Publications

fish oil controversy.		2009	2718495
[the mineral contents of helminths and arthropods. their study].		2009	4406406
development of personality and intelligence in children with congenital heart disease.	one hundred and twenty-eight children with congenital heart disease (chd) and 89 healthy controls were compared according to their development of personality and intelligence. presence of physical disability in daily life was the independent variable for dividing children with chd into two groups: children with physical handicap due to chd (n = 77) have lower iq scores than healthy children. unexpectedly, in the parents' opinion they show no difference in behaviour patterns. on introspective exa ...	2009	2708467
coordination of mural elements and myofilaments during arteriolar constriction.	arterioles undergo major morphological changes during vasoconstriction. we used transmission electron microscopy to study wall morphology in both dilated and constricted microvessels to understand the cellular basis of these changes. the relation between the orientation and density of myofilaments and the distribution of dense bodies was analyzed with respect to the level of microvessel tone. the data show a strong correlation between the degree of arteriolar constriction and both the orientatio ...	2009	3815757
clinical use of differential lung ventilation in the treatment of asymmetric lung injury: report of a case.	successful treatment of a 26-year-old male with asymmetric lung injury following a traffic accident is described. the patient was admitted with head injuries, right lung contusion, right pneumothorax, and multiple rib fractures (right 1-9). he developed a left pneumohemothorax and complete atelectasis of the lower lobe of the left lung two days later. conventional mechanical ventilation with and without positive end-expiratory pressure (peep), supplemented by repeated therapeutic bronchoscopy, f ...	2009	2818713
[construction and testing of a strain gauge for measuring masticatory force].	the main objective of this study was to establish and test a measuring system for monitoring occlusal forces and force cycles during mastication. a measuring module using strain gauge transducers was incorporated in the bridge-pontic of a three part test bridgework. a load cell was used intraorally for loading and calibration. the test bridges were incorporated in the patient's mouth and calibrated in vivo using variable loads. the recording system was a special intensifying and processing syste ...	2009	2772595
[follow-up results after sta-mca anastomosis in cases of middle cerebral artery stenosis].	to elucidate hemodynamic changes and prognosis after sta-mca anastomosis, thirteen cases with middle cerebral artery stenosis were systematically analyzed from the pre- and postoperative findings of cerebral angiography, ct scan and rcbf. they were followed up about 3 years long on the average postoperatively. postoperative angiography revealed patent bypass in all cases. no remarkable changes at the site of stenosis were observed in the group a (6 in 13 cases), who had angiographically sufficie ...	2009	3627366
[ultrasonic diagnosis of tumorous lesions of the liver].		2009	2698513
simulation of ionic transport mediated by membrane proteins for educational purposes.	an interactive pascal program for use in the ibm-pc and compatible computers is presented. it simulates the activation of integral membrane proteins involved in ionic transport. the program allows variation of the number of transport proteins, ionic concentrations, permeability ratios, voltage dependence of permeabilities and temperature. a separate procedure for simulation of na+-k+ pumps has been included considering their relevance in physiology. the main purpose of the present program is to ...	2009	2653720
repeated hepatic ischemia as a treatment for carcinoid liver metastases.	hepatic ischemia has been used in the treatment of bilobar malignant carcinoid tumors in the liver. hepatic artery ligation, hepatic dearterialization, and embolization with nondegradable micromaterial have been followed by collateral formation and are associated with a high complication rate. to reduce this, an implantable vascular occluder permitting intermittent occlusion of the hepatic artery was used in 3 patients with bilateral malignant carcinoid in the liver, with high urinary excretion ...	2009	2741468
exocrine pancreatic function after gastrectomy. specificity of indirect tests.	we compared intraindividually the specificity of indirect pancreatic function tests before and after total (n = 4; roux-en-y) or subtotal (n = 6; billroth ii) gastrectomy. before gastrectomy only 1 patient showed a falsely pathological result with the pancreolauryl test (90% specificity), while the results of all the other tests were correctly normal (100% specificity using the usual cutoff limits). after gastrectomy the respective specificities were as follows: pancreolauryl test 10%, bentiromi ...	2009	2656361
exocrine pancreatic function after gastrectomy. specificity of indirect tests.	we compared intraindividually the specificity of indirect pancreatic function tests before and after total (n = 4; roux-en-y) or subtotal (n = 6; billroth ii) gastrectomy. before gastrectomy only 1 patient showed a falsely pathological result with the pancreolauryl test (90% specificity), while the results of all the other tests were correctly normal (100% specificity using the usual cutoff limits). after gastrectomy the respective specificities were as follows: pancreolauryl test 10%, bentiromi ...	2009	2656361
european collaborative trial of multifactorial prevention of coronary heart disease.		2009	2882103
maternal mortality--a neglected tragedy. where is the m in mch?		2009	2861534
evidence for subgroups of adextrals based on speech lateralization and cognitive patterns.	some non-right-handers may prefer the left hand by virtue of well-organized or high levels of spatial ability, vested primarily in the right hemisphere, rather than as a result of anomalous speech representation. this study tested the hypothesis that adextral (left-handed or ambidextrous) individuals with high spatial ability might have cerebral speech lateralization patterns similar to dextrals. paper-and-pencil tests of various cognitive abilities, a verbal dichotic listening task, and a hande ...	2009	2771036
enzyme linked immunosorbent assay for measuring antithrombin iii.	a competitive elisa for the measurement of antithrombin iii antigen was developed using a pure preparation of antithrombin. this two step immunoassay requires only one antibody binding step and avoids the binding of anti-antithrombin antibody to the solid phase. plasma samples measured by this elisa correlated well with measurements taken with immune electrophoresis measurements (r = 0.933); the elisa was also shown to be both sensitive and precise.	2009	2794088
bone gla protein (bgp) levels and bone turnover in rheumatoid arthritis.	bone gla protein (bgp) and other biochemical indices of bone turnover were measured in 42 female patients with rheumatoid arthritis (ra) and in a group of normal subjects matched for sex and age. mean serum bgp concentrations were significantly higher in patients with active arthritis than in patients with mild activity (p less than 0.01) and controls (p less than 0.01). no significant difference was found in serum bgp levels and in other parameters of bone turnover when the patients were strati ...	2009	2786438
mucopolysaccharide histochemistry of musca domestica. x. the malpighian tubules.		2009	4131714
[golabi rosen syndrome: a rare syndrome with imprecise nosologic limits, failure of prenatal diagnosis].		2009	2746174
development and use of asbestos risk estimates.	several groups of researchers, at the request of government agencies in various countries, have reviewed the literature on the health effects of asbestos exposure and derived quantitative estimates of the risks from such exposures. because of differences in the area of responsibility of the different agencies, these estimates have inevitably been based on different assumptions concerning exposure (concentration, number of years of exposure, initial age, and number of hours per week). the lifetim ...	2009	2744842
reticulospinal neurons, locomotor control and the development of tailswimming in xenopus.	the development of early swimming in xenopus occurs early during the embryonic period and within a few hours. between stages 25 and 33 the central nervous system reaches a state of 'critical mass' at which the for swimming necessary body structures have partly developed, thus enabling the embryo to move through the water. the pattern of undulatory body movements is formed within the pattern generators in the central nervous system (cns) involving different types of neurons in the spinal cord and ...	2009	3077003
levels of adult alcohol consumption induced by interactions with child confederates exhibiting normal versus externalizing behaviors.	investigated levels of distress and alcohol consumption in ss interacting with problematic vs non-problematic child confederates. social drinkers were randomly assigned to interact with boys trained to enact behaviors characteristic of either normal or attention-deficit disorder/conduct disorder (add/cd) children. mood data were collected before and after the interaction. ss engaged in ad lib beer consumption for 20 min, while anticipating another interaction with the same boy. children in the a ...	2009	2768665
creatine kinase mb and m-mode echocardiographic changes in cardiac contusion.	cardiac contusion was suspected in 95 patients with severe blunt chest trauma of whom 93 did not require hospitalization. creatine kinase (ck) mb isoenzyme activity was elevated over 4.0 u/l in 10 (10.5%) patients (ck mb positive) by between 2% and 8% (mean +/- se 4.1 +/- 0.6%), when the total ck was 296 +/- 74 (mean +/- se). two patients had a pericardial rub. m-mode echocardiography was performed on 16 patients: 7 ck mb positive (ck mb+) and 9 ck mb negative (ck mb-) without the physician's kn ...	2009	4096487
[doppler ultrasonographic study of venous diseases of the legs].		2009	2949409
cancer risks due to occupational exposure to formaldehyde: results of a multi-site case-control study in montreal.	a case-control study was undertaken in montreal to investigate the possible associations between occupational exposures and cancers of the following sites: oesophagus, stomach, colo-rectum, liver, pancreas, lung, prostate, bladder, kidney, melanoma and lymphoid tissue. in total, 3,726 cancer patients and 533 population controls were interviewed to obtain detailed lifetime job histories and information on potential confounders. each job history was translated into a history of occupational exposu ...	2009	2744897
[clinico-diagnostic significance of enzyme activity determinations].		2009	2697998
renal function, glycemic control and perindopril in diabetic patients.	we studied the effect of perindopril, administered for a period of 9 months, on renal function, albuminuria and glycemic control of diabetic subjects with mild-to-moderate hypertension. after 1 month of placebo, 40 insulin-treated patients were divided into 3 groups based on the level of albuminuria. group i had normal albuminuria (less than 15 mg/24 hr), group ii had pathological microalbuminuria (15-150 mg/24 hr) and group iii had macroproteinuria (greater than 150 mg/24 hr). they were given p ...	2009	2691128
decreased plasma c-peptide to insulin molar ratio after oral glucose in elderly subjects.	to evaluate the effect of aging on hepatic extraction of insulin, peripheral plasma insulin and c-peptide levels after an oral glucose ingestion were measured in 39 non-obese healthy subjects with normal glucose tolerance test. the subjects were divided into two groups. one age group was below 30 years (n = 17) and another was above 50 years (n = 22). blood glucose level was significantly higher in elderly subjects than in young at 30 and 60 min, and insulin level was also significantly higher i ...	2009	2698354
letter: pretending to be unconscious.		2009	4131204
minaprine: an anticholinergic-free antidepressant? results of a controlled trial of mianserin.	minaprine is an amino-phenylpyridazine antidepressant reported to be relatively free of anticholinergic effects, cardiotoxicity, drowsiness, and weight gain. minaprine (100 mg t.d.s. and 100 mg b.d.) was compared with mianserin (20 mg t.d.s.) in 117 depressed patients, and all three regimes produced significant reductions in scores on the hrsd at the end of six weeks' treatment. there were no anticholinergic effects, but there was a significantly greater incidence of drowsiness with mianserin th ...	2009	2690999
layer thickness in rotational panoramic radiography: some specific aspects.	it is customary to define the thickness of the image layer in rotational panoramic radiography along the central ray of the x-ray beam. this results in calculated values which overestimate its thickness, especially in regions where the central ray deviates markedly from orthogonality. a supplementary definition is proposed which considers layer thickness in a direction perpendicular to the central plane of the image layer. this provides values which may be more easily related to the thickness of ...	2009	2637877
performance and well-being under tilting conditions: the effects of visual reference and artificial horizon.	it is generally agreed that the incidence of motion sickness in sailors working below deck is higher than in sailors who have the horizon as a visual reference on the bridge. this study investigated the possible beneficial effect of a projected artificial horizon as a means to prevent seasickness. twelve subjects were exposed to angular motion in a tilting room under three experimental conditions: a) with the windows covered, allowing no visual reference from the outside world; b) with the windo ...	2009	2775135
[distribution pattern and risk factors of lung cancer in qidong rural area].	distribution pattern, epidemiologic trend and risk factors of lung cancer in rural area were studied and analysed based on the data of qidong cancer registry (1972-1986), of sampling survey of cigarette smoking rate among rural residents, and of consumption of cigarette and fuel in qidong. the results showed that: 1. lung cancer mortality had increased from 9.00/10(5) in 1972 to 20.03/10(5) in 1986 with annual increase rate of 5.88%; 2. lung cancer mortality in 1980s (1984-1986) was increased by ...	2009	2620633
sleep apnea in alzheimer's disease.	mental deterioration accompanying sleep apnea has been noted frequently. because sleep apnea increases with age, such deficits raise the possibility that dementia in the elderly could be related to sleep apnea. in this study we investigated this possibility cross-sectionally by comparing respiration during sleep in 28 patients with alzheimer's disease (ad) and 25 nondemented controls. we hypothesized that higher levels of sleep apnea would be present in ad patients. our results indicated no sign ...	2009	2812195
sleep apnea in alzheimer's disease.	mental deterioration accompanying sleep apnea has been noted frequently. because sleep apnea increases with age, such deficits raise the possibility that dementia in the elderly could be related to sleep apnea. in this study we investigated this possibility cross-sectionally by comparing respiration during sleep in 28 patients with alzheimer's disease (ad) and 25 nondemented controls. we hypothesized that higher levels of sleep apnea would be present in ad patients. our results indicated no sign ...	2009	2812195
[studies on the constituents of trichosanthes root. ii. constituents of roots of trichosanthes cucumeroides maxim].	from the fresh roots of trichosanthes cucumeroides maxim., three new triterpenoid glycosides with a bitter taste were obtained together with methyl palmitate, palmitic acid, alpha-spinasterol, stigmast-7-en-3 beta-ol, alpha-spinasterol 3-o-beta-d-glucopyranoside, stigmast-7-en-3 beta-ol 3-o-beta-d-glucopyranoside and vanillic acid. on the basis of the results of spectral and chemical investigations, these glycosides were characterized as 25-o-beta-d-glucopyranosyl-11-oxocucurbit-5-ene-3 beta,24( ...	2009	2760812
a method to detect the in vitro modification of serum beta-2-microglobulin in health and disease.	the electrophoretically fast moving form of beta-2-microglobulin (alpha-b2m) was shown by crossed immunoelectrophoresis to be produced in vitro after incubation at room temperature and was not present in freshly drawn sera. a radioisotopic assay was developed to measure this conversion to alpha-b2m in sera from 14 healthy individuals and from 208 patients with benign and malignant disease. the activity was present in all the sera tested and the levels were significantly higher in sera from patie ...	2009	2846212
a method to detect the in vitro modification of serum beta-2-microglobulin in health and disease.	the electrophoretically fast moving form of beta-2-microglobulin (alpha-b2m) was shown by crossed immunoelectrophoresis to be produced in vitro after incubation at room temperature and was not present in freshly drawn sera. a radioisotopic assay was developed to measure this conversion to alpha-b2m in sera from 14 healthy individuals and from 208 patients with benign and malignant disease. the activity was present in all the sera tested and the levels were significantly higher in sera from patie ...	2009	2846212
autologous bone marrow transplantation in patients with aml in second complete remission (cr).		2009	2653505
[incidence of anemia in first-time blood donors].		2009	3726715
microbial interactions influence inflammatory host cell responses.	the inflammatory response plays an important role in the tissue destruction associated with periodontitis. bacterial species can regulate the inflammatory responses of host cells, triggered by pathogens. it was hypothesized that, in the field of oral microbiology/immunology, such effects of bacterial interactions on inflammatory host cell responses might also be present. in this study, the effects of beneficial, commensal, and pathogenic species on aggregatibacter actinomycetemcomitans-induced i ...	2009	19828891
involvement of cytosolic phospholipase a2alpha in mmp-9 up-regulation.	matrix metalloproteinase-9 (mmp-9) is important in the pathogenesis of periodontitis. cytosolic phospholipase a(2)alpha (cpla(2)alpha) is involved in mmp-9 up-regulation in human monocytes. we tested the hypothesis that cpla(2)alpha also regulates mmp-9 induction by fusobacterium nucleatum and by phorbol 12-myristate-13-acetate (pma) in gingival epithelial cells. while pma induced mmp-9 expression considerably, f. nucleatum did so moderately. this time-course study demonstrated that mmp-9 mrna u ...	2009	19828892
progression of chronic periodontitis can be predicted by the levels of porphyromonas gingivalis and treponema denticola in subgingival plaque.	chronic periodontitis is an inflammatory disease of the supporting tissues of the teeth associated with bacteria. diagnosis is achieved retrospectively by clinical observation of attachment loss. predicting disease progression would allow for targeted preventive therapy. the aim of this study was to monitor disease progression in patients on a maintenance program and determine the levels of specific bacteria in subgingival plaque samples and then examine the ability of the clinical parameters of ...	2009	19832799
characterization of bacterial flora in persistent apical periodontitis lesions.	microorganisms are able to survive and induce persistent infection in periapical tissues. the aim of this study was to investigate the composition of the microflora of persistent apical periodontitis lesions.	2009	19832803
autoaggregation response of fusobacterium nucleatum.	fusobacterium nucleatum is a gram-negative oral bacterial species associated with periodontal disease progression. this species is perhaps best known for its ability to adhere to a vast array of other bacteria and eukaryotic cells. numerous studies of f. nucleatum have examined various coaggregation partners and inhibitors, but it is largely unknown whether these interactions induce a particular genetic response. we tested coaggregation between f. nucleatum atcc strain 25586 and various species ...	2009	19837836
antibiotic resistance of periodontal pathogens obtained from frequent antibiotic users.	the occurrence of bacterial strains resistant to different antimicrobials is a growing problem in latin american countries. the aim was to measure the antimicrobial susceptibility of fusobacterium nucleatum, prevotella intermedia and prophyromona gingivalis, isolated from chronic periodontitis patients. twenty-five patients diagnosed with generalized- moderate or advanced- chronic periodontitis were consecutively recruited from patients attending the periodontal specialist program, javeriana uni ...	2009	19839485
hydrogen sulfide production from cysteine and homocysteine by periodontal and oral bacteria.	hydrogen sulfide is one of the predominant volatile sulfur compounds (vscs) produced by oral bacteria. this study developed and evaluated a system for detecting hydrogen sulfide production by oral bacteria.	2009	19905954
sonic extracts from a bacterium related to periapical disease activate gelatinase a and inactivate tissue inhibitor of metalloproteinases timp-1 and timp-2.	to examine the effects of sonicated bacterial extracts (sbes) from three related to periapical disease bacteria (porphyromonas gingivalis, p. endodontalis and f. nucleatum) on the activation of matrix metalloproteinase (mmp-2) and the inactivation of tissue inhibitors of metalloproteinase (timp-1 and timp-2).	2009	19912382
assessment of intraradicular bacterial composition by terminal restriction fragment length polymorphism analysis.	the aim of the study was to assess the bacterial community structures associated with endodontic infections using terminal restriction fragment length polymorphism (t-rflp), and to investigate the correlation of whole community profiles with the manifestation of particular clinical features.	2009	19702949
oral bacteria induce a differential activation of human immunodeficiency virus-1 promoter in t cells, macrophages and dendritic cells.	the human immunodeficiency virus (hiv) can integrate into t cells, macrophages and dendritic cells resulting in a latent infection. reports have also demonstrated that various microbial and host cell factors can trigger hiv reactivation leading to hiv recrudescence, potentially undermining highly active antiretroviral therapies.	2009	19702954
the effect of lactoferrin on oral bacterial attachment.	lactoferrin (lf), an iron-binding salivary glycoprotein, plays an important role in human innate defense against local mucosal infection. we hypothesized that lf interferes with initial oral bacterial attachment to surfaces by iron sequestration, so inhibiting subsequent biofilm formation. the objective was to investigate the effect of lf on the early stages of single-species and multi-species oral biofilm development.	2009	19702956
contemporary root canal irrigants are able to disrupt and eradicate single- and dual-species biofilms.	clinical/microbiological studies have consistently revealed the persistence of some bacteria after conventional root canal debridement. although this was originally attributed to the complexity of the root canal anatomy and the difficulty of delivering antibacterial agents effectively, it has emerged that the biofilm encasement of bacterial cells may confer a further mechanism of resistance. the purpose of this study was to investigate the relative disruption and bactericidal effects of root can ...	2009	19720223
maternal periodontal disease and soluble fms-like tyrosine kinase-1 expression.	this study was conducted to examine the relationship between maternal periodontal disease and plasma angiogenic factor expression of soluble fms-like tyrosine kinase (sflt)-1.	2009	19722802
fusobacterium nucleatum endocarditis mimicking polymyalgia rheumatica.	a case of fusobacterium nucleatum endocarditis in an 80-year-old man is reported. the patient presented with a headache and nonspecific musculoskeletal symptoms and was misdiagnosed as having polymyalgia rheumatica. the diagnosis of bacterial endocarditis was delayed because of an insidious presentation, typical in infections with low virulence micro-organisms. the musculoskeletal symptoms, unresponsive to protracted corticosteroids, completely resolved with intravenous ampicillin treatment. rhe ...	2009	19738533
mutualistic biofilm communities develop with porphyromonas gingivalis and initial, early, and late colonizers of enamel.	porphyromonas gingivalis is present in dental plaque as early as 4 h after tooth cleaning, but it is also associated with periodontal disease, a late-developing event in the microbial successions that characterize daily plaque development. we report here that p. gingivalis atcc 33277 is remarkable in its ability to interact with a variety of initial, early, middle, and late colonizers growing solely on saliva. integration of p. gingivalis into multispecies communities was investigated by using t ...	2009	19749049
[preliminary study on the discrimination of putative periodontal pathogens with a metabonomics method].	to evaluate the feasibility of identifying oral pathogenic bacteria by comparing the metabolic profiling of putative periodontal pathogens and try to find a convenient and rapid way to discriminate oral microorganisms.	2009	19637485
bone microbial contamination influences autogenous grafting in sinus augmentation.	the oral occurrence of putative microbial pathogens in humans has been documented in health and disease. the presence of periodontopathogens in patients with a history of periodontal disease may have a negative impact on bone regeneration. this investigation was conducted to confirm the presence of periodontal pathogens in bone particles harvested intraorally for maxillary sinus augmentation and to assess the clinical and radiographic outcomes 6 to 12 months after bone augmentation.	2009	19656037
quantitative detection of periodontopathic bacteria in atherosclerotic plaques from coronary arteries.	oral pathogens, including periodontopathic bacteria, are thought to be aetiological factors in the development of cardiovascular disease. in this study, the presence of aggregatibacter actinomycetemcomitans, fusobacterium nucleatum-periodonticum-simiae group, porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens and tannerella forsythia in atheromatous plaques from coronary arteries was determined by real-time pcr. forty-four patients displaying cardiovascular disease were submi ...	2009	19679682
a novel vaccine targeting fusobacterium nucleatum against abscesses and halitosis.	an abscess in a gum pocket, resulting from bacterial infection, is a common source of chronic halitosis. although antibiotics are generally prescribed for abscesses, they require multiple treatments with risks of creating resistant bacterial strains. here we develop a novel vaccine using ultraviolet-inactivated fusobacterium nucleatum (f. nucleatum), a representative oral bacterium for halitosis. a gum pocket model, established by continuous inoculation of f. nucleatum, was employed to validate ...	2009	19162109
new bacterial compositions in root-filled teeth with periradicular lesions.	the aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. specimens were sampled from patients undergoing root canal retreatment. the bacteria were characterized by morphologic and biochemical analysis and by 16s rrna gene sequencing. microorganisms were detected in 10 of 18 teeth. the majority of positive samples revealed a mixed culture of 2-8 species. in 2 teeth enterococcus faecalis was the only detected species. for the first ...	2009	19166766
coenzyme recognition and gene regulation by a flavin mononucleotide riboswitch.	the biosynthesis of several protein cofactors is subject to feedback regulation by riboswitches. flavin mononucleotide (fmn)-specific riboswitches, also known as rfn elements, direct expression of bacterial genes involved in the biosynthesis and transport of riboflavin (vitamin b(2)) and related compounds. here we present the crystal structures of the fusobacterium nucleatum riboswitch bound to fmn, riboflavin and antibiotic roseoflavin. the fmn riboswitch structure, centred on an fmn-bound six- ...	2009	19169240
effect of dietary omega-3 polyunsaturated fatty acids on experimental periodontitis in the mouse.	periodontitis is an infective disease caused predominantly by gram-negative anerobes. the host inflammatory response to these bacteria causes alveolar bone loss, which characterizes periodontitis. omega-3 polyunsaturated fatty acids have recognized anti-inflammatory effects; their oxygenated derivatives are key mediators in reducing inflammation. in this study we tested the hypothesis that dietary supplementation with tuna fish oil rich in the n-3 polyunsaturated fatty acid, docosahexaenoic acid ...	2009	19210341
does pregnancy have an impact on the subgingival microbiota?	we investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care.	2009	19228092
purulent pericarditis in patient with esophageal cancer presenting with cardiac tamponade.		2009	19272728
an in vitro evaluation of hydrolytic enzymes as dental plaque control agents.	the plaque-control potential of commercially available amylase, lipase and protease was evaluated by observing their effects on coaggregation and on bacterial viability within various plaque microcosms. a quantitative coaggregation assay indicated that protease significantly inhibited the extent of coaggregation of actinomyces naeslundii and streptococcus oralis (p <0.05) and of porphyromonas gingivalis and s. oralis. amylase significantly (p <0.05) increased the coaggregation of a. naeslundii v ...	2009	19273645
fusobacterium nucleatum atcc 10953 requires actinomyces naeslundii atcc 43146 for growth on saliva in a three-species community that includes streptococcus oralis 34.	formation of dental plaque is a developmental process involving initial and late colonizing species that form polymicrobial communities. fusobacteria are the most numerous gram-negative bacteria in dental plaque, but they become prevalent after the initial commensal colonizers, such as streptococci and actinomyces, have established communities. the unusual ability of these bacteria to coaggregate with commensals, as well as pathogenic late colonizers, has been proposed to facilitate colonization ...	2009	19286780
tlr2 sensing of f. nucleatum and s. sanguinis distinctly triggered gingival innate response.	gingival tissue faces constant exposure to micro-organisms. it functions as part of the host response, an anti-microbial barrier that recognizes and discriminates between commensal and pathogenic bacteria. this study aimed to evaluate and compare the effects of cell wall extracts from different periodontal bacteria, commensals streptococcus sanguinis and fusobacterium nucleatum and the pathogen porphyromonas gingivalis, on the innate immune response of gingival keratinocytes and the role of tlr2 ...	2009	19299164
characterization of the complex bacterial communities colonizing biliary stents reveals a host-dependent diversity.	this study provides a comprehensive survey of the spatial and temporal bacterial composition of biliary stent biofilms. the bacterial diversity, distribution and dynamics of 59 biliary and 4 pancreatic stent communities from 40 patients being treated at two different hospitals, which implant stents either simultaneously or consecutively, were characterized by single-strand conformation polymorphism (sscp) analysis. fifty-one phylotypes belonging to 5 bacterial phyla and 24 bacterial families wer ...	2009	19360025
bacterial culture and dna checkerboard for the detection of internal contamination in dental implants.	the aim of this in vitro study was to evaluate the bacterial leakage along the implant-abutment interface by the conventional bacterial culture and dna checkerboard hybridization method.	2009	19374709
evidence for pseudoknot formation of class i preq1 riboswitch aptamers.	all in a knot. the smallest riboswitch forms a pseudoknot in solution. this is demonstrated for preq(1) class i aptamers by mutational analysis in combination with (1)h nmr-based structure probing. how pseudoknot formation mediates the mrna response through its expression platform is now open for investigation.	2009	19382115
are putative periodontal pathogens reliable diagnostic markers?	periodontitis is one of the most common chronic inflammatory diseases. a number of putative bacterial pathogens have been associated with the disease and are used as diagnostic markers. in the present study, we compared the prevalence of oral bacterial species in the subgingival biofilm of generalized aggressive periodontitis (gap) (n = 44) and chronic periodontitis (cp) (n = 46) patients with that of a periodontitis-resistant control group (pr) (n = 21). the control group consisted of subjects ...	2009	19386852
the oxyr homologue in tannerella forsythia regulates expression of oxidative stress responses and biofilm formation.	tannerella forsythia is an anaerobic periodontal pathogen that encounters constant oxidative stress in the human oral cavity due to exposure to air and reactive oxidative species from coexisting dental plaque bacteria as well as leukocytes. in this study, we sought to characterize a t. forsythia orf with close similarity to bacterial oxidative stress response sensor protein oxyr. to analyse the role of this oxyr homologue, a gene deletion mutant was constructed and characterized. aerotolerance, ...	2009	19389765
complementation of the fada mutation in fusobacterium nucleatum demonstrates that the surface-exposed adhesin promotes cellular invasion and placental colonization.	fusobacterium nucleatum is a gram-negative oral anaerobe implicated in periodontal disease and adverse pregnancy outcome. the organism colonizes the mouse placenta, causing localized infection and inflammation. the mechanism of placental colonization has not been elucidated. previous studies identified a novel adhesin from f. nucleatum, fada, as being involved in the attachment and invasion of host cells. the fada deletion mutant f. nucleatum 12230 us1 was defective in host cell attachment and i ...	2009	19398541
the impact of the stone age diet on gingival conditions in the absence of oral hygiene.	the objective of this study was to assess the oral microbiota and clinical data in subjects without access to traditional oral hygiene methods and who ate a diet available in the stone age.	2009	19405829
decreased interleukin-2 responses to fusobacterium nucleatum and porphyromonas gingivalis in generalized aggressive periodontitis.	compromised t-cell responses to periodontal pathogens may contribute to the pathogenesis of generalized aggressive periodontitis (gagp). in this study, we attempted to characterize t-helper cell (th1, th2, and th17) responses in patients with gagp and healthy controls upon stimulation with disease-relevant pathogens.	2009	19405834
tgf-beta1 inhibits tlr-mediated odontoblast responses to oral bacteria.	tgf-beta1 exerts diverse functions in tooth development and tissue repair, but its role in microbial defenses of the tooth is not well-understood. odontoblasts extending their cellular processes into the dentin are the first cells to recognize signals from tgf-beta1 and bacteria in carious dentin. this study aimed to determine the role of tgf-beta1 in modulating odontoblast responses to oral bacteria. we show that these responses depend upon the expression levels of microbial recognition recepto ...	2009	19407153
multi-species biofilm formation on dental materials and an adhesive patch.	this laboratory study assessed the influence of surface roughness and contact time on the formation of a multi-species biofilm on dental materials (adhesive patch, composite, amalgam and enamel).	2009	19408815
human cytomegalovirus and epstein-barr virus inhibit oral bacteria-induced macrophage activation and phagocytosis.	periodontal disease is an inflammatory condition caused by periodontal microorganisms. viruses such as human cytomegalovirus (hcmv) and epstein-barr virus (ebv) are associated with certain types of periodontal disease, but their roles in promoting the disease are unclear. because both viruses infect human macrophages, cells which play key roles in the clearance of pathogenic bacteria, it is likely that the viruses alter the functional capacity of macrophages by inhibiting their defense mechanism ...	2009	19416455
polymicrobial infections, biofilms, and beyond.		2009	19419439
mouse model of experimental periodontitis induced by porphyromonas gingivalis/fusobacterium nucleatum infection: bone loss and host response.	to compare the effect of oral infection with porphyromonas gingivalis or fusobacterium nucleatum versus infection with both bacteria on mouse periodontal tissues, and to characterize the inflammatory response.	2009	19419440
purification and partial characterization of a bacteriocin produced by an oral fusobacterium nucleatum isolate.	to purify and partially characterize a bacteriocin produced by a fusobacterium nucleatum strain.	2009	19426276
bacteria in the apical root canal of teeth with primary apical periodontitis.	bacteria settled in the apical root canal are in a privileged position to inflict damage to the periradicular tissues. therefore, the species identified in this region can be of special relevance for the pathogenesis of apical periodontitis. this study investigated the occurrence and levels of several bacterial taxa in the apical root canal of teeth with apical periodontitis.	2009	19426923
proteomics of porphyromonas gingivalis within a model oral microbial community.	porphyromonas gingivalis is a periodontal pathogen that resides in a complex multispecies microbial biofilm community known as dental plaque. confocal laser scanning microscopy showed that p. gingivalis can assemble into communities in vitro with streptococcus gordonii and fusobacterium nucleatum, common constituents of dental plaque. whole cell quantitative proteomics, along with mutant construction and analysis, were conducted to investigate how p. gingivalis adapts to this three species commu ...	2009	19454014
methods for optimizing dna extraction before quantifying oral bacterial numbers by real-time pcr.	methods for the optimal extraction of genomic dna for real-time pcr enumeration of oral bacteria using the muramidase, mutanolysin, were developed using a simple in vitro oral flora model comprised of the facultative anaerobic gram-positive bacteria, lactobacillus acidophilus and streptococcus mutans, the gram-positive anaerobe, parvimonas micra, and the gram-negative anaerobes, porphyromonas gingivalis, prevotella melaninogenica and fusobacterium nucleatum. traditional, as well as more elaborat ...	2009	19459962
fusobacterium nucleatum, the first gram-negative bacterium demonstrated to produce polyglutamate.	poly-gamma-glutamate has been described in many gram-positive organisms. when anchored to the surface, it is a capsule and as such a virulence factor. based on sequence similarities, few gram-negative organisms have been suggested to synthesize poly-gamma-glutamate. for the first time, a gram-negative bacterium, fusobacterium nucleatum, is shown to produce and secrete poly-gamma-glutamate. putative poly-gamma-glutamate-synthesizing genes from gram-negative organisms have been compared with their ...	2009	19483793
fetal exposure to oral pathogens and subsequent risk for neonatal intensive care admission.	maternal periodontal infection has been associated with adverse maternal and neonatal outcomes. in utero fetal exposure to oral pathogens was also recognized as deleterious to the fetus. the objective of this study was to determine the relationship between fetal exposure to oral pathogens and neonatal intensive care unit (nicu) admission.	2009	19485816
systemic immune responses in pregnancy and periodontitis: relationship to pregnancy outcomes in the obstetrics and periodontal therapy (opt) study.	our previous studies reported on the obstetric, periodontal, and microbiologic outcomes of women participating in the obstetrics and periodontal therapy (opt) study. this article describes the systemic antibody responses to selected periodontal bacteria in the same patients.	2009	19485826
[detection of luxs gene of quorum sensing signal system in periodontal pathogen].	to detect the presence and distribution of luxs gene in quorum sensing signal system in the periodontal pathogens.	2009	19489256
bacterial colonization of enamel in situ investigated using fluorescence in situ hybridization.	oral biofilms are one of the greatest challenges in dental research. the present study aimed to investigate initial bacterial colonization of enamel surfaces in situ using fluorescence in situ hybridization (fish) over a 12 h period. for this purpose, bovine enamel slabs were fixed on buccal sites of individual splints worn by six subjects for 2, 6 and 12 h to allow biofilm formation. specimens were processed for fish and evaluated with confocal laser-scanning microscopy, using probes for eubact ...	2009	19528150
starvation response and growth in serum of fusobacterium nucleatum, peptostreptococcus anaerobius, prevotella intermedia, and pseudoramibacter alactolyticus.	the microbiota inhabiting the untreated root canal differ markedly from those found in post-treatment disease, yet there is limited information on the microbial characteristics distinguishing the different infections. we hypothesized that starvation survival is a key microbial property in species selection. this study analyzed starvation-survival behavior over 60 days of species representative of the untreated root canal infection: fusobacterium nucleatum, peptostreptococcus anaerobius, prevotel ...	2009	19540450
a 21-year-old man with fever and abdominal pain after recent peritonsillar abscess drainage.	lemierre syndrome an extremely rare and unreported disease typically results from oropharyngeal infection and/or local tissue trauma with invasion of the parapharyngeal soft tissue with bacteria. once local tissue invasion occurs, internal jugular vein septic thrombophlebitis with or without metastatic complications may occur. the etiology of lemierre syndrome is generally fusobacterium necrophorum, reported to be present in approximately 80% of the cases. the outcome of lemierre syndrome in the ...	2009	19555636
real-time polymerase chain reaction to determine the prevalence and copy number of epstein-barr virus and cytomegalovirus dna in subgingival plaque at individual healthy and periodontal disease sites.	detection of cytomegalovirus (cmv) and epstein-barr virus (ebv) in plaque from patients with periodontal disease provides support for the theory that these viruses play a role in the pathogenesis of periodontitis. this study sought to further define this relationship by determining the prevalence of these viruses at individual disease and healthy sites of patients with periodontal disease and to determine whether the presence and amount of viral dna correlate with disease severity.	2009	19563294
fusobacterium nucleatum enters normal human oral fibroblasts in vitro.	fusobacterium nucleatum, a commensal opportunistic oral bacterium, is capable of invading gingival epithelial cells, but the entrance into human primary oral fibroblast cells has not been documented. this study evaluated the ability of three strains of f. nucleatum (f. nucleatum ssp. nucleatum, f. nucleatum ssp. polymorphum, and f. nucleatum ssp. vincentii) to enter gingival fibroblasts (gfs) and periodontal ligament fibroblasts (plfs).	2009	19563299
comparison between polymerase chain reaction-based and checkerboard dna hybridization techniques for microbial assessment of subgingival plaque samples.	to compare polymerase chain reaction (pcr) with subsequent reverse hybridization (micro-ident test) and checkerboard dna-dna hybridization for the identification of 13 bacterial species in subgingival plaque samples.	2009	19563330
aggregatibacter actinomycetemcomitans builds mutualistic biofilm communities with fusobacterium nucleatum and veillonella species in saliva.	human oral bacterial pathogens grow in attached multispecies biofilm communities. unattached cells are quickly removed by swallowing. therefore, surface attachment is essential for growth, and we investigated multispecies community interactions resulting in mutualistic growth on saliva as the sole nutritional source. we used two model systems, saliva-coated transferable solid-phase polystyrene pegs (peg biofilms) and flow cells with saliva-coated glass surfaces. fluorescent antibody staining and ...	2009	19564387
proteomic analysis of endodontic infections by liquid chromatography-tandem mass spectrometry.	endodontic infections are very prevalent and have a polymicrobial etiology characterized by complex interrelationships between endodontic microorganisms and the host defenses. proteomic analysis of endodontic infections can provide global insights into the invasion, pathogenicity mechanisms, and multifactorial interactions existing between root canal bacteria and the host in the initiation and progression of apical periodontitis. the purpose of this study was to apply proteomic techniques such a ...	2009	19572900
photodynamic therapy as adjunct to non-surgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial.	recent preclinical and clinical data have suggested the potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, currently, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of the present study was to evaluate the clinical and microbiological effects of the adjunctive use of pdt in non-surgical periodontal treatment in patients receiving supportive periodontal therapy. twenty-fou ...	2009	18465191
in vitro bactericidal activity of recombinant human beta-defensin-3 against pathogenic bacterial strains in human tooth root canal.	human beta-defensin-3 (hbd3), an endogenous antimicrobial peptide, has strong broad-spectrum antimicrobial activity. this study aimed to obtain recombinant hbd3 (rhbd3) and to test the hypothesis that the antimicrobial characteristics of hbd3 may offer an advantage over conventional medicine in reducing intracanal bacteria. genetic engineering was used to obtain active rhbd3 and analysis revealed that it exhibited a broad spectrum of antibacterial activity at low micromolar concentrations agains ...	2009	18775647
granulocyte chemotactic protein 2 (gcp-2/cxcl6) complements interleukin-8 in periodontal disease.	mucosal inflammatory responses are orchestrated largely by pro-inflammatory chemokines. the chemokine granulocyte chemotactic protein 2 (cxcl6) is involved in neutrophil recruitment and migration. previous studies have shown that granulocyte chemotactic protein 2 is up-regulated during mucosal inflammation (e.g. in inflammatory bowel disease), similarly to the functionally and structurally related chemokine interleukin-8. nevertheless, unlike interleukin-8, a role of granulocyte chemotactic prot ...	2009	18842116
uncultivated bacteria as etiologic agents of intra-amniotic inflammation leading to preterm birth.	intra-amniotic infection and inflammation are major causes of preterm birth (ptb). however, intra-amniotic inflammation is often detected in the absence of infection. this may partly be due to the culturing methods employed in hospital laboratories, which are unable to detect the uncultivated species. in this study, intra-amniotic microbial infections associated with ptb were examined by both culture and 16s rrna-based culture-independent methods and were corroborated by the presence of intra-am ...	2009	18971361