Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| etiology of childhood hypothyroidism. | this study prospective without any selection bias included 80 of the 152 hypothyroid infants and children seen over the past six years. the clinical diagnosis was confirmed by tsh and thyroid hormone (t3, t4) studies. scanning for thyroid with tc99m pertechnetate was carried out in all except seven older children with grade ii and iii goiters where 131i uptake studies were done. serum thyroglobulin (ria) was estimated and antithyroglobulin and antimicrosomal antibodies were tested. based on thyr ... | 1989 | 2546895 |
| common sites for recombination and cleavage mediated by bacteriophage t4 dna topoisomerase in vitro. | we have previously shown that purified t4 dna topoisomerase promotes illegitimate recombination between two lambda dna molecules, or between lambda and plasmid dna in vitro (ikeda, h. (1986) proc. natl. acad. sci. u. s. a. 83, 922-926). since the recombinant dna contains a duplication or deletion, it is inferred that the cross-overs take place between nonhomologous sequences of lambda dna. in this paper, we have examined the sequences of the recombination junctions produced by the recombination ... | 1989 | 2546934 |
| two early genes of bacteriophage t5 encode proteins containing an ntp-binding sequence motif and probably involved in dna replication, recombination and repair. | it is demonstrated, by computer-assisted analysis, that t5 bacteriophage early genes d10 and d13 encode proteins containing the purine ntp-binding sequence motif. the d10 gene product is shown to be a member of a recently characterized superfamily of (putative) dna and rna helicases. the d13 gene product is related at a statistically significant level, to the gene 46 product of bacteriophage t4 which is a component of an exonuclease involved in phage dna replication, recombination and repair. a ... | 1989 | 2547651 |
| [the clinical value of computed tomography in the pretherapeutic t-staging of orofacial tumors]. | the clinical role of contrast-enhanced ct for pre-operative t-staging of oro-facial tumours was evaluated in 174 patients. during stages t1 and t2, clinical examination is 90% accurate and has a significantly higher sensitivity than ct, with an accuracy of only 53%. the clinical diagnosis of t1 and t2 is, however, made with undue frequency; ct makes it possible to obtain correct staging. combination of ct and clinical examination can improve correct staging from 46% to 81%. during stages t3 and ... | 1989 | 2548237 |
| tissue-specific isoform regulation of na+-k+-atpase by thyroid hormone in ferrets. | we have shown previously that in the ferret heart there are two isoforms of na+-k+-atpase, alpha(+) and alpha, and that these isoforms undergo developmental changes. in the present study, we examine regulation of the isoenzymes by thyroid hormone, which is well known to increase activity of na+-k+-atpase in different tissue preparations. ferrets were injected with l-thyroxine (t4) (0.5 mg/kg, sc) for 3 wk. the t4-treated ferrets gained 58 +/- 32 g body wt compared with 366 +/- 24 g for the contr ... | 1989 | 2548405 |
| comparative x-ray crystallographic evidence for a beta-bend conformation as the active structure for peptide t in t4 receptor recognition. | a sequence similarity has been found between two segments of endothiapepsin (acid proteinase, 2ape), bovine pancreatic ribonuclease a, and peptide t, a segment of the gp120 protein of human immune deficiency virus (hiv), which has been implicated in blocking viral attachment to the t4 receptor. the two similar sequences of the acid proteinase enzyme are leu-ile-asp-ser-ser-ala-tyr-thr (residues 169-176) and tyr-thr-gly-ser-leu-asn-tyr-thr (residues 175-182). since the x-ray crystallographic stru ... | 1989 | 2548525 |
| cns cell groups regulating the sympathetic outflow to adrenal gland as revealed by transneuronal cell body labeling with pseudorabies virus. | the cns cell groups that innervate the sympathoadrenal preganglionic neurons of rats were identified by a transneuronal viral cell body labeling technique combined with neurotransmitter immunohistochemistry. pseudorabies virus was injected into the adrenal gland. this resulted in retrograde viral infections of the ipsilateral sympathetic preganglionic neurons (t4-t13) and caused retrograde transneuronal cell body infections in 5 areas of the brain: the caudal raphe nuclei, ventromedial medulla, ... | 1989 | 2548665 |
| ring-opened 7-methylguanine nucleotides are resistant to nuclease p1 digestion and good substrates to polynucleotide kinase. | dimethyl sulfate was used to prepare 7-methyl-2'-deoxy-guanosine 3'-monophosphate (7-methyl-dgmp), which was ring-opened in alkali to 2'-deoxy-n5-methyl-n5-formyl-2,5,6-triamino-4-oxopyrimidine 3'-monophosphate (rom-dgmp). rom-dgmp was not dephosphorylated by nuclease p1 in contrast to normal deoxynucleotides. it was efficiently 5'-phosphorylated by t4 polynucleotide kinase. when methylated dna was alkali-treated and digested with micrococcal nuclease, spleen phosphodiesterase and nuclease p1, r ... | 1989 | 2548753 |
| using transposon tn5 insertions to sequence bacteriophage t4 gene 11. | a simple and rapid method of creating an overlapping set of deletions in cloned dna in preparation for sequencing has been developed. the method is based on a positive selection for tn5 transposition into the cloned dna fragment on a high-copy-number filamid, resolution of potential filamid dimers by filamentous phage infection, and the use of tn5 both as a "portable" restriction enzyme site for in vitro dna deletion and a "portable" sequencing primer binding site to initiate dna sequencing reac ... | 1989 | 2548819 |
| [an autopsy case of aids with disseminated cytomegalovirus infection and neurological disturbance]. | we report a case of acquired immunodeficiency syndrome (aids) complicated by disseminated cmv infection and neurological disturbance. a 21 years old male with hemophilia a was diagnosed as having aids in feb. 1986 because of interstitial pneumonia and esophageal candidiasis. since jan. 1987 he had complained of hypesthesia in the legs. on mar. 14 he was admitted due to diarrhea. the laboratory data revealed that wbc was 4,000/microliters including 29% of lymphocytes, 1.6% of okt4+-, 71.6% of okt ... | 1989 | 2549283 |
| characterization of four herbicide-resistant mutants of rhodopseudomonas viridis by genetic analysis, electron paramagnetic resonance, and optical spectroscopy. | herbicides of the triazine class block electron transfer in the photosynthetic reaction centers of purple bacteria and psii of higher plants. they are thought to act by competing with one of the electron acceptors, the secondary quinone, qb, for its binding site. several mutants of the purple bacterium rhodopseudomonas viridis resistant to terbutryn [2-(methylthio)-4-(ethylamino)-6-(tert-butylamino)-s-triazine] have been isolated by their ability to grow photosynthetically in the presence of the ... | 1989 | 2550055 |
| the phage t4 uvs y recombination protein stabilizes presynaptic filaments. | the bacteriophage t4 uvsy protein is required for efficient recombination in t4-infected escherichia coli cells. previous in vitro work has shown that the purified uvsy protein is an accessory protein; it stimulates homologous pairing catalyzed by the phage uvsx protein (a reca-like recombinase) under certain conditions. we show here that this effect can be traced, at least in part, to a uvsy-dependent stabilization of uvsx protein-single-stranded dna complexes. these presynaptic filaments are o ... | 1989 | 2550444 |
| effects of epidermal growth factor on thyroglobulin and adenosine 3',5'-monophosphate production by cultured human thyrocytes. | while several workers have identified epidermal growth factor (egf) receptors on human thyroid membranes, very few reports have described egf effects on intact human thyroid cells in primary culture, and these were short term studies indicating that egf effects were primarily inhibitory [reduced iodide uptake and thyroglobulin (tg), t4, and t3 release]. paradoxically, in vivo egf stimulates thyroid growth and increases colloid stores. in this study we examined the effects of egf on cultured thyr ... | 1989 | 2550506 |
| thyroid-stimulating activity in sera of normal pregnant women. | to evaluate the effect of a thyroid stimulator on thyroid function in the sera of normal pregnant women, we measured thyroid-stimulating activity (tsa) using a highly sensitive bioassay based on camp accumulation in cultured rat frtl-5 thyroid cells. serum was pretreated with 10% polyethylene glycol (peg), and the supernatant (peg-pretreated serum) was then used in the following studies. frtl-5 cells were preincubated in 5h medium and incubated for 2 h with peg pretreated serum, and camp was mea ... | 1989 | 2550510 |
| harderian glands of golden hamsters: morphological and biochemical responses to thyroid hormones. | manipulation of circulating levels of thyroid hormones modifies harderian gland structure and porphyrin concentrations in male and female golden hamsters. specifically, thyroxine (t4) and triiodothyronine (t3) induce the morphological conversion of the harderian glands of females to approximate those of the male. further, porphyrin concentrations are markedly decreased by this treatment. this effect occurs in ovariectomized animals as well, indicating that the gonads are not involved. suppressio ... | 1989 | 2550527 |
| characterization of tumor necrosis factor-alpha receptors in human and rat thyroid cells and regulation of the receptors by thyrotropin. | administration of recombinant human tumor necrosis factor-alpha (tnf) to rats and mice produces a model of nonthyroid illness in which there is impairment of hypothalamic-pituitary thyroid function, including reduced serum concentrations of t4 and t3, reduced thyroid radioiodine uptake, and reduced response to tsh. in this study, we tested the binding and effects of tnf on frtl-5 cells and on four human thyroid carcinoma cell lines. the tsh-stimulated [125i]iodide uptake by frtl-5 cells was inhi ... | 1989 | 2551628 |
| stimulation of the chlorinating activity of human myeloperoxidase by thyroid hormones and analogues. | thyroxine and triiodothyronine concentrations of 50 nm or lower can stimulate the chlorinating activity of the myeloperoxidase-h2o2-cl- antimicrobial system in vitro. the initial rates of the chlorinating reaction with monochlorodimedone were similar for both thyroid hormones. maximum stimulation occurred around ph 6 and a linear relationship exists between stimulation and thyroxine concentrations up to at least 1 microm. of the various thyroxine analogues tested, stimulation was in the order: t ... | 1989 | 2551809 |
| modulation of the neonatal pituitary and adrenocortical responses to stress by thyroid hormones in the rat: effects of hypothyroidism and hyperthyroidism. | neonatal rats exhibit a period of diminished pituitary and adrenocortical responses to stress during the first 2 weeks of life. since thyroid hormones are known to affect brain development, modulation of these responses to stress by alterations in thyroid hormone status have been investigated in hypothyroid (hypo) and hyperthyroid (hyper) rat pups. changes in acth and corticosterone (b) levels were measured under basal and stress conditions (3 min exposure to ether vapors) in neonates of various ... | 1989 | 2552340 |
| [role of sex dimorphism in the pharmacological action of etimizol]. | it has been shown that in female rats the level of acth, corticosterone in the blood, relative mass of adrenals, maintenance of t3, t4 in the serum and liver was significantly higher, but the activity of liver enzyme microsomes system was lower than in males; no sex differences were observed in myocardial creatine phosphokinase system. the influence of the etimizol on the female rats significantly speed up amidopyrine n-demethylation and biotransformation of hexobarbital. in males these systems ... | 1989 | 2553148 |
| antigen-stimulated human interferon-gamma generation: role of accessory cells and their expressed or secreted products. | in response to cytomegalovirus (cmv) and toxoplasma gondii antigens, t4+ cells from seropositive donors produce interferon-gamma (ifn-gamma) by different mechanisms; one (t. gondii) dependent upon and the other (cmv) largely independent of interleukin-2 (il-2) and its receptor. to determine whether ifn-gamma-generating mechanisms unrelated to il-2 also differ, we examined the requirement for accessory cells and their expressed or secreted products. in response to both specific antigens, ifn-gamm ... | 1989 | 2553309 |
| thyroid hormone action on acth secretion. | thyroid hormone effects on pituitary acth have not been well established. adult male sprague-dawley rats were rendered hypo- and hyperthyroid while undergoing treatment with 6-propylthiouracil (ptu) and l-thyroxine (t4). at the time of decapitation, plasma values for t4 (micrograms/100 ml) were 3.9 +/- 0.4 in the control, 17.3 +/- 2.2 in the t4 and less than 2 in the ptu treated group; plasma t3 and tsh confirmed hyper- and hypothyroidism in the t4 and ptu treated groups respectively. plasma imm ... | 1989 | 2553572 |
| molecular epidemiologic analysis of the type a streptococcal exotoxin (erythrogenic toxin) gene (spea) in clinical streptococcus pyogenes strains. | a molecular epidemiology analysis was performed with over 440 clinical isolates of streptococcus pyogenes obtained from 11 different countries in order to determine the frequency of occurrence of the type a streptococcal exotoxin (erythrogenic toxin) gene (spea) among group a strains. the colony hybridization technique employing a specific internal fragment of the spea gene was used for initial screening, and all positive results were further confirmed by the southern hybridization technique. am ... | 1989 | 2553612 |
| differential hormone-binding characteristics of thyroxine-binding globulin in hepatocellular carcinoma and cirrhosis. | to determine whether previous observations suggesting reduced ability of thyroxine-binding globulin (tbg) to bind exogenous thyroid hormones in hepatocellular carcinoma (hcc) might form the basis of a useful clinical test, we have investigated a larger series involving 49 patients and 10 healthy subjects. the binding ratio (mol hormone bound/mol tbg at a given hormone concentration) for both t4 and t3 was significantly reduced when compared to both cirrhotic and normal subjects. no overlap in t4 ... | 1989 | 2553799 |
| changes in multiple opioid receptors in regions of the brain in rats treated chronically with thyroxine. | the effect of the administration of thyroxine (t4) (1 mg/kg, s.c.), to male sprague-dawley rats on alternate days for 18 days, on the binding of opioid ligands, [3h]tyr-d-ala-gly-mephe-gly-ol (dago, mu receptors), [3h]tyr-d-ser-gly-phe-leu-thr (dstle, delta receptors) and [3h]ethylketocyclazocine (ekc, kappa receptors) to membranes of regions of the brain was determined. the chronic administration of thyroxine to rats decreased their rate of gain of body weight, increased colonic temperature and ... | 1989 | 2554187 |
| the radiology of the right superior intercostal vein. | the right superior intercostal vein is visualised on ct examination as a circular opacity laterally at the right aspect of the vertebral body at the t4-t5 level. in venographic examination the rsiv appears to be formed by the confluence of the venous channels, the right second, third and fourth intercostal veins. in superior vena cava obstruction the rsiv is an important collateral pathway: the flow through the vein depends on the site of the obstruction, anterograde if the obstruction is superi ... | 1989 | 2554413 |
| t-lymphocyte subsets in gut and blood of liver transplant recipients with and without cytomegalovirus gastroenteritis. | the effects of orthotopic liver transplantation (oltx) and cytomegalovirus (cmv) gastroenteritis on the type of mononuclear cells within the upper gastrointestinal tract were determined. nineteen liver transplant recipients were studied both before and after transplantation. each underwent a pan-upper gastrointestinal endoscopy with biopsy of the antrum and duodenum before and four weeks following liver transplantation. a panel of monoclonal antibodies prepared against hla-dr, nk, il-2r, t11, t4 ... | 1989 | 2555122 |
| thyrotropic activity of the ovine corticotropin-releasing factor in the chick embryo. | an injection of several doses of ovine corticotropin-releasing factor (ocrf) was made into an allantoic blood vessel of 18-day-old chick embryos. all doses used (0.5, 1, 2, and 5 micrograms) induce a quick increase of plasma corticosterone concentrations after 15 and 30 min and 1 and 2 hr and this in a dose-dependent manner with the high doses having a prolonged effect lasting up to 2 hr. an increase in plasma levels of thyroxine (t4) and triiodothyronine (t3) was observed after 1 and 2 hr. afte ... | 1989 | 2555247 |
| a site-specific endonuclease and co-conversion of flanking exons associated with the mobile td intron of phage t4. | the product of the td intron open reading frame (orf) of phage t4 is required for high-frequency transfer of the intervening sequence from intron-plus (in+) to intron-minus (in-) alleles. in vivo studies have demonstrated that the td orf product targets cleavage of td in- dna, and that cleavage is correlated with intron inheritance [quirk et al., cell 56 (1989) 455-465]. in the present study we show by in vitro synthesis of the td intron orf product, that the protein possesses endonuclease activ ... | 1989 | 2555262 |
| identification of a 27-kda protein with the properties of type ii iodothyronine 5'-deiodinase in dibutyryl cyclic amp-stimulated glial cells. | type ii iodothyronine 5'-deiodinase (5'd-ii) catalyzes the intracellular conversion of thyroxine (t4) to 3,5,3'-triiodothyronine (t3), producing greater than 90% of the bioactive thyroid hormone in the cerebral cortex. in cultured glial cells, expression of this enzyme is camp dependent. exploiting the camp-dependent nature of this enzyme in these cells and utilizing n-bromoacetyl-l-3'- or 5'-[125i]thyroxine (brac[125i]t4) to affinity label cellular proteins, a 27-kda protein with the properties ... | 1989 | 2555364 |
| the essential 65-kilodalton dna-binding protein of herpes simplex virus stimulates the virus-encoded dna polymerase. | the 65-kilodalton dna-binding protein (65kdbp) of herpes simplex virus type 1 (hsv-1), the product of the ul42 gene, is required for dna replication both in vitro and in vivo, yet its actual function is unknown. by two independent methods, it was shown that the 65kdbp stimulates the activity of the hsv-1-encoded dna polymerase (pol). when pol, purified from hsv-1-infected cells, was separated from the 65kdbp, much of its activity was lost. however, addition of the 65kdbp, purified from infected ... | 1989 | 2555539 |
| cruciform cutting endonucleases from saccharomyces cerevisiae and phage t4 show conserved reactions with branched dnas. | we have purified a cruciform dna resolving endonuclease (endo x3) greater than 1000-fold from crude extracts of mitotically growing saccharomyces cerevisiae. the enzyme shows high specificity for dnas with secondary structures and introduces characteristic patterns of staggered 'nicks' in the immediate vicinity of the structure. the following substrates were analyzed in detail: (i) naturally occurring four-way x junctions in cruciform dna of a supercoiled plasmid; (ii) synthetic four-way x junct ... | 1989 | 2556268 |
| physical structure and molecular cloning of equine cytomegalovirus dna. | restriction endonuclease (re) mapping studies and molecular hybridization analyses were conducted to determine the molecular structure of the genome of equine cytomegalovirus (ecmv). the ecmv genome is a linear, double-stranded dna with a molecular size of 126 +/- 0.6 mda (189 kbp). a library of cloned bamhi, ecori, and hindiii fragments of the viral genome was used to construct re maps. individual 32p-labeled cloned dna fragments were hybridized to southern blots of viral genomic dna digested t ... | 1989 | 2556843 |
| superhelical stress restrained in plasmid dna during repair synthesis initiated by the uvra, b and c proteins in vitro. | purified uvra, uvrb, uvrc, uvrd, pola and lig proteins from escherichia coli have been used to assess the effect of nucleotide excision repair on the conformation of native negatively supercoiled plasmid dna in an in vitro test system. the analysis of labeled reaction products on specific gel systems suggests that the uvr excinuclease has the ability to restrain the superhelical stress in the template dna during the repair process. this feature, observed in the case of the uvr system is not foun ... | 1989 | 2557590 |
| energy expenditure associated with sodium/potassium transport and protein synthesis in skeletal muscle and isolated hepatocytes from hyperthyroid sheep. | the object of the present study was to determine the effect of thyroxine (t4) treatment of sheep on protein synthesis and associated energy costs in skeletal muscle and hepatocytes. protein synthesis, and ouabain-sensitive and cycloheximide-sensitive respiration in isolated intercostal muscle and hepatocytes were determined in sheep after 5 weeks of daily injections of either saline or t4. plasma t4 and total triiodothyronine (t3) concentrations were doubled and free t3 concentrations were quadr ... | 1989 | 2557888 |
| comparison of cooperative and isolated site binding of t4 gene 32 protein to ssdna by 1h nmr. | deuteriation of all aromatic protons of gene 32 protein (g32p) from phage t4, followed by selective introduction of specific protons, has allowed the precise identification of the number and magnitude of the chemical shift changes induced in the aromatic protons when g32p binds noncooperatively or cooperatively to nucleotides. signals from five tyr residues are shifted by binding of g32p to d(pa)8 or d(pa)40-60; however, the change from noncooperative, d(pa)8, to cooperative, d(pa)40-60, binding ... | 1989 | 2557909 |
| substrate properties of 25-nt parallel-stranded linear dna duplexes. | four 25-nt oligonucleotides consisting of sequences of da and dt (d1-4) have been synthesized. as shown in a companion paper (rippe et al., 1989), the two combinations d1.d3 and d2.d4 form normal antiparallel duplexes, whereas the pairs d1.d2 and d3.d4 constitute duplexes with the same sequences, but with the two strands parallel to each other. the activities of the following dna processing enzymes and chemical reagents on the parallel stranded (ps) and antiparallel stranded (aps) duplexes were ... | 1989 | 2558723 |
| [use of non-traditional methods of examination in the differential diagnosis of cancer and tuberculosis of the lungs]. | the immunoradiometric assay of the blood serum was performed in 61 patient with cancer and in 57 patients with tuberculosis of the lungs to determine the content of adrenocorticotropic hormone (acth), hydrocortisone, triiodothyronine++ (t3), thyroxin (t4), ferritin and carcinoembryonic antigen (cea). in 200 patients (104 suffered from pulmonary tuberculosis, 9--pulmonary cancer), entropy of the protein fractions of the blood serum was determined. it was established that in differential diagnosis ... | 1989 | 2559239 |
| [the advantages of reprimum therapy in pulmonary sarcoidosis and other granulomatous diseases]. | in sarcoidosis and other granulomatous non-caseous diseases, the election treatment is immunosuppressive, mainly with cortisones that ensure more than 70% lasting remissions. continuous use of cortisones for a long time (8-30 months) in high doses leads to serious side effects: gastric and intestinal ulcers, obesity, osteoporosis, suprarenal dysfunction, sensitivity to infections. good results and elimination of the important side effects were obtained by treatment with reprimum--a semisynthetic ... | 1989 | 2559463 |
| creatine kinase-bb activity in malignant tumors and in sera from patients with malignant diseases. | creatine kinase (ck ec 2.7.3.2) and ck-bb activity was analyzed in 41 malignant tumors of 6 different sites and different histological structures. the same analyses were done on 150 sera of patients with malignant diseases of various localizations. the rate of ck activity was determined kinetically, whereas tissue and serum ck-bb were separated chromatographically (mercer). insofar as malignant tumor tissues are concerned, the highest average rate of ck-bb activity was detected in tumors of the ... | 1989 | 2559523 |
| observations on blood leucocytes and lymphocyte subsets in sheep infected with bovine leukaemia virus: a progressive study. | haematological parameters and reactivity of lymphocyte antigens to monoclonal antibodies were studied over a 10-month period in sheep experimentally infected with bovine leukaemia virus (blv). blv-inoculated animals seroconverted within 1 month and showed a significant lymphocytosis 2-6 weeks after infection. control animals inoculated with blv-free lymphocytes showed a stronger and more immediate neutrophil response than those inoculated with blv-positive lymphocytes. one month after infection, ... | 1989 | 2559536 |
| a model for the complex between the helix destabilizing protein gp32 of bacteriophage t4 and single-stranded dna. | a model for the structure of the complex between the helix-destabilizing protein of bacteriophage t4, gp32, and single-stranded dna is proposed. in this model the bases are arranged in a helix, that is characterized by a relatively large distance between successive bases, a substantial base tilt, in combination with a small rotation per base. this helix is further organized into a tertiary structure, possibly a superhelix, of which the corresponding protein shell corresponds to the relatively ri ... | 1989 | 2559746 |
| circadian rhythm parameters of endocrine functions in elderly subjects during the seventh to the ninth decade of life. | the circadian rhythm of 17 endocrine parameters (acth, aldosterone, cortisol, c-peptide, dhea-s, fsh, growth hormone, insulin, lh, 17-oh progesterone, prolactin, testosterone, total t3, total t4 and tsh and estradiol and progesterone in women only) were studied in 63 clinically apparently healthy men (124 profiles) and 86 women (154 profiles) during the 7th to 9th decade of life. the subjects lived under very uniform conditions in a home for the aged with their daily schedule standardized by ins ... | 1989 | 2560701 |
| a simple and versatile method for the preparation of vector-primers by adapter-end-primer ligation. | a group of efficient cdna cloning strategies employs vector-primers where cdna synthesis starts from the oligo(dt)-primer tail, which is conventionally attached to cloning vectors by use of terminal deoxynucleotidyl transferase. an alternative, efficient and more versatile method of vector-primer preparation is to directly ligate, by use of t4 dna ligase, a double-digested vector, e.g., ptz18r/pst i/bam hi, to a synthetic (bam hi)-adapter-end-primer, 5'-pgatcc-tn or 5'-pgatcc-site-specific seque ... | 1989 | 2561065 |
| amino acid similarities to other proteins offer insights into roles of umud and umuc in mutagenesis. | the products of the umud and umuc genes are required for most uv and chemical mutagenesis in escherichia coli. the genes are organized in an operon that is repressed by lexa and regulated as part of the sos response. the umud protein shares homology with the carboxyl-terminal domain of lexa. genetic evidence now indicates that reca-mediated cleavage activates umud for its role in mutagenesis. the cooh-terminal fragment of umud is both necessary and sufficient for this role. similarities of umud ... | 1989 | 2561111 |
| the effect of thyroid hormones on the membrane adenylate cyclase activity in sickle red blood cells in vitro. | a comparative study of thyroid hormone responsive membrane adenylate cyclase activity has been carried out on normal (hb-aa) and sickle cell (hb-ss) subjects. thyroid hormones (t3 and t4) and selected analogues (triac and dlt) enhanced adenylate cyclase activity in hb-aa membranes. the interaction of hb-ss membranes with the hormones and analogues was not significant except for t3 which moderately stimulated hb-ss membrane adenylate cyclase. we suggest that circulating irreversibly sickled cells ... | 1989 | 2561120 |
| myod and achaete-scute: 4-5 amino acids distinguishes myogenesis from neurogenesis. | the myogenic determination gene myod contains a 60 amino acid domain that is necessary for both sequence-specific dna binding and myogenic conversion of transfected c3h10t1/2 mouse embryo fibroblasts. we have generated deletion, insertion, and substitution mutants to probe the structure and function of this region, both in vitro and in vivo. our results are consistent with a previous proposal that a helix-loop-helix (hlh) motif mediates protein dimerization. a highly basic region located immedia ... | 1989 | 2562185 |
| major depressive disorder and immunity. role of age, sex, severity, and hospitalization. | an association between depression and altered immunity has been suggested but has not been consistently demonstrated. we have studied 91 patients with unipolar major depressive disorder, and no mean differences were found between the patients and concurrently studied matched controls in mitogen-induced lymphocyte proliferation, lymphocyte subsets, and natural killer cell activity. there were, however, significant age-related differences between the depressed patients and controls in mitogen resp ... | 1989 | 2562915 |
| immunopathogenic mechanisms of hiv infection. | the acquired immunodeficiency syndrome (aids) follows infection with the human immunodeficiency virus (hiv) after a long and variable period of time. although hiv can be rapidly cytopathic in vitro for t4 cells, during early in vivo infection, t4 cell numbers are normal and few infected t4 cells can be found. there is increasing evidence that cells of the monocyte/macrophage lineage, including bone marrow precursor cells, can be infected by hiv and are relatively resistant to the cytopathic effe ... | 1989 | 2562941 |
| glucoregulatory function of thyroid hormones: role of pancreatic hormones. | glucose metabolism was investigated in humans before and 14 days after 300 micrograms l-thyroxine (t4)/day using a sequential clamp protocol during short-term somatostatin infusion (500 micrograms/h, 0-6 h) at euglycemia (0-2.5 h), at 165 mg/dl (2.5-6 h), and during insulin infusion (1.0 mu.kg-1.min-1, 4.5-6 h). t4 treatment increased plasma t4 (+96%) and 3,5,3'-triiodothyronine (t3, +50%), energy expenditure (+8%), glucose turnover (+32%), and glucose oxidation (glucox +87%) but decreased thyro ... | 1989 | 2563199 |
| 1 alpha,25-dihydroxyvitamin d3 receptor distribution and effects in subpopulations of normal human t lymphocytes. | receptors for 1 alpha,25-dihydroxyvitamin d3 [1,25-(oh)2d3] are expressed upon activation of human lymphocytes, and the hormone inhibits in vitro the proliferation of mitogen-activated lymphocytes. in this study we examined the distribution of the 1,25-(oh)2d3 receptor protein in the two major subsets of t lymphocytes (t helper and t suppressor cells) and the effect of the hormone on their respective rates of proliferation. we activated normal lymphocytes in the presence of monocytes with phytoh ... | 1989 | 2564005 |
| train-of-four fade during onset and recovery of neuromuscular block: a study in non-anaesthetized subjects. | the actions of alcuronium, vecuronium and tubocurarine have been studied in the isolated forearms of six healthy, non-anaesthetized volunteers. the responses of adductor pollicis were measured during onset and recovery of neuromuscular block for each agent. there was a drug-related disparity between mechanomyogram (mmg) and electromyogram (emg) measurement of the first response of the train-of-four (t1) and of the ratio of the fourth (t4) to the first response (tof ratio). there were significant ... | 1989 | 2564783 |
| t cell regulation of human b cell proliferation and differentiation. regulatory influences of cd45r+ and cd45r- t4 cell subsets. | the immunoregulatory functions of human t4 cell subpopulations defined by mab to the cd45r molecule (2h4) were examined. both cd45r- and cd45r+ t4 cells that had been treated with mitomycin c (cd45r- and cd45r+ t4-mito) provided help for the generation of ig-secreting cells (isc) in cultures stimulated by pwm or by immobilized mab to cd3 (64.1). il-2 enhanced the generation of isc in pwm-stimulated cultures and in anti-cd3-stimulated cultures containing cd45r+ t4-mito. the generation of isc was ... | 1989 | 2564858 |
| cardiac responses to the microinjections of excitatory amino acids into the intermediolateral cell column of the rat spinal cord. | sympathoexcitatory cardiovascular responses to the microinjections of l-glutamate into the intermediolateral cell column (iml) of the upper thoracic cord (c8 to t4) were studied. mean arterial pressure (map), heart rate (hr), the rate of increase in the left ventricular pressure (dp/dt) and contractility index were monitored in immobilized and artificially ventilated male wistar rats anesthetized with pentobarbital or isoflurane. on the right side, microinjections (10-20 nl) of l-glutamate (0.9- ... | 1989 | 2565136 |
| the effect of yohimbine on plasma levels of t3, t4 and cortisol in xylazine-immobilized white-tailed deer. | 1. the effect of yohimbine (y) on blood levels of thyroxine (t4), triiodothyronine (t3), and cortisol was investigated in 5 mature male white-tailed deer immobilized with xylazine hydrochloride (x). 2. t4 levels were erratic in x-treated deer, but stabilized in the x- and y-treated deer. 3. t3 remained unchanged in both groups. 4. cortisol levels have increased in x-treated deer, but declined in x- and y-treated deer. 5. yohimbine is a potent and safe antidote of x not affecting t3 and t4. cauti ... | 1989 | 2565187 |
| [kaposi sarcoma. t4 lymphocytes secrete growth factor]. | 1989 | 2565282 | |
| progression to aids in patients with lymphadenopathy or aids-related complex: reappraisal of risk and predictive factors. | in 1985, we reported that acquired immunodeficiency syndrome (aids) developed in 14 of 81 (17%) men with generalized lymphadenopathy followed prospectively for an average of 13 months. the presence of oral thrush or constitutional symptoms, or both, or severely impaired t4+ cell responses to specific antigen (interferon-gamma production) accurately identified patients at immediate risk for aids. the purpose of the current report is to describe the progress of these 81 patients during the three a ... | 1989 | 2565690 |
| immunosuppression in men with bowenoid papulosis. | the immune status of four men with bowenoid papulosis was evaluated. each case had been refractory to multiple methods of treatment. three of the men had other infections and demonstrated a depletion of t4-helper cells. two of these patients were anergic on skin testing, and the third showed weak reactivity. the fourth patient, who had no evidence of additional infections, had a normal t4 value and t4/t8 ratio, but was anergic on skin testing. all the men were serologically negative for human im ... | 1989 | 2565705 |
| lymphocyte subpopulation with low membrane potential in the blood of cyclosporin- and prednisone-treated patients: in vivo selectivity for t4 subset. | previous work on the mode of action of csa revealed that this drug shifts the membrane potential of human periferal blood lymphocytes in vitro. recently we have analyzed lymphocytes of cyclosporin-treated transplant patients. membrane potential analysis with the dioc6(3) fluorescence dye indicates that all the studied patients have a subpopulation of lymphocytes with low membrane potential and that this population is made up predominently of okt4+ cells. however there is no correlation between t ... | 1989 | 2565726 |
| prognostic factors and staging classification of patients with epidemic kaposi's sarcoma. | two hundred twelve patients with acquired immune deficiency syndrome (aids)-related kaposi's sarcoma (ks) were followed prospectively. univariate and multivariate analyses were performed to determine significant predictors of survival and development of opportunistic infection (oi) from the time of diagnosis of ks. clinical variables analyzed were age at onset, presence of systemic symptoms, prior or coexistent oi, development of oi greater than 3 months following ks diagnosis, and extent of dis ... | 1989 | 2565954 |
| specific interaction of aurintricarboxylic acid with the human immunodeficiency virus/cd4 cell receptor. | the triphenylmethane derivative aurintricarboxylic acid (ata), but not aurin, selectively prevented the binding of okt4a/leu-3a monoclonal antibody (mab) and, to a lesser extent, okt4 mab to the cd4 cell receptor for human immunodeficiency virus type 1 (hiv-1). the effect was seen within 1 min at an ata concentration of 10 microm in various t4+ cells (mt-4, u-937, peripheral blood lymphocytes, and monocytes). it was dose-dependent and reversible. ata prevented the attachment of radiolabeled hiv- ... | 1989 | 2566170 |
| graves' hyperthyroidism following primary hypothyroidism: sequential changes in various activities of thyrotropin receptor antibodies. | a 40-year-old male who developed graves' hyperthyroidism following primary hypothyroidism is reported. he presented with clinical signs of hypothyroidism and concomitant myasthenia gravis. the thyroid was not palpable. he was treated with t4, pyridostigmine and prednisolone. one year later he developed hyperthyroidism and goitre. his initial serum igg had no intrinsic thyroid stimulating activity, but showed almost complete inhibition of tsh-stimulated camp generation (99.4%, normal less than 38 ... | 1989 | 2566247 |
| t4+ cell production of interferon gamma and the clinical spectrum of patients at risk for and with acquired immunodeficiency syndrome. | 1989 | 2566301 | |
| expression of hiv antigens at the surface of infected t4 cells: immunoelectron microscopic evidence of an immunogenic phase prior to the viral release. | hiv antigens were detected by immunoelectron microscopy at the surface of human and simian t4 lymphocytes that had been infected in vitro. hiv antigens were detected at the surface of cells exhibiting viral particles but also at the surface of cells before the release of virions. the latter cells may be considered immunogenic since they are capable of triggering specific immune responses without the cytopathic effects due to viral release. | 1989 | 2566320 |
| seasonal levels of cortisol, triiodothyronine and thyroxine in male axis deer. | 1. seasonal plasma levels of thyroxine (t4), triiodothyronine (t3) and cortisol were investigated between november and june in seven penned male axis deer. 2. no distinct seasonal variation of cortisol has been detected. the levels oscillated between 1 and 5 micrograms/dl. 3. the stress of immobilization and sampling had little effect on cortisol levels. concentrations remained mostly stable in three consecutive samples taken 10 min apart. 4. t3 concentrations were stable between november and ma ... | 1989 | 2566417 |
| neopterin: a predictive marker of acquired immune deficiency syndrome in human immunodeficiency virus infection. | in 79 homosexual men infected with human immunodeficiency virus (hiv), urinary neopterin was significantly higher as compared with 70 hiv-seronegative men in the same cohort (p less than 0.0001). this highly significant association was found both for naturally occurring oxidized (native) neopterin and for total neopterin (native plus chemically oxidized forms). in prospective follow-up for 18 months, the odds that the acquired immune deficiency syndrome (aids) would develop were elevated 25-fold ... | 1989 | 2566673 |
| hsv hepatitis in the mouse: a light and electron microscopic study with immunohistology and in situ hybridization. | in order to characterize better the morphology and immune response in acute necrotizing hsv infection, murine hsv hepatitis was examined. balb/c mice were inoculated intraperitoneally with 10(6) plaque-forming units (pfu) of hsv-1 (lenette) and hsv-2 (d316). in both groups half the animals were pretreated with silica particles to block macrophage function. up to 6 days after infection four mice from each group were sacrificed at daily intervals and the livers were examined by light and electron ... | 1989 | 2567083 |
| predictive markers for the acquired immunodeficiency syndrome (aids) in hemophiliacs: persistence of p24 antigen and low t4 cell count. | to investigate the predictive value of assays for human immunodeficiency virus (hiv) p24 antigen, p24 antibody, and gp120 antibody compared with t4 cell counts. | 1989 | 2567142 |
| effective method for prediction of transient hypothyroidism in neonates born to mothers with chronic thyroiditis. | an effective method of prediction of neonatal transient hypothyroidism was examined in 105 neonates (including a pair of twins) born to mothers with chronic thyroiditis (92 mothers with goitrous hashimoto's disease and 12 with primary atrophic hypothyroidism). antithyroid microsomal antibody was measured by a hemagglutination technique (mcha), and antithyroid-stimulating hormone (tsh) receptor antibody by both radioreceptor assay (tbii) and biologic thyroid-stimulation blocking assay (tsbab). fo ... | 1989 | 2567172 |
| in vivo depletion of bot4 (cd4) and of non-t4/t8 lymphocyte subsets in cattle with monoclonal antibodies. | the effect on certain immune responses of depleting two distinct lymphocyte subpopulations in vivo by inoculating calves with monoclonal antibodies (mab) was examined. an mab directed against the bot4 antigen (the bovine homologue of cd4) effectively removed the bot4+ lymphocytes from peripheral blood mononuclear cells (pbm). compared to controls, treated calves showed a reduced antibody response to human o red blood cells and to ovalbumin. pbm prepared from bot4-depleted animals also had a sign ... | 1989 | 2567247 |
| regulatory t-cell function in primary humoral immunodeficiency states. | the function of b cells and highly purified t-cell subpopulations was studied in patients with various primary humoral immunodeficiencies. in the presence of normal t4+ cells b cells of the patients with x-linked agammaglobulinemia (xla) were not capable either for igm or igg synthesis, whereas b cells of the patient with hyper-igm syndrome and three of eight patients with common variable immunodeficiency (cvi) could produce normal amounts of igm, but not igg. all patients with xla and the patie ... | 1989 | 2568491 |
| advanced immunodeficiency as a risk factor for heterosexual transmission of hiv. | to define the rate of hiv infection and associated risk factors among heterosexual partners of hiv-infected individuals, 80 partners and 77 index cases were examined for clinical, behavioural and virological variables. the study population included 61 male and 16 female index cases, of whom 40 were european and 37 african. multiple sexual partners in central africa was the risk factor for hiv infection in 83%. the overall infection rate among the contact cases was 45%, 53% among female partners ... | 1989 | 2568844 |
| okt4/okt8 ratio in asymptomatic heroin addicts. | the okt4 (helper) and okt8 (suppressor) lymphocytic subpopulations were enumerated in a sample of 60 asymptomatic drug addicts and in 17 controls. no significant differences in the ratio could be found that could not be explained by the action of hiv. it can be concluded that heroin itself was not responsible for any alteration in the t4/t8 ratio in the population considered. | 1989 | 2569333 |
| atracurium and vecuronium interaction with gentamicin and tobramycin. | drug interactions between the aminoglycosides (tobramycin and gentamicin) and atracurium and vecuronium were studied prospectively in 44 patients. twenty-two patients had therapeutic serum levels of tobramycin or gentamicin and 22 served as controls. onset time, clinical duration, and time to spontaneous recovery of t4/t1 ratio of 0.70 after atracurium or vecuronium injection were measured. no statistically significant differences were found in onset time, but clinical duration and time to recov ... | 1989 | 2569365 |
| levels of t4, t3 and cortisol in the blood serum of the european bison (bison bonasus) in the winter period. | 1. in the blood of 56 european bison, the levels of t4, t3, and the ratio of t3:t4 and cortisol were studied. 2. between december and march, the t4 level changed from about 53 to 83 ng/ml of blood serum but in april it increased to 90 ng/ml. 3. the level of t3 in this period was 0.817-1.475 ng/ml and in april it was 2.40-3.40 ng/ml. 4. the ratio of t3:t4 was 0.015-0.024 and in april it increased to 0.027-0.052. 5. the level of cortisol changed from 1.00 to 6.70 ng/ml and in april it was from 1.0 ... | 1989 | 2569374 |
| spread of analgesia and ventilatory response to carbon dioxide following epidural somatostatin. | the effects of somatostatin, injected into the epidural space, on analgesia and control of ventilation were studied in 25 patients aged 41 +/- 9 yrs (mean +/- sd). the patients were allocated to three groups to receive: group i--1 mg of somatostatin in 2 ml saline (n = 13); group ii--1 mg of somatostatin in 10 ml saline (n = 6); and group iii--somatostatin in a loading dose of 250 micrograms followed by an infusion of 125 micrograms h-1 (n = 6). segmental cutaneous analgesia, assessed by pinpric ... | 1989 | 2569394 |
| studies on lymphocyte subpopulations and nk cell activities in epileptic patients. | the subpopulations of t lymphocytes, b lymphocytes and macrophages of the peripheral blood in 58 cases of epilepsia were studied with mcab by ifa method. the activity of natural killer (nk) cells of peripheric blood monocyte was investigated by 51cr-releasing method. 22 healthy donors of about the same age served as controls. the results showed that the percentage of t3 and t4 lymphocytes in epileptic patients was decreased, and the ratio of t4/t8 was markedly reduced as compared to control grou ... | 1989 | 2569541 |
| preparation of misacylated aminoacyl-trna(phe)'s useful as probes of the ribosomal acceptor site. | several pyroglutamylaminoacyl-trna's were prepared by t4 rna ligase mediated condensation of synthetic pyroglutamylaminoacyl-pcpa's with trna's from which the last two nucleotides at the 3'-end had been removed. the derived pyroglutamylaminoacyl-trna's were incubated in the presence of calf liver pyroglutamate aminopeptidase, which effected their conversion to free aminoacyl-trna's. the lack of contaminating esterase activities in the pyroglutamate aminopeptidase was verified by direct assay for ... | 1989 | 2569893 |
| a role for cd4+ but not cd8+ t cells in immunity to schistosoma mansoni induced by 20 krad-irradiated and ro 11-3128-terminated infections. | the role of cd4+ (l3/t4+) and cd8+ (lyt-2+) t cells in immunity to schistosoma mansoni induced by 20 krad-irradiated and ro 11-terminated infections in mice was investigated directly by in vivo depletion of these subsets with cytotoxic rat monoclonal antibodies (mab). effective physical depletion was demonstrated by flow cytometric analysis and immunohistochemical staining. functional depletion of helper activity following anti-cd4 treatment was indicated by an abrogation of concanavalin a(con a ... | 1989 | 2570035 |
| mass and in situ molar activity of tyrosine hydroxylase in the median eminence. effect of thyroidectomy and thyroid hormone replacement. | the effects of thyroidectomy and thyroid hormone replacement on the mass and in situ molar activity of tyrosine hydroxylase (th) in the median eminence (me) and superior cervical ganglia (scg) of male rats were investigated. the tissue specificity of these effects were evaluated by comparing the me with the superior cervical ganglion (scg). all animals were thyroparathyroidectomized (tx) or sham tx. tx rats were treated daily for 3 weeks with 0.15 m nacl (solvent vehicle) or l-thyroxine (t4). tw ... | 1989 | 2570369 |
| detection of hiv-1 dna in different subsets of human peripheral blood mononuclear cells using the polymerase chain reaction. rapid communication. | the presence of hiv-1 proviral dna was determined in cd4 cells, cd8 cells and macrophages/monocytes obtained from peripheral blood of 8 hiv-1 infected persons. using the polymerase chain reaction (pcr) we were able to detect proviral dna in the extracts of only 10(2)-10(3) cd4 (t4) cells. in contrast, 10(5) cd8 cells did not contain detectable amounts of proviral dna. surprisingly, in four of our eight patients studied no hiv-1 dna was found in macrophages. in peripheral blood, every hundred t4 ... | 1989 | 2570562 |
| glucoregulatory function of thyroid hormones: evidence for an insulin-independent effect. | the effect of hyperglycemia on glucose kinetics was investigated in normal and t4-treated miniature pigs. 1. t4-treatment increased basal plasma glucose (g) (+ 20%) and glucose turnover (+36%). 2. glucose infusion (2 mg/kg x min) in controls increased insulin and glucose utilization (rd), but decreased glucagon and hepatic glucose production (ra). after t4-treatment glucose increased insulin, decreased glucagon and ra but only a slight effect on g and rd were observed. 3. infusing glucose + soma ... | 1989 | 2570710 |
| effect of rate of injection on the neuromuscular block produced by vecuronium. | a subparalytic dose (0.015 mg/kg) of vecuronium bromide was administered to matched pairs of patients undergoing routine dental surgery under enflurane-nitrous oxide/oxygen anesthesia either as a rapid bolus injection or as a slow infusion over 5 min. it was demonstrated that bolus injection produced peak plasma levels of drug considerably greater than those following slow infusion. time to maximum block was more rapid following bolus (368 +/- 84 [sd] sec) than by infusion (615 +/- 88 sec), but ... | 1989 | 2572184 |
| [study of ige and t lymphocyte subsets in patients with serious bronchial asthma]. | in the present study, 30 patients with serious asthma were selected for the investigation of ige and t lymphocyte subsets in pbl. by the indirect mcab immuno-spa assay, it was observed that the percentages of okt3 and okt8 but the percentage of okt4 subset and t4/t8 ratio were significantly higher than those of normal persons (p less than 0.01). the total ige in serum of patients (performed by elisa) was also much higher than that of normal persons (p less than 0.05). in addition, there was a cl ... | 1989 | 2572332 |
| response to pneumococcal vaccine among asymptomatic heterosexual partners of persons with aids and intravenous drug users infected with human immunodeficiency virus. | antibody responses to pneumococcal vaccine were studied in asymptomatic heterosexual partners of persons with aids and intravenous drug users seropositive for human immunodeficiency virus (hiv). serum antibodies to 12 pneumococcal capsular antigens were measured by radioimmunoassay. eleven intravenous drug users seropositive for hiv, 13 seronegative intravenous drug users, and 10 each seropositive and seronegative sexual partners received 23-valent pneumococcal vaccine. additional unvaccinated m ... | 1989 | 2572650 |
| in vitro accumulation of thyroid hormones by cultured rat hepatocytes and the biliary excretion of iodothyronines in rats treated with a novel histamine h2-receptor antagonist. | the administration of sk&f 93479, a novel histamine h2 antagonist, to wistar rats has been shown to produce thyroid lesions associated with an increased clearance of plasma thyroxine (t4) and elevated plasma tsh concentrations. to determine if these changes were mediated via an increase in the hepatic clearance of thyroid hormones, the biliary clearance and hepatic accumulation of iodothyronines were measured in rats and cultured hepatocytes treated with sk&f 93479. both the in vitro and in vivo ... | 1989 | 2573172 |
| intracellular ca2+ and cytotoxicity. | following injury or activation in some immune cell lines, elevation of intracellular ca2+ concentration (cai2+) is an early and major event that precedes cell death. agents shown to elevate cai2+ and to result subsequently in the death of some cells include human immunodeficiency virus (hiv) (in t4+ cells), 25-hydroxy cholesterol, tumor necrosis factor (tnf), cyclosporine, dexamethasone, alpha-interferon, and ca2+ ionophores. the effects of these agents, both on cai2+ and on cytotoxicity, are ad ... | 1989 | 2573316 |
| the natural history of human immunodeficiency virus infection in a haemophilic cohort. | 112 haemophilic patients infected with hiv were followed up with clinical and laboratory assessment between 1 december 1979 and 30 november 1988. sixty-six (59%) of the patients developed hiv-related clinical symptoms and 22 (20%) developed aids. twenty (18%) of the patients developed p24 antigenaemia. amongst the 59 patients whose date of seroconversion could be estimated the calculated 8-year cumulative incidence of aids was 40% (symptoms 73%). for the whole cohort of 112 patients, the median ... | 1989 | 2573389 |
| analysis of c-erbb-2 expression in breast carcinomas with clinical follow-up. | various monoclonal antibodies reactive with protooncogene products or tumor-associated antigens have been utilized to investigate breast carcinoma biology or antigen expression with potential prognostic relevance. murine monoclonal antibody ta1, generated by immunization of balb/c mice with whole c-erbb-2 (neu) transformed nih/3t3 cells, recognizes the extracellular domain of the c-erbb-2 protein and binds a mr 185,000 protein by immunoprecipitation. using avidin-biotin-peroxidase techniques and ... | 1989 | 2573426 |
| precursor frequency of human t4 cells responding to stimulation through the cd3 molecular complex: role of various cytokines in promoting growth and il2 production. | the frequency of human t4 cells induced to grow and produce il2 in response to the anti-cd3 mab, 64.1, was examined. t4 cells were cultured at limiting dilution and stimulated with either soluble or immobilized 64.1 in the presence of various cytokines and/or irradiated b lymphoblastoid cells as accessory cells (ac). the frequency of responding cells was assessed by examining wells microscopically for visible growth and supernatants for il2. immobilized, but not soluble, 64.1 was able to induce ... | 1989 | 2573432 |
| comparison between constant-protein, calorie-restricted and protein-restricted, calorie-restricted diets on growth, in vitro lipogenesis and plasma growth hormone, thyroxine, triiodothyronine and somatomedin-c (sm-c) of young chickens. | 1. we studied the effects of calorie-restricted, constant-protein and calorie-restricted, protein-restricted diets on growth and in vitro metabolism of male chickens from select (cobb line 500) and byproduct (cobb female line) lines of broiler chickens. 2. chickens consumed 40, 60, 80 or 100% of a prescribed formula for dietary energy (body weight in g0.70 x 16.7 kj) in the presence of set (cep) or varied dietary protein (vep). 3. chickens fed vep were heavier (p less than 0.05) at all energy in ... | 1989 | 2573452 |
| hiv heterosexual transmission in hemophilia couples: lack of relation to t4 number, clinical diagnosis, or duration of hiv exposure. | six (13%) of 45 female partners of 45 hiv antibody positive [ab(+)] hemophiliacs in western pennsylvania have become hiv ab(+), a mean of 10.4 months from the time of first exposure. by comparison, the mean time from first exposure to the last/recent exposure in the hiv ab(-) female partners was 54.2 months. all six hemophiliacs who transmitted hiv to their female partners were asymptomatic, with mean t4 of 403 +/- 51/mm3 at the time of estimated seroconversion in the female; currently, their me ... | 1989 | 2573720 |
| phase i study of low-dose zidovudine and acyclovir in asymptomatic human immunodeficiency virus seropositive individuals. | the combination of zidovudine and acyclovir has shown in vitro antiretroviral activity and led to short-term improvement in patients with symptomatic human immunodeficiency disease (hiv) disease. we performed a phase i study of zidovudine (500 mg/day) plus acyclovir (2 or 4 g/day) in asymptomatic hiv-seropositive men to investigate pharmacokinetics, safety, tolerance, and immunologic effects of the combination. | 1989 | 2574006 |
| effect of fenoldopam, a dopamine d-1 receptor agonist, on pituitary, gonadal and thyroid hormone secretion. | the influence of fenoldopam, a dopamine (da) d-1 receptor agonist, on basal and gnrh/trh stimulated prl, gh, lh, tsh, testosterone and thyroid hormone secretion was studied in nine normal men. all men received 4-h infusions of either 0.9% saline or fenoldopam at an infusion rate of 0.5 microgram/kg min, 12-16 ml/h, adjusted according to weight. after 3 h of infusion, 50 micrograms gnrh and 100 micrograms trh was given i.v. blood samples were collected every 15 min from 1 h before to 1 h after th ... | 1989 | 2574081 |
| thyroid function in sex-linked and autosomal dwarf chickens. | thyrotropin-releasing hormone (trh) and thyrotropin (tsh) elevated plasma thyroxine (t4) and triiodothyronine (t3) concentrations in a (euthyroid) control line of chickens and in an autosomal dwarf strain. these agents were ineffective in sex-linked dwarf (sld) chickens. similarly, while somatostatin (srif) lowered plasma t4 and t3 concentrations in autosomal dwarf (adw) chickens and controls, it had no inhibitory effect in the sld strain. these results suggest that an impairment in the hypothal ... | 1989 | 2574126 |
| isolation of hiv using t-lymphocyte 'panning': methodological aspects. | a technique for isolation of hiv using selective cultures of t4 cells obtained from peripheral blood by immunochemical separation was developed and optimized. using this method infectious virus could be isolated in single isolation attempts from 89% of 35 hiv-infected patients in different stages of immunodeficiency. this isolation frequency was virtually independent of the stage of the disease, in contrast to the results obtained by the conventional isolation technique based on peripheral monon ... | 1989 | 2574183 |
| evaluation of tsh receptor antibody by 'natural in vivo human assay' in neonates born to mothers with graves' disease. | neonatal thyrotoxicosis induced by transferred tsh receptor antibody (trab) is the ideal human in-vivo experimental system for the evaluation of trab. the clinical significance of circulating trab in graves' disease was evaluated by this 'natural in-vivo human assay'. trab activity in vitro was measured by radioreceptor assay (thyrotrophin-binding inhibitor immunoglobulin, tbii) and sensitive camp accumulation assay using frtl-5 cells (thyroid-stimulating antibody, tsab). further, the binding-st ... | 1989 | 2575017 |
| the long-term outcome of thyroidectomy for sporadic non-toxic goitre. | to study the long-term outcome after thyroidectomy, 113 sporadic non-toxic goitre patients who underwent thyroidectomy in our hospital in the period 1974-1983, were studied. five patients complained of recurrent goitre; a goitre was found on inspection and palpation in these five and in 15 others. there were no differences between the 20 patients with goitre and the 93 patients without goitre with regard to sex, age, duration of goitre, indication and type of thyroidectomy, postoperative thyroid ... | 1989 | 2575018 |
| high levels of oral yeasts in early hiv-1 infection. | ten human immunodeficiency virus-1 (hiv-1) infected homosexual or bisexual individuals (ages 24-45) with no history of opportunistic infection were examined, by culture, for the presence of yeasts in whole saliva and on oral mucosa. all were hiv-1 antibody-positive men, non-smokers, non-denture wearers, and taking no medication. the mean salivary level of yeast was four logs higher in the hiv-1 infected group compared to a control group of normal, unmedicated, non-smoking men (ages 20-41) who de ... | 1989 | 2575167 |
| highly specific inhibition of human immunodeficiency virus type 1 by a novel 6-substituted acyclouridine derivative. | a novel 6-substituted acyclouridine derivative, 1-[(2-hydroxy-ethoxy) methyl]-6-phenylthiothymine (hept), has proved to be a potent and selective inhibitor of human immunodeficiency virus type 1 (hiv-1) in vitro. hept inhibits hiv-1 replication in various t4 cell cultures as well as peripheral blood lymphocytes and macrophages. the 50% antiviral effective concentration for hiv-1 (htlv-iiib) in mt-4 cells is 7.0 microm, while the 50% cytotoxic concentration for mock-infected mt-4 cells is 740 mic ... | 1989 | 2575380 |
| endocrine effects of combined somatostatin analog and bromocriptine therapy in women with advanced breast cancer. | in this pilot clinical trial conducted in 10 postmenopausal women with advanced breast cancer, we evaluated the endocrine effects and toxicity of combined somatostatin analog and dopaminergic therapy in the attempt to suppress both growth hormone (gh) and prolactin (prl) secretion. the patients' mean age was 63 years (range: 54-77) and the average number of previous treatments was 4.8 +/- 2 (sd). all patients were treated with the somatostatin analog sms 201-995 (100-200 micrograms s.c. in a.m. ... | 1989 | 2575406 |