Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| demonstration of immunoglobulin containing deposits in glomerular basement membrane in experimental chronic serum sickness using horseradish peroxidase labelled antiserum. | horseradish peroxidase (hrp) labelled antibodies were used in rabbit glomerulonephritis to obtain localization of immunoglobulins in the glomerulus at a light and electron microscope level. better localization of deposits was obtained with peroxidase labelled antiserum than with immunofluorescence at the light microscope level. ultrastructurally, immunoglobulin localization was seen to correspond to the electron dense deposits demonstrated in and on the glomerular basement membrane by convention ... | 1975 | 1106748 |
| mesencephalic porjections of the rectus lateralis muscle afferents in the cat. | responses to passive stretch applied to the rectus lateralis muscle (rl) were recorded with a microelectrode from the mesencephalic nucleus of the fifth nerve (mes v) in cat encéphale isolé preparation. the necessary conditions to attribute the unit response to an eye muscle stretch are the followings: i) respond neither to jaw movements nor to pressure applied to the eye ball: ii) be activated with a short latency, following stretch of the eye muscles; iii) be excited by a light touch applied t ... | 1975 | 1080407 |
| trigeminal representations of the masticatory and extraocular proprioceptors as revealed by horseradish peroxidase retrograde transport. | horseradish peroxidase has been injected in the masticatory and extraocular muscles in newborn and adult cats to identify the cells of origin for the muscle endings. labeled motoneurons in the nuclei of the iii, iv, v and vi nerves have been observed. they are the parent cells of the motor terminals taking up the enzyme in the muscle injected. labeled ganglionic cells have been found scattered all along the ipsilateral mesencephalic nucleus of the v nerve after injection of both the jaw closing ... | 1975 | 1081052 |
| morphology of the breakdown of the blood-aqueous barrier in the ciliary processes of the rabbit eye after prostaglandin e2. | protaglandin e2, administered topically to the rabbit eye, causes disruption of the blood-aqueous barrier resulting in a large increase in the protein content of the aqueous humor. the route of plasma proteins into the aqueous humor was studied with the electron microscope, using horseradish peroxidase as a protein tracer. the tracer penetrated the tight junctions of the nonpigmented layer of the ciliary epithelium, filling the lateral intercellular clefts and staining the internal limiting mem ... | 1975 | 1110133 |
| chorioretinal diffusion processes following pigment epithelial degeneration. | we studied the effect of sodium iodate-induced pigment epithelial degeneration on chorioretinal diffusion processes. rabbits received two intravenous injections of sodium iodate at the retinotoxic dose of 22.5 mg. per kilogram over a six- to eight-hour period. combined horseradish peroxidase tracing technique and electron microscopy were used to compare diffusion processes over a five-week period. ultrastructural examination of retina twenty-four hours after iodate administration revealed pigmen ... | 1975 | 1110139 |
| subcortical afferents to the frontal lobe in the rhesus monkey studied by means of retrograde horseradish peroxidase transport. | 1975 | 1111842 | |
| a combined horseradish peroxidase-autoradiographic investigation of reciprocal connections between superior temporal gyrus and pulvinar in squirrel monkey. | 1975 | 1111845 | |
| permeability properties of the postcapillary high-endothelial venules in lymph nodes of the mouse. | the permeability of the postcapillary high-endothelial venules in mice was investigated by light and electron microscopy. experiments with intravenously injected horseradish peroxidase demonstrated a pronounced intercellular passage of this tracer. vesicular transport was not observed. additional experiments with lanthanum and tannic acid showed that no continous tight junctions sealed the intercellular spaces. however, some focal membrane appositions were observed. | 1975 | 1113511 |
| basal forebrain and hypothalamic connection to frontal and parietal cortex in the rhesus monkey. | horseradish peroxidase was injected in different parts of the frontal and parietal cortex in 17 rhesus monkeys. in all cases the enzyme was transported retrogradely to neurons in the substantia innominata and hypothalamus as well as in the thalamus. these new findings demonstrate that these cortical areas receive direct afferent fibers from limbic basal forebrain areas concerned with emotion and motivation. | 1975 | 1114317 |
| acquisition of host antigens by young schistosoma mansoni in mice: correlation with failure to bind antibody in vitro. | young schistosomes collected after penetration through isolated mouse skin (3 h schistosomula) were cultured in medium containing immune rhesus monkey serum with a high titre of antibody known to kill schistosomula in the presence of complement. morphological signs of damage in electron micrographs were confined to the surface tegument of the schistosomula. antibodies in immune rhesus serum were shown to bind to the surface membrane of 3 h schistosomula using an antibody-enzyme bridge technique ... | 1975 | 1118189 |
| permeability of ovarian follicles and capillaries in mice. | the permeability of ovarian capillaries and follicles in prepubertal and sexually mature (proestrus and metestrus) randomly bred swiss albino female mice (sch:ars ha icr strain) was studied by intravenous injection of either ferritin or horseradish peroxidase (hrp), followed by examination with light and electron microscopes. the study revealed that capillaries in the interstitial and perifollicular regions were provided with a continuous endothelium that had constant permeability characteristic ... | 1975 | 1119413 |
| increased penetration of horseradish peroxidase across the blood-brain barrier induced by metrazol seizures. | 1975 | 1122393 | |
| lymphocyte differentiation and antibody synthesis in the secondary immune response of peroxidase stimulated lymph nodes of rat. | lymphocyte differentiation and specific antibody synthesis were studied in the course of the secondary immune response of horseradish-peroxidase immunized rat popliteal lymph nodes by electron microscopic immunocytochemistry. from primary immunization, mature plasma cells containing specific antibody remained as long as 10 months. antihorseradish peroxidase antibodies were localized in lymphocytic blast cells on day 3 after restimulation with antigen. in the subsequent days blast cells were stil ... | 1975 | 1122527 |
| an ultrastructural quantitative method for the evaluation of the permeability to horseradish peroxidase of cerebral cortex endothelial cells of the rat. | a quantitative method for the evaluation of the importance of pinocytotic phenomena in cerebral cortex capillaries is proposed. the method involved the counting of labeled "caveolae intracellulares" in endothelia cells with the use of horseradish peroxidase as a tracer molecule. a mean pinocytotic value per capillary is then computed. it is demonstrated that the value obtained after irradiation of the brain is higher than in control animals. this information may provide an ultrastructural quanti ... | 1975 | 1122535 |
| h2o2 release from human granulocytes during phagocytosis. i. documentation, quantitation, and some regulating factors. | the extinction of fluorescence of scopoletin during its oxidation by horseradish peroxidase (hpo) provides a highly sensitive and specific assay for small quantities of peroxide in solution. with this assay, the release of free h2o2 into the extracellular medium by phagocytizing human granulocytes has been documented and quantitated, and some of the regulating factors have been determined. under basal conditions granulocytes released less than 0.01 nmol/ml of h2o2 (2.5 x 10-6 polymorphonuclear l ... | 1975 | 1123431 |
| application of immunoperoxidase techniques to localize horseradish peroxidase-tracer in the central nervous system. | immunoperoxidase techniques are presented which can be used to localize horseradish peroxidase-tracer in paraffin-embedded tissues of the central nervous system. compared to histochemical methods using frozen sections, these immunologic techniques allow the use of stored, serial paraffin sections, and appear more sensitive for the demonstration of intraneuronal horseradish peroxidase after retrograde transport. the immunoperoxidase bridge techniques from reaction products of high quality which c ... | 1975 | 1127219 |
| sympathetic preganglionic neurons of the cat spinal cord: horseradish peroxidase study. | 1975 | 1131693 | |
| diffusion of horseradish peroxidase perfused through the lateral ventricle of the chick telencephalon. | horseradish peroxidase, perfused into the lateral ventricle of chick brain, freely and slowly diffuses through the cerebral extracellular spaces. the layer of astrocytic end-feet surrounding blood capillaries does not consitute a barrier to the tracer which permeates the basal lamina, diffuses between the pericytic cells and finally accumulates in the intercellular space beneath the tight junctions between contiguous endothelial cells. no evidence was found for transport by micropinocytotic vesi ... | 1975 | 1131857 |
| effects of contrast agents on the blood-brain barrier. an electron microscopic study. | to determine if contrast agents after the blood-brain barrier by opening tight junctions between endothelial cells and by increasing pinocytosis, the distribution of horseradish peroxidase (hrp) was evaluated after intracarotid injections of contrast media. hrp did not cross the endothelium of cerebral blood vessels. tight junctions remained closed. there was increased endothelial cell pinocytosis and swelling of astrocyte end-feet. | 1975 | 1138267 |
| the effect of portocaval anastomosis on the permeability to horseradish peroxidase of cerebral vessels of the rat. | the blood-brain barrier to horseradish peroxidase (hrp) was tested in rats with hepatic encephalopathy induced by portocaval anastomosis. an increased number of vesicles containing hrp was seen in endothelial cells. basement membranes of some vessels and the adjacent neuropil were filled with hrp. astrocytes showed swollen end-feet. the cytoplasm of astrocytes adjacent to hrp containing basement membranes was evenly filled with hrp. this phenomenon could be caused either by cellular hyperactivit ... | 1975 | 1146553 |
| some aspects of the organization of the thalamic reticular complex. | anatomical methods which depend upon the anterograde axonal transport of isotopically labeled neuronal proteins or the retrograde axonal transport of the enzyme, horseradish peroxidase, have been used to elucidate the relationships between the reticular complex and the dorsal thalamus and cerebral cortex. injections of tritiated amino acids in the dorsal thalamus or cerebral cortex in rats, cats and monkeys, show that as the bundles of thalamo-cortical and cortico-thalamic fibers joining a parti ... | 1975 | 1150923 |
| pathogenesis of acute arterial fat deposition in spontaneously hypertensive rats. | the selectively-bred substrains of spontaneously hypertensive rats with a greater vulnerability to vascular lesions rapidly developed arterial fat deposition within 1 or 2 weeks as well as a greater hypercholesterolemic response when fed on high fat cholesterol diet including 20% of suet, 5% of cholesterol and 2% of cholic acid. the ring-like arterial fat deposition at the branches of superior mesenteric arteries and cerebrobasal arteries, which was found to be good indices for the deposition of ... | 1975 | 1152192 |
| evidence for bidirectional dendritic transport of horseradish peroxidase. | 1975 | 1155260 | |
| observations on cortical neurons retrogradely labeled with horseradish peroxidase. | 1975 | 1155261 | |
| the appearance of dendrites of callosal and corticothalamic neurons in somatosensory cortex of immature rats demonstrated by horseradish peroxidase. | 1975 | 1155262 | |
| absorption of intact protein by colonic epithelial cells of the rat. | colonic absorption of intact protein was examined in adult rats using histological and ultrastructural procedures. horseradish peroxidase was introduced into ligated colonic loops and retained therein for 5, 10, 20, or 30 minutes prior to excision of the loops, and their processing for microscopy. morphological evaluation revealed evidence of peroxidase absorption via pinocytosis. tracer particles were observed adherent to the mucosal border, in apical pinocytotic vesicles, in vesicles adjacent ... | 1975 | 1155414 |
| demonstration of a somatotopically organized projection onto the paramedian lobule and the anterior lobe from the lateral reticular nucleus: an experimental study with the horseradish peroxidase method. | using the retrograde axonal transport of horseradish peroxidase, the projection from the lateral reticular nucleus (nrl) to the cerebellar anterior lobe and paramedian lobule has been studied in 13 cats. both the anterior lobe and the paramedian lobule receive a somatotopically organized projection from the nrl. the projection to the paramedian lobule is nearly exclusively ipsilateral and originates mainly in the dorsal part of nrl, while the projection to the anterior lobe is bilateral (with ip ... | 1975 | 1156873 |
| the pontocerebellar projection onto the paramedian lobule in the cat: an experimental study with the use of horseradish peroxidase as a tracer. | horseradish peroxidase (hrp) was injected into cerebellar cortex of the paramedian lobule in 12 cats, and the ensuing distribution of labeled cells in the pontine nuclei was mapped in some detail. the cells in the pontine gray which give origin to fibers to the paramedian lobule lie together, in part in groups, and in part in columns. the columns are situated both medial and ventrolateral to the peduncle, as well as in the dorsolateral pontine nucleus. the projection is bilateral with a clearcut ... | 1975 | 1156877 |
| the projections of different morphological types of ganglion cells in the cat retina. | the central projections of the retinal ganglion cells of the cat were examined using the method of retrograde transport of horseradish peroxidase. peroxidase was injected into the lateral geniculate nucleus and into the superior colliculus by means of a recording micropipette. after injections at retinotopically homologous points in these two structures in separate animals, tha patterns of retinal ganglion cell labeling were compared. we found that there were three populations of ganglion cells: ... | 1975 | 1159111 |
| the projections of cells in different layers of the cat's visual cortex. | the projection of cells in different layers of several cortical visual areas in the cat were studied using the method of retrograde transport of horseradish peroxidase. injections of the enzyme were made through a recording micropipette, making it possible to localize the injection site by physiological criteria. we found that layer vi cells projected to the alteral geniculate nucleus, while a distinct population of cells in layer v projected to the superior colliculus. cells in layers ii and ii ... | 1975 | 1159112 |
| studies on the structure and permeability of the microvasculature in normal rat lymph nodes. | the structure and permeability of the microvasculature in normal rat lymph nodes was studied by regional perfusion techniques. the results indicated that characteristic vascular units supplied each cortical lobule of lymphatic tissue. numerous arteriovenous communications and venous sphincters innervated by unmyelinated nerve fibers were found in this vascular bed. these specialized vascular structures permitted regional control of blood flow through high endothelial venules. lymphocytes migrate ... | 1975 | 1163637 |
| impermeability of sertoli cell junctions to prolonged exposure to peroxidase. | the competence of the occluding junctional complexes between sertoli cell has been studied after continuous exposure of the testis to a high concentration of peroxidase for periods of 30 minutes to 4 hours. horseradish peroxidase in buffered saline solution (10 mg/ml) infused through an indwelling polyethelene tube implanted interstitially in the testis becomes widely distributed throughout the organ via the extensive system of lymphatic sinusoids. all the components of the testicular interstiti ... | 1975 | 1163825 |
| macromolecular transport by the middle ear and its lymphatic system. | the macromolecular transport in the middle ear mucosa of the guinea pig was investigated by means of light and electron microscopy using tracer substances such as evans blue, india ink, and horseradish peroxidase (hrp). hrp particles were readily taken up by the middle ear mucosa and eustachian tube. they were absorbed by all cell types, including ciliated, secretory and nonsecretory cells. the particles were first taken up by the pinocytotic vesicles and then transported into the intercellular ... | 1975 | 1166775 |
| immunoelectron microscopic identification and localization of streptococcus sanguis with peroxidase-labeled antibody: localization of streptococcus sanguis in intact dental plaque. | streptococcus sanguis has been localized ultrastructurally within intact dental plaque by means of an indirect technique which utilizes horseradish peroxidase-labeled antibody. the technique allows for complete diffusion of the reagents to all portions of the plaque specimens. control procedures can be carried out on serial sections of plaque with a bacterial composition similar to that of the experimental specimen. the 30-mum-thick sections can be examined in the light microscope to localize ar ... | 1975 | 1167846 |
| endogenous peroxidase: specific marker enzyme for tissues displaying growth dependency on estrogen. | data derived from a correlated morphological and biochemical study suggest the following: (a) estradiol-17beta, diethylstilbestrol, the estrogen antagonists nafoxidine (upjohn 11,000), and parke davis c1628 induce synthesis of an endogenous peroxidase in the epithelium of target tissues like the vagina, the cervix, the uterus, and in the acinar cells of the estrogen-dependent rat mammary tumor; (b) peroxidase is a "specific" secretory protein of the estrogen-sensitized uterine endometrium; (c) p ... | 1975 | 1171106 |
| permeability of rabbit corneal epithelium to horseradish peroxidase after the influence of benzalkonium chloride. | a tight barrier against permeation of horseradish peroxidase into the corneal epithelium exists at the corneal surface adjacent to the tear film. the present light and transmission electron microscopic study reveals that the cationic surfactant, benzalkonium chloride, which is commonly added to eye drops as a preservative, breaks down this barrier. lysis of the cell membranes was demonstrated, resulting in a leakage of the tracer into and underneath the superficial cells. the grade of cellular d ... | 1975 | 1174396 |
| magnocellular nuclei of the basal forebrain project to neocortex, brain stem, and olfactory bulb. review of some functional correlates. | horseradish peroxidase was injected into the neocortex of squirrel monkeys, rats, tree shrews and one opossum, in the brain stem of one squirrel monkey and rats, and in the olfactory bulb, the corpus vitreum or the vascular system of rats. following the cortical, brain stem and bulbar injections labeled cells were found (predominatly ipsilaterally) in the magnocellular nuclei of the basal forebrain: nucleus of the diagonal band, the magnocellular preoptic nucleus and nucleus basalis. these nucle ... | 1975 | 1174977 |
| delineation of dorsal lateral geniculate afferents from the cat brain stem as visualized by the horseradish peroxidase technique. | 1975 | 1174981 | |
| different targets of different neurons in nucleus gracilis of the cat. | efferent projections of neurons in the cat nucleus gracilis to the ventrobasal complex (vb) of the thalamus and the dorsal accessory portion of the inferior olive (iod) were studied using tracing techniques that exploit neuronal orthograde and retrograde intra-axonal transport processes. these projections were studied in order to determine if the heterogeneity of the morphology, physiology and afferent input of the nucleus gracilis is paralleled by a similar heterogeneity in its efferent project ... | 1975 | 1176641 |
| localization of masticatory motoneurons in the cat and rat by means of retrograde axonal transport of horseradish peroxidase. | topographical localization of monotoneurons supplying the masticatory muscles was investigated in the cat and rat, utilizing retrograde axonal transport of horseradish peroxidase. following injection of horseradish peroxidase in each masticatory muscle, motoneurons labelled with peroxidase were seen to be aggregated into a cluster within the motor nucleus of the trigeminal nerve. such clusters of peroxidase-motoneurons innervating each masticatory muscle were demarcated more sharply in kittens t ... | 1975 | 1176649 |
| ultrastructural studies on cerebrovascular permeability in acute hypertension. | acute hypertension, experimentally induced by intravenous injection of metaraminol in adult rabbits, rapidly induced a damage of the blood-brain barrier in the cerebral cortex, as visualized by evans-blue-conjugated albumin and horseradish peroxidase. extravasation of these two exogenous tracers was demonstrated to occur in arterioles, in capillaries and, rarely, in venules. peroxidase passed the endothelial cell into the nervous tissue in either or three different ways, i.e. through channels, o ... | 1975 | 1180001 |
| mössbauer investigations of high-spin ferrous heme proteins. i. cytochrome p-450. | anaerobically reduced samples of cytochrome p-450 from pseudomonas putida were studied by mössbauer spectroscopy. in the presence of an applied magnetic field the high-spin ferrous heme iron showed an intricate pattern of electric and magnetic hyperfine interactions which could be parametrized successfully in terms of a spin hamiltonian formalism. the results imply a very low (triclinic) symmetry of the heme iron. the effects of the ligand environment and of spin-orbit coupling result in a large ... | 1975 | 1182094 |
| mössbauer investigations of high-spin ferrous heme proteins. ii. chloroperoxidase, horseradish peroxidase, and hemoglobin. | reduced samples of chloroperoxidase, horseradish peroxidase, and deoxyhemoglobin were studied by mössbauer spectroscopy in strong magnetic fields. the intricate paramagnetic spectra of chloroperoxidase were evaluated in detail in the framework of a spin hamiltonian pertinent to high-spin ferrous iron. the studies strongly suggest that, in their reduced states, chloroperoxidase from caldariomyces fumago and cytochrome p-450 from pseudomonas putida have similar, if not identical ligand structures ... | 1975 | 1182095 |
| vasogenic edema in the injured spinal cord: a method of evaluating the extent of blood-brain barrier alteration to horseradish peroxidase. | 1975 | 1183533 | |
| proceedings: a quantitative study of horseradish peroxidase uptake by boutons presynaptic to axotomized hypoglossal neurones. | 1975 | 1185647 | |
| location of cat trigeminal ganglion cells innervating dental pulp of upper and lower canines studied by retrograde transport of horseradish peroxidase. | 1975 | 1188971 | |
| the central cervical nucleus as cell origin of a spinocerebellar tract arising from the cervical cord: a study in the cat using horseradish peroxidase. | 1975 | 1192184 | |
| cell populations of the lateral geniculate nucleus of the cat as determined with horseradish peroxidase. | 1975 | 1192198 | |
| localization of the efferent neurons of the tensor tympani muscle of the newborn kitten using horseradish peroxidase. | 1975 | 1193198 | |
| altered reabsorption of protein by the renal cortex in rats treated with hypertonic saline or mannitol. | the reabsorption of horseradish peroxidase (hrp) by the proximal tubule cells of rat kidneys was investigated by measuring the concentration of hrp in total particulate fractions of the cortex 1/4 and 1 hr after intravenous injection, and by correlated cytochemical observations. when compared to the corresponding values of the control animals, the concentration of hrp 1 hr after injection was decreased approximately 10-fold in the renal cortex of rats which had received an intravenous injection ... | 1975 | 1194661 |
| membrane permeability alterations as manifestation of early cardiac muscle cell injury. | studies with extracellular fine structural diffusion protein tracers, horseradish peroxidase and ferritin, were carried out on catecholamine-induced cardiac muscle cell injury. epinephrine, norepinephrine, and isoproterenol were administered in a dose of 4- 6 mug/100g body weight by continuous intravenous infusion. for follow-up studies, isoproterenol was also given subcutaneously in a dose of 8.5 mg/100 g body weight as a single injection. in contrast to saline-infused controls and following ep ... | 1975 | 1197896 |
| changes in vascular permeability in stroke-prone spontaneously hypertensive rats studied with peroxidase as a tracer. | cerebrovascular permeability in stroke-prone spontaneously hypertensive rats (shr) at various ages was histologically studied using horseradish peroxidase as a tracer and such was related to the cerebrovascular lesions in the animals. an increase in permeability was demonstrated in the brain of shr, particularly in those animals with an extremely high blood pressure. increased cerebrovascular permeability occurred in some animals without any organic vascular change or severe parenchymal changes, ... | 1975 | 1199727 |
| structural changes in the membrane of vero cells infected with a paramyxovirus. | vero cells productively infected with the halle strain of measles virus have been studied by means of surface replication, freeze-fracturing, and surface labeling with horseradish peroxidase-measles antibody conjugate in order to examine changes in the structure of the cell membrane during viral maturation. early in infection, the surfaces of infected cells are embossed by scattered groups of twisted strands, and diffuse patches of label for viral antigens cover regions marked by these strands. ... | 1975 | 1202014 |
| an ultrastructural study of normal and injured hypoglossal nuclei after injection of horseradish peroxidase. | the left hypoglossal nuclei of normal rats, and rats which had received left hypoglossal nerve axotomies 7-21 days previously, were studied by electron microscopy after injection of horseradish peroxidase as a marker of extracellular space and pinocytosis. quantitative analysis showed that the number of pinocytotically-derived structures in presynaptic boutons was significantly increased in rats at 7, 14, and 21 days after axotomy when compared with normal rats. it was suggested that presynaptic ... | 1975 | 1204692 |
| [evidence for direct projections from the hypothalamus to the lateral geniculate and occipital cortex of the cat as demonstrated by horseradish peroxidase technique (author's transl)]. | 1975 | 1204798 | |
| olfactory relationships of the telencephalon and diencephalon in the rabbit. ii. an autoradiographic and horseradish peroxidase study of the efferent connections of the anterior olfactory nucleus. | the efferent connections of the anterior olfactory nucleus in the female albino rabbit have been studied using the autoradiographic and horseradish peroxidase methods for tracing axonal pathways. following a unilateral injection of 3h-leucine into the olfactory peduncle, radioactively labeled efferent projections from the anterior olfactory nucleus were traced into all layers of the ipsilateral main olfactory bulb beneath the olfactory nerve layer and through the ipsilateral anterior limb of the ... | 1975 | 1206126 |
| the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. | the distribution of labeled cells in the inferior olive of the cat has been mapped following injections of small amounts of horseradish perosidase in the paramedian lobule of the cerebellum. the distribution of labeled cells was plotted in drawings of approximately serial transverse sections. the findings in each case were transferred to a standard diagram of the olive to facilitate comparison of cases. previous studies of the distribution of retrograde cell loss in the inferior olive following ... | 1975 | 1206129 |
| functional and structural changes in mammalian sympathetic neurones following interruption of their axons. | the effects of interrupting the axons of principal neurones in the superior cervical ganglion of adult guinea-pigs were studied by means of intracellular recording, and light and electron microscopy. 1. within 72 hr of axon interruption, the amplitude of exitatory postsynaptic potentials potentials (e.p.s.p.s) recorded in principal neurons in response to maximal preganglionic stimulation declined. e.p.s.p.s were maximally reduced (by more than 70% on average) 4-7 days following interruption, and ... | 1975 | 1206535 |
| contractile apparatus in aortic endothelium of hypertensive rat. | one week after hypertension was produced in male wistar rats (150 g body weight) by a complete aortic ligature placed between renal arteries, electron microscopic studies showed a striking increase of cytoplasmic microfilaments in the endothelial cells of the aortic segment above coarctation (mean blood pressure 160 mm hg). these microfilaments measured 40-70 a in diameter and were located particularly close to the endothelial clefts. in "en face" preparation of aortic endothelial cells treated ... | 1975 | 1209006 |
| acute lead encephalopathy in the guinea pig. | acute lead encephalopathy was induced in adult guinea pigs with daily oral doses of lead carbonate. cerebral capillaries were examined by electron microscopy, and the blood-brain barrier (b-bb) evaluated with evans blue and horseradish peroxidase. brain lead levels were also determined during the developing encephalopathy. there was no cerebral capillary alteration or demonstrable b-bb dysfunction. brain lead concentrations increased over the 5-day period. the encephalopathy in the absence of an ... | 1975 | 1211109 |
| some enzymes present in the walls of mesophyll cells of tobacco leaves. | cell-wall enzymes were assayed by the difference between enzyme activities in the whole cell and the protoplast. both peroxidase (85.2%) and acid phosphatase (21.9%) were located in the wall. however, malate dehydrogenase was found only in the protoplast. a study of the time-course of the release of peroxidase and malate dehydrogenase into the incubation medium from cells either treated with cellulase or untreated, also indicated that peroxidase and not malate dehydrogenase was located in the wa ... | 1975 | 1212211 |
| rabbit homocytotropic antibody response to horseradish peroxidase: the role of contaminating antigens. | 1975 | 1218893 | |
| electron microscopic study on permeability of coronary artery wall of normotensive rabbits using horseradish peroxidase as a tracer. | in order to study the pathways in the coronary artery vessel wall horseradish peroxidase (hrp) was used as a tracer. hrp was injected directly in the left ventricle of rabbit hearts. 30 seconds after intracardial injection of hrp in normotensive rabbits, peroxidase reaction products could be located in random areas of the coronary arterial system and could then be observed in all three layers of the coronary walls at these locations. hrp reaches the subendothelial space of the coronary arteries ... | 1975 | 1220671 |
| blood-brain barrier dysfunction in acute lead encephalopathy: a reappraisal. | acute lead encephalopathy was induced in adult guinea pigs by administering daily oral doses of lead carbonate. during the development of the encephalopathy, the structural and functional integrity of the blood-brain barrier was evaluated with electron microscopy and tracer probes. blood, cerebral gray matter, liver, and kidney were analyzed for lead, calcium, and magnesium content. the animals regularly developed an encephalopathy after four doses of lead. there were no discernible pathomorphol ... | 1975 | 1227864 |
| absorption of horseradish peroxidase by the small intestinal epithelium in postnatal developing rats. | after an intraluminal injection of horseradish peroxidase into the small intestine, the localization of peroxidase was studied in neonatal developing and adult rats by means of electron microscopy. until around the 14th day of the neonatal period absorbed peroxidase granules in the duodenal and jejunal epithelium were abundant in the microvillous membrane, the apical tubulo-vacuolar system, and the golgi apparatus, and on the lateral cell and basal membranes, and the luminal surfaces of the capi ... | 1975 | 1234392 |
| dorsal column nuclei projections to the cerebellar cortex in cats as revealed by the use of the retrograde transport of horseradish peroxidase. | the existence of a cerebellar projection from the dorsal column nuclei (gracile and cuneate nuclei, dcn) has been proposed on electrophysiological grounds but questioned when studied with neuroanatomical techniques. the retrograde transport of horseradish peroxidase (hrp) has been used for the present study and provides anatomical evidence of a dcn-cerebellar pathway. in adult cats, 1 to 6 mul of 30% hrp were injected in pars intermedia of the anterior lobe (lobules iv-v), in paramedial lobule a ... | 1975 | 1236873 |
| [exogenous peroxidase in the eggs of rats during cleavage]. | the presence of exogenous peroxidase of the rat's eggs during cleavage at the 1-, 2-, 8-cellular stages and the blastocysts were studied after 30 minute incubation with horseradish peroxidase (molecular weight 40.000, approximate size of the molecule 40 a). the activity of exogenous peroxidase in the area of zona pellucida was established in the perivitelline space, in the cytoplasm - in the pinocytosis vesicles, in certain greater vacuoles and in certain secondary lysosomes. at the stage of bla ... | 1975 | 1241658 |
| in vitro studies on peroxidase movement in the epithelium of prostaglandin-treated rabbit ciliary bodies. | the movement of horseradish peroxidase (hrp) in the epithelium of isolated rabbit iris/ciliary body preparations, has been studied with the electron microscope. hrp was applied at the stromal side of the epithelium, and was left for 60 and 120 min. the distribution pattern of hrp found in the epithelium of the iridial and ciliary processes is consistent with in vivo studies, i.e. the progression of hrp is blocked at the site of the zonula occludens of the superficial epithelium. the hrp distribu ... | 1975 | 1242593 |
| electron microscopic studies on the blood-aqueous barrier of prostaglandin-treated rabbit eyes. i. iridial and ciliary processes. | prostaglandins e1 and e2 were applied topically to rabbit eyes. structures related to the blood-aqueous barrier in the iridial and ciliary processes, as well as the permeability of the ciliary epithelium to the protein tracer horseradish peroxidase, were studied with the electron microscope. marked morphological changes, including dilations of the intercellular spaces and separation of the two epithelial cell layers, were found in the epithelium of the iridial processes. only minor structural ch ... | 1975 | 1242594 |
| electron microscopic studies on the blood-aqueous barrier of prostaglandin-treated rabbit eyes. ii. iris. | the distribution of intravenously injected horseradish peroxidase in the iris of prostaglandin (e1 and e2)-treated rabbit eyes, has been studied with the electron microscope. peroxidase was demonstrated in the stroma of all parts of the iris. the tracer was found throughout interendothelial clefts of iris vessels, indicating that these vessels had become permeable to peroxidase. the distribution of peroxidase in the iris epithelium indicates that the posteroir epithelial cells are girdled by zon ... | 1975 | 1242595 |
| in vitro studies on the selective binding of igg from different species to tissue sections of the bovine mammary gland. | tissue sections of the mammary gland of cattle provide a sensitive and reproducible model for studying the initial events of the selective transport of bovine iggs by the acinar epithelium (ae). mammary tissue was reacted with purified antibody to horseradish peroxidase (hrp) and exposed to hrp. the sites of peroxidase activity were revealed cytochemically. the highly selective binding of iggs by ae of the colostrum-forming gland but never of the lactating gland was most notable, whereas igg1 an ... | 1975 | 11993319 |
| retrograde axonal transport of horseradish peroxidase from spinal cord to brain stem cell groups in the cat. | 1975 | 19604744 | |
| stimulation-induced uptake of horseradish peroxidase by rat cortical synapses. | 1975 | 19604745 | |
| labelling of cells in the medulla oblongata and the spinal cord of the monkey after injections of horseradish peroxidase in the thalamus. | injections of horseradish peroxidase were performed in macaque monkeys in the intralaminar and the ventroposterior thalamic region, respectively. following injection in the intralaminar region labelled cells were present in the marginal zone both in the spinal trigeminal nuclues and in the spinal dorsal horn. the same areas contained labelled cells after injections in the ventroposterior thalamic region, but in such experiments labelled cells were also present in the lamina iv-v region of the sp ... | 1975 | 19604756 |
| the reaction of coumarins with horseradish peroxidase. | the peroxidase catalyzed oxidation of indole-3-acetate is inhibited by naturally occurring coumarins such as scopoletin. this inhibition is due to the preferential reactivity of the coumarins with the peroxidase compounds i, ii, and iii. in view of the possible growth regulatory role of coumarins in plants, the mechanism of oxidation of scopoletin by horse-radish peroxidase has been investigated.peroxidase catalyzed coumarin oxidation requires either an electron donor and molecular oxygen or hyd ... | 1975 | 16659024 |
| the isozymic similarity of indoleacetic acid oxidase to peroxidase in birch and horseradish. | the relationship of indoleacetic acid oxidase activity to peroxidase activity is complicated by numerous multiple forms of this enzyme system. it is not known if all isozymes of this complex system contain both types of activity. isozyme analysis of commercial horseradish peroxidase and leaf extracts of yellow birch (betula alleghaniensis) by isoelectric focusing in polyacrylamide gels was used to examine this problem. horseradish and birch exhibited 20 and 13 peroxidase isozymes, respectively, ... | 1975 | 16659371 |
| nuclear magnetic resonance studies of hemoproteins. binding of aromatic donor molecules to horseradish peroxidase. | 1975 | 429320 | |
| [flavonols and flavones of vegetables. v. flavonols and flavones of root vegetables (author's transl)]. | root vegetables contain flavon(ol) glycosides in tracers up to small amounts, while the level of their leaves are in part considerable (to more than 1 g/kg, calculated as aglycon). radish, rutabagas, scorzoneras, and beets contain less than 1 mg/kg kaempferol and/or quercetin; carrots less than 1 mg/kg apigenin and luteolin; celery roots ca. 75 mg apigenin/kg and 14 mg luteolin/kg; horseradish about 20 mg kaempferol/kg and small radish 1-10 mg kaempferol/kg, whereby all these flavones and flavon ... | 1975 | 1224798 |
| the origin of the annulo-olivary tract with notes on other mesencephalo-olivary pathways. a study by the horseradish peroxidase method. | 1975 | 1182508 | |
| the correlation of the time of origin of neurons with their axonal projection: the combined use of [3h]thymidine autoradiography and horseradish peroxidase histochemistry. | 1975 | 1182549 | |
| anti-horseradish peroxidase associated with tubular structures in the endoplasmic reticulum of plasma cells in stimulated lymph nodes of mice. | tubular structures are described in dilated cisternae of the endoplasmic reticulum in plasma cells of lymph nodes stimulated with horseradish peroxidase (hrp). a positive reaction for anti-hrp antibody suggests that they are formed of immunoglobulins or are at least closely associated with them. | 1975 | 1199967 |
| heme-linked protonation of hcn, co, no and o2 complexes of reduced horseradish peroxidases. | 1975 | 971 | |
| proton magnetic resonance studies of peroxidases from turnip and horseradish. | proton nmr spectra at 270 mhz have been measured for horseradish peroxidase and turnip peroxidase isoenzymes (p1, p2, p3 and p7) in both their high spin ferric native states and as the low spin ferric cyanide complexes. resonances of amino acids near the heme have been identified and used to investigate variations in the structure of the heme crevice amongst the enzymes. ligand proton resonances have been resolved in spectra of the cyanide complexes of the peroxidases and these provide informati ... | 1975 | 172144 |
| the retrograde transport of horseradish peroxidase from the developing limb of the chick embryo. | chick embryos ranging in age from 4.0 to 18 days of incubation and 1-2-day-old hatchlings received injections of hrp solutions directly into the leg musculature. after survival periods of from 5 to 25 h motoneurons and cells in the spinal sensory ganglia were found to be stained with the hrp reaction product. it was found that the first appearance of a positive hrp reaction coincided with the time when nerve processes are first detected in the limb-bud by silver techniques (i.e. at 4.5 days of i ... | 1975 | 1182520 |
| proceedings: spectroscopic and nmr studies on the binding of substrates to horseradish peroxidase. | 1975 | 1205735 | |
| proceedings: modifications of horseradish peroxidase with aromatic compounds. | 1975 | 1205733 | |
| ectopic neurons and aberrant connections during neural development. | during the normal development of the nucleus of origin of centrifugal fibers to the chick retina (the so-called isthmo-optic nucleus) a significant number of neurons are misrouted during their migration from the neural epithelium in which they are generated. like the cells in the isthmo-optic nucleus, these ectopic neurons can be identified by the retrograde transport of horseradish peroxidase (donor: hydrogen peroxide oxidoreductase, ec 1.11.1.7) injected into the eye. between 500 and 600 such ... | 1975 | 1060126 |
| the pinocytic rate of activated macrophages. | peritoneal macrophages from mice injected 4 days previously with brewer's thioglycollate medium have a pinocytic rate, in culture, of 190 ng horseradish peroxidase (hrp)/100 mug cell protein/h, compared to the rate of resident peritoneal cells of 53 ng hrp/100 mug cell protein/h. mice injected with endotoxin or with only certain of the components of the brewer's medium show an intermediate level of stimulation. the rate of unstimulated, endotoxin-stimulated, or thioglycollate-stimulated cells sh ... | 1975 | 53258 |
| the haem-enviroment in horseradish peroxidase as seen by proton magnetic relaxation. | 1975 | 1191283 | |
| circulation and turnover of synaptic vesicle membrane in cultured fetal mammalian spinal cord neurons. | intact neurons in cultures of fetal rodent spinal cord explants show stimulation-dependent uptake of horseradish peroxidase (hrp) into many small vesicles and occasional tubules and multivesicular bodies (mvb) at presynaptic terminals. presynaptic terminals were allowed to take up hrp during 1 h of strychnine-enhanced stimulation of synaptic transmitter release and then "chased" in tracer-free medium either with strychnine or with 10 mm mg++ which depresses transmitter release. tracer-containing ... | 1975 | 1176531 |
| a dual marking technique for microelectrode tracks and localization recording sites. | this paper describes techniques for marking both microelectrode tracks and exact recording loci using a combination of fast green dye and horseradish peroxidase (hrp). the procedure involves coating the exterior of hrp filled microelectrodes with fast green dye in order to identify electrode tracks, and ejecting hrp from the electrode to mark recording loci. rapid, multiple marks can be made with this technique without harming the recording capabilities of the micropipette. | 1975 | 51724 |
| similarities and differences of five peroxidases from turnip and horseradish. peptide mapping studies on glycoproteins. | four isoperoxidases of turnip root and isoperoxidase c of horseradish root were digested with trypsin, and their peptide maps, prepared by high-voltage paper electrophoresis, were compared. all five tryptic digests were completely soluble at ph 8. the maps were developed with a variety of general and specific reagents: ninhydrin, histidine, tyrosine, tryptophan and arginine reagents. cystine peptides and cysteic acid derivatives have also been characterized. all detected half-cystine residues se ... | 1975 | 1175650 |
| an electron microscopic study on the blood-optic nerve and fluid-optic nerve barrier. | utilizing horseradish peroxidase as a tracer, electron microscopic studies were done on the blood-optic nerve and fluid-optic nerve barrier to the peroxidase diffusion. following intravenous injection the peroxidase was observed to fill the lumen of the capillaries of the laminar, prelaminar and orbital portions of the optic nerve but there was no penetratation of the capillary walls. the obstruction of the tracer diffusion out of capillary walls was attributed to the tight junctions between the ... | 1975 | 1082256 |
| oxidase-peroxidase enzymes of datura innoxia. oxidation of formylphenylacetic acid ethyl ester. | an enzyme system from datura innoxia roots oxidizing formylphenylacetic acid ethyl ester was purified 38-fold by conventional methods such as (nh4)2so4 fractionation, negative adsorption on alumina cy gel and chromatography on deae-cellulose. the purified enzyme was shown to catalyse the stoicheiometric oxidation of formylphenylacetic acid ethyl ester to benzoylformic acid ethyl ester and formic acid, utilizing molecular o2. substrate analogues such as phenylacetaldehyde and phenylpyruvate were ... | 1975 | 997 |
| [microenvironment of enzymes as 1 of the factors determining enzyme stability. stabilization of soluble and immobilized horseradish peroxidase]. | 1975 | 223 | |
| the central cervical nucleus - a source of spinocerebellar fibres, demonstrated by retrograde transport of horseradish peroxidase. | injections of horseradish peroxidase in the cerebellum of kittens resulted in labelling of cells in the central cervical nucleus (ccn) in the upper cervical spinal cord. the largest number of labelled ccn neurones was found after injections including the anterior part of the anterior lobe of the cerebellum. in this neuronal system horseradish peroxidase was transported at a maximal velocity of about 150 mm/day. | 1975 | 19604757 |
| preparation and properties of matrix-supported horseradish peroxidase. | a new method of enzyme immobilization has been described using poly(4-methacryloxybenzoic acid) as the carrier. activation of the polymer, prior to enzyme attachment, was achieved with n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. the enzyme coupling step proceeded through nucleophilic attack by the protein on a mixed carbonic anhydride. the degree of polymer activation was determined by analysis for quinoline, a by-product of the reaction. the polymer-enzyme complex was compared to the enzyme ... | 1975 | 241471 |
| ultrastructural localization of in vivo-bound complement in bullous pemphigoid. | antihuman complement component c3 labeled with horseradish peroxidase was used to reveal the ultrastructural localization of complement in two cases of bullous pemphigoid. the complement deposits were shown to be exclusively located in the space between the plasma membrane of the basal cells and the basal lamina. this corresponds exactly to the ultrastructural localization of immunoglobulins in bullous pemphigoid. | 1975 | 50388 |
| an efferent connection of the solitary cells of meynert. a study with horseradish peroxidase in the marmoset callithrix. | 1975 | 809097 | |
| localization of alpha-d-glucosyl and alpha-d-mannosyl groups of mucosubstances with concanavalin a and horseradish peroxidase. | concanavalin a is a lectin which is known to bind specifically to alpha-d-glucosyl and alpha-d-mannosyl groups in the mucosubstances of mammalian tissues. the lectin molecule is bivalent; after its attachment to mucosubstances present in a histological specimen it can also bind horseradish peroxidase, a mannose-containing glycoprotein. the attached peroxidase may then be visualized by virtue of its histochemically demonstrable enzymatic activity. other investigators have utilized this principle ... | 1975 | 52638 |