Publications

endocrine effects of combined somatostatin analog and bromocriptine therapy in women with advanced breast cancer.	in this pilot clinical trial conducted in 10 postmenopausal women with advanced breast cancer, we evaluated the endocrine effects and toxicity of combined somatostatin analog and dopaminergic therapy in the attempt to suppress both growth hormone (gh) and prolactin (prl) secretion. the patients' mean age was 63 years (range: 54-77) and the average number of previous treatments was 4.8 +/- 2 (sd). all patients were treated with the somatostatin analog sms 201-995 (100-200 micrograms s.c. in a.m. ...	1989	2575406
plasma glutamate--a prognostic marker of cancer and of other immunodeficiency syndromes?	elevated plasma levels of glutamate (glu) have been reported to occur in patients with malignancies and other immunodeficiency syndromes (ids). to evaluate, whether glu is useful as prognostic indicator, the plasma concentrations were determined in patients with colorectal carcinoma (crc), with breast cancer (brc), and with hiv-infection (hiv). the results were correlated with the disease-stages, and compared with data obtained from patients with benign diseases of the same organ, as well as fro ...	1989	2575787
[sufentanil: the effect on cardiocirculatory parameters and intubation conditions on administration of pancuronium or vecuronium].	a lack of uniform methodology used in the assessment of moderate doses of sufentanil in combination with non-depolarizing neuromuscular blocking drugs formed the basis of the current study which compared under randomized conditions the effects of sufentanil-pancuronium versus sufentanil-vecuronium on hemodynamics, intubating conditions and chest wall rigidity during induction of anesthesia. material and methods. one hundred and twenty asa physical status i and ii patients aged between 20 and 40 ...	1989	2575875
effects of beta-adrenergic blockers with different ancillary properties on lipid peroxidation in hyperthyroid rat cardiac muscle.	to determine whether beta-blockade protects rat heart against thyroxine (t4)-induced accelelation of lipid peroxidation, in vivo effects of 3 beta-blockers with different ancillary properties on the mitochondrial oxidative enzyme, antioxidant enzymes and lipid peroxide were investigated. the rats were rendered hyperthyroid by adding t4 to their drinking water for 3 weeks and were treated simultaneously with either carteolol (a blocker with partial agonist activity; 30 mg/kg/day), atenolol (50 mg ...	1989	2575990
does t4 lymphocyte depletion predispose to hiv infection?		1989	2576020
[novel immunotherapeutic models for neoplastic diseases of the urogenital tract with special attention to bladder carcinoma].	in this review article some novel immunotherapeutic models as well as some new concepts concerning the unspecific and specific t-cell stimulation are discussed briefly. some of these immunotherapeutic models are restricted to bladder cancer; the others have a generalized meaning. one model, restricted to bladder cancer, is based on the enzymatic++ pretreatment of effector-cells (macrophages, nk-cells), followed by their instillation in the patient's bladder. the other bladder tumor restricted mo ...	1989	2576184
complement-dependent cytotoxicity of antibodies reactive with hiv-induced cell surface antigens in hiv-carrying haemophiliacs.	sera obtained from hiv-infected as well as uninfected haemophiliacs and from healthy subjects were investigated for the presence of lymphocytotoxic antibodies. using the 51cr-release test, hiv-infected haemophiliacs were found to produce serum antibodies exerting complement-dependent cytotoxic effect on hiv-infected t4 cells. the antibodies were reactive mainly when hiv-infected target cells were stimulated with concanavalin-a. results of complement-dependent antibody cytotoxicity and indirect m ...	1989	2576594
improved genomic blot hybridization.	for restriction fragment length polymorphism (rflp) and other molecular biology experiments, well prepared (undamaged) genomic dna is needed, which is then digested with restriction enzymes, transferred to nitrocellulose paper and hybridized with radioactive or nonradioactive probe. dna damage occurs in fields such as clinical genetics and forensic medicine in the form, for example, of single stranded breaks. if these dnas are treated with t4 dna ligase prior to restriction endonuclease digestio ...	1989	2576631
role of exonuclease iii and endonuclease iv in repair of pyrimidine dimers initiated by bacteriophage t4 pyrimidine dimer-dna glycosylase.	the role of exonuclease iii and endonuclease iv in the repair of pyrimidine dimers in bacteriophage t4-infected escherichia coli was examined. uv-irradiated t4 showed reduced survival when plated on an xth nfo double mutant but showed wild-type survival on either single mutant. t4 denv phage were equally sensitive when plated on wild-type e. coli or an xth nfo double mutant, suggesting that these endonucleases function in the same repair pathway as t4 pyrimidine dimer-dna glycosylase. a uvra mut ...	1989	2468648
evidence against the importance in the disease process of antibodies to bovine thyroid-stimulating hormone found in some patients with graves' disease.	the antibody to tsh (tsh-ab) found in some patients with graves' disease may be either an antiidiotype (anti-id-ab) to the tsh receptor antibody (trab) or the antigen (idiotype) for which the anti-id-ab is in fact trab. four groups have found antibodies to bovine tsh (btsh-ab) in graves' disease patients in a tsh binding-inhibiting immunoglobulin (tbii) rra that uses [125i]btsh. in this assay serum samples containing btsh-ab give highly negative tbii values. the purpose of this study was to look ...	1989	2909548
detection by 32p-postlabeling of thymidine glycol in gamma-irradiated dna.	the 32p-postlabeling method has been adapted for the analysis of thymidine-cis-glycol-3'-phosphate (cis-dtgp,cis-5,6-dihydroxy-5,6-dihydrothymidine-3'-phosphat e). cis-dtgp was isolated and purified from normal nucleotides by phenylboronate affinity chromatography and phosphorylated by t4 polynucleotide kinase in presence of 1 mm becl2 at ph 7.5. these modifications of the postlabeling method resulted in a 5'-phosphorylation of dtgp with a labeling efficiency of up to 20% whereas the natural nuc ...	1989	2910530
thyroxine transport and distribution in nagase analbuminemic rats.	the postulate that thyroxine (t4) in plasma enters tissues by protein-mediated transport or enhanced dissociation from plasma-binding proteins leads to the conclusion that almost all t4 uptake by tissues in the rat occurs via the pool of albumin-bound t4 (pardridge, w. m., b. n. premachandra, and g. fierer. 1985. am. j. physiol. 248:g545-g550). to directly test this postulate, and to test more generally whether albumin might play a special role in t4 transport in the rat, we performed in vivo ki ...	1989	2910905
comparison of maternal to fetal transfer of 3,5,3'-triiodothyronine versus thyroxine in rats, as assessed from 3,5,3'-triiodothyronine levels in fetal tissues.	thyroxine (t4) is transferred from the mother to the hypothyroid rat fetus late in gestation, mitigating t4 and t3 deficiency in fetal tissues, the brain included. we have now compared the effects of maternal infusion with t3. normal and thyroidectomized rats were started on methimazole (mmi) on the 14th day of gestation, given alone, or together with a constant infusion of 0.45 micrograms (0.69 nmol) t3 or of 1.8 microgram (2.3 nmol) t4/100 g per day. maternal and fetal samples were obtained at ...	1989	2911938
tumor necrosis factor enhances replication of human immunodeficiency virus (hiv) in vitro.	the effect of tumor necrosis factor (tnf) on the replication of human immunodeficiency virus type 1 (hiv-1) was investigated in several t4 lymphocyte cell lines. tnf markedly enhanced the cytopathogenicity of hiv-1, virion-associated reverse transcriptase (rt) activity in the cell culture supernatant, and viral antigen expression in molt-4 cells as early as 3 days after hiv-1 infection. a slight increase in rt activity was also observed in the supernatant of h9 cell cultures exposed to tnf. howe ...	1989	2912452
triiodothyronine, thyroxine, and tsh response to dexamethasone in depressed patients and normal controls.	in view of recent investigations concerning alterations of thyroid function in depression, the pre- and postdexamethasone levels of t3, t4, and tsh of 14 patients during depression and after recovery were studied, in addition to those of 27 healthy controls. a reduction of t3 and tsh levels was shown to be dependent on the depressive state, with a tendency to lower t4 levels after recovery. dexamethasone had a pronounced suppressive effect on tsh levels in healthy controls and in patients after ...	1989	2912508
vasoactive intestinal peptide in the anterior pituitary is increased in hypothyroidism.	vasoactive intestinal peptide (vip) and prl have been reported to be colocalized in rat lactotropes. to determine whether induced hypothyroidism, known to reduce pituitary prl concentration, also reduces pituitary concentration of vip, rats were treated with antithyroid drugs for 3 weeks. pituitary prl concentration in male rats (micrograms/mg protein) was markedly reduced by this treatment (9.4 +/- 1.0 vs. 2.3 +/- 0.4 when extracted at ph 1.1, 17.9 +/- 3.0 vs. 3.4 + 0.4 when extracted at ph 7.4 ...	1989	2912685
carbohydrate reactivation of thyroxine 5'-deiodinase (type ii) in cultured mouse neuroblastoma cells is dependent upon new protein synthesis.	the t3 concentration in brain predominantly reflects local production from t4 rather than t3 uptake from the circulating pool. we recently demonstrated that rat brain t3 content is increased by glucose feeding compared to chow feeding. one possible mechanism for this effect is an increase in brain t4 5'-deiodinase (5'-d) activity. our recent preliminary studies of neuroblastoma (nb) cells demonstrate that renewal of rpmi-1640 medium stimulates t4 5'-d type ii (nb t4 5'-d ii) activity in these ce ...	1989	2912691
increased plasma 3,5,3'-triiodothyronine sulfate in rats with inhibited type i iodothyronine deiodinase activity, as measured by radioimmunoassay.	in contrast to the glucuronide conjugate, t3 sulfate (t3s) undergoes rapid deiodinative degradation in the liver and accumulates in rats and rat hepatocyte cultures if type i iodothyronine deiodinase activity is inhibited. we here report the ria of plasma t3s in rats treated with the antithyroid drugs propylthiouracil (ptu) or methimazole (mmi), of which only ptu inhibits type i deiodinase. male wistar rats were treated acutely by ip injection with 1 mg ptu or mmi/100 g bw and subsequently for 4 ...	1989	2912698
effect of thyroid hormone and growth hormone on recovery from hypothyroidism of epiphyseal growth plate cartilage and its adjacent bone.	hypothyroidism was induced in young female sprague-dawley rats by the addition of methimazole (0.67 mg/ml) to drinking water for a period of 7 weeks (7-14 weeks of age). the responses of the articular cartilage, epiphyseal growth plate cartilage, epiphyseal trabecular bone, and metaphyseal trabecular bone in the proximal tibia were assessed by structural parameters. in addition, replacement therapies were introduced for the last 2 weeks of the experimental period. these included 0.7 u/kg bw huma ...	1989	2912707
uptake of 3,5,3'-triiodothyronine by cultured rat hepatoma cells is inhibitable by nonbile acid cholephils, diphenylhydantoin, and nonsteroidal antiinflammatory drugs.	cellular uptake of t3 was examined using rat h4 hepatoma cells. uptake of [125i]t3 (10(-11) m) from serum-free medium was measured as the cell-associated counts retained by washed cells (2 x 10(6) per well). displaceable uptake was 84% of total uptake at 2 min (2.9% of total counts). t4, tetraiodothyroacetic acid, triiodothyroacetic acid, rt3, and d-t3 were 2-5% as effective as t3 in displacing uptake. nonequilibrium kinetics indicated a half-maximal uptake at 680 nm t3 with approximately 7 mill ...	1989	2912709
effects of photoperiod, temperature and testosterone-treatment on plasma t3 and t4 levels in the djungarian hamster, phodopus sungorus.	the effects of photoperiod, temperature and testosterone treatment on plasma t3 and t4 levels were investigated in the djungarian hamster. plasma t3 level was affected by temperature (25 degrees c less than 7 degrees c) but not by photoperiod. plasma t4 level was affected by photoperiod (short day less than long day) at 25 degrees c. administration of testosterone increased plasma t4 level under short photoperiod at 25 degrees c. thus, higher plasma t4 level under long photoperiod at 25 degrees ...	1989	2912765
postoperative irradiation for squamous cell carcinoma of the head and neck: an analysis of treatment results and complications.	one hundred thirty-four patients with advanced head and neck cancer were treated with radical surgery and postoperative radiation therapy between october 1964 and october 1984. all patients had greater than or equal to 2 years and 84% had greater than or equal to 5 years of follow-up. all patients included in the study were scheduled to receive continuous-course irradiation following a major cancer operation for previously untreated squamous cell carcinoma of the oral cavity, oropharynx, hypopha ...	1989	2912947
altered responsiveness to sympathetic nerve stimulation and agonists of isolated left atria of diabetic rats: no evidence for involvement of hypothyroidism.	to clarify the role of hypothyroidism in diabetes-induced sympathetic neuropathy, we examined the responsiveness to sympathetic nerve stimulation and to agonists of the left atria of streptozotocin-induced diabetic rats, and compared it with those in hypothyroid, thyroxine(t4)-treated diabetic or t4-treated hypothyroid rats. positive inotropic response of isolated left atria to transmural nerve stimulation (tns) was examined in the presence of atropine. in diabetic rats, plasma triiodothyronine ...	1989	2913281
nuclear deoxyribonucleic acid ploidy in squamous cell bladder cancer.	flow cytometric analysis of nuclear deoxyribonucleic acid content was performed on 76 primary squamous cell bladder carcinomas treated between january 1970 and december 1975. patients were followed for a median of 10.1 years. nuclei were extracted from paraffin-embedded archival material and isolated nuclei were stained with propidium iodide. of the 76 tumors 73 were evaluable by flow cytometry providing high quality deoxyribonucleic acid histograms: 27 (37 per cent) showed a deoxyribonucleic ac ...	1989	2913348
paranasal sinus malignancy: a comprehensive update.	a retrospective analysis of 60 cases of paranasal sinus cancer in patients admitted between 1970 and 1985 was undertaken. forty-six tumors originated in the maxillary sinus, and 14 originated in the ethmoid sinuses. computed tomography, magnetic resonance imaging, and endoscopic sinus examination aided in early diagnosis of sinus cancer. computed tomography aided in staging tumors; caldwell-luc alone was inadequate for staging tumors invading deeper sites such as the orbits or pterygoid muscle. ...	1989	2913425
a proton and fluorine-19 nuclear magnetic resonance and fluorescence study of the binding of some natural and synthetic thyromimetics to prealbumin (transthyretin).	interactions between prealbumin and several thyromimetic compounds have been examined by proton magnetic resonance spectroscopy. one equivalent of thyroxine (t4) or reverse triiodothyronine (rt3) selectively broadens a number of protein signals, while addition of a second equivalent induces much less widespread changes. one equivalent of triiodothyronine (t3), however, produces much less dramatic changes, and effects comparable with t4 and rt3 are only apparent when a second equivalent binds. th ...	1989	2914891
affinity of single- or double-stranded oligodeoxyribonucleotides containing a thymine photodimer for t4 endonuclease v.	a gene for t4 endonuclease v was constructed by joining chemically synthesized oligodeoxyribonucleotides and expressed efficiently in escherichia coli under the control of the e. coli tryptophan promoter. overproduced t4 endonuclease v, which can cleave thymine photodimers as well as the corresponding phosphodiester linkage of dna, was used to investigate the precise mode of the reaction with single- or double-stranded synthetic dna fragments containing a thymine photodimer. the substrates, thre ...	1989	2914925
t4, t3 and rt3 levels in serum and cerebrospinal fluid of patients with amyotrophic lateral sclerosis.	thyronine (t4), triiodothyronine (t3), and reverse-triiodothyronine (rt3) levels were evaluated in cerebrospinal fluid (csf) and in serum of 12 patients with definite amyotrophic lateral sclerosis (als) by specific radioimmunoassays. circulating microsomal and thyroglobulin antibodies were also evaluated. in all patients serum levels of t4, t3 and rt3 were within normal limits. in csf, the rt3 levels were significantly elevated to 0.118 micrograms/l (mean), the t4 levels were not significantly e ...	1989	2915230
intraarterial cisplatin and concurrent radiation for locally advanced bladder cancer.	between 1983 and 1987 25 patients with invasive bladder cancer (16 stage tumor 3 (t3) and nine stage t4) were treated with intraarterial cisplatin and concurrent radical radiation (20/25) or intraarterial cisplatin, concurrent preoperative radiation, and cystectomy (5/25). one patient died from treatment-related toxicity. other toxicities have been what one would expect from the individual treatment modalities except for a sensory sacral root neuropathy in 11 of 24 (46%) patients. twenty-three o ...	1989	2915239
[the role of radiotherapy in the treatment of supraglottic laryngeal tumors. case reports].	radiotherapy obtains better results when employed alone, in initial forms (t1-t2), and after surgery, when loco-regional relapse incidence reduces. personal results in the treatment of laryngeal cancer are reported in 281 patients. one-hundred-forty-seven patients treated with exclusive radiotherapy had 5-year global and ned survival of 32.7% and 26.6%; 5-year ned survival in particular was 78.7% for t1-t2 and 22.9% for t3-t4. subsequently 52.8% of patients relapsed. one-hundred-thirty-four pati ...	1989	2915813
control of enzyme activity by an engineered disulfide bond.	a novel approach to the control of enzyme catalysis is presented in which a disulfide bond engineered into the active-site cleft of bacteriophage t4 lysozyme is capable of switching the activity on and off. two cysteines (thr21----cys and thr142----cys) were introduced by oligonucleotide-directed mutagenesis into the active-site cleft. these cysteines spontaneously formed a disulfide bond under oxidative conditions in vitro, and the catalytic activity of the oxidized (cross-linked) t4 lysozyme w ...	1989	2916125
thyroid function in hyperemesis gravidarum.	plasma total t4 (tt4), t3 (tt3), free t4 (ft4), free t3 (ft3), thyroxine binding globulin, hcg, and erythrocyte zinc content were measured in 43 women with uncomplicated pregnancy and in 71 patients admitted with hyperemesis gravidarum. plasma concentration of thyroid hormones in hyperemesis subjects showed wide variability and 32% of subjects had high tt4 (higher than mean +2 sd of normal pregnant subjects), 33% had high ft4, 20% had high tt3, and 20% had high ft3. red cell zinc content, a tiss ...	1989	2916376
effects of chronic peritoneal dialysis on thyroid function tests.	peritoneal dialysis is associated with large losses of protein. in order to quantify thyroid hormone excretion in the dialysate and to examine the possibility that peritoneal dialysis may result in clinical hypothyroidism, nine endstage renal disease (esrd) patients undergoing either continuous ambulatory peritoneal dialysis (capd) or chronic intermittent peritoneal dialysis (ipd) were studied. total protein excretion in the peritoneal fluid was 21.5 +/- 2.1 g/24 h and did not vary with the mode ...	1989	2916576
laser surgery for vocal cord carcinoma involving the anterior commissure.	endoscopic laser surgery is an established means of treatment for benign laryngeal lesions. laser surgery for early (stages i and ii) squamous cell carcinoma is still being tested. treatment of glottic tumors extending to the anterior commissure is in itself controversial. approximately 20% of all glottic tumors involve the anterior commissure, with only 1% of these lesions being purely anterior commissure tumors. the anatomy of the anterior commissure is such that an apparent t1 lesion may actu ...	1989	2916820
partial laryngectomy and radiotherapy for supraglottic cancer: a conservative approach.	this is a retrospective study of 33 patients with supraglottic cancer treated with partial laryngectomy and moderate-dose radiotherapy at the university of virginia from 1967 through 1986. all patients had a 2-year minimum follow-up. ten patients received preoperative radiotherapy, and 23, postoperative radiotherapy. the 2-year adjusted survival rate was impressive at 97%. local control at 2 years was 96%, including one patient who was salvaged with total laryngectomy. control of the neck was 90 ...	1989	2916823
cardiac sympathetic afferent cell bodies are located in the peripheral nervous system of the cat.	studies of the stellate ganglion and middle cervical ganglion indicate that sympathetic efferent nerve activity can be modified by peripheral excitatory inputs and that these neural connections may function as pathways for a peripheral reflex at the level of the thoracic sympathetic ganglia. this excitatory synaptic input could have a soma in either the central or the peripheral nervous system. a study was designed to determine whether chronic decentralization (3 weeks) of the stellate ganglion ...	1989	2917380
increases in thyroid gland blood flow after hemithyroidectomy in the rat.	after subtotal thyroidectomy, the thyroid gland remnant undergoes compensatory alterations in function and morphology. under the trophic stimulation of elevated plasma tsh concentrations, the thyroid remnant responds with an increase in hormone synthesis and secretion and, in addition, increases in mass. we have examined the alterations in thyroid blood flow which accompany increased secretion and growth after hemithyroidectomy (htx) in male sprague-dawley rats (200-220 g). at various times afte ...	1989	2917508
aging alters the activity of 5'-deiodinase in the adenohypophysis, thyroid gland, and liver of the male rat.	aging is characterized by a decreased secretion of thyroid hormones in rats associated with unchanged plasma tsh suggestive of impairments in the hypothalamo-pituitary-thyroid axis. since it is known that pituitary t3 is more determinant on tsh secretion than plasma t3, we measured in young (4 months) and old (26 months) male rats the concentration of t3 in the anterior pituitary gland and found that it was similar in young and old animals despite the low circulating levels of thyroid hormones. ...	1989	2917513
the hormonogenic sites of turtle thyroglobulin and their homology with those of mammals.	thyroglobulin (tg) from turtles previously injected with 125i was reduced, alkylated, and digested with trypsin. we purified the resultant peptides on hplc columns, determined their amino acid sequences and the locations of [125i]t4 and [125i]t3 residues, and compared them with established sequences from humans, cows, rabbits, rats, and guinea pigs. we found five major t4 peptides, three of which were homologous with the major hormonogenic sites a, b, and d of mammalian tg. site a, the highly co ...	1989	2917515
hypophysectomy of the fetal lamb leads to a fall in the plasma concentration of insulin-like growth factor i (igf-i), but not igf-ii.	the role of the pituitary gland in the regulation of the plasma concentrations of insulin-like growth factors (igfs) in the late gestation sheep fetus has been examined. singleton sheep fetuses were either hypophysectomized or sham-operated between days 110-120 of gestation. blood samples were then collected via carotid cannulae at least three times weekly for the remainder of gestation. in some hypophysectomized fetuses t4 was administered (100 g/day) to overcome the hypothyroidism caused by hy ...	1989	2917521
purification, characterization and revised amino acid sequence of a second thioredoxin from corynebacterium nephridii.	a second thioredoxin, distinct from the one reported by meng and hogenkamp in 1981 (j. biol. chem. 256, 9174-9182), has been purified to homogeneity from an escherichia coli strain containing a plasmid encoding a corynebacterium nephridii thioredoxin. thioredoxin genes from c. nephridii were cloned into the plasmid puc13 and transformants were identified by complementation of a thioredoxin negative (trxa-) e. coli strain. the abilities of the transformants to support the growth of several phages ...	1989	2917572
[results of treatment of 584 laryngeal cancers at the ear-nose-throat clinic of marburg university].	the results in the management of 460 vocal cord carcinomas and 124 supraglottic carcinomas are reported. of the vocal cord carcinomas, 63.3% were diagnosed in the early tis and t1 stage. seventy-six tumors were resected endoscopically, 128 by laryngofissure and chordectomy. not one of these patients has lost his life, larynx or voice. in bilateral tumors of the t1b category, 2 patients developed local recurrences and lost their larynx. sixty-two carcinomas of the tis, t1a and t1b categories were ...	1989	2917875
reversible alteration of red cell lithium-sodium countertransport in patients with thyroid disease.	thyroid hormones may alter red blood cell (rbc) sodium content and transport. the functional importance of lithium-sodium (li-na) countertransport in regulating sodium (na) transport in vascular smooth muscle and kidney by na-h countertransport and the potential effect of thyroid hormone on these processes led us to study li-na countertransport and other sodium transporters in rbcs of patients with thyroid dysfunction. patients with untreated hypothyroidism (10) and hyperthyroidism (10) were stu ...	1989	2918049
neurohypophyseal function in postpartum hypopituitarism: impaired plasma vasopressin response to osmotic stimuli.	we studied neurohypophyseal function in 12 women with postpartum hypopituitarism (sheehan's syndrome) by measuring plasma arginine vasopressin concentrations during 5% hypertonic saline infusions. all patients had a history of obstetric shock or massive bleeding, and were receiving cortisol and/or l-t4 replacement therapy. none had any symptoms of diabetes insipidus. the mean basal plasma vasopressin level in the patients [0.6 +/- 0.1 (+/- se) pmol/l] was significantly lower (p less than 0.01) t ...	1989	2918058
extended operation for lung cancer invading the aortic arch and superior vena cava.	extended operation for lung cancer and mediastinal carcinoma involving the aortic arch or superior vena cava was performed in six patients. in three patients with lung cancer invading the right side of the mediastinum, sleeve pneumonectomy (two patients) or sleeve lobectomy was followed by resection and reconstruction of the superior vena cava with ringed polytetrafluoroethylene grafts. one patient with squamous cell carcinoma and t4 n1 m0 disease was alive and free of disease more than 34 month ...	1989	2918738
excision repair characteristics of denv-transformed xeroderma pigmentosum cells.	introduction of the denv gene of phage t4, encoding the pyrimidine dimer-specific endonuclease v, into xeroderma pigmentosum cells xp12ro(m1) was reported to result in partial restoration of colony-forming ability and excision repair synthesis. we have further characterized 3 denv-transformed xp clones in terms of rates of excision of pyrimidine dimers and size of the resulting resynthesized regions following exposure to 100 j/m2 from an fs-40 sunlamp. in the denv-transformed xp cells we observe ...	1989	2918865
microinjection of t4 endonuclease v produced by a synthetic denv gene stimulates unscheduled dna synthesis in both xeroderma pigmentosum and normal cells.	a structural gene for t4 endonuclease v was constructed by ligating synthetic oligonucleotides. the endonuclease v was overproduced in e. coli under control of the e. coli tryptophan promoter and purified to apparent homogeneity. the product had comparable dna glycosylase and apurinic/apyrimidinic (ap) endonuclease activities to the natural enzyme in vitro. when this endonuclease v was microinjected into the cytoplasm of xeroderma pigmentosum (xp) cells of complementation group a, b, c, d, f, g ...	1989	2918866
dysfunction of the monocyte-macrophage system in the idiopathic minimal change nephrotic syndrome.	we studied the function of phagocytes and the distribution of lymphocyte subpopulations in 23 patients with idiopathic minimal change nephrotic syndrome. all the patients were in relapse at the time of the study. the latter was performed before specific therapy was started. our control group consisted of 26 normal children who were studied while undergoing routine analysis prior to plastic surgery. polymorphonuclear leukocytes from the patients showed no alterations in their ability to ingest an ...	1989	2919528
the protective mechanism of thyroidectomy in a rat model of chronic renal failure.	selective thyroidectomy (tx) has been shown to attenuate proteinuria and disease progression in models of chronic renal failure (crf). in this investigation, four groups of munich-wistar rats were studied to determine if glomerular dynamics or another renal metabolic consequence of tx was responsible for the protective effect as measured by 24-hour protein excretion (uprot). the groups were txt4, thyroxine-replaced tx rats with five-sixths nephrectomy; tx, tx rats not receiving replacement thyro ...	1989	2919601
thyroid hormone resistance in a large kindred: physiologic, biochemical, pharmacologic, and neuropsychologic studies.	thyroid hormone resistance affects the pituitary gland and a variety of other tissues. we studied a large kindred with this disorder and measured a number of clinical markers of tissue metabolism to determine if these markers were useful in elucidating the sites and degree of resistance.	1989	2919610
doxacurium chloride for neuromuscular blockade before tracheal intubation and surgery during nitrous oxide-oxygen-narcotic-enflurane anesthesia.	the neuromuscular effects of doxacurium (bw a938u) were studied in 36 patients, divided into four groups of 9 patients each, given doxacurium either 50 micrograms/kg (2 x ed95) 5 or 4 minutes or 80 micrograms/kg (3 x ed95) 4 or 3 minutes before tracheal intubation. adequate neuromuscular relaxation permitted successful intubation at 5 minutes for doxacurium 50 micrograms/kg and at 4 minutes for 80 micrograms/kg. time to 90% blockade was 5.4 +/- 1.5 minutes for doxacurium 50 micrograms/kg and 3.5 ...	1989	2919763
selection criteria for local excision with or without adjuvant radiation therapy for rectal cancer.	as an alternative to radical surgery, local excision with or without adjuvant pelvic radiation therapy has been used in selected patients with rectal cancer. to determine which clinical and pathologic features can predict the presence of positive lymph nodes (ln+), a retrospective review of 168 patients who underwent potentially curative surgery for rectosigmoid and rectal cancer was performed. by univariate analysis, tumor penetration, grade, and histology were significant predictive features. ...	1989	2920368
the effect of severe starvation and captivity stress on plasma thyroxine and triiodothyronine concentrations in an antarctic bird (emperor penguin).	the effect of confinement and severe starvation on the plasma thyroxine (t4) and triiodothyronine (t3) concentrations was determined in emperor penguins (aptenodytes forsteri). during their annual cycle, emperor penguins fast freely for periods of up to 4 months and may thus represent a unique subject to study endocrine adaptations to fasting. plasma t4 concentrations progressively decreased following capture and confinement of naturally fasting penguins, and within 15-20 days stabilized at leve ...	1989	2920894
oxidative metabolism in a teleost, anabas testudineus bloch: effect of thyroid hormones on hepatic enzyme activities.	in vivo administration of l-thyroxine (l-t4) in anabas testudineus, while significantly stimulated the activities of cytochrome c oxidase and alpha-glycerophosphate dehydrogenase (alpha-gpdh), inhibited glucose-6-phosphate dehydrogenase (g-6-pdh), cytosolic and mitochondrial malate dehydrogenase (cyt. mdh; mit. mdh), and mg2+ dnp-dependent adenosine triphosphatase (mg2+ atpase) activities. the activities of lactate dehydrogenase (ldh), succinate dehydrogenase (sdh), and catalase remained unalter ...	1989	2920903
anti-triiodothyronine antibodies associated with hypothyroidism and lymphocytic thyroiditis in a dog.	a 5-year-old spayed shetland sheepdog had clinical signs consistent with hypothyroidism. laboratory findings, including subnormal serum t4 concentration, also were suggestive of hypothyroidism; however, the apparent serum t3 concentration, as determined by a solid phase assay, was extremely high. thyroid scintigraphy revealed ectopic radionuclide uptake in the neck and thyroid biopsy revealed lymphocytic thyroiditis. determination of t3 autoantibody titer confirmed that the previously measured h ...	1989	2921203
thyroid peroxidase activity-inhibiting immunoglobulins in patients with autoimmune thyroid disease.	the thyroid microsomal antibody (m-ab) has been found to be an antibody against thyroid peroxidase (tpo), and such antibodies have been reported not only to bind tpo but also to directly inhibit tpo activity. in this study we investigated the relationship between tpo activity-inhibiting immunoglobulin (tpii) and thyroid function in 55 untreated patients with hyperthyroidism due to graves' disease and 35 untreated patients with hashimoto's disease. tpo partially purified from the microsomal fract ...	1989	2921308
lymphocyte subsets alteration in patients with argentine hemorrhagic fever.	peripheral blood lymphocyte subpopulations were studied in 15 patients with argentine hemorrhagic fever (ahf), during the acute period of the disease and in early convalescence. anti-human ig antibodies were used to identify b cells and monoclonal antibodies to assess t4 and t8 subsets. during the acute period of the disease, significant alterations were found in b, t4, and t8 lymphocytes (p less than .001), as well as in t4/t8 ratios (p less than .001). these abnormalities disappeared in early ...	1989	2921602
effect of dietary iodine on autoimmune thyroiditis in the bb wistar rats.	diabetes-prone bb wistar rats were fed a modified ain-76 diet providing the following amounts of iodine for 10 wk: 0.2 mg/kg diet (recommended amount); 1.0 mg/kg; 2.0 mg/kg; or 3.0 mg/kg. the thyroids were examined for gross and microscopic changes and sera were assayed for antibodies to triiodothyronine (t3), thyroxine (t4), and thyroglobulin (tg). the body weights and food consumption of the rats fed 0.2 mg of iodine/kg were significantly lower than those of the animals fed higher amounts. uri ...	1989	2921647
carcinoma of the nasopharynx--comparison of the uicc and ho clinical staging systems.	from 1974 to 1985 definitive radiation therapy was performed in 76 patients with nasopharyngeal carcinoma (npc). of the 76 patients 59 presented uicc stage iv and 13 uicc stage iii. the distribution of the stages was more homogeneous for the ho system: ho i, n = 4; ii, n = 21; iii, n = 16; iv, n = 32; v, n = 3. radiotherapy followed the guidelines of the m.d. anderson hospital in houston. a median dose of 68 gy was given to the primary and of 66 gy to the neck nodes, independent of the histologi ...	1989	2921845
effects of vip and helodermin on thyroid hormone secretion in the mouse.	the two peptides vip (vasoactive intestinal peptide) and helodermin have both been shown to occur within the thyroid gland: vip in intrathyroidal nerves and helodermin in the c-cells. both peptides have previously been demonstrated to enhance the release of radioiodine from the prelabelled thyroid in vivo. since a considerable amount of radioiodine released from the thyroid under these conditions may be non-hormonal, we reexamined the effects of vip and helodermin on thyroid hormone secretion by ...	1989	2922106
energy cost of paraplegic locomotion with the orlau parawalker.	ten subjects with neurological lesion levels between t4 and t9 have been evaluated in the laboratory at orlau using their orthosis in its mechanical form alone (without fes assist). initially the oxygen consumption at rest was measured. then the patients were asked to walk (orthotic gait) at their preferred constant steady speed for a period of 5 minutes. min. vo2 was measured during the exercise. from these readings the energy cost and consumption of the patients' gait was calculated. our resul ...	1989	2922207
unusual chronic maculopapular rash associated with human immunodeficiency virus infection.	a 21-year-old black man with sickle cell disease who has received blood transfusions for 11 years because of recurrent stroke has had a decline in numbers of circulating lymphocytes and t4 cells over the past four years. two and a half years ago persistent generalized lymphadenopathy developed, associated with a positive test for human immunodeficiency virus (hiv) antibody. for the past seven months he has had a pruritic maculopapular rash, primarily on the extensor surfaces of the extremities. ...	1989	2922631
the value of morphometry and dna flow cytometry in addition to classic prognosticators in superficial urinary bladder carcinoma.	in 80 patients with primary superficial bladder carcinoma tumor nodes metastasis (tnm classification: stages ta and t1) with adequate follow-up of at least four years, the value of selective nuclear morphometry and dna flow cytometry on paraffin-embedded material in addition to classic prognosticators was assessed. only the quantitative techniques appeared to be valuable predictors of new tumor occurrence. the recurrence rate in patients with large nuclei (mean nuclear area greater than 95 micro ...	1989	2923091
coil selection for magnetic resonance imaging of the cervical and thoracic spine using a vertical magnetic field.	in order to optimize the coil selection for cervical and thoracic spine imaging the signal characteristics of two different solenoidal surface coils (15 cm and 30 cm diameter, respectively) as well as the head coil and body coil were determined using a 0.3 t mr scanner with a vertical magnetic field. signal-to-noise ratio curves were obtained for each coil using tube phantoms and a human-like phantom. the findings were compared with images obtained in two healthy volunteers. the head coil was fo ...	1989	2923735
[chronic paranoid-hallucinatory psychosis as the initial manifestation of an hiv infection?].	in a 36-year-old patient an acute onset of psychosis occurred, probably due to hiv infection. for one year hiv-infection with reduced t4/t8 ratio had been known without clinical manifestation (stage iv b of the cdc-classification). he developed chronic delusional hallucinations, which persisted for more than one year in spite of adequate psychoactive drug therapy. so far aids-related dementia has not become evident. focal lesions caused by opportunistic infections or tumour were excluded by comp ...	1989	2924704
[interhormonal relations in the dynamics of immune response during thyroidectomy].	wistar rats were immunized with the sheep erythrocytes (se) within 18 or 60 days after thyroidectomy. the latter led to a sharp drop in concentration of t3, t4 and to 2-3-fold increase in the tth level. the se administration failed to induce an increase in the t3, t4 level in operated animals whereas the tth concentration increased. during the inductive period of immunogenesis a reduction occurred in the antigen-induced glycocorticoid response and the testosterone level in the early period repla ...	1989	2924963
seasonal changes in body weight, fat depots, and plasma levels of thyroxine and growth hormone in free-living great tits (parus major) and willow tits (p. montanus).	annual changes in body weight, fat depots, and plasma levels of thyroxine (t4) and growth hormone (gh) were studied in free-living great tits and willow tits. birds were collected during six ecologically well-defined periods of the year. special attention was given to the nonreproductive part of the year. t4 showed simple unimodal cycles in both species and both sexes, with high levels during the warmer part of the year, and low levels during the winter and spring periods. although increasing le ...	1989	2925079
corticosteroid receptors in liver cytosol of the clawed toad, xenopus laevis: influence of thyroid and ovarian hormones.	the glucocorticoid receptor capacity ro and the dissociation constant kd were determined in the liver of xenopus laevis by scatchard analysis. in 5-year-old female toads ro was about three times higher than that in males (153.9, 54.3 fmol/mg protein) and kd was similar in both sexes (4.0, 4.1 nm). some of the animals used had abnormal enlarged thyroid glands, atrophic ovaries, or both defects in connection with different levels of ro, but not of kd, compared to those of normal animals. females w ...	1989	2925084
macrophages and interdigitating reticulum cells in normal thymus and in thymoma: an immunohistochemical study.	the distribution and immunophenotype of macrophages and interdigitating reticulum cells were investigated on frozen sections of seven normal thymuses and 10 thymomas. in normal thymus, macrophages were mainly located in the cortex, were markedly pam-1+/mac+, weakly leu-m3+ (cd14), t4+ (cd4), t9+ and okm-1+ (cd11b). interdigitating reticulum cells were mainly located in the medulla and were pan-leu+ (cd45), t4+(cd4+), hla-dr+; furthermore, they were also often tac+ (cd25) and t9+. thymomas were c ...	1989	2925178
the contingent negative variation in a go/no go avoidance task: relationships with personality and subjective state.	howard et al. (1982) reported an association in mentally abnormal offenders between psychometric impulsiveness and the degree of differentiation in the contingent negative variation (cnv) recorded at the vertex between go and no go conditions in a go/no go signalled avoidance task. the present study aimed to investigate whether this finding extended to two samples of young, healthy volunteers using a variety of impulsiveness-related measures. as well as examining the go/no go cnv recorded from t ...	1989	2925463
effect of exogenous thyroxine on the interval from weaning to estrus of the primiparous sow.	a total of 159 primiparous sows were fed 0 (c) or 675 mg/d of thyroprotein (tp) from d -2 until d 14 postweaning. sows received 8 (le) or 14 (he) mcal of me/d during a 28-d lactation period. plasma levels of 3,5,3'-triiodothyronine (t3) and 3,5,3',5'-tetraidothyronine (t4) were determined for d -4, -2, 0, 1, 2, 3, 5 and 7 postweaning. sows consuming le lost more weight and backfat during lactation (p less than .01) than he sows. litters of sows consuming le gained less weight than litters of sow ...	1989	2925539
stimulation in vitro of rabbit erythrocyte cytosol phospholipid-dependent protein kinase activity. a novel action of thyroid hormone.	l-thyroxine (t4) and 3,3',5-l-triiodothyronine (t3) at 10(-10) m stimulated phospholipid- and ca2+-dependent protein kinase activity in rabbit red cell cytosol in vitro by 151 and 176%, respectively. kinase of 30-fold greater specific activity, developed with 0.4 mm nacl from cytosol applied to deae-cellulose, was also stimulated up to 2-fold by thyroid hormone. hormone enhancement of kinase activity occurred after 60 min of incubation at 37 degrees c prior to enzyme assay. thyroid hormone analo ...	1989	2925667
radioactive iodine treatment of a functional thyroid carcinoma producing hyperthyroidism in a dog.	radioactive iodine (131i) was used in the treatment of a 12-year-old female dog with hyperthyroidism resulting from a large, unresectable (and metastatic) thyroid carcinoma associated with signs of severe inspiratory stridor and dyspnea. hyperthyroidism was diagnosed on the basis of clinical signs (polyuria, polydipsia, polyphagia, weight loss, nervousness) and high basal serum thyroxine (t4) concentrations, as well as thyroid radioiodine kinetic studies that showed a high radioiodine uptake int ...	1989	2926718
neoadjuvant treatment of stages t2 to t4 bladder cancer with cis-platinum, cyclophosphamide and doxorubicin.	in an ongoing phase ii study 17 patients with potentially operable transitional cell carcinoma of the bladder (stages t2 to t4, nx, mo) have been treated with intravenous cis-platinum (50 mg.per m.2), cyclophosphamide (400 mg.per m.2) and doxorubicin (40 mg.per m.2). they were to receive 3 treatments at 3-week intervals before cystectomy and 2 treatments at 3-week intervals commencing 5 weeks after cystectomy. of 17 patients 14 (82 per cent) completed all 3 preoperative treatments but only 7 (41 ...	1989	2926878
replication of dna minicircles in kinetoplasts isolated from crithidia fasciculata: structure of nascent minicircles.	we have previously described an isolated kinetoplast system from crithidia fasciculata capable of atp-dependent replication of kinetoplast dna minicircles (l. birkenmeyer and d.s. ray, j. biol. chem. 261: 2362-2368, 1986). we present here the identification of two new minicircle species observed in short pulse-labeling experiments in this system. the earliest labeled minicircle species (component a) contains both nascent h and l strands and is heterogeneous in sedimentation and electrophoretic m ...	1989	2927390
host cell reactivation of cat-expression vectors as a method to assay for cloned dna-repair genes.	we demonstrate the feasibility of using passive host-cell reactivation of a shuttle-vector prsvcat to detect cloned dna-repair genes. as models, a transient expression vector, prsvdenv, and a positive-selection vector, prsvdenv/svgpt, were constructed containing the t4 coliphage denv gene, coding for an ultraviolet-specific endonuclease, under promotion of the rous sarcoma virus (rsv) long-terminal repeat. cotransfection of one or three copies of prsvdenv per uv-irradiated prsvcat molecule into ...	1989	2927423
[pre- and postoperative radiotherapy of oral carcinoma of a locally advanced stage. an analysis of the results and complications].	the combination of radiotherapy and surgery in the treatment of advanced oral carcinoma (t3 and t4 lesions) yields good possibilities of recovery; whether radiotherapy should be given before or after surgery is still debated. fifty patients with advanced oral carcinomas were analyzed: 24 of them were irradiated before and 26 after surgery; doses ranged from 40 to 56 gy for the first group of patients, and from 50 to 68 gy for the second one. the disease-free survival 48 months after the diagnosi ...	1989	2928571
iodine nutrition in ewes: effects of low to high iodine intake on iodine content of biological fluids in pregnant and lactating ewes.	in a first experiment, 2 groups of 46 and 47 multiparous ewes received diets which provided an iodine intake of 0.36 and 0.26 mg/kg dry matter (group c) and, 2.01 and 1.94 mg/kg (group d), respectively, for pregnancy and lactation. in a second experiment, 3 groups of 10 nulliparous ewes received diets which provided an i intake of 0.13 and 0.12 mg/kg dry matter (group a), 0.22 and 0.20 mg/kg dry matter (group b), and 10.77 and 8.88 mg/kg dry matter (group e), respectively, for pregnancy and lact ...	1989	2928599
pituitary resistance to l-thyroxine after thyroidectomy. a case report.	the clinical and laboratory findings in a 29-year-old saudi arabian woman were compatible with a state of resistance to thyroid hormones by the pituitary gland. symptoms of hypothyroidism, amenorrhoea and secondary infertility developed 10 years after partial thyroidectomy for an euthyroid goitre. before therapy, serum thyroxine (t4) and thyroid-stimulating hormone (tsh) values were elevated; serum tri-iodothyronine (t3) value was at the upper limit of normal while %t3 resin uptake and serum fre ...	1989	2928862
acute thyroid crisis on induction of anaesthesia.	thyroid crisis on induction of anaesthesia was treated with dantrolene, because of a mistaken diagnosis of malignant hyperthermia. there was immediate improvement after dantrolene with reduction in muscle rigidity, mental confusion and pyrexia. high circulating t4 has an effect on calcium flux across the sarcoplasmic reticulum and dantrolene may inhibit this pathological mechanism. we suggest the same dosage regimen as is used in the treatment of malignant hyperthermia.	1989	2929904
dna polymerase alpha activity is not affected by protein kinases or alkaline phosphatase.	recent studies with crude or partially purified cell extracts have suggested that dna polymerase alpha activity may be regulated by enzymatic phosphorylation. to further investigate these findings, we have examined the effects of protein kinases and phosphatases on highly purified dna polymerase alpha from mouse cells. incubation of dna polymerase alpha with a variety of protein kinases, including protein kinase c, had no effect on polymerase activity. in addition, treatment of the polymerase wi ...	1989	2930569
several hypothalamic peptides stimulate in vitro thyrotropin secretion by pituitaries of anuran amphibians.	the effects of several hypothalamic peptides on hormone secretion by pituitaries of three species of anuran amphibians were investigated using in vitro techniques. secretion of thyrotropic bioactivity (designated thyrotropin or tsh) was quantified by bioassay of the pituitary incubation medium using thyroxine (t4) production by paired thyroids from the same animals. pituitaries from adult male rana pipiens were cultured in medium alone, 10 or 100 ng/ml thyrotropin-releasing hormone (trh), 1000 n ...	1988	2853681
train-of-four fade during neuromuscular blockade induced by tubocurarine, succinylcholine or alpha-bungarotoxin in the rat isolated hemidiaphragm.	1. nerve-evoked maximal twitches (t1, t2, t3, t4) of the rat isolated hemidiaphragm to train-of-four (tof) stimulation (2 hz x 2 s) were recorded continuously in the absence or presence of tubocurarine (1.5 mumol/l), succinylcholine (40 mumol/l) or alpha-bungarotoxin (1 mumol/l). the t1 and t4 response-time profiles for the three drugs were analysed with respect to amplitude depression and the tof ratio (t4/t1) during the development of and recovery from neuromuscular blockade. 2. tubocurarine p ...	1988	2854017
effect of methimazole-induced hypothyroidism on multiple opioid receptors in rat brain regions.	the effect of chronic administration of methimazole (0.05% w/v) in drinking water for 32 days to male sprague-dawley rats on the binding of opioid ligands, 3h-tyr-d-ala-gly-mephe-gly-ol (dago, mu-receptors), 3h-tyr-d-ser-gly-phe-leu-thr (dstle, delta-receptors) and 3h-ethylketocyclazocine (ekc, kappa-receptors) to membranes of brain regions was determined. chronic administration of methimazole to rats decreased their rate of body weight gain, colonic temperature, systolic blood pressure and hear ...	1988	2854273
ultrastructure of human retroviruses.	we compared the ultrastructure of the human retroviruses by thin-section electron microscopy of infected lymphocytes. virus particles form at the plasma membrane without involvement of a cytoplasmic precursor. budding forms of human t-cell leukemia virus types i and ii (htlv-i and -ii) consist of a crescent-shaped nucleoid separated from the envelope by an intermediate layer. mature forms of these viruses are about 100 nm in diameter. the nucleoid is electron lucent and almost completely fills t ...	1988	2854553
[plasma exchange with very low molecular weight heparin cy 222. biological profile and therapeutic value].	in a clinical, between-patient study we investigated the effects of a vlmw heparin fragment (cy 222) versus standard heparin (sh) in plasma exchanges (n = 10) on coagulation factors (cf). fibrinogen (fgn), ii, v, viif + x, ix, xi, xii, viiic, viiirag, viiivwf and atiii, protc, prots, plasminogen (pgn), activated thromboplastin time (aptt), prothrombin time (pt), thrombin time (tt), anti factor xa (axa), ddimer, platelet count. heparin was administered as a bolus and by infusion during the sessio ...	1988	2854713
[nucleotide sequence of gene 31 of the t4 bacteriophage].	we have determined the nucleotide sequence of a region of 656 nucleotides comprising the 31 gene of bacteriophage t4. the coding region consisted of 333 nucleotides directing the synthesis of a polypeptide of 111 amino acids, with a calculated molecular weight of 12,060. the upstream sequence contains the consensus sequences for t4 early and two middle promoters. the downstream sequence contains the consensus sequence for t4 late promoter and the inverted repeats. in addition, there are two inco ...	1988	2855254
identification of nanopeptide from htlv-iii, arv-2 and lavbru envelope gp120 determining binding to t4 cell surface protein.	the information contained in the primary structure of hiv envelope gp120 determining its biological activity, was analyzed by the use of the informational spectrum method. the parameter determining the interaction between gp120 and t4 cell-surface protein was defined. on the basis of this data in the conserved region of gp120 from htlv-iii, lavbru and arv-2, a nanopeptide naktiivql was identified as a potential binding domain which could be used as a component of an effective therapeutic agent o ...	1988	2855309
spectrum of thyroid function abnormalities in gestational trophoblastic neoplasia.	the relative influences of the putative thyroid stimulator, pregnancy state and non-thyroidal illnesses, were assessed in 52 patients with gestational trophoblastic neoplasia (gtn). in this study biochemical thyroid status was assessed with the aid of thyrotrophin (tsh) measurements by a sensitive immunoradiometric assay and measurement of total and free thyroid hormone concentrations. the reference group consisted of 63 patients with normal pregnancy. thyroid function in the gtn patients was al ...	1988	2855724
the effect of thyroidectomy in the fetal sheep on lung liquid reabsorption induced by adrenaline or cyclic amp.	1. in fetal sheep at 113-120 days' gestation, thyroidectomy was performed and tracheal, arterial and venous catheters inserted. following a recovery period experiments were performed from 120-145 days to measure changes in lung liquid secretion or its absorption in response to i.v. adrenaline infusion or to introduction of dibuteryl cyclic amp into lung liquid. the results were compared with those previously obtained in non-thyroidectomized fetuses. 2. plasma levels of thyroid hormones in non-th ...	1988	2855741
tsh-receptor antibodies in mothers with graves' disease and outcome in their offspring.	blood was taken from 56 selected newborn babies whose mothers had graves' disease, to assess the relation between their thyroid function and the presence of thyrotropin (tsh) binding inhibitor immunoglobulins (tbii) and thyroid stimulating antibodies (tsab) in maternal serum. all the mothers of the thyrotoxic babies had both these tsh receptor antibodies in their serum. however, most of the mothers whose thyroid function had been well controlled in pregnancy gave birth to normal babies. 15 babie ...	1988	2891888
phase i studies of 2',3'-dideoxycytidine in severe human immunodeficiency virus infection as a single agent and alternating with zidovudine (azt).	five dose regimens of 2',3'-dideoxycytidine (ddc) were administered, intravenously for 2 weeks then orally for 4 or more weeks, to 20 patients with acquired immunodeficiency syndrome (aids) or aids-related complex (arc). ddc was well absorbed from the gut and crossed the blood-brain barrier. 10 of the 15 patients who received 0.03-0.09 mg/kg every 4 h had increases in their absolute number of t4+ t cells at week 2 (p less than 0.05), though in many these rises were not sustained. 11 of 13 evalua ...	1988	2891981
[graves' disease in childhood].	authors describe clinic and therapeutic results in seven hyperthyroid graves' disease patients between ages of three and a half an 15 and a half (x: 11.2) years relating their results with those of literature. most peculiar aspects of these cases were pretibial myxedema in two patients and lack of goiter in one of them. results include t4 greater than 18.5 mcg/100 ml in five, t3 greater than 645 ng/100 in three and a relationship between t3/t4 greater than or equal to 20 in five. these results h ...	1988	2469369
thyroxine, renal beta-adrenergic receptors, and dipsogenesis in food-deprived rats.	the effect of thyroxine (t4) replacement on the increased renal beta-adrenergic receptor number and the increased beta-adrenergic dipsogenic responsiveness of fasted rats was studied in male sprague-dawley rats. food deprivation significantly decreased serum thyroxine (t4) and triiodothyronine (t3) levels, increased the dipsogenic response to isoproterenol, and elevated renal beta-adrenergic receptor concentration. daily administration of t4 (40 micrograms/kg) to food-deprived rats restored seru ...	1988	2827530
production of phagocytosis-inducing factor and expression of 4b4 antigen by cloned human t cells before and after transformation with htlv-i.	the characteristics of 4 t-cell clones, each capable of producing phagocytosis-inducing factor (pif), were compared before and after transformation with human t-lymphotropic virus type 1 (htlv-i). before transformation, the four clones produced pif transiently after stimulation with antigen or mitogen and expressed the phenotype t3(cd3)+, t4(cd4)+, t8(cd8)-, 4b4+, and 2h4-; the three clones that could be studied also expressed the okt17 marker. after transformation, the cells expressed the same ...	1988	2827893
further purification and characterization of the dna 3'-phosphatase from rat-liver chromatin which is also a polynucleotide 5'-hydroxyl kinase.	the dna 3'-phosphatase activity of rat-liver chromatin has been purified. a dna 5'-hydroxyl kinase activity comigrates at each step of purification. both enzymes have the same molecular mass (79 kda) and the same isoelectric point (8.6). it thus seems that the two activities are born by the same protein just as with the phage t4 enzyme which is, at the same time, a 5'-hydroxyl kinase and a 3'-phosphatase. an additional argument is that atp, which does not influence the rate of the 3'-phosphatase ...	1988	2828058
molecular complexes of thyroid hormone tyrosyl rings with aromatic donors. possible relationship to receptor protein interactions.	several lines of evidence have indicated that thyroid hormones share common molecular properties (accessible planar face and lateral halogenation) with 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) and related compounds of environmental importance and can modulate their toxicity. binding of dioxin to a soluble intracellular protein (dioxin or ah receptor) appears to be the initial step in their mechanism of toxicity and a stacking interaction model has been proposed at the molecular level. it has a ...	1988	2828621
hiv infection is blocked in vitro by recombinant soluble cd4.	the t-cell surface glycoprotein, cd4 (t4), acts as the cellular receptor for human immunodeficiency virus, type 1 (hiv-1), the first member of the family of viruses that cause acquired immunodeficiency syndrome. hiv recognition of cd4 is probably mediated through the virus envelope glycoprotein (gp120) as shown by co-immunoprecipitation of cd4 and gp120 (ref.5) and by experiments using recombinant gp120 as a binding probe. here we demonstrate that recombinant soluble cd4(rst4) purified from the ...	1988	2829022
a soluble cd4 protein selectively inhibits hiv replication and syncytium formation.	the cd4 (t4) molecule is expressed on a subset of t lymphocytes involved in class ii mhc recognition, and is probably the physiological receptor for one or more monomorphic regions of class ii mhc (refs 1-3). cd4 also functions as a receptor for the human immunodeficiency virus (hiv) exterior envelope glycoprotein (gp120) (refs 4-9), being essential for virus entry into the host cell and for membrane fusion, which contributes to cell-to-cell transmission of the virus and to its cytopathic effect ...	1988	2829023
bacteriophage t2 and t4, dam+ and damh and eco dam+ methylation: preference at different sites.	we present a method for determining preference for methylation at minor methylation sites. the target dna sequence is first subjected to computer-assisted analysis to predict which restriction endonuclease(s) will generate fragments that will contain only one or two likely minor methylation site(s). the target dna is then methylated in vitro with a radioactive methyl-group donor and subjected to digestion by the chosen restriction enzyme(s). the amount of radioactivity in the various fragments i ...	1988	2829967