Publications

socioeconomic and ecological factors influencing aedes aegypti prevalence, abundance, and distribution in dhaka, bangladesh.	this study examined household risk factors and prevalence, abundance, and distribution of immature aedes aegypti and aedes albopictus, and their association with socioeconomic and ecological factors at urban zonal and household levels in the city of dhaka, bangladesh. during the 2011 monsoon, 826 households in 12 randomly selected administrative wards were surveyed for vector mosquitoes. results revealed that the abundance and distribution of immature ae. aegypti and ae. albopictus, and pupae-pe ...	2016	27022149
control of urban zika vectors: should we return to the successful paho/who strategy?		2016	27249063
the mouthparts enriched odorant binding protein 11 of the alfalfa plant bug adelphocoris lineolatus displays a preferential binding behavior to host plant secondary metabolites.	odorant binding proteins (obps) are proposed to be directly required for odorant discrimination and represent potential interesting targets for pest control. in the notoriously agricultural pest adelphocoris lineolatus, our previous functional investigation of highly expressed antennal obps clearly supported this viewpoint, whereas the findings of the current study by characterizing of alinobp11 rather indicated that obp in hemipterous plant bugs might fulfill a different and tantalizing physiol ...	2016	27313540
another pandemic disaster looms: yellow fever spreading from angola.		2016	27642446
combinatorial gene regulatory functions underlie ultraconserved elements in drosophila.	ultraconserved elements (uces) are discrete genomic elements conserved across large evolutionary distances. although uces have been linked to multiple facets of mammalian gene regulation their extreme evolutionary conservation remains largely unexplained. here, we apply a computational approach to investigate this question in drosophila, exploring the molecular functions of more than 1,500 uces shared across the genomes of 12 drosophila species. our data indicate that drosophila uces are hubs fo ...	2016	27247329
antifilarial activity of diterpenoids from taxodium distichum.	lymphatic filariasis caused by wuchereria bancrofti, brugia malayi and b. timori, is a debilitating disease with an adverse social and economic impact. the infection remains unabated in spite of treatment with existing antifilarial drugs diethylcarbamazine (dec) and ivermectin which are chiefly microfilaricides. there is therefore, need for macrofilaricides, embryostatic agents and better microfilaricides. in the present study we explored the antifilarial potential of crude extract and its molec ...	2016	27245322
outwitting dengue threat and epidemics resurgence in asia-pacific countries: strengthening integrated dengue surveillance, monitoring and response systems.	dengue is still a substantial vector-borne viral disease threat and burden of public health importance worldwide. this situation is complicated by dengue virus unprecedented resurgence and persistence of varied serotypes in endemic-prone areas, and man-made and natural activities consequences that promote vector emergence, transmission dynamics and spread across the asia-pacific region. there is an urgent need to strengthen operational and contextual surveillance-response research in improving e ...	2016	27233238
correction: spread of the invasive mosquitoes aedes aegypti and aedes albopictus in the black sea region increases risk of chikungunya, dengue, and zika outbreaks in europe.	[this corrects the article doi: 10.1371/journal.pntd.0004664.].	2016	27232603
different effects of metarhizium anisopliae strains imi330189 and ibc200614 on enzymes activities and hemocytes of locusta migratoria l.	metarhizium is an important class of entomopathogenic fungi in the biocontrol of insects, but its virulence is affected by insect immunity. to clarify the mechanism in virulence of metarhizium, we compared the immunological differences in locusta migratoria l. when exposed to two strains of metarhizium anisopliae (ma).	2016	27227835
experimental infection of rhodnius prolixus (hemiptera, triatominae) with mycobacterium leprae indicates potential for leprosy transmission.	leprosy is a chronic dermato-neurological disease caused by infection with mycobacterium leprae. in 2013 almost 200,000 new cases of leprosy were detected around the world. since the first symptoms take from years to decades to appear, the total number of asymptomatic patients is impossible to predict. although leprosy is one of the oldest records of human disease, the mechanisms involved with its transmission and epidemiology are still not completely understood. in the present work, we experime ...	2016	27203082
exposure to aedes aegypti bites induces a mixed-type allergic response following salivary antigens challenge in mice.	classical studies have shown that aedes aegypti salivary secretion is responsible for the sensitization to mosquito bites and many of the components present in saliva are immunogenic and capable of inducing an intense immune response. therefore, we have characterized a murine model of adjuvant-free systemic allergy induced by natural exposure to mosquito bites. balb/c mice were sensitized by exposure to a. aegypti mosquito bites and intranasally challenged with phosphate-buffered saline only or ...	2016	27203689
molecular characterization and function analysis of the vitellogenin receptor from the cotton bollworm, helicoverpa armigera (hübner) (lepidoptera, noctuidae).	developing oocytes accumulate plentiful yolk protein during oogenesis through receptor-mediated endocytosis. the vitellogenin receptor (vgr), belonging to the low-density lipoprotein receptor (ldlr) family, regulates the absorption of yolk protein. in this work, the full-length vitellogenin receptor (havgr) in the cotton bollworm helicoverpa armigera was identified, encoding a 1817 residue protein. sequence alignment revealed that the sequence of havgr contained all of the conservative structura ...	2016	27192057
mammalian african trypanosome vsg coat enhances tsetse's vector competence.	tsetse flies are biological vectors of african trypanosomes, the protozoan parasites responsible for causing human and animal trypanosomiases across sub-saharan africa. currently, no vaccines are available for disease prevention due to antigenic variation of the variant surface glycoproteins (vsg) that coat parasites while they reside within mammalian hosts. as a result, interference with parasite development in the tsetse vector is being explored to reduce disease transmission. a major bottlene ...	2016	27185908
a critical role for the drosophila dopamine d1-like receptor dop1r2 at the onset of metamorphosis.	insect metamorphosis relies on temporal and spatial cues that are precisely controlled. previous studies in drosophila have shown that untimely activation of genes that are essential to metamorphosis results in growth defects, developmental delay and death. multiple factors exist that safeguard these genes against dysregulated expression. the list of identified negative regulators that play such a role in drosophila development continues to expand.	2016	27184815
an rna-dependent rna polymerase gene in bat genomes derived from an ancient negative-strand rna virus.	endogenous bornavirus-like l (ebll) elements are inheritable sequences derived from ancient bornavirus l genes that encode a viral rna-dependent rna polymerase (rdrp) in many eukaryotic genomes. here, we demonstrate that bats of the genus eptesicus have preserved for more than 11.8 million years an ebll element named eebll-1, which has an intact open reading frame of 1,718 codons. the eebll-1 coding sequence revealed that functional motifs essential for mononegaviral rdrp activity are well conse ...	2016	27174689
de novo transcriptome analysis and detection of antimicrobial peptides of the american cockroach periplaneta americana (linnaeus).	cockroaches are surrogate hosts for microbes that cause many human diseases. in spite of their generally destructive nature, cockroaches have recently been found to harbor potentially beneficial and medically useful substances such as drugs and allergens. however, genomic information for the american cockroach (periplaneta americana) is currently unavailable; therefore, transcriptome and gene expression profiling is needed as an important resource to better understand the fundamental biological ...	2016	27167617
comprehensive evaluation of candidate reference genes for qrt-pcr studies of gene expression in mustard aphid, lipaphis erysimi (kalt).	mustard aphid, also known as turnip aphid (lipaphis erysimi) is a major insect pest of rapeseed-mustard group of crops. tremendous economic significance has led to substantial basic research involving gene-expression studies in this insect species. in qrt-pcr analysis of gene-expression, normalization of data against rna variation by using appropriate reference gene is fundamental. however, appropriate reference genes are not known in case of l. erysimi. we evaluated 11 candidate reference genes ...	2016	27165720
myosin myth4-ferm structures highlight important principles of convergent evolution.	myosins containing myth4-ferm (myosin tail homology 4-band 4.1, ezrin, radixin, moesin, or mf) domains in their tails are found in a wide range of phylogenetically divergent organisms, such as humans and the social amoeba dictyostelium (dd). interestingly, evolutionarily distant mf myosins have similar roles in the extension of actin-filled membrane protrusions such as filopodia and bind to microtubules (mt), suggesting that the core functions of these mf myosins have been highly conserved over ...	2016	27166421
midgut transcriptome of the cockroach periplaneta americana and its microbiota: digestion, detoxification and oxidative stress response.	the cockroach, periplaneta americana, is an obnoxious and notorious pest of the world, with a strong ability to adapt to a variety of complex environments. however, the molecular mechanism of this adaptability is mostly unknown. in this study, the genes and microbiota composition associated with the adaptation mechanism were studied by analyzing the transcriptome and 16s rdna pyrosequencing of the p. americana midgut, respectively. midgut transcriptome analysis identified 82,905 unigenes, among ...	2016	27153200
hawkmoths evaluate scenting flowers with the tip of their proboscis.	pollination by insects is essential to many ecosystems. previously, we have shown that floral scent is important to mediate pollen transfer between plants (kessler et al., 2015). yet, the mechanisms by which pollinators evaluate volatiles of single flowers remained unclear. here, nicotiana attenuata plants, in which floral volatiles have been genetically silenced and its hawkmoth pollinator, manduca sexta, were used in semi-natural tent and wind-tunnel assays to explore the function of floral sc ...	2016	27146894
evolution, expression, and function of nonneuronal ligand-gated chloride channels in drosophila melanogaster.	ligand-gated chloride channels have established roles in inhibitory neurotransmission in the nervous systems of vertebrates and invertebrates. paradoxically, expression databases in drosophila melanogaster have revealed that three uncharacterized ligand-gated chloride channel subunits, cg7589, cg6927, and cg11340, are highly expressed in nonneuronal tissues. furthermore, subunit copy number varies between insects, with some orders containing one ortholog, whereas other lineages exhibit copy numb ...	2016	27172217
ionotropic chemosensory receptors mediate the taste and smell of polyamines.	the ability to find and consume nutrient-rich diets for successful reproduction and survival is fundamental to animal life. among the nutrients important for all animals are polyamines, a class of pungent smelling compounds required in numerous cellular and organismic processes. polyamine deficiency or excess has detrimental effects on health, cognitive function, reproduction, and lifespan. here, we show that a diet high in polyamine is beneficial and increases reproductive success of flies, and ...	2016	27145030
imbalanced hemolymph lipid levels affect feeding motivation in the two-spotted cricket, gryllus bimaculatus.	insect feeding behavior is regulated by many intrinsic factors, including hemolymph nutrient levels. adipokinetic hormone (akh) is a peptide factor that modulates hemolymph nutrient levels and regulates the nutritional state of insects by triggering the transfer of lipids into the hemolymph. we recently demonstrated that rna interference (rnai)-mediated knockdown of the akh receptor (akhr) reduces hemolymph lipid levels, causing an increase in the feeding frequency of the two-spotted cricket, gr ...	2016	27144650
sequence conservation and sexually dimorphic expression of the ftz-f1 gene in the crustacean daphnia magna.	identifying the genes required for environmental sex determination is important for understanding the evolution of diverse sex determination mechanisms in animals. orthologs of drosophila orphan receptor fushi tarazu factor-1 (ftz-f1) are known to function in genetic sex determination. in contrast, their roles in environmental sex determination remain unknown. in this study, we have cloned and characterized the ftz-f1 ortholog in the branchiopod crustacean daphnia magna, which produces males in ...	2016	27138373
differential gene expression analysis of the epacromius coerulipes (orthoptera: acrididae) transcriptome.	epacromius coerulipes (ivanov) is one of the most widely distributed locusts. to date, the main methods to kill locusts still rely on chemical controls, which can result in the selection of locusts with resistance to chemical pesticides. butene-fipronil is a new pesticide that was discovered by the structural modification of fipronil. this pesticide has been used to control various agricultural pests and has become an important pesticide product to control pests that exhibit resistance to other ...	2016	27142308
serological and entomological study of dengue in dang and chitwan districts of nepal.	a descriptive cross-sectional study was carried out among 264 suspected dengue patients in two districts (dang and chitwan) of nepal from june 2013 to november 2013. the anti-dengue igm positivity was found to be (51/264)19.31% by capture elisa, of which 21 (41.2%) were male and 30 (58.8%) were female. symptoms of seropositive cases were fever, anorexia, nausea, headache, retro-orbital pain, skin rashes, and myalgia. hematological features like thrombocytopenia and leucopenia were found to be si ...	2016	26828951
climate variation drives dengue dynamics.	dengue, a viral infection transmitted between people by mosquitoes, is one of the most rapidly spreading diseases in the world. here, we report the analyses covering 11 y (2005-2015) from the city of guangzhou in southern china. using the first 8 y of data to develop an ecologically based model for the dengue system, we reliably predict the following 3 y of dengue dynamics-years with exceptionally extensive dengue outbreaks. we demonstrate that climate conditions, through the effects of rainfall ...	2016	27940911
resistance to genetic insect control: modelling the effects of space.	genetic insect control, such as self-limiting ridl(2) (release of insects carrying a dominant lethal) technology, is a development of the sterile insect technique which is proposed to suppress wild populations of a number of major agricultural and public health insect pests. this is achieved by mass rearing and releasing male insects that are homozygous for a repressible dominant lethal genetic construct, which causes death in progeny when inherited. the released genetically engineered ('ge') in ...	2016	27816677
molecular virologic and clinical characteristics of a chikungunya fever outbreak in la romana, dominican republic, 2014.	since emerging in saint martin in 2013, chikungunya virus (chikv), an alphavirus transmitted by the aedes aegypti mosquito, has infected approximately two million individuals in the americas, with over 500,000 reported cases in the dominican republic (dr). chikv-infected patients typically present with a febrile syndrome including polyarthritis/polyarthralgia, and a macropapular rash, similar to those infected with dengue and zika viruses, and malaria. nevertheless, many dominican cases are unco ...	2016	28030537
imitedb: the genome-wide landscape of miniature inverted-repeat transposable elements in insects.	miniature inverted-repeat transposable elements (mites) have attracted much attention due to their widespread occurrence and high copy numbers in eukaryotic genomes. however, the systematic knowledge about mites in insects and other animals is still lacking. in this study, we identified 6012 mite families from 98 insect species genomes. comparison of these mites with known mites in the ncbi non-redundant database and repbase showed that 5701(∼95%) of 6012 mite families are novel. the abundance o ...	2016	28025339
an overview of trypanosoma brucei infections: an intense host-parasite interaction.	trypanosoma brucei rhodesiense and t. brucei gambiense, the causative agents of human african trypanosomiasis, are transmitted by tsetse flies. within the vector, the parasite undergoes through transformations that prepares it to infect the human host. sequentially these developmental stages are the replicative procyclic (in which the parasite surface is covered by procyclins) and trypo-epimastigote forms, as well as the non-replicative, infective, metacyclic form that develops in the vector sal ...	2016	28082973
functional evolution of a morphogenetic gradient.	bone morphogenetic proteins (bmps) pattern the dorsal-ventral axis of bilaterian embryos; however, their roles in the evolution of body plan are largely unknown. we examined their functional evolution in fly embryos. bmp signaling specifies two extraembryonic tissues, the serosa and amnion, in basal-branching flies such as megaselia abdita, but only one, the amnioserosa, in drosophila melanogaster. the bmp signaling dynamics are similar in both species until the beginning of gastrulation, when b ...	2016	28005004
control of vector-borne human parasitic diseases.		2016	28090535
spatiotemporal frameworks for infectious disease diffusion and epidemiology.	emerging infectious diseases, and the resurgence of previously controlled infectious disease (e.g., malaria, tuberculosis), are a major focus for public health concern, as well as providing challenges for establishing aetiology and transmission. [...].	2016	27999420
dengue fever-associated maculopathy and panuveitis in australia.	purpose. to describe a case of dengue fever-associated maculopathy and panuveitis to raise awareness of these ophthalmic complications of dengue in australia in the light of recent increasing numbers of outbreaks from equatorial through to tropical australia. case report. a 37-year-old caucasian australian male returning from cambodia presented with a bilateral dengue fever-associated maculopathy with left panuveitis diagnosed clinically and haematologically. automated perimetry revealed bilater ...	2016	28078150
cloning, expression, and characterization of prophenoloxidases from asian corn borer, ostrinia furnacalis (gunée).	insect phenoloxidase (po) belongs to the type 3 copper protein family and possesses oxidoreductase activities. po is typically synthesized as a zymogen called prophenoloxidase (ppo) and requires the proteolytic activation to function. we here cloned full-length cdna for 3 previously unidentified ppos, which we named ofppo1a, ofppo1b, and ofppo3, from asian corn borer, ostrinia furnacalis (gunée), in addition to the previously known ofppo2. these conceptual ppos and ofppo2 all contain two common ...	2016	28078308
data documenting the potential distribution of aedes aegypti in the center of veracruz, mexico.	the data presented in this article are related to the research article entitled "establishment of aedes aegypti (l.) in mountainous regions in mexico: increasing number of population at risk of mosquito-borne disease and future climate conditions" (m. equihua, s. ibáñez-bernal, g. benítez, i. estrada-contreras, c.a. sandoval-ruiz, f.s. mendoza-palmero, 2016) [1]. this article provides presence records in shapefile format used to generate maps of potential distribution of aedes aegypti with diffe ...	2016	28054003
economic report on the cost of dengue fever in vietnam: case of a provincial hospital.	dengue is a mosquito-borne viral illness with the world's fastest rate of infection. in 2014, vietnam had recorded 43,000 cases in 53 provinces, with 28 deaths.	2016	28031723
attempts to image the early inflammatory response during infection with the lymphatic filarial nematode brugia pahangi in a mouse model.	helminth parasites remain a major constraint upon human health and well-being in many parts of the world. treatment of these infections relies upon a very small number of therapeutics, most of which were originally developed for use in animal health. a lack of high throughput screening systems, together with limitations of available animal models, has restricted the development of novel chemotherapeutics. this is particularly so for filarial nematodes, which are long-lived parasites with a compl ...	2016	27992545
rna interference as a gene silencing tool to control tuta absoluta in tomato (solanum lycopersicum).	rna interference (rnai), a gene-silencing mechanism that involves providing double-stranded rna molecules that match a specific target gene sequence, is now widely used in functional genetic studies. the potential application of rnai-mediated control of agricultural insect pests has rapidly become evident. the production of transgenic plants expressing dsrna molecules that target essential insect genes could provide a means of specific gene silencing in larvae that feed on these plants, resultin ...	2016	27994959
variations on a theme: antennal lobe architecture across coleoptera.	beetles comprise about 400,000 described species, nearly one third of all known animal species. the enormous success of the order coleoptera is reflected by a rich diversity of lifestyles, behaviors, morphological, and physiological adaptions. all these evolutionary adaptions that have been driven by a variety of parameters over the last about 300 million years, make the coleoptera an ideal field to study the evolution of the brain on the interface between the basic bauplan of the insect brain a ...	2016	27973569
identification of development-related genes in the ovaries of adult harmonia axyridis (pallas) lady beetles using a time- series analysis by rna-seq.	adults of the lady beetle species harmonia axyridis (pallas) are bred artificially en masse for classic biological control, which requires egg-laying by the h. axyridis ovary. development-related genes may impact the growth of the h. axyridis adult ovary but have not been reported. here, we used integrative time-series rna-seq analysis of the ovary in h. axyridis adults to detect development-related genes. a total of 28,558 unigenes were functionally annotated using seven types of databases to o ...	2016	27966611
how do households respond to unreliable water supplies? a systematic review.	although the millennium development goal (mdg) target for drinking water was met, in many developing countries water supplies are unreliable. this paper reviews how households in developing countries cope with unreliable water supplies, including coping costs, the distribution of coping costs across socio-economic groups, and effectiveness of coping strategies in meeting household water needs. structured searches were conducted in peer-reviewed and grey literature in electronic databases and sea ...	2016	27941695
aphids transform and detoxify the mycotoxin deoxynivalenol via a type ii biotransformation mechanism yet unknown in animals.	biotransformation of mycotoxins in animals comprises phase i and phase ii metabolisation reactions. for the trichothecene deoxynivalenol (don), several phase ii biotransformation reactions have been described resulting in don-glutathiones, don-glucuronides and don-sulfates made by glutathione-s-transferases, uridine-diphosphoglucuronyl transferases and sulfotransferases, respectively. these metabolites can be easily excreted and are less toxic than their free compounds. here, we demonstrate for ...	2016	27929076
consecutive large dengue outbreaks in taiwan in 2014-2015.		2016	27924810
transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the australian crayfish, cherax quadricarinatus.	the australian redclaw crayfish (cherax quadricarinatus) has recently received attention as an emerging candidate for sustainable aquaculture production in australia and worldwide. more importantly, c. quadricarinatus serves as a good model organism for the commercially important group of decapod crustaceans as it is distributed worldwide, easy to maintain in the laboratory and its reproductive cycle has been well documented. in order to better understand the key reproduction and development reg ...	2016	27924858
retrospective analysis of prognostic factors in dengue infected patients with intracranial bleed.	dengue is one of the most common mosquito-transmitted arboviral disease of tropical and a few subtropical areas in the world. it is estimated that approximately 100 million cases occur per year and approximately 2.5 billion people are at risk of developing dengue infection. hemorrhagic complications causing encephalopathy are quite rare but fatal consequences of this deadly disease. this study was conducted to discuss the prognostic factors in the management of intracranial hemorrhage in dengue ...	2016	28031986
control of aedes aegypti breeding: a novel intervention for prevention and control of dengue in an endemic zone of delhi, india.	the study is based on hypothesis that whether continuous entomological surveillance of ae. aegypti and simultaneous appropriate interventions in key containers during non-transmission (december-may) months would have any impact on breeding of aedes and dengue cases during the following transmission months (june-november). the impact of the surveillance and intervention measures undertaken during non-transmission months were assessed by entomological indicators namely container index (ci), house ...	2016	27918577
beyond traditional antimicrobials: a caenorhabditis elegans model for discovery of novel anti-infectives.	the spread of antibiotic resistance amongst bacterial pathogens has led to an urgent need for new antimicrobial compounds with novel modes of action that minimize the potential for drug resistance. to date, the development of new antimicrobial drugs is still lagging far behind the rising demand, partly owing to the absence of an effective screening platform. over the last decade, the nematode caenorhabditis elegans has been incorporated as a whole animal screening platform for antimicrobials. th ...	2016	27994583
susceptibility of phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host.	a pleiotropic response to the calpain inhibitor mdl28170 was detected in the tomato parasite phytomonas serpens. ultrastructural studies revealed that mdl28170 caused mitochondrial swelling, shortening of flagellum and disruption of trans golgi network. this effect was correlated to the inhibition in processing of cruzipain-like molecules, which presented an increase in expression paralleled by decreased proteolytic activity. concomitantly, a calcium-dependent cysteine peptidase was detected in ...	2016	27925020
size, sounds and sex: interactions between body size and harmonic convergence signals determine mating success in aedes aegypti.	several new mosquito control strategies will involve the release of laboratory reared males which will be required to compete with wild males for mates. currently, the determinants of male mating success remain unclear. the presence of convergence between male and female harmonic flight tone frequencies during a mating attempt have been found to increase male mating success in the yellow fever mosquito, aedes aegypti. size has also been implicated as a factor in male mating success. here, we inv ...	2016	27906074
clinical profiles of dengue infection during an outbreak in northern india.	introduction. dengue fever is an arboviral disease, which is transmitted by mosquito vector and presents as varied clinical spectrum of dengue fever (df), dengue hemorrhagic fever (dhf), dengue shock syndrome (dss), and expanded dengue syndrome (eds) with atypical presentations, thus posing a diagnostic dilemma. unless we are aware of these presentations, diagnosis as well as early initiation of treatment becomes difficult. we studied the various clinical presentations of dengue infection during ...	2016	28025597
path of infectious diseases in brazil in the last 50 years: an ongoing challenge.	in this article, we comment on the main features of infectious diseases in brazil in the last 50 years, highlighting how much of this path revista de saúde pública could portray. from 1967 to 2016, 1,335 articles focusing on infectious diseases were published in revista de saúde pública. although the proportion of articles on the topic have decreased from about 50.0% to 15.0%, its notability remained and reflected the growing complexity of the research required for its control. it is noteworthy ...	2016	28099652
contribution of oswaldo paulo forattini to public health: analysis of scientific production.	to analyze the main characteristics of the scientific production of oswaldo paulo forattini, researcher and, for 40 years, editor of revista de saúde pública.	2016	28099651
small rnas from bemisia tabaci are transferred to solanum lycopersicum phloem during feeding.	the phloem-feeding whitefly bemisia tabaci is a serious pest to a broad range of host plants, including many economically important crops such as tomato. these insects serve as a vector for various devastating plant viruses. it is known that whiteflies are capable of manipulating host-defense responses, potentially mediated by effector molecules in the whitefly saliva. we hypothesized that, beside putative effector proteins, small rnas (srna) are delivered by b. tabaci into the phloem, where the ...	2016	27933079
the worldwide spread of the tiger mosquito as revealed by mitogenome haplogroup diversity.	in the last 40 years, the asian tiger mosquito aedes albopictus, indigenous to east asia, has colonized every continent except antarctica. its spread is a major public health concern, given that this species is a competent vector for numerous arboviruses, including those causing dengue, chikungunya, west nile, and the recently emerged zika fever. to acquire more information on the ancestral source(s) of adventive populations and the overall diffusion process from its native range, we analyzed th ...	2016	27933090
amino acids trigger down-regulation of superoxide via torc pathway in the midgut of rhodnius prolixus.	sensing incoming nutrients is an important and critical event for intestinal cells to sustain life of the whole organism. the torc is a major protein complex involved in monitoring the nutritional status and is activated by elevated amino acid concentrations. an important feature of haematophagy is that huge amounts of blood are ingested in a single meal, which results in the release of large quantities of amino acids, together with the haemoglobin prosthetic group, haem, which decomposes hydrop ...	2016	26945025
immunodominant dengue virus-specific cd8+ t cell responses are associated with a memory pd-1+ phenotype.	dengue disease is a large public health problem that mainly afflicts tropical and subtropical regions. understanding of the correlates of protection against dengue virus (denv) is poor and hinders the development of a successful human vaccine. the present study aims to define denv-specific cd8(+)t cell responses in general and those of hla alleles associated with dominant responses in particular. in human blood donors in nicaragua, we observed a striking dominance of hla b-restricted responses i ...	2016	26912627
[hemorrhagic form of dengue fever observed at the infectious diseases department chu yalgado ouédraogo, burkina faso].	dengue is a re-emerging arboviral disease in tropical countries. haemorrhagic form may be associated with circulatory failure and produce hypovolemic shock, which is often fatal. we report a series of three cases of hemorrhagic dengue observed at the infectious diseases department chu yalgado ouédraogo, ouagadougou, in order to describe its epidemiological, clinical, and evolutive characteristics. our study included young females who had an onset age of between 35 years and 45 years, residing in ...	2016	27303584
indole alkaloids of the stigonematales (cyanophyta): chemical diversity, biosynthesis and biological activity.	the cyanobacteria are well recognized as producers of a wide array of bioactive metabolites including toxins, and potential drug candidates. however, a limited number of taxa are generally considered with respect to both of these aspects. that said, the order stigonematales, although largely overlooked in this regard, has become increasingly recognized as a source of bioactive metabolites relevant to both human and environmental health. in particular, the hapalindoles and related indole alkaloid ...	2016	27058546
polyandry depends on postmating time interval in the dengue vector aedes aegypti.	aedes aegypti is the primary vector of the dengue and chikungunya viruses. after mating, male seminal fluid molecules cause females to become unreceptive to a subsequent mating. this response is often assumed to be immediate and complete, but a growing body of evidence suggests that some females do mate more than once. it is unknown how quickly a female becomes unreceptive to a second mating. furthermore, the degree to which she remains monandrous after laying several batches of eggs has not bee ...	2016	26880776
mathematical model of three age-structured transmission dynamics of chikungunya virus.	we developed a new age-structured deterministic model for the transmission dynamics of chikungunya virus. the model is analyzed to gain insights into the qualitative features of its associated equilibria. some of the theoretical and epidemiological findings indicate that the stable disease-free equilibrium is globally asymptotically stable when the associated reproduction number is less than unity. furthermore, the model undergoes, in the presence of disease induced mortality, the phenomenon of ...	2016	27190548
phytochemical composition, antifungal and antioxidant activity of duguetia furfuracea a. st.-hill.	background. duguetia furfuracea is popular plant used in popular medicine. hypothesis/purpose. this claim evaluated the phytochemical composition of the hydroethanolic extract (hedf), fractions of duguetia furfuracea, and antioxidant and antifungal activity. methods. the chemical profile was carried out by hplc-dad. the total phenolic contents and flavonoid components were determined by folin-ciocalteu and aluminium chloride reaction. the antioxidant activity was measured by scavenging of 2,2-di ...	2016	27127550
vaccination of gerbils with bm-103 and bm-ral-2 concurrently or as a fusion protein confers consistent and improved protection against brugia malayi infection.	the brugia malayi bm-103 and bm-ral-2 proteins are orthologous to onchocerca volvulus ov-103 and ov-ral-2, and which were selected as the best candidates for the development of an o. volvulus vaccine. the b. malayi gerbil model was used to confirm the efficacy of these ov vaccine candidates on adult worms and to determine whether their combination is more efficacious.	2016	27045170
efficacy of four commercially available heartworm preventive products against the jyd-34 laboratory strain of dirofilaria immitis.	heartworm disease in dogs can be severe and life threatening. resistance to available heartworm preventives was considered among potential causes of increased reports of failed heartworm prevention in dogs. the objective of the present study was to compare the efficacy of four commercially available heartworm disease preventives against the jyd-34 strain of d. immitis.	2016	27044379
evaluating the effectiveness of localized control strategies to curtail chikungunya.	chikungunya, a re-emerging arbovirus transmitted to humans by aedes aegypti and ae. albopictus mosquitoes, causes debilitating disease characterized by an acute febrile phase and chronic joint pain. chikungunya has recently spread to the island of st. martin and subsequently throughout the americas. the disease is now affecting 42 countries and territories throughout the americas. while chikungunya is mainly a tropical disease, the recent introduction and subsequent spread of ae. albopictus into ...	2016	27045523
climate change and aedes vectors: 21st century projections for dengue transmission in europe.	warming temperatures may increase the geographic spread of vector-borne diseases into temperate areas. although a tropical mosquito-borne viral disease, a dengue outbreak occurred in madeira, portugal, in 2012; the first in europe since 1920s. this outbreak emphasizes the potential for dengue re-emergence in europe given changing climates. we present estimates of dengue epidemic potential using vectorial capacity (vc) based on historic and projected temperature (1901-2099). vc indicates the vect ...	2016	27322480
a novel video-tracking system to quantify the behaviour of nocturnal mosquitoes attacking human hosts in the field.	many vectors of malaria and other infections spend most of their adult life within human homes, the environment where they bloodfeed and rest, and where control has been most successful. yet, knowledge of peri-domestic mosquito behaviour is limited, particularly how mosquitoes find and attack human hosts or how insecticides impact on behaviour. this is partly because technology for tracking mosquitoes in their natural habitats, traditional dwellings in disease-endemic countries, has never been a ...	2016	27075002
first identification of kdr allele f1534s in vgsc gene and its association with resistance to pyrethroid insecticides in aedes albopictus populations from haikou city, hainan island, china.	aedes albopictus is distributed widely in china, as a primary vector of dengue fever and chikungunya fever in south of china. chemical insecticide control is one of the integrated programmes to prevent mosquito-borne diseases. long-term applications of pyrethroids have resulted in the development of resistance in ae. albopictus populations in china. however, the susceptibility of ae. albopictus to pyrethroids in hainan island was unclear. knockdown resistance (kdr), caused by point mutations in ...	2016	27133234
spatial and temporal country-wide survey of temephos resistance in brazilian populations of aedes aegypti.	the organophosphate temephos has been the main insecticide used against larvae of the dengue and yellow fever mosquito (aedes aegypti) in brazil since the mid-1980s. reports of resistance date back to 1995; however, no systematic reports of widespread temephos resistance have occurred to date. as resistance investigation is paramount for strategic decision-making by health officials, our objective here was to investigate the spatial and temporal spread of temephos resistance in ae. aegypti in br ...	2016	27143489
gravid anopheles gambiae sensu stricto avoid ovipositing in bermuda grass hay infusion and it's volatiles in two choice egg-count bioassays.	a number of mosquito species in the culex and aedes genera prefer to lay eggs in bermuda grass (cynodon dactylon) hay infusions compared to water alone. these mosquitoes are attracted to volatile compounds from the hay infusions making the infusions effective baits in gravid traps used for monitoring vectors of arboviral and filarial pathogens. since bermuda grass is abundant and widespread, it is plausible to explore infusions made from it as a potential low cost bait for outdoor monitoring of ...	2016	27177033
widespread rift valley fever emergence in senegal in 2013-2014.	rift valley fever (rvf), which caused epizootics and epidemics among human and livestock populations, occurred in senegal in 2013-2014. a multidisciplinary field investigation was carried out in 3 regions of senegal. we found 11 confirmed human cases of rift valley fever, including severe cases with encephalitis and retinitis, 1 pool of mosquito (aedes ochraceus), and 52 animals tested positive for the disease. symptoms such as encephalitis and macular retinitis were the most severe cases report ...	2016	27704007
evolutionarily conserved odorant receptor function questions ecological context of octenol role in mosquitoes.	olfaction is a key insect adaptation to a wide range of habitats. in the last thirty years, the detection of octenol by blood-feeding insects has been primarily understood in the context of animal host-seeking. the recent discovery of a conserved octenol receptor gene in the strictly nectar-feeding elephant mosquito toxorhynchites amboinensis (taor8) suggests a different biological role. here, we show that taor8 is a functional ortholog of its counterparts in blood-feeding mosquitoes displaying ...	2016	27849027
the neurotranscriptome of the aedes aegypti mosquito.	a complete genome sequence and the advent of genome editing open up non-traditional model organisms to mechanistic genetic studies. the mosquito aedes aegypti is an important vector of infectious diseases such as dengue, chikungunya, and yellow fever and has a large and complex genome, which has slowed annotation efforts. we used comprehensive transcriptomic analysis of adult gene expression to improve the genome annotation and to provide a detailed tissue-specific catalogue of neural gene expre ...	2016	26738925
successful malaria elimination in the ecuador-peru border region: epidemiology and lessons learned.	in recent years, malaria (plasmodium vivax and plasmodium falciparum) has been successfully controlled in the ecuador-peru coastal border region. the aim of this study was to document this control effort and to identify the best practices and lessons learned that are applicable to malaria control and to other vector-borne diseases. a proximal outcome evaluation was conducted of the robust elimination programme in el oro province, ecuador, and the tumbes region, peru. data collection efforts incl ...	2016	27894320
detection of wolbachia in aedes albopictus and their effects on chikungunya virus.	wolbachia-based vector control strategies have been proposed as a means to augment the currently existing measures for controlling dengue and chikungunya vectors. prior to utilizing wolbachia as a novel vector control strategy, it is crucial to understand the wolbachia-mosquito interactions. in this study, field surveys were conducted to screen for the infection status of wolbachia in field-collected aedes albopictus the effects of wolbachia in its native host toward the replication and dissemin ...	2016	27920393
bioinformatic characterization of mosquito viromes within the eastern united states and puerto rico: discovery of novel viruses.	mosquitoes are efficient, militarily relevant vectors of infectious disease pathogens, including many rna viruses. the vast majority of all viruses are thought to be undiscovered. accordingly, recent studies have shown that viruses discovered in insects are very divergent from known pathogens and that many of them lack appropriate reference sequences in the public databases. given that the majority of viruses are likely still undiscovered, environ mental sampling stands to provide much needed re ...	2016	27346944
seasonal differences in density but similar competitive impact of aedes albopictus (skuse) on aedes aegypti (l.) in rio de janeiro, brazil.	previous studies have shown that the negative effects of density of ae. albopictus on ae. aegypti exceed those of ae. aegypti on ae. albopictus for population growth, adult size, survivorship, and developmental rate. this competitive superiority has been invoked to explain the displacement of ae. aegypti by ae. albopictus in the southeastern usa. in brazil, these species coexist in many vegetated suburban and rural areas. we investigated a related, but less-well-studied question: do effects of a ...	2016	27322537
evaluation of the larvicidal efficacy of five indigenous weeds against an indian strain of dengue vector, aedes aegypti l. (diptera: culicidae).	background and objectives. aedes aegypti, dengue fever mosquito, is primarily associated with the transmission of dengue and chikungunya in tropical and subtropical regions of the world. the present investigations were carried out to assess the larvicidal efficiency of five indigenous weeds against ae. aegypti. methods. the 1,000 ppm hexane and ethanol extracts prepared from the leaves and stem of five plants (achyranthes aspera, cassia occidentalis, catharanthus roseus, lantana camara, and xant ...	2016	26941996
horizontally transferred genetic elements in the tsetse fly genome: an alignment-free clustering approach using batch learning self-organising map (blsom).	tsetse flies (glossina spp.) are the primary vectors of trypanosomes, which can cause human and animal african trypanosomiasis in sub-saharan african countries. the objective of this study was to explore the genome of glossina morsitans morsitans for evidence of horizontal gene transfer (hgt) from microorganisms. we employed an alignment-free clustering method, that is, batch learning self-organising map (blsom), in which sequence fragments are clustered based on the similarity of oligonucleotid ...	2016	28074180
phylosymbiosis: relationships and functional effects of microbial communities across host evolutionary history.	phylosymbiosis was recently proposed to describe the eco-evolutionary pattern, whereby the ecological relatedness of host-associated microbial communities parallels the phylogeny of related host species. here, we test the prevalence of phylosymbiosis and its functional significance under highly controlled conditions by characterizing the microbiota of 24 animal species from four different groups (peromyscus deer mice, drosophila flies, mosquitoes, and nasonia wasps), and we reevaluate the phylos ...	2016	27861590
synergistic and compensatory effects of two point mutations conferring target-site resistance to fipronil in the insect gaba receptor rdl.	insecticide resistance can arise from a variety of mechanisms, including changes to the target site, but is often associated with substantial fitness costs to insects. here we describe two resistance-associated target-site mutations that have synergistic and compensatory effects that combine to produce high and persistent levels of resistance to fipronil, an insecticide targeting on γ-aminobytyric acid (gaba) receptors. in nilaparvata lugens, a major pest of rice crops in many parts of asia, we ...	2016	27557781
the p450 cyp6z1 confers carbamate/pyrethroid cross-resistance in a major african malaria vector beside a novel carbamate-insensitive n485i acetylcholinesterase-1 mutation.	carbamates are increasingly used for vector control notably in areas with pyrethroid resistance. however, a cross-resistance between these insecticides in major malaria vectors such as anopheles funestus could severely limit available resistance management options. unfortunately, the molecular basis of such cross-resistance remains uncharacterized in an. funestus, preventing effective resistance management. here, using a genomewide transcription profiling, we revealed that metabolic resistance t ...	2016	27135886
crowdsourcing vector surveillance: using community knowledge and experiences to predict densities and distribution of outdoor-biting mosquitoes in rural tanzania.	lack of reliable techniques for large-scale monitoring of disease-transmitting mosquitoes is a major public health challenge, especially where advanced geo-information systems are not regularly applicable. we tested an innovative crowd-sourcing approach, which relies simply on knowledge and experiences of residents to rapidly predict areas where disease-transmitting mosquitoes are most abundant. guided by community-based resource persons, we mapped boundaries and major physical features in three ...	2016	27253869
silencing of the olfactory co-receptor gene in dendroctonus armandi leads to eag response declining to major host volatiles.	in this study, a polymerase chain reaction (pcr) based on homology genes of orco was utilized to identify darmorco, which is essential for olfaction in d. armandi. the results showed that darmorco shares significant sequence homology with orco proteins had known in other insects. quantitative real-time pcr (qrt-pcr) analysis suggested that darmorco was abundantly expressed in adult d. armandi; by contrast, darmorco showed trace amounts of expression level in other stages. of different tissues, d ...	2016	26979566
contact bioassays with phenoxybenzyl and tetrafluorobenzyl pyrethroids against target-site and metabolic resistant mosquitoes.	mosquito strains that exhibit increased tolerance to the chemical class of compounds with a sodium channel modulator mode of action (pyrethroids and pyrethrins) are typically described as "pyrethroid resistant". resistance to pyrethroids is an increasingly important challenge in the control of mosquito-borne diseases, such as malaria or dengue, because one of the main interventions (the distribution of large numbers of long-lasting insecticide-treated bed nets) currently relies entirely on long- ...	2016	26930058
combining synthetic human odours and low-cost electrocuting grids to attract and kill outdoor-biting mosquitoes: field and semi-field evaluation of an improved mosquito landing box.	on-going malaria transmission is increasingly mediated by outdoor-biting vectors, especially where indoor insecticidal interventions such as long-lasting insecticide treated nets (llins) are widespread. often, the vectors are also physiologically resistant to insecticides, presenting major obstacles for elimination. we tested a combination of electrocuting grids with synthetic odours as an alternative killing mechanism against outdoor-biting mosquitoes.	2016	26789733
the influence of larval competition on brazilian wolbachia-infected aedes aegypti mosquitoes.	with field releases starting in brazil, particular interest must be given to understanding how the endosymbiotic bacterium wolbachia pipientis affects aedes aegypti mosquitoes with a brazilian genetic background. currently, there is limited information on how the bacterium affects phenotypic traits such as larval development rate, metabolic reserves and morphometric parameters in ae. aegypti. here, we analyze for the first time, the effect of wolbachia on these key phenotypes and consider how th ...	2016	27183820
determinants of host feeding success by anopheles farauti.	the proportion of blood meals that mosquitoes take from a host species is a function of the interplay of extrinsic (abundance and location of potential hosts) and intrinsic (innate preference) factors. a mark-release-recapture experiment addressed whether host preference in a population of anopheles farauti was uniform or if there were anthropophilic and zoophilic subpopulations. the corresponding fitness associated with selecting different hosts for blood meals was compared by measuring fecundi ...	2016	26964528
vector-control response in a post-flood disaster setting, honiara, solomon islands, 2014.	the close quartering and exposed living conditions in evacuation centres and the potential increase in vector density after flooding in solomon islands resulted in an increased risk of exposure for the occupants to vectorborne diseases.	2016	27757255
functional and biochemical characterization of three recombinant human glucose-6-phosphate dehydrogenase mutants: zacatecas, vanua-lava and viangchan.	glucose-6-phosphate dehydrogenase (g6pd) deficiency in humans causes severe disease, varying from mostly asymptomatic individuals to patients showing neonatal jaundice, acute hemolysis episodes or chronic nonspherocytic hemolytic anemia. in order to understand the effect of the mutations in g6pd gene function and its relation with g6pd deficiency severity, we report the construction, cloning and expression as well as the detailed kinetic and stability characterization of three purified clinical ...	2016	27213370
male tarsi specific odorant-binding proteins in the diving beetle cybister japonicus sharp.	odorant binding proteins (obps) play critical roles in chemical communication of insects, as they recognize and transport environmental chemical signals to receptors. the diving beetle cybister japonicus sharp shows a remarkable sexual dimorphism. the foreleg tarsi of males are equipped with large suction cups, believed to help holding the female during underwater courtship and mating. here, we identified two obps highly and specifically expressed in male tarsi, suggesting important functions of ...	2016	27545810
the retinoid x receptor from mud crab: new insights into its roles in ovarian development and related signaling pathway.	in arthropods, retinoid x receptor (rxr) is a highly conserved nuclear hormone receptor. by forming a heterodimeric complex with the ecdysone receptor (ecr), rxr is known to be vital importance for various physiological processes. however, in comparison to ecr, the rxr signaling pathway and its roles in crustacean reproduction are poorly understood. in the present study, the rxr mrna was detected in the ovarian follicular cells of mud crab scylla paramamosain (sprxr) and during ovarian maturatio ...	2016	27009370
transcriptomic data from panarthropods shed new light on the evolution of insulator binding proteins in insects : insect insulator proteins.	body plan development in multi-cellular organisms is largely determined by homeotic genes. expression of homeotic genes, in turn, is partially regulated by insulator binding proteins (ibps). while only a few enhancer blocking ibps have been identified in vertebrates, the common fruit fly drosophila melanogaster harbors at least twelve different enhancer blocking ibps. we screened recently compiled insect transcriptomes from the 1kite project and genomic and transcriptomic data from public databa ...	2016	27809783
comparative profiling of micrornas in the winged and wingless english grain aphid, sitobion avenae (f.) (homoptera: aphididae).	micrornas (mirnas) are short single-stranded non-coding rnas that regulate gene expression, particularly during development. in this study, 345 mirnas were identified from the english green aphid, sitobion avenae (f.), of which 168 were conserved and 177 were s. avenae-specific. quantitative comparison of mirna expression levels indicated that 16 and 12 mirnas were significantly up-regulated in winged and wingless s. avenae small rna libraries, respectively. differential expression of these mirn ...	2016	27762301
identification and temporal expression of putative circadian clock transcripts in the amphipod crustacean talitrus saltator.	talitrus saltator is an amphipod crustacean that inhabits the supralittoral zone on sandy beaches in the northeast atlantic and mediterranean. t. saltator exhibits endogenous locomotor activity rhythms and time-compensated sun and moon orientation, both of which necessitate at least one chronometric mechanism. whilst their behaviour is well studied, currently there are no descriptions of the underlying molecular components of a biological clock in this animal, and very few in other crustacean sp ...	2016	27761341
computational characterization of iron metabolism in the tsetse disease vector, glossina morsitans: ire stem-loops.	iron metabolism and regulation is an indispensable part of species survival, most importantly for blood feeding insects. iron regulatory proteins are central regulators of iron homeostasis, whose binding to iron response element (ire) stem-loop structures within the utrs of genes regulate expression at the post-transcriptional level. despite the extensive literature on the mechanism of iron regulation in human, less attention has been given to insect and more specifically the blood feeding insec ...	2016	27503259
gene family evolution reflects adaptation to soil environmental stressors in the genome of the collembolan orchesella cincta.	collembola (springtails) are detritivorous hexapods that inhabit the soil and its litter layer. the ecology of the springtail orchesella cincta is extensively studied in the context of adaptation to anthropogenically disturbed areas. here, we present a draft genome of an o. cincta reference strain with an estimated size of 286.8 mbp, containing 20,249 genes. in total, 446 gene families are expanded and 1,169 gene families evolved specific to this lineage. besides these gene families involved in ...	2016	27289101
composition and genomic organization of arthropod hox clusters.	the ancestral arthropod is believed to have had a clustered arrangement of ten hox genes. within arthropods, hox gene mutations result in transformation of segment identities. despite the fact that variation in segment number/character was common in the diversification of arthropods, few examples of hox gene gains/losses have been correlated with morphological evolution. furthermore, a full appreciation of the variation in the genomic arrangement of hox genes in extant arthropods has not been re ...	2016	27168931
biological screening of selected pacific northwest forest plants using the brine shrimp (artemia salina) toxicity bioassay.	the brine shrimp (artemia salina) bioassay was used to screen 211 methanol extracts from 128 species of pacific northwest plants in search of general cytotoxic activity. strong toxicity (lc50 < 100 µg/ml) was found for 17 extracts from 13 species, with highest activity observed for angelica arguta roots at <10 µg/ml. notably, four species of cedar trees and one of juniper in the family cupressaceae dominated this group with lc50 for heartwood extracts ranging from 15 to 89 µg/ml. moderate toxici ...	2016	27186474