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chronic hepatitis associated with leptospiral infection in vaccinated beagles.sixteen juvenile beagle dogs originating from a single breeding colony and regularly vaccinated against leptospira interrogans (serogroups canicola and icterohaemorrhagiae) developed a clinical syndrome characterized by retarded growth, weight loss and often ascites. over a 10-month period, post-mortem examinations were performed on all affected dogs. gross lesions were confined to the liver which was often firm, tan-coloured and mottled. microscopically, hepatic lesions ranged from those of sev ...19979502268
pcr amplification, molecular cloning, dna sequence analysis and immuno/protection in balb/c mice of the 33 kda endoflagellar protein of l. interrorgans serovar lai.a pair of oligonucleotide primers were designed to amplify the endoflagella gene of l. interrogans serovar lai. an approximately 840 bp fragment was generated with pcr and inserted into plasmid puc8, after the fragment and puc8 were digested respectively with bamhi and psti. a recombinant plasmid (designated as plf1) was obtained. sds-page analysis indicated that a 33 kda was expressed in e. coli jm103 harboring plf1 and the expression level of the protein was 11% of the total bacterial soluble ...199711243093
rapid identification of pathogenic leptospira species (leptospira interrogans, l. borgpetersenii, and l. kirschneri) with species-specific dna probes produced by arbitrarily primed pcr.arbitrarily primed pcr (ap-pcr) assays can be used to discriminate between species of leptospira. comparative analysis of the fingerprints obtained from representative sets of serovar reference strains of leptospira interrogans sensu stricto, l. borgpetersenii, and l. kirschneri and the reference strains of the other leptospira spp. revealed species-specific dna fragments. these species-specific sequences were reamplified in order to produce digoxigenin-11-dutp-labeled genomic dna probes that co ...19978968917
effect of leptospira interrogans serovar hardjo infection on progesterone concentrations in heifers. 19979004477
invasion of vero cells and induction of apoptosis in macrophages by pathogenic leptospira interrogans are correlated with virulence.interactions of virulent leptospira interrogans serovar icterohaemorrhagiae strain verdun with vero cells (african green monkey kidney fibroblasts) and a monocyte-macrophage-like cell line (j774a.1) were assayed by a double-fluorescence immunolabelling method. infectivity profiles were investigated according to (i) the duration of contact between leptospires and eukaryotic cells and (ii) the number of in vitro passages after primary isolation from lethally infected guinea pigs. comparative exper ...19979009336
superoxide dismutase activity and lipid peroxidation in the liver of guinea pig infected with leptospira interrogans.superoxide dismutase (sod) activity and the degree of lipid peroxidation were studied over a two week period in guinea pigs infected with leptospira interrogans derived from wild mice. the total sod activity in infected host liver increased by four-fold two days after infection; this was followed by a 20% decrease resulting in levels comparable to normal, uninfected liver. during the period of decreasing sod activity after day two, the levels of tba-reactive material (tbars) are increased by thr ...19979018467
identification and characterization of the dtdp-rhamnose biosynthesis and transfer genes of the lipopolysaccharide-related rfb locus in leptospira interrogans serovar copenhageni.immunity to leptospirosis is principally humorally mediated and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. the only protective antigen identified to date is the leptospiral lipopolysaccharide (lps), which biochemically resembles typical gram-negative lps but has greatly reduced endotoxic activity. little is known about the structure of leptospiral lps. a 2.1-kb ecori fragment from the chromosome of serovar copenhageni was cloned in puc18 in escherichia ...19979023210
clinical and clinicopathologic abnormalities in greyhounds with cutaneous and renal glomerular vasculopathy: 18 cases (1992-1994)to determine clinical signs and clinicopathologic abnormalities in greyhounds with cutaneous and renal glomerular vasculopathy and to determine whether there were any differences between dogs with and without renal azotemia.19979074681
protein engineering reveals ancient adaptive replacements in isocitrate dehydrogenase.evolutionary analysis indicates that eubacterial nadp-dependent isocitrate dehydrogenases (ec 1.1.1.42) first evolved from an nad-dependent precursor about 3.5 billion years ago. selection in favor of utilizing nadp was probably a result of niche expansion during growth on acetate, where isocitrate dehydrogenase provides 90% of the nadph necessary for biosynthesis. amino acids responsible for differing coenzyme specificities were identified from x-ray crystallographic structures of escherichia c ...19979096353
purification and characterization of a na+, k+ atpase inhibitor found in an endotoxin of leptospira interrogans.we showed previously that the glycolipoprotein fraction prepared from leptospira interrogans inhibited the na+,k+ atpase enzyme purified from brain or kidney and in isolated nephron segments (m. younes-ibrahim, p. burth, m. v. castro faria, b. buffin-meyer, s. marsy, c. barlet-bas, l. cheval, and a. doucet, c. r. acad. sci. paris ser. iii 318:619-625, 1995). in the present communication, we have demonstrated that unsaturated fatty acids such as oleic and palmitoleic acids, which are adsorbed to ...19979119504
detection of an antigenic protein of leptospira interrogans which shares epitopes with the equine cornea and lens.a protein epitope which is involved in an antigenic relationship between equine ocular tissues and leptospira interrogans was detected in homogenates of the bacterium. the antigenic determinant was harboured on a peptide structure which was shown to be sensitive to the action of denaturing and reducing agents by means of western blotting. the outer surface of the leptospires appeared to be free of this epitope as was proved by dot-blot and electron microscopic studies.19979125359
outcome of leptospirosis in children.we conducted a retrospective analysis of 43 consecutive children (35 boys and 8 girls), 4-14 years of age and living in an urban area, who were hospitalized at the instituto de infectologia emilio ribas (sao paulo, brazil) from january 1989 to december 1995 with an acute illness subsequently diagnosed as leptospirosis. epidemiologic data indicated contact with contaminated water in most cases (88%). the patient sera reacted most strongly with leptospira interrogans serovars copenhageni (45%) and ...19979129533
leptospirosis in brushtail possums: is leptospira interrogans serovar balcanica environmentally transmitted?in new zealand, the biological control of introduced brushtail possums (trichosurus vulpecula) may be the only affordable option for achieving a significant long term reduction in pest numbers on a national scale. leptospira interrogans serovar balcanica is among the potential biocontrol agents and vectors currently being investigated for this purpose. as the transmission pathways of l. interrogans serovar balcanica between possums are poorly understood, the objective of the study was to determi ...19979131555
serologic survey for infectious pathogens in free-ranging american bison.from november 1991 through march 1992, we evaluated 101 free-ranging american bison (bison bison) from yellowstone national park, wyoming (usa) for exposure to infectious organisms that commonly infect cattle. no titers were detected for bluetongue virus, bovine leukemia virus, or campylobacter fetus in these 101 bison. detectable antibodies occurred against anaplasma marginale (eight of 76, 11%), bovine respiratory syncytial virus (31 of 101, 31%), bovine viral diarrhea (31 of 101, 31%), bovine ...19979131564
rapid distinction between leptospira interrogans and leptospira biflexa by pcr amplification of 23s ribosomal dna.bacterial specific primers were used to amplify 23s rrna genes from a representative strain from each of the 23 serogroups of the pathogenic leptospira interrogans and 8 strains from 6 serogroups of the non-pathogenic leptospira biflexa. only regions of extreme variability, which had been identified on the basis of homology-based search of all the 23s rrna sequences available in genbank database, were sequenced from the amplified products. pcr primers that had the potential to distinguish l. int ...19979163900
leptospirosis in raccoons in quebec: 2 case reports and seroprevalence in a recreational area.raccoons may represent a source of leptospires for humans and domestic animals. we describe a case of severe interstitial nephritis associated with the serovar bratislava of leptospira interrogans (1st report in wildlife), and the seroprevalence to 4 leptospire serovars in a recreational area in quebec.19979220134
evaluation of a commercial enzyme-linked immunosorbent assay for detection of immunoglobulin m antibody in diagnosis of human leptospiral infection.the panbio leptospira immunoglobulin m (igm) enzyme-linked immunosorbent assay (elisa) is a commercially available screening test for the diagnosis of acute leptospiral infection. the ability of the test to diagnose early or recent leptospira interrogans infection was assessed by testing sera with known microagglutination test (mat) titers to serovars pomona, hardjo, copenhageni, and australis. the igm elisa detected all 41 cases of early or recent leptospiral infection (sensitivity, 100%), with ...19979230359
immunodiagnosis of human leptospirosis by dot-elisa for the detection of igm, igg, and iga antibodies.a dot-elisa was evaluated using antigen obtained from leptospira interrogans cultures of the serovars brasiliensis, canicola, cynopteri, hebdomadis, and icterohaemorrhagiae for the detection of human igm, igg, and iga. single serum samples from 63 patients with the icterohemorrhagic form of leptospirosis in the acute phase, collected 3-14 days (mean = 7 days) after the onset of symptoms were tested. ten patients were examined during convalescence and followed up for a period of 4-12 months. for ...19979230798
leptospira interrogans serovar icterohaemorrhagiae seropositivity and the reproductive performance of sows.the reproductive performance of 28 sows seropositive to leptospira interrogans serovar icterohaemorrhagiae was compared with that of 87 leptospira sp. seronegative dams belonging to the same herd. sows were sampled during 1988 to 1993. during this period the herd was not submitted to any kind of intervention (antibiotic therapy, immunoprophylaxis or rodent control). relative risks (rr) of return to heat, mummified fetuses, stillbirth, and weak newborn piglets for infected sows were assessed and ...19979234428
na,k-atpase: a molecular target for leptospira interrogans endotoxin.on the basis of our report that a glycolipoprotein fraction (glp) extracted from leptospira interrogans contains a potent inhibitor of renal na,k-atpase, we proposed that glp-induced inhibition of na,k-atpase might be the primary cellular defect in the physiopathology of leptospirosis. the present study was designed to test this hypothesis by determining whether or not 1). glp inhibits all the isoforms of na,k-atpase which are expressed in the tissues affected by leptospirosis, 2) na,k-atpase fr ...19979239307
first epidemiological data on pathogenic leptospires isolated on the azorean islands.insectivores (erinaceus europaeus) and rodents (rattus rattus, r. norvegicus and mus musculus) from different islands of the azores archipelago were found to carry three distinct leptospira interrogans s.l. serovars (copenhageni, icterohaemorrhagiae and ballum) which have never been previously investigated there. the house mouse and the black rat were the major leptospira reservoirs showing isolation rates ranging from 0% for both species (in graciosa) to 88% and 33%, respectively (in sãao migue ...19979258550
[leptospira interrogans in a canine population of greater buenos aires: variables associated with seropositivity].we determined the seroprevalence of leptospirosis in a suburban canine population for the purpose of analyzing the association between different individual and environmental variables and seropositivity for leptospirosis. the study, which was cross-sectional, was performed in july 1992 in a neighborhood of greater buenos aires with approximately 9,500 inhabitants and a canine population of around 2,000 animals. we studied a random sample of 223 dogs and obtained a blood sample from each. each an ...19979312417
differentiation of leptospira interrogans isolates by is1500 hybridization and pcr assays.genetic variability among leptospira interrogans (sensu stricto) serovars was assessed by southern blot hybridization and pcr analyses. the experiments used probes directed to sequences in a recently described insertion element, is1500. hybridization analysis showed that is1500 was present on polymorphic fragments and that differences in these patterns could be used to identify serovars. hybridization analysis was also useful in discriminating between serovar pomona type kennewicki isolates, mak ...19979316917
identification of a 35-kilodalton serovar-cross-reactive flagellar protein, flab, from leptospira interrogans by n-terminal sequencing, gene cloning, and sequence analysis.during the screening of antibodies to pathogenic leptospires, a murine monoclonal antibody (designated m138) was found to react with various serovars. an antigen of approximately 35 kda from leptospira interrogans serovar pomona, which reacted strongly with m138, was characterized by n-terminal amino acid sequencing and identified as a flagellin, a class b polypeptide subunit (flab) of the periplasmic flagella. the gene encoding the flab protein, flab, was amplified from the genomic dna of sever ...19979317049
risk factors associated with the likelihood of leptospiral seropositivity in horses in the state of new york.to determine and quantify risk factors associated with exposure of horses to the following serovars of leptospira interrogans: pomona, autumnalis, and bratislava.19979328661
development and initial evaluation of an indirect enzyme-linked immunosorbent assay for the detection of leptospira interrogans serovar hardjo antibodies in bovine sera.outer sheath antigen from leptospira interrogans serovar hardjo type hardjoprajitno and acetic acid extracted antigens from serovar hardjo types hardjoprajitno and hardjobovis were evaluated in an immunoassay for ability to detect hyperimmune rabbit serum to serovar hardjo. the degree of cross-reactivity with hyperimmune rabbit sera to l. interrogans serovars pomona, copenhageni, grippotyphosa, canicola and sejroe, and leptospira biflexa serovar patoc was also measured for each antigen. all of t ...19979342449
development of a monoclonal antibody-based competitive enzyme-linked immunosorbent assay for the detection of leptospira borgpetersenii serovar hardjo type hardjobovis antibodies in bovine sera.a murine monoclonal antibody (designated m553) that binds to an epitope on whole cell antigens prepared from leptospira borgpetersenii serovar hardjo type hardjobovis and leptospira interrogans serovar hardjo type hardjoprajitno, was produced and incorporated into a competitive enzyme-linked immunosorbent assay for the detection of bovine antibodies to serovar hardjo. the epitope recognized by m553 was susceptible to periodate oxidation. the m553 antibody was characterized by western blot with h ...19979342450
simplified analysis of pathogenic leptospiral serovars by random amplified polymorphic dna fingerprinting.a rapid, simplified procedure combining random amplified polymorphic dna (rapd) fingerprinting of boiled cultures with high resolution agarose gel electrophoresis was used to compare strains from 46 pathogenic leptospiral serovars. the serovars were placed in eight groups on the basis of rapd profile similarities. groups 1-7 corresponded with the genome species leptospira interrogans, l. borgpetersenii, l. santarosai, l. noguchii, l. weilii, l. kirschneri and l. meyeri. the eighth group did not ...19979368533
effect of leptospira interrogans endotoxin on renal tubular na,k-atpase and h,k-atpase activities. 19979405892
[development of polymerase chain reaction-based test systems for detecting leptospira in polytypical leptospirosis foci].two highly sensitive test systems g and b, based on the polymerase chain reaction, were developed for indication of pathogenic leptospira interrogans, including the serovariants appearing during outbreaks in polytypical foci of leptospirosis in the tropical zone of china. these test systems can be used for rapid diagnosis of leptospirosis in humans in foci with different etiological structure.19979411214
preliminary survey of leptospirosis and lyme disease amongst febrile patients attending community hospital ambulatory care in maputo, mozambique.to evaluate the importance of the spirochetes leptospira interrogans s.l. and borrelia burgdorferi s.l., as causes of human diseases (leptospirosis and lyme borreliosis), in order to guide the development of laboratory services and patient management and to identify the appropriateness of future epidemiological studies.19979431763
[the pcr amplification, cloning, sequencing, expression in e. coli of gene encoding endoflagella subunit protein (fla b) from leptospira interrogans serovar lai].a pair of oligonucleotide primers were designed by ourselves to amplify the endoflagella gene of l. interrogans serovar lai. a fragment about 840 bp was generated with pcr and inserted into plasmid puc8 after the fragment and puc8 were digested respectively with bam hi and pst i. a recombinant plasmid (designated as plf1) was obtained. sds-page analysis indicated that a 33 kd was expressed in e. coli jm103 harboring plf1 and the expression level of the protein was 11% of total bacterial soluble ...19969208612
[16s rrna gene pcr-sscp analysis of the reference strains from 15 serovars (14 serogroups) of pathogenic leptospires in china].the dnas of reference strains from 15 serovars (14 serogroups) of pathogenic leptospires in china were amplified with 16s rrna gene primers, and then single-strand conformation polymorphism (sscp) of the products were analyzed in 12.5% nondenaturing mini polyacrylamide gel (containing 5% glycerol) combining with silver-staining. all products showed two bands on electrophoresis at different parameters of voltage or current and concentration of gel. it proved that serovar lai, serovar canicola, se ...19969208613
leptospira interrogans and leptospira peptidoglycans induce the release of tumor necrosis factor alpha from human monocytes.elevated plasma concentrations of the cytokine tumor necrosis factor alpha (tnf alpha) have been observed in patients affected by leptospirosis. in this study we found that a preparation of peptidoglycan of leptospira interrogans, serovar copenhageni, was able to induce the release of tnf alpha from peripheral blood mononuclear cells. tnf alpha induction occurred in a dose dependent manner and was not affected by the endotoxin inhibitor polymixin b. this is the first report on induction of tnf a ...19969026448
preliminary evaluation of antimicrobial agents for treatment of leptospira interrogans serovar pomona infection in hamsters and swine.to evaluate antimicrobial agents for treatment of models of acute and persistent leptospirosis caused by leptospira interrogans serovar pomona.19968720239
leptospira interrogans serovar hardjo in the kidneys and genital tracts of naturally infected sheep.a bacteriological study was carried out to identify possible renal and/or genital carriers of leptospira interrogans serovar hardjo. l. hardjo was found at slaughter in the kidneys of three seropositive ewes, but not in uterus or salpinges of these animals.19968722315
effect of vaccination against leptospira interrogans serovar hardjo on milk production and fertility in dairy cattle. 19968730678
leptospira interrogans exposure in free-ranging elk in washington.exposure to one or more serovars of leptospira interrogans was observed in five of six sampled elk (cervus elaphus roosevelti) killed in november 1993, from an isolated herd in southwest washington, usa (46 degrees 45'n, 123 degrees 6'w). in april 1994, exposure to l. interrogans serovars was documented in nine of 11 captured cow elk from the same herd. leptospires were not isolated from any of the exposed elk, and 10 of the 11 cows were pregnant. the high seroprevalence is evidence that exposur ...19968627923
isolation of leptospira interrogans serovar grippotyphosa from a heifer in new south wales. 19968660211
presence of antigen and antibodies in serum and genital discharges of heifers after experimental intrauterine inoculation with leptospira interrogans serovar hardjo.the excretion of leptospira interrogans serovar hardjo in cervico-vaginal mucus (cvm) or urine and the local and systemic immune responses to the organism were monitored in eight susceptible heifers after intrauterine inoculation while six similar heifers served as controls. all the heifers were inseminated at the subsequent oestrous periods. the overall percentage pregnancy rate (the number of pregnancies divided by the total number of inseminations) was lower in the infected heifers than in th ...19968685538
presence of antigen and antibodies in serum and genital discharges of cows from dairy herds naturally infected with leptospira interrogans serovar hardjo.samples of cervico-vaginal mucus from 163 bulling cows (group 1) and post calving discharges from 59 newly calved cows (group 2) in five dairy herds naturally infected with leptospira interrogans serovar hardjo were examined for the presence of antigen and igg and iga antibodies by using two elisa systems which were protein or carbohydrate based. corresponding serum samples were examined for systemic immune responses by using a microscopic agglutination test (mat) and igg-elisa tests. antigen wa ...19968685539
reproductive performance of dairy herds infected with leptospira interrogans serovar hardjo relative to the year of diagnosis.to assess the impact of leptospira interrogans serovar hardjo infection on the reproductive performance of nine dairy herds with evidence of infection, forty years' fertility data were analysed relative to the year of first diagnosis. fifty per cent of various fertility variables had their lowest values only in the year of diagnosis. culling rates were highest during the year of diagnosis in five of the herds, and were above 22 per cent in five of nine (55-6 per cent) of the diagnosis years cons ...19968711883
rapid distinction between leptonema and leptospira by pcr amplification of 16s-23s ribosomal dna spacer.the pcr amplification of the genomic dna of leptonema illini strain 3055 using primers directed against conserved regions of the rrna operon provided evidence that the 16s and 23s rrna genes were linked via an intergenic spacer region. the sequencing of the intergenic spacer region indicated that it was 435 nucleotides in length and sequence similarity searches revealed that it bore no homology to any known sequences including trna available in databases. further investigations using southern bl ...19968759793
intestinal spirochetosis: first cases reported in brazil and the use of immunohistochemistry as an aid in histopathological diagnosis.colonization of the colon and rectum by intestinal spirochetes is detected for the first time in brazil in 4 of 282 (1.41%) patients who had undergone sigmoidoscopy and/or colonoscopy with a histopathological diagnosis of chronic non specific-colitis. this frequency is probably underestimated, since surgically obtained specimens were not considered in the present study. histopathological diagnosis was performed using routine stains like hematoxylin-eosin which showed the typical, of 3-microns th ...19968762639
[the molecular genetic and biological aspects of the ecology of pathogenic leptospira (leptospira interrogans)].the results of recent investigations on the application of some genotyping methods (genomic fingerprinting, analysis of restriction length fragment polymorphism of dna, etc.), made in order to study the population structure of pathogenic leptospires and to evaluate their intraspecies heterogeneity with regard to their main ecological features, are reviewed. new data on the use of prc-based amplification test systems for the study of specific features of host persistence of leptospires are presen ...19968771722
experimental immunization of hamsters with an edta extract of leptospira interrogans, serovar icterohaemorrhagiae.the edta extract was prepared from the leptospira interrogans serovar icterohaemorrhagiae. when inoculated subcutaneously in hamsters it conferred protection from challenge with virulent leptospires with a low dose (10 micrograms/ml) and low agglutinating antibody (40). the protection of animals obtained by the edta extract opens up the perspective of its use as a component of a vaccine for the control of leptospirosis.19968783904
correlation between dna restriction fragment length polymorphisms in leptospira interrogans serovar pomona type kennewicki and host animal source.isolates (n = 147) of leptospira interrogans serovar pomona type kennewicki from cattle, swine, horses, and wildlife were analyzed by dna restriction endonuclease analysis. restriction fragment length polymorphisms were identified in dna digested with hpaii, and the restriction fragment length polymorphisms were correlated with the host animal source of the isolates. these results will be useful in understanding the epidemiology of serovar pomona infections in livestock.19968789028
acute renal disease due to leptospira interrogans in a weanling. 19968818600
leptospira interrogans serovar grippotyphosa infection in dogs.leptospirosis attributed to infection with serovar grippotyphosa was diagnosed in 11 dogs. in naturally and experimentally infected dogs, a stereotypic serologic response to infection with leptospira serovar grippotyphosa was detected. although the highest serum antibody titers developed against serovar grippotyphosa, most dogs also had lower titers against serovars bratislava and pomona. acute renal failure was evident in 10 dogs. one dog died prior to initiation of treatment; the remaining 10 ...19968837647
leptospira interrogans in the genital tract of sheep. research on ewes and rams experimentally infected with serovar hardjo (hardjobovis).to verify if leptospira hardjo can colonize the male and female genital organs of sheep, 9 animals (6 non pregnant ewes and 3 mature rams) were infected with a strain of l. hardjobovis recently recovered from the kidneys of a seropositive ewe. postinfection controls (bacteriologic, serologic, immunohistochemistry and electron microscopy) failed to disclose the presence of leptospires in the uterus and oviducts, testicles, epididymis, prostate and bulbourethral glands of animals used for the expe ...19968841039
reduced conception rates in dairy cattle associated with serological evidence of leptospira interrogans serovar hardjo infection.fertility data from 673 cows in five dairy herds with a moderate to high seroprevalence of microscopic agglutination titres (mat) of > or = 1:10 against leptospira interrogans serovar hardjo were collated to assess the relationship between pregnancy rates and antibody titres to serovar hardjo. a significant proportion of failures of conception (10 to 13 per cent, p < 0.001) were associated with mats of 1:10 to 1:100; the overall pregnancy rate of the seronegative cows was 28.5 per cent higher (p ...19968856888
protective activity of rabbit polyclonal anti-idiotype antibody against leptospira interrogans infection in hamsters.we prepared an anti-idiotype (id) antibody against leptospirosis. serum from rabbit immunized with monoclonal antibody (mab) lw2, which reacted to the main protective antigen prepared from leptospira interrogans serovar lai, inhibited agglutination of the organism by mab lw2. the immune rabbit serum was applied to a column coupled with normal mouse igg as a ligand (first column), and the unbound fraction eluted was applied to a column coupled with mab lw2 as a ligand (second column). the bound f ...19968860969
the development of a ligase mediated pcr with potential for the differentiation of serovars within leptospira interrogans.a ligase mediated polymerase chain reaction (lmpcr) was developed to amplify between the repetitive element, is1533, of leptospira and adjacent chromosomally located bg/ii restriction endonuclease enzyme sites. to do this, complimentary oligonucleotide linkers designed to anneal together with an overhanging bg/ii end were ligated to bg/ii digested dna from 35 leptospiral reference strains and field isolates. this ligated dna was used as template for pcr with oligonucleotide primers specific for ...19968870196
production and characterization of monoclonal antibodies to the edta extract of leptospira interrogans, serovar icterohaemorrhagiae.monoclonal antibodies (mabs) were produced against an ethylenediaminetetraacetate (edta) extract of leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as igm and igg (igg1 and igg2b). the edta extract was detected as several bands by silver staining in sds-page. in the western blot the bands around 20 kda reacted with a monoclonal antibody, 47b4d6, and was oxidized by periodate and was not digested by pronase, suggesting that the determinant is of carbohy ...19968885673
effect of leptospira interrogans serovar hardjo infection on milk yield in endemically infected dairy herds. 19968893491
restriction fragment length polymorphisms in australian and new zealand isolates of leptospira interrogans serovar pomona. 19968894026
detection of leptospiral plasmid and comparison of plasmid profiles between virulent and avirulent leptospires.detection of plasmid from leptospires and a comparison of the plasmid profiles between virulent and avirulent strains were performed to investigate whether leptospires contained plasmid(s) associated with virulence. virulent strains of leptospira interrogans serovars copenhageni, lai, canicola and pomona, which were virulent for the guinea pig and/or hamster and which showed chemotaxis toward hemoglobin, contained approximately 370 kilobases (kb) plasmid. avirulent strains of l. interrogans also ...19968898293
sporadic urban leptospirosis.surprisingly, many inner-city residents have antibodies to leptospira interrogans. the manner in which these persons acquire this organism in the absence of recognized occupational, recreational, or epidemic risk factors is not known.19968928985
immunochemical characteristics and localization on cells of protective antigen (pag) prepared from leptospira interrogans serovar lai.immuno-electron microscopic methods revealed that the protective antigen (pag) of leptospira interrogans serovar lai exists on the outer envelope sheathing the leptospiral cell body. pag lost its protective activity after treatment by hydrolysis with 2 m formic acid at 100 c for 2 hr, or oxidation with periodate at 4 c for 40 hr. the antigenic oligosaccharide fraction was further purified from the hydrolyzed pag by immunoaffinity column coupled with protective monoclonal antibody, lw2, and by ge ...19968934679
enzyme-linked immunosorbent assay and immunoblot analysis for detection of antibodies to borrelia burgdorferi in dogs. the impact of serum absorption with homologous and heterologous bacteriae.sera from 665 apparently healthy dogs were examined for antibodies to the lyme disease spirochete borrelia (b.) burgdorferi by using an elisa with a whole cell sonicate of b. burgdorferi sensu stricto reference strain b31 (atcc 35210) as antigen. to discover false positive reactions due to the unsatisfactory specificity of conventional enzyme-linked immunosorbent assays for b. burgdorferi, sera were absorbed in parallel with both b. burgdorferi and a heterologous sorbent consisting of whole cell ...19969054122
leptospirosis - an emerging pathogen in travel medicine: a review of its clinical manifestations and management.leptospirosis is a zoonosis caused by a spirochete, leptospira interrogans. the disease has a wide range of clinical manifestations, ranging from a mild febrile illness to classic weil's disease, which presents with jaundice, myalgia, acute renal failure, and tissue hemorrhage. transmission usually occurs from contact with infected water surfaces. recently, recreational activities such as hiking, swimming in lakes, biking through infected water, and hunting have been associated with the acquisit ...19969815435
the efficacy of a leptospira interrogans serovars pomona and copenhageni and l. borgpetersenii serovar hardjo vaccine in cattle.an experimental, trivalent, bovine, leptospiral vaccine, containing inactivated leptospira interrogans serovars pomona and copenhageni and l. borgpetersenii serovar hardjo hardjobovis, was developed. the experimental vaccine was shown to protect hamsters against virulent challenge with each of the component serovars. in a serological efficacy test in cattle, the experimental vaccine was compared for bioequivalence with a similar product, registered in new zealand for veterinary use. the experime ...199616031896
the epidemiology of leptospira interrogans serovar pomona in grower pig herds.an epidemiological study on four farms showed that leptospiral infection became apparent in piglets from 12 weeks of age. the intensity of leptospiruria was greatest in the first 3-4 weeks of infection and from then on declined and became intermittent. factors affecting the cultural and serological prevalence of infection of the piglets were found to be the standard of hygiene and variations in the titre of the dam. dams with high titres gave better protection to their young than did those with ...199516031793
leptospirosis in madras--a clinical and serological study.leptospirosis was confirmed by microscopic agglutination test (mat) and/or elisa in 57 patients admitted to the government general hospital, madras, india, during november and december of 1990 and 1991 with symptomatology suggestive of the disease. fifty (88%) of the 57 cases were males; the mean age of all the cases was 39.6 years (range 17-72). the main clinical features were: fever 100% jaundice 84%, myalgia 82%, acute renal failure 72% and conjunctival suffusion 58%. non-azotemic jaundice oc ...19958713215
use of nondenaturing silver-stained polyacrylamide gel analysis of polymerase chain reaction amplification products for the differential diagnosis of leptospira interrogans infection.a 285-bp dna fragment was amplified using the polymerase chain reaction from 38 leptospira serovars of six different genomic species. the fragments amplified exhibited differential mobilities on nondenaturing polyacrylamide gels resulting from sequence-dependent conformational alterations. leptospira interrogans serovars could be distinguished from those of other species on this basis.19958582141
[significance of human leptospirosis in mexico. detection of leptospira antibodies in a blood donor population].the presence of specific serum antibodies has been used as a diagnostic test for human leptospirosis. the presence of these antibodies in humans is indicative of an active natural infection. its detection after exposure denotes the presence of immunity. serum samples from 206 adult blood donors were analyzed with a microscopic agglutination assay against 7 serovars of leptospira interrogans. a total of 7% were positive with the following serovar distribution; shermani 53%, canicola 33%, pyrogens ...19958582567
[seroepidemiologic investigations in the alpine ibex (capra i. ibex) of piz albris in the canton of grigioni (switzerland)].sero-epidemiological investigations in wild animals may allow to assess distribution of selected pathogens that sometimes seem to be involved in sanitary interrelationships between wild and domestic ungulates sharing the same areas. serological studies were carried out to investigate the prevalence of antibody against 8 pathogens in alpine ibex of albris colony (grisons, switzerland). investigated sera came from 89 animals shot by gamekeepers in 1990-1991. antibody against smooth brucella, coxie ...19958584868
is1533-based pcr assay for identification of leptospira interrogans sensu lato serovars.a pcr-based assay was developed for typing l. interrogans sensu lato serovars. the assay is designed to exploit the presence of many copies of the leptospiral insertion sequence is1533 and is1533-like sequences present in the genomes of most leptospiral serovars. the pcr primers were designed to amplify dna of unknown sequence between closely placed is1533 or is1533-like sequences. amplification reactions primed with is1533-based primers generated products of different sizes. when few copies of ...19958586718
[leptospira infected rat population as probable cause of a fatal case of weil's disease].in the summer of 1992 a patient died of a leptospiral infection which he probably had contracted while he was swimming in an artificial lake in the region of tübingen/reutlingen. regarding the epidemiological role of leptospirosis the rodent population was investigated, because rats and mice were often seen in the surrounding area. 11 rats and 20 mice could be trapped. from their urine or kidneys two leptospiral serovars were isolated: serovar copenhageni from rattus norvegicus and serovar saxko ...19958593131
[construction of genomic library of l. interrogans serovar lai and preliminary study of recombinant plasmid pdc38].a genomic library, consisting of approximate 12 000 recombinants, has been constructed for the leptospira interrogans serovar lai in e. coli. using puc18 as the vector. hybridization analysis with the dna fragment containing ompl1 gene was performed and 10 positive clones were screened from the genomic library. one of the positive clones, designated pdc38, showed hybridization signal with the dna of 7 serovars 8 strains of pathogenic leptospires, but not with the dna of nonpathogenic leptospires ...19958732051
the hemorrhagic syndrome of leptospirosis: an experimental study in guinea pigs.the hemorrhagic syndrome of leptospirosis was studied in guinea pigs. the study correlates hematological, histopathological and immunohistochemical alterations in sixty animals inoculated by the intraperitoneal route with 1ml of the culture of virulent strain of leptospira interrogans serovar copenhageni. leptospirae antigens were detected by immunoperoxidase, chiefly in liver, kidney and heart muscle capillaries. possible pathogenic mechanisms responsible for hemorrhagic syndrome are discussed ...19957480909
is1500, an is3-like element from leptospira interrogans.copies of an insertion-sequence (is)-like element were isolated from two closely related serovars of leptospira interrogans sensu stricto. nucleotide sequence analysis of the 1236 bp element showed a characteristic is structure with terminal imperfect inverted repeats (irs) flanking a 1159 bp central region. this element was designated is1500. four open reading frames (orfa-orfd) were found in the central 'unique' region of is1500. similarities were detected between orfa and orfb and the putativ ...19957496528
leptospira icterohemorrhagiae and leptospire peptidolgycans induce endothelial cell adhesiveness for polymorphonuclear leukocytes.we have examined the effect of the virulent leptospira interrogans strain teramo, serotype icterohemorrhagiae, on the adherence of human neutrophilic polymorphonuclear leukocytes (pmn) to cultured human umbilical vein endothelial cells (hec). selective pretreatment of hec with intact or sonicated leptospires caused a dose- and time-dependent increase of hec-pmn adhesion (13.2% +/- 2.5% adherence to untreated hec versus 46.3% +/- 5.6% adherence to hec pretreated for 4 h with 10(8) intact leptospi ...19957542637
leptospira interrogans serovar sejroe infection in a group of laboratory dogs.interstitial nephritis was seen histologically in 19 (59%) out of 32 pure-breed beagle dogs (16 males and 16 females) subjected to standard safety tests. in these animals no clinical abnormalities were observed and all the tested parameters (haematology, biochemistry and urine analysis) were within the normal ranges. leptospiral antibody titres ranging from 1 : 100 to 1 : 6400, against a serovar (hardjo) belonging to the sejroe serogroup, were detected by the microscopic agglutination test (mat) ...19957564215
seroepidemiologic study of three zoonoses (leptospirosis, q fever, and tularemia) among trappers in québec, canada.this study was undertaken to evaluate the prevalence of antibodies against francisella tularensis, coxiella burnetii, and certain serovars of leptospira interrogans among trappers in québec, canada. muskrat trapping was identified as a risk factor for f. tularensis infection, whereas having a cat at home apparently protected trappers against infection by l. interrogans. high percentages of control sera were positive for antibodies against c. burnetii (15%) and l. interrogans (5%), most frequentl ...19957583933
association of leptospiral seroreactivity and breed with uveitis and blindness in horses: 372 cases (1986-1993).recurrent uveitis, a leading cause of blindness in horses, often develops as a sequela to systemic leptospirosis. over a 7-year period, 63 of 112 (56%) horses with uveitis were seropositive for leptospira interrogans serovar pomona, but only 23 of 260 (9%) horses without uveitis were seropositive. odds-ratio analysis revealed that seropositive horses were 13.2 times more likely to have uveitis than were seronegative horses. of the 63 seropositive horses with uveitis, 59% developed blindness, com ...19957591929
need for vaccination of sewer workers against leptospirosis and hepatitis a.this study compared the prevalence of leptospira interrogans and hepatitis a virus (hav) antibodies in serum samples from sewer workers and controls.19957663634
[a clinico-epidemiological study of leptospirosis in adults in the province of ciego de avila].a clinical epidemiological study of reported leptospirosis cases in adults in the period 1984-1988 in the province ciego de avila, republic of cuba, was conducted. the most frequent symptoms and signs were: fever, headache and arthralgia. eighty-two percent of patient were anicteric. the most frequent presumptive diagnosis included leptospirosis, virosis, and febrile syndrome. sporadic cases predominated over cluster cases. the incidence of cases was higher from october to december. sixty-five p ...19957667520
inhibition of na,k-atpase by an endotoxin extracted from leptospira interrogans: a possible mechanism for the physiopathology of leptospirosis.clinical manifestations of leptospirosis include disorders of the electrolytical balance which might be related to inhibition of na,k-atpase. although the physiopathological cellular mechanism of leptospirosis remains unknown, a bacterial endotoxin has been incriminated. therefore, we evaluated whether a glycolipoprotein fraction extracted from leptospira interrogans and known to be cytotoxic might inhibit na,k-atpase. this glycolipoprotein fraction (glp) inhibited na,k-atpase activity in rabbit ...19957671008
[scientific raisins from 125 years smw (swiss medical weekly). a brief report on the discovery of the pathogen (spirochaeta icterohaemorrhagiae nov. sp.) of so-called weil's disease in japan and on current studies of the disease. 1916]. 19957732354
recognition of leptospira interrogans antigens by vaccinated or infected dogs.antigenic recognition of leptospiral antigens by vaccinated or infected dogs was studied by microagglutination test (mat) and by western blots. in western blots, serovar specific antigens detected by mat migrated in the 18-31 kda zone. the 25-31 zone seemed to be linked to antigens indicating virulence of the strain. these antigens are lps. the first antibodies made after infection are produced against lps migrating in the 14 kda zone. many protein antigens are common in leptospires belonging to ...19947801528
protective effects of serum thymic factor to leptospira interrogans serovar copenhageni infection in mongolian gerbils.the susceptibility to leptospira interrogans serovar copenhageni in mongolian gerbils treated with 10 micrograms of serum thymic factor (fts) 1 day before infection was examined. susceptibility of gerbils treated 5 times with 10 micrograms of fts was also investigated. mortality of fts-treated gerbils was significantly lower than that of controls when small challenge doses were used. to analyse the fts-induced resistance to leptospiral infection, natural killer (nk) cell activity and macrophage ...19947801529
evaluation of an enzyme-linked immunosorbent assay that uses the 41-kd flagellin as the antigen for detection of antibodies to borrelia burgdorferi in cattle.an elisa was developed to detect antibodies to the 41-kd flagellin (p41) of borrelia burgdorferi in serum obtained from cattle. absorption studies, immunoblot analysis, immunoelectron microscopy, and correlation of results of the p41-elisa and the p39-elisa as well as measurement of the antibody to p41 in calves challenge-exposed with borrelia theileri were used to assess the specificity of the p41-elisa. antigens derived from escherichia coli, leptospira interrogans serovar hardjo, and b burgdo ...19947802386
sheep as maintenance host for leptospira interrogans serovar hardjo subtype hardjobovis.transmission of leptospira interrogans serovar hardjo subtype hardjobovis from naturally infected sheep to uninfected sheep and calves was studied. a microscopic agglutination test and elisa were used to determine specific antibody responses in serum. polymerase chain reaction was used to detect bacterial shedding in urine. six sheep were derived from a dairy farm where cows were infected with l hardjobovis. three of these sheep were seropositive for l hardjobovis, and 1 also shed leptospires in ...19947802389
a review of laboratory techniques and their use in the diagnosis of leptospira interrogans serovar hardjo infection in cattle.this paper reviews the laboratory diagnosis of leptospira hardjo infection in cattle. two genotypes of l hardjo, hardjoprajitno and hardjobovis, have been identified in cattle, but only hardjobovis has been isolated in australia. there are problems with diagnosis and control of bovine leptospirosis. infection is usually subclinical and the serological titres vary greatly in peak and duration. leptospires may be excreted in urine for up to 18 months. low microscopic agglutination test titres may ...19947818437
rapid and specific detection of pathogenic leptospira species by amplification of ribosomal sequences.we have developed an assay for the detection of pathogenic leptospira that is based on the polymerase chain reaction. with the combination of agarose gel electrophoresis and blotting, pathogenic leptospira can be discriminated specifically from nonpathogenic leptospira and other bacterial species. this method, based on the amplification of 16s ribosomal rna sequences, is able to detect 10 leptospiral cells/ml in cattle urine samples and 100 leptospiral cells/ml in pig urine samples. using this a ...19947866864
lyme disease spirochetes in a wild fox (vulpes vulpes schrencki) and in ticks.lyme disease spirochetes were demonstrated in a wild female fox (vulpes vulpes schrencki) and in ixodes persulcatus ticks collected from the fox on sapporo, hokkaido, japan. spirochetes were detected in i. persulcatus, as well as skin lesions, brain, heart, kidney, and liver of the fox. five of seven isolates reacted with a monoclonal antibody against borrelia afzelii specific osp b. deoxyribonucleic acid (dna) relatedness of a brain isolate was 89% to b. afzelii, and ranged from 50 to 67% to th ...19947933292
seroprevalence to leptospira interrogans serovar hardjo in merino stud rams in south australia.a serological survey of 2160 merino stud rams on 36 farms detected positive reactions greater than or equal to 1/100 in 42% of animals using the microscopic agglutination test (mat) to leptospira interrogans serovar hardjo. twenty flocks had seroprevalence values greater than 30% with 15 flocks having values > or = 60%. the enzyme-linked immunosorbent assays showed that 47% and 3% of rams on the 36 farms were positive for igg and igm antibodies, respectively. forty-five percent of hardjo reactio ...19947945098
survey of antibodies against various infectious disease agents in racing camels in abu dhabi, united arab emirates.prevalence of antibodies against some important disease agents in sera from racing camels in abu dhabi (united arab emirates) is reported. antibodies against brucella abortus were detected in 1.5% of racing camels, but only 0.76% had titres sufficient for the animals to be considered infected. the complement fixation test revealed antibodies against coxiella burnetii (causative agent of q fever) in 7.9% of camels (with a geometrical mean titre of 13) and against parainfluenza virus type 3 in 5.6 ...19947949353
characterization of an antigenic oligosaccharide from leptospira interrogans serovar pomona and its role in immunity.an antigenic oligosaccharide fraction derived from the lipopolysaccharide of leptospira interrogans serovar pomona was isolated by endo-glycosidase h digestion and column chromatography. the oligosaccharide contained rhamnose, ribose, glucose, and glucosamine and inhibited the binding of opsonic, protective monoclonal antibodies directed against the lipopolysaccharide. when conjugated to diphtheria toxoid, the oligosaccharide elicited the production of agglutinating, opsonic antibodies.19947960129
return to oestrus after first insemination in sow herds (incidence, seasonality, and association with reproductivity and some blood parameters).as no systematic study has been done to get an accurate estimate of the incidence of return to oestrus after first insemination in sows in the netherlands, the objectives of this investigation were: 1) to obtain an estimate of the incidence of return to oestrus after insemination at the herd level; 2) to investigate the association between incidence of return to oestrus after first insemination and reproduction characteristics in order to get an impression of the economic importance of reproduct ...19947985351
decreased erythrocyte osmotic fragility during canine leptospirosis.erythrocyte osmotic fragility (eof) was carried out in nineteen dogs naturally infected by leptospira interrogans serovar icterohaemorrhagiae/copenhagi. a decreased eof was observed, suggesting a modification of erythrocyte components secondary to disturbances that occur during canine leptospirosis, such as renal damage and hepatic disease.19947997768
seroprevalence of leptospirosis in a rural flood prone district of bangladesh.leptospirosis is a worldwide zoonotic disease. in the present investigation, a total of 89 human sera from a flood prone district of bangladesh was screened by a one-point microscapsule agglutination test (mcat). mcat-positive and -doubtful sera were further tested by microscopic agglutination test (mat) against 16 reference serovars of leptospira interrogans, and the antibody titres determined. in mcat, 34 sera were positive and 22 were doubtful. among those positive and doubtful sera, 33 and 2 ...19948005218
leptospirosis patient with aids: the first case reported.a case of renal icterohemorrhagic leptospirosis involving a patient with acquired immunodeficiency syndrome (aids) is reported. despite the low levels of cd4+ t lymphocytes, the clinical course of leptospirosis was similar to that observed in non-immunodepressed patients, and no worsening of aids occurred due to the infection by the spirochete. serologic conversion was observed in the microscopic agglutination test, with maximum titer of 1:3,200. the patient had positive urine cultures for lepto ...19948008919
[development of a test system for detecting leptospira interrogans using the polymerase chain reaction].based on polymerase chain reaction a test-system has been elaborated permitting one to identify the leptospirae of the most common serogroups (icterohaemorrhagiae, canicola, javanica, ballum, pyrogenes, pomona, habdomadis, sejroe, tarassovi) of the species leptospira interrogans. sensitivity of the technique is 1-10 cells in a sample. the specificity of the system has been shown to depend on the temperature of the primers annealing. the elaborated system exceeds all other systems for leptospiral ...19948133844
[a polymerase chain reaction method for studying host persistence of pathogenic leptospira].polymerase chain reaction has for the first time been shown to be applicable to indication of leptospira interrogans in the organs of infected animals with acute or chronic leptospirosis (on the model of golden syrian hamsters). polymerase chain reaction is superior to microscopic and bacteriological analyses in identification of leptospirae in organ suspensions. the sensitivity of the technique is 1-10 cells per sample in studies of kidney or brain suspensions or 100-1000 cells in studies of li ...19948133845
immunochemical studies of opsonic epitopes of the lipopolysaccharide of leptospira interrogans serovar hardjo.leptospiral lipopolysaccharides (lps) are the main antigens responsible for immunity in leptospirosis. in this investigation we studied the nature of the antigenic determinants of lps extracted from leptospira interrogans serovar hardjo (reference strain hardjoprajitno). the reactions of anti-lps monoclonal antibodies (mabs) mum/f1-4/hardjo (igm) and mum/f1-6/hardjo (igg) with whole cell lysates in western immunoblotting analysis were unaffected by proteinase k treatment. periodate treatment of ...19947513591
effective treatment with dihydrostreptomycin of naturally infected cows shedding leptospira interrogans serovar hardjo subtype hardjobovis.the efficacy of dihydrostreptomycin in stopping the shedding of leptospira hardjo subtype hardjobovis was studied in naturally infected cows. blood and urine samples were collected from dairy cows kept on a farm where the farmer had contracted l hardjobovis infection. a microscopic agglutination test and an elisa were used to determine specific antibody responses in serum. polymerase chain reaction was used to detect bacterial shedding in urine. on the first sample collection date, 6 cows were s ...19947514850
a neutral sugar is responsible for serovar specificity of the antigenic determinant of leptospira interrogans serovar canicola.to provide information on the chemical structures of antigenic determinants of leptospira, glycolipids of leptospira interrogans serovar canicola strain hond utrecht iv (ut-iv) and its antigenic variant selected in the presence of a serovar-specific monoclonal antibody were compared physicochemically. gas-liquid chromatography-mass spectrometry analysis revealed that the glycolipid of ut-iv contained 6 neutral sugar species; rhamnose, mannose, galactose, glucose, and unknown sugars iii and iv, i ...19947528287
[16s rrna reverse transcription-polymerase chain reaction of leptospires].we amplified the leptospiral rnas of leptospira interrogans serovar lai strain lai and l. biflexa serovar patoc strain patoc i, extracted by the method of sio2-high concentration salt solution (8 mol/l guhcl) absorption, with the 16s rrna gene primers of leptospira interrogans by reverse transcription-polymerase chain reaction (rt-pcr). it demonstrated that the detecting sensitivity by naked eye after electrophoresis could be 100 times higher when we amplified dna and rna of leptospires at the s ...19947528714
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