Publications

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toxocara vitulorum: maternal transfer of antibodies from buffalo cows (bubalis bubalis) to calves and levels of infection with t vitulorum in the calves.the levels of antibody to the excretory/secretory antigens of the infective larvae and adults of toxocara vitulorum were measured by gel precipitation and elisas in the serum and colostrum of 12 buffalo cows naturally infected with t vitulorum and in the serum of their calves. the antibody levels were compared with the extent of t vitulorum infection as judged by faecal egg counts in the calves. the patterns of bands of the larval antigens and gel precipitating antibodies in cow serum taken one ...19947973098
the disposition of lead in blood and tissues of buffalo calves (bubalus bubalis). 19947975194
comparative pharmacokinetics of fenbendazole in buffalo and cattle.swamp buffalo (bubalus bubalis) and droughtmaster cattle (bos indicus x b. taurus), fitted with gastrointestinal cannulae, were dosed intraruminally with fenbendazole at 7.5 mg/kg liveweight, together with a chromium oxide capsule and a pulse dose of nacoedta, to estimate the flow dynamics of the digesta in the rumen and duodenum. the concentrations of fenbendazole (fbz) metabolites were measured in plasma and duodenal fluid collected over 120 h. in plasma, significantly lower peak concentration ...19947985383
some enzymes of gluconeogenesis in various fractions of sarcocysts of sarcocystis fusiformis of buffalo (bubalus bubalis).a biochemical study was conducted to assess the relative presence of some enzymes of gluconeogenesis in various fractions--the cyst wall, cyst fluid and zoites--of sarcocysts of sarcocystis fusiformis obtained from the oesophageal muscles of naturally infected indian water buffalo (bubalus bubalis). the activities of fructose 1,6-diphosphatase and malic enzyme were beyond detectable limits. phosphoenol pyruvate carboxykinase (pepck) activity was maximally present in the zoites, whereas glucose 6 ...19948030180
genetic studies of water buffalo blood markers. ii. carbonic anhydrase, esterase d, malate dehydrogenase, and malic enzyme. 19948031293
standard karyotype of the river buffalo (bubalus bubalis l., 2n = 50). report of the committee for the standardization of banded karyotypes of the river buffalo.during the 10th european colloquium on cytogenetics of domestic animals held in utrech, the netherlands, in 1992, an international committee on river buffalo chromosomes was established. at the recent 8th north american colloquium on domestic animal cytogenetics and gene mapping in guelph, canada (1993), q-, g- and r-banded river buffalo karyotypes were presented and discussed. this material was used to establish the first standard karyotype of the river buffalo.19948039419
the pathology of experimental trypanosoma evansi infection in the indonesian buffalo (bubalus bubalis).six indonesian buffaloes (bubalus bubalis) were inoculated intravenously with 10(5) trypanosoma evansi, examined clinically, haematologically and serologically, and then killed 1, 2, 3, 4, 8 or 12 weeks after infection for detailed pathological study. relapsing fever was related to the waves of parasitaemia and fluctuations of pulse and respiration rates. anaemic mucous membranes, depression, weakness, refusal to walk, loss of appetite and emaciation were seen. body weight, packed cell volume, t ...19948040389
strategies for purification of four reproductive hormones from the same batch of buffalo (bubalus bubalis) pituitaries.the occurrence of different reproductive hormones like lh, fsh, tsh and prolactin, in different side fractions obtained during the extraction of buffalo pituitary glands either by the procedure of papkoff et al. [arch biochem biophys, 111 (1965) 431] or by that of ellis [endocrinology, 69 (1961) 554], was examined with the aid of antisera to respective heterologous hormones as well as bio-assays. thus in the procedure of papkoff et al., the sp-sephadex fractions could be taken for purification o ...19948045616
localization of uridine monophosphate synthase (umps) gene to river buffalo chromosomes by fish.uridine monophosphate synthase plays an important role in pyrimidine synthesis, converting orotic acid to uridine 5' monophosphate. in cattle, umps deficiency is inherited as a monogenic autosomal recessive trait. while heterozygous carriers are phenotypically normal, homozygotes are lethal in utero (shanks et al. 1992). umps has been mapped to human chromosome 3q13 (qumsiyeh et al. 1989), sheep chromosome 1q (burkin et al. 1993) and cattle chromosome 1q31 (ryan et al. 1994). in the present stud ...19948069469
trisomy-x in a sterile river buffalo. 19948197685
some blood parameters of water buffalo in different physiological conditions.the authors have studied the changes of got, gpt, alp, cpk and se in buffaloes under different physiological conditions (age, diet, different pre and post partum times). 99 adult buffaloes were classified in 8 groups on the basis of different pre, post partum time intervals. moreover, other 39 animals were divided into 4 groups: a = 60-90 day old animals, fed with reconstituted milk supplemented with vit e; b = same age, fed with maternal milk; c = 6-8 month old animals; d = 12-18 month old anim ...19938198807
the disposition of carbenicillin in blood, other body fluids and tissues of buffalo calves (bubalus bubalis). 19938212521
[morphostructural study of the lingual papillae in the buffalo (bubalus bubalus)].the morphostructural characteristics of lingual papillae in 30 adult buffaloes were studied by sem. in this species, dorsal lingual surface papillae shows different shape so that their classification is complicated. topography and morphology of conical and vallate papillae presents significant aspects. particularly, the conical papillae have a different morphological organization when they are localized over the side of the tongue apex or over dorso-lateral tract of the tongue body. the function ...19938238954
genetic studies of water buffalo blood markers. i. red cell acid phosphatase, albumin, catalase, red cell alpha-esterase-3, group-specific component, and protease inhibitor.we have developed the methodologies for typing and family studies to establish the modes of inheritance of water buffalo red cell acid phosphatase (acp), protease inhibitor (pi), and group-specific component (gc) on isoelectric focusing and albumin (alb), red cell alpha-esterase-3 (est-3), and catalase (cat) on polyacrylamide gel electrophoresis. family studies showed that pi, gc, alb, and cat are coded by autosomal genes with two codominant alleles, while est-3 is autosomal with two codominant ...19938259925
ultrastructural localization of wheat germ agglutinin binding sites on the sperm surface of water buffalo (bubalus bubalis). a fracture label study.in the present study we have examined the plasma membrane surface organization employing fluorescein isothiocyanate linked wheat germ agglutinin (wga) of the cauda epididymal and ejaculated spermatozoa of water buffalo. intramembrane particle distribution pattern in the various segments of the spermatozoa has also been observed. wga-ovomucoid gold has been used to study the distribution of sialoproteins on the sperm surface. with fracture label, wga receptor sites have been identified on the fra ...19938269396
sex determination of in vitro developed buffalo (bubalus bubalis) embryos by dna amplification.this study was conducted to determine the sex of buffalo embryos produced in vitro by amplifying male specific dna sequences using the polymerase chain reaction (pcr). this method uses three different pairs of bovine y-chromosome specific primers and a pair of bovine satellite specific primers. buffalo in vitro fertilized embryos at the 4-cell to blastocyst stage were collected at days 3, 4, 6, and 8 postinsemination, and the sex of each embryo was determined using all three different y-chromoso ...19938286109
[the possible pathways of substance transport in the muscle cysts of 2 species of sarcosporidia (sarcocystis, apicomplexa, sporozoa)].the present work is a sequel of our previous cytological investigations of the cyst-forming coccidia of the genus sarcocystis (reported elsewhere), now performed on two species from different hosts: s. muris (mouse) and sarcocystis sp. (water buffalo). acid phosphatase is synthesized in the endoplasmic reticulum of cystic zoites, its activity being seen in particular in the microenemes. as was established earlier (radchenko, 1991b), these organelles are extended regions of the smooth endoplasmic ...19938328021
chromosomal localization of the major histocompatibility complex in cattle and river buffalo by fluorescent in situ hybridization. 19938335474
chromosomal localization of omega and trophoblast interferon genes in goat and sheep by fluorescent in situ hybridization.fluorescent in situ hybridization (fish) of the cattle cdna probe btp-509 to goat (capra hircus l.) and sheep (ovis aries l.) r-banded metaphase chromosomes localized the omega (ifnw) and trophoblast (ifnt) interferon genes to goat chromosome 8q15 and sheep chromosome 2p15. caprine chromosome 8 and ovine chromosome 2p are equivalent in banding pattern to cattle chromosome 8. since fish has previously localized ifnw and ifnt genes to cattle chromosome 8q15 and river buffalo 3q15, the localization ...19938340618
purification and characterization of igg1 and igg2 from buffalo (bubalus bubalis) serum and colostrum.buffalo igg1 and igg2 were purified from serum and colostrum using salt precipitation, dialysis, gel filtration and ion-exchange chromatography. their purity was monitored by immunodiffusion and immunoelectrophoresis using anti-heavy chain specific sera and sds-page. selective binding of igg2 to protein-a was used to remove igg2 from igg1 preparations. the igg1 and igg2 had a molecular mass (mr) of 162.0 and 161.5 kd, respectively and were found to consist of heavy (h) and light (l) chains. the ...19938342260
distribution of some elements in hydatid cysts of echinococcus granulosus from buffalo (bubalus bubalis).distribution of some elements during development of endogenous daughter (hydatid) cysts of echinococcus granulosus of buffalo origin was determined by atomic absorption spectrophotometry. these analyses showed that the distribution of copper and cobalt was highest in the smallest cysts. these elements gradually decreased in the cysts as they enlarged. the distribution of zinc, iron and manganese was very high in the smallest cysts in comparison to copper and cobalt, but these three elements decr ...19938354855
electrospray mass spectrometric analysis of river buffalo (bubalus bubalis) hemoglobins. re-examination of alpha 1 and alpha 3 globin chain sequences.1. the accurate molecular weight of the globin chains from river buffalo hemoglobin components has been determined by means of electrospray mass spectrometry. 2. the es/ms analysis demonstrated that all the buffalo hb components share a common beta chain whose mass value coincides with that expected on the basis of the reported sequence. 3. the aa phenotype alpha 1 and alpha 3 globin chains exhibited a 30 da mass difference as compared to their predicted molecular weights. careful re-examination ...19938365113
spectroscopic and microscopic studies of buffalo-bull (bubalus bubalis) spermatozoa.we have examined the epididymal (caput, corpus and cauda) and ejaculated spermatozoa of bufallo-bull (bubalus bubalis) employing microscopic and spectroscopic techniques. fluorescein isothiocyanate conjugated lectins namely concanavalin a (con a), dolichos biflorus (dba), maclura pomifera (mpa), peanut agglutinin (pna), soybean agglutinin (sba) and wheat germ agglutinin (wga) were used to study the changes in the sperm surface carbohydrate make up as the spermatozoa mature. quantitative analysis ...19938384930
modification of fenitrothion-induced circulating enzymatic alterations in bubalus bubalis by 2,3-butanedione monoxime. 19938391347
effect of calmodulin-like protein from buffalo (bubalus bubalis) seminal plasma on ca2+, mg(2+)-atpase of purified plasma membrane of buffalo spermatozoa.buffalo sperm heads and tails were cleaved by sonication and isolated in relatively pure proportions i.e. 95% and 98% respectively, by discontinuous sucrose density-gradient centrifugation. purified plasma membranes from the isolated sperm heads and tails were obtained by hypotonic treatment and brief sonication followed by discontinuous sucrose density-gradient centrifugation. ca2+, mg(2+)-atpase activity was evident in plasma membrane from sperm heads and tails, although activity was greater i ...19938427418
studies on activation and levels of haemolytic complement of buffalo (bubalus bubalis). iii. c3 haemolytic activity in health and chronic disease.estimation of haemolytic complement component c3 activity in serum was done by using buffalo serum functionally depleted of c3, with methylamine. in the one-step alternate pathway (ap) haemolytic assay, c3 activity in the serum was estimated by its capacity to reconstitute the ap haemolytic activity in c3 depleted serum. levels of haemolytic c3 were determined in the sera of 70 apparently healthy buffaloes and of seven diseased buffaloes of which five were suffering from johne's disease (jd) and ...19938430503
chromosomal localization of omega and trophoblast interferon genes in cattle and river buffalo by sequential r-banding and fluorescent in situ hybridization.fluorescent in situ hybridization (fish) of the cattle cdna probe btp-509 to rba-banded cattle (bos taurus l.) chromosomes confirmed the assignment of the omega (ifnw) and trophoblast (ifnt) interferon genes to chromosome 8q15. using the same probe, these genes were also localized to river buffalo (bubalus bubalis l.) chromosome 3q15 following sequential rba-banding and fish. the extensive g- and r-banding homology observed between cattle chromosome 8 and river buffalo chromosome arm 3q supports ...19938440140
sarcocystis fusiformis: some protein metabolic enzymes in various fractions of sarcocysts of buffalo (bubalus bubalis).an investigation on the relative presence of some protein metabolic enzymes, namely aspartate aminotransferase (ast), alanine aminotransferase (alt), nad+ and nadp+ dependent glutamate dehydrogenase (gldh) and arginase in cyst wall (cw), cyst fluid (cf) and zoite (zt) fractions of the sarcocysts of sarcocystis fusiformis in the oesophageal muscles of indian water buffalo was carried out. both the transaminases were present in all the fractions of the cyst, although in variable amounts. there was ...19938447061
enterocin 226nwc, a bacteriocin produced by enterococcus faecalis 226, active against listeria monocytogenes.enterococcus faecalis 226, isolated from natural whey cultures utilized as starters in the manufacture of mozzarella cheese from water-buffalo milk, produces a bacteriocin designated enterocin 226nwc. the bacteriocin was isolated from culture supernatant fluids of the producer strain and was active against strains of the same species and listeria monocytogenes, but not against useful lactic acid bacteria. enterocin 226nwc is a protein with an apparent molecular weight of about 5800; it is relati ...19938486543
vitiligo in two water buffaloes: histological, histochemical, and ultrastructural investigations.vitiligo, a skin disease, characterized by the spontaneous loss of melanin, has been described in several animals as well as in humans. most of the reports of large domestic animals have dealt with clinical investigations without morphological data. in this report, the histological and ultrastructural characteristics of two cases of vitiligo in water buffaloes (bubalus bubalis) are presented. interestingly, many of the ultrastructural observations for vitiliginous buffaloes resemble those previo ...19938502622
purification and characterization of an alkaline proteinase from kidney cortex lysosomes.an alkaline proteinase was purified to apparent homogeneity from buffalo (bubalus bubalis) kidney cortex lysosomes by affinity chromatography on sti sepharose 4b and gel filtration over sephadex g-100. the molecular weight of the enzyme was 17,000 and 21,000 by gel filtration and sds/page respectively. the purified enzyme was optimally active at ph 8.5-9.0 at 50 degrees c and hydrolysed synthetic substrates of chymotrypsin but not those of elastase or trypsin. it was inhibited by serine proteina ...19938509124
identification of nucleolus organizer chromosomes in sheep (ovis aries l.) by sequential gbg/ag-nor and rbg/ag-nor techniques.seven gentile di puglia sheep (ovis aries l., 2n = 54) reared in southern italy were cytogenetically investigated to reveal which chromosomes carry nucleolus organizer regions (nors). the use of sequential gbg/ag-nor and rbg/ag-nor techniques in prometaphase preparations revealed nor-localization at the telomeres of chromosomes 1p, 2q, 3q, 6 and 25 on the basis of the standard r-banded karyotype. this procedure also permitted the characterization of g- and r-banding patterns of acrocentrics 6 an ...19937694824
detection of genome specific monomorphic loci in bos taurus and bubalus bubalis with oligodeoxyribonucleotide probe.a synthetic oligodeoxyribonucleotide probe (oat36) comprising nine repeats of 5'gaca 3' and several enzymes were used to analyse cow, (bos taurus) and buffalo (bubalus bubalis) genomes and a number of monomorphic loci were detected in both the species. different animals from the same species showed an almost 'similar' monomorphic hybridization pattern but animals from two separate species showed a different 'genome specific' pattern. the overall hybridization with any enzyme and probe combinatio ...19938103300
a new centric fusion translocation in cattle, rob(16;18).a barrosã bull (portugal) has been found to carry a new robertsonian translocation involving chromosomes 16 and 18 of standard cattle karyotype, as demonstrated by gbg- and rbg-banding techniques. c-banding patterns revealed the dicentric nature of this translocation. a comparison between normal cattle chromosome 16 and the q-arms of translocation chromosome and river buffalo chromosome 5 revealed the same g- and r-banding patterns, with only exception of a pericentromeric g-positive band which ...19938144362
chromosomal localization of the lysozyme gene cluster in river buffalo (bubalus bubalis l.).lysozyme (lyz) is an antibacterial enzyme which allows the digestion of bacteria present in tears and saliva. in the true stomach of ruminants lyz breaks open the bacteria of the foregut, which are subsequently digested by typical mammalian digestive enzymes, allowing the incorporation of nutrients from the bacteria. southern analysis with a single exon from a cow lysozyme gene revealed that there are about 10 genes in ruminants (irwin & wilson 1989), while pig and primates have a single lysozym ...19938156163
recurrence of rift valley fever in egypt.rift valley fever (rvf) has been recorded in man and in domestic animals in egypt after a 12-year absence. human infections were first noted in the aswan governorate in late may, 1993. only cases of ocular disease, an infrequent and late manifestation, were reported. of 41 cases, 35 were tested serologically and 27 (77%) had rvf virus-specific igm antibodies. an estimated 600-1500 infections occurred in the region. abortions in cattle and buffalo were seen concurrently and antibodies to rvfv wer ...19937901480
effects of bull and heparin and sperm concentrations on in vitro fertilization of buffalo (bubalus bubalis ) oocytes matured in vitro.a study was undertaken to assess the ability of spermatozoa from 6 buffalo bulls, at different levels of heparin and sperm concentrations, to achieve an acceptable level of fertilization in vitro. frozen-thawed spermatozoa, 3 dosages of heparin (0, 10 and 100 ug/ml) in the presence and absence of penicillamine, hypotaurine and epinephrine (phe), and 4 sperm concentrations (1 x 10(6), 2 x 10(6), 3 x 10(6) and 4 x 10(6) /ml) were studied using 3202 buffalo oocytes. the mean proportions of fertiliz ...199316727261
in vitro maturation and fertilization of buffalo oocytes (bubalus bubalis ): effects of media, hormones and sera.media (tcm-199 and ham's f-10); sera (fetal calf serum, fcs, and buffalo estrous serum, bes); and hormones (fsh, 0.5 ug/ml, lh, 5 ug/ml and estradiol 1 ug/ml) were tested to determine the efficiency of in vitro maturation and fertilization of buffalo follicular oocytes. immature good quality cumulus-oocyte complexes (cocs) were randomly assigned to 1 of 4 experiments. each experiment consisted of 6 treatment groups. oocytes cultured for 24 hours in medium (tcm-199 or ham's f-10) containing 10% f ...199316727284
a simplified laparoscopy technique for repeated ovarian observation in the water buffalo (bubalus bubalis).a simplified technique of laparoscopy was developed for ovarian observation in the riverine buffalo, through a right paralumbar incision. the technique differed from previously described ones in that it involved only a single puncture and required no abdominal insufflation. a hopkins 0 degrees forward viewing endoscope (5.5 mm x 500 mm) in combination with an endoscope sheath having a built-in instrument channel, and a long flexible forceps (630 mm) were used. of the 23 observation attempts on 1 ...199316727332
malignant catarrhal fever in a swamp buffalo (bubalus bubalis) calf in new zealand.malignant catarrhal fever was diagnosed in a newly imported swamp buffalo calf. this disease is prevalent in deer and cattle in new zealand and causes sporadic losses in these species. the disease is recognised as a problem in buffaloes overseas and this paper records the first case in a swamp buffalo in new zealand.199316031692
assignment of genes to chromosome 4 of the river buffalo with a panel of buffalo-hamster hybrid cells.summary: to identify the river buffalo chromosome carrying the genes coding for gapd, tpi1, and ldhb, karyotypic examination was carried out on 14 buffalo-hamster hybrid clones previously tested for presence of this syntenic group. in cattle, this group (u3) has been assigned to chromosome 5, which is assumed to be homologous to the long arm of buffalo chromosome 4. chromosome 4 was present in all five clones expressing the three enzymes, and absent in all seven negative clones, indicating that ...199321395717
in vitro fertilization of water buffalo follicular oocytes and their ability to cleave in vitro.water buffalo (murrah) oocytes were collected from ovaries obtained from a local slaughterhouse. they were classified according to the character of the cumulus cells under a stereomicroscope and then cultured in 25 mm hepes buffered tissue culture medium-199 (tcm-199) supplemented with 5% estrous water buffalo serum in an atmosphere containing 5% co2 in air at 39 degrees c. after 20 to 24 hours of in vitro maturation, the oocytes were cultured at 38.5 degrees c in tcm-199 supplemented with 1% es ...199216727214
ultrastructure and innervation of water buffalo (bubalus bubalis) seminal vesicle.the lining epithelium of secretory end pieces and central glandular duct in the seminal vesicle of the water buffalo (bubalus bubalis) consists of columnar principal and small polymorphous basal cells. a system of intercellular and even intracellular canaliculi enlarges the secretory surface. the most prominent organelle of the columnar principal cells is the granular endoplasmic reticulum, forming large aggregates of parallel lamellae. using antibodies against the neural cell adhesion molecule ...199210457788
buffalo and bali cattle--exploiting their reproductive behaviour and physiology.water buffalo (bubalus bubalis) and bali cattle (bos sondaicus) occupy production niches in much of the developing world's agricultural systems which in the developed world are occupied by bos indicus and bos taurus. both the former species are better-adapted to these environments and systems. both depend on population survival strategies different from each other's and from those of b. indicus and b. taurus. water buffalo rely on longevity and late sexual maturity, while bali cattle rely on hig ...19921304664
an evaluation of serologic tests used to diagnose brucellosis in buffaloes (bubalus bubalis).five serologic procedures were evaluated in buffaloes and results compared with cultures of 64 udder secretion specimens. the rivanol test was superior to all other methods in specificity. the card, rivanol and complement fixation tests identified all infected cows. many sera had complement fixing antibodies while agglutinins were absent. it was concluded that these were false positive results. a new procedure, the particle concentration fluorescence immunoassay, was a sensitive screening test b ...19921306917
disposition kinetics of gentamicin following repeated parenteral administration in buffalo calves (bubalus bubalis).disposition kinetics of gentamicin was determined in buffalo calves following repeated parenteral administration of 5 mg/kg body weight. the absorption (t1/2 ka) and elimination half-life (t1/2 beta) were found to be 0.40 +/- 0.12 and 4.33 +/- 0.39 h, respectively. statistical comparison of the values of pharmacokinetic determinants generated in this study with the corresponding values following single intramuscular injection at the same dose level as reported earlier by garg and garg, 1990, rev ...19921340000
a simple staining procedure for detecting the true acrosome reaction in buffalo (bubalus bubalis) spermatozoa.a simple dual staining procedure for detecting the true acrosome reaction in dried smears of buffalo spermatozoa is described. trypan blue is used first to differentiate live from dead spermatozoa and the dried smears which have been prepared are stained with giemsa for acrosome evaluation. four categories of spermatozoa were recognized: a) live, intact acrosome (acrosome pink, postnuclear cap clear); b) dead, intact acrosome (acrosome pink, postnuclear cap blue); c) live, detached acrosome (acr ...19921377498
in vitro fertilization of follicular oocytes from the swamp buffalo (bubalus bubalis) and kedah-kelantan cattle (bos indicus) in malaysia. 19921391198
detection of a sperm-coating antigen in the semen of bubalus bubalis.1. surface antigens of b. bubalis spermatozoa were solubilized by triton x-100 and edta; the sperm extract was used to raise antibodies in rabbits. 2. two major polypeptides, immunoprecipitated from the seminal plasma by the antibodies against the sperm extract, exhibited the same electrophoretic mobilities of two immunorelated sperm surface antigens. 3. the two polypeptides were isolated from the seminal plasma, by a multi-step chromatographic procedure, and found subunits of a single protein ( ...19921395507
plasma hormonal and electrolyte alterations in cycling buffaloes (bubalus bubalis) during hot summer months.plasma levels of progesterone, prolactin, luteinizing hormone, and electrolytes were monitored by radioimmunoassay in ten cycling buffaloes maintained at punjab agricultural university, ludhiana during the hot summer months of june-july. the plasma progesterone concentration ranged from 0.28 +/- 0.04 to 3.09 +/- 0.03 ng/ml at various stages of the oestrous cycle. prolactin values ranged from 319 +/- 23 to 371 +/- 25 ng/ml and lh levels from 0.95 +/- 0.05 to 1.35 +/- 0.08 ng/ml. concentrations di ...19921399107
high-resolution gtg-banding of chromosomes in the swamp buffalo (bubalus bubalis l.): description of chromosome 1. 19921399708
combined sarcocystis and gram-positive bacterial infections. a possible cause of segmental enterocolitis in thailand.between 1981 and 1990, 22 intestinal specimens surgically resected due to segmental enterocolitis were collected and examined. grossly, the specimens were classified into 3 groups 1) acute inflammation with hemorrhage and necrosis 2) constrictive lesion 3) false diverticulum with perforation. mostly, there was unisegmental involvement, distributed in jejunum, ileum and ileocolon. microscopically, small parasitic structures, interpreted to be unconventional excystation stage of sarcocystis homini ...19921402481
pharmacokinetics following a single intravenous administration and a dosage regimen for sulfadoxine in buffalo calves (bubalus bubalis). 19921413482
some glucose metabolic enzymes in various fractions of sarcocysts of sarcocystis fusiformis of buffalo (bubalus bubalis).a comparative biochemical study on some enzymes of glycogenolysis, glycolysis and the hexose monophosphate shunt pathway in various fractions (cyst wall, cyst fluid and zoites) of the sarcocysts of sarcocystis fusiformis from the oesophageal muscles of naturally infected indian water buffalo (bubalus bubalis) was carried out. the pattern and the magnitude of enzymic activity differed markedly in these fractions. phosphorylase, hexokinase, aldolase and pyruvate kinase showed their highest levels ...19921441191
the effect of long-term exposure to phosphamidon on the ruminal microorganisms and circulating carboxylesterase of bubalus bubalis.the effect of long-term exposure to the organophosphate insecticide phosphamidon on the ruminal microorganisms and serum carboxylesterase of buffalo calves was investigated. oral administration of phosphamidon in doses of 0.25 and 0.5 mg/kg per day for 120 days caused significant inactivation of carboxylesterase activity (16-32%) without eliciting any intoxicating signs apart from mild intermittent diarrhoea in the animals receiving the higher dose. the higher dose also produced a significant re ...19921466149
conjugal transfer of plasmid-borne bacteriocin production in enterococcus faecalis 226 nwc.enterococcus faecalis 226 nwc, isolated from natural whey cultures utilized as starter in water-buffalo mozzarella cheese manufacture, produces a bacteriocin, designated enterocin 226 nwc, which is inhibitory to listeria monocytogenes. plasmid analysis of e. faecalis 226 nwc showed a single 5.2-kb plasmid, pef226. in conjugation experiments, pef226 was transferred into a plasmid-free strain of e. faecalis jh2-2. the transfer required direct cell-to-cell contact and was not inhibited by dnase. th ...19921468612
disposition kinetics and dosage regimen of sulfapyridine in buffalo (bubalus bubalis).the disposition kinetics and dosage regimen of sulfapyridine were studied in buffalo calves following a single intravenous dose of 100 mg/kg. distribution half-life (t1/2 alpha) elimination half-life (t1/2 beta) and vd (area) was 0.181 +/- 0.008 h, 13.4 +/- 0.52 h and 0.59 +/- 0.03 l kg-1, respectively. total body clearance, which represents the sum of all clearance processes, and tissue/plasma (t/p) ratio were calculated to be 31.1 +/- 2.28 ml kg-1 h-1 and 2.25 +/- 0.09, respectively. a satisfa ...19921476083
immunoreactive prolactin, progesterone and luteinizing hormone in the seminal plasma of buffalo (bubalus bubalis).the presence of immunoreactive prolactin, luteinizing hormone (lh) and progesterone in buffalo seminal plasma is reported for the first time. correlations were obtained between various semen attributes and the levels of the above immunoreactive hormones. statistically significant (p < 0.01) negative correlations were found between the levels of immunoreactive prolactin in semen and sperm motility and viability. the coefficient of multiple linear correlation (r2) between the levels of immunoreact ...19921476086
effect of dietary amino acids on in vitro rumen bacterial protein synthesis in buffaloes.the effect of different ratios of urea to amino acid n at a fixed concentration of soluble sugars as energy source and varying levels of soluble sugars at optimum urea cell suspension was obtained from the rumen fluid of buffalo (bubalus bubalis) calves fed on a growth ration. under glucose fermentation, the bacterial protein content of the incubation mixture (i. m.) was increased to 3.91, 6.31 and 5.08 times the control value (urea alone) when 25, 50 and 75% of urea-n was replaced with amino ac ...19921476088
a 49, xo sterile murrah buffalo (bubalus bubalis). 19921496757
effects of oximes and atropine on acute phosphamidon intoxication in bubalus bubalis. 19921498478
river buffalo (bubalus bubalis l.) aa phenotype haemoglobins: characterization by immobiline polyacrylamide gel electrophoresis and high performance liquid chromatography and determination of the primary structure of the constitutive chains by mass spectrometry.1. polyacrylamide gel electrophoresis in ultra-narrow immobilized ph gradient shifted the "hb fast" band of aa buffalo phenotype haemoglobin into two components which were named hb1 and hb3. 2. urea/triton electrophoresis and reversed-phase hplc demonstrated that hb1 and hb3 differ in the presence of two structurally distinct alpha chains (alpha 1 and alpha 3), also suggesting that the alpha chains must differ for neutral amino acid substitution. 3. extensive mass spectrometric analysis on sever ...19921499282
effects of daily phosphamidon administration on certain blood biochemical measurements in bubalus bubalis. 19921504517
in vitro maturation, fertilization and development of follicular oocytes from buffalo (bubalus bubalis).cumulus-oocyte complexes, 5596, were cultured for 24 h in either tcm-199 or ham's f-10 with or without gonadotrophins and supplemented with either 20% buffalo oestrous serum (bes) or fetal calf serum (fcs). the maturation rates of oocytes cultured in tcm-199 or ham's f-10 medium supplemented with 20% bes were 47.4 +/- 17.8 and 44.8 +/- 25.6, respectively. addition of luteinizing hormone (lh) (5 micrograms ml-1) significantly improved the maturation rate in the ham's f-10 medium supplemented with ...19921518014
evolution of ruminant hemoglobins. thermodynamic divergence of ox and buffalo hemoglobins.the ligand-binding properties of hemoglobins from two homozygote phenotypes (aa and bb) of water buffalo (bubalus bubalis) have been characterized by equilibrium and kinetic techniques. in the case of the bb phenotype, the two constituent hemoglobins have been purified and separately analysed. buffalo hemoglobins display the reduced sensitivity to organic phosphates characteristic of ruminant hemoglobins, their physiological effector probably being the chloride ion. in contrast to the other know ...19921541266
studies on activation and levels of haemolytic complement of buffalo (bubalus bubalis). ii. alternate complement pathway activity in serum.buffalo serum caused lysis of unsensitized red blood cells (rbc) of sheep, goat, rabbit and guinea-pig. there was minimal lysis of cattle rbc, and homologous rbc were resistant. lysis of sheep and goat rbc was the result of natural antibodies as adsorption with respective rbc and addition of 8 mmol ethylene glycolbistetraacetate (egta) in diluent completely abrogated the haemolytic activity. the lysis of guinea-pig and rabbit rbc was only partially decreased by these treatments, indicating the p ...19921546445
psychoactive mushroom use in koh samui and koh pha-ngan, thailand.this paper presents the results of recent ethnomycological exploration in southern thailand. field observations, interviews and collection of fungi specimens were carried out primarily on two islands, koh samui and koh pha-ngan, situated in the western region of the gulf of siam. some fieldwork was also conducted in the northern thai province of chiang dao and in the southern thai province of surat thani. during five separate excursions (1989-90), observations were made of occurrence, harvesting ...19921548895
a comparative study of the temperature dependence of the oxygen-binding properties of mammalian hemoglobins.the effect of temperature on the oxygen-binding properties of hemoglobin (hb) from ruminants, such as ox, reindeer, musk ox, mouflon and egyptian water buffalo is compared to that of human adult hb (hba). a striking difference emerges where in the presence of chloride ions and in the absence of 2,3-diphosphoglycerate [gri(2,3)p2] a strongly reduced exothermic oxygenation process is observed for all ruminant hb investigated with respect to hba. next, in the presence of physiological concentration ...19921551393
around the world with 180 million water buffalo. 19921568893
an ultrastructural study of the sertoli cell in the water buffalo (bubalus bubalis).the ultrastructure of sertoli cell in the water buffalo (bubalus bubalis) was observed in a transmission electron microscope. the nucleus had homogeneous nucleoplasm, scarce heterochromatin and multivesicular nuclear body (mnb). the mnb was composed of numerous vesicles and ribosome-like dense structures. the vesicles varied in size and number and contained a sparse and flocculent substance. in the indentation of the nucleus, aggregates of ribosomes were frequently observed. in the apical and mi ...19921585996
quantitative morphology of the testicular tubular epithelium in the water buffalo (bubalus bubalis).ultrastructural features and morphometric evaluations of water buffalo seminiferous epithelium are reported for the 6 phases of the spermatogenic cycle. the relative sertoli cell volume varies between 30% (phase 4) and 39% (phase 8), the calculated volume of a sertoli cell between 7118 microns3 and 8968 microns3 (phase 4). smooth er is the organelle that exhibits the most prominent changes in sertoli cells during the spermatogenic cycle: it occupies about 6% in phase 3 and 21% in phase 4. all sp ...19921605402
comparison of the hemoglobins of the platyhelminths gastrothylax crumenifer and paramphistomum epiclitum (trematoda: paramphistomatidae).1. gastrothylax crumenifer and paramphistomum epiclitum parasitize the water buffalo bubalus bubalis. 2. gastrothylas hemoglobin consisted of two fractions of ca 30,000 and ca 18,000 by gel filtration. sds-electrophoresis showed both to be single, ca 15,000 chains. 3. paramphistomum hemoglobin was ca 16,000 by both gel filtration and sds-electrophoresis. 4. reversed-phase chromatography of carboxymethylated trematode and buffalo globins gave single peaks and two peaks, respectively. although par ...19921611885
a new technique for the precise location of lactate and malate dehydrogenases in goat, boar and water buffalo spermatozoa using gel incubation film.gel incubation film, which contained gelatin to prevent the diffusion of enzyme during chemical reaction and phenazine methosulfate to operate as a hydrogen acceptor between nadh and tetrazolium, was used and light microscopy revealed that lactate dehydrogenase was located in the head and tail of the spermatozoa as well as in the midpiece, whereas malate dehydrogenase was confined to the midpiece in spermatozoa of the animals examined. in goat spermatozoa, lactate dehydrogenase was associated ma ...19921625237
hematological study of water buffalo (bubalus bubalis) during theileriosis (theileria annulata). 19921626870
influence of different sources of injected selenium on certain enzymes, glutathione and adenosylmethionine concentration in buffalo (bubalus bubalis) calves.sodium selenite and selenomethionine were investigated as possible causative factors for the induction of degnala disease syndrome in twelve buffalo (bubalus bubalis) calves divided into three groups of four. group 1 was the control group and received no additional selenium. sodium selenite and selenomethionine were given daily as intramuscular injections on a selenium-equivalent basis, with a weekly increment in the dose of 0.05 mg se/kg live weight from 0.05 to 0.20 mg se/kg live weight per da ...19911662070
serotype o157:h7 escherichia coli from bovine and meat sources.serotype o157:h7 escherichia coli strains from several different bovine and meat (beef) sources were studied to determine the diversity of their virulence properties and to compare their plasmid characteristics. eighteen strains from cattle feces, 2 from water buffalo feces, 3 from beef samples, and 2 from feces of human hemolytic uremic syndrome cases were examined. all of these strains hybridized with the cvd419 dna probe which identifies serotype o157:h7 and many other serotypes of verocytoto ...19911677942
disposition kinetics of gentamicin in buffalo calves (bubalus bubalis) following single intravenous administration. 19911744943
effect of some electrolytes on in vitro rumen microbial protein synthesis in buffaloes.in vitro studies were carried out to examine the effect of mncl2, mgcl2, cocl2 and cacl2 on protein synthesis by rumen microorganisms obtained from fistulated buffalo calves (bubalus bubalis). the concentration of the electrolytes in stained rumen fluid (srf) ranged from 1 to 20, 1 to 20, 0.25 to 5 and 1 to 20 mm, respectively. mncl2 (15 mm), mgcl2 (10 mm), cocl2 (2.5 mm) and cacl2 (20 mm) increased the protein content in the incubation mixture (im) by 501, 230.8, 537.6 and 84% and the per cent ...19911750368
electrophoretic and chromatographic evidence for allelic polymorphisms in the river buffalo alpha-globin gene complex.isoelectric focusing in the ultranarrow immobilized (7.1-7.5) ph gradient (ipg) of hemoglobin and high-performances liquid chromatography (hplc) of globin chains were used to investigate hb polymorphism in italian river buffalo. six different phenotypes, each characterized by two or four different hbs, were detected by ipg, whereas two different ii alpha-globin chains were separated from two different i alpha-chains by hplc. two alpha-chains (i alpha 1 and ii alpha 3), and hbs with similar mobil ...19911772399
effect of different seasons on concentration of plasma luteinizing hormone and seminal quality vis-à-vis freezability of buffalo bulls (bubalus bubalis).seasonal variations in semen quality, freezability and plasma luteinizing hormone (lh) levels were studied between summer and spring. semen volume, density and initial sperm motility did not differ significantly between different seasons. plasma lh decreased between summer and spring but the differences were, however, not significant. pre-freezing motility did not differ significantly but post-freezing motility varied significantly (p less than 0.01) between seasons. post-freezing motility was l ...19911778653
trypanosoma evansi infection in worked and unworked buffaloes (bubalus bubalis) in indonesia.the effect of controlled amounts of exercise on the outcome of trypanosoma evansi infection was studied in groups of swamp buffaloes (bubalus bubalis) experimentally infected with t. evansi. daily body temperature, packed cell volume (pcv) and parasitaemia measurements were obtained from each animal for up to 110 days after infection. exercise did not appear to exacerbate the effect of t. evansi infection in that similar temperature, pcv and parasitaemia profiles were obtained with both exercise ...19911788927
evidence for the presence of two different beta-globin chains in the hemoglobin of the river buffalo (bubalus bubalis l.).1. hemoglobin (hb) of the river buffalo (bubalus bubalis l.) was studied by employing isoelectric focusing (ief) in the 6.7-7.7 ph range and by ief in ultra-narrow immobilized ph gradient (ipg) 7.1-7.5. 2. three hb bb phenotypes were identified which were characterized by sets of two or four hbs with different isoelectric points. 3. these type were called bb, bsbs and bbs, the bs phenotype showing hbs with slightly slower mobility. 4. analysis of constituent globin chains by acid urea polyacryla ...19911790681
quantitative aspects of water buffalo (bubalus bubalis) spermatogenesis.in the buffalo, seminiferous tubules occupy about 82% of the testis. spermatogenesis can be divided into 6 stages according to characteristic cellular associations in the seminiferous epithelium. a-spermatogonia have a volume of approximately 1,400 microns3 and the highest absolute mitochondrial volume of all spermatogenic cells. b-spermatogonia display cellular, nuclear and mitochondrial volumes of approximately half the values of a-spermatogonia. from preleptotene (approximately 470 microns3) ...19911793664
partial purification and isoelectric focusing patterns of the buffalo (bubalus bubalis) and the kedah-kelantan cattle (bos indicus) glutathione transferases.1. glutathione transferases from the liver, lung and kidney tissues of the buffalo (bubalus bubalis) and the kedah-kelantan cattle (bos indicus) were partially purified by ammonium sulphate precipitation and sephadex g-75 gel filtration. 2. liver tissue contains the highest enzyme activity when compared to the lung and kidney tissues. 3. the activity in cattle is higher than that in the buffalo. 4. isoelectric focusing separates the activities into the acidic, near neutral and basic fractions. 5 ...19911799968
cleavage capability of water buffalo follicular oocytes classified by cumulus cells and fertilized in vitro.water buffalo (murrah) oocytes were collected from ovaries obtained from the slaughter house. they were classified according to the character of the cumulus cells under a stereomicroscope, and cultured in 25 mm hepes buffered tissue culture medium-199 (tcm-199) supplemented with 5% estrous water buffalo serum in an atmosphere containing 5% co2 in air at 39 degrees c. after 20-24 hr of in vitro maturation, the oocytes were fertilized using capacitated sperm obtained from 4 different bulls. for cl ...19911832035
seroepidemiology of bivens arm virus infections of cattle in florida, st. croix and puerto rico.bivens arm virus (bav) is a newly discovered rhabdovirus infecting cattle and water buffalo in florida. the virus is classified as a member of the tibrogargan group, members of which have hitherto been found only in australasia. they are considered to be transmitted by culicoides species. bivens arm virus was first isolated from culicoides insignis which suggests that bav is also transmitted by this genus. a serological survey of two small groups of cattle raised in st. croix and puerto rico, in ...19911871952
pharmacokinetics of cephaloridine in bubalus bubalis. 19911920608
physico-chemical and immunological characteristics of pituitary prolactin from water buffaloes (bubalus bubalis).prolactin (prl) was purified from freshly frozen pituitary glands of water buffaloes (bubalus bubalis) by a combination of existing procedures of ellis and jiang and wilhelmi involving serial extraction of different pituitary proteins. the partially purified preparation was further fractionated on deae-sephadex followed by sephadex g-100 chromatography. this was finally purified on hplc. this preparation was found to be homogeneous by sds-page and hplc and had a single n-terminus amino acid (thr ...19911922008
ethnopharmacology of rhinoceros horn. ii: antipyretic effects of prescriptions containing rhinoceros horn or water buffalo horn.aqueous extracts of rhinoceros horn or water buffalo horn demonstrated significant antipyretic action at 2.5 g/ml i.p. (1 ml/animal) in rats with hyperthermia induced by subcutaneous injection of turpentine oil. qingying decoction, a classic compound prescription composed of rhinoceros horn and eight herbs, showed significant antipyretic action at dosages equivalent to 0.5 g/ml of rhinoceros horn extract. comparable action was obtained by qingying decoction prepared with water buffalo horn. it i ...19911943172
preliminary observations on the epidemiology of bovine ephemeral fever in china.bovine ephemeral fever (bef) is a viral disease of cattle and water buffalo recognised for the first time in china in 1955. the disease has been endemic in south china with 13 epidemics since 1955, seven epidemics reaching central china and one extending into north-east china (40 degrees n). the outbreaks have been seasonal with all occurring in the warmer summer/autumn months of june to october when possible insect vectors are active. reports of clinical disease were confirmed as bef by the det ...19912038767
pharmacokinetics and urinary excretion of gentamicin in bubalus bubalis calves following intramuscular administration.the pharmacokinetics and urinary excretion of gentamicin was studied in buffalo calves after a single intramuscular administration (10 mg kg-1). kinetic determinants were calculated by using a two compartment open model. the absorption (t1/2ka) and biological half lives (t1/2 beta) were calculated to be 0.43 +/- 0.08 and 3.79 +/- 0.23 h, respectively. the value of the apparent volume of distribution (vdb) was found to be 0.38 +/- 0.07 litre kg-1. the satisfactory intramuscular dosage regimen of ...19912047583
temperature modulation of bovine hemoglobins.the functional properties of hemoglobin from egyptian water buffalo have been characterized as a function of ph, temperature and chloride concentration. alongside overall similarities shared with ox and arctic ruminant hemoglobins, hemoglobin from buffalo shows significant differences with respect to the effect of temperature. the results obtained may suggest that the limited effect of temperature on oxygen binding recently reported for ox hemoglobin could be regarded as an interesting case of a ...19912059223
the use of the probit model to predict pregnancy status of buffalo based on physio-chemical properties of estrual mucus.improper timing of artificial insemination with respect to ovulation is one of the major factors hampering the conception rate in buffalo. the present study was an attempt to relate physio-chemical changes in estrual mucus to subsequent pregnancy status in order to find their optimal values for determining the time for artificial insemination (ai). serum estradiol, total protein and dry matter contents of estrual mucus were evaluated to predict the subsequent pregnancy in 36 buffalo during octob ...199116726984
community structure affects behavior.aid's prevention efforts can benefit from taking into account 5 main aspects (kepra) of community structure identified by anthropologists: 1) kinship patterns, 2) economics, 3) politics, 4) religion, and 5) associations. for example, in uganda among the basoga and paternal aunt or senga is responsible for female sex education. such culturally determined patterns need to be targeted in order to enhance education and effectiveness. economics can reflect differing systems of family support thro ...199112316890
superovulatory response of river bufalo (bubalus bubalis ).the ovarian response of 25 buffalo-cows was visually assessed, and their oviducts and uteri separately flushed 3 to 6 d post superovulatory estrus at slaughter. ten buffalo-cows slaughtered on days 5 and 6 were examined per rectum for corpora lutea (cl) and follicles > 8 mm prior to slaughter, and the estimate was compared later with the actual ovarian response. five out of the ten buffalo-cows were nonsurgically flushed in vivo on day 5 of the estrous cycle, a day before slaughtering, and as a ...199016726742
effect of uterine fluid on in vitro acrosome reaction of buffalo (bubalus bubalis ) spermatozoa.the present study was undertaken to examine in vitro acrosome reaction in the uterine fluid of estrous buffalo. successful acrosome reaction was achieved by incubating buffalo spermatozoa in 2% detoxified uterine fluid in biggers whitten whittingham (bww) medium, ph 7.4 at 37 degrees c and a sperm concentration of 36 x 10/ml. neat uterine fluid has been found to be toxic to spermatozoa, hence we detoxified the uterine fluid at 56 degrees c for 30 min, which resulted in higher percentage of sperm ...199016726752
monosomy x and gonadal dysgenesis in a buffalo heifer (bubalus bubalis).investigations on a 3-yr-old river buffalo heifer presenting anestrus revealed a chromosome make-up of 2n=49 in the lymphocyte cultures, compared with the 2n=50 characteristic of riverine buffalo. the missing chromosome was identified as one of the xs by karyotypic analysis, and monosomy x was confirmed by c and g-banding techniques. both ovaries of the heifer were underdeveloped, although the other components of the internal genitalia were normal. the phenotypic and karyotypic features confirme ...199016726819
endocrinological evaluation of the induction of superovulation with pmsg in water buffalo (bubalus bubalis).ten nonlactating buffalo were superovulated with 3000 iu pmsg. luteolysis was induced with 500 microg cloprostenol (pg) 60 and 72 h after pmsg. five buffalo were alloted for natural mating and five were bred by artificial insemination 60 and 84 h after the first pg treatment. since four buffalo developed pyometra, only 6 of 10 underwent embryo collection successfully 180 to 190 h after pg. three buffalo yielded only one morula each, while the remaining three yielded a total of two, three and fou ...199016726846
lactate dehydrogenase in two digenetic trematodes and their host.polyacrylamide gel electrophoresis of the two digenetic trematodes, gigantocotyle explanatum from the liver and gastrothylax crumenifer from the rumen of the water buffalo, bubalus bubalis revealed the presence of at least six and seven isoenzymes of lactate dehydrogenase (ldh), respectively in a partially purified enzyme preparation. the respective host tissues showed five isoenzymes of ldh, which are characteristic to the vertebrates. both parachloromercuribenzoate and iodoacetate affected the ...19902074142
yersinia pseudotuberculosis infection of buffaloes (bubalus bubalis). 19902090274
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