Publications

actions of aspergillus oryzae alpha-amylase, potato phosphorylase, and rabbit muscle phosphorylase a and b on phosphorylated (1----4)-alpha-d-glucan.	aspergillus oryzae alpha-amylase [(1----4)-alpha-d-glucan glucanohydrolase, ec 3.2.1.1] produced o-(6-phosphoryl-alpha-d-glucopyranosyl)-(1----4)-o-alpha-d-glucopyran osy l-(1----4)-d-glucopyranose (6(3)-phosphorylmaltotriose) and o-alpha-d-glucopyranosyl-(1----4)-o-(3-phosphoryl-alpha-d-glucopyranosyl )- (1----4)-o-alpha-d-glucopyranosyl-(1----4)-d-glucopyranose (3(3)-phosphorylmaltotetraose) from potato starch upon exhaustive hydrolysis. these products indicate that the enzyme hydrolyses the s ...	1986	3096568
new alpha-amylase and trypsin inhibitors among the cm-proteins of barley (hordeum vulgare).	barley cm-proteins are a group of at least five salt-soluble components (cma-e) that can be selectively extracted from endosperm with chloroform/methanol mixtures. n-terminal sequences of proteins cma, cmb and cmc have been determined and found to be homologous to those previously determined for cmd and cme, an observation which confirms that their structural genes are members of a dispersed multi-gene family. the purified cm-proteins were tested against trypsin and against alpha-amylases from s ...	1986	3484638
[interrelation of translation regulation and amino acid uptake into cells].		1985	3877528
[protease and alpha-amylase synthesis by washed cells of aspergillus oryzae 251-90].	regularities of protease and alpha-amylase production by washed cells of aspergillus oryzae 251-90 were being studied. the results obtained enabled us to assume a constitutive character of the both enzymes synthesis by the given producer. sources of carbon, nitrogen and sulphur take part in regulation of the protease production, whereas in the case of the alpha-amylase synthesis only carbon sources that are important. elimination of phosphorus from the medium affects the synthesis of both enzyme ...	1985	3885211
nucleotide sequence of the extracellular glucoamylase gene sta1 in the yeast saccharomyces diastaticus.	the complete nucleotide sequence of the extracellular glucoamylase gene sta1 from the yeast saccharomyces diastaticus has been determined. a single open reading frame codes for a 778-amino-acid protein which contains 13 potential n-glycosylation sites. in the 5'- and 3'-flanking regions of the gene, there are striking sequence homologies to the corresponding regions of adh1 for alcohol dehydrogenase and mat alpha 2 for mating type control in the yeast saccharomyces cerevisiae. the putative precu ...	1985	3918017
enzyme replacement with liposomes containing beta-galactosidase from charonia lumpas in murine globoid cell leukodystrophy (twitcher).	enzyme replacement with liposomes containing beta-galactosidase obtained from charonia lumpas was carried out in murine globoid cell leukodystrophy (gld). charonia lumpas beta-galactosidase was able to hydrolyze galactocerebroside trapped into liposomes prepared from lecithin, cholesterol and sulfatide (molar ratio; 7:2:1). liposomes containing charonia lumpas beta-galactosidase were successfully incorporated into the mouse tissues. 3h-galactocerebroside labeled liposomes were also incorporated ...	1985	3919736
[mycoses of the orbit and the eye].		1985	3922126
effect of additive on alfalfa silage fermentation characteristics and feedlot performance of steers.	in each of two trials four large plastic bags were filled with approximately 72 mt of first cutting, early bloom alfalfa (35% dry matter). fresh alfalfa in two bags was treated with .5 kg/metric ton of a commercial silage additive (silo-best). two bags were left untreated. when compared with steers fed untreated alfalfa silage, weight gains, intakes, and feed efficiencies were not significantly (p greater than .05) altered for steers fed treated silage. however, laboratory analysis showed silage ...	1985	3928719
purification and characterization of endo-beta-n-acetylglucosaminidase of aspergillus oryzae.	an endo-beta-n-acetylglucosaminidase which hydrolyzes the n,n'-diacetylchitobiosyl linkage in asparagine-linked oligosaccharides was purified from the enzyme product of aspergillus oryzae. its substrate specificity was similar to that of endo-beta-n-acetylglucosaminidase h from streptomyces griseus with respect to the relative activities toward the glycopeptides obtained from ovalbumin and bovine igg. the present endoglycosidase exhibited a broad optimum ph range and was relatively stable. metal ...	1985	3934149
evidence that the mechanisms of fungitoxicity of 8-quinolinol and its bischelate with copper(ii) are different.	the copper(ii) contents of the growth media, sabouraud dextrose and czapek-dox broths, and of the spore inocula of aspergillus niger (atcc 1004), aspergillus oryzae (atcc 1011), trichoderma viride (atcc 8678), and myrothecium verrucaria (atcc 9095) were determined by atomic absorption spectrophotometry in a graphite furnace. the test systems composed of sabouraud dextrose broth and spore inocula of the four fungi contained only a little over 3% of the copper(ii) required to form a minimal inhibi ...	1985	3935305
the subsite structures of guanine-specific ribonucleases and a guanine-preferential ribonuclease. cleavage of oligoinosinic acids and poly i.	in order to estimate the size of the active site of guanylic acid specific rnases (rnase t1 from aspergillus oryzae and rnase st from streptomyces erythreus) and guanine-preferential rnase (rnase ms from a. saitoi), the depolymerization reaction of oligoinosinic acid, (ip)ni greater than p, having various chain lengths was studied. the kinetic parameters for depolymerization of oligoinosinic acids, (pkm, log v and log v/km) by the three rnases increased with increase of the chain length of the s ...	1985	3936847
a novel enzyme producing isoprimeverose from oligoxyloglucans of aspergillus oryzae.	an enzyme producing isoprimeverose from xyloglucan fragment oligosaccharides has been purified to the electrophoretically pure state from a commercial enzyme preparation of aspergillus oryzae (sanzyme 1000). the purified enzyme showed approximately 1,280-fold increase of the specific activity over the original preparation. the purified enzyme was shown to be an oligomeric protein consisting of two subunits, each of which had a molecular weight of 115,000. the enzyme showed the highest activity a ...	1985	4019436
antifungal properties of 3-n-alkyn-1-ols and synergism with 2-n-alkyn-1-ols and ketoconazole.	twelve 3-n-alkyn-1-ols (c4-c12, c14, c16, and c18) were tested against aspergillus oryzae, aspergillus niger, trichoderma viride, and myrothecium verrucaria in sabouraud dextrose agar at ph 5.6 and 7.0. toxicity to candida albicans, candida tropicalis, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. fungitoxicity was strongly influenced by chain length, slightly by ph of the medium, and significantly ...	1985	4020633
heterokaryosis between aspergillus oryzae cyclopiazonic acid-defective strains: method for estimating the risk of inducing toxin production among cyclopiazonic acid-defective industrial strains.	aspergillus oryzae strains are used extensively in the food industry. some of these strains excrete alpha-cyclopiazonic acid (cpa), a mycotoxin which may provoke toxicoses in rats. physicochemical methods may reveal the presence of this toxin, but they are inadequate to screen cpa-nonproducing (cpa-) strains. cpa production is revealed by either bacterial growth inhibition or alkalinization of the culture medium. this first biological property was used to devise a time-saving screening method to ...	1985	4083874
multiple forms of protyrosinase from aspergillus oryzae and their mode of activation at ph 3.0.	this paper describes the isolation of three molecular forms (i-iii) of protyrosinase and catalytically active tyrosinase ( monophenol ,dihydroxyphenylalanine: oxygen oxidoreductase, e.c. 1.14.18.1) from fresh mycelia of aspergillus oryzae bir 128 by (nh4)2so4 fractionation, successive chromatographies and disc-gel electrophoresis. experimental evidence is presented that purified protyrosinase is contaminated with firmly attached proteinases, and that this contamination may account for both the m ...	1984	6424711
effect of glycolipids contained in liposomes on the incorporation of beta-galactosidase into specific organs.	a series of glycolipids were examined to find a system capable of targeting liposomes into specific organs of rats. sulfatide was found to be the best among the components of liposomes examined for delivering the entrapped enzyme, beta-galactosidase from aspergillus oryzae, into the brain and liver; gangliosides, for the spleen; and sphingomyelin, for the lung. to introduce the enzyme into the liver, galactocerebroside was far better than glucocerebroside. these data suggest that the sugar resid ...	1984	6435635
alteration of the substrate specificity of aspergillus oryzae beta-galactosidase by modification with polyethylene glycol.	beta-galactosidase (beta-d-galactoside galactohydrolase, ec 3.2.1.23) purified from aspergillus oryzae was modified with 2,4,6-trichloro-s-triazine derivatives of polyethylene glycol (activated bpeg) having molecular weights of 600, 1500, 2000, and 4000. polyethylene glycol derivatives were attached to 6 of the 12 amino groups exposed on the surface of the enzyme. upon modification, the enzymatic activity for a water-soluble substrate, o-nitrophenyl beta-d-galactopyranoside, was reduced with inc ...	1984	6436228
[fatty acid composition of the lipids in fungi of the genus aspergillus developing on mineral media with different carbon sources].	this work was aimed at studying the effect of different carbon sources in the composition of mineral media on the growth of fungi belonging to the aspergillus genus and on the fatty acid composition of their lipids. a chemically-defined medium with glucose was shown to be optimal for the growth of 18 aspergillus strains and for the synthesis of lipids by them. the fatty acid composition of lipids was studied when the fungi grew in media with different carbon sources. the lipids were shown to con ...	1984	6442390
[aspergillus oryzae as a cause of keratomycosis in the horse].	a case of a spontaneous mycokeratitis of a previously injured cornea in a horse is described. the infection was caused by aspergillus oryzae. after application of chloramphenicol ophthalmic ointment a corneal clouding was found in the centre which was circularly sharply defined and which - after dispensing dexamethason-neomycin eye drops - expanded all over to a purulent keratitis. the demarcated and initially non purulent mycotic lesions largely improved after the application of tincture of iod ...	1984	6528327
[a case of allergic broncho-pulmonary aspergillosis due to asp. oryzae and its subtype].		1984	6530860
[species and organ heterogeneity of arylsulfatases].		1984	6397752
antifungal properties of 2-n-alkyn-1-ols.	fourteen 2-n-alkynols (c3-c14, c16, and c18) were tested against aspergillus oryzae, aspergillus niger, trichoderma viride, and myrothecium verrucaria in sabouraud dextrose agar at ph 5.6 and 7.0. toxicity to candida albicans, candida tropicalis, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. fungitoxicity was strongly influenced by chain length, slightly by the ph of the medium, and significantly b ...	1984	6098642
the induction of alpha-amylase by starch in aspergillus oryzae: evidence for controlled mrna expression.	the induction of alpha-amylase by starch has been studied in the filamentous fungus aspergillus oryzae. low levels of alpha-amylase activity were found in both intracellular and extracellular samples from glucose-grown cultures. however, alpha-amylase activity increased when starch was the sole carbon source. the intracellular enzyme activity was induced by a factor of approximately 6.5, while the extracellular activity increased 20-fold over that found in the glucose-grown cultures. regardless ...	1984	6208985
secondary structure specificity of the nuclease activity of the 1,10-phenanthroline-copper complex.	the artificial dnase activity of the 1,10-phenanthroline-cuprous ion complex [(op)2cu+] and h2o2 cleaves the a, b, and z forms of dna at different rates. the b structure, formed by most dnas including poly(da-dt) and poly(da) x poly(dt), is the most susceptible to cleavage. it is completely degraded within 1 min by 40 microm 1,10-phenanthroline/4 microm cu2+/7 mm h2o2/7 mm 3-mercaptopropionic acid. the a structure, formed by rna x dna hybrids such as poly(ra) x poly(dt), is cleaved in both stran ...	1984	6320169
endonuclease s1-sensitive site in chicken pro-alpha 2(i) collagen 5' flanking gene region.	a site that is preferentially cleaved by the single-strand-specific endonuclease from aspergillus oryzae was located in vitro 180 base pairs upstream from the 5' end of the chicken pro-alpha 2(i) collagen gene. it is found in supercoiled plasmids with a negative superhelical density of -0.024 or more but not in linear dna molecules. the nuclease s1 sensitivity is retained in plasmids containing genomic fragments extending from position +8 to -285 (where +1 is the first transcribed base) and from ...	1984	6324210
conidium germination rate in wild and domesticated yellow-green aspergilli.	conidia of domesticated yellow-green aspergilli from strains of aspergillus oryzae (ahlburg) cohn and aspergillus sojae sakaguchi and yamada ex murakami, used in the preparation of koji inoculum, germinate approximately 3 h sooner than conidia produced by related wild species, aspergillus flavus link ex fr. and aspergillus parasiticus speare. there was no consistent relationship between average conidium size and estimated 50% germination time. germination trials were conducted on czapek agar at ...	1984	16346471
purification and characterization of extracellular proteinases of aspergillus oryzae.	[this corrects the article on p. 507 in vol. 30.].	1983	16346450
[sedimentation-biochemical method of determination of sedimentation constants and molecular masses of enzymes].	the sedimentation-biochemical method of determination of enzymes' sedimentation coefficients and estimation of their molecular masses based on the tiselius' transport technique is discussed. the method permits to determine the sedimentation coefficients of enzyme in the starting mixtures without their preliminary purification. the use of different substrats permits to carry out the studying of several enzymes simultaneously. the frameworks of the method are discussed.	1983	6353201
[affinity chromatography of aminopeptidases].	the recent data are generalized concerning a series of synthetic oligopeptides which are competitive inhibitors of aminopeptidases of animal, plant and microbic origin. a method for biospecific chromatography of these enzymes is developed, using as ligands such inhibitors as diazo derivatives of p-aminophenyl-, chloromethyl- and methylketones of l-amino acids and peptides, amino acids, aliphatic acid amides. it is established that the most effective inhibitors of aminopeptidases contain l-amino ...	1983	6356542
[isolation of a specific inhibitor of microbial serine proteinase from kidney bean seeds].	a protein acting as a specific inhibitor of microbial serine proteinases was isolated from kidney bean seeds. the purification procedure included complex formation between the inhibitor and aspergillus oryzae proteinase. the protein with a mr approximately 10 000 inhibits subtilisin and asp. oryzae proteinase but does not affect trypsin and chymotrypsin. the inhibitor molecule contains no half-cystine residues.	1983	6357292
effect of drugs on the activity of fungal limit dextrinase & alpha-amylase.		1983	6360862
stereochemical course of dna hydrolysis by nuclease s1.	nuclease s1 hydrolyzes the sp-diastereomer of 5'-o-(2'-deoxyadenosyl)-3'-o-thymidyl phosphorothioate in h2(18)o to [18o]deoxyadenosine 5'-o-phosphorothioate which can be phosphorylated enzymatically to the sp-diastereomer of [alpha-18o]deoxyadenosine 5'-o-(1-thiotriphosphate). 31p nmr spectroscopy shows the oxygen-18 in this compound to be in a nonbridging position at the alpha-phosphorus, indicating that the hydrolysis reaction catalyzed by nuclease s1 proceeds with inversion of configuration a ...	1983	6296110
a chicken repetitive dna sequence that is highly sensitive to single-strand specific endonucleases.	a dna sequence consisting of the 5-mer agagg repeated tandemly 32 times has been detected in a chicken genomic clone and found to be present in about 2000 copies per chicken genome. this sequence was highly susceptible to single-strand specific endonucleases isolated from aspergillus oryzae (s1) and mung bean, but cleavage by a single-strand specific endonuclease isolated from neurospora crassa occurred only at a ph below 5.5. endonucleolytic cutting of the agagg sequence by the single-strand sp ...	1983	6231528
present-day studies on cereals protein nature alpha-amylase inhibitors.		1983	6190085
[effect of the physiological conditions on alpha-amylase and glucoamylase formation by a selected strain of aspergillus oryzae].	the production of alpha-amylase and glucoamylase by a selected strain of aspergillus oryzae was investigated using different carbon and nitrogen sources. the best and most economic fermentation medium for the production of the both amylases in submerged cultures had the following composition (in %): defatted rice brain, 8; corn steep liquor, 3; mgso4 x 7h2o, 0.1; kh2po4, 0.1; cacl2, 0.1. the optimum ph was 5.0. the optimal conditions for biosynthesis of the amylases were as follows: cultivation ...	1983	6413831
effects of thiol protease inhibitors on intracellular degradation of exogenous beta-galactosidase in cultured human skin fibroblasts.	the effects of low molecular weight (lmw) protease inhibitors of microbial origin were evaluated on the intracellular degradation of beta-galactosidase purified from aspergillus oryzae and taken up by cultured human skin fibroblasts with beta-galactosidase deficiency. only thiol protease inhibitors showed an effect to increase the enzyme activity. e-64, a specific inhibitor of thiol proteases, prolonged 3-fold a half life of the exogenous beta-galactosidase and when the enzyme was supplied as li ...	1983	6414834
regulation of the oxidative phase of the pentose phosphate cycle in aspergillus oryzae (ahlburg). i. induction of glucose-6-phosphate dehydrogenase.	the activity of glucose-6-phosphate dehydrogenase changes when the mycelium is grown on different carbon sources. in the presence of ribose, the activity of the enzyme is approximately 3 times higher than when the mycelium is grow in a medium containing glucose. this increase in the enzyme activity is developed progressively when the concentration of ribose increases from 1% to 6% and it is blocked if cycloheximide is added to the medium. this results suggest an increase in "de novo" synthesis o ...	1983	6418104
energy of binding of aspergillus oryzae beta-glucosidase with the substrate, and the mechanism of its enzymic action.	several beta-d-glucopyranosides (p-nitrophenyl, phenyl, and ethyl), 1-thio-beta-d-glucopyranosides, and phenyl 2-deoxy, 3-deoxy, 4-deoxy, and 6-deoxy beta-d-glucopyranosides were synthesized and used to study the mechanism of the enzymatic action of taka-beta-glucosidase [ec 3.2.1.21 aspergillus oryzae]. kinetic constants of the enzyme for these glycosides were determined from s/v-s or 1/v-1/s plots, and the hydrolysis rates of these compounds with the enzyme, acid (3 n hcl) and alkali (3 n naoh ...	1983	6418735
effects of fibrinogen fragment and proteolytic enzyme on the immune system in mice.	the immunomodulating ability of fibrinogen fragment (hol-dsk) and proteolytic enzyme with fibrinolytic activity from aspergillus oryzae was analysed in cba/ca, c57bl, c3h/hej and nmri mice. suppressive effects on the blastogenic response to mitogens were noted. no consistent suppressive effect, but sometimes an enhancing one, was recorded on the antibody responses to protein antigens. administration of hol-dsk or proteolytic enzyme shortly after the immunization resulted in a slight inhibition o ...	1983	6848480
[regulation of alpha-amylase biosynthesis in an aspergillus oryzae 3000 strain based on the principle of feedback from the level of the active enzyme].		1983	6606299
proteinase enzymes relevant to the baking industry.		1982	6754500
degradation of amyloid proteins with protease i from aspergillus oryzae. in vivo increase in saa clearance rate after enzyme infusion.	the effect of the thrombolytic enzyme protease i from aspergillus oryzae (brinase) on the amyloid protein aa was investigated. the effect of the enzyme on purified, low molecular weight protein aa was very high. protein aa in intact amyloid fibrils suspended in neutral buffer was also degraded by the enzyme, although at a much lower rate. amyloid fibrils in tissue sections could also be attacked and removed, leaving the tissue structure fairly intact. the in vivo effect of brinase on protein saa ...	1982	6760382
[a case of allergic bronchopulmonary aspergillosis caused by aspergillus oryzae which is used for brewing bean paste (miso) and soy sauce (shoyu) (author's transl)].		1982	7202073
bilateral endogenous necrotizing scleritis due to aspergillus oryzae.	a case of bilateral necrotizing scleritis due to aspergillus oryzae is reported. the patient was a former addict of intravenous narcotics treated five years previously for meningitis due to the same organism. a seeding focus in the thoracic spine was eventually found. the patient responded well to combined local and systemic therapy with amphotericin b, flucytosine, and natamycin. this represents, to the best of our knowledge, both the first reported case of ocular disease due to this species of ...	1982	6982019
[semicontinuous cultivation of fungi of the genus aspergillus, producers of hydrolases].	the production of exohydrolases (alpha-amylase and pectinase) by fungi belonging to the genus aspergillus was studied in the course of batch cultivation and, if immobilized cells were used, in the semicontinuous regime of growth. the cells were immobilized on a fixed filtering plate and on floating, in the growth medium, polyhedrons. such a cultivation of immobilized microbial cells in the semicontinuous regime of growth on submerged polyhedrons freely floating in the nutrient medium makes it po ...	1982	6984129
effects of phospholipids on substrate specificity of beta-galactosidase purified from aspergillus oryzae.		1982	6807206
a new chromophoric substrate for penicillopepsin and other fungal aspartic proteinases.	the hexapeptide n-alpha-acetylalanylalanyl-lysyl-p- nitrophenylalanylalanylalanylamide has been synthesized and was found to be a good substrate for fungal aspartic proteinases that possess trypsinogen-activating activity, namely penicillopepsin, rhizopus aspartic proteinase, endothia aspartic proteinase and the aspartic proteinases from aspergillus oryzae and penicillium roqueforti. the peptide is rapidly cleaved between the lysine and p-nitrophenylalanine residues. calf chymosin and human reni ...	1982	7052062
liquid-chromatographic determination of the total thiamin content of blood.	a liquid-chromatographic method for determining the total thiamin content of blood is presented. blood is deproteinized and incubated with aspergillus oryzae carboxyl proteinase (ec 3.4.23.6; takadiastase) to convert thiamin phosphate esters to free thiamin. an aliquot of the sample is applied to the column (shodex oh-pak m-414) of a high-performance liquid chromatograph. a 100 mg/l solution of potassium ferricyanide in 150 g/l sodium hydroxide is added to the column effluent with a proportionin ...	1982	7055932
delivery of fungal beta-galactosidase to rat brain by means of liposomes.	a significant increase in beta-galactosidase activity was observed in the brain of rats 1 hr after an intravenous injection of liposomes containing beta-galactosidase purified from aspergillus oryzae. the increased activity was proved to have features of the fungal enzyme by differentiating it from rat's native beta-galactosidase in both heat stability and immunochemical studies. blood content of rat brain tissue under the experimental conditions employed was estimated as 0.83% (v/w) from an inf ...	1982	7071841
purification and characterization of 1,2-alpha-mannosidase of aspergillus oryzae.	1,2-alpha-mannosidase was purified approximately 1,400-fold from an enzyme product of aspergillus oryzae. the enzyme showed a single band in disc gel electrophoresis and the molecular weight was estimated to be about 49,000 daltons by gel exclusion chromatography. the substrate specificity of the enzyme was examined with mannooligosaccharides, yeast mannan, glycopeptides, and a glycoprotein. the alpha-(1 leads to 2)-linking mannose residues located at the nonreducing-ends of the substrates were ...	1982	7118857
[degradation of inactivated alpha-amylase by associated proteases].	alpha-amylase preparations often contain small quantities of proteolytic activity which are difficult to remove. on the example of fungal alpha-amylase, such associated proteases have been shown to possess a specific activity to the denatured amylase molecules. the amylase is not attacked under native conditions, whereas in the thermal denaturation a rapid degradation of only the inactivated molecules occurs. a specific metabolic function of these associated proteases in the return of denatured ...	1982	6183852
kinetic study on chemical modification of taka-amylase a. ii. ethoxycarbonylation of histidine residues.	the modification of taka-amylase a (taa) [ec 3.2.1.1] of aspergillus oryzae by diethylpyrocarbonate (dep) was carried out at 25 degrees c and at ph 5.8 (0.1 m acetate buffer). two out of the six histidine residues were modified with 4.6 mm dep, and two or three histidine residues were modified with 23 mm dep. in both cases, one of them was protected from modification by the presence of 15% maltose. the results suggest that two or three out of the six histidine residues are exposed on the surface ...	1982	6185471
secondary structure of bombyx mori and dictyostelium discoideum 5s rrna from s1 nuclease and cobra venom ribonuclease susceptibility, and computer assisted analysis.	the 5s rrnas from bombyx mori and dictyostelium discoideum were end-labeled with [32-p] at either the 5' or 3' end and sequenced using enzymatic digestion. the secondary structure of these molecules was studied using the single-strand specific s1 nuclease and the base-pair specific cobra venom ribonuclease. computer analysis of these results was performed and was used to generate a consensus secondary structure for each molecule. a comparison of these results with those of other workers is prese ...	1982	6278426
alpha-amylase inhibitor from fungus cladosporium herbarum f-828.	a strain of fungus cladosporium herbarum extracellularly produced an inhibitor specific for mammalian alpha-amylase. the inhibitor was purified 81-fold by freeze-thawing, heat treatment, and column chromatography on deae-cellulose, sephadex g-75, deae-sephacel, and bio-gel p-100. an apparent molecular weight of approximately 18,000 was estimated for the inhibitor using bio-gel p-100 filtration. the purified inhibitor preparation was a glycoprotein containing about 10% carbohydrate. the amino aci ...	1982	6174515
characterization and evaluation of aspergillus oryzae lactase coupled to a regenerable support.	a derivative of crosslinked sepharose, p-(n-acetyl-l-tyrosine azo) benzamidoethyl-cl-sepharose 4b, was synthesized and used for the selective immobilization of thermostable lactase from aspergillus oryzae.preparations of soluble and immobilized lactase were evaluated under initial velocity conditions in a batch process. immobilization had no significant effect on the ph optimum at 50 degrees c or kinetic parameters at ph 4.5 or ph 6.5 and 50 degrees c. at ph 4.5, the soluble enzyme possessed max ...	1982	18546306
confirmation of molecular weight of aspergillus oryzae alpha-amylase using the low angle laser light scattering technique in combination with high pressure silica gel chromatography.	molecular weight of aspergillus oryzae alpha-amylase (taka-amylase a) was estimated to be 51,000 +/- 500 by the combined use of high pressure silica gel (tsk-gel g3000sw) chromatography and the low angle laser light scattering technique. the study was carried out partly to assess the performance of the combined technique, and results obtained indicate that it is highly promising as a method to determined protein molecular weight both accurately and quickly.	1981	6165712
the use of taka-diastase in a[3h]poly(a) hybridization assay of oligo(u) sequences in rna.	a reliable assay of uridylate sequences longer than 10 is described. the procedure is based on the hybridization of [3h]poly(a) with poly(u) or oligo(u) sequences in high ionic conditions and a subsequent degradation of single stranded polynucleotides with purified taka-diastase. a 1:2 complex between poly(a) and poly(u) is formed on which on poly(u) strand is digested by taka-diastase. the procedure is especially suitable for the detection and quantitation of un (n greater than 10) in rna prepa ...	1981	6168676
s1 nuclease of aspergillus oryzae.	s1 nuclease has found many uses in the analysis of the structure of nucleic acids, and more new applications, such as the mapping of splice points of early mrnas in sv40, will undoubtedly be found.	1981	6101052
s1-sensitive sites in dna after gamma-irradiation.	dna from gamma-irradiated t1 bacteriophages was analyzed for "single-stranded" sites by cleavage with s1 nuclease from aspergillus oryzae as lesion probe. the ratio of "s1-sensitive sites" to the amount of radiation-induced single-strand breaks was about one. presumably these "denatured" sites were associated with single-strand breaks. the subsequent check for the persistence of "single-stranded" sites within the dna molecule by thermokinetics demonstrated a strong affinity of the nuclease to it ...	1981	6260190
[isolation and characteristics of highly purified s1-nuclease from amylorizin p10x].	s1-nuclease was purified from the soviet commercial enzyme amylorizin p10x prepared from the surface culture of aspergillus oryzae. the enzyme yield was 33% of total activity. the molecular weight of the enzyme measured by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate was equal to 30,000. the enzyme showed high specificity to single-stranded dna.	1981	6262745
s1 nuclease of aspergillus oryzae: characterization of the associated phosphomonoesterase activity.		1981	6272646
biosynthesis of xyloglucan in suspension-cultured soybean cells. an assay method for xyloglucan xylosyltransferase and attempted synthesis of xyloglucan from udp-d-xylose.	hydrolysis of the total non-cellulosic 14c-polysaccharides from soybean cell wall with carbohydrate hydrolases of an aspergillus oryzae enzyme preparation yielded 14c-monosaccharides and [14c]isoprimeverose (6-o-alpha-d-xylopyranosyl-d-glucopyranose) in which all of the xylosyl residues of the xyloglucan were recovered. based on this finding, an assay method for the activity of xyloglucan xylosyltransferase was developed. when udp-[14c]xylose was incubated with a particulate enzyme fraction from ...	1981	7194336
dna supercoiling, shortening, and induction of single-strand regions by cis-diamminedichloroplatinum(ii).	cis-diamminedichloroplatinum(ii) was found to bind to covalently closed circular supercoiled, covalently closed circular nonsupercoiled, and single-strand broken relaxed pm2 dna and induce different types of dna conformational changes. using kleinschmidt's technique, it was found that binding of cis-diamminedichloroplatinum(ii) decreased the dna length to 75% of the original single-strand broken relaxed dna without inducing superhelical conformational changes. cis-diamminedichloroplatinum(ii) al ...	1981	7197192
[effect of electromagnetic oscillations in the millimeter wave-length range of biological systems].		1981	7017802
[hydrolysis of n-acetyl-l- and n-acetyl-d,l-methionine by microbial aminacylase].	the kinetics of n-acetyl-l- and n-acetyl-d,l-methionine hydrolysis by aminoacylase from asp. oryzae were studied. the maximal rate of the reaction was found to be about 2000 mu moles of l-methionine per mg of protein per hour; km was equal to about 1.10(-1) m for both the racemic and optically active substrates. in the presence of co(ii) ions (the molar ratio of n-acetyl-l-methionine/co(ii) was 100:1) the reaction velocity was increased. the values of approximately 4500 and approximately 3000 mu ...	1981	7284482
[6-phosphogluconate dehydrogenase of aspergillus oryzae (ahlburg). ii. separation and study of isoenzymes].	through ammonium sulphate fractionation followed by ion-exchange chromatography, two forms of 6-phosphogluconate dehydrogenase (6j-phosphogluconate: nadp+ oxidoreductase e.c. 1.1.1.44) were isolated from mycelium of aspergillus oryzae. the km values for 6pg were the same, but the km for nadp+ was smaller for isoenzyme i. the vmx values were also different, with greater activity for isoenzyme ii. the optimum ph for isoenzyme i was 7.5. whereas the isoenzyme ii had two maximum peaks, at ph 7.5 and ...	1981	7339739
a sensitive radiochemical enzyme assay for s-adenosyl-l-homocysteine and l-homocysteine.		1981	6947702
reversal of fungitoxicity of 8-quinolinols and their copper(ii) bischelates. ii. reversal of the action of 8-quinolinol by dl-alpha-lipoic acid.	the effect of amino acids and derivatives, krebs cycle acids and related compounds, fatty acids, and vitamins and related compounds on the toxicity of 8-quinolinol and bis(8-quinolinolato)copper(ii) to aspergillus oryzae (atcc 1011) was studied. only aliphatic thiol-containing compounds (cysteine, glutathione, dithioerythritol, and dithiothreitol) and dl-alpha-lipoic acid protected against 8-quinolinol but not its copper(ii) bischelate. it is suggested that 8-quinolinol inhibits lipoic acid bios ...	1981	6790147
mutagenesis during transformation of bacillus subtilis. i. an increase in "selfing' resulting from hybrid donor dnas.	reverse mutations increase when competent bacillus subtilis cells are transformed with high concentrations of homologous "selfer' dna. a high proportion of the mutants were also transformants of linked genes. a stimulation in the appearance of reversed mutations occurred when homoduplex and heteroduplex "selfer' dnas were used as donors. digestions of native and hybrid dnas with nuclease s1 from aspergillus oryzae resulted in the preferential decrease of mutations as compared to a much smaller i ...	1981	6799809
aminoacylase from aspergillus oryzae. comparison with the pig kidney enzyme.	aminoacylase (ec 3.5.1.14) from aspergillus oryzae was purified from a commercially available crude material by heat treatment, precipitation by polyethyleneimine and ammoniumsulfate, gel chromatography and preparative disc-gel-electrophoresis. the purified product was homogenous as judged by polyacrylamide gel electrophoresis. sds-gel electrophoresis, polyacrylamide-gel-gradient electrohoresis, gel chromatography and amino acid analysis demonstrated the enzyme to be composed of two subunits wit ...	1980	6774495
influence of brinase on fibrinogen: fibrin transition in in vitro and in vivo studies. i. influence of in vitro addition of brinase to plasma on the ethanol gelation test.	the incidence of positive ethanol gelation test (egt) after addition of brinase (a proteolytic enzyme preparation from aspergillus oryzae) to anticoagulated and non-anticoagulated human plasma was studied. in vitro addition of brinase to plasma causes positive egt, and the incidence is dose-dependent. in plasma from warfarin-treated patients and/or after addition of heparin to plasma, prior to the addition of brinase, a significantly reduced incidence of positive egt is observed. the incidence i ...	1980	7351312
[effect of sh-reagents and thiol compounds on aminopeptidase from aspergillus oryzae].	it is shown that iodacetate and iodacetamide produce an insignificant inhibition of the asp. oryzae aminopeptidase activity, para-chloromercuribenzoate is a stronger inhibitor. dithiotreitol, beta-mercaptoethanol, reduced glutathione also cause a considerable loss in the enzyme activity. the inhibitory effect is intensified with a combined action of para-chloromercuribenzoate and edta. the studies of para-chloromercuribenzoate, iodacetate and iodacetamide effect on the activation of aminopeptida ...	1980	7376280
[physicochemical and enzymatic properties of aspergillus oryzae aminopeptidase].	the paper deals with properties of aspergillus oryzae (strain kc) purified aminopeptidase. the enzyme is homogeneous in electrophoresis in polyacrylamide gel and enzyme-electrophoresis with the synthetic substrate leucyl-beta-naphthylamide applied. the molecular mass is 60000-61000 daltons. the amino acidic composition of the enzyme is characterized by a high content of dicarboxylic acids. the substrate specificity is studied. leucyl-glycyl-glycin and leucinamide are most intensive in splitting. ...	1980	7385381
[influence of the sporulation on glucose-6-phosphate dehydrogenase activity from aspergillus oryzae (ahlburg) (author's transl)].	both the specific and the total activity of glucose-6-phosphate dehydrogenase (e.c. 1.1.1.49) of mycelium of aspergillus oryzae decline progressively from day 6th of incubation, when the period of sporulation begins. total cellular protein decreases parallely. cicloheximide stops protein synthesis but not the inactivation of glucose-6-phosphate dehydrogenase. inhibitors of proteolytic enzymes do not prevent the loss of enzyme activity when added to the cultures. experiments show no inhibitors of ...	1980	7403645
homology between prokaryotic and eukaryotic ribonucleases.	there is homology between the amino acid sequences of the extracellular ribonucleases t1 and st, from the eukaryote aspergillus oryzae and the prokaryote streptomyces erythreus, respectively. together with other extracellular ribonucleases homologous to each, these enzymes make up a family of interest to evolutionary biology and useful in studies of protein structure and function.	1980	7411658
[isoenzymes of glucose-6-phosphate dehydrogenase from aspergillus oryzae (ahlburg) (author's transl)].	two forms (i and ii) of glucose-6-phosphate dehydrogenase (d-glucose-6-phosphate: nadp+ oxidoreductase e.c. 1.1.1.49) were isolated from mycelium of aspergillus oryzae grown on glucose as sole carbon source, through ammonium sulfate fractionation, followed by ion-exchange chromatograhy. the km values for both g6p and nadp+ were very similar, but the vmax was nearly twofold for form ii. the two isoenzymes were inhibited by nadph competitively with nadh+, and the ki value was minimal for isoenzyma ...	1980	7221161
[localization of secreted enzyme-induced repression of its synthesis by the cells].	it was shown that under conditions of regulation of secreted enzyme synthesis by its concentration in microorganisms, the repression of synthesis of the enzyme protein polypeptide chain and the concomitant partial repression of labelled amino acid transport into the cells occur. the regulatory effect can be exerted in the absence of transcription as well. these effects are not realized at the post-transcription or post-translation levels.	1980	7248344
intracellular localization of two molecular forms of membrane acid protease in aspergillus oryzae.	the intracellular localization of two forms of membrane-bound acid protease (m1 and m2) [ec 3.4.23.6] of aspergillus oryzae (tsujita, y. & endo, a. (1978) eur. j. biochem. 84, 347-353) was investigated. when the mycelia were treated with wall-lytic enzymes, m2 remained in the cells but most of m2 was solubilized and released. the cell wall fraction obtained by mechanical disruption of the mycelia contained less than 5% of the total acid protease activity in the cells. subcellular fractionation o ...	1980	6997281
molecular mechanisms involved in the production of chromosomal aberrations. ii. utilization of neurospora endonuclease for the study of aberration production by x-rays in g1 and g2 stages of the cell cycle.	chinese hamster ovary cells (cho) were x-irradiated in g1 and g2 stages of the cell cycle and subsequently neurospora endonuclease (ne) (e.c.3.1.4), an enzyme which is specific in cleaving single-stranded dna, was introduced into the cells, after making the cells permeable by treatment with inactivated sendai virus. with this treatment all classes of x-ray-induced chromatid aberrations increased in g2 cells, whereas in g1 cells an increase in chromosome type of aberrations was found, associated ...	1980	6244487
purification and properties of s1 nuclease from aspergillus.		1980	6246349
crystallization of a complex between ribonuclease t1 and 2'-guanylic acid.	ribonuclease t1 was crystallized under various conditions. form i crystals were produced by microdialysis against 53% (v/v) 2-methyl-2,4-pentanediol in 0.01 m sodium acetate, 0.05% 2'-guanylic acid (2'gmp) and 0.02% nan3 (ph 6.2-7.2). these crystals are tetragonal, space group p41212 and contain two molecules per asymmetric unit; cell dimensions are a = b = 5.86 nm, c = 13.28 nm. form iia and form iib crystals were obtained by microdialysis from a buffer of 0.01-0.05 m sodium acetate, 0.25-0.5% ...	1980	6250834
s1 nuclease of aspergillus oryzae: a glycoprotein with an associated nucleotidase activity.		1980	6252849
the inverted repeat as a recognizable structural feature in supercoiled dna molecules.	the single-strand-specific endonuclease s1 from aspergillus oryzae introduces highly selective cleavages into supercoiled covalently closed circular dna molecules, but not into their previously linearized counterparts. the cleavage sites are inverted repeats of unit length between 9 and 13 base pairs, separated by a nonrepetitious 2-6 base pairs. such regions may adopt hairpin or similar structures stabilized by the negative superhelix density and may constitute recognition sites for cellular pr ...	1980	6256738
[properties of the amylolytic enzymes in the drug preparation, orase].		1980	6155292
molecular structure of taka-amylase a. i. backbone chain folding at 3 a resolution.	the crystal structure of taka-amylase a was studied by an x-ray diffraction method at 3 a resolution. a total of 452 amino acid residues were found from the electron density map at the present stage. the four disulfide bonds and the branched carbohydrate were also located on the map. the difference electron density map of the maltotriose-soaked crystal showed that a maltose unit was bound in the active center left. the binding of iodine atoms to the enzyme was also studied.	1980	6156152
[effect of glucose on the induced biosynthesis of alpha-amylase in an aspergillus oryzae 3000 strain].		1980	6176100
isolation and analysis of molds from soy sauce koji in thailand.	five different isolates of aspergillus and one of mucor were compared with a japanese commercial strain of aspergillus oryzae for proteolytic activity on wheat bran substrate. one isolate of aspergillus with superior protease production, identified as aspergillus flavus var. columnaris, showed no detectable aflatoxin production on glutinous rice or soybean substrate. preliminary tests using this fungus as a koji mold in a traditionally operated factory resulted in a soy sauce superior in quality ...	1980	16345516
comparative studies of three exo-beta-glycosidases of aspergillus oryzae.	beta-glucosidase [beta-d-glucoside glucohydrolase ec 3.2.1.21] and beta-galactosidase [beta-d-galactoside galactohydrolase, ec 3.2.1.23] of takadiastase were purified by acetone fractionation, deae-cellulose, and hydroxylapatite chromatography. purity was confirmed by disc electrophoresis, ultracentrifugation and measurement of other glycosidase activities which coexisted in takadiastase. molecular weight of the beta-glucosidase was 218,000 by sedimentation equilibrium and 110,000-116,000 by sds ...	1979	33973
single-stranded regions in streptococcus pneumoniae chromosomal deoxyribonucleic acid and their relation to transformation.	deoxyribonucleic acid (dna) in lysates of both completent and noncompetent streptococcus pneumoniae cells was characterized by chromatography on benzoylated, naphthoylated diethylaminoethyl-cellulose columns, by sensitivity to aspergillus oryzae s1 endonuclease, and by sucrose gradient analysis. the dnas from both competent and noncompetent cells were found to contain similar extents of single-stranded regions. these single-stranded regions appeared to be intact, unpaired regions in double-stran ...	1979	35514
[stability of alpha-amylase with immobilization through its different functional groups].	the paper deals with stability of aspergillus aryzae alpha-amylase immobilized on cm- and ae-celluloses using n,n'-dicyclohexylcarbodiimide by means of binding through its amine or carboxylic groups. the binding of the enzyme with cm-cellulose makes its preparations more stable to the effect of edta and elevated temperature (50 degrees c) than under fixation on ae-cellulose.	1979	36704
[immobilization of aspergillus oryzae aminopeptidase on organic and inorganic carriers].	the process of asp. oryzae aminopeptidase immobilization on organic (ae-cellulose, sepharose 4b, sephadex g-200) and inorganic (sch-2, sch-3 sylochromes and kck n 1 silicagel) carriers was studied. aminopeptidase immobilized on sephadex g-200 contains the largest amount of protein (80 mg per 1 g of carrier) and is the most active of all other preparations. the immobilized preparations retain the temperature optimum, like the soluble form, at 60 degrees c, except the preparation immobilized on ae ...	1979	38548
[properties of amylase immobilized on aerosil derivatives].	the properties of three preparations of alpha-amylase immobilized on aminoaerosil by 2,4-toluylenediisocyanate and n,n'-dicyclohexylcarbodiimide as well as on carboxyaerosil by n,n'-dicyclohexylcarbodiimide were compared with the properties of soluble enzyme. under immobilization the ph-effect and ph-stability zones of amylase are 0.5--1.0 ph units shifted towards the alkaline region. the preparations in which the enzyme is bound with the matrix through the amine groups on carboxyaerosil by n,n' ...	1979	38550
purification of an alkaline nuclease from physarum polycephalum.	an alkaline nuclease was purified from microplasmodia of physarum polycephalum. the nuclease, active on denatured dna and rna and free of contamination by other nucleolytic activities, appeared to be a zinc-metallo protein. the enzyme was only active under conditions, where zn2+ were retained in the enzyme. loss of zinc occurred by the chelating action of edta, egta or ampholines, by acid of highly alkaline ph conditions or by high ionic strength. the addition of zncl2 to compensate losses, rest ...	1979	41584
a note on a novel fungal alkaline proteinase inhibitor from aspergillus oryzae.		1979	42393
[effect of endonuclease s1 on the dna of normal and tumor cells].		1979	42519
low resolution crystal structures of taka-amylase a and its complexes with inhibitors.	the molecular structure of taka-amylase a, an alpha-amylase from aspergillus oryzae, has been studied at 6 a resolution by x-ray diffraction analysis. the electron density map showed a non-crystallographic three-fold screw arrangement of the molecules in the crystal. the molecule is an ellipsoid with approximate dimensions of 80 x 45 x 35 a and contains a hollow which may correspond to the active center. the inhibitor molecules bind to taka-amylase a at four different sites, one of which is loca ...	1979	93603
activation of fungal alpha-amylase by dithioerythritol.	the activity of fungal alpha-amylase has been shown to be influenced by disulfide-reducing reagents. thus, the enzymatic activity increases in the presence of dithioerythritol or 2-mercaptoethanol. l-cysteine is also capable of increasing the activity, but the activation competes with an inactivation reaction which dominates at higher reagent concentrations (greater than 20 mm). a possible scheme interpreting the results is given.	1979	95240
[role of metal ions in the catalytic activity of aspergillus oryzae aminopeptidase].	the paper deals with the role of metals in the catalytic action of asp. oryzae aminopeptidase. cobalt ions are more specific activators than mn2+ and mn2+ and evoke its maximal activity. sinergic activation of co2+ in combination with mn2+ and mg2+ was not found in contrast to some aminopeptidases of animal origin. activation of the enzyme with cobalt chloride depends on temperature. by the 10th minute of incubation the activation reaches its maximum: 650 and 900% at 20 and 40 degrees c, respect ...	1979	106499
aflatoxin produced by five species of aspergillus on rice.		1979	114865
[formation of volutin inclusions in the mycelium of aspergilli growing on media with hydrocarbons].		1979	440149