Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| on the significance of the prosthetic group composition of citrate lyase. | 1. klebsiella aerogenes contains two different acyl carrier proteins, one specific for citrate lyase, the other for fatty acid synthetase. 2. the acyl carrier protein of fatty acid synthetase from k. aerogenes was isolated and compared with the corresponding protein from escherichia coli and with the acyl carrier protein of citrate lyase from k. aerogenes. 3. as judged from prosthetic group compositions as well as amino acid and fingerprint analyses, the acyl carrier proteins of the two fatty ac ... | 1978 | 350581 |
| selective inhibition of klebsiella aerogenes growth on pentoses by pentitols. | selective inhibition of growth by pentitols was observed when klebsiella aerogenes m-7 which could not utilize pentitols was grown on pentoses. d-arabitol inhibited the growth on d-arabinose as a sole carbon source, but had no effect on the growth on l-arabinose, d-xylose, and d-ribose. similarly, l-arabitol inhibited the growth on d-arabinose and l-arabinose, ribitol inhibited the growth on d-arabinose and l-arabinose, and xylitol inhibited the growth on d-xylose. from the following reasons, we ... | 1978 | 350845 |
| [severe klebsiella serogenes infections]. | it is reported on 2 cases of klebsiella-septikaemia with difficult course. germs of the group klebsiella aerogenes could be proved microbiologically. on the basis of the peculiarities of the clinical course the author adopts a definite attitude to the development of the infection, the pathogenesis, to the microbiological proof and to the aimed antimicrobial chemotherapy. the antibiotic treatment was successful in the first case with karbenicillin and gentamycin and in the second case with cephal ... | 1978 | 351992 |
| amino-acid sequence of citrate-lyase acyl-carrier protein from klebsiella aerogenes. | 1978 | 352686 | |
| differentiation of serratia marcescens and serratia liquefaciens by tests for lipase and phospholipase production. | the production of lipase and phospolipase by certain members of the enterobacteriaceae was examined by thin-layer chromatography of resting-cell suspensions incubated with triolein or lecithin. most strains of serratia marcescens produced both enzymes while most strains of serratia liquefaciens exhibited strong lipase but only a minor phospholipase activity. enterobacter spp. (25 strains), klebsiella pneumoniae (20 strains), escherichia coli (15 strains), citrobacter freundii (7 strains) and pro ... | 1978 | 355631 |
| trimethoprim-resistant klebsiella aerogenes. | 1978 | 78356 | |
| effect of ph on the antimicrobial activity of some triphenylmethane dyes. | four common dyes were tested as inhibitors of four types of bacteria over the ph range 5.0-9.0. inhibition of the gram-negative types, salmonella anatum and enterobacter aerogenes, was markedly affected by the ph of the medium. these organisms tolerated concentrations of crystal violet and ethyl violet about 100-fold higher at ph 5.0 than at ph 9.0. above ph 7.0 brilliant green (bg) and malachite green (mg) were precipitated as their respective carbinols and lost their inhibitory properties with ... | 1978 | 27297 |
| electron paramagnetic resonance studies on membrane-bound respiratory nitrate reductase of klebsiella aerogenes. | 1978 | 207577 | |
| coenzyme b12-dependent diol dehydratase: regulation of apoenzyme synthesis in klebsiella pneumoniae (aerobacter aerogenes) atcc 8724. | immunochemical studies demonstrated that klebsiella pneumoniae (aerobacter aerogenes) atcc 8724 produces only a single diol dehydratase whether grown on glycerol or on 1,2-propanediol. the enzyme was subject to induction by 1,2-diols and to catabolite repression reversed by cyclic amp. | 1978 | 210157 |
| aminopeptidase i activities in several microorganisms. | aminopeptidase i activity which was found to be localized in the same subcellular fraction and to be similarly heat stable was partially purified by a common procedure from escherichia coli b, escherichia coli k12, enterobacter aerogenes, salmonella typhimurium, serratia marcescens pseudomonas aeruginosa, and proteus vulgaris. the enzyme preparations were shown to contain a single animopeptidase active toward both leucylleucine and methionylalanylserine by mixed-substrate initial-velocity kineti ... | 1978 | 110425 |
| beta-lactam antibiotics. ii. structure-activity relationships of 6-[alpha-(alpha'-ureido-acylamino) acylamino] penicillanic acids. | the influence on the structure-activity relationships (s.a.r.) of the stereochemistry and various alkyl, aryl, aralkyl and heterocyclic substituents at the two chiral centres in the dipeptide side-chain of a new series of penicillins was examined. in many cases the effects of these changes had a pronounced influence on the degree of activity against gram-positive and especially gram-negative bacteria. several compounds indicated that the size, shape and spatial disposition of a substituent were ... | 1978 | 101499 |
| catabolic n2-acetylornithine 5-aminotransferase of klebsiella aerogenes: control of synthesis by induction, catabolite repression, and activation by glutamine synthetase. | klebsiella aerogenes formed two n2-acetylornithine 5-aminotransferases (acoat) which were separable by diethylaminoethyl-cellulose chromatography. one acoat was repressed when the cells grew on arginine-containing medium, indicating its function in arginine biosynthesis. the second acoat was induced when arginine or ornithine was present in the medium as the sole source of carbon or nitrogen, suggesting its function in the catabolism of these compounds. the induced enzyme was purified almost to ... | 1978 | 24039 |
| bacterial 2,3-butanediol dehydrogenases. | enterobacter aerogenes, aeromonas hydrophila, serratia marcescens and staphylococcus aureus possessing l(+)-butanediol dehydrogenase produced mainly meso-butanediol and small amounts of optically active butanediol; acetobacter suboxydans, bacillus polymyxa and erwinia carotovora containing d(-)-butanediol dehydrogenase produced more optically active butanediol than meso-butanediol. resting and growing cells of these organisms oxidezed only one enantiomer of racemic butanediol. the d(-)-butanedio ... | 1978 | 25056 |
| involvement of the product of the glnf gene in the autogenous regulation of glutamine synthetase formation in klebsiella aerogenes. | mutations in a site, glnf, linked by p1-mediated transduction of argg on the chromosome of klebsiella aerogenes, result in a requirement for glutamine. mutants in this gene have in all media a level of glutamine synthetase (gs) corresponding to the level found in the wild-type strain grown in the medium producing the strongest repression of gs. the adenylylation and deadenylylation of gs in glnf mutants is normal. the glutamine requirement of glnf mutants could be suppressed by mutations in the ... | 1978 | 25264 |
| glutamine synthetase of klebsiella aerogenes: properties of glnd mutants lacking uridylyltransferase. | the glnd mutation of klebsiella aerogenes is cotransducible by phage p1 with pan (requirement for pantothenate) and leads to a loss of uridylytransferase and uridylyl-removing enzyme, components of the glutamine synthetase adenylylation system. this defect results in an inability to deadenylylate glutamine synthetase rapidly and in a requirement for glutamine for normal growth. suppression of the glnd mutation are located at the glutamine synthetase structural gene glna. | 1978 | 26659 |
| [effect of synthetic iron chelating agensts and of a source of carbohydrate nutrition on catechol biosynthesis by klebsiella aerogenes]. | 1978 | 416325 | |
| trypsin peptide patterns of tryptophan synthase beta2 protein among four species of the enterobacteriaceae. | the tryptophan synthase beta 2 protein (ec 4.2.1.20) of escherichia coli, enterobacter aerogenes, serratia marcescens, and erwinia carotovora was purified and compared. two-dimensional total peptide patterns for each of the four beta2 proteins obtained after digestion with trypsin showed that approximately three quarters of the total peptides are common to all four peptides. examination of only arginine-containing peptides showed that approximately half of these peptides are common. from a compa ... | 1978 | 361723 |
| phosphoribosylglycinamide synthetase from aerobacter aerogenes. | 1978 | 692385 | |
| a sulphate metabolite of trimethoprim in goats and pigs. | 1. urine from goats and pigs after intravenous administration of [14c]trimethoprim contained an unidentified fraction of 'other water-soluble metabolites'. this fraction accounts for 56% of the administered dose in goat urine and 65% in pig urine. 2. a sulphate metabolite comprising the major part of the latter fraction in both goat and pig urine was isolated by gel filtration and paper chromatography and identified by spectroscopic methods as 2,4-diamino-5-(3,4-dimethoxy-5-sulphooxybenzyl)pyrim ... | 1978 | 726512 |
| the effects of temperature and growth rate on the proportion of unsaturated fatty acids in bacterial lipids. | the effects of temperature and growth rate on the fatty acid composition of the extractable lipids of four mesophilic and three psychotrophic bacteria were examined. two of the mesophiles (escherichia coli and pseudomonas aeruginosa) increased the proportion of unsaturated fatty acids in their lipids with decreasing temperature over their whole growth temperature range. the other mesophiles (enterobacter aerogenes and lactobacillus casei) increased the proportion of unsaturated fatty acids with ... | 1978 | 624935 |
| carbon and nitrogen nutrition of aerobacter aerogenes for valine production. | a number of carbon and nitrogen compounds were tested for their effect on growth of aerobacter aerogenes and valine production. sucrose was found to be the superior carbon source for valine production, while mannose supported maximum cellular growth with very poor yield of valine. urea was found to be the adequate nitrogen source for valine production. supplementation of the synthetic medium with complex nutrients lowered the yield of valine. no direct relationship between the growth of the orga ... | 1978 | 749410 |
| enterobacter aerogenes primary bacteremia in pediatric patients. | enterobacter aerogenes bacteremia associated with the infusion of contaminated admixed intravenous (iv) fluid occurred in seven patients in a pediatric hospital over a five-day period. clinical illness was characterized by spiking fever in all patients. the temporal clustering of cases allowed for rapid recognition of the problem. the primary control measure was the prompt replacement of the iv fluids, although iv antibiotics were also administered. hospital pharmacy practices for admixing iv so ... | 1978 | 704200 |
| method for the simultaneous establishment of many axenic cultures of paramecium. | a method is described for the simultaneous treatment of 42 (or more) stocks of paramecium, and their adaptation to growth in axenic culture. samples of dense cultures of these ciliates growing with enterobacter aerogenes are rendered bacteria-free by migration through 2 sets of tubes containing adaptation medium (peters' salts solution, stigmaterol, vitamins, and autoclaved e. aerogenes). the 2nd set of tubes contains adaptation medium plus antibiotics. bacteria-free samples containing approxima ... | 1978 | 660565 |
| 5-methylthioribose kinase. a new enzyme involved in the formation of methionine from 5-methylthioribose. | the presence of a previously unidentified enzyme, tentatively designated 5-methylthioribose kinase, has been demonstrated in cell-free extracts of enterobacter aerogenes. the enzyme catalyzes the atp-dependent phosphorylation of 5-methylthioribose. adp is one of the products of the reaction and, based on functional group analyses, the other product is 5-methylthioribose 1-phosphate. a 40-fold purified enzyme preparation has been obtained from a cell-free extract of e. aerogenes. activity of the ... | 1978 | 210167 |
| phenotypic modifications in amino acid profiles of cell residues of candida utilis and enterobacter aerogenes. | glucose-limited chemostat cultures of candida utilis were cultivated at various ph levels (3.0-7.5), temperatures (15-37.5 degrees c), dilution rates (0.006-0.42 hr-1), and with one of two nitrogen sources (nh+4 or no-3). enterobacter aerogenes was also cultivated in the chemostat under nitrogen and phosphorus limitations. the amino acid profile of total cell protein is expressed as the content of each amino acid relative to the sum of all amino acids recovered ater acid hydrolysis. cell residue ... | 1977 | 560224 |
| respiratory infection in an intensive care unit. | a postmortem bacteriological study of black children in a respiratory intensive care unit showed that pseudomonas aeruginosa was the most common opportunistic pathogen and that it usually complicated a viral infection. in a parallel study of non-debilitated patients in general hospital wards klebsiella aerogenes and escherichia coli were the most frequently isolated organisms. counter-immuno-electrophoresis was used for the identification of pseudomonas-precipitating antibody in serum and trache ... | 1977 | 416503 |
| [catechol biosynthesis by klebsiella aerogenes bacteria]. | 1977 | 865322 | |
| clavulanic acid: a beta-lactamase-inhiting beta-lactam from streptomyces clavuligerus. | a novel beta-lactamase inhibitor has been isolated from streptomyces clavuligerus atcc 27064 and given the name clavulanic acid. conditions for the cultivation of the organism and detection and isolation of clavulanic acid are described. this compound resembles the nucleus of a penicillin but differs in having no acylamino side chain, having oxygen instead of sulfur, and containing a beta-hydroxyethylidine substituent in the oxazolidine ring. clavulanic acid is a potent inhibitor of many beta-la ... | 1977 | 879738 |
| specific gonadotropin binding to pseudomonas maltophilia. | binding of 125i-labeled human chorionic gonadotropin to pseudomonas maltophilia is dependent on time, temperature, and ph and the binding to this procaryotic species is hormone-specific and saturable. the equilibrium dissociation constant is 2.3 x 10(-9) m. there are no cooperative interactions between binding sites (hill coefficient, 1.05). the number of sites is estimaated as 240 fmol/100 mug of protein. nacl and kcl, at concentrations from 1 to 10 mm, have no effect on binding. divalent catio ... | 1977 | 265583 |
| excystment of axenically prepared cysts of hartmanella culbertsoni. | axenically prepared cysts of hartmannella culbertsoni readily excysted in the presence of heat stable factors prepared from escherichia coli, klebsiella aerogenes, staphylococcus aureus, sarcina lutea, bacillus subtilis, bacillus megaterium and several fungi. peptone, proteose peptone, tryptone or amino acids also promoted excystment. crowding of the cysts and dilution of bacterial extracts adversely affected the excystment. continual presence of the factors in the medium was essential for excys ... | 1977 | 319194 |
| taxonomy of the genus serratia. | one hundred and fifty-six strains of serratia and related bacteria including representatives of enterobacter liquefaciens, enterobacter cloacae, enterobacter aerogenes, erwinia carotovora, erwinia chrysanthemi, erwinia herbicola and erwinia nimipressuralis were studied using 223 morphological, physiological, biochemical and carbon source utilization tests. the results were subjected to computer analysis. at the 80% similarity level all strains, except two, grouped into eight phenons representing ... | 1977 | 319202 |
| in vitro activities of five oral cephalosporins against aerobic pathogenic bacteria. | cefaclor (lilly 99638) and cefatrizine (bl-s640, sk&f 70771) are orally absorbed, broad-spectrum semisynthetic cephalosporins. they were compared in vitro with cephalexin, cephaloglycin, and cepharadine against a variety of aerobic pathogenic bacteria by an agar dilution procedure. cefaclor and cefatrizine were found to be similar or superior to cephalexin, cephaloglycin, and cephradine in terms of activity against gram-positive cocci other than enterococci. only cefatrizine demonstrated any pot ... | 1977 | 921258 |
| intracellular crystalline deposits by bacteria grown in urine from a stone former. | several species of bacteria were found to form an intracellular crystalline material when grown in urine obtained from a subject with a history of infrequent renal calculi formation. the following species: proteus mirabilis, proteus rettgeri, providencia stuartii, enterobacter aerogenes, enterobacter cloacae, escherichia coli, and candida albicans formed crystals of hydroxyapatite. klebsiella pneumoniae, pseudomonas aeruginosa, and proteus vulgaris produced crystals of calcite -ii. several of th ... | 1977 | 844994 |
| [9-(adenylyl)alkylcobalamins as inhibitors of adenosylcobalamin-dependent glycerol dehydratase from aerobacter aerogenes]. | the behavior of two coenzyme analogs, [(5-aden-9-yl)methoxyethyl] cob (iii) alamin and [(5-aden-9-yl)pentyl] cob (iii) alamin modified at the nucleoside ligand sugar moiety was studied in the system of adenosyl-cobalamin-dependent glycerol dehydratase from aerobacter aerogenes. it was shown that neither of the analogs possesses coenzyme properties and that both are strong competitive inhibitors for adenosylcobalamin (adocbl). the affinity of the two analogs for the apoenzyme is higher than that ... | 1977 | 588639 |
| tyramine oxidase and regulation of arylsulfatase synthesis in klebsiella aerogenes. | the participation of tyramine oxidase in the regulation of arylsulfatase synthesis in klebsiella aerogenes was studied. arylsulfatase was synthesized when this organism was grown with methionine or taurine as the sulfur source (nonrepressing conditions) and was repressed by inorganic sulfate or cysteine; this repression was relieved by tyramine and related compounds (derepressing conditions). under nonrepressing conditions, arylsulfatase synthesis was not regulated by tyramine oxidase synthesis. ... | 1977 | 830648 |
| genetic control of arylsulfatase synthesis in klebsiella aerogenes. | it was shown that at least four genes are specifically responsible for arylsulfatase synthesis in klebsiella aerogenes. mutations at chromosome site atsa result in enzymatically inactive arylsulfatase. mutants showing constitutive synthesis of arylsulfatase (atsr) were isolated by using inorganic sulfate or cysteine as the sulfur source. another mutation in which repression of arylsulfatase by inorganic sulfate or cysteine could not be relieved by tyramine was determined by genetic analysis to b ... | 1977 | 853036 |
| growth of klebsiella aerogenes on xylitol: implications for bacterial enzyme evolution. | when klebsiella aerogenes was grown in continuous culture with xylitol. an unnatural pentitol, as the growth limiting substrate, the structural gene which codes for ribitol dehydrogenase, an enzyme which gratuitously catalyzes the oxidation of xylitol to d-xylulose, was duplicated. it appears that the duplication mechansim only duplicates the gene which is subjected to selective pressure and not any of the other closely linked genes. the degree to which the ribitol dehydrogenase gene is duplicat ... | 1977 | 864722 |
| the utilization of the plasticizer dimethyl phthalate by an isolated strain of enterobacter aerogenes. | 1977 | 884330 | |
| numerical taxonomy and ecology of petroleum-degrading bacteria. | a total of 99 strains of petroleum-degrading bacteria isolated from chesapeake bay water and sediment were identified by using numerical taxonomy procedures. the isolates, together with 33 reference cultures, were examined for 48 biochemical, cultural, morphological, and physiological characters. the data were analyzed by computer, using both the simple matching and the jaccard coefficients. clustering was achieved by the unweighted average linkage method. from the sorted similarity matrix and d ... | 1977 | 889329 |
| study on the mechanism of action of adenosylcobalamin-dependent glycerol dehydratase from aerobacter aerogenes. i. role of structural components of adenosylcobalamin the formation of the active site of glycerol dehydratase. | a new method of partial chemical synthesis of adenosylcobalamin (co alpha-[alpha-5,6-diemethylbenzimidazolyl)]-co beta-adenosylcobamide, adocbl) analogs has been developed. a series of derivatives of adocbl modified in the nucleoside and nucleotide ligands and corrin macrocycle have been obtained. the interaction of adocl analogs with glycerol dehydratase (ec 4.2.1.30) from aerobacter aerogenes has been investigated. it has been shown that the nucleoside ligand of adocbl provides no essential co ... | 1977 | 889845 |
| study of the mechanism of action of adenosylcobalamindependent glycerol dehydratase from aerobacter aerogenes. ii. the inactivation kinetics of glycerol dehydratase complexes with adenosylobalamin and its analogs. | the inactivation kinetics of vacterial glycerol dehydratase (ec 4.2.1.30) in the course of its reaction with adenosylcobalamin (adocbl) and its analogs were investigated. it was shown that the inactivation rate of apoenzyme complexes with adocbl analogs is determined by the nature of the analogs employed and probably by the rate of their conversion into hydroxycobalamins. a possible inactivation mechanism of glycerol dehydratase is discussed. | 1977 | 889846 |
| enzymatic and immunological characterization of a new cephalosporinase from enterobacter aerogenes. | a hospital strain of enterobacter aerogenes (mulb 250) isolated from a urinary tract infection was found to be cephalosporin and ampicillin resistant and carbenicillin susceptible. the beta-lactamase produced by this strain was extracted and purified by means of affinity chromatography, using a cephalosporin c-bound sepharose 4b column. the purified enzyme was tested for hydrolysis of penicillin and various cephalosporins. the k(m) value is 11.8 mum for benzyl penicillin and 130 mum for cephalos ... | 1977 | 900918 |
| purification and properties of citrate lyase ligase from streptococcus diacetilactis. | citrate lyase ligase (acetate: sh--[acyl-carrier protein] enzyme ligase (amp) from streptococcus diacetilactis was purified 920-fold with a yield of 6.3%. the molecular weight of the enzyme was estimated to be 41000; the ligase consisted of one polypeptide chain. the acetylation of 1 mol of deacetyl-citrate lyase to enzymatically active citrate lyase required 6 mol atp. the formation of amp and pyrophosphate in the acetylation reaction was demonstrated. citrate lyase ligase was specific for the ... | 1977 | 923578 |
| energy-dependent inactivation of citrate lyase in enterobacter aerogenes. | enterobacter aerogenes was grown in continous culture with ammonia as the growth-limiting substrate, and changes in citrate lyase and citrate synthase activities were monitored after growth shifts from anaerobic growth on citrate to aerobic growth on citrate, aerobic growth on glucose, anaerobic growth on glucose, and anaerobic growth on glucose plus nitrate. citrate lyase was inactivated during aerobic growth on glucose and during anaerobic growth with glucose plus nitrate. inactivation did not ... | 1977 | 924971 |
| immunological study of the regulation of cellular arylsulfatase synthesis in klebsiella aerogenes. | regulation of cellular arylsulfatase synthesis in klebsiella aerogenes was analyzed by immunological techniques. antibody directed against the purified arylsulfatase from k. aerogenes w70 was obtained from rabbits and characterized by immunoelectrophoresis, double-diffusion, quantitative precipitation, and enzyme neutralization tests. arylsulfatase was located in the periplasmic space when the wild-type strain was cultured with methionine or with inorganic sulfate plus tyramine, but not with ino ... | 1977 | 72063 |
| biochemical parameters of glutamine synthetase from klebsiella aerogenes. | the glutamine synthetase (gs) from klebsiella aerogenes is similar to that from escherichia coli in several respects: (i) it is repressed by high levels of ammonia in the growth medium; (ii) its biosynthetic activity is greatly reduced by adenylylation; and (iii) adenylylation lowers the ph optimum and alters the response of the enzymes to various inhibitors in the gamma-glutamyl transferase (gammagt) assay. there are, however, several important differences: (i) the isoactivity point for the ade ... | 1977 | 14104 |
| regulation of glna messinger ribonucleic acid synthesis in klebsiella aerogenes. | we examined wild-type and mutant strains of klebsiella aerogenes for the relative amounts of ribonucleic acid (rna) hybridizing specifically to deoxyribonucleic acid from a transducing phage carrying glnak, the structural gene for glutamine synthetase. our data showed a positive correlation between the intracellular level of glutamine synthetase and the level of glna messenger rna; we were unable to detect glna messinger rna in strains devoid of glutamine synthetase protein. therefore, it is pos ... | 1977 | 14114 |
| glutamine synthetase of klebsiella aerogenes: genetic and physiological properties of mutants in the adenylylation system. | mutations resulting in defects in the adenylylation system of glutamine synthetase (gs) affect the expression of glna, the structural gene for gs. mutants with lesions in glnb are glutamine auxotrophs and contain repressed levels of highly adenylylated gs. glutamine-independent revertants of the glnb3 mutant have acquired an additional mutation at the glne site. the glne54 mutant is incapable of adenylylating gs and produces high levels of enzyme, even when ammonia is present in the growth mediu ... | 1977 | 14117 |
| lack of a regulatory function for glutamine synthetase protein in the synthesis of glutamate dehydrogenase and nitrite reductase in escherichia coli k12. | synthesis of glutamine synthetase (gs) in anaerobic batch cultures of escherichia coli was repressed when excess nh4+ was available, but derepressed during growth with a poor nitrogen source. in wild-type bacteria there was only a weak inverse correlation between the activities of gs and glutamate dehydrogenase (gdh) during growth in various media. no positive correlations were found between the activities of gs and nitrite reductase, or between gs and cytochrome c552: both of these proteins wer ... | 1977 | 16079 |
| regulation of enzyme synthesis by the glutamine synthetase of salmonella typhimurium: a factor in addition to glutamine synthetase is required for activation of enzyme formation. | in klebsiella aerogenes but not in salmonella typhimurium glutamine synthetase can function during nitrogen-limited growth to increase the rate of synthesis of histidase from the hut genes of s. typhimurium 15-59 (huts. 15-59). formation of proline oxidase is also not increased in nitrogen-limited cultures of s. typhimurium. however, in hybrid strains of escherichia coli or k. aerogenes, the glutamine synthetase of s. typhimurium activates synthesis of histidase from the huts. 15-59 genes. appar ... | 1977 | 16868 |
| transport of cyclitols by a proton symport in klebsiella aerogenes. | the respiration and the atp content of klebsiella aerogenes in the presence of various inhibitors were compared to the transport of scyllo-inositol. the atpase was found to be inhibited by dicyclohexyl carbodiimide. the transport has been tested in anaerobiosis and aerobiosis. from the results obtained it is concluded that either atp or respiration can sustain the transport activity in independent manner. 2. the energy derived from the respiratory chain reactions or the atp hydrolysis results in ... | 1977 | 12979 |
| urease of klebsiella aerogenes: control of its synthesis by glutamine synthetase. | urease was purified 24-fold from extracts of klebsiella aerogenes. the enzyme has a molecular weight of 230,000 as determined by gel filtration, is highly substrate specific, and has a km for urea of 0.7 mm. a mutant strain lacking urease was isolated; it failed to grow with urea as the sole source of nitrogen but did grow on media containing other nitrogen sources such as ammonia, histidine, or arginine. urease was present at a high level when the cells were starved for nitrogen; its synthesis ... | 1977 | 18438 |
| regulatory mutations in the klebsiella aerogenes structural gene for glutamine synthetase. | glutamine synthetase could be repressed several hundredfold rather than 6- to 10-fold as previously reported. ammonia was not the primary repression signal for glutamine synthetase. repression appeared to be mediated by a high level of glutamine and probably by a high ratio of glutamine to alpha-ketoglutarate. mutations in glna (the structural gene for glutamine synthetase) were seen to fall into three phenotypic groups: glutamine auxotrophs that produced no detectable glna product; glutamine au ... | 1977 | 21157 |
| autogenous regulation of the synthesis of glutamine synthetase in klebsiella aerogenes. | we isolated an f' episome of escherichia coli carrying the glna+ gene from k. aerogenes and an f' episome of e. coli carrying the glna4 allele from k. aerogenes responsible for the constitutive synthesis of glutamine synthetase. complementation tests with these episomes showed that the glna4 mutation (leading to the constitutive synthesis of active glutamine synthetase) was in the gene identified by mutations glna20, glna51, and glna5 as the structural gene for glutamine synthetase. by using the ... | 1977 | 21158 |
| purification and initial rate kinetics of acyl-phosphate-hexose phosphotransferase from aerobacter aerogenes. | the enzyme acyl-phosphate-hexose phosphotransferase from aerobacter aerogenes was purified to electrophoretic homogeneity. the molecular weight of the enzyme as determined on sephadex gels is 150 000. the enzyme possesses potent phosphotransferase and phosphohydrolase activities. initial rate kinetics were used to investigate the mechanism of acyl-phosphate-hexose phosphotransferase. these studies, which involved a number of different phosphoryl donors and substrate analogues, suggest that the k ... | 1977 | 196625 |
| intracellular distribution of enzymes of phospholipid metabolism in several gram-negative bacteria. | cell-free extracts of salmonella typhimurium, serratia marcescens, enterobacter aerogenes, and micrococcus cerificans contained the following enzymatic activities related to phospholipid metabolism: cytidine 5'-diphospho-1,2-diacyl-sn-glycerol (cdp-diglyceride):l-serine o-phosphatidyltransferase (phosphatidylserine synthase), phosphatidylserine decarboxylase, cdp-diglyceride:sn-glycero-3-phosphate phosphatidyltransferase (phosphatidylglycerophosphate synthase), phosphatidylglycerophosphate phosp ... | 1977 | 199573 |
| effect of metabolites on epsilon-n-hydroxylysine formation in cell-free extracts of aerobacter aerogenes 62-1. | the conversion of l-lysine to its corresponding epsilon-n-hydroxy derivative has been achieved for the first time by cell-free extracts of aerobacter aerogenes 62-1. partial fractionation by differential centrifugation (at 12 000 x g) revealed that both supernatant and pellet are essential for maximum enzymatic activity. the omega-n-hydroxylase (ec 1.14.99) was found to function optimally at ph 7-7.5 and exhibited an apparent km of about 75 mum for l-lysine. l(+)-lactate or dl-lactate and pyruva ... | 1977 | 18266 |
| evidence for regulation of aerobacter aerogenes ph 6 acetolactate-forming enzyme by acetate ion. | 1977 | 14639 | |
| coliforms from hides and meat. | coliform tests were performed on 85 hide and 75 meat samples. imvic reactions were determined on isolates from positive confirmed and fecal tests, and strains other than escherichia coli were identified. strains typed as aerobacter aerogenes types i and ii were identified as enterobacter cloacae (51.4%), klebsiella pneumoniae (21.5%), enterobacter aerogenes (15%), and enterobacter liquefaciens, serratia, and unidentified coliforms (12.1%). k. pneumoniae appeared to be responsible for less than 1 ... | 1977 | 319754 |
| studies on the mechanism of the adenosylcobalamin-dependent diol dehydrase reaction by the use of analogs of the coenzyme. | a series of 16 analogs of 5'-deoxy-5'-adenosylcobalamin (adenosylcobalamin) were examined for their effects on the diol dehydrase system of klebsiella pneumoniae (aerobacter aerogenes). four analogs, ara-adenosyl-, aristeromycyl-, 3-isoadenosyl-, and nebularylcobalamin, were able to function as coenzymes in the diol dehydrase reaction, coenzyme activity decreasing in that order. like the native holoenzyme, complexes of the enzyme with these four analogs show a cob(ii)alamin-like absorption peak ... | 1977 | 320203 |
| novel r-plasmid-mediated beta-lactamase from klebsiella aerogenes. | a novel beta-lactamase with a broad spectrum of activity against penicillins and cephalosporins has been detected in a strain of klebsiella aerogenes. its synthesis is mediated by an r-plasmid of molecular weight 64 x 10(6). | 1977 | 329756 |
| isolation of poly-alpha-l-guluronate lyase from klebsiella aerogenes. | the bacterium klebsiella aerogenes type 25 produces an extracellular alginolyase which has been partly purified. the enzyme is specific for the alpha-l-guluronosyl linkages in whole alginate and fractions therefrom. the end products of its action on polyguluronic acid blocks are mainly the unsaturated di- and tri-saccharides, with a smaller proportion of the homologous tetrasaccharide. some general properties of the enzyme are reported. | 1977 | 332364 |
| experience with amikacin and colistin in an outbreak of infection by resistant klebsiella aerogenes. | 1977 | 332682 | |
| explusion of salibi-clamp from the internal carotied artery in the neck by infection. | a left infraclinoid carotid aneurysm (3 x 2-5 x 2 cm) in a 60-year old woman presented with total ophthalmoplegia and trigeminal symptoms. a staged internal carotied occlusion in the neck was performed using a salibi-clamp. a chronic infectious fistula developed (klebsiella aerogenes). when superficial debridement failed, operative removal of the salibi-clamp was planned seven months after its insertion. the clamp was found subcutaneously without any relation to the internal carotid artery and w ... | 1977 | 333303 |
| affinity chromatography od klebsiella arylsulfatase on tyrosyl-hexamethylenediamine-beta-1,3-glucan and immunoadsorbent. | a simple and convenient method for preparation of a highly purified arylsulfatase (ec 3.1.6.1) from klebsiella aerogenes has been developed. specificity of purification was achieved by using affinity chromatography on a tyrosyl-hexamethylenediamino-beta-1,3-glucan or on a solid phase immunoadsorbent. by using affinity chromatography a homogeneous enzyme was obtained with high yield. it is also proposed that the beads of curdlan type polysaccharide consisting of beta-1,3-glucan can be used as a g ... | 1977 | 334267 |
| bacteriology of abscesses of the central nervous system: a multicentre prospective study. | pus from 46 patients with abscesses of the central nervous system (cns) was examined for bacteria; bacteria were found in all patients. streptococci were isolated from 36 patients and most isolates were streptococcus milleri, lancefield group f, ottens and winkler type o iii. staphylococci were isolated from nine patients, organisms of the bacteroides group from 11, proteus spp from seven, klebsiella aerogenes from one, and haemophilus aphrophilus from one. pure cultures predominated over mixed ... | 1977 | 336141 |
| a comparison of alternate metabolic strategies for the utilization of d-arabinose. | mutants of klebsiella aerogenes w70 that metabolize the uncommon pentose d-arabinose were isolated. these mutants were found to be either constitutive or indicible by d-arabinose for the synthesis of enzymes in the l-fucose pathway. such mutants could then utilize l-fucose isomerase to convert the structurally similar d-arabinose molecule to d-ribulose. d-ribulose is an intermediate and the inducer of an existing ribitol pathway and could thus be metabolized. in those d-arabinose-positive mutant ... | 1977 | 338926 |
| hexokinase and glucose-phosphoenolpyruvate phosphotransferase synthesis in klebsiella aerogenes strains growing in continuous culture. | considerable differences in steady-state hexokinase specific activity were found in 16 n.c.i.b. strains of klebsiella aerogenes grown in identical conditions in glucose-limited chemostats. strains of n.c.i.b. 8258 had no detectable activity, but its glucose-phosphoenolpyruvate phosphotransferase specific activity and that of the other strains were closely similar, and it is concluded that this phosphotransferase activity regulates the overall utilization of glucose, in which hexokinase plays no ... | 1977 | 339911 |
| preferential uptake of thymidine by thymineless enterobacteria: its significance in dna labelling. | minimum satisfactory concentrations of thymine and thymidine were determined for the growth of a high thymine-requirng (thy) mutant to escherichia coli strain j5-3. cultures were then grown in the presence of these concentrations of non-radioactive ('cold') pyrimidine together with 5 microci/ml [methyl-3h)thymine, or [methyl-3h)thymidine (specific activities 5 ci/m mole), and the uptake of radioactivity into ice cold trichloroacetic acid insoluble material determined. by far the most efficient l ... | 1977 | 357930 |
| purification and specificity of bacteriolytic enzyme i from hartmannella glebae. | a soil amoeba (hartmannella glebae), when grown in conjunction with enterobacter aerogenes and alcaligenes faecalis, produced two enzymes. enzyme i was purified by gel filtration on sephadex g-100 and chromatography on diethylaminoethyl-cellulose. it is a basic protein. the analysis of the enzymic digest of the cell walls of micrococcus lysodeikticus after reduction and acid hydrolysis showed that the enzyme cleaved the glycosidic bond between acetylmuramic acid and acetylglucosamine of the pept ... | 1977 | 402108 |
| immunochemical comparison of phosphoribosylanthranilate isomerase-indoleglycerol phosphate synthetase among the enterobacteriaceae. | the bifunctional enzyme of the tryptophan operon, phosphoribosylanthranilate isomerase-indoleglycerol phosphate synthetase (prai-ingps;ec 4.1.1.48), was characterized by an immunochemical study of six representative members of the enterobacteriaceae: escherichia coli, salmonella typhimurium, enterobacter aerogenes, serratia marcescens, erwinia carotovora, and proteus vulgaris. prai-ingps was purified from e. coli, and antisera were prepared in rabbits. these antisera were utilized in quantitativ ... | 1977 | 403178 |
| immunochemical evidence for the difference between coenzyme-b12-dependent diol dehydratase and glycerol dehydratase. | klebsiella pneumoniae atcc 25955 (formerly named aerobacter aerogenes pzh 572, warsaw), which is known to produce coenzyme-b12-dependent glycerol dehydratase when grown anaerobically in a glycerol medium, formed coenzyme-b12-dependent diol dehydratase in a 1,2-propanediol-containing medium. both the diol dehydratase and the glycerol dehydratase produced by the organism catalyzed the conversion of glycerol, 1,2-propanediol and 1,2-ethanediol to the corresponding aldehydes and underwent concomitan ... | 1977 | 407082 |
| immunization with r mutants of salmonella minnesota. ii. comparison of the protective effect of immunization with lipid a and the re mutant. | the protective effect of active immunization with salmonella minnesota re bacilli or lipid a was assessed in the granulocytopenic rabbit model. animals were immunized with re bacilli, lipid a, or pseudomonas aeruginosa as a nonspecific immunogen. after colonization with one of three enterobacterial strains (two escherichia coli, one enterobacter aerogenes), the immunized rabbits as well as controls given saline injections were made leukopenic with nitrogen mustard and monitored for fever, bacter ... | 1977 | 407161 |
| noxythiolln-resistant organisms in a district general hospital. | twelve strains of pseudomonas aeruginosa, three strains of klebsiella aerogenes, and two strains of escherichia coli were found to be resistant to noxythiolin. some of the pseudomonads were isolated from patients in the same ward, not all of whom were on noxythiolin treatment. the strains from these patients were indistinguishable from each other on phage typing, which suggested cross-contamination. no gram-positive organism was found to be resistant to noxythiolin. noxythiolin should not be use ... | 1977 | 412549 |
| assymetry of the myo-inositol transport system in klebsiella aerogenes. energy is necessary to create the assymetry of the transport system. | 1. in klebsiella aerogenes the influx of myo-inositol proceeds during the steady state at a rate equal to that of efflux. 2. the kinetic parameters of influx during the steady state are similar to those observed at the initial time of uptake. 3. efflux and influx processes share the same transport system. 4. the ability of other cyclitols to chase the accumulated scyllo-inositol is dependent on their affinity for the transport system. 5. a counter transport can be observed in preloaded cells onl ... | 1977 | 318995 |
| myo-inositol transport in klebsiella aerogenes. scyllo-inositol, a non-metabolizable substrate for the study of the myo-inositol transport system. | 1. scyllo-inositol is transported against a concentration gradient by klebsiella aerogenes, but is not metabolized. 2. the apparent kt of scyllo-inositol is 0.05 x 10(-3) m while that of myo-inositol is 0.23 x 10(-3) m. the v values are respectively 32 and 12 nmol min(-1) mg cells(-1) (dry weight). 3. both cyclitols bind to the same carrier, since typical competitive inhibition kinetics are observed. 4. neither phosphoenolpyruvate phosphotransferase nor a periplasmic binding protein seems to be ... | 1977 | 319004 |
| on the structure of the prosthetic group of citrate (pro-3s)-lyase. | the prosthetic group of citrate (pro-3s)-lyase from klebsiella aerogenes as well as streptococcus diacetilactis was obtained eigher by beta elimination or pronase digestion of the enzyme and purified by deae-cellulose chromatography. the compound was shown to contain 3 mol of po4, 2 mol of ribose, and 1 mol of sulfhydryl/mol of adenine. 5'-amp and dephospho-coa are components of the prosthetic group. the evidence obtained so far support our proposed structure of 3' (or 2') leads to 1''-(5''-phos ... | 1977 | 197081 |
| pathophysiologic responses to klebsiella aerogenes bacteria: 24-hour observations. | 1977 | 364736 | |
| gentamicin-resistant klebsiella aerogenes in a urological ward. | a gentamicin-resistant strain of klebsiella aerogenes was isolated from the urine of 17 patients out of 237 admitted to a male urological ward between jan. 21 and may 9, 1977. the factors most frequently associated with k. aerogenes in the urine were catheterisation and antibiotic therapy. often the epidemic strain (type k16) was found not only in the patients' faeces but also on more remote skin sites such as hands, knees, groins, and the umbilicus. resistance to gentamicin and many other antib ... | 1977 | 70654 |
| fatty acid compositions of lipid fractions from vegetative cells and mature sorocarps of the cellular slime mold dictyostelium discoideum. | a wild-type strain of dictyostelium discoideum was grown upon aerobacter aerogenes. fatty acid compositions of lipid fractions and of total lipids obtained from vegetative amoebae and mature sorocarps were compared. fatty acids isolated from vegetative cells were found to include large quantities of 17- and 19-carbon cyclopropane fatty acids while straight-chain, saturated fatty acids represented only 10% (w/w) of total fatty acids. these cyclopropane fatty acids appear to be derived from ingest ... | 1977 | 559228 |
| laboratory studies on mecillinam: activity alone and combined with cephradine. | mecillinam (mc) was found to be inhibitory at low concentrations to a wide range of gram-negative bacteria; the sensitivity of such strains could be predicted by using a 10 microgram disk. resistance to mc did not appear to be associated with beta-lactamase-mediated destruction of the antibiotic. ampicillin-resistant strains tended to be less sensitive to mc. mc and cephradine acted synergistically against proteus mirabilis, pr. vulgaris, prov. stuartii and serratia marcescens, and the combinati ... | 1977 | 587708 |
| microbial conversion of dl-2-amino-delta2-thiazoline-4-carboxylic acid to l-cysteine and l-cystine: screening of microorganisms and identification of products. | microorganisms able to form l-cysteine from dl-2-amino-delta2-thiazoline-4-carboxylic acid (dl-atc), a chemical intermediate in the synthesis of dl-cysteine, were isolated from soil samples and classified as pseudomonas sp., pseudomonas cohaerens, p. desmolytica, and p. ovalis. thirteen l-cysteine-producing bacteria were also found in among 463 stock cultures representing 37 genera. these were achromobacter delmarvae. alcaligenes denitrificans, bacillus brevis, brevibacterium flavum, enterobacte ... | 1977 | 596877 |
| transport equations for a microbial predator-prey community. | a transport equation is used which describes the temporal behavior of interacting populations in changing environments. the formulation takes into account the internal state variables of the individuals. the general theory is applied to the transient analysis of a microbial predator-prey system using an approximate model for the specific cell growth rate and multigroup formulism to approximate the mass distribution within the population. experimental results in atetrahymena pyriformis- aerobacte ... | 1977 | 24233466 |
| tryptophan operon regulation in interspecific hybrids of enteric bacteria. | we examined tryptophan regulation in merodiploid hybrids in which a plasmid carrying the trp operon of escherichia was introduced into trp mutants of other enteric genera, or in which a plasmid carrying the trpr+ (repressor) gene of e. coli was transfered into fully constitutive trpr mutants of other genera. in these hybrids the trp operon of one species is controlled by the repressor of a different species. similar investigations were possible in transduction hybrids in which either the trp ope ... | 1976 | 770450 |
| derivation and properties of f-prime factors in escherichia coli carrying nitrogen fixation genes from klebsiella pneumoniae. | a his+ nif+ escherichia coli k12, hfr strain (unf43) was constructed by an intergeneric mating between a klebsiella pneumoniae donor strain (hf3) and a his-hfr e. coli strain (sbi824) which transfers his as an early marker. an f-prime nif plasmid, fn39, carrying genes which correspond to the e. coli chromosomal region, metg gnd his shia, but excluding purf and arod, was isolated from unf43. translocation of carbenicillin resistance genes from a p-type r-factor, r68, to fn39 increased the stabili ... | 1976 | 772161 |
| bactericidal activity of bovine neonatal serums for selected coliform bacteria in relation to total protein and immunoglobulin g1 and immunoglobulin m concentrations. | bactericidal activity for a serum-sensitive aerobacter aerogenes strain was associated with antibodies present in immunoglobulin g1 (igg1) and immunoglobulin m (igm), with the greatest activity on a weight basis in igm. activity in immunoglobulin g2 was absent. a total of 118 serums were prepared from blood collected from neonatal calves on farms experiencing unusually high mortality from diarrhea. the serums were allotted to 4 groups on the basis of total serum protein concentrations as follows ... | 1976 | 773223 |
| subunit and chemical composition of citrate lyase from klebsiella pneumoniae. | citrate lyase from klebsiella pneumoniae (previously called klebsiella aerogenes) is shown by amino acid sequencing techniques to contain equimolar amounts of three nonidentical subunits. together with the molecular weight of the enzyme, this finding is interpreted to indicate that there are 6 mol of each of these subunits per mol of native enzyme. a partial amino acid sequence of each of the three subunits is presented. the pure enzyme with a specific activity of 73 units/mg of dry protein has ... | 1976 | 773936 |
| the effect of nitrogen limitation on catabolite repression of amidase, histidase and urocanase in pseudomonas aeruginosa. | in pseudomonas aeruginosa, the synthesis of histidase, urocanase and amidase is severly repressed when succinate is added to a culture growing in pyruvate + ammonium salts medium. when growth is nitrogen-limited, catabolite repression by succinate of histidase and urocanase synthesis does not occur but succinate repression of amidase synthesis persists. amidase synthesis is not regulated in the same way as histidase synthesis by the availability of other nitrogen compounds for growth. growth of ... | 1976 | 6623 |
| regulation of enzyme formation in klebsiella aerogenes by episomal glutamine synthetase of escherichia coli. | we studied the physiology of cells of klebsiella aerogenes containing the structural gene for glutamine synthetase (glna) of escherichia coli on an episome. the e. coli glutamine synthetase functioned in cells of k. aerogenes in a manner similar to that of the k. aerogenes enzyme: it allowed the level of histidase to increase and that of glutamate dehydrogenase to decrease during nitrogen-limited growth. the phenotype of mutations in the glna site was restored to normal by the introduction of th ... | 1976 | 6431 |
| glutamate dehydrogenase: genetic mapping and isolation of regulatory mutants of klebsiella aerogenes. | the gene for glutamate dehydrogenase (gdhd) has been mapped in klebsiella aerogenes by p1 transduction. it is linked to pyrf and trp with the order pyrf-trp-gdh. complementation analysis using f' episomes from escherichia coli suggests an analogous location in e. coli. two mutants able to produce glutamate dehydrogenase in the presence of high levels of glutamine synthetase have been isolated. one, tightly linked to gdhd, shows normal repression control by glutamine synthetase but produces four ... | 1976 | 6429 |
| the existence of three types of acetohydroxy acid synthetase in an isoleucine-requiring mutant of aerobacter aerogenes. | the synthesis of the three types of acetolactate synthase (ec 4.1.3.18) which are responsible for the biosynthesis os isoleucine and valine, was observed in aerobacter aerogenes i-12, an isoleucine-requiring mutant, when grown on the four kinds of media. when the cells were grown on isoleucine-rich medium, acetolactate synthase sensitive to feedback inhibition and having an optimum ph at 8.0 was formed. by increasing the amount of potassium phosphate in the medium, the catabolite repression of t ... | 1976 | 5136 |
| l-asparaginase of klebsiella aerogenes. activation of its synthesis by glutamine synthetase. | an l-asparaginase has been purified some 250-fold from extracts of klebsiella aerogenes to near homogeneity. the enzyme has a molecular weight of 141,000 as measured by gel filtration and appears to consist of four subunits of molecular weight 37,000. the enzyme has high affinity for l-asparagine, with a km below 10(-5) m, and hydrolyzes glutamine at a 20-fold lower rate, with a km of 10(-3) m. interestingly, the enzyme exhibits marked gamma-glutamyltransferase activity but comparatively little ... | 1976 | 4459 |
| effect of environmental factors on inactivation of b12-dependent glycerol dehydratase from aerobacter aerogenes. | effect of temperature, ph and univalent cation on kinetics of self-activation of b12-dependent glycerol dehydratase (gd) from aerobacter aerogenes with co alpha-alpha-(5,6-dimethylbenzimidazolyl-co beta-adenosylcobamide (adocbl) was investigated. the activation energy of the process of gd inactivation is found to be 3.9 kkal/m, the effect of ph on gd inactivation being insignificant. monovalent cation is not required for the formation of gd-adocbl complex, but it protects the complex from selfin ... | 1976 | 11842 |
| nutritional requirement for 4-aminobenzoate caused by mutation of dihydropteroate synthetase. | aerobacter aerogenes mutant 62-1 ac requires high concentrations of 4-aminobenzoate for growth. the mutant accumulates n-glucosyl-4-aminobenzoate and has an intact 4-aminobenzoate synthetase (bacher, gilch, rappold, and lingens, z. naturforsch. 28 c, 614-617 [1973]). on the other hand the ability of the mutant to synthesize dihydropteroate is markedly reduced. the dihydropteroate synthetase level of mutant 62-1 ac is 1% as compared to the parent strain. spontaneous revertants of mutant 62-1 ac s ... | 1976 | 134570 |
| spore germination promoter of dictyostelium discoideum excreted by aerobacter aerogenes. | the nutrient medium in which aerobacter aerogenes was grown, contains a spore germination promoter (sgp) for the cellular slime mould dictyostelium discoideum. sgp can cuase synchronous spore germination in a short time, and triggers the germination process in just a few minutes. germination-promoting capacity of sgp decreases as it comes in contact with increasing number of spores. when spores activated by sgp are stored at 4 degrees c, they gradually return to the dormant state. sgp is compara ... | 1976 | 9416 |
| studies on inosine monophosphate dehydrogenase. steady state kinetics. | the reaction catalyzed by imp dehydrogenase (imp: nad+ oxidoreductase ec 1.2.1.14) from aerobacter aerogenes has been investigated kinetically at ph 8.1 as a three reactant system by means of steady-state velocity studies in the absence of products, as well as by inhibition studies using products and substrate analogues. the mechanism appears to be a partially random one in which imp and k+ can bind randomly to the free enzyme while nad does not react unless k+ or both k+ and imp are present on ... | 1976 | 178371 |
| natural and altered induction of the l-fucose catabolic enzymes in klebsiella aerogenes. | mutants of klebsiella aerogenes w70 were isolated that had gained the ability to utilize the uncommon pentose d-arabinose as their sole source of carbon and energy. in contrast to the d-arabinose-negative, parent strain, these mutants were found to be either constitutive for certain enzymes of the l-fucose catabolic pathway or inducible for such enzymes when incubated in the presence of d-arabinose. the mutants used l-fucose isomerase to convert d-arabinose to d-ribulose, which is an intermediat ... | 1976 | 179982 |
| energetic aspects of anaerobic growth of aerobacter aerogenes in complex medium. | molar growth yields for anaerobic growth of aerobacter aerogenes in complex medium were much higher than for growth in minimal medium. in batch cultures the molar growth yield for glucose varied from 44 to 50 and yatp from 17.1 to 18.8. for glucose-limited chemostat cultures a value of 17.5 g/mole was found for y max atp and a value of 2.3 mmoles atp/g dry weight h for the maintenance coeficient. growth-dependent ph changes were used to control the addition of fresh medium, containing excess of ... | 1976 | 13757 |
| a microcalorimetric study of the growth of klebsiella aerogenes in simple salts/glucose media. | heat output-time records or 'thermograms' produced during the aerobic growth of klebsiella aerogenes in simple salts/glucose media with growth limiting glucose concentrations of 2.0, 1.0 and 0.5 g dm-3 were obtained using a flow-microcalorimeter fitted with an aerobic cell. these traces are interpreted in terms of the recorded oxygen tension, ph, glucose concentration and bacterial population of the culture. heat output is greatest during the phase of exponential growth, indicating that here the ... | 1976 | 17049 |
| transduction of chromosomal genes between enteric bacteria by bacteriophage p1. | we have used p1 transduction to create intergeneric hybrid strains of enteric bacteria by moving the gena and hut genes between klebsiella aerogenes, escherichia coli and salmonella typhimurium. the use of e. coli as the recipient in such transductions permits the construction of episomes and specialized transducing phage containing non-e. coli material. the effect of host restriction modification and deoxyribonucleic acid homology on the frequency of intergeneric transduction of these loci has ... | 1976 | 3494 |