Publications

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salmonella, escherichia coli o157:h7 and e. coli biotype 1 in a pilot survey of imported and new zealand pig meats.a pilot survey for the pathogens salmonella and escherichia coli o157:h7, and e. coli biotype 1 was conducted on 100 new zealand-produced (domestic) pig carcasses and 110 imported pig meat samples over an 8-month period to assess the likelihood of introduction of novel pathogen strains into new zealand (nz), and as a guide for development of a domestic pork national microbiological database programme. salmonella was not isolated from domestic pig carcasses or from pig meat imported from canada a ...200919171260
time dependent vascular and myocardial responses of a second generation, small vessel, paclitaxel-eluting stent platform.the purpose of this study was to evaluate the time-dependent vascular and myocardial responses to a second-generation, small vessel (< or =2.5 mm) paclitaxel-eluting stent (pes) platform.200919180652
population dynamics of swine influenza virus in farrow-to-finish and specialised finishing herds in the netherlands.influenza virus infections with subtypes h1n1, h3n2 and h1n2 are very common in domestic pigs in europe. data on possible differences of population dynamics in finishing pigs in farrow-to-finish herds and in specialised finishing herds are, however, scarce. the presence of sows and weaned piglets on the same premises may, however, affect the exposure of finishing pigs to influenza viruses. in a longitudinal study on 14 farrow-to-finish herds and 15 finishing herds, groups of pigs were followed b ...200919181461
open versus laparoscopic retroperitoneal lymph node dissection: assessing adequacy of dissection in a porcine model.laparoscopic retroperitoneal lymph node dissection (l-rplnd) is emerging as a viable alternative to traditional open retroperitoneal lymph node dissection (o-rplnd). despite numerous reports confirming clinical oncologic equivalency between the two approaches, however, concerns still remain over the adequacy of laparoscopic dissection. we therefore sought to compare the completeness of dissection between o-rplnd and l-rplnd in a porcine model.200919193133
initial experimental evaluation of wireless capsule endoscopes in the bladder: implications for capsule cystoscopy.cystoscopy remains one of the most important diagnostic procedures for the lower urinary tract. wireless capsule endoscopy was introduced in the 1990s but use to date is limited to gastroenterology.200919201077
retrograde flush following warm ischemia in the non-heart-beating donor results in superior graft performance at reperfusion.the use of non-heart-beating donors (nhbd) has been propagated as an alternative to overcome the scarcity of pulmonary grafts. the presence of postmortem thrombi, however, is a concern for the development of primary graft dysfunction. in this isolated lung reperfusion study, we looked at the need and the best route of preharvest pulmonary flush.200919201425
the house fly (musca domestica) as a potential vector of metazoan parasites caught in a pig-pen in germany.in the present study a total of 224 specimens of the synanthropic house fly (musca domestica) were caught in a pig-pen of an organic farmer in dormagen (germany). the flies were examined for their potential as a carrier of metazoan parasites. on the exoskeletons and in the intestines of the flies the eggs and/or larvae of four endoparasite nematode species of domestic pigs (ascaris suum, strongyloides ransomi, metastrongylus sp., undetermined strongylida) were isolated. also one ectoparasite spe ...200919081196
importance of the porcine adam3 disintegrin domain in sperm-egg interaction.in the mouse, adam3, a well-characterized testis-specific protein of the a disintegrin and metalloprotease (adam) family, has a crucial role in fertilization by mediating sperm binding to the egg zona pellucida. however, little is known about adam3 in other species, such as domestic pigs. we have identified porcine adam3 and analyzed the protein. rt-pcr and trypsinization of sperm surface proteins revealed that porcine adam3 is expressed at high levels in the testis and on the sperm surface. fur ...200919106482
molecular magnetic resonance imaging of deep vein thrombosis using a fibrin-targeted contrast agent: a feasibility study.to evaluate the value of a fibrin-specific mr contrast agent (ep-2104r; epix pharmaceuticals) for detection of deep vein thrombosis (dvt) and monitoring of percutaneous intervention for treatment.200919151606
pretreatment with low-energy shock waves induces renal vasoconstriction during standard shock wave lithotripsy (swl): a treatment protocol known to reduce swl-induced renal injury.to test the hypothesis that the pretreatment of the kidney with low-energy shock waves (sws) will induce renal vasoconstriction sooner than a standard clinical dose of high-energy sws, thus providing a potential mechanism by which the pretreatment sw lithotripsy (swl) protocol reduces tissue injury.200919154458
measurements of subcutaneous tissue po2 reflect oxygen metabolism of the small intestinal mucosa during hemorrhage and resuscitation. an experimental study in pigs.the first tissues to be deprived of perfusion and oxygenation in a hypovolemic situation and the last ones to be reperfused are the subcutaneous tissue and the gastrointestinal mucosa. we hypothesized that measurements of subcutaneous tissue gases and ph might reflect simultaneous changes in oxygenation of the intestinal mucosa. the aim of this study was to evaluate tissue gases and ph as measures of tissue oxygenation and tissue oxygen metabolism in subcutaneous and intestinal tissues simultane ...200919155629
expanding the versatility of cardiac pet/ct: feasibility of delayed contrast enhancement ct for infarct detection in a porcine model.it has recently been suggested that, similar to mri, ct can be used to detect infarcts at high resolution by delayed myocardial contrast enhancement. in cardiac pet/ct, this ability to detect infarcts may increase the versatility and integrative potential of pet and ct study components. we sought to determine the feasibility of delayed ct-enhancement in the pet/ct environment and compared it with pet-defined rest perfusion for the measurement of infarct size.200919164240
a novel tgf-beta antagonist speeds reepithelialization and reduces scarring of partial thickness porcine burns.scar formation after thermal injury is common and results in significant aesthetic and functional impairment. transforming growth factor beta (tgf-beta) plays a significant role in scar formation. we tested the hypothesis that a novel tgf-beta peptantagonist would reduce scar formation and wound contraction in partial thickness burns by using a randomized controlled experiment. the subjects include two domestic pigs (20-25 kg). forty burns were created on the animal's dorsum using an aluminum ba ...200919165091
changes in spatial collagen content and collagen network architecture in porcine articular cartilage during growth and maturation.the present study was designed to reveal changes in the collagen network architecture and collagen content in cartilage during growth and maturation of pigs.200918849174
effects of pre-arrest and intra-arrest hypothermia on ventricular fibrillation and resuscitation.hypothermia has been shown to improve survival and neurological outcomes for ventricular fibrillation (vf) cardiac arrest. the electrophysiological mechanisms of hypothermia are not well-understood, nor are the effects of beginning cooling during the resuscitation.200918952346
enhanced discrimination of african swine fever virus isolates through nucleotide sequencing of the p54, p72, and pb602l (cvr) genes.complete sequencing of p54-gene from 67 european, american, and west and east african swine fever virus (asfv) isolates revealed that west african and european asfv isolates classified within the predominant genotype i according to partial sequencing of p72 were discriminated into four major sub-types on the basis of their p54 sequences. this highlighted the value of p54 gene sequencing as an additional, intermediate-resolution, molecular epidemiological tool for typing of asfv viruses. we furth ...200919009341
comparative study of in vivo lymphatic sealing capability of the porcine thoracic duct using laparoscopic dissection devices.sealing the lymphatic vessels during abdominal and pelvic surgery is important to prevent the leakage of lymphatic fluid and its resultant sequelae. to our knowledge we compared for the first time the quality of lymphatic sealing by each of 4 commonly used laparoscopic dissection devices.200919010491
pig and guinea pig skin as surrogates for human in vitro penetration studies: a quantitative review.both human and animal skin in vitro models are used to predict percutaneous penetration in humans. the objective of this review is a quantitative comparison of permeability and lag time measurements between human and animal skin, including an evaluation of the intra and inter species variability. we limit our focus to domestic pig and rodent guinea pig skin as surrogates for human skin, and consider only studies in which both animal and human penetration of a given chemical were measured jointly ...200919013230
species identification of trichinella isolates from china.two species of trichinella were identified from china by means of pcr amplification of the mitochondrial small subunit ribosomal dna and the expansion segment v region of the ribosomal dna. seven isolates originating from domestic pig and one isolate originating from dog showed identical dna banding pattern to trichinella spiralis, and two isolates from dog showed dna banding pattern identical to trichinella nativa. sequence analysis of the 5s rdna inter-gene spacer region from the ten trichinel ...200919036522
pilot study evaluating ureteric physiological changes with a novel 'ribbon stent' design using electromyographic and giant magnetoresistive sensors.to test a novel 'ribbon stent' (rs) design using an extraluminal bipolar electromyographic (emg) and giant magnetoresistive (gmr) sensor system to characterize ureteric responses.200919040534
the birth of a trichinella britovi focus on the mediterranean island of sardinia (italy).for 60 years, the islands of the mediterranean basin were considered to be trichinella-free. in april 2005, an outbreak of human trichinellosis due to the consumption of infected pork involved 11 persons in the villages of orgosolo and lanusei (nuoro province) on the island of sardinia (italy). we conducted an investigation to identify free-range and backyard pigs and other humans with trichinella infection in the area of the 2005 outbreak. we also tested wild animals from various parts of sardi ...200919041178
desferrioxamine attenuates minor lung injury following surgical acute liver failure.acute liver failure (alf) can be complicated by lung dysfunction. the aim of this study was to test the hypothesis that inhibition of oxidative stress through iron chelation with desferrioxamine (dfx) attenuates pulmonary injury caused by alf. 14 adult female domestic pigs were subjected to surgical devascularisation of the liver and were randomised to a study group (dfx group, n = 7), which received post-operative intravenous infusion of dfx (14.5 mg x kg(-1) x h(-1) for the first 6 h post-oper ...200919047311
inhibition of complement and cd14 attenuates the escherichia coli-induced inflammatory response in porcine whole blood.the innate immune response is a double-edged sword in systemic inflammation and sepsis. uncontrolled or inappropriate activation can damage and be lethal to the host. several studies have investigated inhibition of downstream mediators, including tumor necrosis factor alpha (tnf-alpha) and interleukin-1beta (il-1beta). emerging evidence indicates that upstream inhibition is a better therapeutic approach for attenuating damaging immune activation. therefore, we investigated inhibition of two cent ...200919047409
trichinella pseudospiralis in pig from croatia.trichinella spiralis and trichinella britovi are species that are frequently found in domestic pigs and various sylvatic animals in croatia. during routine trichinoscopy, non-encapsulated larvae were detected in the muscle tissue of a domestic pig. artificial digestion revealed a larvae burden of 602 muscle larvae per gram of tissue. tissue section analysis confirmed the presence of non-encapsulated larvae. multiplex pcr identified the larvae as t. pseudospiralis. this observation is consistent ...200919054619
isolation and characterization of intestinal escherichia coli clones from wild boars in germany.our understanding of the composition of escherichia coli populations in wild boars is very limited. in order to obtain insight into the e. coli microflora of wild boars, we studied e. coli isolates from the jejunums, ileums, and colons of 21 wild boars hunted in five geographic locations in germany. ten isolates per section were subjected to clonal determination using pulsed-field gel electrophoresis. one representative isolate per clone was further investigated for virulence traits, phylogeneti ...200919060173
trichinella spp. infection in horses of romania: serological and parasitological survey.herbivorous animals are usually, by virtue of their diet, outside the major transmission cycles of trichinella spp. however, since 1975, the year of the first report of human trichinellosis caused by the consumption of infected horse meat, the domestic horse has appeared as a novel vector of trichinella spp. infection to humans, with 15 outbreaks documented in france and italy. romania, one of the main countries exporting horses into the european union (eu), experienced a dramatic increase of tr ...200919062194
spontaneous cardiac arrhythmias recorded in three experimentally- and drug-naive laboratory species (canine, primate, swine) during standard pre-study screening.the purpose of this study was to collect and estimate the general prevalence of spontaneous irregularities in cardiac rhythms in three standard laboratory animal species: beagle dog (n=2450), cynomolgus monkey (n=1563), and domestic swine (n=704) during standard preclinical screening.200919111938
first magnetic resonance imaging-guided aortic stenting and cava filter placement using a polyetheretherketone-based magnetic resonance imaging-compatible guidewire in swine: proof of concept.the purpose of this study was to demonstrate feasibility of percutaneous transluminal aortic stenting and cava filter placement under magnetic resonance imaging (mri) guidance exclusively using a polyetheretherketone (peek)-based mri-compatible guidewire. percutaneous transluminal aortic stenting and cava filter placement were performed in 3 domestic swine. procedures were performed under mri-guidance in an open-bore 1.5-t scanner. the applied 0.035-inch guidewire has a peek core reinforced by f ...200919115070
validation of a western blot for the detection of anti-trichinella spp. antibodies in domestic pigs.trichinellosis is a zoonotic disease in humans caused by trichinella spp. according to international regulations and guidelines, serological surveillance can be used to demonstrate the absence of trichinella spp. in a defined domestic pig population. most enzyme-linked immunosorbent assay (elisa) tests presently available do not yield 100% specificity, and therefore, a complementary test is needed to confirm the diagnosis of any initial elisa seropositivity. the goal of the present study was to ...200919130084
porcine reproductive and respiratory syndrome virus (prrsv) infection in wild boars.the porcine reproductive and respiratory syndrome (prrs) is one of the economically most important swine diseases worldwide. the virus can be spread by viraemic and persistent infected pigs. spread and infection are advanced in areas with high herd and population densities. as wild boars have been found seropositive for prrsv in germany, france, and the usa, exchange between wild and domestic pig populations may exist. however, comprehensive information on prrsv infection in wild boars is presen ...200919131188
drug-eluting balloon: very short-term exposure and overlapping.paclitaxel balloon coating has shown promising effects in inhibiting restenosis in initial clinical trials. the aim of the present study was to evaluate the influence of two critical features of drug-eluting balloon (deb) application - inflation time and increased dose due to overlapping balloons. fifty-six stainless steel stents were implanted in the left anterior descending and circumflex coronary arteries of 28 domestic pigs using a 1.2:1.0 overstretch ratio. stents were mounted on convention ...200919132209
epidemiology, diagnosis, treatment, and control of trichinellosis.summary: throughout much of the world, trichinella spp. are found to be the causative agents of human trichinellosis, a disease that not only is a public health hazard by affecting human patients but also represents an economic problem in porcine animal production and food safety. due to the predominantly zoonotic importance of infection, the main efforts in many countries have focused on the control of trichinella or the elimination of trichinella from the food chain. the most important source ...200919136437
diversification of porcine mhc class ii genes: evidence for selective advantage.the major histocompatibility complex (mhc) is an immunological gene-dense region of high diversity in mammalian species. sus scrofa was domesticated by at least six independent events over eurasia during the holocene period. it has been hypothesized that the level and distribution of mhc variation in pig populations reflect genetic selection and environmental influences. in an effort to define the complexity of mhc polymorphisms and the role of selection in the generation of class ii gene divers ...200919142631
contrasting mode of evolution at a coat color locus in wild and domestic pigs.despite having only begun approximately 10,000 years ago, the process of domestication has resulted in a degree of phenotypic variation within individual species normally associated with much deeper evolutionary time scales. though many variable traits found in domestic animals are the result of relatively recent human-mediated selection, uncertainty remains as to whether the modern ubiquity of long-standing variable traits such as coat color results from selection or drift, and whether the unde ...200919148282
greenlight (532 nm) laser partial nephrectomy followed by suturing of collecting system without renal hilar clamping in porcine model.to assess the feasibility of partial nephrectomy (pn) without renal hilar clamping using the 80-w greenlight (532 nm) laser with opening of the collecting system followed by its suture in a porcine model.200918502479
laparoscopic repair of inferior vena caval injury using a chitosan-based hemostatic dressing.vena caval injury is a rare but serious complication of laparoscopic surgery, and often requires conversion to an open procedure. the current study investigated whether a vena caval injury could be repaired with a chitosan dressing laparoscopically.200918585679
distinct subtypes of urinary bladder epithelial cells with inducible and non-inducible cytochrome p450 1a1.cultured primary porcine urinary bladder epithelial cells (pubec) represent an adequate and easy to handle in vitro system for studies of urothelial toxicity. pubec maintain in vivo-like metabolic activities and physiological functions. they express inducible cytochrome p4501a isoenzymes, which are of particular relevance, since they contribute to activation of bladder carcinogens. a possible drawback of pubec is their isolation from common domestic pigs that do not represent an inbred strain. i ...200918636242
detection and size differentiation of simulated tooth root defects using flat-panel volume computerized tomography (fpvct).our aim was to evaluate the capacity of flat-panel volume computerized tomography (fpvct) to enable the observer to detect and differentiate 3 different sizes of simulated tooth root defects in radiographs.200918602316
hosts and habitats of trichinella spiralis and trichinella britovi in europe.trichinella spiralis and trichinella britovi are the two most common species of trichinella circulating in europe. based on data provided to the international trichinella reference centre over the past 20 years (data referring to 540 isolates of t. spiralis and 776 isolates of t. britovi), we describe the host species and habitat characteristics for these two pathogens in europe. a geographical information system was constructed using administrative boundaries, a corine land cover (clc) map, and ...200918708065
manganese-enhanced mri of acute cardiac ischemia and chronic infarction in pig hearts: kinetic analysis of enhancement development.to assess infarction development in pig hearts, mn-enhanced and gd-enhanced mri were used. in domestic pigs (25-35 kg, n = 37), the first and second diagonal branches of the left anterior descending coronary artery were ligated to induce acute ischemia and infarction (ischemia+reperfusion) or chronic infarction of increasing duration (3- 28 days). ex vivo experiments were performed on hearts perfused in the langendorff mode with a 50:50 mixture of blood and krebs-henseleit buffer using a spin-ec ...200918756440
small intestinal submucosa as a bioscaffold for tissue regeneration in defects of the colonic wall.small intestinal submucosa (sis) has proved considerable regenerative capacity for repair of bowel wall defects at different locations. this study assesses the effectiveness of sis in the repair of defects at a gastrointestinal location with strong bacterial contamination.200918766420
identification and sequencing of remnant messenger rnas found in domestic swine (sus scrofa) fresh ejaculated spermatozoa.the existence of specific messenger rna remnants contained within freshly ejaculated spermatozoa was described in several species. those investigations, using high-throughput techniques to screen the population of transcripts in ejaculated spermatozoa, were limited to the probes which mostly derived from nucleic acids of testicular tissues of either human or mice. the objective of this study was to investigate mrna remnants from ejaculated spermatozoa of the domestic swine (sus scrofa), a valuab ...200918786788
in vivo evaluation of anodic tio2 nanotubes: an experimental study in the pig.because of their ability to mimic the dimensions of constituent components of natural bone and the possibility to serve as a gene and drug-delivery carrier, nanotubes seem to be a promising coating for medical implants. aim of this study was to investigate the effects of a tio(2) nanotube structured surface on periimplant bone formation in vivo when compared with an untreated standard titanium surface. twenty-five titanium implants covered with an ordered tio(2) nanotube layer with an individual ...200918780361
the use of composite meshes in laparoscopic repair of abdominal wall hernias: are there differences in biocompatibily?: experimental results obtained in a laparoscopic porcine model.in recent years, laparoscopic repair of abdominal wall hernias has become increasingly established in routine clinical practice thanks to the myriad advantages it confers. apart from the risk of intestinal damage following adhesiolysis, to date no information is available on the best way of preventing the formation of new adhesions in the vicinity of the implanted meshes. numerous experimental investigations, mainly conducted on an open small-animal model, have demonstrated the advantages of coa ...200918806942
detection of brachyspira hyodysenteriae, lawsonia intracellularis and brachyspira pilosicoli in feral pigs.feral pigs are recognized as being a potential reservoir of pathogenic microorganisms that can infect domestic pigs and other species. the aim of this study was to investigate whether feral pigs in western australia were colonized by the pathogenic enteric bacteria lawsonia intracellularis, brachyspira hyodysenteriae and/or brachyspira pilosicoli. a total of 222 feral pigs from three study-populations were sampled. dna was extracted from faeces or colonic contents and subjected to a previously d ...200918818030
endothelialization of nonapposed stent struts located over the origin of a side branch: results with different carbofilm-coated stents.to evaluate the degree of endothelialization of the nonapposed struts located at the ostia of side branches.200919490356
drug delivery at the aortic valve tissues of healthy domestic pigs with a paclitaxel-eluting valvuloplasty balloon.restenosis occurs invariably within 1 year following balloon valvulopasty in aortic valve stenosis. the mechanism of restenosis seems to involve a dynamic cellular component that could be a target for drug inhibition. we investigated the feasibility of local drug delivery at the aortic valve tissues of healthy pigs with a paclitaxel-eluting balloon.200919490359
closed-chest experimental porcine model of acute myocardial infarction-reperfusion.progress in cardiovascular regenerative medicine research requires the availability of appropriate experimental animal models that are as close to humans as feasible. our objective was to assess the validity of a porcine endovascular model of myocardial infarction and reperfusion.200919490947
enhanced external counterpulsation promotes growth cytokines-mediated myocardial angiogenesis in a porcine model of hypercholesterolemia.enhanced external counterpulsation (eecp) improves ischemia in patients with refractory angina pectoris, but the mechanism remains unclear. to explore the mechanisms of eecp action, we detected progenitor cells presenting any of the following markers cd34(+), cd29(+), and cd106(+).200919493469
a comparison of kidney oxygenation profiles between partial and complete renal artery clamping during nephron sparing surgery in a porcine model.to compare kidney oxygenation profiles between partial and complete renal artery clamping during nephron sparing surgery (nss) in a porcine model.200919497169
classification of worldwide bovine tuberculosis risk factors in cattle: a stratified approach.the worldwide status of bovine tuberculosis (btb) as a zoonosis remains of great concern. this article reviews the main risk factors for btb in cattle based on a three-level classification: animal, herd and region/country level. a distinction is also made, whenever possible, between situations in developed and developing countries as the difference of context might have consequences in terms of risk of btb. recommendations are suggested to animal health professionals and scientists directly invo ...200919497258
first report of bvdv circulation in sheep in argentina.pestiviruses are capable of infecting a wide range of animals within the order artyodactila. currently, the genus pestivirus includes bovine viral diarrhea virus 1 (bvdv-1) and 2 (bvdv-2), border disease virus (bdv), and classical swine fever virus (csfv). bvdv-1, bvdv-2 and bdv are able to cross species barrier to infect a wide range of hosts, whereas csfv is restricted to domestic pigs and wild boars. in argentina, 70% of cattle are seropositive to bvdv. although there were some serological st ...200919501923
lipid emulsion combined with epinephrine and vasopressin does not improve survival in a swine model of bupivacaine-induced cardiac arrest.this study sought to evaluate the efficacy of lipid emulsion in reversing bupivacaine-induced cardiovascular collapse when added to a resuscitation protocol that included the use of epinephrine and vasopressin.200919512878
selective versus nonselective arterial clamping during laparoscopic partial nephrectomy: impact upon renal function in the setting of a solitary kidney in a porcine model.laparoscopic partial nephrectomy has emerged as a standard of care for small renal masses. nevertheless, there remains concern over the potential for irreversible insult to the kidney as a result of exposure to warm ischemia. we aim to investigate the utility of selective segmental arterial clamping as a means to reduce the potential for ischemic damage to a solitary kidney during laparoscopic partial nephrectomy utilizing a porcine model.200919514812
toxoplasma gondii infection in wild boars (sus scrofa) bred in brazil.domestic pigs are considered to be important sources of toxoplasma gondii infection for humans. due to the increased consumption of wild boar meat in brazil, this species may also be an important source of the parasite. the objective of the present study was to detect t. gondii infection in 306 blood samples collected from wild boars bred in the state of são paulo, brazil. samples were analyzed using the modified agglutination test (mat), and 14 (4.5%) of them yielded positive results. modern br ...200919515494
association of intramyocardial high energy phosphate concentrations with quantitative measures of the ventricular fibrillation electrocardiogram waveform.quantitative measures of the ventricular fibrillation (vf) electrocardiogram (ecg) have been correlated with the success of rescue shocks, making them ideal measures for guiding resuscitative interventions. correlation of intramyocardial energy stores with the change in quantitative vf ecg measures would provide mechanistic insight into their utility. we sought to investigate the relationship between intramyocardial energy stores and four quantitative ecg measures.200919523740
from wild animals to domestic pets, an evolutionary view of domestication.artificial selection is the selection of advantageous natural variation for human ends and is the mechanism by which most domestic species evolved. most domesticates have their origin in one of a few historic centers of domestication as farm animals. two notable exceptions are cats and dogs. wolf domestication was initiated late in the mesolithic when humans were nomadic hunter-gatherers. those wolves less afraid of humans scavenged nomadic hunting camps and over time developed utility, initiall ...200919528637
endoscopic closure of large colonic perforations using an over-the-scope clip: a randomized controlled porcine study.perforation of the colon is considered to be one of the most serious complications of flexible endoscopy. the over-the-scope clip system (otsc) has previously been shown to close small colonic perforations effectively. the aim of this randomized controlled porcine study was to compare acute closure strengths between the novel 11-mm over-the-scope-clip and surgical closure for large colonic perforations.200919533550
animal farm: pig in the middle. 200919536212
characterization of the replication-competent porcine endogenous retrovirus class b molecular clone originated from korean domestic pig.xenotransplantation from pigs offers an opportunity to resolve the shortage of human organs. the porcine endogenous retrovirus (perv) cannot be eliminated because of its presence in the germline dna. three subgroups of the replication-competent perv (perv-a, perv-b, and perv-c) have been identified in pigs. we constructed a molecular clone of perv-b from a korean domestic pig bac clone containing perv genomes, and its replication competency was characterized in human cells. the pol region of per ...200919543822
investigation of blood typing method for seoul national university miniature pig.pig blood group antigens may be present on grafted tissue as 16 isoforms, including the major one, a substrance. seoul national university (snu) miniature pigs and domestic pigs were used in this study. polymerase chain reaction (pcr) was performed for the erythrocyte antigen a (eaa) gene, and reverse transcriptase-pcr for pig a transferase fucosyl transferase (fut) 1 and fut-2. the hemagglutination test was performed with murine monoclonal anti-a and anti-b antibodies (mab), and immunohistochem ...200919545757
cell-cell adhesion in the prelaminar region of the optic nerve head: a possible target for ionic stress.this paper aims to study the anterior surface of the optic nerve in relation to its ability to support a source of stress acting from the vitreous cavity. the intercellular junctions of the lining astrocytes mediated by cellular adhesion molecules (cams) may be the main targets for ionic stress.200919546602
natural orifice transluminal endoscopic surgery gastrotomy closure with an over-the-endoscope clip: a randomized, controlled porcine study (with videos).secure endoscopic closure of transgastric natural orifice transluminal endoscopic surgery (notes) access is of paramount importance. the over-the-scope clip (otsc) system has previously been shown to be effective for notes gastrotomy closure.200919559416
effect of natural or vaccine-induced porcine circovirus type 2 (pcv2) immunity on fetal infection after artificial insemination with pcv2 spiked semen.the objectives of this study were to determine if vaccination against porcine circovirus type 2 (pcv2) or previous pcv2 infection of the dam are sufficient to prevent fetal infection when dams are artificially inseminated with pcv2-spiked semen. nine sows (sus domestica) were allocated into three groups of three dams each: the pcv2 naïve negative control group 1 was artificially inseminated with extended pcv2 dna negative semen during estrus, whereas the extended semen used in the vaccinated gro ...200919559470
comparison of inflammatory response after implantation of sirolimus- and paclitaxel-eluting stents in porcine coronary arteries.although both sirolimus (cypher) and paclitaxel (taxus) drug-eluting stents have demonstrated efficacy and safety in clinical trials, human autopsy data have raised concerns about long-term healing and the potential for local inflammatory reactions.200919564562
the effect of anterior spinal fusion on spinal canal development in an immature porcine model.experimental study.200919564754
experimental comparison of the stapled intestinal anastomotic techniques.various techniques of stapled intestinal anastomoses are performed in gastroenterological surgery. little is known about which technique is optimal. the intestines of a domestic pig were used in this study. stapled intestinal anastomoses of three types-functional end-to-end anastomosis (fetea), stapled side-to-end anastomosis (stea), and stapled end-to-end anastomosis (eea)-were constructed using pig intestines. the times for constructing anastomoses, length of the completed anastomosed intestin ...200919579195
discovery of swine as a host for the reston ebolavirus.since the discovery of the marburg and ebola species of filovirus, seemingly random, sporadic fatal outbreaks of disease in humans and nonhuman primates have given impetus to identification of host tropisms and potential reservoirs. domestic swine in the philippines, experiencing unusually severe outbreaks of porcine reproductive and respiratory disease syndrome, have now been discovered to host reston ebolavirus (rebov). although rebov is the only member of filoviridae that has not been associa ...200919590002
global left ventricular function: assessment with dual-source ct versus conventional ventriculography in a porcine model.cardiac computed tomography (ct) and conventional biplane ventriculography are established methods for the determination of ventricular function. recently dual source ct was introduced for cardiac ct scanning providing a temporal resolution of 83 ms independent of the patient's heart rate. the purpose of this study was to assess the global left ventricular function using retrospectively ecg-gated dual source computed tomography (dsct) in comparison to conventional ventriculography.200919593940
the degree of resistance to freezing-thawing is related to specific changes in the structures of motile sperm subpopulations and mitochondrial activity in boar spermatozoa.the main aim of this work was to analyze the possible relationship between the structures of motile-sperm subpopulations and boar (sus scrofa domesticus) sperm resistance to freezability. for this purpose, 45 boar ejaculates were subjected to a standard freezing-thawing protocol, and afterwards they were classified into three groups, in accordance with their resistance to freezing-thawing. our analysis yielded four separate motile-sperm subpopulations in all of the studied ejaculates, both in fr ...200919604570
comparison of efficacy of pulsed biphasic waveform and rectilinear biphasic waveform in a short ventricular fibrillation pig model.the waveform designs and their relative defibrillation efficacy of external biphasic waveforms may differ remarkably among manufacturers. in this study, we compared pulsed biphasic waveform (pbw) with rectilinear biphasic waveform (rbw) and their effects on terminating ventricular fibrillation (vf).200919604618
pro-inflammatory, pleiotropic, and anti-inflammatory tnf-alpha, il-6, and il-10 in experimental porcine intervertebral disk degeneration.the aim of this study was to check the balance between tumor necrosis factor-alpha (tnf-alpha), interleukin-6 (il-6), and interleukin-10 (il-10) in well-developed end-stage disk disease in the disk itself as well as in paradiskal spine. in 6 domestic pigs the cranial bony end plate of the l4 vertebra was perforated to the nucleus pulposus. at 3 months the degenerated experimental and contiguous control disks, together with the adjoining bony and cartilaginous vertebral end plates, bone marrow, a ...200919605905
first record of pseudorabies in feral swine in nebraska.in 2007, two new populations of feral swine were discovered in nance and valley counties, nebraska, usa. necropsies and serologic testing was done on two individuals from the nance county herd. results indicated that a lactating sow had positive antibodies for pseudorabies virus (prv). investigations conducted by nebraska game and parks commission law enforcement division confirmed that the infected individual was transported illegally to nebraska, usa, from texas, usa. all domestic swine herds ...200919617504
transforming growth factor-alpha in a defined medium during in vitro maturation of porcine oocytes improves their developmental competence and intracellular ultrastructure.we examined the effects of transforming growth factor-alpha (tgf-alpha) to develop a defined medium for in vitro maturation (ivm) of porcine (sus scrofa domesticus) oocytes. cumulus-oocyte complexes (cocs) were matured in porcine oocyte medium containing 3mg/ml polyvinyl alcohol (pom) and tgf-alpha (0, 1, 10, or 100 ng/ml) in the presence or absence of the gonadotropins equine chorionic gonadotropin (ecg) and human chorionic gonadotropin (hcg). in the absence of gonadotropins, adding 10 ng/ml tg ...200919632712
transplantation of bone marrow-derived stem cells improves myocardial diastolic function: strain rate imaging in a model of hibernating myocardium.the aim of this study was to evaluate the cardioprotective effects of bone marrow-derived stem cells on myocardial compliance in a chronic ischemia model regarding strain rate (sr) parameters during dobutamine stress echocardiography (dse).200919647407
preferences of minipigs for environmental enrichment objects.the minipig is an increasingly popular species for use in behavioral and toxicologic studies. as a result, quantification of environmental enrichment preferences for this species is especially important. we exposed 6 individually housed prepubertal female yucatan minipigs to 1 of 3 different objects on a rotating schedule: 2 sessions with a hard plastic ball (diameter, 21.0 cm), and 3 sessions each with a large plastic apple (diameter, 22.5 cm) and a soft rubber cone (height, 48.0 cm). objects w ...200919653948
african swine fever: how can global spread be prevented?african swine fever (asf) is a devastating haemorrhagic fever of pigs with mortality rates approaching 100 per cent. it causes major economic losses, threatens food security and limits pig production in affected countries. asf is caused by a large dna virus, african swine fever virus (asfv). there is no vaccine against asfv and this limits the options for disease control. asf has been confined mainly to sub-saharan africa, where it is maintained in a sylvatic cycle and/or among domestic pigs. wi ...200919687038
healing of mid-dermal burns in a diabetic porcine model.wound healing is delayed in diabetic patients. we developed a diabetic-porcine burn model and compared the healing of partial-thickness burns in normal and diabetic pigs. we hypothesized that wound healing would be delayed in the diabetic swine. diabetes mellitus was chemically induced in three domestic pigs (25-50 kg) by intravenous injection of streptozotocin 130 mg/kg over 30 minutes. glucose levels were maintained between 250 and 500 mg/dl by injecting short-acting or long-acting insulin 1 u ...200919692921
frequent transmission of hepatitis e virus among piglets in farms in southern france.the present study aimed to assess whether hepatitis e virus (hev) is present in domestic pigs in southern france, and to determine the relationship between hev sequences detected from pigs and from humans. two hundred fifteen sera, 207 stools, and 107 bile samples were collected from 3- or 6-month-old pigs from different regions of southern france. pig igg anti-hev antibodies testing was performed using a commercial elisa kit with minor modifications. pig hev rna was tested by real-time pcr and ...200919697419
initial in vivo studies with a polymer-based mr-compatible guide wire.the purpose of this study was to evaluate a new polymer-based and magnetic resonance (mr) imaging-compatible guide wire for its application in mr-guided endovascular interventions, particularly for interventional peripheral mr angiography in swine experiments in vivo. a passive device tracking method tailored to the specific conditions of peripheral mr angiography was developed. near-real-time visualization of the guide wire was accomplished in vivo in the carotid artery, aorta, heart, and iliac ...200919699660
porcine cd18 mediates actinobacillus pleuropneumoniae apxiii species-specific toxicity.actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, produces apx toxins that are recognized as major virulence factors. recently, we showed that apxiiia-cytotoxic activity specifically targets sus scrofa leukocytes. since both ltxa from aggregatibacter actinomycetem comitans (aggressive periodontitis in humans) and lkta from mannheimia haemolytica (pneumonia in ruminants) share this characteristic, respectively towards human and ruminant leukocytes, and because both ...200919356397
intra-abdominal pressure development after different temporary abdominal closure techniques in a porcine model.decompressive laparotomy followed by temporary abdominal closure (tac) is an established prophylaxis and treatment for abdominal compartment syndrome. the herein presented study aimed at the comparison of volume reserve capacity and development of intra-abdominal hypertension after forced primary abdominal closure and different tac techniques in a porcine model.200919359923
jejunal pouch reconstruction but not preservation of duodenal passage after total gastrectomy reduces plasma cholecystokinin and pancreatic polypeptide long term in pigs.the long-term effects of reconstructions of the gastrointestinal tract after gastrectomy on plasma levels of gastrointestinal hormones that contribute to food intake controls were evaluated.200919368131
total gastrectomy causes a sustained, long-term elevation of somatostatin in plasma, independent of the mode of reconstruction in pigs.the long-term effects of gastrectomy and various reconstructions of the gastrointestinal tract on fasting plasma levels of gastrointestinal hormones known to contribute to the control of gastrointestinal motor function were evaluated in pigs.200919368132
multipolar radiofrequency ablation in controlling hemorrhage from blunt liver trauma.the aim of this study is to investigate the use of multipolar radiofrequency (rf) ablation with internally cooled electrodes to control hemorrhage percutaneously from blunt liver trauma.200919371528
video-assisted lung resection using radiofrequency ablation in a porcine model.the aim of this study was to assess the feasibility, efficacy, and safety of video-assisted lingula resection using radiofrequency ablation (rfa) with internally cooled electrodes in a porcine model.200919376530
distribution of cocaine- and amphetamine-regulated transcript in the hippocampal formation of the guinea pig and domestic pig.this study provides a detailed description concerning the distribution of cocaineand amphetamine-regulated transcript (cart) subunits - cart(61-102) and rhcart(28-116) - in the hippocampal formation (hf) of the guinea pig and domestic pig, focussing on the dentate gyrus (dg) and hippocampus proper (hp). although in both studied species cart-immunoreactive (cart-ir) neuronal somata and processes were present generally in the same layers, some species-specific differences were still found. in the ...200919384826
[(18)f]fluoroacetate is not a functional analogue of [(11)c]acetate in normal physiology.[(11)c]acetate (c-ac) is a general pet tracer of cellular carbon flux and useful for clinical imaging in heart disease as well as prostate cancer and other tumours. c-ac has a high (70%) whole-body extraction fraction, proportional to blood flow in many organs. trapping is related to organ-specific enzymatic activation and formation of [(11)c]-acetyl-coa, the fate of which has been well characterized. due to the logistic challenges with c-ac, 2-[(18)f]fluoroacetate (f-ac) has been proposed as a ...200919387639
effect of recipient breed on delivery rate of cloned miniature pig.the miniature pig is regarded as a better organ donor breed for xenotransplantation than other pig breeds because the size of their organs is similar to that of humans. to improve efficiency of cloned miniature pig production, we analysed the effect of breed difference between donor cells and embryo recipients on pregnancy rate and delivery rate. cloned porcine embryos derived from domestic or miniature pig donor cells were transferred to domestic or miniature recipient pigs. delivery rate was s ...200919393119
occurrence and pathways of manure-borne 17beta-estradiol in vadose zone water.the hormone 17beta-estradiol (e2) can cause endocrine disruption in sensitive species at part per trillion concentrations. the persistence and transport pathways of manure-borne e2 in agricultural soils were determined by comparing its occurrence with the transfer of water and the transport of non-sorbing fluorobenzoic acid (fba) tracers. this comparison was done using capillary wick lysimeters installed 0.61m beneath three corn (zea mays l.) plots that receive swine (sus scrofa domesticus) manu ...200919394677
feral swine contact with domestic swine: a serologic survey and assessment of potential for disease transmission.feral swine (sus scrofa) are present in 38 of the 50 united states, and their populations continue to expand. domestic swine are widely regarded as vulnerable to diseases harbored by feral swine. our objectives were to determine antibody prevalence for selected pathogens in texas feral swine populations and identify contact events between feral and domestic swine. overall prevalence of antibodies against brucellosis and pseudorabies virus was 11% and 30%, respectively. antibodies to porcine repr ...200919395751
influence of sex on the meiotic segregation of a t(13;17) robertsonian translocation: a case study in the pig.comparison of male versus female meiotic segregation patterns for robertsonian translocation (rt) carriers with similar genetic background has rarely been reported in mammalian species.200919403564
porcine hypothalamic aromatase cytochrome p450: isoform characterization, sex-dependent activity, regional expression, and regulation by enzyme inhibition in neonatal boars.domestic pigs have three cyp19 genes encoding functional paralogues of the enzyme aromatase cytochrome p450 (p450arom) that are expressed in the gonads, placenta, and preimplantation blastocyst. all catalyze estrogen synthesis, but the gonadal-type enzyme is unique in also synthesizing a nonaromatizable biopotent testosterone metabolite, 1oh-testosterone (1oh-t). p450arom is expressed in the vertebrate brain, is higher in males than females, but has not been investigated in pigs, to our knowledg ...200919403926
pre-natal stress amplifies the immediate behavioural responses to acute pain in piglets.pre-natal stress (pns) or undernutrition can have numerous effects on an individual's biology throughout their lifetime. some of these effects may be adaptive by allowing individuals to tailor their phenotype to environmental conditions. here we investigated, in the domestic pig sus scrofa, whether one possible consequence of a predicted adverse environment could be altered pain perception. the behavioural response of piglets to the surgical amputation ('docking') of their tail or a sham procedu ...200919411272
relaxin and maternal lactocrine programming of neonatal uterine development.in mammals, including the pig (sus scrofa domesticus), structural patterning and functional programming of uterine tissues involve events that occur shortly after birth. porcine endometrial development between birth (postnatal day 0 [pnd 0]) and pnd 15 is estrogen receptor (er) dependent and estrogen sensitive. the endometrium is relaxin (rlx) receptor (rxfp1) positive and eralpha negative at birth. uterine expression of rxfp1 and eralpha, detectable by pnd 2, increases with age from pnd 0 to 14 ...200919416179
recombinant antigen targets for serodiagnosis of african swine fever.african swine fever (asf) is an infectious and economically important disease of domestic pigs. there is no vaccine, and so reliable diagnosis is essential for control strategies. the performance of four recombinant asf virus (asfv) protein (pk205r, pb602l, p104r, and p54)-based enzyme-linked immunosorbent assays (elisas) was evaluated with european porcine field sera that had been established by office international des epizooties (oie)-approved tests to be asfv negative (n = 119) and asfv posi ...200919420186
cadaveric volatile organic compounds released by decaying pig carcasses (sus domesticus l.) in different biotopes.forensic entomology uses pig carcasses to surrogate human decomposition and to investigate the entomofaunal colonization. insects communicate with their environment through the use of chemical mediators, which in the case of necrophagous insects, may consist in the cadaveric volatile organic compounds (vocs) released by the corpse under decomposition. previous studies have focused on cadaveric vocs released from human corpses. nevertheless, studies on human corpses are restricted for many reason ...200919423246
pure 'natural orifice transluminal endoscopic surgery' for transvaginal nephrectomy in the porcine model.to determine the technical feasibility and reproducibility of pure natural orifice transluminal endoscopic surgery (notes) transvaginal nephrectomy using notes-specific instrumentation, with no transabdominal assistance.200919426194
an integrated rh map of porcine chromosome 10.whole genome radiation hybrid (wg-rh) maps serve as "scaffolds" to significantly improve the orientation of small bacterial artificial chromosome (bac) contigs, order genes within the contigs and assist assembly of a sequence-ready map for virtually any species. here, we report the construction of a porcine: human comparative map for pig (sus scrofa) chromosome 10 (ssc10) using the imnprh2(12,000-rad) porcine wg-rh panel, integrated with the imprh(7000-rad) wg-rh, genetic and bac fingerprinted c ...200919426492
detection of hepatitis e virus in wild boars of rural and urban regions in germany and whole genome characterization of an endemic strain.hepatitis e is an increasingly diagnosed human disease in central europe. besides domestic pigs, in which hepatitis e virus (hev) infection is highly prevalent, wild boars have been identified as a possible source of human infection. in order to assess the distribution of hev in the wild boar population of germany, we tested liver samples originating from different geographical regions for the presence of the hev genome and compared the detected sequences to animal and human hev strains.200919442307
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