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[dynamics of sexuality and sex customs]. 20113173040
absconders from a rural health centre in papua new guinea. 20113245337
absconders from a rural health centre in papua new guinea. 20113245337
vascularization of male gonads in blennius pavo (teleostei, blenniidae) as revealed by scanning electron microscopy of vascular corrosion casts.the vascular architecture of male gonads of blennius pavo is studied by scanning electron microscopy of vascular corrosion casts. arterial supply to the gonads is by a branch of the first ventral segmental artery of the tail. from the surface of the gonads, this vessel gives rise to branches which supply testes, spermatic ducts, testicular glands, blind pouches, urogenital sinus and urogenital papilla. the testis has a rope-ladder-like capillary network around the seminiferous tubules, while in ...20113238381
[aids. less risk for the circumcised]. 20113209172
nonlinear accumulation of propranolol enantiomers.the accumulation of (+)- and (-)-propranolol was investigated in nine subjects who received 160 mg of racemic propranolol as a single dose and then once daily for 7 days. the serum concentrations of propranolol enantiomers were measured by h.p.l.c. using a novel chiral stationary phase allowing direct resolution of underivatized propranolol. the (+)-propranolol auc increased from 412 +/- 223 ng ml-1 h after single doses (0-infinity) to 584 +/- 279 ng ml-1 h at steady-state (0-24 h) (p less than ...20113203053
fetomaternal macrotransfusion in the yorkshire region. 2. perinatal outcome.the perinatal outcome associated with fetomaternal macrotransfusion (fmmt), defined as fetomaternal haemorrhage greater than or equal to 10 ml, was investigated in a study group of 179 cases of fmmt and compared with the outcome in 322 controls selected from the same population. perinatal mortality and morbidity occurred more frequently in the case group (p less than 0.01). the mean apgar score at 1 min and the cord haemoglobin level differed significantly between the case and control groups (p ...20113207644
poona district leprosy committee annual report for 1985-86. 20113192982
solving the rod-and-frame test in a tachistoscopic presentation: effects of stimulus size and perceptual style. 20113226894
metabolism of natural and synthetic steroids used in cancer treatment. 20113279431
metabolism of natural and synthetic steroids used in cancer treatment. 20113279431
the ohio medicaid drug formulary. 20113328840
the ohio medicaid drug formulary. 20113328840
jurisprudent obstetrics. 20113176291
community based health care in the philippine highlands: the hanunuo mangyans of mindoro. 20113179104
a peculiar complication in le fort i osteotomy.in two cases of routine le fort i osteotomy in secondary cleft palate surgery, a swelling noted in the antral mucosa was biopsied. in both cases, histological examination resulted in a diagnosis of odontogenic myxoma. after analysis of the radiographs, it was concluded that the biopsies were taken from displaced tooth germs. the close histological similarity between myxoma on the one hand and dental papilla tissue, a thickened tooth follicle and a thickened or polypoid antral mucosal lining on t ...20113165391
paroxysmal unilateral dysosmia: a cured patient.a 31-year-old woman with an 11-year history of unilateral paroxysmal olfactory hallucinations was cured by resection of the homolateral olfactory bulb, which was believed to be the site of origin of the noxious smell.20113228277
biosynthesis of porphyrins and left ventricle function in patients with terminal renal insufficiency. 20113133291
[use of the o-nitrophenyl-beta-d-galactopyranoside test in the rapid diagnosis of strains of enterobacteria]. 20113130711
discordant effects of prednisone on anticardiolipin antibodies and the lupus anticoagulant. 20113094545
expression of genes in cloned murine cell lines that can be maintained in both interleukin 2- and interleukin 3-dependent growth states.two cloned murine cell lines, fd.c/1 and 32dcl-23 exhibit switching of lymphokine-dependent growth states. the bone marrow-derived fd.c/1 and 32dcl-23 cell lines are normally grown in culture medium supplemented with interleukin 3 (il3). the replacement of il3 with interleukin 2 (il2) in the medium results in an increase in il2 receptor expression in fd.c/1 and 32dcl-23 cells and the switching of cells to an il2-dependent growth state. we have compared patterns of protein and phosphoprotein synt ...20113111985
leasing firms seek out small and rural hospitals. 20113391576
antibiotic resistance of vibrio parahaemolyticus isolated from pond-reared litopenaeus vannamei marketed in natal, brazil.ten out of fifty fresh and refrigerated samples of shrimp (litopenaeus vannamei) collected from retailers in natal (rio grande do norte, northeastern brazil) tested positive for vibrio parahaemolyticus. the kanagawa test and multiplex pcr assays were used to detect tdh and trh hemolysins and the tdh, trh and tlh genes, respectively. all strains were kanagawa-negative and tlh-positive. antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. five strains ( ...201124031779
characterization of protease from alcaligens faecalis and its antibacterial activity on fish pathogens.alcaligens faecalis au01 isolated from seafood industry effluent produced an alkaline protease. the optimum culture conditions for growth as well as enzyme production were 37 degrees c and ph 8. the partially purified protease had specific activity of 9.66 with 17.77% recovery with the molecular weight of 33 kda and it was active between 30-70 degrees c and optimum being at 55 degrees c and ph 9. the enzyme retains more than 85% activity at 70 degrees c and 78% even at ph 10. the enzyme inhibite ...201122471216
[comparative study on the phenotypic characteristics and molecular typing of foodborne vibrio parahaemolyticus in guangdong province].to understand the phenotypic characteristics of foodborne vibrio parahaemolyticus in guangdong province through carrying out a comprehensive comparison including pulse field gel electrophoresis, ribotyping and serotyping.201122336613
[analysis on the etiological and molecular epidemiological characteristics of vibrio parahaemolyticus in beijing].to understand the etiological and molecular-epidemiological characteristics of vibrio parahaemolyticus in beijing.201122336612
[etiologic characteristics of vibrio parahaemolyticus strains causing outbreaks and sporadic cases in guangdong, 2009].to study the serotypes, virulence features and molecular characteristics of vibrio parahaemolyticus isolated in food poisoning cases and surveillance program on diarrhea patients in guangdong, 2009.201122336608
differences in stress response after uvc or uva irradiation in vibrio parahaemolyticus.the sos response is a global regulatory network for repairing dna damage induced by various environmental stresses such as uv irradiation. the escherichia coli sos response has been extensively studied. however, there are no reports on the sos response in vibrio parahaemolyticus. in this study, we examined the sos response in v. parahaemolyticus and compared the differential expression of genes induced by uvc and uva irradiation. in uvc-exposed wild-type cells, expression of several dna repair g ...201023766253
prevalence, serotyping and molecular characterization of vibrio parahaemolyticus in mussels from italian growing areas, adriatic sea.the aims of this study were to investigate the prevalence of total and pathogenic vibrio parahaemolyticus strains in italian mussels from different geographical areas of the adriatic sea and to determine their serotypes, toxigenic profiles and pandemic potential. out of 559 samples analysed during 2007, 65 (11.6%) were positive for v. parahaemolyticus. none of the isolates had the genes for thermostable direct haemolysin (tdh) and pandemic marker (toxrs), while five strains (7.7%) had that for t ...201023766016
detection of environmental vibrio parahaemolyticus using a polyclonal antibody by flow cytometry.the aim of this study was to detect and quantify vibrio parahaemolyticus using flow cytometry (fcm) in combination with a polyclonal antibody developed in our laboratory. experiments were carried out using v. parahaemolyticus cells in pure and mixed bacteria culture suspensions in either artificial or natural seawater. using fcm, v. parahaemolyticus cells labelled with the polyclonal antibody and a secondary fluorescein isothiocyanate-conjugated antibody were detected and rapidly quantified at l ...201023766012
serodiversity and ecological distribution of vibrio parahaemolyticus in the venetian lagoon, northeast italy.vibrio parahaemolyticus is a natural inhabitant of estuarine and marine environments constituting part of the autochthonous microflora. this species is associated with human gastroenteritis caused by ingestion of contaminated water and undercooked seafood. during the past several years, the number of v. parahaemolyticus gastroenteritis cases have increased worldwide, causing over half of all food-poisoning outbreaks of bacterial origin. vibrio populations in water are known to be influenced by e ...201023766011
prevalence and genetic diversity of pathogenic populations of vibrio parahaemolyticus in coastal waters of galicia, spain.the natural reservoirs and biological characteristics of pathogenic populations of vibrio parahaemolyticus in marine habitats remain unclear due to difficulties in obtaining pathogenic strains from the environment. the distribution and characteristics of pathogenic v. parahaemolyticus were investigated over 1 year in three coastal environments in galicia (spain), including areas of the major international ports in the region. vibrio parahaemolyticus was present in 35.3% of the samples analysed, ...201023765999
environmental occurrence and clinical impact of vibrio vulnificus and vibrio parahaemolyticus: a european perspective.vibrio vulnificus and vibrio parahaemolyticus are ubiquitous gram-negative bacterial pathogens found naturally in marine and estuarine waters, and are a leading cause of seafood-associated bacterial illness. these pathogens are commonly reported in the usa and in many asian countries, including china, japan and taiwan; however, there is growing concern that v. vulnificus and v. parahaemolyticus may represent an important and increasing clinical problem in europe. several factors underlie the nee ...201023765993
patient selection and results of cardiac transplantation in patients with cardiomyopathy.cardiac transplantation for the treatment of end-stage congestive heart failure has been shown to be of benefit regardless of the etiology. with few exceptions, the evaluation of patients with end-stage heart failure is the same, regardless of the etiology. in those with cardiomyopathy not as a result of cad, special attention must be given to exclude secondary causes of cardiomyopathy such as amyloidosis, hemochromatosis, and sarcoidosis, as well as generalized systemic illnesses that may also ...20103048684
patient selection and results of cardiac transplantation in patients with cardiomyopathy.cardiac transplantation for the treatment of end-stage congestive heart failure has been shown to be of benefit regardless of the etiology. with few exceptions, the evaluation of patients with end-stage heart failure is the same, regardless of the etiology. in those with cardiomyopathy not as a result of cad, special attention must be given to exclude secondary causes of cardiomyopathy such as amyloidosis, hemochromatosis, and sarcoidosis, as well as generalized systemic illnesses that may also ...20103048684
[value of abdominal pulsed duplex sonography].pulsed duplex sonography is a routine method for evaluation of superficial vessels. especially in the assessment of the extracranial vessels of the head and neck region duplex sonography proved to be the method of choice. modern technology allows the investigation of deep vessels by pulsed doppler sonography. this study tries to define the value of the combination of real time sonography and pulsed deep doppler sonography in the abdomen on the basis of the author's experiences. value and limitat ...20102954181
why not launch a new cadet nurse corps? 20103279779
[tuberous iododerma due to solutan]. 20102949449
endoscopic anatomy of choledochoduodenostomy in relation to clinical observations.thirty patients having undergone choledochoduodenostomy for benign biliary diseases were studied by means of endoscopy. the endoscopic anatomy of the anastomosis was classified into two types. inflammatory changes of the choledochal mucosa adjacent to the stoma were found in nine cases. endoscopy of the proximal and distal (blind) segment of the common bile duct was accomplished in 19 and nine patients, respectively. it is very probable that these inflammatory mucosal changes could lead to compl ...20103962952
[temporary minimal esophagostomy in the surgical treatment of malignant esophago-respiratory fistulas].oesophago-bronchial and oesophago-pleural fistulae are serious complications of oesophageal cancers. they occur earlier than is generally believed and, if untreated, result in death caused by septic and respiratory complications. an original two-stage surgical method is reported and illustrated by 3 cases. the first stage consists of excluding the oesophagus at both ends and introducing, through a small oesophagostomy, a foley's catheter to aspirate the secretions; the cardia is excluded by stap ...20102938171
elective testicular biopsy during chemotherapy for childhood leukaemia is of no clinical value.the clinical value of early detection of testicular leukaemia was assessed by elective wedge biopsy during the 12th or 18th month of therapy in children with acute lymphoblastic leukaemia. biopsy samples were taken in 106 of 238 consecutively treated boys who attained complete remission; only 1 had occult disease. samples were also taken in 2 boys with testicular leukaemia at diagnosis as soon as they achieved complete remission, and in 14 others at the end of therapy; none showed evidence of le ...20102863443
oxidation-reduction reactions of cytochrome b6 in a liposome-incorporated cytochrome b6-f complex.the chloroplast cytochrome b6-f complex, incorporated into phospholipid vesicles, shows proton translocation with an observed h+/e- ratio of approximately 2. the oxidation-reduction behavior of cytochrome b6 during electron transport from duroquinol to plastocyanin is affected by incorporation. the most obvious effect of incorporation is an increase in the duration of a steady-state level of cytochrome b6 that persists during electron transport. reagents that decrease activity increase the durat ...20102821919
evidence of antibacterial activities in peptide fractions originating from snow crab (chionoecetes opilio) by-products.antibacterial peptide fractions generated via proteolytic processing of snow crab by-products exhibited activity against gram-negative and gram-positive bacteria. among the bacterial strains tested, peptide fractions demonstrated inhibitory activity against the gram-negative bacteria such as aeromonas caviae, aeromonas hydrophila, campylobacter jejuni, listonella anguillarum, morganella morganii, shewanella putrefasciens, vibrio parahaemolyticus and vibrio vulnificus and against a few gram-posit ...201026781242
segregation analysis of juvenile myoclonic epilepsy.we examined the inheritance of juvenile myoclonic epilepsy (jme). we looked at both the trait of "epilepsy" and the trait of "epilepsy-plus-eeg abnormalities," since eeg abnormalities are frequently found in the clinically unaffected sibs of jme patients. we tested several modes of inheritance including the fully penetrant recessive and several two-locus models. we could reject all models tested (fully penetrant single-locus and two-locus models) when abnormal eegs were classified as "unaffected ...20103136050
possible role of insulin deficiency and growth hormone in severe retinopathy. 20102863521
[splenic cysts in childhood. presentation of 4 cases and review of the literature]. 20103041316
effects of blocked versus random practice by mentally retarded subjects on learning a novel skill.the purpose of this study was to assess the effects of different practice conditions on mentally retarded subjects learning a novel task. 20 subjects with a mean iq of 51.82 were randomly assigned to one of two practice conditions. the first group practiced at three different speeds (60, 30, and 15 rpm) on the pursuit rotor using a block arrangement in which each speed was practiced for five consecutive trials of 20 sec. duration before practicing at the next speed. the second group used random ...20102812988
effects of blocked versus random practice by mentally retarded subjects on learning a novel skill.the purpose of this study was to assess the effects of different practice conditions on mentally retarded subjects learning a novel task. 20 subjects with a mean iq of 51.82 were randomly assigned to one of two practice conditions. the first group practiced at three different speeds (60, 30, and 15 rpm) on the pursuit rotor using a block arrangement in which each speed was practiced for five consecutive trials of 20 sec. duration before practicing at the next speed. the second group used random ...20102812988
nutrition and brain function: trace elements. 20102856557
isolated duodenal tamponade for treatment of bleeding duodenal ulcer. 20102895833
biological markers of suicide risk and adolescent suicide. 20102905935
[diseases of the gastrointestinal tract and the development of stomach cancer].an analysis of 1,108 histories of outpatients with a follow-up of 10 years and longer (up to 40 years) was carried out in orekhovo-zuyevo (population--132,301). 554 patients had stomach cancer diagnosed in 1973-1978 while the other 554 selected at random were in control. the results are discussed in detail.20102938332
linkage disequilibrium, cystic fibrosis, and genetic counseling.strong linkage disequilibrium occurs between the cystic fibrosis (cf) locus and polymorphisms detected with the dna probes xv-2c and km-19. in a north american population, 86% of cf chromosomes occur with a haplotype which occurs on only 14% of normal chromosomes. an individual homozygous for the highest-risk haplotype has an 81-fold greater probability of carrying a cf allele than does an individual homozygous for the lowest-risk haplotype. the linkage-disequilibrium data can be used for prenat ...20102916578
hypomagnesemia in digitalized patients. 20103954538
porous acrylic cement: evaluation of a novel implant material.the preparation and some properties of in situ curing porous acrylic cement, a modification of the traditional bone cement, are described. through numerous animal experiments, the behavior of the porous cement in a biological environment has been studied. the histological evaluation reveals that hard and soft tissues readily grow into the pores of the cement, thus anchoring the implant to the host tissues. some inflammatory foci, decreasing in number with residence time, together with varying nu ...20103071237
porous acrylic cement: evaluation of a novel implant material.the preparation and some properties of in situ curing porous acrylic cement, a modification of the traditional bone cement, are described. through numerous animal experiments, the behavior of the porous cement in a biological environment has been studied. the histological evaluation reveals that hard and soft tissues readily grow into the pores of the cement, thus anchoring the implant to the host tissues. some inflammatory foci, decreasing in number with residence time, together with varying nu ...20103071237
fabric of many-colored threads. 20103056893
fabric of many-colored threads. 20103056893
screening for silent myocardial ischemia.since many patients do not experience angina prior to myocardial infarction or sudden death, screening of asymptomatic high-risk patients is necessary. exercise stress testing of asymptomatic patients with coronary risk factors can be performed by family physicians in their offices. for the physician trying to develop a rational screening policy for silent myocardial ischemia, the relatively low yield of positive test results creates a management dilemma.20103057838
screening for silent myocardial ischemia.since many patients do not experience angina prior to myocardial infarction or sudden death, screening of asymptomatic high-risk patients is necessary. exercise stress testing of asymptomatic patients with coronary risk factors can be performed by family physicians in their offices. for the physician trying to develop a rational screening policy for silent myocardial ischemia, the relatively low yield of positive test results creates a management dilemma.20103057838
[comparative efficacy of disinfectants on the poliovirus]. 20102999938
[chronobiology of dynamic equilibrium and recurrence of migraine crises].haemodynamic balance was assessed in relation to platelet-vessel homeostasis in the microcirculation in a group of 15 migraine sufferers and 15 healthy subjects. the results support the hypothesis of a relationship between the typical recurrence of migraine attacks and the chronobiological performance of the haemodynamic balance.20103736980
the adenosine triphosphate-pyrophosphate isotopic exchange reaction: a tool for determination of tryptophan.quantitative determination of tryptophan at the picomole level is described, using the atp-[32p]ppi isotopic exchange reaction catalyzed by tryptophanyl-trna synthetase. sensitivity limits of 500 fmol were obtained. the presence of other amino acids at a 1000-fold excess over tryptophan did not interfere significantly with the quantitative determination of tryptophan. the specificity of the reaction was checked using five tryptophan analogs. these analogs did not prevent the determination of try ...20103014918
malignant mixed mullerian tumor arising in extragenital endometriosis: report of a case and review of the literature.a primary malignant mixed mullerian tumor (mmmt) was found in the wall of the rectum of a 67-year-old female. the uterus, fallopian tubes, and ovaries had been previously excised for benign disease. the tumor was associated with foci of benign endometriosis. a literature review of extragenital mmmts indicates that these are extremely rare tumors. this case supports the theory that endometriosis may serve as a progenitor to extraendometrial malignancy.20103002920
peculiarities of the endocrine time structure in noninsulin-dependent adult-onset (type ii) diabetes mellitus.twenty noninsulin-dependent elderly diabetic patients, ten of whom were treated by oral hypoglycemic agents and ten of whom were regulated by diet alone, and 20 clinically healthy subjects matched for age, sex, height, and weight were examined with six blood and six urine samples at 4-hr intervals over a 24-hr span. plasma acth, cortisol, aldosterone, and dehydroepiandrosterone-sulfate (dhea-s) were determined by radioimmunoassay (ria); epinephrine, norepinephrine, and dopamine in urine were det ...20103037559
[mechanisms of cutaneous accidents related to non-steroidal anti-inflammatory agents]. 20102976215
combined thrombolytic therapy and coronary angioplasty for acute myocardial infarction.from september, 1983, to august, 1984, combined thrombolytic therapy and percutaneous transluminal coronary angioplasty was used to treat 22 cases of acute myocardial infarction. initial coronary angiograms showed total obstruction in 13 and severe stenosis in 9. intracoronary infusion of urokinase reopened 7 of 13 totally occluded lesions but left a residual severe stenosis. coronary angioplasty opened all of the remaining totally obstructed lesions and decreased the stenosis in 14 of 16 stenos ...20102939702
[esophageal diseases in the elderly]. 20103152439
interactions of monomeric igg bearing covalently bound c3b with polymorphonuclear leucocytes.the interactions of monomeric igg, monomeric c3b, dimeric c3b, and c3b covalently bound to igg (c3b-igg) with neutrophils were examined. c3b-igg heterodimers exhibited an increased affinity of binding to neutrophils, relative to c3b, both at half normal ionic strength (five-fold enhancement) and at normal ionic strength (six-fold enhancement). binding of monomeric igg to neutrophils was barely detectable and did not permit direct measurements of affinity in our assay conditions. the increased af ...20102953672
i.v. sedation for conservative dentistry. a comparison of midazolam and diazepam.midazolam and the emulsion formulation of diazepam were compared in a cross-over study in 50 patients undergoing out-patient conservative dentistry, with particular regard to sedation and the quality of recovery. both agents proved effective, but sedation was achieved more rapidly with midazolam (p = 0.001) and was more effective (p less than 0.02). significantly greater anterograde amnesia for the dental procedure (p less than 0.001) and a more rapid return to normal activities (p less than 0.0 ...20102937434
dice: nonclinical causes of overtreatment. 20102916481
dissociation-reconstitution experiments with nbd-modified f1: support for the presence of two catalytic beta-subunits. 20102901754
circadian rhythms: benzodiazepines set the clock. 20102871494
[use of the long muscle of the neck for eliminating disorders of velopharyngeal function]. 20102800187
the hypothalamic-pituitary-adrenal axis in depression. 20103035885
detection of vibrio cholerae and vibrio parahaemolyticus by molecular and culture based methods from source water to household container-stored water at the point-of-use in south african rural communities.detection methods for vibrio cholerae and vibrio parahaemolyticus which included the culture based approach with polymerase chain reaction (pcr) confirmation, pcr detection without enrichment and pcr with a pre-enrichment were developed and their performance evaluated. pcr assays targeted the sodb (v. cholerae species), flae (v. parahaemolyticus species), 16s rrna (vibrio and enterobacteriacea species) genes (multiplex 1) and v. cholerae o1 and v. cholerae o139 rfb genes, ctxa (cholera toxin) ge ...201020555205
[development and evaluation of whole-genome dna microarray of vibrio parahaemolyticus].we developed and evaluated a whole-genome dna microarray of vibrio parahaemolyticus.201020560361
[homology analysis of vibrio parahaemolyticus isolated by mpn method using pulsed-field gel electrophoresis and automated ribotyping].to study the molecular typing of 78 strains of vibrio parahaemolyticus isolated from guangdong by mpn method with pulsed-field gel electrophoresis (pfge) and automated ribotyping (rp).201020568474
tumor suppressor qm-like gene from disk abalone (haliotis discus discus): molecular characterization and transcriptional analysis upon immune challenge.we describe molecular characterization and transcriptional analysis of the gene encoding tumor suppressor qm-like protein, abqm, in the disk abalone haliotis discus discus. the full-length cdna (765-bp) of abqm was found to consist of a 654-bp orf coding for a 218 amino acid protein of a 25 kda molecular mass with a 10.2 isoelectric point. analysis of abqm sequence revealed the presence of characteristic motifs, including the ribosomal protein l10 signature, sh3-binding motif and two antibiotic ...201020580829
functional properties of proteins from the coelomic fluid of the wounded sea star asterias rubens (l).impact on viability and adhesion of three protein fractions, separated by size, from the coelomic fluid of wounded asterias rubens', was tested on autologous coelomocytes. in addition antimicrobial property of the protein fractions was tested on the gram-negative bacterium vibrio parahaemolyticus. all fractions promoted viability and the larger proteins facilitated adhesion of the coelomocytes. the strongest antimicrobial effect was caused by the fraction with the smallest proteins.201020600086
immunomagnetic separation of vibrio vulnificus with antiflagellar monoclonal antibody.raw oysters are primary vectors for vibrio vulnificus infections, and a rapid detection method for v. vulnificus in raw oysters before distribution would be an indispensable tool for the seafood industry. one approach to improving the recovery and detection of v. vulnificus without sacrificing assay time is through the use of immunomagnetic separation (ims). the aim of this study was to develop and optimize an ims protocol using anti-h (antiflagellar) antibody for determining the level of v. vul ...201020615341
quantitative risk assessment of vibrio parahaemolyticus in finfish: a model of raw horse mackerel consumption in japan.the aim of this study was to evaluate the effects of implemented control measures to reduce illness induced by vibrio parahaemolyticus (v. parahaemolyticus) in horse mackerel (trachurus japonicus), seafood that is commonly consumed raw in japan. on the basis of currently available experimental and survey data, we constructed a quantitative risk model of v. parahaemolyticus in horse mackerel from harvest to consumption. in particular, the following factors were evaluated: bacterial growth at all ...201020626688
stability and antimicrobial activity of allyl isothiocyanate during long-term storage in an oil-in-water emulsion.this study investigated the stability and antimicrobial activity of allyl isothiocyanate (aitc) in medium chain triglyceride (mct) or soybean oil (sbo) dispersed in an oil-in-water (o/w) system during long-term storage. oil type, content, and oxidative stability affect the stability and antimicrobial activity of aitc during storage. high oil content is favorable for aitc stability in the emulsion. notably, aitc with mct is more stable than aitc with sbo with the same oil content. consequently, a ...201020629866
clinical isolates of aeromonas veronii biovar veronii harbor a nonfunctional gene similar to the thermostable direct hemolysin-related hemolysin (trh) gene of vibrio parahaemolyticus.thermostable direct hemolysin-related hemolysin encoded by the trh gene is considered a major virulence factor in the pathogenesis of vibrio parahaemolyticus infections. in this study, we report the presence of a trh homolog in three clinical isolates of aeromonas veronii biovar veronii. the presence of a trh homolog in these strains of a. veronii was confirmed by pcr, followed by cloning, sequencing and colony hybridization using a digoxigenin-labelled probe. dna sequence analysis revealed that ...201020636974
antibacterial properties of essential oils and methanol extracts of sweet basil ocimum basilicum occurring in bangladesh.the antibacterial potential of essential oils and methanol extracts of sweet basil ocimum basilicum l. (lamiaceae) was evaluated for controlling the growth range of food-borne pathogenic bacteria. essential oils extracted by hydrodistillation from the leaves and stems were analyzed by gc-ms. fifty-seven compounds representing 94.9 and 96.1% of the total leaf and stem oils, respectively, were identified, of which methyl chavicol (36.7 and 29.9%), gitoxigenin (9.3 and 10.2%), trimethoquinol (10.3 ...201020645791
hfq regulates anti-oxidative ability in vibrio parahaemolyticus.hfq plays a fundamental role in bacterial cell physiology. it can stimulate or repress the expression of certain target genes, and there is a possibility that hfq regulates the oxidative stress response. however, how hfq functions that in vibrio parahaemolyticus remains speculative. in this paper, we explain the functions hfq plays in v. parahaemolyticus in the gene expression of superoxide dismutase gene and catalase gene, comparing the hfq deletion mutant strain to the parental strain. the res ...201020647674
involvement of cell shape and flagella in the bacterial retention during percolation of contaminated water through soil columns in tropical region.microorganisms' retention in soil contributes to the natural purification of groundwater. bacteria found in groundwater are generally of various shapes. the aim of this study was to assess the importance of cell shape and flagella in bacterial retention during polluted water percolation through two soil columns ca and cb, in the equatorial region in central africa. percolation tests were carried out using different water loads samples which were contaminated by escherichia coli (straight rods, p ...201020658409
a highly sensitive and specific multiplex pcr assay for simultaneous detection of vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus.to develop an effective multiplex pcr for simultaneous and rapid detection of vibrio cholerae, vibrio vulnificus and vibrio parahaemolyticus, the three most important vibrio species that can cause devastating health hazards among human.201020666989
[cdna cloning, characterization and mrna expression of a profilin from the swimming crab portunus trituberculatus].we isolated and characterized the profilin full-length cdna from hemocytes of swimming crab portunus trituberculatus. the profilin cdna consists of 742 bp and the 375 bp open reading frame encodes a polypeptide of 125 amino acids, having a predicted isoelectric point of 5.87. the deduced amino acid sequence shows 42.9% amino acid sequence identity to the profilin of mosquito anopheles gambiae. the profilin mrna was highly expressed in hemocytes and moderately in hepatopancreas of normal crab. th ...201020672413
comparative genomic analyses identify the vibrio harveyi genome sequenced strains baa-1116 and hy01 as vibrio campbellii.three notable members of the harveyi clade, vibrio harveyi, vibrio alginolyticus and vibrio parahaemolyticus, are best known as marine pathogens of commercial and medical import. in spite of this fact, the discrimination of harveyi clade members remains difficult due to genetic and phenotypic similarities, and this has led to misidentifications and inaccurate estimations of a species' involvement in certain environments. to begin to understand the underlying genetics that complicate species leve ...201020686623
brichos domain-containing leukocyte cell-derived chemotaxin 1-like cdna from disk abalone haliotis discus discus.a brichos domain-containing leukocyte cell-derived chemotaxin 1-like cdna was cloned from the disk abalone (haliotis discus discus) and designated as ablect-1. a full-length (705 bp) of ablect-1 cdna was composed of a 576 bp open reading frame that translates into a putative peptide of 192 amino acids. deduced amino acid sequence of ablect-1 had 15.5- and 27.8% identity and similarity to human lect-1, respectively. quantitative real-time pcr analysis results showed that the mrna of ablect-1 was ...201020688173
detection and differentiation of vibrio spp. in seafood and fish samples with cultural and molecular methods.vibrio spp. as natural inhabitants of sea- and brackwater of both tropical and temperate regions of the world are commonly found in different kinds of seafood. even among the three main human pathogenic species vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus most of the isolates from seafood do not carry the different virulence factors responsible for foodborne infections. therefore, the risk assessment of vibrio spp. in seafood is currently based mainly on the knowledge of the ge ...201020688407
characterization of myosin light chain in shrimp hemocytic phagocytosis.myosin light chain, a well-known cytoskeleton gene, regulates multiple processes that are involved in material transport, muscle shrink and cell division. however, its function in phagocytosis against invading pathogens in crustacean remains unknown. in this investigation, a myosin light chain gene was obtained from marsupenaeus japonicus shrimp. the full-length cdna of this gene was of 766 bp and an open reading frame (orf) of 462 bp encoding a polypeptide of 153 amino acids. the myosin light c ...201020691789
isolation, identification and molecular characterization of vibrio parahaemolyticus from fish samples in kolkata.vibrio parahaemolyticus is a marine bacterium which is also responsible for acute diarrhoeal illness in human beings. eating raw seafish or contaminated seafood is responsible for acute gastroenteritis. the aim of this study was to investigate the isolation, identification and molecular characterization of vibrio parahaemolyticus from the fish samples in kolkata, india.201020712262
transcription of vibrio parahaemolyticus t3ss1 genes is regulated by a dual regulation system consisting of the exsacde regulatory cascade and h-ns.vibrio parahaemolyticus, one of the human pathogenic vibrios, causes gastroenteritis, wound infections and septicemia. genomic sequencing of this organism revealed that it has two distinct type iii secretion systems (t3ss1 and t3ss2). t3ss1 plays a significant role in lethal activity in a murine infection model. it was reported that expression of the t3ss1 gene is controlled by a positive regulator, exsa, and a negative regulator, exsd, which share a degree of sequence similarity with pseudomona ...201020722736
a vibrio effector protein is an inositol phosphatase and disrupts host cell membrane integrity.the marine bacterium vibrio parahaemolyticus causes gastroenteritis in humans and encodes the type iii effector protein vpa0450, which contributes to host cell death caused by autophagy, cell rounding, and cell lysis. we found that vpa0450 is an inositol polyphosphate 5-phosphatase that hydrolyzed the d5 phosphate from the plasma membrane phospholipid phosphatidylinositol 4,5-bisphosphate. vpa0450 disrupted cytoskeletal binding sites on the inner surface of membranes of human cells and caused pl ...201020724587
first molluscan transcription factor activator protein-1 (ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury.the regulation of transcriptional activation is an essential and critical point in gene expression. in this study, we describe a novel transcription factor activator protein-1 (ap-1) gene from disk abalone haliotis discus discus (abap-1) for the first time in mollusk. it was identified by homology screening of an abalone normalized cdna library. the cloned abap-1 consists of a 945 bp coding region that encodes a putative protein containing 315 amino acids. the abap-1 gene is composed of a charac ...201020732431
origins and colonization history of pandemic vibrio parahaemolyticus in south america.the dynamics of dissemination of the environmental human pathogen vibrio parahaemolyticus are uncertain. the o3:k6 clone was restricted to asia until its detection along the peruvian coasts and in northern chile in 1997 in phase with the arrival of el niño waters. a subsequent emergence of o3:k6 strains was detected in austral chile in 2004. the origin of these 1997 and 2004 population radiations has not yet been conclusively determined. multiple loci vntr analysis using seven polymorphic loci w ...201020735744
structure of a longitudinal actin dimer assembled by tandem w domains: implications for actin filament nucleation.actin filament nucleators initiate polymerization in cells in a regulated manner. a common architecture among these molecules consists of tandem wasp homology 2 domains (w domains) that recruit three to four actin subunits to form a polymerization nucleus. we describe a low-resolution crystal structure of an actin dimer assembled by tandem w domains, where the first w domain is cross-linked to cys374 of the actin subunit bound to it, whereas the last w domain is followed by the c-terminal pointe ...201020804767
relationships between environmental factors and pathogenic vibrios in the northern gulf of mexico.although autochthonous vibrio densities are known to be influenced by water temperature and salinity, little is understood about other environmental factors associated with their abundance and distribution. densities of culturable vibrio vulnificus containing vvh (v. vulnificus hemolysin gene) and v. parahaemolyticus containing tlh (thermolabile hemolysin gene, ubiquitous in v. parahaemolyticus), tdh (thermostable direct hemolysin gene, v. parahaemolyticus pathogenicity factor), and trh (tdh-rel ...201020817802
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