyclovir every 4 hours was in general well tolerated, with the principal toxici ... | 1988 | 2964805 |
| mechanism of t cell-derived helper factor production upon stimulation with pokeweed mitogen in humans. | t cell-derived helper factors can cause activated b cells to proliferate and differentiate into immunoglobulin (ig)-secreting cells. in the pokeweed mitogen (pwm)-driven ig production system, the cell-cell interaction necessary for the production of the helper factors was analysed. t cells were pulsed with pwm with or without autologous monocytes. the t cells were then isolated, and incubated for 48 h. supernatants were tested for their ability to promote differentiation of activated b cells. we ... | 1988 | 2964963 |
| modulation of escherichia coli recbcd activity by the bacteriophage lambda gam and p22 abc functions. | plasmids that express the bacteriophage lambda gam gene or the p22 abc2 gene (with and without abc1) at controllable levels were placed in escherichia coli and tested for effects on the activity of recbcd. like gam, abc2 inhibited the atp-dependent exonuclease activity of recbcd, apparently not by binding to dna. however, abc2-mediated inhibition was partial, while gam-mediated inhibition was complete. both abc2 and gam inhibited host system-mediated homologous recombination in a chi-containing ... | 1988 | 2966143 |
| abnormalities in cd4+ t-lymphocyte subsets in inflammatory rheumatic diseases. | the monoclonal antibodies anti-2h4 and anti-4b4 identify the suppressor-inducer (cd4+2h4+) and helper-inducer (cd4+4b4+) subpopulations of cd4 (t4+) lymphocytes, respectively. the cell surface phenotype of peripheral blood lymphocytes and synovial fluid lymphocytes in patients with rheumatoid arthritis and other inflammatory joint diseases was analyzed by use of these and other well-characterized anti-t-cell monoclonal antibodies. in the synovial fluid of patients with rheumatoid arthritis, ther ... | 1988 | 2966579 |
| human gamma interferon induction by staphylococcal protein a: effector cells, kinetics and the effect of prostaglandin, indomethacin, ibuprofen and aspirin. | soluble staphylococcal protein a (spa) induces the synthesis of gamma-interferon (gamma-ifn) by human peripheral blood lymphocytes (pbl). to investigate the kinetics of this gamma-ifn induction and the effector cells involved, we used a highly purified spa preparation, pbl from healthy volunteers, and a cpe-inhibition gamma-ifn assay with sindbis virus in human fibroblasts. the production of spa-induced gamma-ifn (spa-gamma-ifn) peaked 48 h after the addition of spa to cultures of pbl and decrea ... | 1988 | 2966775 |
| t4+ t helper cell function in vivo: differential requirement for induction of antiviral cytotoxic t-cell and antibody responses. | this study documents the differential requirements of t4+ t helper cells in the induction of virus-specific cytotoxic t-lymphocyte (ctl) and antibody responses during acute lymphocytic choriomeningitis virus infection. two monoclonal antibodies (gk1.5 and rl172.4) directed against the l3t4 (t4) molecule were used for depleting t helper cells from mice. depletion of t4+ cells caused a pronounced suppression of antiviral antibody response (20-fold decrease) but had minimal effect on virus-specific ... | 1988 | 2966865 |
| primary hypothyroidism in severe chronic heart failure. | thyroid function was studied in 40 patients with chronic heart failure. thyroid antibodies and microsome antibodies were negative in all cases. serum t4, and t3 concentrations showed significant inverse correlation with cardiothoracic ratio, mean right atrial pressure, pulmonary artery systolic pressure, and peripheral venous pressure. serum t4, t3 concentrations showed significant correlation with pao2, serum albumin, and serum cholinesterase. serum tsh concentrations increased with increasing ... | 1988 | 2966870 |
| hypothyroidism and pituitary contents of immunoactive met-enkephalin and beta-endorphin in male rats of different ages. | a comparison of the effect of ptu-induced hypothyroidism on the contents of immunoactive met-enkephalin and beta-endorphin in the pituitaries from rats aged 2 1/2, 12, and 18 months was made. in all ages, there was significant reduction of ir-met-enkephalin content in the anterior lobe and ir-beta-endorphin content in the neuro-intermediate lobe after ptu treatment. there was a significant age-related decrease in ir-beta-endorphin content in the anterior lobe. rats of all three ages responded to ... | 1988 | 2966963 |
| the clinical neuromuscular pharmacology of mivacurium chloride (bw b1090u). a short-acting nondepolarizing ester neuromuscular blocking drug. | mivacurium chloride (bw b1090u), a bis-benzylisoquinolinium diester compound, was found to undergo hydrolysis in vitro by purified human plasma cholinesterase in a ph-stat titrator at 88% of the rate of succinylcholine at ph 7.4, 37 degrees c and 5 microm substrate concentration. in 72 consenting asa physical status i-ii patients receiving nitrous oxide/oxygen-narcotic-thiopental anesthesia, the neuromuscular blocking effect of mivacurium was assessed following bolus doses from 0.03 to 0.30 mg/k ... | 1988 | 2967039 |
| defective helper function of purified t4 cells and excessive suppressor activity of purified t8 cells in patients with b-cell chronic lymphocytic leukemia. t4 suppressor effector cells are present in certain patients. | we investigated helper and suppressor functions to b-cell responses and t-t cell interactions of purified t4 and t8 cells from 20 untreated patients with b-cell chronic lymphocytic leukemia (cll). appropriate mixtures of purified t4 or t8 cells from patients with cll were cultured with purified b cells or t4 and b cells from normal donors for 7 days with pokeweed mitogen (pwm). igm, iga, and igg produced were determined in the supernatants of these cultures by a heavy chain-specific enzyme-linke ... | 1988 | 2967096 |
| t cell-dependent activation of b cell proliferation and differentiation by immobilized monoclonal antibodies to cd3. | the capacity of mab directed at the cd3 molecular complex (64.1) to induce t cell-dependent b cell proliferation and differentiation was examined. coculture of b cells with mitomycin c-treated t4 cells (t4 mito) stimulated by immobilized 64.1 resulted in marked b cell proliferation and ig-secreting cells (isc) generation in the absence of any additional stimulation. the magnitude of the b cell responses induced by immobilized 64.1-stimulated t4 mito was far greater than that induced by other sti ... | 1988 | 2967324 |
| formation of complexes between long tail fibres and substructural elements of phage t4d. | complexes of substructural elements of bacteriophage t4 (baseplates, baseplate-core complexes) with long tail fibres were obtained for the first time by complementation in vitro. a study of the organization of the complexes was carried out by page, electron microscopy and sedimentation analysis. about 90% of baseplates and baseplate-core complexes were combined with fibres. however, the number of the attached fibres varied from one to six. on the basis of the data obtained, we proposed that the ... | 1988 | 2967347 |
| prognostic value of langerhans cells in the epidermis of hiv patients. | we measured the number of langerhans cells in clinically normal epidermis and the number of t4 lymphocytes in the plasma in 24 patients infected with hiv, and related these to the stage of the disease. a correlation is demonstrated between the numbers of langerhans cells and t4 lymphocytes. numbers of langerhans cells were lower in patients with disease stages iii and iv than in those with disease stage ii. thus langerhans cell numbers could be of prognostic value in this disease. | 1988 | 2967714 |
| role of the 2h4 molecule in the activation of suppressor inducer function. | the monoclonal antibody anti-2h4 recognizes a 220-kda and 200-kda glycoprotein and subdivides t4+ cells into distinct subpopulations: the t4+2h4+ inducer of suppression and the t4+2h4- inducer of help. the t4+2h4+ subset has been shown to play a crucial role in the activation of t8+ suppressor cells. in the present study, we attempted to determine whether the 2h4 molecule itself may be involved in initial triggering of suppressor inducer function. the results show that the addition of anti-2h4 a ... | 1988 | 2967762 |
| the mechanism of homologous dna strand exchange catalyzed by the bacteriophage t4 uvsx and gene 32 proteins. | a strand exchange reaction between a single-stranded dna circle and a homologous linear double-stranded dna molecule is catalyzed by a mixture of two t4 bacteriophage proteins, the uvsx protein (a dna-dependent atpase that resembles the reca protein) and the gene 32 protein (a helix-destabilizing protein). the products are different from those formed in the corresponding reca protein-catalyzed reaction; rather than producing a linear single strand plus a nicked circular double-stranded (form ii) ... | 1988 | 2967823 |
| bacterial infections in human immunodeficiency virus-infected children. | a retrospective review of 71 children infected with human immunodeficiency virus cared for over a 3.5-year period revealed that 44 of 71 (63%) required a bacterial culture and 27 of 71 (37%) had bacteriologically documented infection. there were 125 episodes in 27 patients. pneumonia (24 of 125 (19%)), upper respiratory tract syndromes (23 of 125 (19%)), urinary tract infection (24 of 125 (19%)) and wound infection (12 of 125 (10%)) were the most common syndromes identified. bacteremic infection ... | 1988 | 2967948 |
| thyroxine-induced cardiac hypertrophy: time course of development and inhibition by propranolol. | to investigate the mechanism of thyroid hormone-induced cardiac hypertrophy, we have studied the in vivo changes in cardiac size and total myocardial content of both membrane and cytoskeletal enzymes in the rat after the administration of excess thyroid hormone. in response to 50 micrograms t4/day, there is a significant increase in heart rate and heart work associated with an increase in total heart size and protein content. measurements of the specific activity of na,k-atpase and p-nitrophenol ... | 1988 | 2968237 |
| modulation of atrial natriuretic factor by thyroid hormone: messenger ribonucleic acid and peptide levels in hypothyroid, euthyroid, and hyperthyroid rat atria and ventricles. | the effect of thyroid hormone on atrial natriuretic factor (anf) production was investigated in hypothyroid, euthyroid, and hyperthyroid rats by measuring levels of anf mrna and anf in myocardium. anf mrna was quantitated by dot blot hybridization, and anf by specific ria. relative anf mrna concentrations (anf mrna to 18s rna) were determined for right atria, left atria, and ventricular apices. the total chamber content of anf mrna was estimated (concentration x total chamber rna) and used as a ... | 1988 | 2968239 |
| treatment of locally advanced prostatic carcinoma with lhrh analogues: cytological, dna-cytophotometrical, and clinical results. | from june 1, 1981 to december 31, 1985, 122 patients aged 54 to 83 years, with locally advanced prostatic carcinoma, were treated with buserelin. nineteen of the patients received combined therapy with buserelin and androcur for the first 3 months. to control the response of the primary tumor to therapy, fine-needle aspiration biopsy of the prostate was made in all patients at 3-month intervals. fifty-eight (76.3%) of 76 patients with locally advanced prostatic carcinoma, with or without bone me ... | 1988 | 2968759 |
| calcium channel blocker inhibition of the calmodulin-dependent effects of thyroid hormone and milrinone on rabbit myocardial membrane ca2+-atpase activity. | the ca2+-atpase activity of rabbit myocardial membranes is stimulated in vitro by l-thyroxine and by milrinone, a bipyridine. these effects are concentration dependent and calmodulin requiring. the calcium channel blockers nifedipine and verapamil have been reported to have anti-calmodulin effects in other assay systems. in this study we have examined the effects of nifedipine and verapamil on rabbit myocardial membrane ca2+-atpase activity, in the absence (basal activity) and presence of exogen ... | 1988 | 2968801 |
| monoclonal antibody 4b4 blocks suppression of the immune response to cryptomeria japonica pollen antigen. | the monoclonal antibody 4b4 blocked the t8+ t cell-mediated suppression of ige response to cryptomeria japonica pollen antigen (cpag). the helper t cells for ige response to cpag expressed 4b4 specificity, whereas the suppressor t cells did not. the 4b4 molecule on pbl was a heterodimer and three additional bands were detected on activated cpag-specific t4+ t cells. they were not closely associated with hla molecules. it is thus conceivable that the 4b4 molecule is not only a marker of helper t ... | 1988 | 2968965 |
| [soluble t4 molecules inhibit hiv infection in vitro]. | 1988 | 2969359 | |
| cloning of a complementary deoxyribonucleic acid coding for human thyroxine-binding globulin (tbg): existence of two tbg messenger ribonucleic acid species possessing different 3'-untranslated regions. | an adult human liver cdna library constructed in expression vector, bacteriophage lambda gt11, was screened with polyclonal antibody directed against human t4-binding globulin (tbg). tbg cdna cloned in the present study was 944 nucleotides in length. it contained approximately 70% of the coding region and complete 3'-untranslated region. when the sequence was compared with that of tbg cdna recently cloned by i. l. flint, t. j. bailey, t. a., gustafson, b. e. markham, and e. morkin, the 3'-untran ... | 1988 | 2969453 |
| human t-lymphocyte subpopulations in periapical lesions. | both b-lymphocyte-and t-lymphocyte-mediated immunologic reactions have been implicated in the pathogenesis of human periapical lesions. the purpose of this study was to identify various subpopulations of t-lymphocytes in these lesions. fifteen periapical lesions were obtained during periapical surgery. each specimen was quick-frozen and stored in liquid nitrogen. immunoperoxidase staining for the presence of t-lymphocyte subpopulation, was done with the use of the monoclonal antibody and vecta-s ... | 1988 | 2969495 |
| leukaemic b cells from patients with chronic lymphocytic leukaemia suppress immunoglobulin production by lymphocytes from normal donors. | we observed that highly purified e-rosette-negative largely leukaemic b cells from 9 out of 15 patients with chronic lymphocytic leukaemia (cll) significantly suppressed immunoglobulin production by mixtures of t4 and b cells from normal donors in the presence of pokeweed mitogen (pwm). this suppression by leukaemic b cells was concentration-dependent. addition of equal numbers of b cells from normal donors to the mixtures of normal t4 and b cells increased, or had no effect on the production of ... | 1988 | 2969611 |
| [bronchoalveolar lavage in patients with sarcoidosis--methodologic aspects of the collection, immunologic characterization of lymphocytes and studies of immunoglobulin content]. | the activity of alveolitis in 25 patients with pulmonary sarcoidosis was determined by analysis of t-lymphocytes and their subpopulations collected by broncho-alveolar lavage. the characterization of the lymphocytes was performed with monoclonal antibodies (bl-series). an amount of t-lymphocytes higher than 28% of all cells and a proportion of t4/t8-lymphocytes of 8:1 and over are signs of the activity of the alveolitis and an indication for corticoid therapy. | 1988 | 2969649 |
| monocyte-independent interleukin-2 production and proliferation of human t cells in response to murine hybridomas expressing the okt3 monoclonal antibody: interleukin-1 is not required for t-cell proliferation. | we report a new, monocyte-independent system for the induction of activation and proliferation of human t cells in response to murine hybridomas expressing the okt3 monoclonal antibody (okt3 hybridomas). incubation of nylon-wool-nonadherent (na) lymphocytes or purified t cells with okt3 hybridomas resulted in interleukin-2 (il-2) production, expression of il-2 receptor, modulation of the cd3 antigen, and proliferation. in contrast, murine hybridomas (okt4, okt8, anti-hla-dr, and others) expressi ... | 1988 | 2969776 |
| differentiation of human b lymphocyte subpopulations induced by an alloreactive helper t-cell clone. | we have used cloned alloreactive helper t cells to determine if direct t cell-b cell interaction can induce differentiation of human peripheral blood b cells which do not respond to pokeweed mitogen (pwm). t-cell clone 2f8 was derived from a one-way mixed lymphocyte reaction. 2f8 cells are t3+t4+t8-il-2r+ and proliferate in response to irradiated stimulator cells, but not autologous cells, in the absence of exogenous interleukin-2. 2f8 cells provide allospecific help for polyclonal proliferation ... | 1988 | 2970473 |
| t4 cell receptor distortion in acquired immune deficiency syndrome. | among the possible affects of the hiv virus on t-cells in acquired immune deficiency syndrome is the cell membrane distortion of the t4 cell protein receptor for genetically related macrophage factor (grf). the integrity of this specific t-cell receptor takes on profound significance due to its relationship to soluble antigen. alpha-tocopheral has been shown to assist in the maintenance of cell membrane structure and may have application in a strategy of membrane restoration. adoptive transfer, ... | 1988 | 2970579 |
| propranolol and thyroxine-induced hypertrophic rabbit hearts: effect on heart size and regional o2 supply/consumption variables. | the purpose of this study was to determine the effect of acute and chronic propranolol on heart size and regional o2 supply/consumption variables in thyroxine (t4)-treated rabbit hearts. new zealand white rabbits were given 0.5 mg/kg t4 for 3 or 16 days with and without concomitant 2 mg/kg propranolol. another group was given 16 days of propranolol alone and another 3-day t4 group was given 2 mg/kg propranolol 1 h before the experiment began. another group served as control. myocardial blood flo ... | 1988 | 2970840 |
| phenotypes of peripheral blood t lymphocytes in rheumatoid arthritis and juvenile rheumatoid arthritis. findings in patients with varying disease activity and clinical subgroups. | the percentage of t4 and t8 positive cells in peripheral blood of 78 patients with rheumatoid arthritis (ra) and 26 patients with juvenile rheumatoid arthritis (jra) was determined using a rosette technique. the t4/t8 ratio +/- sem (standard error of the mean) in the ra patients was increased, 2.02 +/- 0.09, as compared with normal blood donors, 1.71 +/- 0.06 (p less than 0.02). the subgroups of ra patients who had a significantly increased t4/t8 ratio were ana positive patients (p less than 0.0 ... | 1988 | 2970912 |
| the expression of the 35- and 67-kda calcimedins is dependent on thyroid hormone. | we have investigated the expression of the 35- and 67-kda calcimedins and calmodulin during fetal and neonatal periods and in adulthood in rat liver, muscle, and brain. the 35- and 67-kda calcimedin expression in liver and muscle increased during the perinatal period and correlated with the thyroid status of the developing rat. in fact, animals treated with thyroxine demonstrated a precocious appearance of the 35- and 67-kda calcimedin in liver and muscle. animals treated with methylthiouracil, ... | 1988 | 2971036 |
| long-term therapy with a depot luteinizing hormone-releasing hormone analogue (zoladex) in patients with advanced prostatic carcinoma. | we treated 191 patients with histologically proved locally advanced stage (t3 or t4) and/or metastatic prostate cancer with a biodegradable depot formulation of a luteinizing hormone-releasing hormone analogue (zoladex). after an initial increase in serum testosterone in week 1 of therapy a continuous decrease of testosterone to castrate levels was obtained. with a monthly injection of the luteinizing hormone-releasing hormone analogue 4 patients (2 per cent) experienced a transient increase in ... | 1988 | 2971119 |
| suppression associated lymphocyte markers in lesions of sarcoidosis. | the presence within the epithelioid granulomas of sarcoidosis of lymphocytes of the "helper" (t4 positive) phenotype suggests that, as in granulomatous diseases of known aetiology, active cell mediated immunity plays a part in the disease. it has, however, been noted that t8 positive lymphocytes are also found on occasion in the granulomas. the presence of cells bearing markers associated with suppressor activity in various lesions of sarcoidosis has been investigated with a range of monoclonal ... | 1988 | 2971276 |
| nucleotide sequence of the tail tube structural gene of bacteriophage t4. | the nucleotide sequence of gene 19 of bacteriophage t4, the structural gene of the tail tube protein, was determined by both the dideoxy and the maxam-gilbert methods. the predicted mr of tube protein gene product 19 is 18,842. the n-terminal amino acid of the tube protein was determined by edman degradation, and the c-terminal sequence was confirmed by isolation of the c-terminal tryptic peptide. in the noncoding region between genes 18 and 19, there are two late-t4-promoter consensus sequences ... | 1988 | 2963141 |
| expression of t-cell antigens on reed-sternberg cells in a subset of patients with nodular sclerosing and mixed cellularity hodgkin's disease. | expression of t-cell antigens by reed-sternberg (rs) cells has not been detected in most studies of hodgkin's disease (hd). the authors employed an improved method of fixation (paraformaldehyde-lysine-periodate), which sharply defined cell borders and revealed t-cell antigens on rs cells in 8 of 30 (27%) cases of hd. antigen-specific staining was confirmed by immunoelectron microscopy. rs cells expressed t11 (8/8 cases), leu-3 or t4 (4/8 cases), leu-4 or t3 (3/8 cases), but not other t-cell spec ... | 1988 | 2963547 |
| [the role of postoperative radiotherapy in the treatment of hypernephroid carcinoma]. | the following results can be derived from the clinical and radiobiological literature as well as from the authors's own experiences with 91 patients analyzed retrospectively and evaluated with respect to their risk factors. 1. a postoperative irradiation is very probably sensible in patients with tumor perforation through the renal capsule (t3/t4) and with venous or lymph node manifestations. 2. the operation method is essential, above all the transabdominal technique and the quality of lymph di ... | 1988 | 3041639 |
| relationship between the diagnosis, preoperative evaluation, and prognosis after orthotopic liver transplantation. | the purpose of this study was to identify which of the biochemical, immunological, or functional parameters derived before surgery as part of a systemic evaluation were helpful in predicting the frequency of rejection episodes, the chance of survival, and the cause risk of death (should death occur) of patients after orthotopic liver transplantation (oltx). ninety-eight adult patients who had an extensive preoperative protocol evaluation were studied before oltx. the biochemical parameters asses ... | 1988 | 3041926 |
| levels of insulin, corticosterone, t3, t4 and insulin sensitivity in fat and lean chickens. | the late generations of fat (fl) and lean (ll) chickens were compared. in the fasted state, plasma glucose was lower in fl chickens, whereas insulin, t3, t4 and corticosterone were unchanged. in the fed state, plasma insulin and t4 were increased in fl chickens whereas glucose, t3 and corticosterone were unchanged. during ad libitum refeeding both plasma glucose and insulin, and to a lesser degree t3, were lower and t4 higher in fl chickens whereas corticosterone remained unchanged between lines ... | 1988 | 3042492 |
| resting metabolic rate and postprandial thermogenesis in vegetarians and nonvegetarians. | resting metabolic rate (rmr), thermic effect of a meal (tem), and associated hormonal changes were studied in vegetarians and nonvegetarians. rmr was established by indirect calorimetry in 12 male vegetarians (veg) and 11 nonvegetarians (nveg) of similar body fat and aerobic fitness. subjects ingested a liquid meal and tem was measured for 180 min postprandially. plasma concentrations of glucose, insulin and thyroid hormones (t3 and t4) were determined before and after meal ingestion. absolute r ... | 1988 | 3044062 |
| effect of thyroid hormones on oxidative and nonoxidative glucose metabolism in humans. | the glucoregulatory function of thyroid hormones was investigated in six healthy subjects before and after 14 day 3,5,3',5'-tetraiodothyronine (t4) treatment (300 micrograms/day) using a sequential clamp protocol for 5 h at euglycemia (0-2 h) and hyperglycemia (165 mg/dl, 2-5 h) and different insulin infusion rates (1.0 for 0-3.5 h and 6.5 mu.kg-1.min-1, for 3.5-5 h). t4 treatment increased basal energy expenditure (+8%), glucose disposal (+31%), and oxidation (+87%) but decreased nonoxidative g ... | 1988 | 3044137 |
| bone changes in alcoholic liver cirrhosis. a histomorphometrical analysis of 52 cases. | bone biopsies of 52 histologically confirmed alcoholic cirrhotic patients and 15 age- and sex-matched controls have been histomorphometrically analyzed determining trabecular bone volume (tbv), mineralized bone volume (mbv), and osteoid volume (ov). we also determined serum pth, 25-oh-d3, calcitonin, fsh, lh, estradiol, testosterone, t3 and t4, urine cortisol, routine liver function tests, serum and urinary calcium, phosphorus, and magnesium. we found a high prevalence of osteoporosis: tbv was s ... | 1988 | 3044713 |
| molecular analysis of plasmid dna repair within ultraviolet-irradiated escherichia coli. i. t4 endonuclease v-initiated excision repair. | the process by which dna-interactive proteins locate specific sequences or target sites on cellular dna within escherichia coli is a poorly understood phenomenon. in this study, we present the first direct in vivo analysis of the interaction of a dna repair enzyme, t4 endonuclease v, and its substrate, pyrimidine dimer-containing plasmid dna, within uv-irradiated e. coli. a pyrimidine dimer represents a small target site within large domains of dna. there are two possible paradigms by which endo ... | 1988 | 3045127 |
| anal submucosal injection: a new route for drug administration in pelvic malignancies. ii. methotrexate anal injection in the treatment of advanced bladder cancer. preliminary study. | the clinical efficacy of submucosal anal injections of methotrexate in advanced bladder cancer is investigated. an experimental study on 20 mice has shown that methotrexate injected into the anal submucosa has no clinicopathological effect on the anorectum. the clinical study comprised 18 patients with advanced bladder cancer (13 with stage t3 and 5 with stage t4 disease) as a test group in whom methotrexate was injected into the anal submucosa and 8 (6 with stage t3 and 2 with stage t4 cancer) ... | 1988 | 3045340 |
| yeast gene rad52 can substitute for phage t4 gene 46 or 47 in carrying out recombination and dna repair. | the rad52 gene of saccharomyces cerevisiae and genes 46 and 47 of bacteriophage t4 are essential for most recombination and recombinational repair in their respective organisms. the rad52 gene was introduced into expression vectors that were used to transform escherichia coli. the expression of rad52 was then induced, and the ability of rad52 to complement phage mutants defective in gene 46 or 47 was determined with respect to the three criteria of phage growth, recombination, and recombinationa ... | 1988 | 3045825 |
| effects of thyroxine-driven precocious metamorphosis on maturation of adult-type allograft rejection responses in early thyroidectomized frogs. | in contrast to the adult pattern of allograft reactivity, larval south african clawed frogs (xenopus) either become tolerant of adult major histocompatibility complex (mhc) disparate skin grafts or reject them slowly. larvae fail to reject grafts that are incompatible at minor histocompatibility (h) loci (mhc-identical); rather, they become immunologically tolerant. we report here that early thyroidectomized (thyroidx) larvae, forced to metamorphose precociously on a regimen of thyroxine (t4) tr ... | 1988 | 3046979 |
| [langerhans cell: cd1 antigens and the birbeck granule]. | langerhans cells are characterized by two types of markers: an ultrastructural marker, the birbeck granule and different membrane markers: hla-d antigens, t4 antigen, and some of the cd1 antigens. these antigens which are specific for the epidermal langerhans cells, are not expressed by the other epidermal cells. three cd1 antigens are biochemically defined on human thymocytes, they display a glycoprotein chain non covalently attached to beta-2-microglobulin. only two of these glycoproteins: the ... | 1988 | 3047647 |
| lymphocyte subpopulations during acute and convalescence phases of malaria. | lymphocytes of normal healthy persons were separated from blood by ficoll-hypaque gradient centrifugation and iron-magnet application. peripheral blood lymphocytes (pbl) were stained by various dye-labeled monoclonal antibodies. cells positive for specific surface markers were enumerated by a fluorescence activated cell sorter (facs) and fluorescence microscope (fm). the results revealed that the percentages of cells positive with one monoclonal antibody counted by these two techniques were simi ... | 1988 | 3048287 |
| sequential trial of initial chemotherapy for advanced cancer of the head and neck. ddp versus ddp + 5-fluorouracil. | the study, which compares ddp to ddp + fu, was planned to detect an increase by 60% in efficacy and by 5% in toxicity (2a = b = 5%) for ddp + fu. in a previous trial ddp produced 15% of responders and 5% of high-level toxic manifestations. the eligible patients with an advanced head and neck cancer were paired off successively on the basis of the tumour site and the uicc stage. ddp (100 mg/m2; day 1) was administered with hyperhydration, alone in the first protocol and followed by a 5-day contin ... | 1988 | 3048643 |